CG7300 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG7300
Entrez Gene ID	34472
Full Name	CG7300 gene product from transcript CG7300-RD
Synonyms	Dmel\CG7300
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 32A4-32A4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001201860.2	NP_001188789.2	uncharacterized protein, isoform D
NM_135596.4	NP_609440.2	uncharacterized protein, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG7300 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG7300 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14394	NM_001201860.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant D (CG7300), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OFa14394	NM_135596.4 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG7300), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG7300 ( NM_001201860.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001188789.2 Drosophila melanogaster uncharacterized protein, transcript variant D (CG7300), mRNA.

Price & Availability↑

CloneID	OFa14394
Clone ID Related Accession (Same CDS sequence)	NM_001201860.2 , NM_135596.4
Accession Version	NM_001201860.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2661bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001201860.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTCCCA CGGGATTGTA TTTACTTCTA ACTGCCTGCT GCGTATCCTT GGCATCCGCC 
GGATTCGGAC TGGGCTTCGG TGGTGCTAGC CTTGGATTTG GAGGCGGAAA CGGTGGTGGC 
TCGGCTCAAG CAAGTCTGCC TGGATTGAGT CTGGGTGGGG AATACAACAG AGGATCTTCT 
GGATCGGTTC TGAATCAGGC TGCCAATGGC ATTCTGGGCG CTGCTTCGAC TGGAGCCAAT 
GTCGTCCACT CTGCCGGATC CGCAGCTGCT AGTCACGGAG CTCAGGCTGC CAATGCAGTG 
CTAGGAGCAG CTGGATCTGG AGTAGATGCC GTCCATTCTG CGGGATCAAC AGCTGCTGCT 
CACGGAGCTC AGGCAGTGAA CGGGATATTG GGCGCTGCTT CGACTGGAGC CAATGTCGTT 
CATTCCGCCG GATCCGCAGC TGCCAATCAC GGAGCTCAGG CTGCCAATGC AGTGCTAGGA 
GCAGCTGGAT CTGGAGTAGA TGCCGTCCAC TCTGCGGGAT CAACAGCTGC TGCTCACGGA 
GCTCAGGCAG TGAACGGGAT ATTGGGCGCT GCTTCGACTG GAGCCAATGT CGTTCATTCC 
GCCGGATCCG CAGCTGCCAA TCACGGAGCT CAGGCTGCCC ATGGAGTGCT AGGAGCAACT 
GGATCTGGAG TAGATGCAAT CCATTCAGCG GGATCAACAG CTGCTGCTCA CGGAGCTCAG 
GCAGTGAACG GAATTTTGGG AGTGGCCGGA ACTGGAGCTA ATGTTGTGCA CTCGGCAGGA 
