CG12547 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG12547
Entrez Gene ID	3355145
Full Name	CG12547 gene product from transcript CG12547-RB
Synonyms	CG40277,Dmel\CG12547,anon-EST:Posey268
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 41C2-41C2

mRNA and Protein(s)

mRNA	Protein	Name
NM_001299207.1	NP_001286136.1	uncharacterized protein, isoform C
NM_001042995.2	NP_001036460.1	uncharacterized protein, isoform A
NM_001273775.1	NP_001260704.1	uncharacterized protein, isoform B

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NHLRC2	NP_001077192.1
Pan troglodytes (chimpanzee)	NHLRC2	XP_508046.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010610	XP_314572.4
Xenopus tropicalis (tropical clawed frog)	nhlrc2	XP_004919443.1
Canis lupus familiaris (dog)	NHLRC2	XP_544027.2
Drosophila melanogaster (fruit fly)	CG12547	NP_001036460.1
Arabidopsis thaliana (thale cress)	AT1G56500	NP_564718.2
Mus musculus (house mouse)	Nhlrc2	NP_080087.1
Homo sapiens (human)	NHLRC2	NP_940916.2
	NHLRC2	XP_001091193.1
Rattus norvegicus (Norway rat)	Nhlrc2	NP_001100914.1
Gallus gallus (chicken)	NHLRC2	NP_001006504.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG12547 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG12547 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa22376	NM_001299207.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant C (CG12547), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa11835	NM_001042995.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG12547), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa11835	NM_001273775.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG12547), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG12547 ( NM_001299207.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286136.1 Drosophila melanogaster uncharacterized protein, transcript variant C (CG12547), mRNA.

