Prkg2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prkg2
Entrez Gene ID	25523
Full Name	protein kinase, cGMP-dependent, type II
Synonyms	GDPKII,cGKII
General protein information	Preferred Names protein kinase, cGMP-dependent, type II Names cGMP-dependent protein kinase 2 CGK 2 cGK2 cGMP dependent protein kinase type II cGMP-dependent protein kinase II protein kinase, cGMP- dependent, type 2 type II cGMP-dependent protein kinase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14p22
Summary	may regulate renin release and renal ion transport [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_013012.1	NP_037144.1	cGMP-dependent protein kinase 2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	prkg2	NP_001098749.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008585	XP_314690.4
Gallus gallus (chicken)	PRKG2	XP_004941152.1
Drosophila melanogaster (fruit fly)	Pkg21D	NP_477213.1
	PRKG2	XP_001084948.2
Bos taurus (cattle)	PRKG2	NP_001137571.1
Xenopus tropicalis (tropical clawed frog)	prkg2	XP_002934043.2
Homo sapiens (human)	PRKG2	NP_006250.1
Canis lupus familiaris (dog)	PRKG2	XP_003640145.1
Rattus norvegicus (Norway rat)	Prkg2	NP_037144.1
Caenorhabditis elegans (roundworm)	pkg-1	NP_500141.1
Pan troglodytes (chimpanzee)	PRKG2	XP_517194.3
Mus musculus (house mouse)	Prkg2	NP_032952.3

Gene Ontology
Bibliography

Related articles in PubMed

Type II cGMP-dependent protein kinase negatively regulates fibroblast growth factor signaling by phosphorylating Raf-1 at serine 43 in rat chondrosarcoma cells.
Kamemura N, Murakami S, Komatsu H, Sawanoi M, Miyamoto K, Ishidoh K, Kishimoto K, Tsuji A, Yuasa K
Biochemical and biophysical research communications483(1)82-87(2017 01)

The regulation of synaptic vesicle recycling by cGMP-dependent protein kinase type II in cerebellar granule cells under strong and sustained stimulation.
Collado-Alsina A, Ram?rez-Franco J, S?nchez-Prieto J, Torres M
The Journal of neuroscience : the official journal of the Society for Neuroscience34(26)8788-99(2014 Jun)

The type II cGMP dependent protein kinase regulates GluA1 levels at the plasma membrane of developing cerebellar granule cells.
Incontro S, Ciruela F, Ziff E, Hofmann F, S?nchez-Prieto J, Torres M
Biochimica et biophysica acta1833(8)1820-31(2013 Aug)

Sodium depletion enhances renal expression of (pro)renin receptor via cyclic GMP-protein kinase G signaling pathway.
Huang J, Siragy HM
Hypertension (Dallas, Tex. : 1979)59(2)317-23(2012 Feb)

Phenotypic characterization of the Komeda miniature rat Ishikawa, an animal model of dwarfism caused by a mutation in Prkg2.
Tsuchida A, Yokoi N, Namae M, Fuse M, Masuyama T, Sasaki M, Kawazu S, Komeda K
Comparative medicine58(6)560-7(2008 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Wild-type PKGII phosphorylated Raf-1 at Ser-43 in a cGMP-dependent manner, but a PKGII D412A/R415A mutant, which has a low affinity for cGMP, did not.
Title: Type II cGMP-dependent protein kinase negatively regulates fibroblast growth factor signaling by phosphorylating Raf-1 at serine 43 in rat chondrosarcoma cells.

We conclude that cGKII controls the homeostatic balance of vesicle exocytosis and endocytosis in synaptic boutons of rat cerebellar granule cells.
Title: The regulation of synaptic vesicle recycling by cGMP-dependent protein kinase type II in cerebellar granule cells under strong and sustained stimulation.

Which in turn activates cGKII and induces the phosphorylation of GluA1.
Title: The type II cGMP dependent protein kinase regulates GluA1 levels at the plasma membrane of developing cerebellar granule cells.