TCGACGGCCG CATCTTACGG AGCACATGCT GCTCACGGAG TTTTGGGAGC TGCTGCGACT 
GGAGTCAATG CTGTGCATTC TGCAGGTGTA ACGGCGGCGG ATGAGGTACA CAAAGCGTCT 
TCTCAGCACA GTGGGTCAGG ATCTGCTTCC CTAGGAGTTG GACATTTGAA TATTCAAACC 
TCTGGCAACA GTGGTAGCAA GACGAATACT GCACACTTGA CCATCGGCAC TTCCGGCGTC 
TCGGGTTCCA GTGACCTTCA TCATAGCGCC GGATCAGCAA CTGTTAATGC AGCCAATGCA 
GTGCTGAATG CAGCGGAAGC TGGAGCAGCA GGATCGACAG GTGCCGCTCA TGGAGCACAG 
GTTGTCAACG GAGTTTTGGG AGTTGCAGGA ACCGGAGCCA ATGTTATCCA TTCCGCAGGA 
ACAATTACTG CCGCTCATGG AGCTCAGGCA GCAGACACAG TTGCCAGTGG CGTTGTCAAC 
GGTGCAACCT CTGGTTCCGG AGCCAATTCT GGTCACTTGA GCATCGGATC ATCTGGAATT 
TCCGGATCCA GCTCAGGCTC CCTTGGCAAT GCCAAAATCA CTGGATCTTC GTCAACTGGT 
GCACACCATA CTGGATCTGT TCAGGCTGGT TCTGCTAAGA TCACAGCTGG ACATGGGAAA 
ATCGAAACCT CTGGCTCGAA TTCCGGCACT GCTGGCCACA TTAATGTAGA CGCCACTGGA 
GCCAGTGAAA AGAAAACTTC TTCTTCATAT CATAAATCTG AGTCCACGAT GGTCTCTTCT 
GGGCATTCAT CCACTTATTC CTCAAAAACC GAATCATCCA AATTCAGTGC TGGTCAATCC 
CACATTAGTT CTGGATCCGC AACCATTGGA GCCTCTGGAA CGCACGCTGC ATCTGCCCAC 
ATCAAGACAG AAAACAAGAA AGTTGAGTCA TCGGGATCAA GCTCTATGCA CGCCGGATCA 
GGTTCTGCTT CCATCGGTGC TGGTCATCTG AATGTCCAAT CTGCCGGAAC TACCGGATCC 
TCCGAAGCTG CTTCAAACCA CGCATCTATT GGTGTAGGAC ACCTGAACAT TGGAGCAACT 
GATTCCGCCT CTAAGGGATC CTCTGCCCTC GGTTCATCTG GCCACTTGAG CATCGGATCA 
GCTGGAATCT CAGGATCTGC TGGTTCTGGA GCCTCTGGAT CACACACTGG ATCTGCCCAC 
ATCAAGACAG AGCACAAGAA AGTTGAGTCA TCGGGATCTA GTTCCCTGCA CACCGGATCA 
GGTTCTGCCT CCATCGAAGC TGGTCATCTA AATATCCAAT CTGCTGGAAG TACCGGTTCC 
TCTCAAGCTG CATCTATTGG TGTCGGACAT CTGAGCATTG GATCAACTGA TTCCGCTTCT 
AAGGGATCCT CTGGCACTGG ATCATCTGGT CACTTGAGCA TCGGATCAGT TGGAATCTCT 
GGATCTACTG GCTCTGGCGC CGGATCATCC AAGATCAACG CCGGACATTT GAGTATCGGA 
ACCTCTGGAA ATTCAGGATC TGCTTCACTT GGAACAAATG GCCTGTCAAT AGGAGCAAAC 
GGACACAACT CTAACACAGC TGGATCGATT GGACTAGCCG GTGCCCACGA GGCTGCGTCT 
CTGGCCCATG GAGTCGCCAG TGGTATACTG ACCCCTGCTG TGGGTTCGGG AAGTCTCTCT 
CTCGGTGGTA GCACTGCCTC CTTGGGATCG AGCGGATCTG CCAGCCAAGG AGGATATCGC 
CAGGGAAGTA TCAGTCTGGG AGGAAATGGT CTAAGCCTTG GTGGCAATCT CGGCGGGAAC 
TCTGGAAGTG CCGGAATTGG ATTGGGTGGC GCTCACCTTG GAGGATCCAT TAACCGTGGC 
GGCGGCCTTT TCCTTGGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188789.2
