D12 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	D12
Entrez Gene ID	34172
Full Name	CG13400 gene product from transcript CG13400-RA
Synonyms	CG13400,Dmel\CG13400,Yeats2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 29C4-29C4

mRNA and Protein(s)

mRNA	Protein	Name
NM_135384.3	NP_609228.2	D12

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following D12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the D12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa16481	NM_135384.3 Latest version!	Drosophila melanogaster D12 (D12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

D12 ( NM_135384.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609228.2 Drosophila melanogaster D12 (D12), mRNA.

Price & Availability↑

CloneID	OFa16481
Clone ID Related Accession (Same CDS sequence)	NM_135384.3
Accession Version	NM_135384.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	D12
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_135384.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACCAGC AACAAGCGAC GCCGCCAAAA AAACGCAGGC ACAGCGAGTA CCACGATCCA 
GATTATCAGC AAACGGAAGT TTCTAGCCAG GATGAGGAAG AAACCTGCGC AGAAGCCGAG 
TCGCCCGGTC AAAGAAAGTG GACCAAGACG GAACAGTCGG ATGGCATGCT GCCGTTGTCC 
GACAAATTTC AGCGCATCCG GGAGCTGCTT CGTCTGGAGT TCCAGCGCGA AATCTCTCAG 
AAGGTCGAGC AGCTGGCCGA GATCGACCGA CGACTGCTGC AAGGACGACA ACTGTTGGAC 
AGACTACGCT ATCAGGTGGT CAGCGAATAT TACCGAAAGC AGCAGGTCCC CCTCACCGGC 
GCGGATATTG CGAAAGTGCG CGGCGACAGC CTTTTTGGGG ACGACATAGC CGCTCCGCAG 
CTGCCACTGC ATCCAGCCAT TAAAAAAATC GTTGGCAAGC GCCCTGTTGT CATCCAAAAT 
CATTTGCCAG AGCGCACGGC GGCCACCTTG GCCAAAGAAA CCATCCGACT GAGGAATCCG 
GCTCATCGTC GGGCGGAACG AAGGCGGCAA CAGAAGATCA AGGAGCAGGG CATCGTAACC 
GATCACTCCA AGGACAAAAA AGATCAGCCA GAGCAGGAAC CTCCCATTTC CGTTAAGTTG 
GAGGACGAGC AGCCGTGCAC CAGCCGACAG GCCCACGAAA GGCAAGTGGA GCTGAATGCC 
TCGCGGCTGA ATAACAAGAA CAAATTCAAC TTCGTGGTGG GTAATACTTC CAAATACATT 
GGCGAAGACT CTCGAGAGAA TGGCACAGGT GGGAATGCTT TGACCTATAA GTGGTTGGTC 
TATGTGCAAG GCAAAGATCT ACCTGAACCA CTTGAAAAAT ACATTAAAAA GGTGCGCTTC 
CACCTCCATC CGTCATACCG CCCAAACGAC ATCGTGGACG TGCACAGGTC TCCGTTTCAG 
CTTAACCGAC ATGGTTGGGG CGAGTTTCCG ATGCGCATCC AGCTATTTTT CCAAGAGCAC 
TTGCAGCAGA AACCCGTGCA ACTAATGCAC ACGGTGGTGC TGGATAAAAC AATGTGTGGA 
CTGCACACGA TGGGCGCAGA GACCACTGTG GAAATATGGC TGAGAGCAAA ACAGGCGATA 
ACGAAACAGA AAAGCGGCAA ACCTCATCCT CCCCATGAAC AGGAGACTGC AGTTCCTGCT 
CCACCTCTGG CTGCTCCGAA TGTTTTAGAG CCTTCGGTTT TCGCCTTTCC TGGAGAGTCA 
TGCAAACCGC GCACCATTTC AATCACTCAA AATAAGGAGG AACTTGATGA CAATCTGTTT 
GCTGGTATCA ACAAAATTGA ACTCAGCGAC GACATTGAGA AAATTGAACC AACTGTGTTG 
GTTTCAGAGC CCCTGAAGTT AAACTACAGT CCAAGAAAGC AGCCACCCAC TCCGTCATCG 