Sodium depletion enhances renal expression of (pro)renin receptor via cyclic GMP-protein kinase G signaling pathway.
Title: Sodium depletion enhances renal expression of (pro)renin receptor via cyclic GMP-protein kinase G signaling pathway.

confirmed mutation in Prkg2 is a valuable model for studying dwarfism and longitudinal growth traits
Title: Phenotypic characterization of the Komeda miniature rat Ishikawa, an animal model of dwarfism caused by a mutation in Prkg2.

The following Prkg2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prkg2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00051	NM_013012.1 Latest version!	Rattus norvegicus protein kinase cGMP-dependent 2 (Prkg2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$447.30 $639.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prkg2 ( NM_013012.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037144.1 Rattus norvegicus protein kinase cGMP-dependent 2 (Prkg2), mRNA.

Price & Availability↑

CloneID	ORa00051
Clone ID Related Accession (Same CDS sequence)	NM_013012.1
Accession Version	NM_013012.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Rattus norvegicus(Norway rat)
Product	cGMP-dependent protein kinase 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from Z36276.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Z36276.1 [ECO:0000332] ##Evidence-Data-END##

ATGGGAAATG GTTCAGTGAA ACCCAAGCAT TCGAAGCACC CAGACGGCCA GTCAGGGAAC 
CTCAGCAATG AAGCCCTGCG GAGCAAAGTG GCAGAGCTGG AGCGCGAGGT GAAGAGGAAG 
GATGCAGAAC TTCAGGAGCG CGAGTACCAC CTGAAGGAGC TTCGGGAGCA GCTAGCTAAG 
CAGACCGTGG CCATTGCGGA GCTCACCGAG GAGCTGCAGA GCAAGTGCAT CCAGCTGAAC 
AAGCTGCAGG ATGTTATCCA CGTGCAGGGA GGAAGCCCAC TGCAGGCCTC TCCCGACAAA 
GTGCCTCTGG ATGTTCACCG CAAGACCTCA GGCTTGGTCT CCCTCCACAG CAGGAGGGGG 
GCGAAGGCTG GAGTGTCTGC AGAGCCCACA AGCCGGACCT ATGACCTCAA CAAGCCCCCT 
GAGTTCTCCT TTGAGAAAGC GAGGGTCAGG AAGGATTCCA GTGAGAAAAA ACTTATCACA 
GACGCCCTGA ATAAGAACCA GTTTCTGAAG AGACTGGATC CGCAGCAGAT CAAAGACATG 
GTGGAATGCA TGTACGGGAG AAACTATCAA CAGGGCAGTT ACATCGTTAA GCAAGGGGAA 
CCTGGAAACC ACATCTTTGT GCTGGCCGAG GGTAGACTGG AGGTGTTCCA AGGAGAGAAA 
TTGCTGTCGT CCATCCCAAT GTGGACCACG TTTGGGGAGC TGGCTATTTT GTATAATTGT 
ACGCGGACTG CCTCTGTGAA AGCTATTACC AATGTTAAAA CTTGGGCTCT CGATAGAGAG 
GTTTTCCAGA ACATTATGAG GAGAACAGCG CAAGCCAGGG ATGAGGAATA CAGAAACTTC 
CTCAGAAGTG TCTCCTTGTT GAAGAATTTA CCTGAAGACA AACTAACCAA GATTATTGAC 
