LManI cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LManI
Entrez Gene ID	34436
Full Name	Lysosomal alpha-mannosidase I
Synonyms	CG5322,Dmel\CG5322
General protein information	Preferred Names Lysosomal alpha-mannosidase I Names CG5322 gene product from transcript CG5322-RB CG5322-PA CG5322-PB LManI-PA LManI-PB lysosomal alpha-mannosidase I
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 31E4-31E5

mRNA and Protein(s)

mRNA	Protein	Name
NM_135563.3	NP_609407.1	lysosomal alpha-mannosidase I, isoform A
NM_001273421.1	NP_001260350.1	lysosomal alpha-mannosidase I, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG9468	NP_609253.1
Drosophila melanogaster (fruit fly)	CG9466	NP_609252.1
Drosophila melanogaster (fruit fly)	CG5322	NP_609407.1
Drosophila melanogaster (fruit fly)	CG9463	NP_609250.2
Drosophila melanogaster (fruit fly)	CG9465	NP_609251.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LManI gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LManI cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14361	NM_001273421.1 Latest version!	Drosophila melanogaster lysosomal alpha-mannosidase I, transcript variant B (LManI), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa14361	NM_135563.3 Latest version!	Drosophila melanogaster lysosomal alpha-mannosidase I, transcript variant A (LManI), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LManI ( NM_135563.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609407.1 Drosophila melanogaster lysosomal alpha-mannosidase I, transcript variant A (LManI), mRNA.

Price & Availability↑

CloneID	OFa14361
Clone ID Related Accession (Same CDS sequence)	NM_135563.3 , NM_001273421.1
Accession Version	NM_135563.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lysosomal alpha-mannosidase I, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_135563.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATCAATG TGCACTTGGT GCCGCATTCG CACGACGACG TTGGATGGCT AAAGACAGTG 
GATCAGTATT ATTATGGTAG TCAGAACAAG ATACAACACG CTGGAGTCCA ATACATTTTG 
GACACCGTGG TGGAGGAGCT CCTCAAGGAT TCGTCGAGGC GCTTCATCCA GGTGGAAACG 
TTTTTCTTTG CAAAATGGTA TTCGGAGCAA ACTGAAACGG TCCAAAGGGC GGTGAAAAAG 
TTGGTGGCCC AAGGACGTTT GGAATTCGCT GGTGGAGCTT GGAGCATGAA CGATGAGGCC 
ACAGTTCACT ATCAAAGTGT TGTAGATCAG TTTAACCTGG GCTTAAGATA TCTTAAAGAT 
ACTTTTGGTG ATTGTGGACG CCCCACGGTT GGTTGGCAGA TCGATCCATT TGGTCATTCC 
CGAGAAATGG CCTCCATATT CGCACAAATG GCATTTAATG GTGAATTTTT CGCACGCATG 