Price & Availability↑

CloneID	OFa22376
Clone ID Related Accession (Same CDS sequence)	NM_001299207.1
Accession Version	NM_001299207.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCATGTGC TGCAAGAGTT GCACTTATTG GAAGAACGCT TTCCAATAGA AAGTGGTGTT 
GTAGTAATCG GTGTTCACAG TCCTAAGTTT GAAAATGAAC GCAATGCGGC AAACATTCTG 
TCCGCTGTAC AACGATATGG AATATCACAT CCCATTGTCA ATGATTCACG ATCTGGAATG 
TGGCGCGCTC TTGGAATTCG TTGCTGGCCT TCTCTTTTGA TTCTAAGCCC GACTGGTGTT 
CCGATGATGT TATTAATGGG TGAAGGGCAT GGAAAATTCC TACAGGATTT TTTTTCTTCT 
GCCCTATCGT ATTTTAGCCG TCAGGGTATA ATTGACCATA GGGGTTTACC TTTAAAATTA 
TTTAATGACT TTCAACCAGC ATCTAACTTG CGTTTTCCCG CAAAGATTGT TCAAGGCCCT 
AATGGCCTGT ATGCTATTGC TGATACGGGG AACAACAGGG TGCTTGTGCT TACAGCAGGC 
GGTGTGGTAC AGCACAGAAT TGGAGGACAC CAGCCTGGTT TCGTGGATGG AGACCTAACA 
GTGGCACGGT TTAATAATCC TCAAGGCATT GCGTTTCTTA ATGAAAACGT TTTGATTGTA 
GCGGACACTA AAAACCACGC CATTCGTCAG ATCTCTCTAA CAAATGCAAT GGTGGAAACT 
CTTGCTGGCA CAGGAATTCA GGGAAATGAT CGAATAGGTG GACGTCTAGG ACCATTGCAA 
CCACTGTCGT CACCGTGGGA TGTTGCCATA TTTCGAACGA GGGACATGGA TATGTCCTTT 
CATCTTGATG AACGTAACAT ACCAGAAAAA ACAATTATCC TTATATCCAT GGCAGGCACC 
CACCAGATTT GGGGCTATTT TCCCGAAGGA ATTATTTGGT GGAAATTTCG TAAATTTGAG 
CCTTGTTGCT GTGTCTCACT AATAGGAAAT GGTTTGGAAG AGAACCGTAA TAATTCTTAC 
CCACAGAACG CTGCATTCGC ACAGCCATCT GGCCTGGCCA TAGCGGGAGA CGTTTTATAT 
ATAGCTGACA GCGAAAGCTC TAGCATACGC AAAGCTTCTA TGATAGATGG AAAGGTTATG 
CCTGTCGTCG GAGGAGATCG AAACCCACTT AATTTATTTG CATTTGGCGA CATTGACGGT 
AGACTATTCA GCGCGAAACT CCAACATCCG TTAGGAGTGA CTTTCAATGA TACCAATAAC 
AAACTCTATG TTGCTGATAC CTATAATCAT AAAATCAAAA TTATTGACAT CGATTCAAAT 
GATATATCTA CTCTACAAAT TAAGAGCCAA GAAAATACTA ACCTGATTTT AAACGAGCCC 
GCCGGACTGT GCTTGGATGC CAGCGGACGG AATTTGTTGG TAGCTGATAC CAATAACCAT 
TCAATACATA CAATAGATTT GGTAACGCTT ATAGCACAAC CGTTTGGCTT AGATTTCAGA 
CAAATAGCGT CCGCTAGTGA AATTGATGCG CCACAAGATA CACAAAAGCC TACGGAAAAT 
AATATAGTTA AAGCATTGCC TCTTGATTTA ATTAAACCAA GTAAAATTTT TTTTAACCTT 
CGGCTTTCGC CCAGATTTAA CTTTACAAAG GAAGCTCCTC AAAAATGGAT AGTGAAAACT 
GTATGTCAAT CTGTAATAGT AAATCCAACG TGCGGAAATC TACTTGATGG GATGTGCCAC 
ATGCAGGTGC AGGCTACTAG GCCTGATTTT ATGTGTGAAA GCAGTCAAAT ATTTACCATC 
GAATTCGTAT TAAATTTATG CCTTTCAAAT TGTTGCCTAG TAAAAAAAAT CACGGTTTCT 
ATAAAACGCG ATGACATACA AGAAGAATAT ATATCCATCC ACAACGTAAA TATTGACATT 
GAACAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286136.1
CDS	439..2307
Translation	MHVLQELHLLEERFPIESGVVVIGVHSPKFENERNAANILSAVQRYGISHPIVNDSRSGMWRALGIRCWPSLLILSPTGVPMMLLMGEGHGKFLQDFFSSALSYFSRQGIIDHRGLPLKLFNDFQPASNLRFPAKIVQGPNGLYAIADTGNNRVLVLTAGGVVQHRIGGHQPGFVDGDLTVARFNNPQGIAFLNENVLIVADTKNHAIRQISLTNAMVETLAGTGIQGNDRIGGRLGPLQPLSSPWDVAIFRTRDMDMSFHLDERNIPEKTIILISMAGTHQIWGYFPEGIIWWKFRKFEPCCCVSLIGNGLEENRNNSYPQNAAFAQPSGLAIAGDVLYIADSESSSIRKASMIDGKVMPVVGGDRNPLNLFAFGDIDGRLFSAKLQHPLGVTFNDTNNKLYVADTYNHKIKIIDIDSNDISTLQIKSQENTNLILNEPAGLCLDASGRNLLVADTNNHSIHTIDLVTLIAQPFGLDFRQIASASEIDAPQDTQKPTENNIVKALPLDLIKPSKIFFNLRLSPRFNFTKEAPQKWIVKTVCQSVIVNPTCGNLLDGMCHMQVQATRPDFMCESSQIFTIEFVLNLCLSNCCLVKKITVSIKRDDIQEEYISIHNVNIDIEQ

Target ORF information:

RefSeq Version	NM_001299207.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant C (CG12547), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299207.1

ORF Insert Sequence:

ATGCATGTGC TGCAAGAGTT GCACTTATTG GAAGAACGCT TTCCAATAGA AAGTGGTGTT 
GTAGTAATCG GTGTTCACAG TCCTAAGTTT GAAAATGAAC GCAATGCGGC AAACATTCTG 
TCCGCTGTAC AACGATATGG AATATCACAT CCCATTGTCA ATGATTCACG ATCTGGAATG 
TGGCGCGCTC TTGGAATTCG TTGCTGGCCT TCTCTTTTGA TTCTAAGCCC GACTGGTGTT 
CCGATGATGT TATTAATGGG TGAAGGGCAT GGAAAATTCC TACAGGATTT TTTTTCTTCT 
GCCCTATCGT ATTTTAGCCG TCAGGGTATA ATTGACCATA GGGGTTTACC TTTAAAATTA 
TTTAATGACT TTCAACCAGC ATCTAACTTG CGTTTTCCCG CAAAGATTGT TCAAGGCCCT 
AATGGCCTGT ATGCTATTGC TGATACGGGG AACAACAGGG TGCTTGTGCT TACAGCAGGC 
GGTGTGGTAC AGCACAGAAT TGGAGGACAC CAGCCTGGTT TCGTGGATGG AGACCTAACA 
GTGGCACGGT TTAATAATCC TCAAGGCATT GCGTTTCTTA ATGAAAACGT TTTGATTGTA 
GCGGACACTA AAAACCACGC CATTCGTCAG ATCTCTCTAA CAAATGCAAT GGTGGAAACT 
CTTGCTGGCA CAGGAATTCA GGGAAATGAT CGAATAGGTG GACGTCTAGG ACCATTGCAA 
CCACTGTCGT CACCGTGGGA TGTTGCCATA TTTCGAACGA GGGACATGGA TATGTCCTTT 
CATCTTGATG AACGTAACAT ACCAGAAAAA ACAATTATCC TTATATCCAT GGCAGGCACC 
CACCAGATTT GGGGCTATTT TCCCGAAGGA ATTATTTGGT GGAAATTTCG TAAATTTGAG 
CCTTGTTGCT GTGTCTCACT AATAGGAAAT GGTTTGGAAG AGAACCGTAA TAATTCTTAC 
CCACAGAACG CTGCATTCGC ACAGCCATCT GGCCTGGCCA TAGCGGGAGA CGTTTTATAT 
ATAGCTGACA GCGAAAGCTC TAGCATACGC AAAGCTTCTA TGATAGATGG AAAGGTTATG 
CCTGTCGTCG GAGGAGATCG AAACCCACTT AATTTATTTG CATTTGGCGA CATTGACGGT 
AGACTATTCA GCGCGAAACT CCAACATCCG TTAGGAGTGA CTTTCAATGA TACCAATAAC 
AAACTCTATG TTGCTGATAC CTATAATCAT AAAATCAAAA TTATTGACAT CGATTCAAAT 
GATATATCTA CTCTACAAAT TAAGAGCCAA GAAAATACTA ACCTGATTTT AAACGAGCCC 
GCCGGACTGT GCTTGGATGC CAGCGGACGG AATTTGTTGG TAGCTGATAC CAATAACCAT 
TCAATACATA CAATAGATTT GGTAACGCTT ATAGCACAAC CGTTTGGCTT AGATTTCAGA 
CAAATAGCGT CCGCTAGTGA AATTGATGCG CCACAAGATA CACAAAAGCC TACGGAAAAT 
AATATAGTTA AAGCATTGCC TCTTGATTTA ATTAAACCAA GTAAAATTTT TTTTAACCTT 
CGGCTTTCGC CCAGATTTAA CTTTACAAAG GAAGCTCCTC AAAAATGGAT AGTGAAAACT 
GTATGTCAAT CTGTAATAGT AAATCCAACG TGCGGAAATC TACTTGATGG GATGTGCCAC 
ATGCAGGTGC AGGCTACTAG GCCTGATTTT ATGTGTGAAA GCAGTCAAAT ATTTACCATC 
GAATTCGTAT TAAATTTATG CCTTTCAAAT TGTTGCCTAG TAAAAAAAAT CACGGTTTCT 
ATAAAACGCG ATGACATACA AGAAGAATAT ATATCCATCC ACAACGTAAA TATTGACATT 
GAACAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG12547 ( NM_001042995.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036460.1 Drosophila melanogaster uncharacterized protein, transcript variant A (CG12547), mRNA.

