r2d2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	r2d2
Entrez Gene ID	34066
Full Name	CG7138 gene product from transcript CG7138-RA
Synonyms	CG7138,Dmel\CG7138,R2D2,R2d2,cg7138
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 28C4-28C4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001298791.1	NP_001285720.1	r2d2, isoform C
NM_135308.2	NP_609152.1	r2d2, isoform A
NM_001169432.2	NP_001162903.1	r2d2, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	r2d2	NP_609152.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009887	XP_001238071.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Partial reconstitution of the RNAi response in human cells has been demonstrated using Drosophila gene products, DCR-2, Loqs-PD, and R2D2.
Title: Partial reconstitution of the RNAi response in human cells using Drosophila gene products.

R2D2 and Loqs-PD have partially overlapping function.
Title: Transposon defense by endo-siRNAs, piRNAs and somatic pilRNAs in Drosophila: contributions of Loqs-PD and R2D2.

R2D2 controls endo-siRNA sorting by localizing Dcr-2, and presumably endo-siRNA duplexes, to cytoplasmic foci.
Title: Roles of R2D2, a cytoplasmic D2 body component, in the endogenous siRNA pathway in Drosophila.

Propose that the Dicer-2 helicase domain uses ATP to generate many siRNAs from a single molecule of dsRNA and that phosphate and R2D2 restrict substrate specificity.
Title: Phosphate and R2D2 restrict the substrate specificity of Dicer-2, an ATP-driven ribonuclease.

direct role for the R2D2/Dcr-2 heterodimer in sensing central mismatch positions that direct miRNA* strands to AGO2
Title: R2D2 organizes small regulatory RNA pathways in Drosophila.

The following r2d2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the r2d2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa14054	NM_001298791.1 Latest version!	Drosophila melanogaster r2d2, transcript variant C (r2d2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa14054	NM_135308.2 Latest version!	Drosophila melanogaster r2d2, transcript variant A (r2d2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa14054	NM_001169432.2 Latest version!	Drosophila melanogaster r2d2, transcript variant B (r2d2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

r2d2 ( NM_001298791.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285720.1 Drosophila melanogaster r2d2, transcript variant C (r2d2), mRNA.

Price & Availability↑

CloneID	OFa14054
Clone ID Related Accession (Same CDS sequence)	NM_001298791.1 , NM_135308.2 , NM_001169432.2
Accession Version	NM_001298791.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	r2d2, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATAACA AGTCAGCCGT ATCTGCTCTA CAGGAGTTTT GTGCCCGGAC ACAGATTAAT 
CTACCAACAT ACAGTTTTAT TCCCGGCGAA GACGGAGGGT ACGTCTGTAA AGTTGAACTA 
TTGGAGATAG AGGCCCTTGG AAATGGGCGT TCGAAGCGTG ATGCCAAACA CCTGGCTGCC 
AGCAATATCT TGCGTAAAAT CCAACTGCTG CCCGGCATAC ACGGCTTGAT GAAGGATTCG 
ACTGTGGGTG ATCTGGATGA GGAACTGACT AACCTCAACC GGGACATGGT GAAGGAGCTG 
CGTGACTACT GCGTCCGCCG CGAGATGCCA CTGCCCTGCA TTGAGGTAGT GCAGCAAAGC 
GGCACCCCGA GCGCCCCGGA ATTCGTGGCC TGTTGCTCCG TGGCCTCCAT AGTACGCTAC 
GGAAAGTCGG ACAAAAAGAA GGATGCCCGT CAGCGAGCGG CCATTGAAAT GCTGGCCTTA 
ATCTCCAGCA ATTCGGACAA TTTGCGTCCG GATCAAATGC AAGTAGCGAG CACAAGCAAA 
TTGAAAGTTG TTGATATGGA AGAATCTATG GAGGAATTGG AGGCATTGCG CAGAAAGAAA 
TTTACCACCT ACTGGGAGTT GAAGGAAGCC GGGAGCGTAG ACCATACAGG CATGCGGCTC 
TGCGACCGAC ACAACTACTT CAAGAACTTC TATCCTACCC TGAAAAAGGA GGCCATTGAG 
GCCATCAATT CAGATGAATA CGAGAGCTCC AAGGATAAGG CTATGGACGT AATGAGCTCT 
TTAAAGATAA CACCCAAAAT CAGTGAAGTG GAATCTTCAT CGTTGGTTCC CTTGCTTAGC 
GTCGAGCTTA ATTGTGCATT CGACGTGGTC CTTATGGCAA AGGAGACCGA TATCTACGAC 
CATATAATAG ACTATTTTCG CACCATGTTG ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285720.1
CDS	357..1292
Translation	MDNKSAVSALQEFCARTQINLPTYSFIPGEDGGYVCKVELLEIEALGNGRSKRDAKHLAASNILRKIQLLPGIHGLMKDSTVGDLDEELTNLNRDMVKELRDYCVRREMPLPCIEVVQQSGTPSAPEFVACCSVASIVRYGKSDKKKDARQRAAIEMLALISSNSDNLRPDQMQVASTSKLKVVDMEESMEELEALRRKKFTTYWELKEAGSVDHTGMRLCDRHNYFKNFYPTLKKEAIEAINSDEYESSKDKAMDVMSSLKITPKISEVESSSLVPLLSVELNCAFDVVLMAKETDIYDHIIDYFRTMLI