CDS	20..2680
Translation	MAPTGLYLLLTACCVSLASAGFGLGFGGASLGFGGGNGGGSAQASLPGLSLGGEYNRGSSGSVLNQAANGILGAASTGANVVHSAGSAAASHGAQAANAVLGAAGSGVDAVHSAGSTAAAHGAQAVNGILGAASTGANVVHSAGSAAANHGAQAANAVLGAAGSGVDAVHSAGSTAAAHGAQAVNGILGAASTGANVVHSAGSAAANHGAQAAHGVLGATGSGVDAIHSAGSTAAAHGAQAVNGILGVAGTGANVVHSAGSTAASYGAHAAHGVLGAAATGVNAVHSAGVTAADEVHKASSQHSGSGSASLGVGHLNIQTSGNSGSKTNTAHLTIGTSGVSGSSDLHHSAGSATVNAANAVLNAAEAGAAGSTGAAHGAQVVNGVLGVAGTGANVIHSAGTITAAHGAQAADTVASGVVNGATSGSGANSGHLSIGSSGISGSSSGSLGNAKITGSSSTGAHHTGSVQAGSAKITAGHGKIETSGSNSGTAGHINVDATGASEKKTSSSYHKSESTMVSSGHSSTYSSKTESSKFSAGQSHISSGSATIGASGTHAASAHIKTENKKVESSGSSSMHAGSGSASIGAGHLNVQSAGTTGSSEAASNHASIGVGHLNIGATDSASKGSSALGSSGHLSIGSAGISGSAGSGASGSHTGSAHIKTEHKKVESSGSSSLHTGSGSASIEAGHLNIQSAGSTGSSQAASIGVGHLSIGSTDSASKGSSGTGSSGHLSIGSVGISGSTGSGAGSSKINAGHLSIGTSGNSGSASLGTNGLSIGANGHNSNTAGSIGLAGAHEAASLAHGVASGILTPAVGSGSLSLGGSTASLGSSGSASQGGYRQGSISLGGNGLSLGGNLGGNSGSAGIGLGGAHLGGSINRGGGLFLG

Target ORF information:

RefSeq Version	NM_001201860.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant D (CG7300), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201860.2

ORF Insert Sequence:

ATGGCTCCCA CGGGATTGTA TTTACTTCTA ACTGCCTGCT GCGTATCCTT GGCATCCGCC 
GGATTCGGAC TGGGCTTCGG TGGTGCTAGC CTTGGATTTG GAGGCGGAAA CGGTGGTGGC 
TCGGCTCAAG CAAGTCTGCC TGGATTGAGT CTGGGTGGGG AATACAACAG AGGATCTTCT 
GGATCGGTTC TGAATCAGGC TGCCAATGGC ATTCTGGGCG CTGCTTCGAC TGGAGCCAAT 
GTCGTCCACT CTGCCGGATC CGCAGCTGCT AGTCACGGAG CTCAGGCTGC CAATGCAGTG 
CTAGGAGCAG CTGGATCTGG AGTAGATGCC GTCCATTCTG CGGGATCAAC AGCTGCTGCT 
CACGGAGCTC AGGCAGTGAA CGGGATATTG GGCGCTGCTT CGACTGGAGC CAATGTCGTT 
CATTCCGCCG GATCCGCAGC TGCCAATCAC GGAGCTCAGG CTGCCAATGC AGTGCTAGGA 
GCAGCTGGAT CTGGAGTAGA TGCCGTCCAC TCTGCGGGAT CAACAGCTGC TGCTCACGGA 
GCTCAGGCAG TGAACGGGAT ATTGGGCGCT GCTTCGACTG GAGCCAATGT CGTTCATTCC 
GCCGGATCCG CAGCTGCCAA TCACGGAGCT CAGGCTGCCC ATGGAGTGCT AGGAGCAACT 
GGATCTGGAG TAGATGCAAT CCATTCAGCG GGATCAACAG CTGCTGCTCA CGGAGCTCAG 
GCAGTGAACG GAATTTTGGG AGTGGCCGGA ACTGGAGCTA ATGTTGTGCA CTCGGCAGGA 
TCGACGGCCG CATCTTACGG AGCACATGCT GCTCACGGAG TTTTGGGAGC TGCTGCGACT 
GGAGTCAATG CTGTGCATTC TGCAGGTGTA ACGGCGGCGG ATGAGGTACA CAAAGCGTCT 
