x16 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	x16
Entrez Gene ID	33967
Full Name	x16 splicing factor
Synonyms	2/30,9G8,CG10203,Dmel\CG10203,Dx16,Dxl6,RBP1-like,TN166,X16,X16/9G8,XL6,Xl6,anon-WO0118547.78,d9G8,dx16,dxl6,l(2)k00230,xl6
General protein information	Preferred Names x16 splicing factor Names CG10203 gene product from transcript CG10203-RB CG10203-PA CG10203-PB x16-PA x16-PB xl6
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 27C4-27C4

mRNA and Protein(s)

mRNA	Protein	Name
NM_164718.2	NP_723226.1	x16 splicing factor, isoform A
NM_001273239.2	NP_001260168.1	x16 splicing factor, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DX16 could be a novel serine-arginine-rich protein phosphorylated by DOA and it may participate in the formation of splicing complex through its interactions with other splicing related proteins
Title: DX16 is a novel SR protein phosphorylated by DOA.

These results indicate that Dxl6 has unique functions in the removal of some introns during development.
Title: Identification of RNA binding sequences of Drosophila SR protein DX16.

The following x16 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the x16 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13970	NM_001273239.2 Latest version!	Drosophila melanogaster x16 splicing factor (x16), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa16346	NM_164718.2 Latest version!	Drosophila melanogaster x16 splicing factor (x16), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

x16 ( NM_001273239.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260168.1 Drosophila melanogaster x16 splicing factor (x16), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa13970
Clone ID Related Accession (Same CDS sequence)	NM_001273239.2
Accession Version	NM_001273239.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	x16 splicing factor, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001273239.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCGCC ATCCGAGCGA TAGAAAGGTG TACGTGGGCG ATCTGGGCAA CAATGCCCGG 
AAGAACGACC TGGAGTATGT ATTTGGAGCG TACGGCAGTT TGCGCAGCGT CTGGATAGCC 
CGCAATCCGC CGGGCTTCGC CTTCGTGGAG TTTGAGAGTG CCCGCGATGC GGCGGATGCG 
GTGCGCGGAT TGGACGGACG GACGGTTTGC GGGCGCCGAG CCCGTGTGGA ATTGTCCACC 
GGAAAGTATG CTAGGTCCGG CGGTGGTGGT GGCGGAGGTG GTGGAGGCGG TGGTGGTGGA 
GGACTCGGAG GACGCGACCG AGGCGGCGGT GGTCGTGGGG ACGATAAGTG CTACGAGTGC 
GGCGGACGGG GGCATTTCGC TCGCCACTGT CGCGAAAGGA AGGCCAGGCA GCGACGCAGC 
AACTCATTCA GCAGATCTCG GAGCACATCG CGACGCAGGC GCACTCGCTC CAAGTCCGGA 
ACTCGATCCC GCAGTCGCTC CGCCGGCTCG GTGGGCCGTC GCTCCGGCCG CTCGAACGGA 
CGGGACGAGA ACGGATCGGC GTCGAGGTAC AGCGACCACG AGCGCAACGG CAGTGGAGCG 
GTAGATTCAC CGCCGCCGCC AAAACGGCGC TATGAGGATG AGGACGATGA CCGGGTTAGG 
GGATCGCCGC GGTCGCGTTC CCGATCTCGA TCGGCGTCGC CAGCGGTGCG CCGTGGATCG 
CCGCCCAGGC GTCGTGGTGA CTCGTCCGCC TCACGATCCG TTTCAAGGGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260168.1
CDS	269..1042
Translation	MSRHPSDRKVYVGDLGNNARKNDLEYVFGAYGSLRSVWIARNPPGFAFVEFESARDAADAVRGLDGRTVCGRRARVELSTGKYARSGGGGGGGGGGGGGGGLGGRDRGGGGRGDDKCYECGGRGHFARHCRERKARQRRSNSFSRSRSTSRRRRTRSKSGTRSRSRSAGSVGRRSGRSNGRDENGSASRYSDHERNGSGAVDSPPPPKRRYEDEDDDRVRGSPRSRSRSRSASPAVRRGSPPRRRGDSSASRSVSRD

Target ORF information:

RefSeq Version	NM_001273239.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster x16 splicing factor (x16), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273239.2

ORF Insert Sequence:

ATGTCGCGCC ATCCGAGCGA TAGAAAGGTG TACGTGGGCG ATCTGGGCAA CAATGCCCGG 
AAGAACGACC TGGAGTATGT ATTTGGAGCG TACGGCAGTT TGCGCAGCGT CTGGATAGCC 
CGCAATCCGC CGGGCTTCGC CTTCGTGGAG TTTGAGAGTG CCCGCGATGC GGCGGATGCG 
GTGCGCGGAT TGGACGGACG GACGGTTTGC GGGCGCCGAG CCCGTGTGGA ATTGTCCACC 
GGAAAGTATG CTAGGTCCGG CGGTGGTGGT GGCGGAGGTG GTGGAGGCGG TGGTGGTGGA 
GGACTCGGAG GACGCGACCG AGGCGGCGGT GGTCGTGGGG ACGATAAGTG CTACGAGTGC 
GGCGGACGGG GGCATTTCGC TCGCCACTGT CGCGAAAGGA AGGCCAGGCA GCGACGCAGC 
AACTCATTCA GCAGATCTCG GAGCACATCG CGACGCAGGC GCACTCGCTC CAAGTCCGGA 
ACTCGATCCC GCAGTCGCTC CGCCGGCTCG GTGGGCCGTC GCTCCGGCCG CTCGAACGGA 
CGGGACGAGA ACGGATCGGC GTCGAGGTAC AGCGACCACG AGCGCAACGG CAGTGGAGCG 
GTAGATTCAC CGCCGCCGCC AAAACGGCGC TATGAGGATG AGGACGATGA CCGGGTTAGG 
GGATCGCCGC GGTCGCGTTC CCGATCTCGA TCGGCGTCGC CAGCGGTGCG CCGTGGATCG 
CCGCCCAGGC GTCGTGGTGA CTCGTCCGCC TCACGATCCG TTTCAAGGGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