TGCTTGGAAG TGGAATACTA TGACAAAGGA GATTATATCA TCAGAGAAGG AGAGGAAGGG 
AGCACCTTTT TCATCTTGGC AAAAGGAAAG GTGAAAGTCA CACAGAGCAC AGAGGGCCAT 
GATCAGCCAC AGTTGATCAA AACACTGCAG AAAGGAGAGT ACTTTGGAGA AAAGGCTCTG 
ATCAGTGATG ATGTCAGATC GGCCAATATA ATCGCGGAAG AAAATGATGT CGCATGCCTC 
GTGATCGACC GAGAAACATT CAACCAAACT GTCGGGACAT TTGATGAACT CCAAAAATAC 
CTTGAGGGGT ATGTGGCCAC CCTGAACCGT GACGATGAAA AGAGACACGC AAAGCGCTCC 
ATGTCTAGCT GGAAGTTGTC CAAAGCCCTT TCTCTGGAGA TGATTCAGCT GAAAGAGAAG 
GTGGCCAGAT TCTCCTCAAC CTCCCCATTC CAGAACCTTG AGATTATCGC AACACTGGGC 
GTTGGTGGGT TCGGAAGAGT GGAGCTTGTT AAAGTTAAAA ATGAGAACAT CGCTTTTGCC 
ATGAAGTGTA TAAGGAAGAA GCACATTGTT GACACTAAAC AGCAGGAACA TGTCTACTCC 
GAGAAGAGGA TCCTGGAGGA GCTGTGTTCT CCCTTCATCG TGAAATTGTA TCGTACTTTC 
AAGGACAATA AATACGTATA CATGCTTCTG GAGGCCTGCT TAGGTGGGGA GCTCTGGAGT 
ATATTAAGGG ACAGAGGCAG CTTCGATGAA CCCACCTCCA AATTCTGTGT GGCCTGCGTG 
ACAGAAGCAT TCGATTATCT ACACCGACTA GGCATTATCT ACAGAGACCT GAAGCCAGAG 
AACTTAATTC TGGATGCCGA TGGCTACCTT AAGTTGGTTG ACTTTGGATT CGCTAAGAAG 
ATTGGCTCTG GGCAGAAAAC GTGGACATTC TGTGGGACTC CAGAGTATGT GGCCCCCGAG 
GTCATTCTTA ACAAAGGACA TGACTTCAGT GTGGATTTCT GGTCCCTGGG GATTCTGGTC 
TATGAGCTTC TAACGGGCAA CCCTCCCTTT TCTGGGATAG ACCAAATGAT GACCTACAAT 
TTGATTCTCA AAGGGATCGA GAAAATGGAT TTTCCTAGAA AGATAACAAG GCGACCCGAG 
GATTTGATCC GGAGGCTTTG CAGGCAAAAC CCAACAGAAA GGCTGGGGAA CCTGAAGAAT 
GGGATCAACG ACATTAAGAA ACACAGGTGG TTAAACGGTT TCAATTGGGA GGGGTTGAAA 
GCACGGAGCC TTCCCTCACC TTTACGAAGA GAGCTCAGTG GCCCCATAGA TCACAGCTAC 
TTTGACAAGT ATCCTCCTGA AAAGGGGGTT CCTCCGGATG AAATGTCAGG CTGGGATAAG 
GACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037144.1
CDS	48..2336
Translation	MGNGSVKPKHSKHPDGQSGNLSNEALRSKVAELEREVKRKDAELQEREYHLKELREQLAKQTVAIAELTEELQSKCIQLNKLQDVIHVQGGSPLQASPDKVPLDVHRKTSGLVSLHSRRGAKAGVSAEPTSRTYDLNKPPEFSFEKARVRKDSSEKKLITDALNKNQFLKRLDPQQIKDMVECMYGRNYQQGSYIVKQGEPGNHIFVLAEGRLEVFQGEKLLSSIPMWTTFGELAILYNCTRTASVKAITNVKTWALDREVFQNIMRRTAQARDEEYRNFLRSVSLLKNLPEDKLTKIIDCLEVEYYDKGDYIIREGEEGSTFFILAKGKVKVTQSTEGHDQPQLIKTLQKGEYFGEKALISDDVRSANIIAEENDVACLVIDRETFNQTVGTFDELQKYLEGYVATLNRDDEKRHAKRSMSSWKLSKALSLEMIQLKEKVARFSSTSPFQNLEIIATLGVGGFGRVELVKVKNENIAFAMKCIRKKHIVDTKQQEHVYSEKRILEELCSPFIVKLYRTFKDNKYVYMLLEACLGGELWSILRDRGSFDEPTSKFCVACVTEAFDYLHRLGIIYRDLKPENLILDADGYLKLVDFGFAKKIGSGQKTWTFCGTPEYVAPEVILNKGHDFSVDFWSLGILVYELLTGNPPFSGIDQMMTYNLILKGIEKMDFPRKITRRPEDLIRRLCRQNPTERLGNLKNGINDIKKHRWLNGFNWEGLKARSLPSPLRRELSGPIDHSYFDKYPPEKGVPPDEMSGWDKDF

