daw cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	daw
Entrez Gene ID	33474
Full Name	dawdle
Synonyms	ALP23B,Alp,Alp23B,Alp23b,CG16987,DAW,Daw,Dmel\CG16987,cg16987
General protein information	Preferred Names dawdle Names CG16987 gene product from transcript CG16987-RC Activin-Like protein CG16987-PA CG16987-PB CG16987-PC activin like protein activin like protein at 23B dActivin2 daw-PA daw-PB daw-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 23B1-23B2

mRNA and Protein(s)

mRNA	Protein	Name
NM_164503.2	NP_722840.1	dawdle, isoform B
NM_001273038.1	NP_001259967.1	dawdle, isoform C
NM_078737.3	NP_523461.1	dawdle, isoform A

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC100329520	XP_002662616.2
Drosophila melanogaster (fruit fly)	daw	NP_001259967.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Loss of Dawdle causes major alterations in larval metabolic activity, including accumulation of tricarboxylic acid cycle intermediates, acidification of hemolymph, and misregulation of insulin signaling and nuclear-encoded mitochondrial gene expression.
Title: Systemic Activin signaling independently regulates sugar homeostasis, cellular metabolism, and pH balance in Drosophila melanogaster.

dawdle is activated by Gram-positive bacterial infection but repressed by Gram-negative infection or wounding; its role is to limit infection-induced melanization. Flies lacking dawdle function exhibit melanization even when uninfected.
Title: Multiple TGF-? superfamily signals modulate the adult Drosophila immune response.

Study shows that the Daw/Babo pathway acts upstream of gbb, and is involved in maintenance of muscle gbb expression.
Title: Activin signaling functions upstream of Gbb to regulate synaptic growth at the Drosophila neuromuscular junction.

Dawdle, one of four Activin-type ligands in Drosophila, preferentially signals through Babo(c), one of three isoforms of the Activin Type-I receptor that are expressed during development.
Title: The Drosophila Activin-like ligand Dawdle signals preferentially through one isoform of the Type-I receptor Baboon.

These results suggest that the Activin signaling pathway is required for producing the proper number of neurons to enable normal connection of incoming photoreceptor axons to their targets.
Title: Drosophila Activin- and the Activin-like product Dawdle function redundantly to regulate proliferation in the larval brain.

The following daw gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the daw cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa13547	NM_001273038.1 Latest version!	Drosophila melanogaster dawdle (daw), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa13547	NM_164503.2 Latest version!	Drosophila melanogaster dawdle (daw), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa13547	NM_078737.3 Latest version!	Drosophila melanogaster dawdle (daw), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

