Rsf1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rsf1
Entrez Gene ID	34370
Full Name	Repressor splicing factor 1
Synonyms	CG5655,Dmel\CG5655,ROX21,RSF1,Rox21,dRSF1,rox21
General protein information	Preferred Names Repressor splicing factor 1 Names CG5655 gene product from transcript CG5655-RB CG5655-PA CG5655-PB Rsf1-PA Rsf1-PB repressor splicing factor 1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 31B1-31B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001273389.1	NP_001260318.1	repressor splicing factor 1, isoform B
NM_057653.3	NP_477001.2	repressor splicing factor 1, isoform A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In Rsf-1 mutant, the levels of histone H3K9 dimethylation and histone H2A variant H2Av were significantly reduced in an euchromatic region juxtaposed with heterochromatin.
Title: RSF governs silent chromatin formation via histone H2Av replacement.

The following Rsf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rsf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14295	NM_001273389.1 Latest version!	Drosophila melanogaster repressor splicing factor 1, transcript variant B (Rsf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa14295	NM_057653.3 Latest version!	Drosophila melanogaster repressor splicing factor 1, transcript variant A (Rsf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rsf1 ( NM_001273389.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260318.1 Drosophila melanogaster repressor splicing factor 1, transcript variant B (Rsf1), mRNA.

Price & Availability↑

CloneID	OFa14295
Clone ID Related Accession (Same CDS sequence)	NM_001273389.1 , NM_057653.3
Accession Version	NM_001273389.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	repressor splicing factor 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAGCA TGGGTGATCA GCGCGGGACA CGGGTGTATG TCGGCAATCT GACCGACAAA 
GTGAAAAAGG ATGATCTGGA GGGGGAGTTC ACAAAGTATG GCAAGCTGAA TTCGGTGTGG 
ATAGCCTTCA ATCCGCCGGG ATTTGCGTTC GTCGAGTTCG AGCACCGCGA CGACGCCGAA 
AAGGCGTGCG ACATACTGAA CGGATCCGAG CTGCTCGGCT CCCAGCTGCG CGTGGAGATC 
TCAAAAGGGC GGCCACGCCA GGGTAGGCGT GGCGGACCCA TGGACAGGGG CGGACGACGC 
GGCGACTTTG GCCGGCACAG CATCACAAGC GGTGGTAGCG GCGGAGGCGG TTTCCGGCAG 
CGCGGATCCA GCGGATCCTC AAGCCGGCAC ACGGAGCGGG GCTATAGCTC CGGCCGATCA 
GGTGCAAGCA GCTATAATGG CAGAGAGGGC GGCGGCAGCG GCTTCAATCG CCGCGAGGTT 
TACGGCGGTG GACGCGACAG CAGCCGCTAC AGCAGCGGAA GTAGCGCCAG CTACGGACGC 
ACTGGTGGTC AGTCGGCCGG ACGCTTCAGG TCCCGCTCGC CGGTGGGAAA CCATCGATTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260318.1
CDS	137..739
Translation	MSSMGDQRGTRVYVGNLTDKVKKDDLEGEFTKYGKLNSVWIAFNPPGFAFVEFEHRDDAEKACDILNGSELLGSQLRVEISKGRPRQGRRGGPMDRGGRRGDFGRHSITSGGSGGGGFRQRGSSGSSSRHTERGYSSGRSGASSYNGREGGGSGFNRREVYGGGRDSSRYSSGSSASYGRTGGQSAGRFRSRSPVGNHRF

Target ORF information:

RefSeq Version	NM_001273389.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster repressor splicing factor 1, transcript variant B (Rsf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273389.1

ORF Insert Sequence:

ATGTCCAGCA TGGGTGATCA GCGCGGGACA CGGGTGTATG TCGGCAATCT GACCGACAAA 
GTGAAAAAGG ATGATCTGGA GGGGGAGTTC ACAAAGTATG GCAAGCTGAA TTCGGTGTGG 
ATAGCCTTCA ATCCGCCGGG ATTTGCGTTC GTCGAGTTCG AGCACCGCGA CGACGCCGAA 
AAGGCGTGCG ACATACTGAA CGGATCCGAG CTGCTCGGCT CCCAGCTGCG CGTGGAGATC 
TCAAAAGGGC GGCCACGCCA GGGTAGGCGT GGCGGACCCA TGGACAGGGG CGGACGACGC 
GGCGACTTTG GCCGGCACAG CATCACAAGC GGTGGTAGCG GCGGAGGCGG TTTCCGGCAG 
CGCGGATCCA GCGGATCCTC AAGCCGGCAC ACGGAGCGGG GCTATAGCTC CGGCCGATCA 
GGTGCAAGCA GCTATAATGG CAGAGAGGGC GGCGGCAGCG GCTTCAATCG CCGCGAGGTT 
TACGGCGGTG GACGCGACAG CAGCCGCTAC AGCAGCGGAA GTAGCGCCAG CTACGGACGC 
ACTGGTGGTC AGTCGGCCGG ACGCTTCAGG TCCCGCTCGC CGGTGGGAAA CCATCGATTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rsf1 ( NM_057653.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477001.2 Drosophila melanogaster repressor splicing factor 1, transcript variant A (Rsf1), mRNA.