Target ORF information:

RefSeq Version	NM_013012.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein kinase cGMP-dependent 2 (Prkg2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013012.1

ORF Insert Sequence:

ATGGGAAATG GTTCAGTGAA ACCCAAGCAT TCGAAGCACC CAGACGGCCA GTCAGGGAAC 
CTCAGCAATG AAGCCCTGCG GAGCAAAGTG GCAGAGCTGG AGCGCGAGGT GAAGAGGAAG 
GATGCAGAAC TTCAGGAGCG CGAGTACCAC CTGAAGGAGC TTCGGGAGCA GCTAGCTAAG 
CAGACCGTGG CCATTGCGGA GCTCACCGAG GAGCTGCAGA GCAAGTGCAT CCAGCTGAAC 
AAGCTGCAGG ATGTTATCCA CGTGCAGGGA GGAAGCCCAC TGCAGGCCTC TCCCGACAAA 
GTGCCTCTGG ATGTTCACCG CAAGACCTCA GGCTTGGTCT CCCTCCACAG CAGGAGGGGG 
GCGAAGGCTG GAGTGTCTGC AGAGCCCACA AGCCGGACCT ATGACCTCAA CAAGCCCCCT 
GAGTTCTCCT TTGAGAAAGC GAGGGTCAGG AAGGATTCCA GTGAGAAAAA ACTTATCACA 
GACGCCCTGA ATAAGAACCA GTTTCTGAAG AGACTGGATC CGCAGCAGAT CAAAGACATG 
GTGGAATGCA TGTACGGGAG AAACTATCAA CAGGGCAGTT ACATCGTTAA GCAAGGGGAA 
CCTGGAAACC ACATCTTTGT GCTGGCCGAG GGTAGACTGG AGGTGTTCCA AGGAGAGAAA 
TTGCTGTCGT CCATCCCAAT GTGGACCACG TTTGGGGAGC TGGCTATTTT GTATAATTGT 
ACGCGGACTG CCTCTGTGAA AGCTATTACC AATGTTAAAA CTTGGGCTCT CGATAGAGAG 
GTTTTCCAGA ACATTATGAG GAGAACAGCG CAAGCCAGGG ATGAGGAATA CAGAAACTTC 
CTCAGAAGTG TCTCCTTGTT GAAGAATTTA CCTGAAGACA AACTAACCAA GATTATTGAC 
TGCTTGGAAG TGGAATACTA TGACAAAGGA GATTATATCA TCAGAGAAGG AGAGGAAGGG 
AGCACCTTTT TCATCTTGGC AAAAGGAAAG GTGAAAGTCA CACAGAGCAC AGAGGGCCAT 
GATCAGCCAC AGTTGATCAA AACACTGCAG AAAGGAGAGT ACTTTGGAGA AAAGGCTCTG 
ATCAGTGATG ATGTCAGATC GGCCAATATA ATCGCGGAAG AAAATGATGT CGCATGCCTC 
GTGATCGACC GAGAAACATT CAACCAAACT GTCGGGACAT TTGATGAACT CCAAAAATAC 
CTTGAGGGGT ATGTGGCCAC CCTGAACCGT GACGATGAAA AGAGACACGC AAAGCGCTCC 
ATGTCTAGCT GGAAGTTGTC CAAAGCCCTT TCTCTGGAGA TGATTCAGCT GAAAGAGAAG 
GTGGCCAGAT TCTCCTCAAC CTCCCCATTC CAGAACCTTG AGATTATCGC AACACTGGGC 
GTTGGTGGGT TCGGAAGAGT GGAGCTTGTT AAAGTTAAAA ATGAGAACAT CGCTTTTGCC 
ATGAAGTGTA TAAGGAAGAA GCACATTGTT GACACTAAAC AGCAGGAACA TGTCTACTCC 
GAGAAGAGGA TCCTGGAGGA GCTGTGTTCT CCCTTCATCG TGAAATTGTA TCGTACTTTC 
AAGGACAATA AATACGTATA CATGCTTCTG GAGGCCTGCT TAGGTGGGGA GCTCTGGAGT 
ATATTAAGGG ACAGAGGCAG CTTCGATGAA CCCACCTCCA AATTCTGTGT GGCCTGCGTG 
ACAGAAGCAT TCGATTATCT ACACCGACTA GGCATTATCT ACAGAGACCT GAAGCCAGAG 