daw ( NM_164503.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_722840.1 Drosophila melanogaster dawdle (daw), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa13547
Clone ID Related Accession (Same CDS sequence)	NM_164503.2 , NM_001273038.1 , NM_078737.3
Accession Version	NM_164503.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dawdle, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164503.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCAAGT ACTTTATAGC CCTGGTGTTG CTGGTGTGTC TGGCCCTGGA GAACAGGAAC 
GTATACAATC GCCTGCACGC CCGCTCCCAT CCGAGTTCCC GTGGCGACAT CCTTCGTCCG 
CATCCTAAGC ACCAGCCGCA TCCCCATGTG CAGCACACCT CGCAACAGCT GCAGCAGCAA 
CACCATATCC GGCAGCAACG ATCCCGTCAG CTGAAACACC TGGAACCGGA TCCGACCAGC 
GAGGAGGACG ATGTACCGAT CACCAAGCGG GAGTACTATG CCCGCCTGAG GCGCCACGTC 
ATCCACAAGA GGCAGCACCT CCTGCAGCAG GAGCTCAGCT ACAACCATCC CGGCTCCCAT 
TCGGAGCAGC TAAAGGTGCC CCGCCTGTGG CAACACCTCA TGGAGCAGGA GGAGAAGGCA 
CACCGACATG TGAGCCCGCC CGTGGAGTTG CCTATCATGT ACGGAGATGC CCCCGAGGAG 
TCATCCCTCT CCTCCGATGA TCACTTTGAT GATCTGTCGC CGCTGGACTT TGTGCGCAAC 
GAACTGATGG CCGAGGACCA GCAGAATCAG GAGAAGGATC TCGATCTCGA TCTGGACCTA 
GATCAGCAGC CGGAAACGCC CATAGAGCCA GAGCTCCCAT TGGGTGAGAG GAACATCACC 
ATTTCGGTGA AGTCCAGCGC CGGAGGATGC CCCAAGTGCG AGAGCAATCG CCAAGTGGAG 
CACATCACCG AGGAGCAGCT GACCCACTTA CGCATCGAGT TCGTTAAGCA GCAGATCCTG 
GAGAAGCTGC GCCTCAAGGA GAGCCCCAAG GTGTCGGCCG TGGAGCTGCC CAAGCCCATC 
TTCGACGGGA TGACACTCTC CCATCCGGAC GATAGCACCA AGAACAAGGA GCTGGACGAT 
TACTACGCTC GCACTAGCAA GAAGTTCATA CTGCTCAATA GAGAGGAGGT CGAGTGCAAC 
CGAGCGAGAG ATGGAAAATC CAATCCGTCC ATGTGCTTCA CCTTCAAGAT AGACGACGCA 
GATGCCGAGG GCTTCGACGT GAGCACCGCC GTCCTGTGGC TCTTCAAGAA CAAACAGAAC 
CGCACCGACA CGGCATCGGT GAACAGCACC AGTGCCCAGC AGACGATCGT AGTCTCCGAG 
GTGGAGGTGG ATCAGCAGAA GGACTCCAAG TATTTGTCGG CGGCCAAGAC CATAGCCATC 
CAGTCGGTGA ATGTGCAAGA TGAGTGGATG AAGATCGACA TTGAGTGGCC CATCAAACAC 
TGGATCAGTG GCCACGAGCT GAGCCACCTC ATCCAAATCA CCTGCGGTGG CTGCGATGTC 
AGCGACATGG AGGAGATCAT TTCGGTGGAC AAGGATTACC GGCCATTCAT CGTGATCGAC 
ATGCAGAACA GGCGGCGCAA GAGTCGCCAG AAGCGCAGCA TCAACTGCTC CAGCGGAATG 
ACCGAGTGCT GTCGGGAGCA CCTGTACATA TCATTCCGGG ACATCGGCTG GAGCAACTGG 
ATCCTGAAGC CGGAGGGCTA CAATGCCTAC TTCTGTCGCG GATCCTGTAG CTCGGTGGCG 
AGTGTTACGC AAGCGGCCTC CCACCACAGT TCCATAATGA AAATTCTCTC CACGAGCGGT 
GCTAACAAGT CGCTGGAGTT GGTGCCCTGC TGCACCGCCA AACAGTATTC CTCGCTGCAG 
CTGGTCGTGA TGGACTCGAG CAACACGGCG ACGGTGAAGA CGCTGCCCAA CATGGTGGTG 
GAGTCATGTG GCTGCAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_722840.1
CDS	673..2433
Translation	MAKYFIALVLLVCLALENRNVYNRLHARSHPSSRGDILRPHPKHQPHPHVQHTSQQLQQQHHIRQQRSRQLKHLEPDPTSEEDDVPITKREYYARLRRHVIHKRQHLLQQELSYNHPGSHSEQLKVPRLWQHLMEQEEKAHRHVSPPVELPIMYGDAPEESSLSSDDHFDDLSPLDFVRNELMAEDQQNQEKDLDLDLDLDQQPETPIEPELPLGERNITISVKSSAGGCPKCESNRQVEHITEEQLTHLRIEFVKQQILEKLRLKESPKVSAVELPKPIFDGMTLSHPDDSTKNKELDDYYARTSKKFILLNREEVECNRARDGKSNPSMCFTFKIDDADAEGFDVSTAVLWLFKNKQNRTDTASVNSTSAQQTIVVSEVEVDQQKDSKYLSAAKTIAIQSVNVQDEWMKIDIEWPIKHWISGHELSHLIQITCGGCDVSDMEEIISVDKDYRPFIVIDMQNRRRKSRQKRSINCSSGMTECCREHLYISFRDIGWSNWILKPEGYNAYFCRGSCSSVASVTQAASHHSSIMKILSTSGANKSLELVPCCTAKQYSSLQLVVMDSSNTATVKTLPNMVVESCGCR