TCTCAGCACA GTGGGTCAGG ATCTGCTTCC CTAGGAGTTG GACATTTGAA TATTCAAACC 
TCTGGCAACA GTGGTAGCAA GACGAATACT GCACACTTGA CCATCGGCAC TTCCGGCGTC 
TCGGGTTCCA GTGACCTTCA TCATAGCGCC GGATCAGCAA CTGTTAATGC AGCCAATGCA 
GTGCTGAATG CAGCGGAAGC TGGAGCAGCA GGATCGACAG GTGCCGCTCA TGGAGCACAG 
GTTGTCAACG GAGTTTTGGG AGTTGCAGGA ACCGGAGCCA ATGTTATCCA TTCCGCAGGA 
ACAATTACTG CCGCTCATGG AGCTCAGGCA GCAGACACAG TTGCCAGTGG CGTTGTCAAC 
GGTGCAACCT CTGGTTCCGG AGCCAATTCT GGTCACTTGA GCATCGGATC ATCTGGAATT 
TCCGGATCCA GCTCAGGCTC CCTTGGCAAT GCCAAAATCA CTGGATCTTC GTCAACTGGT 
GCACACCATA CTGGATCTGT TCAGGCTGGT TCTGCTAAGA TCACAGCTGG ACATGGGAAA 
ATCGAAACCT CTGGCTCGAA TTCCGGCACT GCTGGCCACA TTAATGTAGA CGCCACTGGA 
GCCAGTGAAA AGAAAACTTC TTCTTCATAT CATAAATCTG AGTCCACGAT GGTCTCTTCT 
GGGCATTCAT CCACTTATTC CTCAAAAACC GAATCATCCA AATTCAGTGC TGGTCAATCC 
CACATTAGTT CTGGATCCGC AACCATTGGA GCCTCTGGAA CGCACGCTGC ATCTGCCCAC 
ATCAAGACAG AAAACAAGAA AGTTGAGTCA TCGGGATCAA GCTCTATGCA CGCCGGATCA 
GGTTCTGCTT CCATCGGTGC TGGTCATCTG AATGTCCAAT CTGCCGGAAC TACCGGATCC 
TCCGAAGCTG CTTCAAACCA CGCATCTATT GGTGTAGGAC ACCTGAACAT TGGAGCAACT 
GATTCCGCCT CTAAGGGATC CTCTGCCCTC GGTTCATCTG GCCACTTGAG CATCGGATCA 
GCTGGAATCT CAGGATCTGC TGGTTCTGGA GCCTCTGGAT CACACACTGG ATCTGCCCAC 
ATCAAGACAG AGCACAAGAA AGTTGAGTCA TCGGGATCTA GTTCCCTGCA CACCGGATCA 
GGTTCTGCCT CCATCGAAGC TGGTCATCTA AATATCCAAT CTGCTGGAAG TACCGGTTCC 
TCTCAAGCTG CATCTATTGG TGTCGGACAT CTGAGCATTG GATCAACTGA TTCCGCTTCT 
AAGGGATCCT CTGGCACTGG ATCATCTGGT CACTTGAGCA TCGGATCAGT TGGAATCTCT 
GGATCTACTG GCTCTGGCGC CGGATCATCC AAGATCAACG CCGGACATTT GAGTATCGGA 
ACCTCTGGAA ATTCAGGATC TGCTTCACTT GGAACAAATG GCCTGTCAAT AGGAGCAAAC 
GGACACAACT CTAACACAGC TGGATCGATT GGACTAGCCG GTGCCCACGA GGCTGCGTCT 
CTGGCCCATG GAGTCGCCAG TGGTATACTG ACCCCTGCTG TGGGTTCGGG AAGTCTCTCT 
CTCGGTGGTA GCACTGCCTC CTTGGGATCG AGCGGATCTG CCAGCCAAGG AGGATATCGC 
CAGGGAAGTA TCAGTCTGGG AGGAAATGGT CTAAGCCTTG GTGGCAATCT CGGCGGGAAC 
TCTGGAAGTG CCGGAATTGG ATTGGGTGGC GCTCACCTTG GAGGATCCAT TAACCGTGGC 
GGCGGCCTTT TCCTTGGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG7300 ( NM_135596.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609440.2 Drosophila melanogaster uncharacterized protein, transcript variant B (CG7300), mRNA.

