vri cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	vri
Entrez Gene ID	33759
Full Name	vrille
Synonyms	CG14029,DM16,Dmel\CG14029,VRI,Vri,argo,jf23,l(2)25Db,l(2)jf23,mat(2)ea-G,mat(2)earlyRS32,mel_vri
General protein information	Preferred Names vrille Names CG14029 gene product from transcript CG14029-RE CG14029-PA CG14029-PC CG14029-PD CG14029-PE CG14029-PF lethal(2)25Db maternal effect early arrest G vri-PA vri-PC vri-PD vri-PE vri-PF
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 25D4-25D5\|2-17 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_164639.2	NP_723075.1	vrille, isoform C
NM_001298694.1	NP_001285623.1	vrille, isoform E
NM_057843.4	NP_477191.1	vrille, isoform A
NM_001144320.2	NP_001137792.1	vrille, isoform D
NM_001298695.1	NP_001285624.1	vrille, isoform F

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we demonstrate that Vri, the ortholog of the vertebrate NFIL3/E4B4 transcription factor, is a major genetic regulator of cardiac aging. Vri overexpression leads to major heart dysfunctions
Title: dJun and Vri/dNFIL3 are major regulators of cardiac aging in Drosophila.

E23 forms its own feedback loop in the ecdysone response to control circadian oscillation through ecdysone-mediated vrille expression.
Title: Membrane-bound transporter controls the circadian transcription of clock genes in Drosophila.

Vrille mutants interfered with tracheal development, but did not produce defects in gut morphology.
Title: vrille is required to ensure tracheal integrity in Drosophila embryo.

Results show that three direct targets of ECR/USP--hairy, vrille, and Hr4--are required for cellular differentiation in response to the hormone.
Title: Genomic mapping of binding regions for the Ecdysone receptor protein complex.

Clk, vri, and Pdp-1 have roles in controlling Drosophila circadian oscillations, as shown in a simulation
Title: Simulation of Drosophila circadian oscillations, mutations, and light responses by a model with VRI, PDP-1, and CLK.

The following vri gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the vri cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa16263	NM_164639.2 Latest version!	Drosophila melanogaster vrille (vri), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa16263	NM_001298694.1 Latest version!	Drosophila melanogaster vrille (vri), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa13796	NM_001144320.2 Latest version!	Drosophila melanogaster vrille (vri), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa13796	NM_001298695.1 Latest version!	Drosophila melanogaster vrille (vri), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa13796	NM_057843.4 Latest version!	Drosophila melanogaster vrille (vri), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

vri ( NM_164639.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723075.1 Drosophila melanogaster vrille (vri), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa16263
Clone ID Related Accession (Same CDS sequence)	NM_164639.2 , NM_001298694.1
Accession Version	NM_164639.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vrille, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 26, 2009 this sequence version replaced NM_164639.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCGAGG TCCTCAGCGG CACGCCCACC AACAGCCACA ACAAAGCCAA CATCGCCATG 
ATGAACAACG TCCGGCTATC CAATATATCG CCGACTCTCT CGATGAACGG CAGCTCCAAC 
GAGGCTTCTA ACTTGCATCC GTTGTCCATG TACGGAGGAT CCATAAGTCC GCAGTCAAAT 
GACAGCGGCA TGTCCGACAG TCTGGGCAAA TATGTCCCGG GAAGCGGCTA TGGAGATGGA 
ATGATGGCCC AGTCGCCCTC ACAGGGTGGC AATGGACCGC AGTCGGCGTT GACCGCCGCC 
CAGAAAGAGC TCTTCTCGCA GCGAAAGCAA CGCGAATTCA CCCCGGACAA CAAGAAGGAC 
GAGAGCTACT GGGATCGCCG GAGAAGGAAC AACGAGGCGG CCAAGCGGAG TCGAGAGAAG 
AGGCGCTACA ATGACATGGT TCTGGAGCAG CGCGTCATCG AGCTGACCAA AGAGAACCAT 
GTGCTGAAGG CCCAGCTGGA CGCCATACGC GACAAGTTCA ACATCTCCGG CGAGAATCTG 
GTGAGCGTGG AGAAGATCCT CGCCTCGCTG CCCACCAGCG AACAGGTGCT GAGTAACACA 
AAGCGCGCCA AGATGAGCGG CAGTGGTGGC TCCTCCTCGG GCTCCTCGCC CTCGGGCAGT 
GGATCGGGCG AGGGATCGCC TCAAGGTGGC CACAATGGCT ATCCGGTGGG TCCACCACTA 
TCTCCACTGA TCTACGGACC CAATGGCAAT GCCCGGCCAG AGGCCACGGT TAAGAGTGTC 
CACCACATAC ATCATGCGGG TGTTGCGCCG CCACCAACGC ACCTACAACA GTTGGTTGTG 
CCCCAGTCCC AGACACAGCA TCTCTACCAG CCACAGCCGC AGCAGCATCA GCCGCATCAG 
CAGCAGCAGA TTTCCCAGCC ACCGCAGCAA CAACAGCAGC AGCAGGAGCC AAGTCCCAGT 
GCCGGCTCCA GTTCGCCAGT CATCTCCGAT CCGCACAACC GCCCGCCAAG CACCACCATT 
GCCAATCTCC AGGTGCAACT GCAGCAGGCA CTAAACCGCA ATGTCAGGCC CGAGGATCTG 
GACAGCTTGC GCAAGGTGGT GGCCGCCGGC GCTCTATACA ATGCGGCAGC CGTGGTGGGA 
GCACCACCAC CGCCACCATC TGCTGGTCTC TACGTGCCCG CTCCCAGCGC CTATAAGGAT 
CACCTGGAAG CGGCTGCAGC CTGGAGCCAC AATGTCGAGG CTGCTGTGAG CAGCAGTGCT 
GTGGATGCGG TCAGTAGCTC ATCGGTGTCC GGCAGTGCGG CCAGTGTGCT GAATCTATCG 
CGTCGGGCCT GCTCACCCAG CTACGAGCAC ATGCTCTCCT CCACCACCTC CTCCACTCTT 
TCCTCGGCCT CCTCATCGGG AGCTGTTTCC GGCGATGATG AGCAGGAGCA CGAACCAGCG 
CACATGGCTC CACTGCAGCT GCAGCGAAGC AGTCCGCAGC AGGGCAGCGA TGCCAACAAC 
TGTCTGCCCC TCAAGCTGCG CCACAAGTCC CATCTCGGCG ACAAGGATGC GGCGGCCACG 
GCGCTACTCT CACTGCAGCA CATCAAGCAG GAGCCCAACT GCAGTCGGGC ATCGCCGCCG 
GCTTGGAACG ATGGCGGCGA CAATTCCAGC GACGAAAGGG ACTCCGGCAT CTCCATCGCC 
AGTGCGGAGT GGACGGCGCA GCTGCAGAGG AAGCTACTGG CGCCCAAGGA GGCCAATGTG 
GTAACCAGTG CCGAGCGCGA TCAAATGCTC AAGTCGCAGC TGGAGCGCCT AGAGTCCGAG 
GTGGCCAGCA TCAAGATGAT CTTGGCGGAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723075.1
CDS	321..2153
Translation	MPEVLSGTPTNSHNKANIAMMNNVRLSNISPTLSMNGSSNEASNLHPLSMYGGSISPQSNDSGMSDSLGKYVPGSGYGDGMMAQSPSQGGNGPQSALTAAQKELFSQRKQREFTPDNKKDESYWDRRRRNNEAAKRSREKRRYNDMVLEQRVIELTKENHVLKAQLDAIRDKFNISGENLVSVEKILASLPTSEQVLSNTKRAKMSGSGGSSSGSSPSGSGSGEGSPQGGHNGYPVGPPLSPLIYGPNGNARPEATVKSVHHIHHAGVAPPPTHLQQLVVPQSQTQHLYQPQPQQHQPHQQQQISQPPQQQQQQQEPSPSAGSSSPVISDPHNRPPSTTIANLQVQLQQALNRNVRPEDLDSLRKVVAAGALYNAAAVVGAPPPPPSAGLYVPAPSAYKDHLEAAAAWSHNVEAAVSSSAVDAVSSSSVSGSAASVLNLSRRACSPSYEHMLSSTTSSTLSSASSSGAVSGDDEQEHEPAHMAPLQLQRSSPQQGSDANNCLPLKLRHKSHLGDKDAAATALLSLQHIKQEPNCSRASPPAWNDGGDNSSDERDSGISIASAEWTAQLQRKLLAPKEANVVTSAERDQMLKSQLERLESEVASIKMILAE