CCTCCTAGGA CTCAACTTCG GTTAAATGCG GCTCAGGTCA CCGCTTCGAA GCCTTCTGTG 
GTCTATCTGC CAGTAAACGG CCGAAGTCCT AGGCAGGAAT CCTTGCCAGA AAGGCAGCGG 
CAGGAGTTTT CGCCAGTAAA GCACTATCCA CCGGCATCTC CGCAGAGAGC GTGGAAGGAA 
TCCTCGTCGG ATCGGCCACC AATAAAGCCT GTGCCCAATG GCCATCACCA GGGCAAGAAG 
AATGTTGTGT TTCAGAGAGC AGGAAAGCTG TACATTATCG ATCCCTTGCA GCGCAAGCTT 
AAGCAGGCTG CAAAGCAACA ATCCCTGCTG AAACCACAAT TGAGTCTTCT TAAGCCACCG 
TCAGAAACGC GATGGCATAT GCTGCAGTGC ATGCAGCACG ATCATGGGTA CGCCAACATG 
AGCGGCGAGA TGGAGGAAAA GCCAATGCTG TTACCTCCAA TTGTGGACAC TACCATACAA 
TCGCGACCCC GAAGGCTGGA GCACATCTTT AGAAGTGCAC AATTCCGAAA TATGCGCAGT 
GCTGTGGAGT TCCTGCTGAG CCGCCTCCCG CTTGCGTCGA TTGATCAGCA AAAGGAGTAT 
CCATTCACTT GTATAACCCT CGAAGAGTTC CACAATCACA CTGCTCTAAA ACAACGTTGC 
TTTGAGTACC TGCGCGCACG TATGCTCCGT CGCTGTCTGA TGCAGCACCA CGAGCTGCAT 
AAGTTGGACA TTAGTGGAAA GGAACACTAC TGGAGTTTAC GGGAGATAGT GGCGTTCGCC 
AGGGTTCATG GTTATACGCC AGCTCTCAAA GATGTTTTAC CTGTCGTGCA TGAAAAGAGA 
AAACAAAAAC TGAGCGATGA GGAGCAACTG GCTCGGCGTG TTCAGGCGCA ACTAAAGGGC 
GAGCCCCAAC CGCAACTGTC GGCCTATAGC AGTCTCAGCT CAGGCAATCG GCTGGAAGCT 
TGGATAACCA AGCAATCCGA TCGTCTGCTT GGTCATGATC AACAGATGCG GGATAAGGAG 
TTCATCGATG TGTTGGGTGT GGACAAACCT CATCCGCTTG CGTCCAGGTT AACAAACTCA 
GCAGATGTAT CTTTCCAAAT GGTGAATCAT CGCCAACTAC TTTATCTACC TCCGCCGAAG 
CACTTGGATT CGACCTTGCA GCTGGTCCAG GAAATGTGCA AGGACATTAA TATCACACTG 
GAACCGGAGG AATCTCCGCC TGGCGTCTCC CAGCCACTGG CTCTCTCGAT GCTCGGACAA 
GTGCTGCGCA CTTTTATTGA GAAATTGGTG CGCCGCTCCT TGGCGGCGAA ACTGCAACAG 
GAGACTTTGG AACAGTTGCC ACCGGCGGCG GCCAATGCTC CGTTGTGTCT CCAACCTCAG 
GACATAGGCC GTGTCATCAG TCTGTGTTCC GAATTGGATT TCCTCGGAAA CAGTCACTTG 
GGAGTTGCTC CGATAGAACC ACAAATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609228.2
CDS	191..3100
Translation	MDQQQATPPKKRRHSEYHDPDYQQTEVSSQDEEETCAEAESPGQRKWTKTEQSDGMLPLSDKFQRIRELLRLEFQREISQKVEQLAEIDRRLLQGRQLLDRLRYQVVSEYYRKQQVPLTGADIAKVRGDSLFGDDIAAPQLPLHPAIKKIVGKRPVVIQNHLPERTAATLAKETIRLRNPAHRRAERRRQQKIKEQGIVTDHSKDKKDQPEQEPPISVKLEDEQPCTSRQAHERQVELNASRLNNKNKFNFVVGNTSKYIGEDSRENGTGGNALTYKWLVYVQGKDLPEPLEKYIKKVRFHLHPSYRPNDIVDVHRSPFQLNRHGWGEFPMRIQLFFQEHLQQKPVQLMHTVVLDKTMCGLHTMGAETTVEIWLRAKQAITKQKSGKPHPPHEQETAVPAPPLAAPNVLEPSVFAFPGESCKPRTISITQNKEELDDNLFAGINKIELSDDIEKIEPTVLVSEPLKLNYSPRKQPPTPSSPPRTQLRLNAAQVTASKPSVVYLPVNGRSPRQESLPERQRQEFSPVKHYPPASPQRAWKESSSDRPPIKPVPNGHHQGKKNVVFQRAGKLYIIDPLQRKLKQAAKQQSLLKPQLSLLKPPSETRWHMLQCMQHDHGYANMSGEMEEKPMLLPPIVDTTIQSRPRRLEHIFRSAQFRNMRSAVEFLLSRLPLASIDQQKEYPFTCITLEEFHNHTALKQRCFEYLRARMLRRCLMQHHELHKLDISGKEHYWSLREIVAFARVHGYTPALKDVLPVVHEKRKQKLSDEEQLARRVQAQLKGEPQPQLSAYSSLSSGNRLEAWITKQSDRLLGHDQQMRDKEFIDVLGVDKPHPLASRLTNSADVSFQMVNHRQLLYLPPPKHLDSTLQLVQEMCKDINITLEPEESPPGVSQPLALSMLGQVLRTFIEKLVRRSLAAKLQQETLEQLPPAAANAPLCLQPQDIGRVISLCSELDFLGNSHLGVAPIEPQI