Price & Availability↑

CloneID	OFa14394
Clone ID Related Accession (Same CDS sequence)	NM_001201860.2 , NM_135596.4
Accession Version	NM_135596.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2661bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_135596.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTCCCA CGGGATTGTA TTTACTTCTA ACTGCCTGCT GCGTATCCTT GGCATCCGCC 
GGATTCGGAC TGGGCTTCGG TGGTGCTAGC CTTGGATTTG GAGGCGGAAA CGGTGGTGGC 
TCGGCTCAAG CAAGTCTGCC TGGATTGAGT CTGGGTGGGG AATACAACAG AGGATCTTCT 
GGATCGGTTC TGAATCAGGC TGCCAATGGC ATTCTGGGCG CTGCTTCGAC TGGAGCCAAT 
GTCGTCCACT CTGCCGGATC CGCAGCTGCT AGTCACGGAG CTCAGGCTGC CAATGCAGTG 
CTAGGAGCAG CTGGATCTGG AGTAGATGCC GTCCATTCTG CGGGATCAAC AGCTGCTGCT 
CACGGAGCTC AGGCAGTGAA CGGGATATTG GGCGCTGCTT CGACTGGAGC CAATGTCGTT 
CATTCCGCCG GATCCGCAGC TGCCAATCAC GGAGCTCAGG CTGCCAATGC AGTGCTAGGA 
GCAGCTGGAT CTGGAGTAGA TGCCGTCCAC TCTGCGGGAT CAACAGCTGC TGCTCACGGA 
GCTCAGGCAG TGAACGGGAT ATTGGGCGCT GCTTCGACTG GAGCCAATGT CGTTCATTCC 
GCCGGATCCG CAGCTGCCAA TCACGGAGCT CAGGCTGCCC ATGGAGTGCT AGGAGCAACT 
GGATCTGGAG TAGATGCAAT CCATTCAGCG GGATCAACAG CTGCTGCTCA CGGAGCTCAG 
GCAGTGAACG GAATTTTGGG AGTGGCCGGA ACTGGAGCTA ATGTTGTGCA CTCGGCAGGA 
TCGACGGCCG CATCTTACGG AGCACATGCT GCTCACGGAG TTTTGGGAGC TGCTGCGACT 
GGAGTCAATG CTGTGCATTC TGCAGGTGTA ACGGCGGCGG ATGAGGTACA CAAAGCGTCT 
TCTCAGCACA GTGGGTCAGG ATCTGCTTCC CTAGGAGTTG GACATTTGAA TATTCAAACC 
TCTGGCAACA GTGGTAGCAA GACGAATACT GCACACTTGA CCATCGGCAC TTCCGGCGTC 
TCGGGTTCCA GTGACCTTCA TCATAGCGCC GGATCAGCAA CTGTTAATGC AGCCAATGCA 
GTGCTGAATG CAGCGGAAGC TGGAGCAGCA GGATCGACAG GTGCCGCTCA TGGAGCACAG 
GTTGTCAACG GAGTTTTGGG AGTTGCAGGA ACCGGAGCCA ATGTTATCCA TTCCGCAGGA 
ACAATTACTG CCGCTCATGG AGCTCAGGCA GCAGACACAG TTGCCAGTGG CGTTGTCAAC 
GGTGCAACCT CTGGTTCCGG AGCCAATTCT GGTCACTTGA GCATCGGATC ATCTGGAATT 
TCCGGATCCA GCTCAGGCTC CCTTGGCAAT GCCAAAATCA CTGGATCTTC GTCAACTGGT 
GCACACCATA CTGGATCTGT TCAGGCTGGT TCTGCTAAGA TCACAGCTGG ACATGGGAAA 
