Dref cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dref
Entrez Gene ID	34328
Full Name	DNA replication-related element factor
Synonyms	CG5838,DREF,DmDREF,Dmel\CG5838,Dmel_DREF,dDREF,dref,p80,p80/DREF
General protein information	Preferred Names DNA replication-related element factor Names CG5838 gene product from transcript CG5838-RA CG5838-PA CG5838-PB DNA DNA replication-related element binding factor DNA replication-related element-binding factor DNA-replication-related element factor DRE (DNA replication-related element) binding factor DRE-binding factor Dref-PA Dref-PB replication-related element-binding factor
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 30F2-30F3

mRNA and Protein(s)

mRNA	Protein	Name
NM_078805.4	NP_523529.1	DNA replication-related element factor, isoform A
NM_001273382.2	NP_001260311.1	DNA replication-related element factor, isoform B

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	LOC100486384	XP_004912075.1
Drosophila melanogaster (fruit fly)	Dref	NP_001260311.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DREF is now emerging as not only a transcription factor, but also a multi-functional protein. In this review, we summarize current advances in studies on the novel functions of Drosophila DREF.
Title: DREF plays multiple roles during Drosophila development.

Together, these results indicate that the DRE/DREF pathway is required for transcriptional activation of the hpo gene to positively control Hippo pathways.
Title: The Hippo pathway as a target of the Drosophila DRE/DREF transcriptional regulatory pathway.

Results show that regulation of e2f1 and PCNA by DREF in vivo is complex and the regulation mechanism may differ with the tissue and/or positions in the tissue.
Title: Control of e2f1 and PCNA by Drosophila transcription factor DREF.

DREF proteins may help maintain chromosome organization during the cell cycle and mark a subset of genomic sites for the assembly of pre-replication complexes and gene bookmarking during the M/G1 transition.
Title: Dynamic changes in the genomic localization of DNA replication-related element binding factor during the cell cycle.

expression of the dref gene occurs in clusters and is temporally regulated at specific times during bristle development.
Title: Temporal and spatial pattern of dref expression during Drosophila bristle development.

