Pkg21D cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pkg21D
Entrez Gene ID	33253
Full Name	Protein kinase, cGMP-dependent at 21D
Synonyms	CG3324,DG1,Dg1,Dmel\CG3324,PKG,PKG 21D,PKG-21D,Pkg1,cGMP,dg1,pkg21D
General protein information	Preferred Names Protein kinase, cGMP-dependent at 21D Names CG3324 gene product from transcript CG3324-RA CG3324-PA Pkg21D-PA cGMP-dependent protein kinase 21D protein kinase, cGMP-dependent at 21D
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 21E2-21E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_057865.4	NP_477213.1	protein kinase, cGMP-dependent at 21D

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	prkg2	NP_001098749.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008585	XP_314690.4
Gallus gallus (chicken)	PRKG2	XP_004941152.1
Drosophila melanogaster (fruit fly)	Pkg21D	NP_477213.1
	PRKG2	XP_001084948.2
Bos taurus (cattle)	PRKG2	NP_001137571.1
Xenopus tropicalis (tropical clawed frog)	prkg2	XP_002934043.2
Homo sapiens (human)	PRKG2	NP_006250.1
Canis lupus familiaris (dog)	PRKG2	XP_003640145.1
Rattus norvegicus (Norway rat)	Prkg2	NP_037144.1
Caenorhabditis elegans (roundworm)	pkg-1	NP_500141.1
Pan troglodytes (chimpanzee)	PRKG2	XP_517194.3
Mus musculus (house mouse)	Prkg2	NP_032952.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DG1 expression greatly enhanced fluid transport by the tubule in response to exogenous cGMP
Title: Analysis of Drosophila cGMP-dependent protein kinases and assessment of their in vivo roles by targeted expression in a renal transporting epithelium.

The following Pkg21D gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pkg21D cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa13387	NM_057865.4 Latest version!	Drosophila melanogaster protein kinase, cGMP-dependent at 21D (Pkg21D), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pkg21D ( NM_057865.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477213.1 Drosophila melanogaster protein kinase, cGMP-dependent at 21D (Pkg21D), mRNA.

