H15 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	H15
Entrez Gene ID	33769
Full Name	CG6604 gene product from transcript CG6604-RA
Synonyms	CG6604,Dm-H15,Dmel\CG6604,Nmr,Nmr-1,Tbx20,dm-H15,nmr,nmr1,nmr[H15]
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 25E1-25E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_135082.3	NP_608926.2	H15, isoform A
NM_001298698.1	NP_001285627.1	H15, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	H15	NP_608926.2
Rattus norvegicus (Norway rat)	Tbx20	NP_001101602.1
Drosophila melanogaster (fruit fly)	mid	NP_001260096.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008569	XP_314675.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results suggest that midline and H15 may be considered as the selector genes for ventral leg fate.
Title: The Tbx20 homologs midline and H15 specify ventral fate in the Drosophila melanogaster leg.

Nmr1 and nmr2 appear to act together as members of the combinatorial code of transcription factors that govern neuronal subtype identity in the central nervous system.
Title: Neuromancer1 and Neuromancer2 regulate cell fate specification in the developing embryonic CNS of Drosophila melanogaster.

nmr is a gene required in adult fly hearts for the maintenance of both normal myofibrillar architecture and cardiac physiology.
Title: Transcription factor neuromancer/TBX20 is required for cardiac function in Drosophila with implications for human heart disease.

The function of the HI5 protein in Drosophila melanogaster neurogensis is described.
Title: Functions of the segment polarity genes midline and H15 in Drosophila melanogaster neurogenesis.

one of the major functions of mid and H15 during cardioblast development is the re-activation of tin expression at a stage when the induction of tin by Dpp in the dorsal mesoderm has ceased
Title: Tbx20-related genes, mid and H15, are required for tinman expression, proper patterning, and normal differentiation of cardioblasts in Drosophila.

The following H15 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the H15 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa16272	NM_135082.3 Latest version!	Drosophila melanogaster H15, transcript variant A (H15), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OFa21595	NM_001298698.1 Latest version!	Drosophila melanogaster H15, transcript variant B (H15), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

H15 ( NM_135082.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_608926.2 Drosophila melanogaster H15, transcript variant A (H15), mRNA.