The following Dref gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dref cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa14260	NM_001273382.2 Latest version!	Drosophila melanogaster DNA replication-related element factor (Dref), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa14260	NM_078805.4 Latest version!	Drosophila melanogaster DNA replication-related element factor (Dref), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dref ( NM_078805.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523529.1 Drosophila melanogaster DNA replication-related element factor (Dref), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa14260
Clone ID Related Accession (Same CDS sequence)	NM_078805.4 , NM_001273382.2
Accession Version	NM_078805.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	DNA replication-related element factor, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_078805.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGAAG GGGTACCAGC GTCGCCCGTG GCCATTGGCG AACTCAAGTA CGAGGATGTG 
TCGCAGCTAA ATTTTTCCAA ACTGTACTCG CCCAAGATGA AAAGCGTATA CTGGCGCTAC 
TTTGGATTCC CCTCGAACGA CAATAATGAG GTGATTACCA AACAGAATGT GGTCTGCATT 
AAGTGTCACA AGGTGCTGAC CAACCATGGC AACACCACCA ATTTGCGTGC GCATCTCCAG 
CACCGACACA AGGATCTGTT CAAGGAGCTG TGCCAGGAGC ACGACATCCA TGTGCCGCCG 
CGCAAGACGC CGCGTAATGT ATCCCATCCA CCGCTGTCCA AGCGGAATGT TTCCTCGCGG 
CGGGTCAAAC TGGAGTTCAT TAATAACCGG AACCACGATA ACGCTTCCGA TGATGAACTG 
GACGAAGCAG CCGTGGCAAC TGCGGCCATG CAGGCGGAGG AGGACGCCTC CTCGCAAACC 
ATGCTGTACG AGACCATGGT GCCCTTCACC TACGACGAGG CCGAAAATCT GGTAGAGGAG 
GAGGAGCGCC TAGTTATGGA ACCAAAGTAC GGACGCAAAC GCAAAGTGGC CACTCCATCG 
AGTGCCCTGA TGCACGGGCG TGTCATCAAG CACGAGGAAG GCGGCTATGC CGCCGTGGCG 
AACATCGCCA ATCTGGCCGA AGCCCTTACG GACATTGTGA TTAAGGATTT GCGCAATGTG 
GATTCTCTGT ACGATGCCGG TTTTGGTGAA TTCCTGCGTC AAGTCCTAGG CAATTCGGCA 
GCCATGCCCG AGCCACACAA GATCGACTCT TTGATCAATG AGATGCACGC CTCCAAGTTT 
CTGGAGATTG GCGAAATCAC GCGCGATTTC ACCTCCGAGA AGCCCTTCTC TCTCGCCTTT 
GAGATGTGGG TGAATGTTGA ACAGCGGCGC TTCCTTAGCA TCTTCCATCA CTTTCTGGAC 
GAGGAGACGC ACTCAGTTCG CGGTATGCTG TATGCCACCG TGGAGTACAA CGACTACATC 
GTCTTTGACG ATCTGCTGAC CGACTTCTAT TTGGCCAATT GCACACTGGC CATCATAAAC 
TACGACGAGG AAGAGGACCT TTTGCACACC TATCTGCGAG AGAAAAATAT ACCCATTTCG 
CTGTGCTACG TTTCGGTAAT TGACAAATGT TTGCGTCGTG TGTTTGAGAT CGAAGAGGTG 
GCCACTTTAT TGGAGCAGGT GAAGGACCTA ATGCAGCGTC ATTCAACGGA GATTGCCTCC 
AAGGTGTCCG AGGTGCCCAT GCCCACGTAC AACGAGCACT TCCCCTGGAC ACTGTACGAA 
ACATTGAAGT TCTTTGCCGA GTCCATATCT TGGTCCGAGG ACATGGATCA CCTGGTTATA 
TCAGCCAAGA CGGTGACGGA GGCCCTGAGT GCTCTGGTGA TTGCTTTGGA CACGCTGCGT 
GGCGAAGACA TTCCCTTGTG TAGCATGCTT TCGCCAATCA CTTCAAAGAT TCTTATCAAG 
AAGTTGGGCA TCGCCGAGCA GGACGATCCC CTAATGATGA ATTTGAAGCG GACCATTTCC 
AGTGTCCTCC AGGCGCACGT TATATCCAAT GACAATCTGA CCGCTGCTGC ATTGTTGGAC 
CCACGCTTCC ACCGCCTGAC CACCATTGAC AACCTAGAGC GAACCGTTCG TATGCTGACC 
CATAAGTATA ACATTAACTT TGGTGGAGTG GGCGAAGGGG AATCCAACGA AGTGGCAGCT 
ACCTCCAGCG TGGTGGCCAT TAAGTCAGAA CCAAGAGTAG TGGACGGCAG TGCACCAAAG 
AAGTTGGGTC TGAAACTGCT GTTTGACAGT AACGAGATAC CAAATCCTCC GAAGCGAGAT 
GCGGACAGCA GCGTGGAGTC CGATCTTAAG CGATATCGCA ACGAGGTGGT CGTTCAGCTG 
GATGAGTCGC CCATCGAGTG GTGGCTCAAG ATGGGACACA TTTATGGAAC GCTCCGCGAT 
TTGGCCAGCC TGTACCATAG TGTGCCCGGC GTGGTGACGC TCAGCTTCAA GAAGGCGCTG 
AGAGACCAAA TATACGACTT CAACAAGCGA TTCATGCTCA CCGGTAGTCA CATCGACGCC 
ATCCTCTTTC TTCATCATCA CAACAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523529.1
CDS	345..2474
Translation	MSEGVPASPVAIGELKYEDVSQLNFSKLYSPKMKSVYWRYFGFPSNDNNEVITKQNVVCIKCHKVLTNHGNTTNLRAHLQHRHKDLFKELCQEHDIHVPPRKTPRNVSHPPLSKRNVSSRRVKLEFINNRNHDNASDDELDEAAVATAAMQAEEDASSQTMLYETMVPFTYDEAENLVEEEERLVMEPKYGRKRKVATPSSALMHGRVIKHEEGGYAAVANIANLAEALTDIVIKDLRNVDSLYDAGFGEFLRQVLGNSAAMPEPHKIDSLINEMHASKFLEIGEITRDFTSEKPFSLAFEMWVNVEQRRFLSIFHHFLDEETHSVRGMLYATVEYNDYIVFDDLLTDFYLANCTLAIINYDEEEDLLHTYLREKNIPISLCYVSVIDKCLRRVFEIEEVATLLEQVKDLMQRHSTEIASKVSEVPMPTYNEHFPWTLYETLKFFAESISWSEDMDHLVISAKTVTEALSALVIALDTLRGEDIPLCSMLSPITSKILIKKLGIAEQDDPLMMNLKRTISSVLQAHVISNDNLTAAALLDPRFHRLTTIDNLERTVRMLTHKYNINFGGVGEGESNEVAATSSVVAIKSEPRVVDGSAPKKLGLKLLFDSNEIPNPPKRDADSSVESDLKRYRNEVVVQLDESPIEWWLKMGHIYGTLRDLASLYHSVPGVVTLSFKKALRDQIYDFNKRFMLTGSHIDAILFLHHHNN