Price & Availability↑

CloneID	OFa14295
Clone ID Related Accession (Same CDS sequence)	NM_001273389.1 , NM_057653.3
Accession Version	NM_057653.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	repressor splicing factor 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057653.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCCAGCA TGGGTGATCA GCGCGGGACA CGGGTGTATG TCGGCAATCT GACCGACAAA 
GTGAAAAAGG ATGATCTGGA GGGGGAGTTC ACAAAGTATG GCAAGCTGAA TTCGGTGTGG 
ATAGCCTTCA ATCCGCCGGG ATTTGCGTTC GTCGAGTTCG AGCACCGCGA CGACGCCGAA 
AAGGCGTGCG ACATACTGAA CGGATCCGAG CTGCTCGGCT CCCAGCTGCG CGTGGAGATC 
TCAAAAGGGC GGCCACGCCA GGGTAGGCGT GGCGGACCCA TGGACAGGGG CGGACGACGC 
GGCGACTTTG GCCGGCACAG CATCACAAGC GGTGGTAGCG GCGGAGGCGG TTTCCGGCAG 
CGCGGATCCA GCGGATCCTC AAGCCGGCAC ACGGAGCGGG GCTATAGCTC CGGCCGATCA 
GGTGCAAGCA GCTATAATGG CAGAGAGGGC GGCGGCAGCG GCTTCAATCG CCGCGAGGTT 
TACGGCGGTG GACGCGACAG CAGCCGCTAC AGCAGCGGAA GTAGCGCCAG CTACGGACGC 
ACTGGTGGTC AGTCGGCCGG ACGCTTCAGG TCCCGCTCGC CGGTGGGAAA CCATCGATTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477001.2
CDS	137..739
Translation	MSSMGDQRGTRVYVGNLTDKVKKDDLEGEFTKYGKLNSVWIAFNPPGFAFVEFEHRDDAEKACDILNGSELLGSQLRVEISKGRPRQGRRGGPMDRGGRRGDFGRHSITSGGSGGGGFRQRGSSGSSSRHTERGYSSGRSGASSYNGREGGGSGFNRREVYGGGRDSSRYSSGSSASYGRTGGQSAGRFRSRSPVGNHRF

Target ORF information:

RefSeq Version	NM_057653.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster repressor splicing factor 1, transcript variant A (Rsf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057653.3

ORF Insert Sequence:

ATGTCCAGCA TGGGTGATCA GCGCGGGACA CGGGTGTATG TCGGCAATCT GACCGACAAA 
GTGAAAAAGG ATGATCTGGA GGGGGAGTTC ACAAAGTATG GCAAGCTGAA TTCGGTGTGG 
ATAGCCTTCA ATCCGCCGGG ATTTGCGTTC GTCGAGTTCG AGCACCGCGA CGACGCCGAA 
AAGGCGTGCG ACATACTGAA CGGATCCGAG CTGCTCGGCT CCCAGCTGCG CGTGGAGATC 
TCAAAAGGGC GGCCACGCCA GGGTAGGCGT GGCGGACCCA TGGACAGGGG CGGACGACGC 
GGCGACTTTG GCCGGCACAG CATCACAAGC GGTGGTAGCG GCGGAGGCGG TTTCCGGCAG 
CGCGGATCCA GCGGATCCTC AAGCCGGCAC ACGGAGCGGG GCTATAGCTC CGGCCGATCA 
GGTGCAAGCA GCTATAATGG CAGAGAGGGC GGCGGCAGCG GCTTCAATCG CCGCGAGGTT 
TACGGCGGTG GACGCGACAG CAGCCGCTAC AGCAGCGGAA GTAGCGCCAG CTACGGACGC 
ACTGGTGGTC AGTCGGCCGG ACGCTTCAGG TCCCGCTCGC CGGTGGGAAA CCATCGATTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Rsf1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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