Price & Availability↑

CloneID	OFa16272
Clone ID Related Accession (Same CDS sequence)	NM_135082.3
Accession Version	NM_135082.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1983bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	H15, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_135082.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTTTTGA GCAATCAGCC GGCGAATACG AAACCGCAGC AAACGCCTTC GCCGTCGCAG 
ACGCAGAACT TCAAGTCGAA GCTGCAGCAG CAGATTGTTT CGGCAGCGGC GGCGGCAGCG 
GCGAACATAG CCAACGGCAG CAGCCATCAT CATCATCATC AGAACCATCA TCATCACCAT 
CCGCTGAACA ACCATCATAA TCACAATCAC AATCAGCACA ACATTTCCTT CGCCACGGAT 
TTCTCAATTG CGGCGATTAT GGCGCGCGGC GGAAACGCCC CAAGCAGCCG CGAACCTTCG 
GAACGCAGTC TGAGTCCCGC ATCTGTGGAG CGCTATTCAG GCCAGGATGC GGACGATGAT 
GTTGACGTTG ATGTGGTCGA CTGCAGTGAC TCGGAAATGC CGTCGGCAAC TGCGGCAGCA 
GCAGCGGCGG CAACAACAGC AGCGGCTGCA GCGGCAGCAT TGCAGGCGCA GCAACAAGCG 
CGTCAGGCAT TGCGTGTTGC ACAGCAACAA CAGCAGCAGC AGCAACAACA AAGGCAGCAG 
ACACATCACC ACGCAACAAC TGGCAAGCAA CAACGCCAAC ATCACAATCA TCACAGCAGC 
AACACCAACA ACAGCAGCAA CTCAGGCAAC AGCAACACCA ACAGCAAATC AAGCAGCCAG 
CGAGGGCGTT CTGCTGCCGC AGTGGGAGCC GCTGCCACGC CCTCGCCACC GCCGCCCCCG 
CCCTCGCAAA GTCCGGAGGA ACTCGAACGC CTGTCGCCAG AGGAATCACC AGCCCAGCAG 
CCCACGCCCA AGATAGTGGG CTCGTGCAAC TGCGACGATC TCACGCCCGT CCAGTGCCAC 
CTGGAGACCA AGGAGCTGTG GGATAAGTTC CATGAATTGG GCACTGAAAT GATCATTACC 
AAATCGGGCA GACGCATGTT TCCCACCGTT CGAGTGAGTT TCTCGGGTCC TTTGCGGCAA 
ATTCAGCCAG CTGACCGATA TGCCGTACTT TTGGATGTGG TACCACTTGA TTCGCGGCGA 
TACCGTTACG CCTACCATCG CAGTTCGTGG CTGGTAGCCG GAAAGGCGGA TCCGCCGCCA 
CCCTCCCGCA TTTACGCCCA TCCGGATTGT CCTCTGAGTC CGGAGGCACT GCGAAAACAG 
GTCGTCTCCT TCGAAAAGGT GAAGTTGACA AACAACGAGA TGGACAAGAG CGGACAGGTC 
GTGCTGAACT CAATGCACCG CTACCAGCCC AGGATCCACT TGGTCCGGTT GAGCCATGGC 
CAGAGCATAC CCGGCAGTCC CAAGGAGCTG CAGGATATGG ACCATAAAAC CTTCGTTTTC 
CCGGAGACCG TATTCACGGC CGTGACGGCC TATCAGAATC AATTGATTAC GAAACTGAAA 
ATCGATTCGA ATCCGTTTGC GAAGGGATTT CGCGATTCGT CGCGTCTCAG TGACTTTGAT 
AGGGATCCCA TGGATGCGTT CTTCTTCGAC CAGCACATGC GTACTGCTCC CCTGCGATTC 
TTTCCCGATC CGCTGATGTC CCAATTGACT CCTCAGGAGG CAGATGCCGC ATCAATGGCG 
CTGTTGGAGA AAGCCCGCCA GCACTTGCAG ATGTTTGGAC GATCGCCGTA CACGGAGATG 
TTGCTGCCAC ATCTGTATCA GCGATCGGCG GCACCGCCGC CGCCCCCACC GGCCCCCCAC 
CTCAGCGCCT TTCAGCTGGG AATGTGGCAG CAGCAGTGGC CCCAACTCAC CGCCGGATTC 
CTGGCCAGCG CCAATCAGCA GGCGGCCTTG GCCTTGGCAG CAGCGGGCGC CAATCGAACG 
CCACCACCTT CGATGGCGGT GGCGCCACCA GCTCCTGCCA CGCCCACCTC CTCGTGCGGT 
TCCGCCTCTC CGGATCTGAG GGCCAGACCC CAACTGAATC ATTATCCTCA GCGGTTCAGT 
CCGTATCAGG TGCCCCAGCA TCAGGCATCG CCACCAGCCT CCAATCGAGC AGAAAGTCCA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_608926.2
CDS	601..2583
Translation	MLLSNQPANTKPQQTPSPSQTQNFKSKLQQQIVSAAAAAAANIANGSSHHHHHQNHHHHHPLNNHHNHNHNQHNISFATDFSIAAIMARGGNAPSSREPSERSLSPASVERYSGQDADDDVDVDVVDCSDSEMPSATAAAAAAATTAAAAAAALQAQQQARQALRVAQQQQQQQQQQRQQTHHHATTGKQQRQHHNHHSSNTNNSSNSGNSNTNSKSSSQRGRSAAAVGAAATPSPPPPPPSQSPEELERLSPEESPAQQPTPKIVGSCNCDDLTPVQCHLETKELWDKFHELGTEMIITKSGRRMFPTVRVSFSGPLRQIQPADRYAVLLDVVPLDSRRYRYAYHRSSWLVAGKADPPPPSRIYAHPDCPLSPEALRKQVVSFEKVKLTNNEMDKSGQVVLNSMHRYQPRIHLVRLSHGQSIPGSPKELQDMDHKTFVFPETVFTAVTAYQNQLITKLKIDSNPFAKGFRDSSRLSDFDRDPMDAFFFDQHMRTAPLRFFPDPLMSQLTPQEADAASMALLEKARQHLQMFGRSPYTEMLLPHLYQRSAAPPPPPPAPHLSAFQLGMWQQQWPQLTAGFLASANQQAALALAAAGANRTPPPSMAVAPPAPATPTSSCGSASPDLRARPQLNHYPQRFSPYQVPQHQASPPASNRAESP