Target ORF information:

RefSeq Version	NM_164503.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dawdle (daw), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164503.2

ORF Insert Sequence:

ATGGCCAAGT ACTTTATAGC CCTGGTGTTG CTGGTGTGTC TGGCCCTGGA GAACAGGAAC 
GTATACAATC GCCTGCACGC CCGCTCCCAT CCGAGTTCCC GTGGCGACAT CCTTCGTCCG 
CATCCTAAGC ACCAGCCGCA TCCCCATGTG CAGCACACCT CGCAACAGCT GCAGCAGCAA 
CACCATATCC GGCAGCAACG ATCCCGTCAG CTGAAACACC TGGAACCGGA TCCGACCAGC 
GAGGAGGACG ATGTACCGAT CACCAAGCGG GAGTACTATG CCCGCCTGAG GCGCCACGTC 
ATCCACAAGA GGCAGCACCT CCTGCAGCAG GAGCTCAGCT ACAACCATCC CGGCTCCCAT 
TCGGAGCAGC TAAAGGTGCC CCGCCTGTGG CAACACCTCA TGGAGCAGGA GGAGAAGGCA 
CACCGACATG TGAGCCCGCC CGTGGAGTTG CCTATCATGT ACGGAGATGC CCCCGAGGAG 
TCATCCCTCT CCTCCGATGA TCACTTTGAT GATCTGTCGC CGCTGGACTT TGTGCGCAAC 
GAACTGATGG CCGAGGACCA GCAGAATCAG GAGAAGGATC TCGATCTCGA TCTGGACCTA 
GATCAGCAGC CGGAAACGCC CATAGAGCCA GAGCTCCCAT TGGGTGAGAG GAACATCACC 
ATTTCGGTGA AGTCCAGCGC CGGAGGATGC CCCAAGTGCG AGAGCAATCG CCAAGTGGAG 
CACATCACCG AGGAGCAGCT GACCCACTTA CGCATCGAGT TCGTTAAGCA GCAGATCCTG 
GAGAAGCTGC GCCTCAAGGA GAGCCCCAAG GTGTCGGCCG TGGAGCTGCC CAAGCCCATC 
TTCGACGGGA TGACACTCTC CCATCCGGAC GATAGCACCA AGAACAAGGA GCTGGACGAT 
TACTACGCTC GCACTAGCAA GAAGTTCATA CTGCTCAATA GAGAGGAGGT CGAGTGCAAC 
CGAGCGAGAG ATGGAAAATC CAATCCGTCC ATGTGCTTCA CCTTCAAGAT AGACGACGCA 
GATGCCGAGG GCTTCGACGT GAGCACCGCC GTCCTGTGGC TCTTCAAGAA CAAACAGAAC 
CGCACCGACA CGGCATCGGT GAACAGCACC AGTGCCCAGC AGACGATCGT AGTCTCCGAG 
GTGGAGGTGG ATCAGCAGAA GGACTCCAAG TATTTGTCGG CGGCCAAGAC CATAGCCATC 
CAGTCGGTGA ATGTGCAAGA TGAGTGGATG AAGATCGACA TTGAGTGGCC CATCAAACAC 
TGGATCAGTG GCCACGAGCT GAGCCACCTC ATCCAAATCA CCTGCGGTGG CTGCGATGTC 
AGCGACATGG AGGAGATCAT TTCGGTGGAC AAGGATTACC GGCCATTCAT CGTGATCGAC 
ATGCAGAACA GGCGGCGCAA GAGTCGCCAG AAGCGCAGCA TCAACTGCTC CAGCGGAATG 
ACCGAGTGCT GTCGGGAGCA CCTGTACATA TCATTCCGGG ACATCGGCTG GAGCAACTGG 
ATCCTGAAGC CGGAGGGCTA CAATGCCTAC TTCTGTCGCG GATCCTGTAG CTCGGTGGCG 
AGTGTTACGC AAGCGGCCTC CCACCACAGT TCCATAATGA AAATTCTCTC CACGAGCGGT 
GCTAACAAGT CGCTGGAGTT GGTGCCCTGC TGCACCGCCA AACAGTATTC CTCGCTGCAG 
CTGGTCGTGA TGGACTCGAG CAACACGGCG ACGGTGAAGA CGCTGCCCAA CATGGTGGTG 
GAGTCATGTG GCTGCAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