ATCGAAACCT CTGGCTCGAA TTCCGGCACT GCTGGCCACA TTAATGTAGA CGCCACTGGA 
GCCAGTGAAA AGAAAACTTC TTCTTCATAT CATAAATCTG AGTCCACGAT GGTCTCTTCT 
GGGCATTCAT CCACTTATTC CTCAAAAACC GAATCATCCA AATTCAGTGC TGGTCAATCC 
CACATTAGTT CTGGATCCGC AACCATTGGA GCCTCTGGAA CGCACGCTGC ATCTGCCCAC 
ATCAAGACAG AAAACAAGAA AGTTGAGTCA TCGGGATCAA GCTCTATGCA CGCCGGATCA 
GGTTCTGCTT CCATCGGTGC TGGTCATCTG AATGTCCAAT CTGCCGGAAC TACCGGATCC 
TCCGAAGCTG CTTCAAACCA CGCATCTATT GGTGTAGGAC ACCTGAACAT TGGAGCAACT 
GATTCCGCCT CTAAGGGATC CTCTGCCCTC GGTTCATCTG GCCACTTGAG CATCGGATCA 
GCTGGAATCT CAGGATCTGC TGGTTCTGGA GCCTCTGGAT CACACACTGG ATCTGCCCAC 
ATCAAGACAG AGCACAAGAA AGTTGAGTCA TCGGGATCTA GTTCCCTGCA CACCGGATCA 
GGTTCTGCCT CCATCGAAGC TGGTCATCTA AATATCCAAT CTGCTGGAAG TACCGGTTCC 
TCTCAAGCTG CATCTATTGG TGTCGGACAT CTGAGCATTG GATCAACTGA TTCCGCTTCT 
AAGGGATCCT CTGGCACTGG ATCATCTGGT CACTTGAGCA TCGGATCAGT TGGAATCTCT 
GGATCTACTG GCTCTGGCGC CGGATCATCC AAGATCAACG CCGGACATTT GAGTATCGGA 
ACCTCTGGAA ATTCAGGATC TGCTTCACTT GGAACAAATG GCCTGTCAAT AGGAGCAAAC 
GGACACAACT CTAACACAGC TGGATCGATT GGACTAGCCG GTGCCCACGA GGCTGCGTCT 
CTGGCCCATG GAGTCGCCAG TGGTATACTG ACCCCTGCTG TGGGTTCGGG AAGTCTCTCT 
CTCGGTGGTA GCACTGCCTC CTTGGGATCG AGCGGATCTG CCAGCCAAGG AGGATATCGC 
CAGGGAAGTA TCAGTCTGGG AGGAAATGGT CTAAGCCTTG GTGGCAATCT CGGCGGGAAC 
TCTGGAAGTG CCGGAATTGG ATTGGGTGGC GCTCACCTTG GAGGATCCAT TAACCGTGGC 
GGCGGCCTTT TCCTTGGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609440.2
CDS	20..2680
Translation	MAPTGLYLLLTACCVSLASAGFGLGFGGASLGFGGGNGGGSAQASLPGLSLGGEYNRGSSGSVLNQAANGILGAASTGANVVHSAGSAAASHGAQAANAVLGAAGSGVDAVHSAGSTAAAHGAQAVNGILGAASTGANVVHSAGSAAANHGAQAANAVLGAAGSGVDAVHSAGSTAAAHGAQAVNGILGAASTGANVVHSAGSAAANHGAQAAHGVLGATGSGVDAIHSAGSTAAAHGAQAVNGILGVAGTGANVVHSAGSTAASYGAHAAHGVLGAAATGVNAVHSAGVTAADEVHKASSQHSGSGSASLGVGHLNIQTSGNSGSKTNTAHLTIGTSGVSGSSDLHHSAGSATVNAANAVLNAAEAGAAGSTGAAHGAQVVNGVLGVAGTGANVIHSAGTITAAHGAQAADTVASGVVNGATSGSGANSGHLSIGSSGISGSSSGSLGNAKITGSSSTGAHHTGSVQAGSAKITAGHGKIETSGSNSGTAGHINVDATGASEKKTSSSYHKSESTMVSSGHSSTYSSKTESSKFSAGQSHISSGSATIGASGTHAASAHIKTENKKVESSGSSSMHAGSGSASIGAGHLNVQSAGTTGSSEAASNHASIGVGHLNIGATDSASKGSSALGSSGHLSIGSAGISGSAGSGASGSHTGSAHIKTEHKKVESSGSSSLHTGSGSASIEAGHLNIQSAGSTGSSQAASIGVGHLSIGSTDSASKGSSGTGSSGHLSIGSVGISGSTGSGAGSSKINAGHLSIGTSGNSGSASLGTNGLSIGANGHNSNTAGSIGLAGAHEAASLAHGVASGILTPAVGSGSLSLGGSTASLGSSGSASQGGYRQGSISLGGNGLSLGGNLGGNSGSAGIGLGGAHLGGSINRGGGLFLG

Target ORF information:

RefSeq Version	NM_135596.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG7300), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135596.4

ORF Insert Sequence:

ATGGCTCCCA CGGGATTGTA TTTACTTCTA ACTGCCTGCT GCGTATCCTT GGCATCCGCC 
GGATTCGGAC TGGGCTTCGG TGGTGCTAGC CTTGGATTTG GAGGCGGAAA CGGTGGTGGC 
TCGGCTCAAG CAAGTCTGCC TGGATTGAGT CTGGGTGGGG AATACAACAG AGGATCTTCT 
GGATCGGTTC TGAATCAGGC TGCCAATGGC ATTCTGGGCG CTGCTTCGAC TGGAGCCAAT 
GTCGTCCACT CTGCCGGATC CGCAGCTGCT AGTCACGGAG CTCAGGCTGC CAATGCAGTG 
CTAGGAGCAG CTGGATCTGG AGTAGATGCC GTCCATTCTG CGGGATCAAC AGCTGCTGCT 
CACGGAGCTC AGGCAGTGAA CGGGATATTG GGCGCTGCTT CGACTGGAGC CAATGTCGTT 
CATTCCGCCG GATCCGCAGC TGCCAATCAC GGAGCTCAGG CTGCCAATGC AGTGCTAGGA 
GCAGCTGGAT CTGGAGTAGA TGCCGTCCAC TCTGCGGGAT CAACAGCTGC TGCTCACGGA 
GCTCAGGCAG TGAACGGGAT ATTGGGCGCT GCTTCGACTG GAGCCAATGT CGTTCATTCC 
GCCGGATCCG CAGCTGCCAA TCACGGAGCT CAGGCTGCCC ATGGAGTGCT AGGAGCAACT 
GGATCTGGAG TAGATGCAAT CCATTCAGCG GGATCAACAG CTGCTGCTCA CGGAGCTCAG 
GCAGTGAACG GAATTTTGGG AGTGGCCGGA ACTGGAGCTA ATGTTGTGCA CTCGGCAGGA 
TCGACGGCCG CATCTTACGG AGCACATGCT GCTCACGGAG TTTTGGGAGC TGCTGCGACT 
GGAGTCAATG CTGTGCATTC TGCAGGTGTA ACGGCGGCGG ATGAGGTACA CAAAGCGTCT 
TCTCAGCACA GTGGGTCAGG ATCTGCTTCC CTAGGAGTTG GACATTTGAA TATTCAAACC 
TCTGGCAACA GTGGTAGCAA GACGAATACT GCACACTTGA CCATCGGCAC TTCCGGCGTC 
TCGGGTTCCA GTGACCTTCA TCATAGCGCC GGATCAGCAA CTGTTAATGC AGCCAATGCA 