Target ORF information:

RefSeq Version	NM_135082.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster H15, transcript variant A (H15), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135082.3

ORF Insert Sequence:

ATGCTTTTGA GCAATCAGCC GGCGAATACG AAACCGCAGC AAACGCCTTC GCCGTCGCAG 
ACGCAGAACT TCAAGTCGAA GCTGCAGCAG CAGATTGTTT CGGCAGCGGC GGCGGCAGCG 
GCGAACATAG CCAACGGCAG CAGCCATCAT CATCATCATC AGAACCATCA TCATCACCAT 
CCGCTGAACA ACCATCATAA TCACAATCAC AATCAGCACA ACATTTCCTT CGCCACGGAT 
TTCTCAATTG CGGCGATTAT GGCGCGCGGC GGAAACGCCC CAAGCAGCCG CGAACCTTCG 
GAACGCAGTC TGAGTCCCGC ATCTGTGGAG CGCTATTCAG GCCAGGATGC GGACGATGAT 
GTTGACGTTG ATGTGGTCGA CTGCAGTGAC TCGGAAATGC CGTCGGCAAC TGCGGCAGCA 
GCAGCGGCGG CAACAACAGC AGCGGCTGCA GCGGCAGCAT TGCAGGCGCA GCAACAAGCG 
CGTCAGGCAT TGCGTGTTGC ACAGCAACAA CAGCAGCAGC AGCAACAACA AAGGCAGCAG 
ACACATCACC ACGCAACAAC TGGCAAGCAA CAACGCCAAC ATCACAATCA TCACAGCAGC 
AACACCAACA ACAGCAGCAA CTCAGGCAAC AGCAACACCA ACAGCAAATC AAGCAGCCAG 
CGAGGGCGTT CTGCTGCCGC AGTGGGAGCC GCTGCCACGC CCTCGCCACC GCCGCCCCCG 
CCCTCGCAAA GTCCGGAGGA ACTCGAACGC CTGTCGCCAG AGGAATCACC AGCCCAGCAG 
CCCACGCCCA AGATAGTGGG CTCGTGCAAC TGCGACGATC TCACGCCCGT CCAGTGCCAC 
CTGGAGACCA AGGAGCTGTG GGATAAGTTC CATGAATTGG GCACTGAAAT GATCATTACC 
AAATCGGGCA GACGCATGTT TCCCACCGTT CGAGTGAGTT TCTCGGGTCC TTTGCGGCAA 
ATTCAGCCAG CTGACCGATA TGCCGTACTT TTGGATGTGG TACCACTTGA TTCGCGGCGA 
TACCGTTACG CCTACCATCG CAGTTCGTGG CTGGTAGCCG GAAAGGCGGA TCCGCCGCCA 
CCCTCCCGCA TTTACGCCCA TCCGGATTGT CCTCTGAGTC CGGAGGCACT GCGAAAACAG 
GTCGTCTCCT TCGAAAAGGT GAAGTTGACA AACAACGAGA TGGACAAGAG CGGACAGGTC 
GTGCTGAACT CAATGCACCG CTACCAGCCC AGGATCCACT TGGTCCGGTT GAGCCATGGC 
CAGAGCATAC CCGGCAGTCC CAAGGAGCTG CAGGATATGG ACCATAAAAC CTTCGTTTTC 
CCGGAGACCG TATTCACGGC CGTGACGGCC TATCAGAATC AATTGATTAC GAAACTGAAA 
ATCGATTCGA ATCCGTTTGC GAAGGGATTT CGCGATTCGT CGCGTCTCAG TGACTTTGAT 
AGGGATCCCA TGGATGCGTT CTTCTTCGAC CAGCACATGC GTACTGCTCC CCTGCGATTC 
TTTCCCGATC CGCTGATGTC CCAATTGACT CCTCAGGAGG CAGATGCCGC ATCAATGGCG 
CTGTTGGAGA AAGCCCGCCA GCACTTGCAG ATGTTTGGAC GATCGCCGTA CACGGAGATG 
TTGCTGCCAC ATCTGTATCA GCGATCGGCG GCACCGCCGC CGCCCCCACC GGCCCCCCAC 
CTCAGCGCCT TTCAGCTGGG AATGTGGCAG CAGCAGTGGC CCCAACTCAC CGCCGGATTC 
CTGGCCAGCG CCAATCAGCA GGCGGCCTTG GCCTTGGCAG CAGCGGGCGC CAATCGAACG 
CCACCACCTT CGATGGCGGT GGCGCCACCA GCTCCTGCCA CGCCCACCTC CTCGTGCGGT 
TCCGCCTCTC CGGATCTGAG GGCCAGACCC CAACTGAATC ATTATCCTCA GCGGTTCAGT 
CCGTATCAGG TGCCCCAGCA TCAGGCATCG CCACCAGCCT CCAATCGAGC AGAAAGTCCA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