daw ( NM_001273038.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259967.1 Drosophila melanogaster dawdle (daw), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa13547
Clone ID Related Accession (Same CDS sequence)	NM_164503.2 , NM_001273038.1 , NM_078737.3
Accession Version	NM_001273038.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dawdle, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCAAGT ACTTTATAGC CCTGGTGTTG CTGGTGTGTC TGGCCCTGGA GAACAGGAAC 
GTATACAATC GCCTGCACGC CCGCTCCCAT CCGAGTTCCC GTGGCGACAT CCTTCGTCCG 
CATCCTAAGC ACCAGCCGCA TCCCCATGTG CAGCACACCT CGCAACAGCT GCAGCAGCAA 
CACCATATCC GGCAGCAACG ATCCCGTCAG CTGAAACACC TGGAACCGGA TCCGACCAGC 
GAGGAGGACG ATGTACCGAT CACCAAGCGG GAGTACTATG CCCGCCTGAG GCGCCACGTC 
ATCCACAAGA GGCAGCACCT CCTGCAGCAG GAGCTCAGCT ACAACCATCC CGGCTCCCAT 
TCGGAGCAGC TAAAGGTGCC CCGCCTGTGG CAACACCTCA TGGAGCAGGA GGAGAAGGCA 
CACCGACATG TGAGCCCGCC CGTGGAGTTG CCTATCATGT ACGGAGATGC CCCCGAGGAG 
TCATCCCTCT CCTCCGATGA TCACTTTGAT GATCTGTCGC CGCTGGACTT TGTGCGCAAC 
GAACTGATGG CCGAGGACCA GCAGAATCAG GAGAAGGATC TCGATCTCGA TCTGGACCTA 
GATCAGCAGC CGGAAACGCC CATAGAGCCA GAGCTCCCAT TGGGTGAGAG GAACATCACC 
ATTTCGGTGA AGTCCAGCGC CGGAGGATGC CCCAAGTGCG AGAGCAATCG CCAAGTGGAG 
CACATCACCG AGGAGCAGCT GACCCACTTA CGCATCGAGT TCGTTAAGCA GCAGATCCTG 
GAGAAGCTGC GCCTCAAGGA GAGCCCCAAG GTGTCGGCCG TGGAGCTGCC CAAGCCCATC 
TTCGACGGGA TGACACTCTC CCATCCGGAC GATAGCACCA AGAACAAGGA GCTGGACGAT 
TACTACGCTC GCACTAGCAA GAAGTTCATA CTGCTCAATA GAGAGGAGGT CGAGTGCAAC 
CGAGCGAGAG ATGGAAAATC CAATCCGTCC ATGTGCTTCA CCTTCAAGAT AGACGACGCA 
GATGCCGAGG GCTTCGACGT GAGCACCGCC GTCCTGTGGC TCTTCAAGAA CAAACAGAAC 
CGCACCGACA CGGCATCGGT GAACAGCACC AGTGCCCAGC AGACGATCGT AGTCTCCGAG 
GTGGAGGTGG ATCAGCAGAA GGACTCCAAG TATTTGTCGG CGGCCAAGAC CATAGCCATC 
CAGTCGGTGA ATGTGCAAGA TGAGTGGATG AAGATCGACA TTGAGTGGCC CATCAAACAC 
TGGATCAGTG GCCACGAGCT GAGCCACCTC ATCCAAATCA CCTGCGGTGG CTGCGATGTC 
AGCGACATGG AGGAGATCAT TTCGGTGGAC AAGGATTACC GGCCATTCAT CGTGATCGAC 
ATGCAGAACA GGCGGCGCAA GAGTCGCCAG AAGCGCAGCA TCAACTGCTC CAGCGGAATG 
ACCGAGTGCT GTCGGGAGCA CCTGTACATA TCATTCCGGG ACATCGGCTG GAGCAACTGG 
ATCCTGAAGC CGGAGGGCTA CAATGCCTAC TTCTGTCGCG GATCCTGTAG CTCGGTGGCG 
AGTGTTACGC AAGCGGCCTC CCACCACAGT TCCATAATGA AAATTCTCTC CACGAGCGGT 
GCTAACAAGT CGCTGGAGTT GGTGCCCTGC TGCACCGCCA AACAGTATTC CTCGCTGCAG 
CTGGTCGTGA TGGACTCGAG CAACACGGCG ACGGTGAAGA CGCTGCCCAA CATGGTGGTG 
GAGTCATGTG GCTGCAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259967.1
CDS	673..2433
Translation	MAKYFIALVLLVCLALENRNVYNRLHARSHPSSRGDILRPHPKHQPHPHVQHTSQQLQQQHHIRQQRSRQLKHLEPDPTSEEDDVPITKREYYARLRRHVIHKRQHLLQQELSYNHPGSHSEQLKVPRLWQHLMEQEEKAHRHVSPPVELPIMYGDAPEESSLSSDDHFDDLSPLDFVRNELMAEDQQNQEKDLDLDLDLDQQPETPIEPELPLGERNITISVKSSAGGCPKCESNRQVEHITEEQLTHLRIEFVKQQILEKLRLKESPKVSAVELPKPIFDGMTLSHPDDSTKNKELDDYYARTSKKFILLNREEVECNRARDGKSNPSMCFTFKIDDADAEGFDVSTAVLWLFKNKQNRTDTASVNSTSAQQTIVVSEVEVDQQKDSKYLSAAKTIAIQSVNVQDEWMKIDIEWPIKHWISGHELSHLIQITCGGCDVSDMEEIISVDKDYRPFIVIDMQNRRRKSRQKRSINCSSGMTECCREHLYISFRDIGWSNWILKPEGYNAYFCRGSCSSVASVTQAASHHSSIMKILSTSGANKSLELVPCCTAKQYSSLQLVVMDSSNTATVKTLPNMVVESCGCR