Target ORF information:

RefSeq Version	NM_164639.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vrille (vri), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164639.2

ORF Insert Sequence:

ATGCCCGAGG TCCTCAGCGG CACGCCCACC AACAGCCACA ACAAAGCCAA CATCGCCATG 
ATGAACAACG TCCGGCTATC CAATATATCG CCGACTCTCT CGATGAACGG CAGCTCCAAC 
GAGGCTTCTA ACTTGCATCC GTTGTCCATG TACGGAGGAT CCATAAGTCC GCAGTCAAAT 
GACAGCGGCA TGTCCGACAG TCTGGGCAAA TATGTCCCGG GAAGCGGCTA TGGAGATGGA 
ATGATGGCCC AGTCGCCCTC ACAGGGTGGC AATGGACCGC AGTCGGCGTT GACCGCCGCC 
CAGAAAGAGC TCTTCTCGCA GCGAAAGCAA CGCGAATTCA CCCCGGACAA CAAGAAGGAC 
GAGAGCTACT GGGATCGCCG GAGAAGGAAC AACGAGGCGG CCAAGCGGAG TCGAGAGAAG 
AGGCGCTACA ATGACATGGT TCTGGAGCAG CGCGTCATCG AGCTGACCAA AGAGAACCAT 
GTGCTGAAGG CCCAGCTGGA CGCCATACGC GACAAGTTCA ACATCTCCGG CGAGAATCTG 
GTGAGCGTGG AGAAGATCCT CGCCTCGCTG CCCACCAGCG AACAGGTGCT GAGTAACACA 
AAGCGCGCCA AGATGAGCGG CAGTGGTGGC TCCTCCTCGG GCTCCTCGCC CTCGGGCAGT 
GGATCGGGCG AGGGATCGCC TCAAGGTGGC CACAATGGCT ATCCGGTGGG TCCACCACTA 
TCTCCACTGA TCTACGGACC CAATGGCAAT GCCCGGCCAG AGGCCACGGT TAAGAGTGTC 
CACCACATAC ATCATGCGGG TGTTGCGCCG CCACCAACGC ACCTACAACA GTTGGTTGTG 
CCCCAGTCCC AGACACAGCA TCTCTACCAG CCACAGCCGC AGCAGCATCA GCCGCATCAG 
CAGCAGCAGA TTTCCCAGCC ACCGCAGCAA CAACAGCAGC AGCAGGAGCC AAGTCCCAGT 
GCCGGCTCCA GTTCGCCAGT CATCTCCGAT CCGCACAACC GCCCGCCAAG CACCACCATT 
GCCAATCTCC AGGTGCAACT GCAGCAGGCA CTAAACCGCA ATGTCAGGCC CGAGGATCTG 
GACAGCTTGC GCAAGGTGGT GGCCGCCGGC GCTCTATACA ATGCGGCAGC CGTGGTGGGA 
GCACCACCAC CGCCACCATC TGCTGGTCTC TACGTGCCCG CTCCCAGCGC CTATAAGGAT 
CACCTGGAAG CGGCTGCAGC CTGGAGCCAC AATGTCGAGG CTGCTGTGAG CAGCAGTGCT 
GTGGATGCGG TCAGTAGCTC ATCGGTGTCC GGCAGTGCGG CCAGTGTGCT GAATCTATCG 
CGTCGGGCCT GCTCACCCAG CTACGAGCAC ATGCTCTCCT CCACCACCTC CTCCACTCTT 
TCCTCGGCCT CCTCATCGGG AGCTGTTTCC GGCGATGATG AGCAGGAGCA CGAACCAGCG 
CACATGGCTC CACTGCAGCT GCAGCGAAGC AGTCCGCAGC AGGGCAGCGA TGCCAACAAC 
TGTCTGCCCC TCAAGCTGCG CCACAAGTCC CATCTCGGCG ACAAGGATGC GGCGGCCACG 
GCGCTACTCT CACTGCAGCA CATCAAGCAG GAGCCCAACT GCAGTCGGGC ATCGCCGCCG 
GCTTGGAACG ATGGCGGCGA CAATTCCAGC GACGAAAGGG ACTCCGGCAT CTCCATCGCC 
AGTGCGGAGT GGACGGCGCA GCTGCAGAGG AAGCTACTGG CGCCCAAGGA GGCCAATGTG 
GTAACCAGTG CCGAGCGCGA TCAAATGCTC AAGTCGCAGC TGGAGCGCCT AGAGTCCGAG 
GTGGCCAGCA TCAAGATGAT CTTGGCGGAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vri ( NM_001298694.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285623.1 Drosophila melanogaster vrille (vri), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa16263
Clone ID Related Accession (Same CDS sequence)	NM_164639.2 , NM_001298694.1
Accession Version	NM_001298694.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vrille, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCCGAGG TCCTCAGCGG CACGCCCACC AACAGCCACA ACAAAGCCAA CATCGCCATG 
ATGAACAACG TCCGGCTATC CAATATATCG CCGACTCTCT CGATGAACGG CAGCTCCAAC 
GAGGCTTCTA ACTTGCATCC GTTGTCCATG TACGGAGGAT CCATAAGTCC GCAGTCAAAT 
GACAGCGGCA TGTCCGACAG TCTGGGCAAA TATGTCCCGG GAAGCGGCTA TGGAGATGGA 
ATGATGGCCC AGTCGCCCTC ACAGGGTGGC AATGGACCGC AGTCGGCGTT GACCGCCGCC 
CAGAAAGAGC TCTTCTCGCA GCGAAAGCAA CGCGAATTCA CCCCGGACAA CAAGAAGGAC 
GAGAGCTACT GGGATCGCCG GAGAAGGAAC AACGAGGCGG CCAAGCGGAG TCGAGAGAAG 
AGGCGCTACA ATGACATGGT TCTGGAGCAG CGCGTCATCG AGCTGACCAA AGAGAACCAT 
GTGCTGAAGG CCCAGCTGGA CGCCATACGC GACAAGTTCA ACATCTCCGG CGAGAATCTG 
GTGAGCGTGG AGAAGATCCT CGCCTCGCTG CCCACCAGCG AACAGGTGCT GAGTAACACA 
AAGCGCGCCA AGATGAGCGG CAGTGGTGGC TCCTCCTCGG GCTCCTCGCC CTCGGGCAGT 
GGATCGGGCG AGGGATCGCC TCAAGGTGGC CACAATGGCT ATCCGGTGGG TCCACCACTA 
TCTCCACTGA TCTACGGACC CAATGGCAAT GCCCGGCCAG AGGCCACGGT TAAGAGTGTC 
CACCACATAC ATCATGCGGG TGTTGCGCCG CCACCAACGC ACCTACAACA GTTGGTTGTG 
CCCCAGTCCC AGACACAGCA TCTCTACCAG CCACAGCCGC AGCAGCATCA GCCGCATCAG 
CAGCAGCAGA TTTCCCAGCC ACCGCAGCAA CAACAGCAGC AGCAGGAGCC AAGTCCCAGT 
GCCGGCTCCA GTTCGCCAGT CATCTCCGAT CCGCACAACC GCCCGCCAAG CACCACCATT 
GCCAATCTCC AGGTGCAACT GCAGCAGGCA CTAAACCGCA ATGTCAGGCC CGAGGATCTG 
GACAGCTTGC GCAAGGTGGT GGCCGCCGGC GCTCTATACA ATGCGGCAGC CGTGGTGGGA 
GCACCACCAC CGCCACCATC TGCTGGTCTC TACGTGCCCG CTCCCAGCGC CTATAAGGAT 
CACCTGGAAG CGGCTGCAGC CTGGAGCCAC AATGTCGAGG CTGCTGTGAG CAGCAGTGCT 
GTGGATGCGG TCAGTAGCTC ATCGGTGTCC GGCAGTGCGG CCAGTGTGCT GAATCTATCG 
CGTCGGGCCT GCTCACCCAG CTACGAGCAC ATGCTCTCCT CCACCACCTC CTCCACTCTT 
TCCTCGGCCT CCTCATCGGG AGCTGTTTCC GGCGATGATG AGCAGGAGCA CGAACCAGCG 
CACATGGCTC CACTGCAGCT GCAGCGAAGC AGTCCGCAGC AGGGCAGCGA TGCCAACAAC 
TGTCTGCCCC TCAAGCTGCG CCACAAGTCC CATCTCGGCG ACAAGGATGC GGCGGCCACG 
GCGCTACTCT CACTGCAGCA CATCAAGCAG GAGCCCAACT GCAGTCGGGC ATCGCCGCCG 
GCTTGGAACG ATGGCGGCGA CAATTCCAGC GACGAAAGGG ACTCCGGCAT CTCCATCGCC 
AGTGCGGAGT GGACGGCGCA GCTGCAGAGG AAGCTACTGG CGCCCAAGGA GGCCAATGTG 
GTAACCAGTG CCGAGCGCGA TCAAATGCTC AAGTCGCAGC TGGAGCGCCT AGAGTCCGAG 
GTGGCCAGCA TCAAGATGAT CTTGGCGGAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285623.1
CDS	782..2614
Translation	MPEVLSGTPTNSHNKANIAMMNNVRLSNISPTLSMNGSSNEASNLHPLSMYGGSISPQSNDSGMSDSLGKYVPGSGYGDGMMAQSPSQGGNGPQSALTAAQKELFSQRKQREFTPDNKKDESYWDRRRRNNEAAKRSREKRRYNDMVLEQRVIELTKENHVLKAQLDAIRDKFNISGENLVSVEKILASLPTSEQVLSNTKRAKMSGSGGSSSGSSPSGSGSGEGSPQGGHNGYPVGPPLSPLIYGPNGNARPEATVKSVHHIHHAGVAPPPTHLQQLVVPQSQTQHLYQPQPQQHQPHQQQQISQPPQQQQQQQEPSPSAGSSSPVISDPHNRPPSTTIANLQVQLQQALNRNVRPEDLDSLRKVVAAGALYNAAAVVGAPPPPPSAGLYVPAPSAYKDHLEAAAAWSHNVEAAVSSSAVDAVSSSSVSGSAASVLNLSRRACSPSYEHMLSSTTSSTLSSASSSGAVSGDDEQEHEPAHMAPLQLQRSSPQQGSDANNCLPLKLRHKSHLGDKDAAATALLSLQHIKQEPNCSRASPPAWNDGGDNSSDERDSGISIASAEWTAQLQRKLLAPKEANVVTSAERDQMLKSQLERLESEVASIKMILAE