Target ORF information:

RefSeq Version	NM_135384.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster D12 (D12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135384.3

ORF Insert Sequence:

ATGGACCAGC AACAAGCGAC GCCGCCAAAA AAACGCAGGC ACAGCGAGTA CCACGATCCA 
GATTATCAGC AAACGGAAGT TTCTAGCCAG GATGAGGAAG AAACCTGCGC AGAAGCCGAG 
TCGCCCGGTC AAAGAAAGTG GACCAAGACG GAACAGTCGG ATGGCATGCT GCCGTTGTCC 
GACAAATTTC AGCGCATCCG GGAGCTGCTT CGTCTGGAGT TCCAGCGCGA AATCTCTCAG 
AAGGTCGAGC AGCTGGCCGA GATCGACCGA CGACTGCTGC AAGGACGACA ACTGTTGGAC 
AGACTACGCT ATCAGGTGGT CAGCGAATAT TACCGAAAGC AGCAGGTCCC CCTCACCGGC 
GCGGATATTG CGAAAGTGCG CGGCGACAGC CTTTTTGGGG ACGACATAGC CGCTCCGCAG 
CTGCCACTGC ATCCAGCCAT TAAAAAAATC GTTGGCAAGC GCCCTGTTGT CATCCAAAAT 
CATTTGCCAG AGCGCACGGC GGCCACCTTG GCCAAAGAAA CCATCCGACT GAGGAATCCG 
GCTCATCGTC GGGCGGAACG AAGGCGGCAA CAGAAGATCA AGGAGCAGGG CATCGTAACC 
GATCACTCCA AGGACAAAAA AGATCAGCCA GAGCAGGAAC CTCCCATTTC CGTTAAGTTG 
GAGGACGAGC AGCCGTGCAC CAGCCGACAG GCCCACGAAA GGCAAGTGGA GCTGAATGCC 
TCGCGGCTGA ATAACAAGAA CAAATTCAAC TTCGTGGTGG GTAATACTTC CAAATACATT 
GGCGAAGACT CTCGAGAGAA TGGCACAGGT GGGAATGCTT TGACCTATAA GTGGTTGGTC 
TATGTGCAAG GCAAAGATCT ACCTGAACCA CTTGAAAAAT ACATTAAAAA GGTGCGCTTC 
CACCTCCATC CGTCATACCG CCCAAACGAC ATCGTGGACG TGCACAGGTC TCCGTTTCAG 
CTTAACCGAC ATGGTTGGGG CGAGTTTCCG ATGCGCATCC AGCTATTTTT CCAAGAGCAC 
TTGCAGCAGA AACCCGTGCA ACTAATGCAC ACGGTGGTGC TGGATAAAAC AATGTGTGGA 
CTGCACACGA TGGGCGCAGA GACCACTGTG GAAATATGGC TGAGAGCAAA ACAGGCGATA 
ACGAAACAGA AAAGCGGCAA ACCTCATCCT CCCCATGAAC AGGAGACTGC AGTTCCTGCT 
CCACCTCTGG CTGCTCCGAA TGTTTTAGAG CCTTCGGTTT TCGCCTTTCC TGGAGAGTCA 
TGCAAACCGC GCACCATTTC AATCACTCAA AATAAGGAGG AACTTGATGA CAATCTGTTT 
GCTGGTATCA ACAAAATTGA ACTCAGCGAC GACATTGAGA AAATTGAACC AACTGTGTTG 
GTTTCAGAGC CCCTGAAGTT AAACTACAGT CCAAGAAAGC AGCCACCCAC TCCGTCATCG 