Target ORF information:

RefSeq Version	NM_001273038.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dawdle (daw), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273038.1

ORF Insert Sequence:

ATGGCCAAGT ACTTTATAGC CCTGGTGTTG CTGGTGTGTC TGGCCCTGGA GAACAGGAAC 
GTATACAATC GCCTGCACGC CCGCTCCCAT CCGAGTTCCC GTGGCGACAT CCTTCGTCCG 
CATCCTAAGC ACCAGCCGCA TCCCCATGTG CAGCACACCT CGCAACAGCT GCAGCAGCAA 
CACCATATCC GGCAGCAACG ATCCCGTCAG CTGAAACACC TGGAACCGGA TCCGACCAGC 
GAGGAGGACG ATGTACCGAT CACCAAGCGG GAGTACTATG CCCGCCTGAG GCGCCACGTC 
ATCCACAAGA GGCAGCACCT CCTGCAGCAG GAGCTCAGCT ACAACCATCC CGGCTCCCAT 
TCGGAGCAGC TAAAGGTGCC CCGCCTGTGG CAACACCTCA TGGAGCAGGA GGAGAAGGCA 
CACCGACATG TGAGCCCGCC CGTGGAGTTG CCTATCATGT ACGGAGATGC CCCCGAGGAG 
TCATCCCTCT CCTCCGATGA TCACTTTGAT GATCTGTCGC CGCTGGACTT TGTGCGCAAC 
GAACTGATGG CCGAGGACCA GCAGAATCAG GAGAAGGATC TCGATCTCGA TCTGGACCTA 
GATCAGCAGC CGGAAACGCC CATAGAGCCA GAGCTCCCAT TGGGTGAGAG GAACATCACC 
ATTTCGGTGA AGTCCAGCGC CGGAGGATGC CCCAAGTGCG AGAGCAATCG CCAAGTGGAG 
CACATCACCG AGGAGCAGCT GACCCACTTA CGCATCGAGT TCGTTAAGCA GCAGATCCTG 
GAGAAGCTGC GCCTCAAGGA GAGCCCCAAG GTGTCGGCCG TGGAGCTGCC CAAGCCCATC 
TTCGACGGGA TGACACTCTC CCATCCGGAC GATAGCACCA AGAACAAGGA GCTGGACGAT 
TACTACGCTC GCACTAGCAA GAAGTTCATA CTGCTCAATA GAGAGGAGGT CGAGTGCAAC 
CGAGCGAGAG ATGGAAAATC CAATCCGTCC ATGTGCTTCA CCTTCAAGAT AGACGACGCA 
GATGCCGAGG GCTTCGACGT GAGCACCGCC GTCCTGTGGC TCTTCAAGAA CAAACAGAAC 
CGCACCGACA CGGCATCGGT GAACAGCACC AGTGCCCAGC AGACGATCGT AGTCTCCGAG 
GTGGAGGTGG ATCAGCAGAA GGACTCCAAG TATTTGTCGG CGGCCAAGAC CATAGCCATC 
CAGTCGGTGA ATGTGCAAGA TGAGTGGATG AAGATCGACA TTGAGTGGCC CATCAAACAC 
TGGATCAGTG GCCACGAGCT GAGCCACCTC ATCCAAATCA CCTGCGGTGG CTGCGATGTC 
AGCGACATGG AGGAGATCAT TTCGGTGGAC AAGGATTACC GGCCATTCAT CGTGATCGAC 
ATGCAGAACA GGCGGCGCAA GAGTCGCCAG AAGCGCAGCA TCAACTGCTC CAGCGGAATG 
ACCGAGTGCT GTCGGGAGCA CCTGTACATA TCATTCCGGG ACATCGGCTG GAGCAACTGG 
ATCCTGAAGC CGGAGGGCTA CAATGCCTAC TTCTGTCGCG GATCCTGTAG CTCGGTGGCG 
AGTGTTACGC AAGCGGCCTC CCACCACAGT TCCATAATGA AAATTCTCTC CACGAGCGGT 
GCTAACAAGT CGCTGGAGTT GGTGCCCTGC TGCACCGCCA AACAGTATTC CTCGCTGCAG 
CTGGTCGTGA TGGACTCGAG CAACACGGCG ACGGTGAAGA CGCTGCCCAA CATGGTGGTG 
GAGTCATGTG GCTGCAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