GACTACGTTG ATAAGAAGCA ACGTATGCTG GATCTGGAAA TGGAGATGAT TTGGCAGTCC 
AGCGAGTCCC TAAAGAATTC GAATATATTC ACTGGCATGC TCTACAATCA CTATTCAGCT 
CCTCCGGGAT TCTGTTTCGA TATCAATTGC GAGGATGCAC CAATTATCGA TGGCGAGAGC 
TATGATAATA ACGTGGATGC GAGAGTCAGT GAGTTTATAG ACTATGTTAA GAACATGTCT 
AAGTCGTATC GATCCACTCA CATCATGGTG CCCATGGGAG ATGATTTTCA GTACGAAGAT 
GCAGCAGTTA ACTTCAAGAA TATGGACAAA CTTATCAAGT ACGTCAACGA TCGCCAATCG 
ACGGGATCCC AAGTCAACGT ATTCTACTCC ACACCCTCGT GTTATCTTTA TGAATTGCAC 
CAATTGAAGC AAACTTGGCC AAACAAAACG GAAGACTTCT TTCCATATTC CAGTGATTCG 
CACTCCTACT GGACCGGATA CTTCACCTCA CGACCCACCC AAAAACGTTT CCACAGGGAT 
GGCAATCACT TCTTCCAAAC TGTTAAGCAA TTGAGTGTGT TGGCCAACTT GAGTGGCACC 
CAGTACTCCG AAGATCTGGA TAATCTGAGT CAGGCAATGG GCATAATGCA GCATCATGAT 
GCTGTTACGG GTACCGAAAA ACAGGCGGTG GCCAGCGACT ATGATCGCTT ATTGTTCAAG 
GCCATCGTTG GAGCTGAAAA CAGTGCCCGA GATGCCCTAC GATCTCTGAC CAATCTGACC 
TCAGGGGAGT TCGAGAGTTG CCTGGAACTC AACATAAGTG TTTGTGCCTT CACCCAAGAT 
ACCGCCAACA ATGTGATTGT GACCCTGGTC AATCCATTGG CTCATTCTTC AACACAATAT 
GTGCGTGTTC CGGCGAAGAA CGAGAACTAC ATAGTCACCG ATGAGAAAGG TCGTGAAGTA 
TTTTCCGAAG TGGTTCCCGT TCCCTGGCAA GTTTTGGCGT TGGAACATCG ACCTAATGAT 
ACCCAGCACG AGTTGGTTTT TGAAGCCAGT GTTGATAAAA TAGCCAACTT TTTGATTCGT 
GTGCTACCAT CACCGAAAAA TATTGCAGAA GATCAAGTCC AATTCCCGGT AGAGAGGAGC 
CAGGATGAGT TAACAGTGGA GACTTCGCTG GTAAAACTGA CTTTTGATAC CACAACGGGT 
GGCTTGAAAA CTGTGAAAAT GAACGGATTT ACGGAAAATA TTCAACAGAC CTTTGGTATA 
TACAAGGGTT ATCGCGGCAA TAATGGTGAA TCGAAAAACC GATCATCCGG TGCCTATGTT 
TTCCGTCCAT ATGGCGATAT TGAAATTGTG AACAACAAGG TGGAGCTATC ATTCTATAAT 
GGCACCAAGG TTAAGGAAGT CCATCAGCAT GTCAACGAAT GGATCTCCCA GGTAATCCGA 
ATCTATGAGG ACGTCAATCG TGTTGAATTT GAATGGCTAG TCGGACCGAT TCCAACGGAT 
GATGATGTTG GCAAGGAGAT AATCACCCGG TTTTCCAGCA ACATATCCTC GAAGGGCAAG 
TTCTACACGG ACTCAAATGG TCGTGAAATC CTGGAACGTG AGAGAAATCA GCGGGAGCAC 
TTCACTCCGG ATATGTCGGA GGCTATAAGT GGAAACTATT ACCCGGTGAC TGGTCAAATT 
TCGTTACAAG ATGACGAAAA GCGAATCACC CTGCTGAATG ATAGAGCCCA GGGTGGAACC 
AGCCTAAAGG ATGGCGAACT GGAGCTGATG CTCCATCGTC GCCTTCTGAA CGATGATGCG 
TTTGGAGTGG GCGAAGCCCT AAATGAGACG CAATATGGAA CTGGTTTGAT TGCAAGAGGA 
AAGATATACC TAATCCTCGA TGCTGTTGAT GGGAAACCAA ATCAAAGATT GCTGCAACAC 
CAGTTGGACC AACACTTCTG GAAGTTCTTT AGCAAGTCCA ATGGCGTCGC CTCTGTAAAC 
AGGAACATGA TTCCCGATTT CTTCGGTATT CCCGAATCCG TTGAATTGCT TTCCTTGGAA 
CCCTACTCAA AGGACCAAAT ATTAATACGA CTGGAGAACT TTAATACCGA AGGAAACGTA 
GTTAGCTTCA ACATTTACCC ACTTTTTGAG TCCCTAGATG GATATCAAAT TTGGGAAACC 
ACTCTGGATG GTAATATGTT GTTGGAGGAC GTGAAGAGAT TTAAATTTGC CCAAGATGGC 