CCTCCTAGGA CTCAACTTCG GTTAAATGCG GCTCAGGTCA CCGCTTCGAA GCCTTCTGTG 
GTCTATCTGC CAGTAAACGG CCGAAGTCCT AGGCAGGAAT CCTTGCCAGA AAGGCAGCGG 
CAGGAGTTTT CGCCAGTAAA GCACTATCCA CCGGCATCTC CGCAGAGAGC GTGGAAGGAA 
TCCTCGTCGG ATCGGCCACC AATAAAGCCT GTGCCCAATG GCCATCACCA GGGCAAGAAG 
AATGTTGTGT TTCAGAGAGC AGGAAAGCTG TACATTATCG ATCCCTTGCA GCGCAAGCTT 
AAGCAGGCTG CAAAGCAACA ATCCCTGCTG AAACCACAAT TGAGTCTTCT TAAGCCACCG 
TCAGAAACGC GATGGCATAT GCTGCAGTGC ATGCAGCACG ATCATGGGTA CGCCAACATG 
AGCGGCGAGA TGGAGGAAAA GCCAATGCTG TTACCTCCAA TTGTGGACAC TACCATACAA 
TCGCGACCCC GAAGGCTGGA GCACATCTTT AGAAGTGCAC AATTCCGAAA TATGCGCAGT 
GCTGTGGAGT TCCTGCTGAG CCGCCTCCCG CTTGCGTCGA TTGATCAGCA AAAGGAGTAT 
CCATTCACTT GTATAACCCT CGAAGAGTTC CACAATCACA CTGCTCTAAA ACAACGTTGC 
TTTGAGTACC TGCGCGCACG TATGCTCCGT CGCTGTCTGA TGCAGCACCA CGAGCTGCAT 
AAGTTGGACA TTAGTGGAAA GGAACACTAC TGGAGTTTAC GGGAGATAGT GGCGTTCGCC 
AGGGTTCATG GTTATACGCC AGCTCTCAAA GATGTTTTAC CTGTCGTGCA TGAAAAGAGA 
AAACAAAAAC TGAGCGATGA GGAGCAACTG GCTCGGCGTG TTCAGGCGCA ACTAAAGGGC 
GAGCCCCAAC CGCAACTGTC GGCCTATAGC AGTCTCAGCT CAGGCAATCG GCTGGAAGCT 
TGGATAACCA AGCAATCCGA TCGTCTGCTT GGTCATGATC AACAGATGCG GGATAAGGAG 
TTCATCGATG TGTTGGGTGT GGACAAACCT CATCCGCTTG CGTCCAGGTT AACAAACTCA 
GCAGATGTAT CTTTCCAAAT GGTGAATCAT CGCCAACTAC TTTATCTACC TCCGCCGAAG 
CACTTGGATT CGACCTTGCA GCTGGTCCAG GAAATGTGCA AGGACATTAA TATCACACTG 
GAACCGGAGG AATCTCCGCC TGGCGTCTCC CAGCCACTGG CTCTCTCGAT GCTCGGACAA 
GTGCTGCGCA CTTTTATTGA GAAATTGGTG CGCCGCTCCT TGGCGGCGAA ACTGCAACAG 
GAGACTTTGG AACAGTTGCC ACCGGCGGCG GCCAATGCTC CGTTGTGTCT CCAACCTCAG 
GACATAGGCC GTGTCATCAG TCTGTGTTCC GAATTGGATT TCCTCGGAAA CAGTCACTTG 
GGAGTTGCTC CGATAGAACC ACAAATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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D12 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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