Target ORF information:

RefSeq Version	NM_001298694.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vrille (vri), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298694.1

ORF Insert Sequence:

ATGCCCGAGG TCCTCAGCGG CACGCCCACC AACAGCCACA ACAAAGCCAA CATCGCCATG 
ATGAACAACG TCCGGCTATC CAATATATCG CCGACTCTCT CGATGAACGG CAGCTCCAAC 
GAGGCTTCTA ACTTGCATCC GTTGTCCATG TACGGAGGAT CCATAAGTCC GCAGTCAAAT 
GACAGCGGCA TGTCCGACAG TCTGGGCAAA TATGTCCCGG GAAGCGGCTA TGGAGATGGA 
ATGATGGCCC AGTCGCCCTC ACAGGGTGGC AATGGACCGC AGTCGGCGTT GACCGCCGCC 
CAGAAAGAGC TCTTCTCGCA GCGAAAGCAA CGCGAATTCA CCCCGGACAA CAAGAAGGAC 
GAGAGCTACT GGGATCGCCG GAGAAGGAAC AACGAGGCGG CCAAGCGGAG TCGAGAGAAG 
AGGCGCTACA ATGACATGGT TCTGGAGCAG CGCGTCATCG AGCTGACCAA AGAGAACCAT 
GTGCTGAAGG CCCAGCTGGA CGCCATACGC GACAAGTTCA ACATCTCCGG CGAGAATCTG 
GTGAGCGTGG AGAAGATCCT CGCCTCGCTG CCCACCAGCG AACAGGTGCT GAGTAACACA 
AAGCGCGCCA AGATGAGCGG CAGTGGTGGC TCCTCCTCGG GCTCCTCGCC CTCGGGCAGT 
GGATCGGGCG AGGGATCGCC TCAAGGTGGC CACAATGGCT ATCCGGTGGG TCCACCACTA 
TCTCCACTGA TCTACGGACC CAATGGCAAT GCCCGGCCAG AGGCCACGGT TAAGAGTGTC 
CACCACATAC ATCATGCGGG TGTTGCGCCG CCACCAACGC ACCTACAACA GTTGGTTGTG 
CCCCAGTCCC AGACACAGCA TCTCTACCAG CCACAGCCGC AGCAGCATCA GCCGCATCAG 
CAGCAGCAGA TTTCCCAGCC ACCGCAGCAA CAACAGCAGC AGCAGGAGCC AAGTCCCAGT 
GCCGGCTCCA GTTCGCCAGT CATCTCCGAT CCGCACAACC GCCCGCCAAG CACCACCATT 
GCCAATCTCC AGGTGCAACT GCAGCAGGCA CTAAACCGCA ATGTCAGGCC CGAGGATCTG 
GACAGCTTGC GCAAGGTGGT GGCCGCCGGC GCTCTATACA ATGCGGCAGC CGTGGTGGGA 
GCACCACCAC CGCCACCATC TGCTGGTCTC TACGTGCCCG CTCCCAGCGC CTATAAGGAT 
CACCTGGAAG CGGCTGCAGC CTGGAGCCAC AATGTCGAGG CTGCTGTGAG CAGCAGTGCT 
GTGGATGCGG TCAGTAGCTC ATCGGTGTCC GGCAGTGCGG CCAGTGTGCT GAATCTATCG 
CGTCGGGCCT GCTCACCCAG CTACGAGCAC ATGCTCTCCT CCACCACCTC CTCCACTCTT 
TCCTCGGCCT CCTCATCGGG AGCTGTTTCC GGCGATGATG AGCAGGAGCA CGAACCAGCG 
CACATGGCTC CACTGCAGCT GCAGCGAAGC AGTCCGCAGC AGGGCAGCGA TGCCAACAAC 
TGTCTGCCCC TCAAGCTGCG CCACAAGTCC CATCTCGGCG ACAAGGATGC GGCGGCCACG 
GCGCTACTCT CACTGCAGCA CATCAAGCAG GAGCCCAACT GCAGTCGGGC ATCGCCGCCG 
GCTTGGAACG ATGGCGGCGA CAATTCCAGC GACGAAAGGG ACTCCGGCAT CTCCATCGCC 
AGTGCGGAGT GGACGGCGCA GCTGCAGAGG AAGCTACTGG CGCCCAAGGA GGCCAATGTG 
GTAACCAGTG CCGAGCGCGA TCAAATGCTC AAGTCGCAGC TGGAGCGCCT AGAGTCCGAG 
GTGGCCAGCA TCAAGATGAT CTTGGCGGAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vri ( NM_057843.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477191.1 Drosophila melanogaster vrille (vri), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa13796
Clone ID Related Accession (Same CDS sequence)	NM_057843.4 , NM_001144320.2 , NM_001298695.1
Accession Version	NM_057843.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vrille, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_057843.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGATTG TCTGCACGCT GGAACAGAAA GTGAACTTTT TCAAAGCGGC TGCAACGACC 
AAAAATCTGT TGATACTCAA AAACAATACG GATACGAATT ACATCAATAA CTACAAGCAG 
GATAATCCCA GCAACAATAA ATTTCCCCGC ATCCAAGCTC AAAGCAACAA CAGCCATCTG 
CAACACCAGC AGCAACTGCA GCAGAAACTA GCGCAGCTGC ATCACTACAG CCAGCAGAAG 
CTGAGCGGCA GCGACTTTCC CTACGGGCCA CGCCCCCCCA CCGGAGGCAA AGAGGAGAAG 
CTGCTCCTGC TAGCGCCGCC GGGCAAACTC TATCCGGAGG CGTCTGTGTC CACAGCAATG 
CCCGAGGTCC TCAGCGGCAC GCCCACCAAC AGCCACAACA AAGCCAACAT CGCCATGATG 
AACAACGTCC GGCTATCCAA TATATCGCCG ACTCTCTCGA TGAACGGCAG CTCCAACGAG 
GCTTCTAACT TGCATCCGTT GTCCATGTAC GGAGGATCCA TAAGTCCGCA GTCAAATGAC 
AGCGGCATGT CCGACAGTCT GGGCAAATAT GTCCCGGGAA GCGGCTATGG AGATGGAATG 
ATGGCCCAGT CGCCCTCACA GGGTGGCAAT GGACCGCAGT CGGCGTTGAC CGCCGCCCAG 
AAAGAGCTCT TCTCGCAGCG AAAGCAACGC GAATTCACCC CGGACAACAA GAAGGACGAG 
AGCTACTGGG ATCGCCGGAG AAGGAACAAC GAGGCGGCCA AGCGGAGTCG AGAGAAGAGG 
CGCTACAATG ACATGGTTCT GGAGCAGCGC GTCATCGAGC TGACCAAAGA GAACCATGTG 
CTGAAGGCCC AGCTGGACGC CATACGCGAC AAGTTCAACA TCTCCGGCGA GAATCTGGTG 
AGCGTGGAGA AGATCCTCGC CTCGCTGCCC ACCAGCGAAC AGGTGCTGAG TAACACAAAG 
CGCGCCAAGA TGAGCGGCAG TGGTGGCTCC TCCTCGGGCT CCTCGCCCTC GGGCAGTGGA 
TCGGGCGAGG GATCGCCTCA AGGTGGCCAC AATGGCTATC CGGTGGGTCC ACCACTATCT 
CCACTGATCT ACGGACCCAA TGGCAATGCC CGGCCAGAGG CCACGGTTAA GAGTGTCCAC 
CACATACATC ATGCGGGTGT TGCGCCGCCA CCAACGCACC TACAACAGTT GGTTGTGCCC 
CAGTCCCAGA CACAGCATCT CTACCAGCCA CAGCCGCAGC AGCATCAGCC GCATCAGCAG 
CAGCAGATTT CCCAGCCACC GCAGCAACAA CAGCAGCAGC AGGAGCCAAG TCCCAGTGCC 
GGCTCCAGTT CGCCAGTCAT CTCCGATCCG CACAACCGCC CGCCAAGCAC CACCATTGCC 
AATCTCCAGG TGCAACTGCA GCAGGCACTA AACCGCAATG TCAGGCCCGA GGATCTGGAC 
AGCTTGCGCA AGGTGGTGGC CGCCGGCGCT CTATACAATG CGGCAGCCGT GGTGGGAGCA 
CCACCACCGC CACCATCTGC TGGTCTCTAC GTGCCCGCTC CCAGCGCCTA TAAGGATCAC 
CTGGAAGCGG CTGCAGCCTG GAGCCACAAT GTCGAGGCTG CTGTGAGCAG CAGTGCTGTG 
GATGCGGTCA GTAGCTCATC GGTGTCCGGC AGTGCGGCCA GTGTGCTGAA TCTATCGCGT 
CGGGCCTGCT CACCCAGCTA CGAGCACATG CTCTCCTCCA CCACCTCCTC CACTCTTTCC 
TCGGCCTCCT CATCGGGAGC TGTTTCCGGC GATGATGAGC AGGAGCACGA ACCAGCGCAC 
ATGGCTCCAC TGCAGCTGCA GCGAAGCAGT CCGCAGCAGG GCAGCGATGC CAACAACTGT 
CTGCCCCTCA AGCTGCGCCA CAAGTCCCAT CTCGGCGACA AGGATGCGGC GGCCACGGCG 
CTACTCTCAC TGCAGCACAT CAAGCAGGAG CCCAACTGCA GTCGGGCATC GCCGCCGGCT 
TGGAACGATG GCGGCGACAA TTCCAGCGAC GAAAGGGACT CCGGCATCTC CATCGCCAGT 
GCGGAGTGGA CGGCGCAGCT GCAGAGGAAG CTACTGGCGC CCAAGGAGGC CAATGTGGTA 
ACCAGTGCCG AGCGCGATCA AATGCTCAAG TCGCAGCTGG AGCGCCTAGA GTCCGAGGTG 
GCCAGCATCA AGATGATCTT GGCGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477191.1
CDS	376..2565
Translation	MSIVCTLEQKVNFFKAAATTKNLLILKNNTDTNYINNYKQDNPSNNKFPRIQAQSNNSHLQHQQQLQQKLAQLHHYSQQKLSGSDFPYGPRPPTGGKEEKLLLLAPPGKLYPEASVSTAMPEVLSGTPTNSHNKANIAMMNNVRLSNISPTLSMNGSSNEASNLHPLSMYGGSISPQSNDSGMSDSLGKYVPGSGYGDGMMAQSPSQGGNGPQSALTAAQKELFSQRKQREFTPDNKKDESYWDRRRRNNEAAKRSREKRRYNDMVLEQRVIELTKENHVLKAQLDAIRDKFNISGENLVSVEKILASLPTSEQVLSNTKRAKMSGSGGSSSGSSPSGSGSGEGSPQGGHNGYPVGPPLSPLIYGPNGNARPEATVKSVHHIHHAGVAPPPTHLQQLVVPQSQTQHLYQPQPQQHQPHQQQQISQPPQQQQQQQEPSPSAGSSSPVISDPHNRPPSTTIANLQVQLQQALNRNVRPEDLDSLRKVVAAGALYNAAAVVGAPPPPPSAGLYVPAPSAYKDHLEAAAAWSHNVEAAVSSSAVDAVSSSSVSGSAASVLNLSRRACSPSYEHMLSSTTSSTLSSASSSGAVSGDDEQEHEPAHMAPLQLQRSSPQQGSDANNCLPLKLRHKSHLGDKDAAATALLSLQHIKQEPNCSRASPPAWNDGGDNSSDERDSGISIASAEWTAQLQRKLLAPKEANVVTSAERDQMLKSQLERLESEVASIKMILAE