Target ORF information:

RefSeq Version	NM_078805.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster DNA replication-related element factor (Dref), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078805.4

ORF Insert Sequence:

ATGAGCGAAG GGGTACCAGC GTCGCCCGTG GCCATTGGCG AACTCAAGTA CGAGGATGTG 
TCGCAGCTAA ATTTTTCCAA ACTGTACTCG CCCAAGATGA AAAGCGTATA CTGGCGCTAC 
TTTGGATTCC CCTCGAACGA CAATAATGAG GTGATTACCA AACAGAATGT GGTCTGCATT 
AAGTGTCACA AGGTGCTGAC CAACCATGGC AACACCACCA ATTTGCGTGC GCATCTCCAG 
CACCGACACA AGGATCTGTT CAAGGAGCTG TGCCAGGAGC ACGACATCCA TGTGCCGCCG 
CGCAAGACGC CGCGTAATGT ATCCCATCCA CCGCTGTCCA AGCGGAATGT TTCCTCGCGG 
CGGGTCAAAC TGGAGTTCAT TAATAACCGG AACCACGATA ACGCTTCCGA TGATGAACTG 
GACGAAGCAG CCGTGGCAAC TGCGGCCATG CAGGCGGAGG AGGACGCCTC CTCGCAAACC 
ATGCTGTACG AGACCATGGT GCCCTTCACC TACGACGAGG CCGAAAATCT GGTAGAGGAG 
GAGGAGCGCC TAGTTATGGA ACCAAAGTAC GGACGCAAAC GCAAAGTGGC CACTCCATCG 
AGTGCCCTGA TGCACGGGCG TGTCATCAAG CACGAGGAAG GCGGCTATGC CGCCGTGGCG 
AACATCGCCA ATCTGGCCGA AGCCCTTACG GACATTGTGA TTAAGGATTT GCGCAATGTG 
GATTCTCTGT ACGATGCCGG TTTTGGTGAA TTCCTGCGTC AAGTCCTAGG CAATTCGGCA 
GCCATGCCCG AGCCACACAA GATCGACTCT TTGATCAATG AGATGCACGC CTCCAAGTTT 
CTGGAGATTG GCGAAATCAC GCGCGATTTC ACCTCCGAGA AGCCCTTCTC TCTCGCCTTT 
GAGATGTGGG TGAATGTTGA ACAGCGGCGC TTCCTTAGCA TCTTCCATCA CTTTCTGGAC 
GAGGAGACGC ACTCAGTTCG CGGTATGCTG TATGCCACCG TGGAGTACAA CGACTACATC 
GTCTTTGACG ATCTGCTGAC CGACTTCTAT TTGGCCAATT GCACACTGGC CATCATAAAC 
TACGACGAGG AAGAGGACCT TTTGCACACC TATCTGCGAG AGAAAAATAT ACCCATTTCG 
CTGTGCTACG TTTCGGTAAT TGACAAATGT TTGCGTCGTG TGTTTGAGAT CGAAGAGGTG 
GCCACTTTAT TGGAGCAGGT GAAGGACCTA ATGCAGCGTC ATTCAACGGA GATTGCCTCC 
AAGGTGTCCG AGGTGCCCAT GCCCACGTAC AACGAGCACT TCCCCTGGAC ACTGTACGAA 
ACATTGAAGT TCTTTGCCGA GTCCATATCT TGGTCCGAGG ACATGGATCA CCTGGTTATA 
TCAGCCAAGA CGGTGACGGA GGCCCTGAGT GCTCTGGTGA TTGCTTTGGA CACGCTGCGT 
GGCGAAGACA TTCCCTTGTG TAGCATGCTT TCGCCAATCA CTTCAAAGAT TCTTATCAAG 
AAGTTGGGCA TCGCCGAGCA GGACGATCCC CTAATGATGA ATTTGAAGCG GACCATTTCC 
AGTGTCCTCC AGGCGCACGT TATATCCAAT GACAATCTGA CCGCTGCTGC ATTGTTGGAC 
CCACGCTTCC ACCGCCTGAC CACCATTGAC AACCTAGAGC GAACCGTTCG TATGCTGACC 