Price & Availability↑

CloneID	OFa13387
Clone ID Related Accession (Same CDS sequence)	NM_057865.4
Accession Version	NM_057865.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	protein kinase, cGMP-dependent at 21D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_057865.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGCTG GAATGTTGAC TGATCGGGAG CGGGAGGCGA TCGTCAGCAA TCTGACCAAG 
GACGTGCAGG CTCTGAGGGA AATGGTGCGG AGTCGGGAGA GCGAGCTGGT CAAGCTGCAC 
CGCGAAATCC ACAAGCTGAA GAGTGTTCTC CAGCAGACCA CCAACAACCT GAACGTCACC 
CGAAATGAGA AGGCCAAAAA GAAGCTCTAC TCCTTGCCAG AGCAATGTGG CGAGCAGGAA 
AGTAGGAATC AGAATCCACA TTTGTGCAGC TCCTGCGGCA TGGTGCTGCC CACCAGTCCG 
GAGTTTGCCC TGGAGGCGCT TTCTCTGGGA CCGCTGTCCC CATTAGCATC AACATCTTCC 
GCCTCCCCCT CTGGACGAAC TTCTGCTGAT GAAGTGCGAC CCAAGGCGAT GCCCGCTGCT 
ATCAAGAAAC AAGGTGTCTC CGCCGAGAGC TGTGTGCAGT CCATGCAGCA ATCCTACAGC 
ATACCGATTC CAAAATACGA AAAGGATTTC AGTGATAAGC AGCAAATCAA AGACGCCATT 
ATGGACAATG ACTTCCTAAA GAACATAGAC GCATCGCAGG TGCGGGAACT GGTAGATTCA 
ATGTACTCCA AGAGCATAGC AGCCGGCGAG TTTGTGATCC GCGAGGGTGA AGTGGGCGCA 
CATCTGTACG TCTCCGCCGC CGGCGAGTTT GCGGTGATGC AGCAGGGCAA GGTGCTGGAC 
AAGATGGGGG CGGGAAAGGC GTTTGGGGAG CTGGCTATCC TCTACAACTG CACCAGAACG 
GCCTCCATTC GAGTCCTAAG TGAGGCCGCG AGGGTATGGG TGCTGGACAG ACGGGTCTTT 
CAGCAGATTA TGATGTGCAC GGGCCTCCAG CGAATCGAAA ACAGCGTGAA CTTCCTGAGA 
TCCGTGCCTC TGCTGATGAA CCTTAGTGAG GAGCTGCTGG CCAAGATAGC AGACGTTCTG 
GAGCTGGAGT TCTACGCCGC TGGCACCTAC ATCATCCGCC AAGGAACCGC CGGGGACAGT 
TTCTTCTTGA TCTCACAGGG CAATGTCCGA GTGACCCAAA AACTAACTCC CACCTCCCCG 
GAGGAAACGG AGTTGCGAAC GCTATCCAGG GGAGACTATT TTGGAGAGCA GGCGCTGATC 
AACGAGGATA AGCGAACGGC CAACATCATC GCACTGTCAC CAGGAGTTGA GTGCTTGACC 
CTGGACAGGG ATTCCTTCAA GCGGCTAATT GGGGATCTAT GCGAGCTGAA GGAGAAGGAC 
TACGGCGATG AGAGCAGGAA GCTAGCCATG AAGCAGGCGC AAGAAAGCTG CCGGGATGAG 
CCGAAGGAGC AGCTGCAGCA GGAGTTCCCC GATCTCAAGC TCACGGACCT CGAGGTGGTC 
AGCACTCTTG GCATCGGAGG CTTTGGTCGG GTGGAGCTGG TCAAGGCCCA CCATCAGGAT 
CGCGTGGATA TCTTTGCCCT AAAGTGCCTC AAGAAGCGAC ATATTGTGGA CACCAAACAG 
GAGGAGCACA TCTTCAGTGA ACGTCATATC ATGCTCAGTT CGAGATCGCC CTTCATCTGC 
CGTTTGTATC GCACCTTCCG TGACGAGAAG TACGTGTATA TGCTGCTCGA GGCCTGCATG 
GGTGGGGAGA TATGGACGAT GCTCCGGGAT CGCGGATCCT TCGAGGACAA CGCGGCGCAG 
TTCATCATCG GGTGTGTGCT GCAGGCGTTT GAATACTTGC ATGCCCGCGG GATCATCTAT 
AGAGACCTTA AGCCGGAGAA CCTGATGCTT GATGAGCGCG GCTACGTCAA GATCGTGGAC 
TTCGGCTTTG CCAAGCAGAT CGGAACGAGC TCTAAGACGT GGACCTTCTG CGGCACCCCG 
GAGTACGTGG CACCCGAGAT CATCCTGAAC AAGGGTCACG ATCGAGCCGT AGACTACTGG 
GCTTTGGGCA TTCTCATTCA TGAACTGCTC AACGGAACGC CACCGTTCAG TGCTCCAGAT 
CCCATGCAGA CGTACAATCT CATCCTGAAG GGCATTGACA TGATAGCCTT TCCCAAGCAC 
ATCTCCCGCT GGGCCGTGCA GCTCATCAAG CGACTGTGCC GCGATGTTCC ATCGGAGCGC 
CTCGGCTACC AGACTGGTGG CATACAGGAC ATAAAGAAAC ATAAGTGGTT CCTGGGATTC 
GACTGGGACG GCTTGGCCAG CCAGCTGCTG ATTCCGCCCT TCGTGAGACC CATCGCCCAT 
CCGACGGATG TTCGCTACTT CGACCGCTTT CCGTGCGATT TGAACGAGCC ACCGGACGAG 
CTCTCCGGCT GGGATGCGGA TTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477213.1
CDS	170..2476
Translation	MAAGMLTDREREAIVSNLTKDVQALREMVRSRESELVKLHREIHKLKSVLQQTTNNLNVTRNEKAKKKLYSLPEQCGEQESRNQNPHLCSSCGMVLPTSPEFALEALSLGPLSPLASTSSASPSGRTSADEVRPKAMPAAIKKQGVSAESCVQSMQQSYSIPIPKYEKDFSDKQQIKDAIMDNDFLKNIDASQVRELVDSMYSKSIAAGEFVIREGEVGAHLYVSAAGEFAVMQQGKVLDKMGAGKAFGELAILYNCTRTASIRVLSEAARVWVLDRRVFQQIMMCTGLQRIENSVNFLRSVPLLMNLSEELLAKIADVLELEFYAAGTYIIRQGTAGDSFFLISQGNVRVTQKLTPTSPEETELRTLSRGDYFGEQALINEDKRTANIIALSPGVECLTLDRDSFKRLIGDLCELKEKDYGDESRKLAMKQAQESCRDEPKEQLQQEFPDLKLTDLEVVSTLGIGGFGRVELVKAHHQDRVDIFALKCLKKRHIVDTKQEEHIFSERHIMLSSRSPFICRLYRTFRDEKYVYMLLEACMGGEIWTMLRDRGSFEDNAAQFIIGCVLQAFEYLHARGIIYRDLKPENLMLDERGYVKIVDFGFAKQIGTSSKTWTFCGTPEYVAPEIILNKGHDRAVDYWALGILIHELLNGTPPFSAPDPMQTYNLILKGIDMIAFPKHISRWAVQLIKRLCRDVPSERLGYQTGGIQDIKKHKWFLGFDWDGLASQLLIPPFVRPIAHPTDVRYFDRFPCDLNEPPDELSGWDADF

Target ORF information:

RefSeq Version	NM_057865.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster protein kinase, cGMP-dependent at 21D (Pkg21D), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057865.4

ORF Insert Sequence:

ATGGCCGCTG GAATGTTGAC TGATCGGGAG CGGGAGGCGA TCGTCAGCAA TCTGACCAAG 
GACGTGCAGG CTCTGAGGGA AATGGTGCGG AGTCGGGAGA GCGAGCTGGT CAAGCTGCAC 
CGCGAAATCC ACAAGCTGAA GAGTGTTCTC CAGCAGACCA CCAACAACCT GAACGTCACC 
CGAAATGAGA AGGCCAAAAA GAAGCTCTAC TCCTTGCCAG AGCAATGTGG CGAGCAGGAA 
AGTAGGAATC AGAATCCACA TTTGTGCAGC TCCTGCGGCA TGGTGCTGCC CACCAGTCCG 
GAGTTTGCCC TGGAGGCGCT TTCTCTGGGA CCGCTGTCCC CATTAGCATC AACATCTTCC 
GCCTCCCCCT CTGGACGAAC TTCTGCTGAT GAAGTGCGAC CCAAGGCGAT GCCCGCTGCT 
ATCAAGAAAC AAGGTGTCTC CGCCGAGAGC TGTGTGCAGT CCATGCAGCA ATCCTACAGC 
ATACCGATTC CAAAATACGA AAAGGATTTC AGTGATAAGC AGCAAATCAA AGACGCCATT 
ATGGACAATG ACTTCCTAAA GAACATAGAC GCATCGCAGG TGCGGGAACT GGTAGATTCA 
ATGTACTCCA AGAGCATAGC AGCCGGCGAG TTTGTGATCC GCGAGGGTGA AGTGGGCGCA 
CATCTGTACG TCTCCGCCGC CGGCGAGTTT GCGGTGATGC AGCAGGGCAA GGTGCTGGAC 
AAGATGGGGG CGGGAAAGGC GTTTGGGGAG CTGGCTATCC TCTACAACTG CACCAGAACG 
GCCTCCATTC GAGTCCTAAG TGAGGCCGCG AGGGTATGGG TGCTGGACAG ACGGGTCTTT 
CAGCAGATTA TGATGTGCAC GGGCCTCCAG CGAATCGAAA ACAGCGTGAA CTTCCTGAGA 
TCCGTGCCTC TGCTGATGAA CCTTAGTGAG GAGCTGCTGG CCAAGATAGC AGACGTTCTG 
GAGCTGGAGT TCTACGCCGC TGGCACCTAC ATCATCCGCC AAGGAACCGC CGGGGACAGT 
TTCTTCTTGA TCTCACAGGG CAATGTCCGA GTGACCCAAA AACTAACTCC CACCTCCCCG 
GAGGAAACGG AGTTGCGAAC GCTATCCAGG GGAGACTATT TTGGAGAGCA GGCGCTGATC 
AACGAGGATA AGCGAACGGC CAACATCATC GCACTGTCAC CAGGAGTTGA GTGCTTGACC 
CTGGACAGGG ATTCCTTCAA GCGGCTAATT GGGGATCTAT GCGAGCTGAA GGAGAAGGAC 
TACGGCGATG AGAGCAGGAA GCTAGCCATG AAGCAGGCGC AAGAAAGCTG CCGGGATGAG 
CCGAAGGAGC AGCTGCAGCA GGAGTTCCCC GATCTCAAGC TCACGGACCT CGAGGTGGTC 
AGCACTCTTG GCATCGGAGG CTTTGGTCGG GTGGAGCTGG TCAAGGCCCA CCATCAGGAT 
CGCGTGGATA TCTTTGCCCT AAAGTGCCTC AAGAAGCGAC ATATTGTGGA CACCAAACAG 
GAGGAGCACA TCTTCAGTGA ACGTCATATC ATGCTCAGTT CGAGATCGCC CTTCATCTGC 
CGTTTGTATC GCACCTTCCG TGACGAGAAG TACGTGTATA TGCTGCTCGA GGCCTGCATG 
GGTGGGGAGA TATGGACGAT GCTCCGGGAT CGCGGATCCT TCGAGGACAA CGCGGCGCAG 
TTCATCATCG GGTGTGTGCT GCAGGCGTTT GAATACTTGC ATGCCCGCGG GATCATCTAT 
AGAGACCTTA AGCCGGAGAA CCTGATGCTT GATGAGCGCG GCTACGTCAA GATCGTGGAC 
TTCGGCTTTG CCAAGCAGAT CGGAACGAGC TCTAAGACGT GGACCTTCTG CGGCACCCCG 
GAGTACGTGG CACCCGAGAT CATCCTGAAC AAGGGTCACG ATCGAGCCGT AGACTACTGG 
GCTTTGGGCA TTCTCATTCA TGAACTGCTC AACGGAACGC CACCGTTCAG TGCTCCAGAT 
CCCATGCAGA CGTACAATCT CATCCTGAAG GGCATTGACA TGATAGCCTT TCCCAAGCAC 
ATCTCCCGCT GGGCCGTGCA GCTCATCAAG CGACTGTGCC GCGATGTTCC ATCGGAGCGC 
CTCGGCTACC AGACTGGTGG CATACAGGAC ATAAAGAAAC ATAAGTGGTT CCTGGGATTC 
GACTGGGACG GCTTGGCCAG CCAGCTGCTG ATTCCGCCCT TCGTGAGACC CATCGCCCAT 
CCGACGGATG TTCGCTACTT CGACCGCTTT CCGTGCGATT TGAACGAGCC ACCGGACGAG 
CTCTCCGGCT GGGATGCGGA TTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Pkg21D cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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