H15 ( NM_001298698.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285627.1 Drosophila melanogaster H15, transcript variant B (H15), mRNA.

Price & Availability↑

CloneID	OFa21595
Clone ID Related Accession (Same CDS sequence)	NM_001298698.1
Accession Version	NM_001298698.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	H15, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTTTTGA GCAATCAGCC GGCGAATACG AAACCGCAGC AAACGCCTTC GCCGTCGCAG 
ACGCAGAACT TCAAGTCGAA GCTGCAGCAG CAGATTGTTT CGGCAGCGGC GGCGGCAGCG 
GCGAACATAG CCAACGGCAG CAGCCATCAT CATCATCATC AGAACCATCA TCATCACCAT 
CCGCTGAACA ACCATCATAA TCACAATCAC AATCAGCACA ACATTTCCTT CGCCACGGAT 
TTCTCAATTG CGGCGATTAT GGCGCGCGGC GGAAACGCCC CAAGCAGCCG CGAACCTTCG 
GAACGCAGTC TGAGTCCCGC ATCTGTGGAG CGCTATTCAG GCCAGGATGC GGACGATGAT 
GTTGACGTTG ATGTGGTCGA CTGCAGTGAC TCGGAAATGC CGTCGGCAAC TGCGGCAGCA 
GCAGCGGCGG CAACAACAGC AGCGGCTGCA GCGGCAGCAT TGCAGGCGCA GCAACAAGCG 
CGTCAGGCAT TGCGTGTTGC ACAGCAACAA CAGCAGCAGC AGCAACAACA AAGGCAGCAG 
ACACATCACC ACGCAACAAC TGGCAAGCAA CAACGCCAAC ATCACAATCA TCACAGCAGC 
AACACCAACA ACAGCAGCAA CTCAGGCAAC AGCAACACCA ACAGCAAATC AAGCAGCCAG 
CGAGGGCGTT CTGCTGCCGC AGTGGGAGCC GCTGCCACGC CCTCGCCACC GCCGCCCCCG 
CCCTCGCAAA GTCCGGAGGA ACTCGAACGC CTGTCGCCAG AGGAATCACC AGCCCAGCCC 
ACGCCCAAGA TAGTGGGCTC GTGCAACTGC GACGATCTCA CGCCCGTCCA GTGCCACCTG 
GAGACCAAGG AGCTGTGGGA TAAGTTCCAT GAATTGGGCA CTGAAATGAT CATTACCAAA 
TCGGGCAGAC GCATGTTTCC CACCGTTCGA GTGAGTTTCT CGGGTCCTTT GCGGCAAATT 
CAGCCAGCTG ACCGATATGC CGTACTTTTG GATGTGGTAC CACTTGATTC GCGGCGATAC 
CGTTACGCCT ACCATCGCAG TTCGTGGCTG GTAGCCGGAA AGGCGGATCC GCCGCCACCC 
TCCCGCATTT ACGCCCATCC GGATTGTCCT CTGAGTCCGG AGGCACTGCG AAAACAGGTC 
GTCTCCTTCG AAAAGGTGAA GTTGACAAAC AACGAGATGG ACAAGAGCGG ACAGGTCGTG 
CTGAACTCAA TGCACCGCTA CCAGCCCAGG ATCCACTTGG TCCGGTTGAG CCATGGCCAG 