ACCGGTTCCA TTCCTTCCTC CGTTGAATAT TACCATGCTC CTCACAATCC CTTGACGGCC 
AATTCAACGA TGAACGCCTC CGGATTCGTG GTCACACTGG TTCCCATGCA GATTCGCACA 
TTTATAATCC AACAAAAGTA TATTCCATCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609407.1
CDS	251..3103
Translation	MINVHLVPHSHDDVGWLKTVDQYYYGSQNKIQHAGVQYILDTVVEELLKDSSRRFIQVETFFFAKWYSEQTETVQRAVKKLVAQGRLEFAGGAWSMNDEATVHYQSVVDQFNLGLRYLKDTFGDCGRPTVGWQIDPFGHSREMASIFAQMAFNGEFFARMDYVDKKQRMLDLEMEMIWQSSESLKNSNIFTGMLYNHYSAPPGFCFDINCEDAPIIDGESYDNNVDARVSEFIDYVKNMSKSYRSTHIMVPMGDDFQYEDAAVNFKNMDKLIKYVNDRQSTGSQVNVFYSTPSCYLYELHQLKQTWPNKTEDFFPYSSDSHSYWTGYFTSRPTQKRFHRDGNHFFQTVKQLSVLANLSGTQYSEDLDNLSQAMGIMQHHDAVTGTEKQAVASDYDRLLFKAIVGAENSARDALRSLTNLTSGEFESCLELNISVCAFTQDTANNVIVTLVNPLAHSSTQYVRVPAKNENYIVTDEKGREVFSEVVPVPWQVLALEHRPNDTQHELVFEASVDKIANFLIRVLPSPKNIAEDQVQFPVERSQDELTVETSLVKLTFDTTTGGLKTVKMNGFTENIQQTFGIYKGYRGNNGESKNRSSGAYVFRPYGDIEIVNNKVELSFYNGTKVKEVHQHVNEWISQVIRIYEDVNRVEFEWLVGPIPTDDDVGKEIITRFSSNISSKGKFYTDSNGREILERERNQREHFTPDMSEAISGNYYPVTGQISLQDDEKRITLLNDRAQGGTSLKDGELELMLHRRLLNDDAFGVGEALNETQYGTGLIARGKIYLILDAVDGKPNQRLLQHQLDQHFWKFFSKSNGVASVNRNMIPDFFGIPESVELLSLEPYSKDQILIRLENFNTEGNVVSFNIYPLFESLDGYQIWETTLDGNMLLEDVKRFKFAQDGTGSIPSSVEYYHAPHNPLTANSTMNASGFVVTLVPMQIRTFIIQQKYIPS

Target ORF information:

RefSeq Version	NM_135563.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lysosomal alpha-mannosidase I, transcript variant A (LManI), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135563.3

ORF Insert Sequence:

ATGATCAATG TGCACTTGGT GCCGCATTCG CACGACGACG TTGGATGGCT AAAGACAGTG 
GATCAGTATT ATTATGGTAG TCAGAACAAG ATACAACACG CTGGAGTCCA ATACATTTTG 
GACACCGTGG TGGAGGAGCT CCTCAAGGAT TCGTCGAGGC GCTTCATCCA GGTGGAAACG 
TTTTTCTTTG CAAAATGGTA TTCGGAGCAA ACTGAAACGG TCCAAAGGGC GGTGAAAAAG 
TTGGTGGCCC AAGGACGTTT GGAATTCGCT GGTGGAGCTT GGAGCATGAA CGATGAGGCC 
ACAGTTCACT ATCAAAGTGT TGTAGATCAG TTTAACCTGG GCTTAAGATA TCTTAAAGAT 
ACTTTTGGTG ATTGTGGACG CCCCACGGTT GGTTGGCAGA TCGATCCATT TGGTCATTCC 
CGAGAAATGG CCTCCATATT CGCACAAATG GCATTTAATG GTGAATTTTT CGCACGCATG 
GACTACGTTG ATAAGAAGCA ACGTATGCTG GATCTGGAAA TGGAGATGAT TTGGCAGTCC 
AGCGAGTCCC TAAAGAATTC GAATATATTC ACTGGCATGC TCTACAATCA CTATTCAGCT 
CCTCCGGGAT TCTGTTTCGA TATCAATTGC GAGGATGCAC CAATTATCGA TGGCGAGAGC 
TATGATAATA ACGTGGATGC GAGAGTCAGT GAGTTTATAG ACTATGTTAA GAACATGTCT 
AAGTCGTATC GATCCACTCA CATCATGGTG CCCATGGGAG ATGATTTTCA GTACGAAGAT 
GCAGCAGTTA ACTTCAAGAA TATGGACAAA CTTATCAAGT ACGTCAACGA TCGCCAATCG 