CATAAGTATA ACATTAACTT TGGTGGAGTG GGCGAAGGGG AATCCAACGA AGTGGCAGCT 
ACCTCCAGCG TGGTGGCCAT TAAGTCAGAA CCAAGAGTAG TGGACGGCAG TGCACCAAAG 
AAGTTGGGTC TGAAACTGCT GTTTGACAGT AACGAGATAC CAAATCCTCC GAAGCGAGAT 
GCGGACAGCA GCGTGGAGTC CGATCTTAAG CGATATCGCA ACGAGGTGGT CGTTCAGCTG 
GATGAGTCGC CCATCGAGTG GTGGCTCAAG ATGGGACACA TTTATGGAAC GCTCCGCGAT 
TTGGCCAGCC TGTACCATAG TGTGCCCGGC GTGGTGACGC TCAGCTTCAA GAAGGCGCTG 
AGAGACCAAA TATACGACTT CAACAAGCGA TTCATGCTCA CCGGTAGTCA CATCGACGCC 
ATCCTCTTTC TTCATCATCA CAACAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dref ( NM_001273382.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260311.1 Drosophila melanogaster DNA replication-related element factor (Dref), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa14260
Clone ID Related Accession (Same CDS sequence)	NM_078805.4 , NM_001273382.2
Accession Version	NM_001273382.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	DNA replication-related element factor, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001273382.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGAAG GGGTACCAGC GTCGCCCGTG GCCATTGGCG AACTCAAGTA CGAGGATGTG 
TCGCAGCTAA ATTTTTCCAA ACTGTACTCG CCCAAGATGA AAAGCGTATA CTGGCGCTAC 
TTTGGATTCC CCTCGAACGA CAATAATGAG GTGATTACCA AACAGAATGT GGTCTGCATT 
AAGTGTCACA AGGTGCTGAC CAACCATGGC AACACCACCA ATTTGCGTGC GCATCTCCAG 
CACCGACACA AGGATCTGTT CAAGGAGCTG TGCCAGGAGC ACGACATCCA TGTGCCGCCG 
CGCAAGACGC CGCGTAATGT ATCCCATCCA CCGCTGTCCA AGCGGAATGT TTCCTCGCGG 
CGGGTCAAAC TGGAGTTCAT TAATAACCGG AACCACGATA ACGCTTCCGA TGATGAACTG 
GACGAAGCAG CCGTGGCAAC TGCGGCCATG CAGGCGGAGG AGGACGCCTC CTCGCAAACC 
ATGCTGTACG AGACCATGGT GCCCTTCACC TACGACGAGG CCGAAAATCT GGTAGAGGAG 
GAGGAGCGCC TAGTTATGGA ACCAAAGTAC GGACGCAAAC GCAAAGTGGC CACTCCATCG 
AGTGCCCTGA TGCACGGGCG TGTCATCAAG CACGAGGAAG GCGGCTATGC CGCCGTGGCG 
AACATCGCCA ATCTGGCCGA AGCCCTTACG GACATTGTGA TTAAGGATTT GCGCAATGTG 
GATTCTCTGT ACGATGCCGG TTTTGGTGAA TTCCTGCGTC AAGTCCTAGG CAATTCGGCA 
GCCATGCCCG AGCCACACAA GATCGACTCT TTGATCAATG AGATGCACGC CTCCAAGTTT 
CTGGAGATTG GCGAAATCAC GCGCGATTTC ACCTCCGAGA AGCCCTTCTC TCTCGCCTTT 
GAGATGTGGG TGAATGTTGA ACAGCGGCGC TTCCTTAGCA TCTTCCATCA CTTTCTGGAC 