AGCATACCCG GCAGTCCCAA GGAGCTGCAG GATATGGACC ATAAAACCTT CGTTTTCCCG 
GAGACCGTAT TCACGGCCGT GACGGCCTAT CAGAATCAAT TGATTACGAA ACTGAAAATC 
GATTCGAATC CGTTTGCGAA GGGATTTCGC GATTCGTCGC GTCTCAGTGA CTTTGATAGG 
GATCCCATGG ATGCGTTCTT CTTCGACCAG CACATGCGTA CTGCTCCCCT GCGATTCTTT 
CCCGATCCGC TGATGTCCCA ATTGACTCCT CAGGAGGCAG ATGCCGCATC AATGGCGCTG 
TTGGAGAAAG CCCGCCAGCA CTTGCAGATG TTTGGACGAT CGCCGTACAC GGAGATGTTG 
CTGCCACATC TGTATCAGCG ATCGGCGGCA CCGCCGCCGC CCCCACCGGC CCCCCACCTC 
AGCGCCTTTC AGCTGGGAAT GTGGCAGCAG CAGTGGCCCC AACTCACCGC CGGATTCCTG 
GCCAGCGCCA ATCAGCAGGC GGCCTTGGCC TTGGCAGCAG CGGGCGCCAA TCGAACGCCA 
CCACCTTCGA TGGCGGTGGC GCCACCAGCT CCTGCCACGC CCACCTCCTC GTGCGGTTCC 
GCCTCTCCGG ATCTGAGGGC CAGACCCCAA CTGAATCATT ATCCTCAGCG GTTCAGTCCG 
TATCAGGTGC CCCAGCATCA GGCATCGCCA CCAGCCTCCA ATCGAGCAGA AAGTCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285627.1
CDS	248..2227
Translation	MLLSNQPANTKPQQTPSPSQTQNFKSKLQQQIVSAAAAAAANIANGSSHHHHHQNHHHHHPLNNHHNHNHNQHNISFATDFSIAAIMARGGNAPSSREPSERSLSPASVERYSGQDADDDVDVDVVDCSDSEMPSATAAAAAAATTAAAAAAALQAQQQARQALRVAQQQQQQQQQQRQQTHHHATTGKQQRQHHNHHSSNTNNSSNSGNSNTNSKSSSQRGRSAAAVGAAATPSPPPPPPSQSPEELERLSPEESPAQPTPKIVGSCNCDDLTPVQCHLETKELWDKFHELGTEMIITKSGRRMFPTVRVSFSGPLRQIQPADRYAVLLDVVPLDSRRYRYAYHRSSWLVAGKADPPPPSRIYAHPDCPLSPEALRKQVVSFEKVKLTNNEMDKSGQVVLNSMHRYQPRIHLVRLSHGQSIPGSPKELQDMDHKTFVFPETVFTAVTAYQNQLITKLKIDSNPFAKGFRDSSRLSDFDRDPMDAFFFDQHMRTAPLRFFPDPLMSQLTPQEADAASMALLEKARQHLQMFGRSPYTEMLLPHLYQRSAAPPPPPPAPHLSAFQLGMWQQQWPQLTAGFLASANQQAALALAAAGANRTPPPSMAVAPPAPATPTSSCGSASPDLRARPQLNHYPQRFSPYQVPQHQASPPASNRAESP

Target ORF information:

RefSeq Version	NM_001298698.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster H15, transcript variant B (H15), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298698.1

ORF Insert Sequence:

ATGCTTTTGA GCAATCAGCC GGCGAATACG AAACCGCAGC AAACGCCTTC GCCGTCGCAG 
ACGCAGAACT TCAAGTCGAA GCTGCAGCAG CAGATTGTTT CGGCAGCGGC GGCGGCAGCG 
GCGAACATAG CCAACGGCAG CAGCCATCAT CATCATCATC AGAACCATCA TCATCACCAT 
CCGCTGAACA ACCATCATAA TCACAATCAC AATCAGCACA ACATTTCCTT CGCCACGGAT 
TTCTCAATTG CGGCGATTAT GGCGCGCGGC GGAAACGCCC CAAGCAGCCG CGAACCTTCG 
GAACGCAGTC TGAGTCCCGC ATCTGTGGAG CGCTATTCAG GCCAGGATGC GGACGATGAT 
GTTGACGTTG ATGTGGTCGA CTGCAGTGAC TCGGAAATGC CGTCGGCAAC TGCGGCAGCA 
GCAGCGGCGG CAACAACAGC AGCGGCTGCA GCGGCAGCAT TGCAGGCGCA GCAACAAGCG 
CGTCAGGCAT TGCGTGTTGC ACAGCAACAA CAGCAGCAGC AGCAACAACA AAGGCAGCAG 
ACACATCACC ACGCAACAAC TGGCAAGCAA CAACGCCAAC ATCACAATCA TCACAGCAGC 
AACACCAACA ACAGCAGCAA CTCAGGCAAC AGCAACACCA ACAGCAAATC AAGCAGCCAG 
CGAGGGCGTT CTGCTGCCGC AGTGGGAGCC GCTGCCACGC CCTCGCCACC GCCGCCCCCG 
CCCTCGCAAA GTCCGGAGGA ACTCGAACGC CTGTCGCCAG AGGAATCACC AGCCCAGCCC 
ACGCCCAAGA TAGTGGGCTC GTGCAACTGC GACGATCTCA CGCCCGTCCA GTGCCACCTG 
GAGACCAAGG AGCTGTGGGA TAAGTTCCAT GAATTGGGCA CTGAAATGAT CATTACCAAA 
TCGGGCAGAC GCATGTTTCC CACCGTTCGA GTGAGTTTCT CGGGTCCTTT GCGGCAAATT 
CAGCCAGCTG ACCGATATGC CGTACTTTTG GATGTGGTAC CACTTGATTC GCGGCGATAC 
CGTTACGCCT ACCATCGCAG TTCGTGGCTG GTAGCCGGAA AGGCGGATCC GCCGCCACCC 
TCCCGCATTT ACGCCCATCC GGATTGTCCT CTGAGTCCGG AGGCACTGCG AAAACAGGTC 
GTCTCCTTCG AAAAGGTGAA GTTGACAAAC AACGAGATGG ACAAGAGCGG ACAGGTCGTG 
CTGAACTCAA TGCACCGCTA CCAGCCCAGG ATCCACTTGG TCCGGTTGAG CCATGGCCAG 
AGCATACCCG GCAGTCCCAA GGAGCTGCAG GATATGGACC ATAAAACCTT CGTTTTCCCG 
GAGACCGTAT TCACGGCCGT GACGGCCTAT CAGAATCAAT TGATTACGAA ACTGAAAATC 
GATTCGAATC CGTTTGCGAA GGGATTTCGC GATTCGTCGC GTCTCAGTGA CTTTGATAGG 
GATCCCATGG ATGCGTTCTT CTTCGACCAG CACATGCGTA CTGCTCCCCT GCGATTCTTT 
CCCGATCCGC TGATGTCCCA ATTGACTCCT CAGGAGGCAG ATGCCGCATC AATGGCGCTG 
TTGGAGAAAG CCCGCCAGCA CTTGCAGATG TTTGGACGAT CGCCGTACAC GGAGATGTTG 
CTGCCACATC TGTATCAGCG ATCGGCGGCA CCGCCGCCGC CCCCACCGGC CCCCCACCTC 
AGCGCCTTTC AGCTGGGAAT GTGGCAGCAG CAGTGGCCCC AACTCACCGC CGGATTCCTG 
GCCAGCGCCA ATCAGCAGGC GGCCTTGGCC TTGGCAGCAG CGGGCGCCAA TCGAACGCCA 
CCACCTTCGA TGGCGGTGGC GCCACCAGCT CCTGCCACGC CCACCTCCTC GTGCGGTTCC 
GCCTCTCCGG ATCTGAGGGC CAGACCCCAA CTGAATCATT ATCCTCAGCG GTTCAGTCCG 
TATCAGGTGC CCCAGCATCA GGCATCGCCA CCAGCCTCCA ATCGAGCAGA AAGTCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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H15 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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