ACGGGATCCC AAGTCAACGT ATTCTACTCC ACACCCTCGT GTTATCTTTA TGAATTGCAC 
CAATTGAAGC AAACTTGGCC AAACAAAACG GAAGACTTCT TTCCATATTC CAGTGATTCG 
CACTCCTACT GGACCGGATA CTTCACCTCA CGACCCACCC AAAAACGTTT CCACAGGGAT 
GGCAATCACT TCTTCCAAAC TGTTAAGCAA TTGAGTGTGT TGGCCAACTT GAGTGGCACC 
CAGTACTCCG AAGATCTGGA TAATCTGAGT CAGGCAATGG GCATAATGCA GCATCATGAT 
GCTGTTACGG GTACCGAAAA ACAGGCGGTG GCCAGCGACT ATGATCGCTT ATTGTTCAAG 
GCCATCGTTG GAGCTGAAAA CAGTGCCCGA GATGCCCTAC GATCTCTGAC CAATCTGACC 
TCAGGGGAGT TCGAGAGTTG CCTGGAACTC AACATAAGTG TTTGTGCCTT CACCCAAGAT 
ACCGCCAACA ATGTGATTGT GACCCTGGTC AATCCATTGG CTCATTCTTC AACACAATAT 
GTGCGTGTTC CGGCGAAGAA CGAGAACTAC ATAGTCACCG ATGAGAAAGG TCGTGAAGTA 
TTTTCCGAAG TGGTTCCCGT TCCCTGGCAA GTTTTGGCGT TGGAACATCG ACCTAATGAT 
ACCCAGCACG AGTTGGTTTT TGAAGCCAGT GTTGATAAAA TAGCCAACTT TTTGATTCGT 
GTGCTACCAT CACCGAAAAA TATTGCAGAA GATCAAGTCC AATTCCCGGT AGAGAGGAGC 
CAGGATGAGT TAACAGTGGA GACTTCGCTG GTAAAACTGA CTTTTGATAC CACAACGGGT 
GGCTTGAAAA CTGTGAAAAT GAACGGATTT ACGGAAAATA TTCAACAGAC CTTTGGTATA 
TACAAGGGTT ATCGCGGCAA TAATGGTGAA TCGAAAAACC GATCATCCGG TGCCTATGTT 
TTCCGTCCAT ATGGCGATAT TGAAATTGTG AACAACAAGG TGGAGCTATC ATTCTATAAT 
GGCACCAAGG TTAAGGAAGT CCATCAGCAT GTCAACGAAT GGATCTCCCA GGTAATCCGA 
ATCTATGAGG ACGTCAATCG TGTTGAATTT GAATGGCTAG TCGGACCGAT TCCAACGGAT 
GATGATGTTG GCAAGGAGAT AATCACCCGG TTTTCCAGCA ACATATCCTC GAAGGGCAAG 
TTCTACACGG ACTCAAATGG TCGTGAAATC CTGGAACGTG AGAGAAATCA GCGGGAGCAC 
TTCACTCCGG ATATGTCGGA GGCTATAAGT GGAAACTATT ACCCGGTGAC TGGTCAAATT 
TCGTTACAAG ATGACGAAAA GCGAATCACC CTGCTGAATG ATAGAGCCCA GGGTGGAACC 
AGCCTAAAGG ATGGCGAACT GGAGCTGATG CTCCATCGTC GCCTTCTGAA CGATGATGCG 
TTTGGAGTGG GCGAAGCCCT AAATGAGACG CAATATGGAA CTGGTTTGAT TGCAAGAGGA 
AAGATATACC TAATCCTCGA TGCTGTTGAT GGGAAACCAA ATCAAAGATT GCTGCAACAC 
CAGTTGGACC AACACTTCTG GAAGTTCTTT AGCAAGTCCA ATGGCGTCGC CTCTGTAAAC 
AGGAACATGA TTCCCGATTT CTTCGGTATT CCCGAATCCG TTGAATTGCT TTCCTTGGAA 
CCCTACTCAA AGGACCAAAT ATTAATACGA CTGGAGAACT TTAATACCGA AGGAAACGTA 
GTTAGCTTCA ACATTTACCC ACTTTTTGAG TCCCTAGATG GATATCAAAT TTGGGAAACC 
ACTCTGGATG GTAATATGTT GTTGGAGGAC GTGAAGAGAT TTAAATTTGC CCAAGATGGC 
ACCGGTTCCA TTCCTTCCTC CGTTGAATAT TACCATGCTC CTCACAATCC CTTGACGGCC 
AATTCAACGA TGAACGCCTC CGGATTCGTG GTCACACTGG TTCCCATGCA GATTCGCACA 
TTTATAATCC AACAAAAGTA TATTCCATCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LManI ( NM_001273421.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260350.1 Drosophila melanogaster lysosomal alpha-mannosidase I, transcript variant B (LManI), mRNA.