Price & Availability↑

CloneID	OFa11835
Clone ID Related Accession (Same CDS sequence)	NM_001042995.2 , NM_001273775.1
Accession Version	NM_001042995.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 16, 2013 this sequence version replaced NM_001042995.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATTTTA ATAATGACTT ATCGCCTATT GATATTTTGA CATTTATAAC AGATGAACTG 
TTGCAAGCAT ACAAAAGCAG TAAAGGAGAA AATGAAAAGG CTGGGACAAT TGTCAAGTTT 
CTTGACCGAT GGAATGACGA TATCTCCATT GCAAAACTCA AGGATCTCAC TGTTGAATTT 
GAATCAGATT TAGAGTGGTT CAATGTAAGT TGCCCCTTAT CGATAAAAGG ACTGCAAGGA 
AAAGTTATCA TATTAGACTT TTTCACATAT TGCTGCATTA ATTGTATGCA TGTGCTGCAA 
GAGTTGCACT TATTGGAAGA ACGCTTTCCA ATAGAAAGTG GTGTTGTAGT AATCGGTGTT 
CACAGTCCTA AGTTTGAAAA TGAACGCAAT GCGGCAAACA TTCTGTCCGC TGTACAACGA 
TATGGAATAT CACATCCCAT TGTCAATGAT TCACGATCTG GAATGTGGCG CGCTCTTGGA 
ATTCGTTGCT GGCCTTCTCT TTTGATTCTA AGCCCGACTG GTGTTCCGAT GATGTTATTA 
ATGGGTGAAG GGCATGGAAA ATTCCTACAG GATTTTTTTT CTTCTGCCCT ATCGTATTTT 
AGCCGTCAGG GTATAATTGA CCATAGGGGT TTACCTTTAA AATTATTTAA TGACTTTCAA 
CCAGCATCTA ACTTGCGTTT TCCCGCAAAG ATTGTTCAAG GCCCTAATGG CCTGTATGCT 
ATTGCTGATA CGGGGAACAA CAGGGTGCTT GTGCTTACAG CAGGCGGTGT GGTACAGCAC 
AGAATTGGAG GACACCAGCC TGGTTTCGTG GATGGAGACC TAACAGTGGC ACGGTTTAAT 
AATCCTCAAG GCATTGCGTT TCTTAATGAA AACGTTTTGA TTGTAGCGGA CACTAAAAAC 
CACGCCATTC GTCAGATCTC TCTAACAAAT GCAATGGTGG AAACTCTTGC TGGCACAGGA 
ATTCAGGGAA ATGATCGAAT AGGTGGACGT CTAGGACCAT TGCAACCACT GTCGTCACCG 
TGGGATGTTG CCATATTTCG AACGAGGGAC ATGGATATGT CCTTTCATCT TGATGAACGT 
AACATACCAG AAAAAACAAT TATCCTTATA TCCATGGCAG GCACCCACCA GATTTGGGGC 
TATTTTCCCG AAGGAATTAT TTGGTGGAAA TTTCGTAAAT TTGAGCCTTG TTGCTGTGTC 
TCACTAATAG GAAATGGTTT GGAAGAGAAC CGTAATAATT CTTACCCACA GAACGCTGCA 
TTCGCACAGC CATCTGGCCT GGCCATAGCG GGAGACGTTT TATATATAGC TGACAGCGAA 
AGCTCTAGCA TACGCAAAGC TTCTATGATA GATGGAAAGG TTATGCCTGT CGTCGGAGGA 
GATCGAAACC CACTTAATTT ATTTGCATTT GGCGACATTG ACGGTAGACT ATTCAGCGCG 
AAACTCCAAC ATCCGTTAGG AGTGACTTTC AATGATACCA ATAACAAACT CTATGTTGCT 
GATACCTATA ATCATAAAAT CAAAATTATT GACATCGATT CAAATGATAT ATCTACTCTA 
CAAATTAAGA GCCAAGAAAA TACTAACCTG ATTTTAAACG AGCCCGCCGG ACTGTGCTTG 
GATGCCAGCG GACGGAATTT GTTGGTAGCT GATACCAATA ACCATTCAAT ACATACAATA 
GATTTGGTAA CGCTTATAGC ACAACCGTTT GGCTTAGATT TCAGACAAAT AGCGTCCGCT 
AGTGAAATTG ATGCGCCACA AGATACACAA AAGCCTACGG AAAATAATAT AGTTAAAGCA 
TTGCCTCTTG ATTTAATTAA ACCAAGTAAA ATTTTTTTTA ACCTTCGGCT TTCGCCCAGA 
TTTAACTTTA CAAAGGAAGC TCCTCAAAAA TGGATAGTGA AAACTGTATG TCAATCTGTA 
ATAGTAAATC CAACGTGCGG AAATCTACTT GATGGGATGT GCCACATGCA GGTGCAGGCT 
ACTAGGCCTG ATTTTATGTG TGAAAGCAGT CAAATATTTA CCATCGAATT CGTATTAAAT 
TTATGCCTTT CAAATTGTTG CCTAGTAAAA AAAATCACGG TTTCTATAAA ACGCGATGAC 
ATACAAGAAG AATATATATC CATCCACAAC GTAAATATTG ACATTGAACA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036460.1
CDS	57..2210
Translation	MDFNNDLSPIDILTFITDELLQAYKSSKGENEKAGTIVKFLDRWNDDISIAKLKDLTVEFESDLEWFNVSCPLSIKGLQGKVIILDFFTYCCINCMHVLQELHLLEERFPIESGVVVIGVHSPKFENERNAANILSAVQRYGISHPIVNDSRSGMWRALGIRCWPSLLILSPTGVPMMLLMGEGHGKFLQDFFSSALSYFSRQGIIDHRGLPLKLFNDFQPASNLRFPAKIVQGPNGLYAIADTGNNRVLVLTAGGVVQHRIGGHQPGFVDGDLTVARFNNPQGIAFLNENVLIVADTKNHAIRQISLTNAMVETLAGTGIQGNDRIGGRLGPLQPLSSPWDVAIFRTRDMDMSFHLDERNIPEKTIILISMAGTHQIWGYFPEGIIWWKFRKFEPCCCVSLIGNGLEENRNNSYPQNAAFAQPSGLAIAGDVLYIADSESSSIRKASMIDGKVMPVVGGDRNPLNLFAFGDIDGRLFSAKLQHPLGVTFNDTNNKLYVADTYNHKIKIIDIDSNDISTLQIKSQENTNLILNEPAGLCLDASGRNLLVADTNNHSIHTIDLVTLIAQPFGLDFRQIASASEIDAPQDTQKPTENNIVKALPLDLIKPSKIFFNLRLSPRFNFTKEAPQKWIVKTVCQSVIVNPTCGNLLDGMCHMQVQATRPDFMCESSQIFTIEFVLNLCLSNCCLVKKITVSIKRDDIQEEYISIHNVNIDIEQ