Target ORF information:

RefSeq Version	NM_057843.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vrille (vri), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057843.4

ORF Insert Sequence:

ATGTCGATTG TCTGCACGCT GGAACAGAAA GTGAACTTTT TCAAAGCGGC TGCAACGACC 
AAAAATCTGT TGATACTCAA AAACAATACG GATACGAATT ACATCAATAA CTACAAGCAG 
GATAATCCCA GCAACAATAA ATTTCCCCGC ATCCAAGCTC AAAGCAACAA CAGCCATCTG 
CAACACCAGC AGCAACTGCA GCAGAAACTA GCGCAGCTGC ATCACTACAG CCAGCAGAAG 
CTGAGCGGCA GCGACTTTCC CTACGGGCCA CGCCCCCCCA CCGGAGGCAA AGAGGAGAAG 
CTGCTCCTGC TAGCGCCGCC GGGCAAACTC TATCCGGAGG CGTCTGTGTC CACAGCAATG 
CCCGAGGTCC TCAGCGGCAC GCCCACCAAC AGCCACAACA AAGCCAACAT CGCCATGATG 
AACAACGTCC GGCTATCCAA TATATCGCCG ACTCTCTCGA TGAACGGCAG CTCCAACGAG 
GCTTCTAACT TGCATCCGTT GTCCATGTAC GGAGGATCCA TAAGTCCGCA GTCAAATGAC 
AGCGGCATGT CCGACAGTCT GGGCAAATAT GTCCCGGGAA GCGGCTATGG AGATGGAATG 
ATGGCCCAGT CGCCCTCACA GGGTGGCAAT GGACCGCAGT CGGCGTTGAC CGCCGCCCAG 
AAAGAGCTCT TCTCGCAGCG AAAGCAACGC GAATTCACCC CGGACAACAA GAAGGACGAG 
AGCTACTGGG ATCGCCGGAG AAGGAACAAC GAGGCGGCCA AGCGGAGTCG AGAGAAGAGG 
CGCTACAATG ACATGGTTCT GGAGCAGCGC GTCATCGAGC TGACCAAAGA GAACCATGTG 
CTGAAGGCCC AGCTGGACGC CATACGCGAC AAGTTCAACA TCTCCGGCGA GAATCTGGTG 
AGCGTGGAGA AGATCCTCGC CTCGCTGCCC ACCAGCGAAC AGGTGCTGAG TAACACAAAG 
CGCGCCAAGA TGAGCGGCAG TGGTGGCTCC TCCTCGGGCT CCTCGCCCTC GGGCAGTGGA 
TCGGGCGAGG GATCGCCTCA AGGTGGCCAC AATGGCTATC CGGTGGGTCC ACCACTATCT 
CCACTGATCT ACGGACCCAA TGGCAATGCC CGGCCAGAGG CCACGGTTAA GAGTGTCCAC 
CACATACATC ATGCGGGTGT TGCGCCGCCA CCAACGCACC TACAACAGTT GGTTGTGCCC 
CAGTCCCAGA CACAGCATCT CTACCAGCCA CAGCCGCAGC AGCATCAGCC GCATCAGCAG 
CAGCAGATTT CCCAGCCACC GCAGCAACAA CAGCAGCAGC AGGAGCCAAG TCCCAGTGCC 
GGCTCCAGTT CGCCAGTCAT CTCCGATCCG CACAACCGCC CGCCAAGCAC CACCATTGCC 
AATCTCCAGG TGCAACTGCA GCAGGCACTA AACCGCAATG TCAGGCCCGA GGATCTGGAC 
AGCTTGCGCA AGGTGGTGGC CGCCGGCGCT CTATACAATG CGGCAGCCGT GGTGGGAGCA 
CCACCACCGC CACCATCTGC TGGTCTCTAC GTGCCCGCTC CCAGCGCCTA TAAGGATCAC 
CTGGAAGCGG CTGCAGCCTG GAGCCACAAT GTCGAGGCTG CTGTGAGCAG CAGTGCTGTG 
GATGCGGTCA GTAGCTCATC GGTGTCCGGC AGTGCGGCCA GTGTGCTGAA TCTATCGCGT 
CGGGCCTGCT CACCCAGCTA CGAGCACATG CTCTCCTCCA CCACCTCCTC CACTCTTTCC 
TCGGCCTCCT CATCGGGAGC TGTTTCCGGC GATGATGAGC AGGAGCACGA ACCAGCGCAC 
ATGGCTCCAC TGCAGCTGCA GCGAAGCAGT CCGCAGCAGG GCAGCGATGC CAACAACTGT 
CTGCCCCTCA AGCTGCGCCA CAAGTCCCAT CTCGGCGACA AGGATGCGGC GGCCACGGCG 
CTACTCTCAC TGCAGCACAT CAAGCAGGAG CCCAACTGCA GTCGGGCATC GCCGCCGGCT 
TGGAACGATG GCGGCGACAA TTCCAGCGAC GAAAGGGACT CCGGCATCTC CATCGCCAGT 
GCGGAGTGGA CGGCGCAGCT GCAGAGGAAG CTACTGGCGC CCAAGGAGGC CAATGTGGTA 
ACCAGTGCCG AGCGCGATCA AATGCTCAAG TCGCAGCTGG AGCGCCTAGA GTCCGAGGTG 
GCCAGCATCA AGATGATCTT GGCGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vri ( NM_001144320.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137792.1 Drosophila melanogaster vrille (vri), transcript variant D, mRNA.