Price & Availability↑

CloneID	OFa14361
Clone ID Related Accession (Same CDS sequence)	NM_135563.3 , NM_001273421.1
Accession Version	NM_001273421.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	lysosomal alpha-mannosidase I, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATCAATG TGCACTTGGT GCCGCATTCG CACGACGACG TTGGATGGCT AAAGACAGTG 
GATCAGTATT ATTATGGTAG TCAGAACAAG ATACAACACG CTGGAGTCCA ATACATTTTG 
GACACCGTGG TGGAGGAGCT CCTCAAGGAT TCGTCGAGGC GCTTCATCCA GGTGGAAACG 
TTTTTCTTTG CAAAATGGTA TTCGGAGCAA ACTGAAACGG TCCAAAGGGC GGTGAAAAAG 
TTGGTGGCCC AAGGACGTTT GGAATTCGCT GGTGGAGCTT GGAGCATGAA CGATGAGGCC 
ACAGTTCACT ATCAAAGTGT TGTAGATCAG TTTAACCTGG GCTTAAGATA TCTTAAAGAT 
ACTTTTGGTG ATTGTGGACG CCCCACGGTT GGTTGGCAGA TCGATCCATT TGGTCATTCC 
CGAGAAATGG CCTCCATATT CGCACAAATG GCATTTAATG GTGAATTTTT CGCACGCATG 
GACTACGTTG ATAAGAAGCA ACGTATGCTG GATCTGGAAA TGGAGATGAT TTGGCAGTCC 
AGCGAGTCCC TAAAGAATTC GAATATATTC ACTGGCATGC TCTACAATCA CTATTCAGCT 
CCTCCGGGAT TCTGTTTCGA TATCAATTGC GAGGATGCAC CAATTATCGA TGGCGAGAGC 
TATGATAATA ACGTGGATGC GAGAGTCAGT GAGTTTATAG ACTATGTTAA GAACATGTCT 
AAGTCGTATC GATCCACTCA CATCATGGTG CCCATGGGAG ATGATTTTCA GTACGAAGAT 
GCAGCAGTTA ACTTCAAGAA TATGGACAAA CTTATCAAGT ACGTCAACGA TCGCCAATCG 
ACGGGATCCC AAGTCAACGT ATTCTACTCC ACACCCTCGT GTTATCTTTA TGAATTGCAC 
CAATTGAAGC AAACTTGGCC AAACAAAACG GAAGACTTCT TTCCATATTC CAGTGATTCG 
CACTCCTACT GGACCGGATA CTTCACCTCA CGACCCACCC AAAAACGTTT CCACAGGGAT 
GGCAATCACT TCTTCCAAAC TGTTAAGCAA TTGAGTGTGT TGGCCAACTT GAGTGGCACC 
CAGTACTCCG AAGATCTGGA TAATCTGAGT CAGGCAATGG GCATAATGCA GCATCATGAT 
GCTGTTACGG GTACCGAAAA ACAGGCGGTG GCCAGCGACT ATGATCGCTT ATTGTTCAAG 
GCCATCGTTG GAGCTGAAAA CAGTGCCCGA GATGCCCTAC GATCTCTGAC CAATCTGACC 
TCAGGGGAGT TCGAGAGTTG CCTGGAACTC AACATAAGTG TTTGTGCCTT CACCCAAGAT 
ACCGCCAACA ATGTGATTGT GACCCTGGTC AATCCATTGG CTCATTCTTC AACACAATAT 
GTGCGTGTTC CGGCGAAGAA CGAGAACTAC ATAGTCACCG ATGAGAAAGG TCGTGAAGTA 
TTTTCCGAAG TGGTTCCCGT TCCCTGGCAA GTTTTGGCGT TGGAACATCG ACCTAATGAT 
ACCCAGCACG AGTTGGTTTT TGAAGCCAGT GTTGATAAAA TAGCCAACTT TTTGATTCGT 