AACTTAATTC TGGATGCCGA TGGCTACCTT AAGTTGGTTG ACTTTGGATT CGCTAAGAAG 
ATTGGCTCTG GGCAGAAAAC GTGGACATTC TGTGGGACTC CAGAGTATGT GGCCCCCGAG 
GTCATTCTTA ACAAAGGACA TGACTTCAGT GTGGATTTCT GGTCCCTGGG GATTCTGGTC 
TATGAGCTTC TAACGGGCAA CCCTCCCTTT TCTGGGATAG ACCAAATGAT GACCTACAAT 
TTGATTCTCA AAGGGATCGA GAAAATGGAT TTTCCTAGAA AGATAACAAG GCGACCCGAG 
GATTTGATCC GGAGGCTTTG CAGGCAAAAC CCAACAGAAA GGCTGGGGAA CCTGAAGAAT 
GGGATCAACG ACATTAAGAA ACACAGGTGG TTAAACGGTT TCAATTGGGA GGGGTTGAAA 
GCACGGAGCC TTCCCTCACC TTTACGAAGA GAGCTCAGTG GCCCCATAGA TCACAGCTAC 
TTTGACAAGT ATCCTCCTGA AAAGGGGGTT CCTCCGGATG AAATGTCAGG CTGGGATAAG 
GACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Type II cGMP-dependent protein kinase negatively regulates fibroblast growth factor signaling by phosphorylating Raf-1 at serine 43 in rat chondrosarcoma cells.
Biochemical and biophysical research communications483(1)82-87(2017 01)
Kamemura N,Murakami S,Komatsu H,Sawanoi M,Miyamoto K,Ishidoh K,Kishimoto K,Tsuji A,Yuasa K

The regulation of synaptic vesicle recycling by cGMP-dependent protein kinase type II in cerebellar granule cells under strong and sustained stimulation.
The Journal of neuroscience : the official journal of the Society for Neuroscience34(26)8788-99(2014 Jun)
Collado-Alsina A,Ram?rez-Franco J,S?nchez-Prieto J,Torres M

The type II cGMP dependent protein kinase regulates GluA1 levels at the plasma membrane of developing cerebellar granule cells.
Biochimica et biophysica acta1833(8)1820-31(2013 Aug)
Incontro S,Ciruela F,Ziff E,Hofmann F,S?nchez-Prieto J,Torres M

Sodium depletion enhances renal expression of (pro)renin receptor via cyclic GMP-protein kinase G signaling pathway.
Hypertension (Dallas, Tex. : 1979)59(2)317-23(2012 Feb)
Huang J,Siragy HM

Phenotypic characterization of the Komeda miniature rat Ishikawa, an animal model of dwarfism caused by a mutation in Prkg2.
Comparative medicine58(6)560-7(2008 Dec)
Tsuchida A,Yokoi N,Namae M,Fuse M,Masuyama T,Sasaki M,Kawazu S,Komeda K

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Prkg2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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