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CloneID	OFa13796
Clone ID Related Accession (Same CDS sequence)	NM_057843.4 , NM_001144320.2 , NM_001298695.1
Accession Version	NM_001144320.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vrille, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_001144320.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGATTG TCTGCACGCT GGAACAGAAA GTGAACTTTT TCAAAGCGGC TGCAACGACC 
AAAAATCTGT TGATACTCAA AAACAATACG GATACGAATT ACATCAATAA CTACAAGCAG 
GATAATCCCA GCAACAATAA ATTTCCCCGC ATCCAAGCTC AAAGCAACAA CAGCCATCTG 
CAACACCAGC AGCAACTGCA GCAGAAACTA GCGCAGCTGC ATCACTACAG CCAGCAGAAG 
CTGAGCGGCA GCGACTTTCC CTACGGGCCA CGCCCCCCCA CCGGAGGCAA AGAGGAGAAG 
CTGCTCCTGC TAGCGCCGCC GGGCAAACTC TATCCGGAGG CGTCTGTGTC CACAGCAATG 
CCCGAGGTCC TCAGCGGCAC GCCCACCAAC AGCCACAACA AAGCCAACAT CGCCATGATG 
AACAACGTCC GGCTATCCAA TATATCGCCG ACTCTCTCGA TGAACGGCAG CTCCAACGAG 
GCTTCTAACT TGCATCCGTT GTCCATGTAC GGAGGATCCA TAAGTCCGCA GTCAAATGAC 
AGCGGCATGT CCGACAGTCT GGGCAAATAT GTCCCGGGAA GCGGCTATGG AGATGGAATG 
ATGGCCCAGT CGCCCTCACA GGGTGGCAAT GGACCGCAGT CGGCGTTGAC CGCCGCCCAG 
AAAGAGCTCT TCTCGCAGCG AAAGCAACGC GAATTCACCC CGGACAACAA GAAGGACGAG 
AGCTACTGGG ATCGCCGGAG AAGGAACAAC GAGGCGGCCA AGCGGAGTCG AGAGAAGAGG 
CGCTACAATG ACATGGTTCT GGAGCAGCGC GTCATCGAGC TGACCAAAGA GAACCATGTG 
CTGAAGGCCC AGCTGGACGC CATACGCGAC AAGTTCAACA TCTCCGGCGA GAATCTGGTG 
AGCGTGGAGA AGATCCTCGC CTCGCTGCCC ACCAGCGAAC AGGTGCTGAG TAACACAAAG 
CGCGCCAAGA TGAGCGGCAG TGGTGGCTCC TCCTCGGGCT CCTCGCCCTC GGGCAGTGGA 
TCGGGCGAGG GATCGCCTCA AGGTGGCCAC AATGGCTATC CGGTGGGTCC ACCACTATCT 
CCACTGATCT ACGGACCCAA TGGCAATGCC CGGCCAGAGG CCACGGTTAA GAGTGTCCAC 
CACATACATC ATGCGGGTGT TGCGCCGCCA CCAACGCACC TACAACAGTT GGTTGTGCCC 
CAGTCCCAGA CACAGCATCT CTACCAGCCA CAGCCGCAGC AGCATCAGCC GCATCAGCAG 
CAGCAGATTT CCCAGCCACC GCAGCAACAA CAGCAGCAGC AGGAGCCAAG TCCCAGTGCC 
GGCTCCAGTT CGCCAGTCAT CTCCGATCCG CACAACCGCC CGCCAAGCAC CACCATTGCC 
AATCTCCAGG TGCAACTGCA GCAGGCACTA AACCGCAATG TCAGGCCCGA GGATCTGGAC 
AGCTTGCGCA AGGTGGTGGC CGCCGGCGCT CTATACAATG CGGCAGCCGT GGTGGGAGCA 
CCACCACCGC CACCATCTGC TGGTCTCTAC GTGCCCGCTC CCAGCGCCTA TAAGGATCAC 
CTGGAAGCGG CTGCAGCCTG GAGCCACAAT GTCGAGGCTG CTGTGAGCAG CAGTGCTGTG 
GATGCGGTCA GTAGCTCATC GGTGTCCGGC AGTGCGGCCA GTGTGCTGAA TCTATCGCGT 
CGGGCCTGCT CACCCAGCTA CGAGCACATG CTCTCCTCCA CCACCTCCTC CACTCTTTCC 
TCGGCCTCCT CATCGGGAGC TGTTTCCGGC GATGATGAGC AGGAGCACGA ACCAGCGCAC 
ATGGCTCCAC TGCAGCTGCA GCGAAGCAGT CCGCAGCAGG GCAGCGATGC CAACAACTGT 
CTGCCCCTCA AGCTGCGCCA CAAGTCCCAT CTCGGCGACA AGGATGCGGC GGCCACGGCG 
CTACTCTCAC TGCAGCACAT CAAGCAGGAG CCCAACTGCA GTCGGGCATC GCCGCCGGCT 
TGGAACGATG GCGGCGACAA TTCCAGCGAC GAAAGGGACT CCGGCATCTC CATCGCCAGT 
GCGGAGTGGA CGGCGCAGCT GCAGAGGAAG CTACTGGCGC CCAAGGAGGC CAATGTGGTA 
ACCAGTGCCG AGCGCGATCA AATGCTCAAG TCGCAGCTGG AGCGCCTAGA GTCCGAGGTG 
GCCAGCATCA AGATGATCTT GGCGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137792.1
CDS	376..2565
Translation	MSIVCTLEQKVNFFKAAATTKNLLILKNNTDTNYINNYKQDNPSNNKFPRIQAQSNNSHLQHQQQLQQKLAQLHHYSQQKLSGSDFPYGPRPPTGGKEEKLLLLAPPGKLYPEASVSTAMPEVLSGTPTNSHNKANIAMMNNVRLSNISPTLSMNGSSNEASNLHPLSMYGGSISPQSNDSGMSDSLGKYVPGSGYGDGMMAQSPSQGGNGPQSALTAAQKELFSQRKQREFTPDNKKDESYWDRRRRNNEAAKRSREKRRYNDMVLEQRVIELTKENHVLKAQLDAIRDKFNISGENLVSVEKILASLPTSEQVLSNTKRAKMSGSGGSSSGSSPSGSGSGEGSPQGGHNGYPVGPPLSPLIYGPNGNARPEATVKSVHHIHHAGVAPPPTHLQQLVVPQSQTQHLYQPQPQQHQPHQQQQISQPPQQQQQQQEPSPSAGSSSPVISDPHNRPPSTTIANLQVQLQQALNRNVRPEDLDSLRKVVAAGALYNAAAVVGAPPPPPSAGLYVPAPSAYKDHLEAAAAWSHNVEAAVSSSAVDAVSSSSVSGSAASVLNLSRRACSPSYEHMLSSTTSSTLSSASSSGAVSGDDEQEHEPAHMAPLQLQRSSPQQGSDANNCLPLKLRHKSHLGDKDAAATALLSLQHIKQEPNCSRASPPAWNDGGDNSSDERDSGISIASAEWTAQLQRKLLAPKEANVVTSAERDQMLKSQLERLESEVASIKMILAE

Target ORF information:

RefSeq Version	NM_001144320.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vrille (vri), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144320.2

ORF Insert Sequence:

ATGTCGATTG TCTGCACGCT GGAACAGAAA GTGAACTTTT TCAAAGCGGC TGCAACGACC 
AAAAATCTGT TGATACTCAA AAACAATACG GATACGAATT ACATCAATAA CTACAAGCAG 
GATAATCCCA GCAACAATAA ATTTCCCCGC ATCCAAGCTC AAAGCAACAA CAGCCATCTG 
CAACACCAGC AGCAACTGCA GCAGAAACTA GCGCAGCTGC ATCACTACAG CCAGCAGAAG 
CTGAGCGGCA GCGACTTTCC CTACGGGCCA CGCCCCCCCA CCGGAGGCAA AGAGGAGAAG 
CTGCTCCTGC TAGCGCCGCC GGGCAAACTC TATCCGGAGG CGTCTGTGTC CACAGCAATG 
CCCGAGGTCC TCAGCGGCAC GCCCACCAAC AGCCACAACA AAGCCAACAT CGCCATGATG 
AACAACGTCC GGCTATCCAA TATATCGCCG ACTCTCTCGA TGAACGGCAG CTCCAACGAG 
GCTTCTAACT TGCATCCGTT GTCCATGTAC GGAGGATCCA TAAGTCCGCA GTCAAATGAC 
AGCGGCATGT CCGACAGTCT GGGCAAATAT GTCCCGGGAA GCGGCTATGG AGATGGAATG 
ATGGCCCAGT CGCCCTCACA GGGTGGCAAT GGACCGCAGT CGGCGTTGAC CGCCGCCCAG 
AAAGAGCTCT TCTCGCAGCG AAAGCAACGC GAATTCACCC CGGACAACAA GAAGGACGAG 
AGCTACTGGG ATCGCCGGAG AAGGAACAAC GAGGCGGCCA AGCGGAGTCG AGAGAAGAGG 
CGCTACAATG ACATGGTTCT GGAGCAGCGC GTCATCGAGC TGACCAAAGA GAACCATGTG 
CTGAAGGCCC AGCTGGACGC CATACGCGAC AAGTTCAACA TCTCCGGCGA GAATCTGGTG 
AGCGTGGAGA AGATCCTCGC CTCGCTGCCC ACCAGCGAAC AGGTGCTGAG TAACACAAAG 
CGCGCCAAGA TGAGCGGCAG TGGTGGCTCC TCCTCGGGCT CCTCGCCCTC GGGCAGTGGA 
TCGGGCGAGG GATCGCCTCA AGGTGGCCAC AATGGCTATC CGGTGGGTCC ACCACTATCT 
CCACTGATCT ACGGACCCAA TGGCAATGCC CGGCCAGAGG CCACGGTTAA GAGTGTCCAC 
CACATACATC ATGCGGGTGT TGCGCCGCCA CCAACGCACC TACAACAGTT GGTTGTGCCC 
CAGTCCCAGA CACAGCATCT CTACCAGCCA CAGCCGCAGC AGCATCAGCC GCATCAGCAG 
CAGCAGATTT CCCAGCCACC GCAGCAACAA CAGCAGCAGC AGGAGCCAAG TCCCAGTGCC 
GGCTCCAGTT CGCCAGTCAT CTCCGATCCG CACAACCGCC CGCCAAGCAC CACCATTGCC 
AATCTCCAGG TGCAACTGCA GCAGGCACTA AACCGCAATG TCAGGCCCGA GGATCTGGAC 
AGCTTGCGCA AGGTGGTGGC CGCCGGCGCT CTATACAATG CGGCAGCCGT GGTGGGAGCA 
CCACCACCGC CACCATCTGC TGGTCTCTAC GTGCCCGCTC CCAGCGCCTA TAAGGATCAC 
CTGGAAGCGG CTGCAGCCTG GAGCCACAAT GTCGAGGCTG CTGTGAGCAG CAGTGCTGTG 
GATGCGGTCA GTAGCTCATC GGTGTCCGGC AGTGCGGCCA GTGTGCTGAA TCTATCGCGT 
CGGGCCTGCT CACCCAGCTA CGAGCACATG CTCTCCTCCA CCACCTCCTC CACTCTTTCC 
TCGGCCTCCT CATCGGGAGC TGTTTCCGGC GATGATGAGC AGGAGCACGA ACCAGCGCAC 
ATGGCTCCAC TGCAGCTGCA GCGAAGCAGT CCGCAGCAGG GCAGCGATGC CAACAACTGT 
CTGCCCCTCA AGCTGCGCCA CAAGTCCCAT CTCGGCGACA AGGATGCGGC GGCCACGGCG 
CTACTCTCAC TGCAGCACAT CAAGCAGGAG CCCAACTGCA GTCGGGCATC GCCGCCGGCT 
TGGAACGATG GCGGCGACAA TTCCAGCGAC GAAAGGGACT CCGGCATCTC CATCGCCAGT 
GCGGAGTGGA CGGCGCAGCT GCAGAGGAAG CTACTGGCGC CCAAGGAGGC CAATGTGGTA 
ACCAGTGCCG AGCGCGATCA AATGCTCAAG TCGCAGCTGG AGCGCCTAGA GTCCGAGGTG 
GCCAGCATCA AGATGATCTT GGCGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vri ( NM_001298695.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285624.1 Drosophila melanogaster vrille (vri), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa13796
Clone ID Related Accession (Same CDS sequence)	NM_057843.4 , NM_001144320.2 , NM_001298695.1
Accession Version	NM_001298695.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vrille, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGATTG TCTGCACGCT GGAACAGAAA GTGAACTTTT TCAAAGCGGC TGCAACGACC 
AAAAATCTGT TGATACTCAA AAACAATACG GATACGAATT ACATCAATAA CTACAAGCAG 
GATAATCCCA GCAACAATAA ATTTCCCCGC ATCCAAGCTC AAAGCAACAA CAGCCATCTG 