GTGCTACCAT CACCGAAAAA TATTGCAGAA GATCAAGTCC AATTCCCGGT AGAGAGGAGC 
CAGGATGAGT TAACAGTGGA GACTTCGCTG GTAAAACTGA CTTTTGATAC CACAACGGGT 
GGCTTGAAAA CTGTGAAAAT GAACGGATTT ACGGAAAATA TTCAACAGAC CTTTGGTATA 
TACAAGGGTT ATCGCGGCAA TAATGGTGAA TCGAAAAACC GATCATCCGG TGCCTATGTT 
TTCCGTCCAT ATGGCGATAT TGAAATTGTG AACAACAAGG TGGAGCTATC ATTCTATAAT 
GGCACCAAGG TTAAGGAAGT CCATCAGCAT GTCAACGAAT GGATCTCCCA GGTAATCCGA 
ATCTATGAGG ACGTCAATCG TGTTGAATTT GAATGGCTAG TCGGACCGAT TCCAACGGAT 
GATGATGTTG GCAAGGAGAT AATCACCCGG TTTTCCAGCA ACATATCCTC GAAGGGCAAG 
TTCTACACGG ACTCAAATGG TCGTGAAATC CTGGAACGTG AGAGAAATCA GCGGGAGCAC 
TTCACTCCGG ATATGTCGGA GGCTATAAGT GGAAACTATT ACCCGGTGAC TGGTCAAATT 
TCGTTACAAG ATGACGAAAA GCGAATCACC CTGCTGAATG ATAGAGCCCA GGGTGGAACC 
AGCCTAAAGG ATGGCGAACT GGAGCTGATG CTCCATCGTC GCCTTCTGAA CGATGATGCG 
TTTGGAGTGG GCGAAGCCCT AAATGAGACG CAATATGGAA CTGGTTTGAT TGCAAGAGGA 
AAGATATACC TAATCCTCGA TGCTGTTGAT GGGAAACCAA ATCAAAGATT GCTGCAACAC 
CAGTTGGACC AACACTTCTG GAAGTTCTTT AGCAAGTCCA ATGGCGTCGC CTCTGTAAAC 
AGGAACATGA TTCCCGATTT CTTCGGTATT CCCGAATCCG TTGAATTGCT TTCCTTGGAA 
CCCTACTCAA AGGACCAAAT ATTAATACGA CTGGAGAACT TTAATACCGA AGGAAACGTA 
GTTAGCTTCA ACATTTACCC ACTTTTTGAG TCCCTAGATG GATATCAAAT TTGGGAAACC 
ACTCTGGATG GTAATATGTT GTTGGAGGAC GTGAAGAGAT TTAAATTTGC CCAAGATGGC 
ACCGGTTCCA TTCCTTCCTC CGTTGAATAT TACCATGCTC CTCACAATCC CTTGACGGCC 
AATTCAACGA TGAACGCCTC CGGATTCGTG GTCACACTGG TTCCCATGCA GATTCGCACA 
TTTATAATCC AACAAAAGTA TATTCCATCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260350.1
CDS	363..3215
Translation	MINVHLVPHSHDDVGWLKTVDQYYYGSQNKIQHAGVQYILDTVVEELLKDSSRRFIQVETFFFAKWYSEQTETVQRAVKKLVAQGRLEFAGGAWSMNDEATVHYQSVVDQFNLGLRYLKDTFGDCGRPTVGWQIDPFGHSREMASIFAQMAFNGEFFARMDYVDKKQRMLDLEMEMIWQSSESLKNSNIFTGMLYNHYSAPPGFCFDINCEDAPIIDGESYDNNVDARVSEFIDYVKNMSKSYRSTHIMVPMGDDFQYEDAAVNFKNMDKLIKYVNDRQSTGSQVNVFYSTPSCYLYELHQLKQTWPNKTEDFFPYSSDSHSYWTGYFTSRPTQKRFHRDGNHFFQTVKQLSVLANLSGTQYSEDLDNLSQAMGIMQHHDAVTGTEKQAVASDYDRLLFKAIVGAENSARDALRSLTNLTSGEFESCLELNISVCAFTQDTANNVIVTLVNPLAHSSTQYVRVPAKNENYIVTDEKGREVFSEVVPVPWQVLALEHRPNDTQHELVFEASVDKIANFLIRVLPSPKNIAEDQVQFPVERSQDELTVETSLVKLTFDTTTGGLKTVKMNGFTENIQQTFGIYKGYRGNNGESKNRSSGAYVFRPYGDIEIVNNKVELSFYNGTKVKEVHQHVNEWISQVIRIYEDVNRVEFEWLVGPIPTDDDVGKEIITRFSSNISSKGKFYTDSNGREILERERNQREHFTPDMSEAISGNYYPVTGQISLQDDEKRITLLNDRAQGGTSLKDGELELMLHRRLLNDDAFGVGEALNETQYGTGLIARGKIYLILDAVDGKPNQRLLQHQLDQHFWKFFSKSNGVASVNRNMIPDFFGIPESVELLSLEPYSKDQILIRLENFNTEGNVVSFNIYPLFESLDGYQIWETTLDGNMLLEDVKRFKFAQDGTGSIPSSVEYYHAPHNPLTANSTMNASGFVVTLVPMQIRTFIIQQKYIPS

Target ORF information:

RefSeq Version	NM_001273421.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster lysosomal alpha-mannosidase I, transcript variant B (LManI), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273421.1

ORF Insert Sequence:

ATGATCAATG TGCACTTGGT GCCGCATTCG CACGACGACG TTGGATGGCT AAAGACAGTG 
GATCAGTATT ATTATGGTAG TCAGAACAAG ATACAACACG CTGGAGTCCA ATACATTTTG 
GACACCGTGG TGGAGGAGCT CCTCAAGGAT TCGTCGAGGC GCTTCATCCA GGTGGAAACG 
TTTTTCTTTG CAAAATGGTA TTCGGAGCAA ACTGAAACGG TCCAAAGGGC GGTGAAAAAG 
TTGGTGGCCC AAGGACGTTT GGAATTCGCT GGTGGAGCTT GGAGCATGAA CGATGAGGCC 
ACAGTTCACT ATCAAAGTGT TGTAGATCAG TTTAACCTGG GCTTAAGATA TCTTAAAGAT 
ACTTTTGGTG ATTGTGGACG CCCCACGGTT GGTTGGCAGA TCGATCCATT TGGTCATTCC 
CGAGAAATGG CCTCCATATT CGCACAAATG GCATTTAATG GTGAATTTTT CGCACGCATG 
GACTACGTTG ATAAGAAGCA ACGTATGCTG GATCTGGAAA TGGAGATGAT TTGGCAGTCC 
AGCGAGTCCC TAAAGAATTC GAATATATTC ACTGGCATGC TCTACAATCA CTATTCAGCT 
CCTCCGGGAT TCTGTTTCGA TATCAATTGC GAGGATGCAC CAATTATCGA TGGCGAGAGC 
TATGATAATA ACGTGGATGC GAGAGTCAGT GAGTTTATAG ACTATGTTAA GAACATGTCT 
AAGTCGTATC GATCCACTCA CATCATGGTG CCCATGGGAG ATGATTTTCA GTACGAAGAT 
GCAGCAGTTA ACTTCAAGAA TATGGACAAA CTTATCAAGT ACGTCAACGA TCGCCAATCG 
ACGGGATCCC AAGTCAACGT ATTCTACTCC ACACCCTCGT GTTATCTTTA TGAATTGCAC 
CAATTGAAGC AAACTTGGCC AAACAAAACG GAAGACTTCT TTCCATATTC CAGTGATTCG 
CACTCCTACT GGACCGGATA CTTCACCTCA CGACCCACCC AAAAACGTTT CCACAGGGAT 
GGCAATCACT TCTTCCAAAC TGTTAAGCAA TTGAGTGTGT TGGCCAACTT GAGTGGCACC 
CAGTACTCCG AAGATCTGGA TAATCTGAGT CAGGCAATGG GCATAATGCA GCATCATGAT 
GCTGTTACGG GTACCGAAAA ACAGGCGGTG GCCAGCGACT ATGATCGCTT ATTGTTCAAG 