Target ORF information:

RefSeq Version	NM_001042995.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG12547), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042995.2

ORF Insert Sequence:

ATGGATTTTA ATAATGACTT ATCGCCTATT GATATTTTGA CATTTATAAC AGATGAACTG 
TTGCAAGCAT ACAAAAGCAG TAAAGGAGAA AATGAAAAGG CTGGGACAAT TGTCAAGTTT 
CTTGACCGAT GGAATGACGA TATCTCCATT GCAAAACTCA AGGATCTCAC TGTTGAATTT 
GAATCAGATT TAGAGTGGTT CAATGTAAGT TGCCCCTTAT CGATAAAAGG ACTGCAAGGA 
AAAGTTATCA TATTAGACTT TTTCACATAT TGCTGCATTA ATTGTATGCA TGTGCTGCAA 
GAGTTGCACT TATTGGAAGA ACGCTTTCCA ATAGAAAGTG GTGTTGTAGT AATCGGTGTT 
CACAGTCCTA AGTTTGAAAA TGAACGCAAT GCGGCAAACA TTCTGTCCGC TGTACAACGA 
TATGGAATAT CACATCCCAT TGTCAATGAT TCACGATCTG GAATGTGGCG CGCTCTTGGA 
ATTCGTTGCT GGCCTTCTCT TTTGATTCTA AGCCCGACTG GTGTTCCGAT GATGTTATTA 
ATGGGTGAAG GGCATGGAAA ATTCCTACAG GATTTTTTTT CTTCTGCCCT ATCGTATTTT 
AGCCGTCAGG GTATAATTGA CCATAGGGGT TTACCTTTAA AATTATTTAA TGACTTTCAA 
CCAGCATCTA ACTTGCGTTT TCCCGCAAAG ATTGTTCAAG GCCCTAATGG CCTGTATGCT 
ATTGCTGATA CGGGGAACAA CAGGGTGCTT GTGCTTACAG CAGGCGGTGT GGTACAGCAC 
AGAATTGGAG GACACCAGCC TGGTTTCGTG GATGGAGACC TAACAGTGGC ACGGTTTAAT 
AATCCTCAAG GCATTGCGTT TCTTAATGAA AACGTTTTGA TTGTAGCGGA CACTAAAAAC 
CACGCCATTC GTCAGATCTC TCTAACAAAT GCAATGGTGG AAACTCTTGC TGGCACAGGA 
ATTCAGGGAA ATGATCGAAT AGGTGGACGT CTAGGACCAT TGCAACCACT GTCGTCACCG 
TGGGATGTTG CCATATTTCG AACGAGGGAC ATGGATATGT CCTTTCATCT TGATGAACGT 
AACATACCAG AAAAAACAAT TATCCTTATA TCCATGGCAG GCACCCACCA GATTTGGGGC 
TATTTTCCCG AAGGAATTAT TTGGTGGAAA TTTCGTAAAT TTGAGCCTTG TTGCTGTGTC 
TCACTAATAG GAAATGGTTT GGAAGAGAAC CGTAATAATT CTTACCCACA GAACGCTGCA 
TTCGCACAGC CATCTGGCCT GGCCATAGCG GGAGACGTTT TATATATAGC TGACAGCGAA 
AGCTCTAGCA TACGCAAAGC TTCTATGATA GATGGAAAGG TTATGCCTGT CGTCGGAGGA 
GATCGAAACC CACTTAATTT ATTTGCATTT GGCGACATTG ACGGTAGACT ATTCAGCGCG 
AAACTCCAAC ATCCGTTAGG AGTGACTTTC AATGATACCA ATAACAAACT CTATGTTGCT 
GATACCTATA ATCATAAAAT CAAAATTATT GACATCGATT CAAATGATAT ATCTACTCTA 
CAAATTAAGA GCCAAGAAAA TACTAACCTG ATTTTAAACG AGCCCGCCGG ACTGTGCTTG 
GATGCCAGCG GACGGAATTT GTTGGTAGCT GATACCAATA ACCATTCAAT ACATACAATA 
GATTTGGTAA CGCTTATAGC ACAACCGTTT GGCTTAGATT TCAGACAAAT AGCGTCCGCT 
AGTGAAATTG ATGCGCCACA AGATACACAA AAGCCTACGG AAAATAATAT AGTTAAAGCA 
TTGCCTCTTG ATTTAATTAA ACCAAGTAAA ATTTTTTTTA ACCTTCGGCT TTCGCCCAGA 
TTTAACTTTA CAAAGGAAGC TCCTCAAAAA TGGATAGTGA AAACTGTATG TCAATCTGTA 
ATAGTAAATC CAACGTGCGG AAATCTACTT GATGGGATGT GCCACATGCA GGTGCAGGCT 
ACTAGGCCTG ATTTTATGTG TGAAAGCAGT CAAATATTTA CCATCGAATT CGTATTAAAT 
TTATGCCTTT CAAATTGTTG CCTAGTAAAA AAAATCACGG TTTCTATAAA ACGCGATGAC 
ATACAAGAAG AATATATATC CATCCACAAC GTAAATATTG ACATTGAACA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG12547 ( NM_001273775.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260704.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG12547), mRNA.