GAGGAGACGC ACTCAGTTCG CGGTATGCTG TATGCCACCG TGGAGTACAA CGACTACATC 
GTCTTTGACG ATCTGCTGAC CGACTTCTAT TTGGCCAATT GCACACTGGC CATCATAAAC 
TACGACGAGG AAGAGGACCT TTTGCACACC TATCTGCGAG AGAAAAATAT ACCCATTTCG 
CTGTGCTACG TTTCGGTAAT TGACAAATGT TTGCGTCGTG TGTTTGAGAT CGAAGAGGTG 
GCCACTTTAT TGGAGCAGGT GAAGGACCTA ATGCAGCGTC ATTCAACGGA GATTGCCTCC 
AAGGTGTCCG AGGTGCCCAT GCCCACGTAC AACGAGCACT TCCCCTGGAC ACTGTACGAA 
ACATTGAAGT TCTTTGCCGA GTCCATATCT TGGTCCGAGG ACATGGATCA CCTGGTTATA 
TCAGCCAAGA CGGTGACGGA GGCCCTGAGT GCTCTGGTGA TTGCTTTGGA CACGCTGCGT 
GGCGAAGACA TTCCCTTGTG TAGCATGCTT TCGCCAATCA CTTCAAAGAT TCTTATCAAG 
AAGTTGGGCA TCGCCGAGCA GGACGATCCC CTAATGATGA ATTTGAAGCG GACCATTTCC 
AGTGTCCTCC AGGCGCACGT TATATCCAAT GACAATCTGA CCGCTGCTGC ATTGTTGGAC 
CCACGCTTCC ACCGCCTGAC CACCATTGAC AACCTAGAGC GAACCGTTCG TATGCTGACC 
CATAAGTATA ACATTAACTT TGGTGGAGTG GGCGAAGGGG AATCCAACGA AGTGGCAGCT 
ACCTCCAGCG TGGTGGCCAT TAAGTCAGAA CCAAGAGTAG TGGACGGCAG TGCACCAAAG 
AAGTTGGGTC TGAAACTGCT GTTTGACAGT AACGAGATAC CAAATCCTCC GAAGCGAGAT 
GCGGACAGCA GCGTGGAGTC CGATCTTAAG CGATATCGCA ACGAGGTGGT CGTTCAGCTG 
GATGAGTCGC CCATCGAGTG GTGGCTCAAG ATGGGACACA TTTATGGAAC GCTCCGCGAT 
TTGGCCAGCC TGTACCATAG TGTGCCCGGC GTGGTGACGC TCAGCTTCAA GAAGGCGCTG 
AGAGACCAAA TATACGACTT CAACAAGCGA TTCATGCTCA CCGGTAGTCA CATCGACGCC 
ATCCTCTTTC TTCATCATCA CAACAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260311.1
CDS	345..2474
Translation	MSEGVPASPVAIGELKYEDVSQLNFSKLYSPKMKSVYWRYFGFPSNDNNEVITKQNVVCIKCHKVLTNHGNTTNLRAHLQHRHKDLFKELCQEHDIHVPPRKTPRNVSHPPLSKRNVSSRRVKLEFINNRNHDNASDDELDEAAVATAAMQAEEDASSQTMLYETMVPFTYDEAENLVEEEERLVMEPKYGRKRKVATPSSALMHGRVIKHEEGGYAAVANIANLAEALTDIVIKDLRNVDSLYDAGFGEFLRQVLGNSAAMPEPHKIDSLINEMHASKFLEIGEITRDFTSEKPFSLAFEMWVNVEQRRFLSIFHHFLDEETHSVRGMLYATVEYNDYIVFDDLLTDFYLANCTLAIINYDEEEDLLHTYLREKNIPISLCYVSVIDKCLRRVFEIEEVATLLEQVKDLMQRHSTEIASKVSEVPMPTYNEHFPWTLYETLKFFAESISWSEDMDHLVISAKTVTEALSALVIALDTLRGEDIPLCSMLSPITSKILIKKLGIAEQDDPLMMNLKRTISSVLQAHVISNDNLTAAALLDPRFHRLTTIDNLERTVRMLTHKYNINFGGVGEGESNEVAATSSVVAIKSEPRVVDGSAPKKLGLKLLFDSNEIPNPPKRDADSSVESDLKRYRNEVVVQLDESPIEWWLKMGHIYGTLRDLASLYHSVPGVVTLSFKKALRDQIYDFNKRFMLTGSHIDAILFLHHHNN