daw ( NM_078737.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523461.1 Drosophila melanogaster dawdle (daw), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa13547
Clone ID Related Accession (Same CDS sequence)	NM_164503.2 , NM_001273038.1 , NM_078737.3
Accession Version	NM_078737.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	dawdle, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_078737.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCAAGT ACTTTATAGC CCTGGTGTTG CTGGTGTGTC TGGCCCTGGA GAACAGGAAC 
GTATACAATC GCCTGCACGC CCGCTCCCAT CCGAGTTCCC GTGGCGACAT CCTTCGTCCG 
CATCCTAAGC ACCAGCCGCA TCCCCATGTG CAGCACACCT CGCAACAGCT GCAGCAGCAA 
CACCATATCC GGCAGCAACG ATCCCGTCAG CTGAAACACC TGGAACCGGA TCCGACCAGC 
GAGGAGGACG ATGTACCGAT CACCAAGCGG GAGTACTATG CCCGCCTGAG GCGCCACGTC 
ATCCACAAGA GGCAGCACCT CCTGCAGCAG GAGCTCAGCT ACAACCATCC CGGCTCCCAT 
TCGGAGCAGC TAAAGGTGCC CCGCCTGTGG CAACACCTCA TGGAGCAGGA GGAGAAGGCA 
CACCGACATG TGAGCCCGCC CGTGGAGTTG CCTATCATGT ACGGAGATGC CCCCGAGGAG 
TCATCCCTCT CCTCCGATGA TCACTTTGAT GATCTGTCGC CGCTGGACTT TGTGCGCAAC 
GAACTGATGG CCGAGGACCA GCAGAATCAG GAGAAGGATC TCGATCTCGA TCTGGACCTA 
GATCAGCAGC CGGAAACGCC CATAGAGCCA GAGCTCCCAT TGGGTGAGAG GAACATCACC 
ATTTCGGTGA AGTCCAGCGC CGGAGGATGC CCCAAGTGCG AGAGCAATCG CCAAGTGGAG 
CACATCACCG AGGAGCAGCT GACCCACTTA CGCATCGAGT TCGTTAAGCA GCAGATCCTG 
GAGAAGCTGC GCCTCAAGGA GAGCCCCAAG GTGTCGGCCG TGGAGCTGCC CAAGCCCATC 
TTCGACGGGA TGACACTCTC CCATCCGGAC GATAGCACCA AGAACAAGGA GCTGGACGAT 
TACTACGCTC GCACTAGCAA GAAGTTCATA CTGCTCAATA GAGAGGAGGT CGAGTGCAAC 
CGAGCGAGAG ATGGAAAATC CAATCCGTCC ATGTGCTTCA CCTTCAAGAT AGACGACGCA 
GATGCCGAGG GCTTCGACGT GAGCACCGCC GTCCTGTGGC TCTTCAAGAA CAAACAGAAC 