Target ORF information:

RefSeq Version	NM_001298791.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster r2d2, transcript variant C (r2d2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298791.1

ORF Insert Sequence:

ATGGATAACA AGTCAGCCGT ATCTGCTCTA CAGGAGTTTT GTGCCCGGAC ACAGATTAAT 
CTACCAACAT ACAGTTTTAT TCCCGGCGAA GACGGAGGGT ACGTCTGTAA AGTTGAACTA 
TTGGAGATAG AGGCCCTTGG AAATGGGCGT TCGAAGCGTG ATGCCAAACA CCTGGCTGCC 
AGCAATATCT TGCGTAAAAT CCAACTGCTG CCCGGCATAC ACGGCTTGAT GAAGGATTCG 
ACTGTGGGTG ATCTGGATGA GGAACTGACT AACCTCAACC GGGACATGGT GAAGGAGCTG 
CGTGACTACT GCGTCCGCCG CGAGATGCCA CTGCCCTGCA TTGAGGTAGT GCAGCAAAGC 
GGCACCCCGA GCGCCCCGGA ATTCGTGGCC TGTTGCTCCG TGGCCTCCAT AGTACGCTAC 
GGAAAGTCGG ACAAAAAGAA GGATGCCCGT CAGCGAGCGG CCATTGAAAT GCTGGCCTTA 
ATCTCCAGCA ATTCGGACAA TTTGCGTCCG GATCAAATGC AAGTAGCGAG CACAAGCAAA 
TTGAAAGTTG TTGATATGGA AGAATCTATG GAGGAATTGG AGGCATTGCG CAGAAAGAAA 
TTTACCACCT ACTGGGAGTT GAAGGAAGCC GGGAGCGTAG ACCATACAGG CATGCGGCTC 
TGCGACCGAC ACAACTACTT CAAGAACTTC TATCCTACCC TGAAAAAGGA GGCCATTGAG 
GCCATCAATT CAGATGAATA CGAGAGCTCC AAGGATAAGG CTATGGACGT AATGAGCTCT 
TTAAAGATAA CACCCAAAAT CAGTGAAGTG GAATCTTCAT CGTTGGTTCC CTTGCTTAGC 
GTCGAGCTTA ATTGTGCATT CGACGTGGTC CTTATGGCAA AGGAGACCGA TATCTACGAC 
CATATAATAG ACTATTTTCG CACCATGTTG ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

r2d2 ( NM_135308.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609152.1 Drosophila melanogaster r2d2, transcript variant A (r2d2), mRNA.

Price & Availability↑

CloneID	OFa14054
Clone ID Related Accession (Same CDS sequence)	NM_001298791.1 , NM_135308.2 , NM_001169432.2
Accession Version	NM_135308.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	r2d2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_135308.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATAACA AGTCAGCCGT ATCTGCTCTA CAGGAGTTTT GTGCCCGGAC ACAGATTAAT 
CTACCAACAT ACAGTTTTAT TCCCGGCGAA GACGGAGGGT ACGTCTGTAA AGTTGAACTA 
TTGGAGATAG AGGCCCTTGG AAATGGGCGT TCGAAGCGTG ATGCCAAACA CCTGGCTGCC 
AGCAATATCT TGCGTAAAAT CCAACTGCTG CCCGGCATAC ACGGCTTGAT GAAGGATTCG 
ACTGTGGGTG ATCTGGATGA GGAACTGACT AACCTCAACC GGGACATGGT GAAGGAGCTG 
CGTGACTACT GCGTCCGCCG CGAGATGCCA CTGCCCTGCA TTGAGGTAGT GCAGCAAAGC 
GGCACCCCGA GCGCCCCGGA ATTCGTGGCC TGTTGCTCCG TGGCCTCCAT AGTACGCTAC 
GGAAAGTCGG ACAAAAAGAA GGATGCCCGT CAGCGAGCGG CCATTGAAAT GCTGGCCTTA 
ATCTCCAGCA ATTCGGACAA TTTGCGTCCG GATCAAATGC AAGTAGCGAG CACAAGCAAA 
TTGAAAGTTG TTGATATGGA AGAATCTATG GAGGAATTGG AGGCATTGCG CAGAAAGAAA 
TTTACCACCT ACTGGGAGTT GAAGGAAGCC GGGAGCGTAG ACCATACAGG CATGCGGCTC 
TGCGACCGAC ACAACTACTT CAAGAACTTC TATCCTACCC TGAAAAAGGA GGCCATTGAG 
GCCATCAATT CAGATGAATA CGAGAGCTCC AAGGATAAGG CTATGGACGT AATGAGCTCT 
TTAAAGATAA CACCCAAAAT CAGTGAAGTG GAATCTTCAT CGTTGGTTCC CTTGCTTAGC 
GTCGAGCTTA ATTGTGCATT CGACGTGGTC CTTATGGCAA AGGAGACCGA TATCTACGAC 
CATATAATAG ACTATTTTCG CACCATGTTG ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609152.1
CDS	264..1199
Translation	MDNKSAVSALQEFCARTQINLPTYSFIPGEDGGYVCKVELLEIEALGNGRSKRDAKHLAASNILRKIQLLPGIHGLMKDSTVGDLDEELTNLNRDMVKELRDYCVRREMPLPCIEVVQQSGTPSAPEFVACCSVASIVRYGKSDKKKDARQRAAIEMLALISSNSDNLRPDQMQVASTSKLKVVDMEESMEELEALRRKKFTTYWELKEAGSVDHTGMRLCDRHNYFKNFYPTLKKEAIEAINSDEYESSKDKAMDVMSSLKITPKISEVESSSLVPLLSVELNCAFDVVLMAKETDIYDHIIDYFRTMLI