Target ORF information:

RefSeq Version	NM_001273382.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster DNA replication-related element factor (Dref), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273382.2

ORF Insert Sequence:

ATGAGCGAAG GGGTACCAGC GTCGCCCGTG GCCATTGGCG AACTCAAGTA CGAGGATGTG 
TCGCAGCTAA ATTTTTCCAA ACTGTACTCG CCCAAGATGA AAAGCGTATA CTGGCGCTAC 
TTTGGATTCC CCTCGAACGA CAATAATGAG GTGATTACCA AACAGAATGT GGTCTGCATT 
AAGTGTCACA AGGTGCTGAC CAACCATGGC AACACCACCA ATTTGCGTGC GCATCTCCAG 
CACCGACACA AGGATCTGTT CAAGGAGCTG TGCCAGGAGC ACGACATCCA TGTGCCGCCG 
CGCAAGACGC CGCGTAATGT ATCCCATCCA CCGCTGTCCA AGCGGAATGT TTCCTCGCGG 
CGGGTCAAAC TGGAGTTCAT TAATAACCGG AACCACGATA ACGCTTCCGA TGATGAACTG 
GACGAAGCAG CCGTGGCAAC TGCGGCCATG CAGGCGGAGG AGGACGCCTC CTCGCAAACC 
ATGCTGTACG AGACCATGGT GCCCTTCACC TACGACGAGG CCGAAAATCT GGTAGAGGAG 
GAGGAGCGCC TAGTTATGGA ACCAAAGTAC GGACGCAAAC GCAAAGTGGC CACTCCATCG 
AGTGCCCTGA TGCACGGGCG TGTCATCAAG CACGAGGAAG GCGGCTATGC CGCCGTGGCG 
AACATCGCCA ATCTGGCCGA AGCCCTTACG GACATTGTGA TTAAGGATTT GCGCAATGTG 
GATTCTCTGT ACGATGCCGG TTTTGGTGAA TTCCTGCGTC AAGTCCTAGG CAATTCGGCA 
GCCATGCCCG AGCCACACAA GATCGACTCT TTGATCAATG AGATGCACGC CTCCAAGTTT 
CTGGAGATTG GCGAAATCAC GCGCGATTTC ACCTCCGAGA AGCCCTTCTC TCTCGCCTTT 
GAGATGTGGG TGAATGTTGA ACAGCGGCGC TTCCTTAGCA TCTTCCATCA CTTTCTGGAC 
GAGGAGACGC ACTCAGTTCG CGGTATGCTG TATGCCACCG TGGAGTACAA CGACTACATC 
GTCTTTGACG ATCTGCTGAC CGACTTCTAT TTGGCCAATT GCACACTGGC CATCATAAAC 
TACGACGAGG AAGAGGACCT TTTGCACACC TATCTGCGAG AGAAAAATAT ACCCATTTCG 
CTGTGCTACG TTTCGGTAAT TGACAAATGT TTGCGTCGTG TGTTTGAGAT CGAAGAGGTG 
GCCACTTTAT TGGAGCAGGT GAAGGACCTA ATGCAGCGTC ATTCAACGGA GATTGCCTCC 
AAGGTGTCCG AGGTGCCCAT GCCCACGTAC AACGAGCACT TCCCCTGGAC ACTGTACGAA 
ACATTGAAGT TCTTTGCCGA GTCCATATCT TGGTCCGAGG ACATGGATCA CCTGGTTATA 
TCAGCCAAGA CGGTGACGGA GGCCCTGAGT GCTCTGGTGA TTGCTTTGGA CACGCTGCGT 
GGCGAAGACA TTCCCTTGTG TAGCATGCTT TCGCCAATCA CTTCAAAGAT TCTTATCAAG 
AAGTTGGGCA TCGCCGAGCA GGACGATCCC CTAATGATGA ATTTGAAGCG GACCATTTCC 
AGTGTCCTCC AGGCGCACGT TATATCCAAT GACAATCTGA CCGCTGCTGC ATTGTTGGAC 
CCACGCTTCC ACCGCCTGAC CACCATTGAC AACCTAGAGC GAACCGTTCG TATGCTGACC 
CATAAGTATA ACATTAACTT TGGTGGAGTG GGCGAAGGGG AATCCAACGA AGTGGCAGCT 
ACCTCCAGCG TGGTGGCCAT TAAGTCAGAA CCAAGAGTAG TGGACGGCAG TGCACCAAAG 
AAGTTGGGTC TGAAACTGCT GTTTGACAGT AACGAGATAC CAAATCCTCC GAAGCGAGAT 
GCGGACAGCA GCGTGGAGTC CGATCTTAAG CGATATCGCA ACGAGGTGGT CGTTCAGCTG 
GATGAGTCGC CCATCGAGTG GTGGCTCAAG ATGGGACACA TTTATGGAAC GCTCCGCGAT 
TTGGCCAGCC TGTACCATAG TGTGCCCGGC GTGGTGACGC TCAGCTTCAA GAAGGCGCTG 
AGAGACCAAA TATACGACTT CAACAAGCGA TTCATGCTCA CCGGTAGTCA CATCGACGCC 
ATCCTCTTTC TTCATCATCA CAACAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
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The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
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Dref cDNA ORF clone, Drosophila melanogaster(fruit fly)

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ORF Insert Sequence:

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ORF Insert Sequence:

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