CGCACCGACA CGGCATCGGT GAACAGCACC AGTGCCCAGC AGACGATCGT AGTCTCCGAG 
GTGGAGGTGG ATCAGCAGAA GGACTCCAAG TATTTGTCGG CGGCCAAGAC CATAGCCATC 
CAGTCGGTGA ATGTGCAAGA TGAGTGGATG AAGATCGACA TTGAGTGGCC CATCAAACAC 
TGGATCAGTG GCCACGAGCT GAGCCACCTC ATCCAAATCA CCTGCGGTGG CTGCGATGTC 
AGCGACATGG AGGAGATCAT TTCGGTGGAC AAGGATTACC GGCCATTCAT CGTGATCGAC 
ATGCAGAACA GGCGGCGCAA GAGTCGCCAG AAGCGCAGCA TCAACTGCTC CAGCGGAATG 
ACCGAGTGCT GTCGGGAGCA CCTGTACATA TCATTCCGGG ACATCGGCTG GAGCAACTGG 
ATCCTGAAGC CGGAGGGCTA CAATGCCTAC TTCTGTCGCG GATCCTGTAG CTCGGTGGCG 
AGTGTTACGC AAGCGGCCTC CCACCACAGT TCCATAATGA AAATTCTCTC CACGAGCGGT 
GCTAACAAGT CGCTGGAGTT GGTGCCCTGC TGCACCGCCA AACAGTATTC CTCGCTGCAG 
CTGGTCGTGA TGGACTCGAG CAACACGGCG ACGGTGAAGA CGCTGCCCAA CATGGTGGTG 
GAGTCATGTG GCTGCAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523461.1
CDS	547..2307
Translation	MAKYFIALVLLVCLALENRNVYNRLHARSHPSSRGDILRPHPKHQPHPHVQHTSQQLQQQHHIRQQRSRQLKHLEPDPTSEEDDVPITKREYYARLRRHVIHKRQHLLQQELSYNHPGSHSEQLKVPRLWQHLMEQEEKAHRHVSPPVELPIMYGDAPEESSLSSDDHFDDLSPLDFVRNELMAEDQQNQEKDLDLDLDLDQQPETPIEPELPLGERNITISVKSSAGGCPKCESNRQVEHITEEQLTHLRIEFVKQQILEKLRLKESPKVSAVELPKPIFDGMTLSHPDDSTKNKELDDYYARTSKKFILLNREEVECNRARDGKSNPSMCFTFKIDDADAEGFDVSTAVLWLFKNKQNRTDTASVNSTSAQQTIVVSEVEVDQQKDSKYLSAAKTIAIQSVNVQDEWMKIDIEWPIKHWISGHELSHLIQITCGGCDVSDMEEIISVDKDYRPFIVIDMQNRRRKSRQKRSINCSSGMTECCREHLYISFRDIGWSNWILKPEGYNAYFCRGSCSSVASVTQAASHHSSIMKILSTSGANKSLELVPCCTAKQYSSLQLVVMDSSNTATVKTLPNMVVESCGCR

Target ORF information:

RefSeq Version	NM_078737.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster dawdle (daw), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078737.3

ORF Insert Sequence:

ATGGCCAAGT ACTTTATAGC CCTGGTGTTG CTGGTGTGTC TGGCCCTGGA GAACAGGAAC 
GTATACAATC GCCTGCACGC CCGCTCCCAT CCGAGTTCCC GTGGCGACAT CCTTCGTCCG 
CATCCTAAGC ACCAGCCGCA TCCCCATGTG CAGCACACCT CGCAACAGCT GCAGCAGCAA 
CACCATATCC GGCAGCAACG ATCCCGTCAG CTGAAACACC TGGAACCGGA TCCGACCAGC 
GAGGAGGACG ATGTACCGAT CACCAAGCGG GAGTACTATG CCCGCCTGAG GCGCCACGTC 
ATCCACAAGA GGCAGCACCT CCTGCAGCAG GAGCTCAGCT ACAACCATCC CGGCTCCCAT 
TCGGAGCAGC TAAAGGTGCC CCGCCTGTGG CAACACCTCA TGGAGCAGGA GGAGAAGGCA 
CACCGACATG TGAGCCCGCC CGTGGAGTTG CCTATCATGT ACGGAGATGC CCCCGAGGAG 
TCATCCCTCT CCTCCGATGA TCACTTTGAT GATCTGTCGC CGCTGGACTT TGTGCGCAAC 
GAACTGATGG CCGAGGACCA GCAGAATCAG GAGAAGGATC TCGATCTCGA TCTGGACCTA 
GATCAGCAGC CGGAAACGCC CATAGAGCCA GAGCTCCCAT TGGGTGAGAG GAACATCACC 
ATTTCGGTGA AGTCCAGCGC CGGAGGATGC CCCAAGTGCG AGAGCAATCG CCAAGTGGAG 
CACATCACCG AGGAGCAGCT GACCCACTTA CGCATCGAGT TCGTTAAGCA GCAGATCCTG 
GAGAAGCTGC GCCTCAAGGA GAGCCCCAAG GTGTCGGCCG TGGAGCTGCC CAAGCCCATC 
TTCGACGGGA TGACACTCTC CCATCCGGAC GATAGCACCA AGAACAAGGA GCTGGACGAT 
TACTACGCTC GCACTAGCAA GAAGTTCATA CTGCTCAATA GAGAGGAGGT CGAGTGCAAC 
CGAGCGAGAG ATGGAAAATC CAATCCGTCC ATGTGCTTCA CCTTCAAGAT AGACGACGCA 
GATGCCGAGG GCTTCGACGT GAGCACCGCC GTCCTGTGGC TCTTCAAGAA CAAACAGAAC 
CGCACCGACA CGGCATCGGT GAACAGCACC AGTGCCCAGC AGACGATCGT AGTCTCCGAG 
GTGGAGGTGG ATCAGCAGAA GGACTCCAAG TATTTGTCGG CGGCCAAGAC CATAGCCATC 
CAGTCGGTGA ATGTGCAAGA TGAGTGGATG AAGATCGACA TTGAGTGGCC CATCAAACAC 
TGGATCAGTG GCCACGAGCT GAGCCACCTC ATCCAAATCA CCTGCGGTGG CTGCGATGTC 
AGCGACATGG AGGAGATCAT TTCGGTGGAC AAGGATTACC GGCCATTCAT CGTGATCGAC 
ATGCAGAACA GGCGGCGCAA GAGTCGCCAG AAGCGCAGCA TCAACTGCTC CAGCGGAATG 
ACCGAGTGCT GTCGGGAGCA CCTGTACATA TCATTCCGGG ACATCGGCTG GAGCAACTGG 
ATCCTGAAGC CGGAGGGCTA CAATGCCTAC TTCTGTCGCG GATCCTGTAG CTCGGTGGCG 
AGTGTTACGC AAGCGGCCTC CCACCACAGT TCCATAATGA AAATTCTCTC CACGAGCGGT 
GCTAACAAGT CGCTGGAGTT GGTGCCCTGC TGCACCGCCA AACAGTATTC CTCGCTGCAG 
CTGGTCGTGA TGGACTCGAG CAACACGGCG ACGGTGAAGA CGCTGCCCAA CATGGTGGTG 
GAGTCATGTG GCTGCAGATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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daw cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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