GCCATCGTTG GAGCTGAAAA CAGTGCCCGA GATGCCCTAC GATCTCTGAC CAATCTGACC 
TCAGGGGAGT TCGAGAGTTG CCTGGAACTC AACATAAGTG TTTGTGCCTT CACCCAAGAT 
ACCGCCAACA ATGTGATTGT GACCCTGGTC AATCCATTGG CTCATTCTTC AACACAATAT 
GTGCGTGTTC CGGCGAAGAA CGAGAACTAC ATAGTCACCG ATGAGAAAGG TCGTGAAGTA 
TTTTCCGAAG TGGTTCCCGT TCCCTGGCAA GTTTTGGCGT TGGAACATCG ACCTAATGAT 
ACCCAGCACG AGTTGGTTTT TGAAGCCAGT GTTGATAAAA TAGCCAACTT TTTGATTCGT 
GTGCTACCAT CACCGAAAAA TATTGCAGAA GATCAAGTCC AATTCCCGGT AGAGAGGAGC 
CAGGATGAGT TAACAGTGGA GACTTCGCTG GTAAAACTGA CTTTTGATAC CACAACGGGT 
GGCTTGAAAA CTGTGAAAAT GAACGGATTT ACGGAAAATA TTCAACAGAC CTTTGGTATA 
TACAAGGGTT ATCGCGGCAA TAATGGTGAA TCGAAAAACC GATCATCCGG TGCCTATGTT 
TTCCGTCCAT ATGGCGATAT TGAAATTGTG AACAACAAGG TGGAGCTATC ATTCTATAAT 
GGCACCAAGG TTAAGGAAGT CCATCAGCAT GTCAACGAAT GGATCTCCCA GGTAATCCGA 
ATCTATGAGG ACGTCAATCG TGTTGAATTT GAATGGCTAG TCGGACCGAT TCCAACGGAT 
GATGATGTTG GCAAGGAGAT AATCACCCGG TTTTCCAGCA ACATATCCTC GAAGGGCAAG 
TTCTACACGG ACTCAAATGG TCGTGAAATC CTGGAACGTG AGAGAAATCA GCGGGAGCAC 
TTCACTCCGG ATATGTCGGA GGCTATAAGT GGAAACTATT ACCCGGTGAC TGGTCAAATT 
TCGTTACAAG ATGACGAAAA GCGAATCACC CTGCTGAATG ATAGAGCCCA GGGTGGAACC 
AGCCTAAAGG ATGGCGAACT GGAGCTGATG CTCCATCGTC GCCTTCTGAA CGATGATGCG 
TTTGGAGTGG GCGAAGCCCT AAATGAGACG CAATATGGAA CTGGTTTGAT TGCAAGAGGA 
AAGATATACC TAATCCTCGA TGCTGTTGAT GGGAAACCAA ATCAAAGATT GCTGCAACAC 
CAGTTGGACC AACACTTCTG GAAGTTCTTT AGCAAGTCCA ATGGCGTCGC CTCTGTAAAC 
AGGAACATGA TTCCCGATTT CTTCGGTATT CCCGAATCCG TTGAATTGCT TTCCTTGGAA 
CCCTACTCAA AGGACCAAAT ATTAATACGA CTGGAGAACT TTAATACCGA AGGAAACGTA 
GTTAGCTTCA ACATTTACCC ACTTTTTGAG TCCCTAGATG GATATCAAAT TTGGGAAACC 
ACTCTGGATG GTAATATGTT GTTGGAGGAC GTGAAGAGAT TTAAATTTGC CCAAGATGGC 
ACCGGTTCCA TTCCTTCCTC CGTTGAATAT TACCATGCTC CTCACAATCC CTTGACGGCC 
AATTCAACGA TGAACGCCTC CGGATTCGTG GTCACACTGG TTCCCATGCA GATTCGCACA 
TTTATAATCC AACAAAAGTA TATTCCATCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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LManI cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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