Price & Availability↑

CloneID	OFa11835
Clone ID Related Accession (Same CDS sequence)	NM_001042995.2 , NM_001273775.1
Accession Version	NM_001273775.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATTTTA ATAATGACTT ATCGCCTATT GATATTTTGA CATTTATAAC AGATGAACTG 
TTGCAAGCAT ACAAAAGCAG TAAAGGAGAA AATGAAAAGG CTGGGACAAT TGTCAAGTTT 
CTTGACCGAT GGAATGACGA TATCTCCATT GCAAAACTCA AGGATCTCAC TGTTGAATTT 
GAATCAGATT TAGAGTGGTT CAATGTAAGT TGCCCCTTAT CGATAAAAGG ACTGCAAGGA 
AAAGTTATCA TATTAGACTT TTTCACATAT TGCTGCATTA ATTGTATGCA TGTGCTGCAA 
GAGTTGCACT TATTGGAAGA ACGCTTTCCA ATAGAAAGTG GTGTTGTAGT AATCGGTGTT 
CACAGTCCTA AGTTTGAAAA TGAACGCAAT GCGGCAAACA TTCTGTCCGC TGTACAACGA 
TATGGAATAT CACATCCCAT TGTCAATGAT TCACGATCTG GAATGTGGCG CGCTCTTGGA 
ATTCGTTGCT GGCCTTCTCT TTTGATTCTA AGCCCGACTG GTGTTCCGAT GATGTTATTA 
ATGGGTGAAG GGCATGGAAA ATTCCTACAG GATTTTTTTT CTTCTGCCCT ATCGTATTTT 
AGCCGTCAGG GTATAATTGA CCATAGGGGT TTACCTTTAA AATTATTTAA TGACTTTCAA 
CCAGCATCTA ACTTGCGTTT TCCCGCAAAG ATTGTTCAAG GCCCTAATGG CCTGTATGCT 
ATTGCTGATA CGGGGAACAA CAGGGTGCTT GTGCTTACAG CAGGCGGTGT GGTACAGCAC 
AGAATTGGAG GACACCAGCC TGGTTTCGTG GATGGAGACC TAACAGTGGC ACGGTTTAAT 
AATCCTCAAG GCATTGCGTT TCTTAATGAA AACGTTTTGA TTGTAGCGGA CACTAAAAAC 
CACGCCATTC GTCAGATCTC TCTAACAAAT GCAATGGTGG AAACTCTTGC TGGCACAGGA 
ATTCAGGGAA ATGATCGAAT AGGTGGACGT CTAGGACCAT TGCAACCACT GTCGTCACCG 
TGGGATGTTG CCATATTTCG AACGAGGGAC ATGGATATGT CCTTTCATCT TGATGAACGT 
AACATACCAG AAAAAACAAT TATCCTTATA TCCATGGCAG GCACCCACCA GATTTGGGGC 
TATTTTCCCG AAGGAATTAT TTGGTGGAAA TTTCGTAAAT TTGAGCCTTG TTGCTGTGTC 
TCACTAATAG GAAATGGTTT GGAAGAGAAC CGTAATAATT CTTACCCACA GAACGCTGCA 
TTCGCACAGC CATCTGGCCT GGCCATAGCG GGAGACGTTT TATATATAGC TGACAGCGAA 
AGCTCTAGCA TACGCAAAGC TTCTATGATA GATGGAAAGG TTATGCCTGT CGTCGGAGGA 
GATCGAAACC CACTTAATTT ATTTGCATTT GGCGACATTG ACGGTAGACT ATTCAGCGCG 