Target ORF information:

RefSeq Version	NM_135308.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster r2d2, transcript variant A (r2d2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135308.2

ORF Insert Sequence:

ATGGATAACA AGTCAGCCGT ATCTGCTCTA CAGGAGTTTT GTGCCCGGAC ACAGATTAAT 
CTACCAACAT ACAGTTTTAT TCCCGGCGAA GACGGAGGGT ACGTCTGTAA AGTTGAACTA 
TTGGAGATAG AGGCCCTTGG AAATGGGCGT TCGAAGCGTG ATGCCAAACA CCTGGCTGCC 
AGCAATATCT TGCGTAAAAT CCAACTGCTG CCCGGCATAC ACGGCTTGAT GAAGGATTCG 
ACTGTGGGTG ATCTGGATGA GGAACTGACT AACCTCAACC GGGACATGGT GAAGGAGCTG 
CGTGACTACT GCGTCCGCCG CGAGATGCCA CTGCCCTGCA TTGAGGTAGT GCAGCAAAGC 
GGCACCCCGA GCGCCCCGGA ATTCGTGGCC TGTTGCTCCG TGGCCTCCAT AGTACGCTAC 
GGAAAGTCGG ACAAAAAGAA GGATGCCCGT CAGCGAGCGG CCATTGAAAT GCTGGCCTTA 
ATCTCCAGCA ATTCGGACAA TTTGCGTCCG GATCAAATGC AAGTAGCGAG CACAAGCAAA 
TTGAAAGTTG TTGATATGGA AGAATCTATG GAGGAATTGG AGGCATTGCG CAGAAAGAAA 
TTTACCACCT ACTGGGAGTT GAAGGAAGCC GGGAGCGTAG ACCATACAGG CATGCGGCTC 
TGCGACCGAC ACAACTACTT CAAGAACTTC TATCCTACCC TGAAAAAGGA GGCCATTGAG 
GCCATCAATT CAGATGAATA CGAGAGCTCC AAGGATAAGG CTATGGACGT AATGAGCTCT 
TTAAAGATAA CACCCAAAAT CAGTGAAGTG GAATCTTCAT CGTTGGTTCC CTTGCTTAGC 
GTCGAGCTTA ATTGTGCATT CGACGTGGTC CTTATGGCAA AGGAGACCGA TATCTACGAC 
CATATAATAG ACTATTTTCG CACCATGTTG ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

r2d2 ( NM_001169432.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162903.1 Drosophila melanogaster r2d2, transcript variant B (r2d2), mRNA.