CAACACCAGC AGCAACTGCA GCAGAAACTA GCGCAGCTGC ATCACTACAG CCAGCAGAAG 
CTGAGCGGCA GCGACTTTCC CTACGGGCCA CGCCCCCCCA CCGGAGGCAA AGAGGAGAAG 
CTGCTCCTGC TAGCGCCGCC GGGCAAACTC TATCCGGAGG CGTCTGTGTC CACAGCAATG 
CCCGAGGTCC TCAGCGGCAC GCCCACCAAC AGCCACAACA AAGCCAACAT CGCCATGATG 
AACAACGTCC GGCTATCCAA TATATCGCCG ACTCTCTCGA TGAACGGCAG CTCCAACGAG 
GCTTCTAACT TGCATCCGTT GTCCATGTAC GGAGGATCCA TAAGTCCGCA GTCAAATGAC 
AGCGGCATGT CCGACAGTCT GGGCAAATAT GTCCCGGGAA GCGGCTATGG AGATGGAATG 
ATGGCCCAGT CGCCCTCACA GGGTGGCAAT GGACCGCAGT CGGCGTTGAC CGCCGCCCAG 
AAAGAGCTCT TCTCGCAGCG AAAGCAACGC GAATTCACCC CGGACAACAA GAAGGACGAG 
AGCTACTGGG ATCGCCGGAG AAGGAACAAC GAGGCGGCCA AGCGGAGTCG AGAGAAGAGG 
CGCTACAATG ACATGGTTCT GGAGCAGCGC GTCATCGAGC TGACCAAAGA GAACCATGTG 
CTGAAGGCCC AGCTGGACGC CATACGCGAC AAGTTCAACA TCTCCGGCGA GAATCTGGTG 
AGCGTGGAGA AGATCCTCGC CTCGCTGCCC ACCAGCGAAC AGGTGCTGAG TAACACAAAG 
CGCGCCAAGA TGAGCGGCAG TGGTGGCTCC TCCTCGGGCT CCTCGCCCTC GGGCAGTGGA 
TCGGGCGAGG GATCGCCTCA AGGTGGCCAC AATGGCTATC CGGTGGGTCC ACCACTATCT 
CCACTGATCT ACGGACCCAA TGGCAATGCC CGGCCAGAGG CCACGGTTAA GAGTGTCCAC 
CACATACATC ATGCGGGTGT TGCGCCGCCA CCAACGCACC TACAACAGTT GGTTGTGCCC 
CAGTCCCAGA CACAGCATCT CTACCAGCCA CAGCCGCAGC AGCATCAGCC GCATCAGCAG 
CAGCAGATTT CCCAGCCACC GCAGCAACAA CAGCAGCAGC AGGAGCCAAG TCCCAGTGCC 
GGCTCCAGTT CGCCAGTCAT CTCCGATCCG CACAACCGCC CGCCAAGCAC CACCATTGCC 
AATCTCCAGG TGCAACTGCA GCAGGCACTA AACCGCAATG TCAGGCCCGA GGATCTGGAC 
AGCTTGCGCA AGGTGGTGGC CGCCGGCGCT CTATACAATG CGGCAGCCGT GGTGGGAGCA 
CCACCACCGC CACCATCTGC TGGTCTCTAC GTGCCCGCTC CCAGCGCCTA TAAGGATCAC 
CTGGAAGCGG CTGCAGCCTG GAGCCACAAT GTCGAGGCTG CTGTGAGCAG CAGTGCTGTG 
GATGCGGTCA GTAGCTCATC GGTGTCCGGC AGTGCGGCCA GTGTGCTGAA TCTATCGCGT 
CGGGCCTGCT CACCCAGCTA CGAGCACATG CTCTCCTCCA CCACCTCCTC CACTCTTTCC 
TCGGCCTCCT CATCGGGAGC TGTTTCCGGC GATGATGAGC AGGAGCACGA ACCAGCGCAC 
ATGGCTCCAC TGCAGCTGCA GCGAAGCAGT CCGCAGCAGG GCAGCGATGC CAACAACTGT 
CTGCCCCTCA AGCTGCGCCA CAAGTCCCAT CTCGGCGACA AGGATGCGGC GGCCACGGCG 
CTACTCTCAC TGCAGCACAT CAAGCAGGAG CCCAACTGCA GTCGGGCATC GCCGCCGGCT 
TGGAACGATG GCGGCGACAA TTCCAGCGAC GAAAGGGACT CCGGCATCTC CATCGCCAGT 
GCGGAGTGGA CGGCGCAGCT GCAGAGGAAG CTACTGGCGC CCAAGGAGGC CAATGTGGTA 
ACCAGTGCCG AGCGCGATCA AATGCTCAAG TCGCAGCTGG AGCGCCTAGA GTCCGAGGTG 
GCCAGCATCA AGATGATCTT GGCGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285624.1
CDS	376..2565
Translation	MSIVCTLEQKVNFFKAAATTKNLLILKNNTDTNYINNYKQDNPSNNKFPRIQAQSNNSHLQHQQQLQQKLAQLHHYSQQKLSGSDFPYGPRPPTGGKEEKLLLLAPPGKLYPEASVSTAMPEVLSGTPTNSHNKANIAMMNNVRLSNISPTLSMNGSSNEASNLHPLSMYGGSISPQSNDSGMSDSLGKYVPGSGYGDGMMAQSPSQGGNGPQSALTAAQKELFSQRKQREFTPDNKKDESYWDRRRRNNEAAKRSREKRRYNDMVLEQRVIELTKENHVLKAQLDAIRDKFNISGENLVSVEKILASLPTSEQVLSNTKRAKMSGSGGSSSGSSPSGSGSGEGSPQGGHNGYPVGPPLSPLIYGPNGNARPEATVKSVHHIHHAGVAPPPTHLQQLVVPQSQTQHLYQPQPQQHQPHQQQQISQPPQQQQQQQEPSPSAGSSSPVISDPHNRPPSTTIANLQVQLQQALNRNVRPEDLDSLRKVVAAGALYNAAAVVGAPPPPPSAGLYVPAPSAYKDHLEAAAAWSHNVEAAVSSSAVDAVSSSSVSGSAASVLNLSRRACSPSYEHMLSSTTSSTLSSASSSGAVSGDDEQEHEPAHMAPLQLQRSSPQQGSDANNCLPLKLRHKSHLGDKDAAATALLSLQHIKQEPNCSRASPPAWNDGGDNSSDERDSGISIASAEWTAQLQRKLLAPKEANVVTSAERDQMLKSQLERLESEVASIKMILAE