AAACTCCAAC ATCCGTTAGG AGTGACTTTC AATGATACCA ATAACAAACT CTATGTTGCT 
GATACCTATA ATCATAAAAT CAAAATTATT GACATCGATT CAAATGATAT ATCTACTCTA 
CAAATTAAGA GCCAAGAAAA TACTAACCTG ATTTTAAACG AGCCCGCCGG ACTGTGCTTG 
GATGCCAGCG GACGGAATTT GTTGGTAGCT GATACCAATA ACCATTCAAT ACATACAATA 
GATTTGGTAA CGCTTATAGC ACAACCGTTT GGCTTAGATT TCAGACAAAT AGCGTCCGCT 
AGTGAAATTG ATGCGCCACA AGATACACAA AAGCCTACGG AAAATAATAT AGTTAAAGCA 
TTGCCTCTTG ATTTAATTAA ACCAAGTAAA ATTTTTTTTA ACCTTCGGCT TTCGCCCAGA 
TTTAACTTTA CAAAGGAAGC TCCTCAAAAA TGGATAGTGA AAACTGTATG TCAATCTGTA 
ATAGTAAATC CAACGTGCGG AAATCTACTT GATGGGATGT GCCACATGCA GGTGCAGGCT 
ACTAGGCCTG ATTTTATGTG TGAAAGCAGT CAAATATTTA CCATCGAATT CGTATTAAAT 
TTATGCCTTT CAAATTGTTG CCTAGTAAAA AAAATCACGG TTTCTATAAA ACGCGATGAC 
ATACAAGAAG AATATATATC CATCCACAAC GTAAATATTG ACATTGAACA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260704.1
CDS	57..2210
Translation	MDFNNDLSPIDILTFITDELLQAYKSSKGENEKAGTIVKFLDRWNDDISIAKLKDLTVEFESDLEWFNVSCPLSIKGLQGKVIILDFFTYCCINCMHVLQELHLLEERFPIESGVVVIGVHSPKFENERNAANILSAVQRYGISHPIVNDSRSGMWRALGIRCWPSLLILSPTGVPMMLLMGEGHGKFLQDFFSSALSYFSRQGIIDHRGLPLKLFNDFQPASNLRFPAKIVQGPNGLYAIADTGNNRVLVLTAGGVVQHRIGGHQPGFVDGDLTVARFNNPQGIAFLNENVLIVADTKNHAIRQISLTNAMVETLAGTGIQGNDRIGGRLGPLQPLSSPWDVAIFRTRDMDMSFHLDERNIPEKTIILISMAGTHQIWGYFPEGIIWWKFRKFEPCCCVSLIGNGLEENRNNSYPQNAAFAQPSGLAIAGDVLYIADSESSSIRKASMIDGKVMPVVGGDRNPLNLFAFGDIDGRLFSAKLQHPLGVTFNDTNNKLYVADTYNHKIKIIDIDSNDISTLQIKSQENTNLILNEPAGLCLDASGRNLLVADTNNHSIHTIDLVTLIAQPFGLDFRQIASASEIDAPQDTQKPTENNIVKALPLDLIKPSKIFFNLRLSPRFNFTKEAPQKWIVKTVCQSVIVNPTCGNLLDGMCHMQVQATRPDFMCESSQIFTIEFVLNLCLSNCCLVKKITVSIKRDDIQEEYISIHNVNIDIEQ

Target ORF information:

RefSeq Version	NM_001273775.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG12547), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273775.1

ORF Insert Sequence:

ATGGATTTTA ATAATGACTT ATCGCCTATT GATATTTTGA CATTTATAAC AGATGAACTG 
TTGCAAGCAT ACAAAAGCAG TAAAGGAGAA AATGAAAAGG CTGGGACAAT TGTCAAGTTT 
CTTGACCGAT GGAATGACGA TATCTCCATT GCAAAACTCA AGGATCTCAC TGTTGAATTT 
GAATCAGATT TAGAGTGGTT CAATGTAAGT TGCCCCTTAT CGATAAAAGG ACTGCAAGGA 
AAAGTTATCA TATTAGACTT TTTCACATAT TGCTGCATTA ATTGTATGCA TGTGCTGCAA 
GAGTTGCACT TATTGGAAGA ACGCTTTCCA ATAGAAAGTG GTGTTGTAGT AATCGGTGTT 
CACAGTCCTA AGTTTGAAAA TGAACGCAAT GCGGCAAACA TTCTGTCCGC TGTACAACGA 
TATGGAATAT CACATCCCAT TGTCAATGAT TCACGATCTG GAATGTGGCG CGCTCTTGGA 
ATTCGTTGCT GGCCTTCTCT TTTGATTCTA AGCCCGACTG GTGTTCCGAT GATGTTATTA 
ATGGGTGAAG GGCATGGAAA ATTCCTACAG GATTTTTTTT CTTCTGCCCT ATCGTATTTT 
AGCCGTCAGG GTATAATTGA CCATAGGGGT TTACCTTTAA AATTATTTAA TGACTTTCAA 
CCAGCATCTA ACTTGCGTTT TCCCGCAAAG ATTGTTCAAG GCCCTAATGG CCTGTATGCT 
ATTGCTGATA CGGGGAACAA CAGGGTGCTT GTGCTTACAG CAGGCGGTGT GGTACAGCAC 
AGAATTGGAG GACACCAGCC TGGTTTCGTG GATGGAGACC TAACAGTGGC ACGGTTTAAT 
AATCCTCAAG GCATTGCGTT TCTTAATGAA AACGTTTTGA TTGTAGCGGA CACTAAAAAC 
CACGCCATTC GTCAGATCTC TCTAACAAAT GCAATGGTGG AAACTCTTGC TGGCACAGGA 
ATTCAGGGAA ATGATCGAAT AGGTGGACGT CTAGGACCAT TGCAACCACT GTCGTCACCG 
TGGGATGTTG CCATATTTCG AACGAGGGAC ATGGATATGT CCTTTCATCT TGATGAACGT 
AACATACCAG AAAAAACAAT TATCCTTATA TCCATGGCAG GCACCCACCA GATTTGGGGC 
TATTTTCCCG AAGGAATTAT TTGGTGGAAA TTTCGTAAAT TTGAGCCTTG TTGCTGTGTC 
TCACTAATAG GAAATGGTTT GGAAGAGAAC CGTAATAATT CTTACCCACA GAACGCTGCA 
TTCGCACAGC CATCTGGCCT GGCCATAGCG GGAGACGTTT TATATATAGC TGACAGCGAA 
AGCTCTAGCA TACGCAAAGC TTCTATGATA GATGGAAAGG TTATGCCTGT CGTCGGAGGA 
GATCGAAACC CACTTAATTT ATTTGCATTT GGCGACATTG ACGGTAGACT ATTCAGCGCG 
AAACTCCAAC ATCCGTTAGG AGTGACTTTC AATGATACCA ATAACAAACT CTATGTTGCT 
GATACCTATA ATCATAAAAT CAAAATTATT GACATCGATT CAAATGATAT ATCTACTCTA 
CAAATTAAGA GCCAAGAAAA TACTAACCTG ATTTTAAACG AGCCCGCCGG ACTGTGCTTG 
GATGCCAGCG GACGGAATTT GTTGGTAGCT GATACCAATA ACCATTCAAT ACATACAATA 
GATTTGGTAA CGCTTATAGC ACAACCGTTT GGCTTAGATT TCAGACAAAT AGCGTCCGCT 
AGTGAAATTG ATGCGCCACA AGATACACAA AAGCCTACGG AAAATAATAT AGTTAAAGCA 
TTGCCTCTTG ATTTAATTAA ACCAAGTAAA ATTTTTTTTA ACCTTCGGCT TTCGCCCAGA 
TTTAACTTTA CAAAGGAAGC TCCTCAAAAA TGGATAGTGA AAACTGTATG TCAATCTGTA 
ATAGTAAATC CAACGTGCGG AAATCTACTT GATGGGATGT GCCACATGCA GGTGCAGGCT 
ACTAGGCCTG ATTTTATGTG TGAAAGCAGT CAAATATTTA CCATCGAATT CGTATTAAAT 
TTATGCCTTT CAAATTGTTG CCTAGTAAAA AAAATCACGG TTTCTATAAA ACGCGATGAC 
ATACAAGAAG AATATATATC CATCCACAAC GTAAATATTG ACATTGAACA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG12547 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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