Price & Availability↑

CloneID	OFa14054
Clone ID Related Accession (Same CDS sequence)	NM_001298791.1 , NM_135308.2 , NM_001169432.2
Accession Version	NM_001169432.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	r2d2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001169432.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATAACA AGTCAGCCGT ATCTGCTCTA CAGGAGTTTT GTGCCCGGAC ACAGATTAAT 
CTACCAACAT ACAGTTTTAT TCCCGGCGAA GACGGAGGGT ACGTCTGTAA AGTTGAACTA 
TTGGAGATAG AGGCCCTTGG AAATGGGCGT TCGAAGCGTG ATGCCAAACA CCTGGCTGCC 
AGCAATATCT TGCGTAAAAT CCAACTGCTG CCCGGCATAC ACGGCTTGAT GAAGGATTCG 
ACTGTGGGTG ATCTGGATGA GGAACTGACT AACCTCAACC GGGACATGGT GAAGGAGCTG 
CGTGACTACT GCGTCCGCCG CGAGATGCCA CTGCCCTGCA TTGAGGTAGT GCAGCAAAGC 
GGCACCCCGA GCGCCCCGGA ATTCGTGGCC TGTTGCTCCG TGGCCTCCAT AGTACGCTAC 
GGAAAGTCGG ACAAAAAGAA GGATGCCCGT CAGCGAGCGG CCATTGAAAT GCTGGCCTTA 
ATCTCCAGCA ATTCGGACAA TTTGCGTCCG GATCAAATGC AAGTAGCGAG CACAAGCAAA 
TTGAAAGTTG TTGATATGGA AGAATCTATG GAGGAATTGG AGGCATTGCG CAGAAAGAAA 
TTTACCACCT ACTGGGAGTT GAAGGAAGCC GGGAGCGTAG ACCATACAGG CATGCGGCTC 
TGCGACCGAC ACAACTACTT CAAGAACTTC TATCCTACCC TGAAAAAGGA GGCCATTGAG 
GCCATCAATT CAGATGAATA CGAGAGCTCC AAGGATAAGG CTATGGACGT AATGAGCTCT 
TTAAAGATAA CACCCAAAAT CAGTGAAGTG GAATCTTCAT CGTTGGTTCC CTTGCTTAGC 
GTCGAGCTTA ATTGTGCATT CGACGTGGTC CTTATGGCAA AGGAGACCGA TATCTACGAC 
CATATAATAG ACTATTTTCG CACCATGTTG ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162903.1
CDS	122..1057
Translation	MDNKSAVSALQEFCARTQINLPTYSFIPGEDGGYVCKVELLEIEALGNGRSKRDAKHLAASNILRKIQLLPGIHGLMKDSTVGDLDEELTNLNRDMVKELRDYCVRREMPLPCIEVVQQSGTPSAPEFVACCSVASIVRYGKSDKKKDARQRAAIEMLALISSNSDNLRPDQMQVASTSKLKVVDMEESMEELEALRRKKFTTYWELKEAGSVDHTGMRLCDRHNYFKNFYPTLKKEAIEAINSDEYESSKDKAMDVMSSLKITPKISEVESSSLVPLLSVELNCAFDVVLMAKETDIYDHIIDYFRTMLI

Target ORF information:

RefSeq Version	NM_001169432.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster r2d2, transcript variant B (r2d2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169432.2

ORF Insert Sequence:

ATGGATAACA AGTCAGCCGT ATCTGCTCTA CAGGAGTTTT GTGCCCGGAC ACAGATTAAT 
CTACCAACAT ACAGTTTTAT TCCCGGCGAA GACGGAGGGT ACGTCTGTAA AGTTGAACTA 
TTGGAGATAG AGGCCCTTGG AAATGGGCGT TCGAAGCGTG ATGCCAAACA CCTGGCTGCC 
AGCAATATCT TGCGTAAAAT CCAACTGCTG CCCGGCATAC ACGGCTTGAT GAAGGATTCG 
ACTGTGGGTG ATCTGGATGA GGAACTGACT AACCTCAACC GGGACATGGT GAAGGAGCTG 
CGTGACTACT GCGTCCGCCG CGAGATGCCA CTGCCCTGCA TTGAGGTAGT GCAGCAAAGC 
GGCACCCCGA GCGCCCCGGA ATTCGTGGCC TGTTGCTCCG TGGCCTCCAT AGTACGCTAC 
GGAAAGTCGG ACAAAAAGAA GGATGCCCGT CAGCGAGCGG CCATTGAAAT GCTGGCCTTA 
ATCTCCAGCA ATTCGGACAA TTTGCGTCCG GATCAAATGC AAGTAGCGAG CACAAGCAAA 
TTGAAAGTTG TTGATATGGA AGAATCTATG GAGGAATTGG AGGCATTGCG CAGAAAGAAA 
TTTACCACCT ACTGGGAGTT GAAGGAAGCC GGGAGCGTAG ACCATACAGG CATGCGGCTC 
TGCGACCGAC ACAACTACTT CAAGAACTTC TATCCTACCC TGAAAAAGGA GGCCATTGAG 
GCCATCAATT CAGATGAATA CGAGAGCTCC AAGGATAAGG CTATGGACGT AATGAGCTCT 
TTAAAGATAA CACCCAAAAT CAGTGAAGTG GAATCTTCAT CGTTGGTTCC CTTGCTTAGC 
GTCGAGCTTA ATTGTGCATT CGACGTGGTC CTTATGGCAA AGGAGACCGA TATCTACGAC 
CATATAATAG ACTATTTTCG CACCATGTTG ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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r2d2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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