x16 ( NM_164718.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723226.1 Drosophila melanogaster x16 splicing factor (x16), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa16346
Clone ID Related Accession (Same CDS sequence)	NM_164718.2
Accession Version	NM_164718.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	x16 splicing factor, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164718.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGCGCC ATCCGAGCGA TAGAAAGGTG TACGTGGGCG ATCTGGGCAA CAATGCCCGG 
AAGAACGACC TGGAGTATGT ATTTGGAGCG TACGGCAGTT TGCGCAGCGT CTGGATAGCC 
CGCAATCCGC CGGGCTTCGC CTTCGTGGAG TTTGAGAGTG CCCGCGATGC GGCGGATGCG 
GTGCGCGGAT TGGACGGACG GACGGTTTGC GGGCGCCGAG CCCGTGTGGA ATTGTCCACC 
GGAAAGTATG CTAGGTCCGG CGGTGGTGGT GGCGGAGGTG GTGGAGGCGG TGGTGGTGGA 
GGACTCGGAG GACGCGACCG AGGCGGCGGT GGTCGTGGGG ACGATAAGTG CTACGAGTGC 
GGCGGACGGG GGCATTTCGC TCGCCACTGT CGCGAAAGGA AGGCCAGGCA GCGACGCAGA 
AGCAACTCAT TCAGCAGATC TCGGAGCACA TCGCGACGCA GGCGCACTCG CTCCAAGTCC 
GGAACTCGAT CCCGCAGTCG CTCCGCCGGC TCGGTGGGCC GTCGCTCCGG CCGCTCGAAC 
GGACGGGACG AGAACGGATC GGCGTCGAGG TACAGCGACC ACGAGCGCAA CGGCAGTGGA 
GCGGTAGATT CACCGCCGCC GCCAAAACGG CGCTATGAGG ATGAGGACGA TGACCGGGTT 
AGGGGATCGC CGCGGTCGCG TTCCCGATCT CGATCGGCGT CGCCAGCGGT GCGCCGTGGA 
TCGCCGCCCA GGCGTCGTGG TGACTCGTCC GCCTCACGAT CCGTTTCAAG GGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723226.1
CDS	269..1045
Translation	MSRHPSDRKVYVGDLGNNARKNDLEYVFGAYGSLRSVWIARNPPGFAFVEFESARDAADAVRGLDGRTVCGRRARVELSTGKYARSGGGGGGGGGGGGGGGLGGRDRGGGGRGDDKCYECGGRGHFARHCRERKARQRRRSNSFSRSRSTSRRRRTRSKSGTRSRSRSAGSVGRRSGRSNGRDENGSASRYSDHERNGSGAVDSPPPPKRRYEDEDDDRVRGSPRSRSRSRSASPAVRRGSPPRRRGDSSASRSVSRD

Target ORF information:

RefSeq Version	NM_164718.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster x16 splicing factor (x16), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164718.2

ORF Insert Sequence:

ATGTCGCGCC ATCCGAGCGA TAGAAAGGTG TACGTGGGCG ATCTGGGCAA CAATGCCCGG 
AAGAACGACC TGGAGTATGT ATTTGGAGCG TACGGCAGTT TGCGCAGCGT CTGGATAGCC 
CGCAATCCGC CGGGCTTCGC CTTCGTGGAG TTTGAGAGTG CCCGCGATGC GGCGGATGCG 
GTGCGCGGAT TGGACGGACG GACGGTTTGC GGGCGCCGAG CCCGTGTGGA ATTGTCCACC 
GGAAAGTATG CTAGGTCCGG CGGTGGTGGT GGCGGAGGTG GTGGAGGCGG TGGTGGTGGA 
GGACTCGGAG GACGCGACCG AGGCGGCGGT GGTCGTGGGG ACGATAAGTG CTACGAGTGC 
GGCGGACGGG GGCATTTCGC TCGCCACTGT CGCGAAAGGA AGGCCAGGCA GCGACGCAGA 
AGCAACTCAT TCAGCAGATC TCGGAGCACA TCGCGACGCA GGCGCACTCG CTCCAAGTCC 
GGAACTCGAT CCCGCAGTCG CTCCGCCGGC TCGGTGGGCC GTCGCTCCGG CCGCTCGAAC 
GGACGGGACG AGAACGGATC GGCGTCGAGG TACAGCGACC ACGAGCGCAA CGGCAGTGGA 
GCGGTAGATT CACCGCCGCC GCCAAAACGG CGCTATGAGG ATGAGGACGA TGACCGGGTT 
AGGGGATCGC CGCGGTCGCG TTCCCGATCT CGATCGGCGT CGCCAGCGGT GCGCCGTGGA 
TCGCCGCCCA GGCGTCGTGG TGACTCGTCC GCCTCACGAT CCGTTTCAAG GGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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x16 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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