GTGCTGAATG CAGCGGAAGC TGGAGCAGCA GGATCGACAG GTGCCGCTCA TGGAGCACAG 
GTTGTCAACG GAGTTTTGGG AGTTGCAGGA ACCGGAGCCA ATGTTATCCA TTCCGCAGGA 
ACAATTACTG CCGCTCATGG AGCTCAGGCA GCAGACACAG TTGCCAGTGG CGTTGTCAAC 
GGTGCAACCT CTGGTTCCGG AGCCAATTCT GGTCACTTGA GCATCGGATC ATCTGGAATT 
TCCGGATCCA GCTCAGGCTC CCTTGGCAAT GCCAAAATCA CTGGATCTTC GTCAACTGGT 
GCACACCATA CTGGATCTGT TCAGGCTGGT TCTGCTAAGA TCACAGCTGG ACATGGGAAA 
ATCGAAACCT CTGGCTCGAA TTCCGGCACT GCTGGCCACA TTAATGTAGA CGCCACTGGA 
GCCAGTGAAA AGAAAACTTC TTCTTCATAT CATAAATCTG AGTCCACGAT GGTCTCTTCT 
GGGCATTCAT CCACTTATTC CTCAAAAACC GAATCATCCA AATTCAGTGC TGGTCAATCC 
CACATTAGTT CTGGATCCGC AACCATTGGA GCCTCTGGAA CGCACGCTGC ATCTGCCCAC 
ATCAAGACAG AAAACAAGAA AGTTGAGTCA TCGGGATCAA GCTCTATGCA CGCCGGATCA 
GGTTCTGCTT CCATCGGTGC TGGTCATCTG AATGTCCAAT CTGCCGGAAC TACCGGATCC 
TCCGAAGCTG CTTCAAACCA CGCATCTATT GGTGTAGGAC ACCTGAACAT TGGAGCAACT 
GATTCCGCCT CTAAGGGATC CTCTGCCCTC GGTTCATCTG GCCACTTGAG CATCGGATCA 
GCTGGAATCT CAGGATCTGC TGGTTCTGGA GCCTCTGGAT CACACACTGG ATCTGCCCAC 
ATCAAGACAG AGCACAAGAA AGTTGAGTCA TCGGGATCTA GTTCCCTGCA CACCGGATCA 
GGTTCTGCCT CCATCGAAGC TGGTCATCTA AATATCCAAT CTGCTGGAAG TACCGGTTCC 
TCTCAAGCTG CATCTATTGG TGTCGGACAT CTGAGCATTG GATCAACTGA TTCCGCTTCT 
AAGGGATCCT CTGGCACTGG ATCATCTGGT CACTTGAGCA TCGGATCAGT TGGAATCTCT 
GGATCTACTG GCTCTGGCGC CGGATCATCC AAGATCAACG CCGGACATTT GAGTATCGGA 
ACCTCTGGAA ATTCAGGATC TGCTTCACTT GGAACAAATG GCCTGTCAAT AGGAGCAAAC 
GGACACAACT CTAACACAGC TGGATCGATT GGACTAGCCG GTGCCCACGA GGCTGCGTCT 
CTGGCCCATG GAGTCGCCAG TGGTATACTG ACCCCTGCTG TGGGTTCGGG AAGTCTCTCT 
CTCGGTGGTA GCACTGCCTC CTTGGGATCG AGCGGATCTG CCAGCCAAGG AGGATATCGC 
CAGGGAAGTA TCAGTCTGGG AGGAAATGGT CTAAGCCTTG GTGGCAATCT CGGCGGGAAC 
TCTGGAAGTG CCGGAATTGG ATTGGGTGGC GCTCACCTTG GAGGATCCAT TAACCGTGGC 
GGCGGCCTTT TCCTTGGCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG7300 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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