Target ORF information:

RefSeq Version	NM_001298695.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vrille (vri), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298695.1

ORF Insert Sequence:

ATGTCGATTG TCTGCACGCT GGAACAGAAA GTGAACTTTT TCAAAGCGGC TGCAACGACC 
AAAAATCTGT TGATACTCAA AAACAATACG GATACGAATT ACATCAATAA CTACAAGCAG 
GATAATCCCA GCAACAATAA ATTTCCCCGC ATCCAAGCTC AAAGCAACAA CAGCCATCTG 
CAACACCAGC AGCAACTGCA GCAGAAACTA GCGCAGCTGC ATCACTACAG CCAGCAGAAG 
CTGAGCGGCA GCGACTTTCC CTACGGGCCA CGCCCCCCCA CCGGAGGCAA AGAGGAGAAG 
CTGCTCCTGC TAGCGCCGCC GGGCAAACTC TATCCGGAGG CGTCTGTGTC CACAGCAATG 
CCCGAGGTCC TCAGCGGCAC GCCCACCAAC AGCCACAACA AAGCCAACAT CGCCATGATG 
AACAACGTCC GGCTATCCAA TATATCGCCG ACTCTCTCGA TGAACGGCAG CTCCAACGAG 
GCTTCTAACT TGCATCCGTT GTCCATGTAC GGAGGATCCA TAAGTCCGCA GTCAAATGAC 
AGCGGCATGT CCGACAGTCT GGGCAAATAT GTCCCGGGAA GCGGCTATGG AGATGGAATG 
ATGGCCCAGT CGCCCTCACA GGGTGGCAAT GGACCGCAGT CGGCGTTGAC CGCCGCCCAG 
AAAGAGCTCT TCTCGCAGCG AAAGCAACGC GAATTCACCC CGGACAACAA GAAGGACGAG 
AGCTACTGGG ATCGCCGGAG AAGGAACAAC GAGGCGGCCA AGCGGAGTCG AGAGAAGAGG 
CGCTACAATG ACATGGTTCT GGAGCAGCGC GTCATCGAGC TGACCAAAGA GAACCATGTG 
CTGAAGGCCC AGCTGGACGC CATACGCGAC AAGTTCAACA TCTCCGGCGA GAATCTGGTG 
AGCGTGGAGA AGATCCTCGC CTCGCTGCCC ACCAGCGAAC AGGTGCTGAG TAACACAAAG 
CGCGCCAAGA TGAGCGGCAG TGGTGGCTCC TCCTCGGGCT CCTCGCCCTC GGGCAGTGGA 
TCGGGCGAGG GATCGCCTCA AGGTGGCCAC AATGGCTATC CGGTGGGTCC ACCACTATCT 
CCACTGATCT ACGGACCCAA TGGCAATGCC CGGCCAGAGG CCACGGTTAA GAGTGTCCAC 
CACATACATC ATGCGGGTGT TGCGCCGCCA CCAACGCACC TACAACAGTT GGTTGTGCCC 
CAGTCCCAGA CACAGCATCT CTACCAGCCA CAGCCGCAGC AGCATCAGCC GCATCAGCAG 
CAGCAGATTT CCCAGCCACC GCAGCAACAA CAGCAGCAGC AGGAGCCAAG TCCCAGTGCC 
GGCTCCAGTT CGCCAGTCAT CTCCGATCCG CACAACCGCC CGCCAAGCAC CACCATTGCC 
AATCTCCAGG TGCAACTGCA GCAGGCACTA AACCGCAATG TCAGGCCCGA GGATCTGGAC 
AGCTTGCGCA AGGTGGTGGC CGCCGGCGCT CTATACAATG CGGCAGCCGT GGTGGGAGCA 
CCACCACCGC CACCATCTGC TGGTCTCTAC GTGCCCGCTC CCAGCGCCTA TAAGGATCAC 
CTGGAAGCGG CTGCAGCCTG GAGCCACAAT GTCGAGGCTG CTGTGAGCAG CAGTGCTGTG 
GATGCGGTCA GTAGCTCATC GGTGTCCGGC AGTGCGGCCA GTGTGCTGAA TCTATCGCGT 
CGGGCCTGCT CACCCAGCTA CGAGCACATG CTCTCCTCCA CCACCTCCTC CACTCTTTCC 
TCGGCCTCCT CATCGGGAGC TGTTTCCGGC GATGATGAGC AGGAGCACGA ACCAGCGCAC 
ATGGCTCCAC TGCAGCTGCA GCGAAGCAGT CCGCAGCAGG GCAGCGATGC CAACAACTGT 
CTGCCCCTCA AGCTGCGCCA CAAGTCCCAT CTCGGCGACA AGGATGCGGC GGCCACGGCG 
CTACTCTCAC TGCAGCACAT CAAGCAGGAG CCCAACTGCA GTCGGGCATC GCCGCCGGCT 
TGGAACGATG GCGGCGACAA TTCCAGCGAC GAAAGGGACT CCGGCATCTC CATCGCCAGT 
GCGGAGTGGA CGGCGCAGCT GCAGAGGAAG CTACTGGCGC CCAAGGAGGC CAATGTGGTA 
ACCAGTGCCG AGCGCGATCA AATGCTCAAG TCGCAGCTGG AGCGCCTAGA GTCCGAGGTG 
GCCAGCATCA AGATGATCTT GGCGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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vri cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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