PRKG2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRKG2
Entrez Gene ID	533330
Full Name	protein kinase, cGMP-dependent, type II
General protein information	Preferred Names protein kinase, cGMP-dependent, type II Names cGMP-dependent protein kinase 2 cGMP-dependant type II protein kinase
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024993286.1	XP_024849054.1	cGMP-dependent protein kinase 2 isoform X1
XM_024993288.1	XP_024849056.1	cGMP-dependent protein kinase 2 isoform X1
NM_001144099.1	NP_001137571.1	cGMP-dependent protein kinase 2
XM_024993287.1	XP_024849055.1	cGMP-dependent protein kinase 2 isoform X1
XM_024993289.1	XP_024849057.1	cGMP-dependent protein kinase 2 isoform X2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	prkg2	NP_001098749.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008585	XP_314690.4
Gallus gallus (chicken)	PRKG2	XP_004941152.1
Drosophila melanogaster (fruit fly)	Pkg21D	NP_477213.1
	PRKG2	XP_001084948.2
Bos taurus (cattle)	PRKG2	NP_001137571.1
Xenopus tropicalis (tropical clawed frog)	prkg2	XP_002934043.2
Homo sapiens (human)	PRKG2	NP_006250.1
Canis lupus familiaris (dog)	PRKG2	XP_003640145.1
Rattus norvegicus (Norway rat)	Prkg2	NP_037144.1
Caenorhabditis elegans (roundworm)	pkg-1	NP_500141.1
Pan troglodytes (chimpanzee)	PRKG2	XP_517194.3
Mus musculus (house mouse)	Prkg2	NP_032952.3

Gene Ontology
Bibliography

Related articles in PubMed

A nonsense mutation in cGMP-dependent type II protein kinase (PRKG2) causes dwarfism in American Angus cattle.
Koltes JE, Mishra BP, Kumar D, Kataria RS, Totir LR, Fernando RL, Cobbold R, Steffen D, Coppieters W, Georges M, Reecy JM
Proceedings of the National Academy of Sciences of the United States of America106(46)19250-5(2009 Nov)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study indicates that loss of PRKG2 function results in differential expression of P53 regulated genes as well as additional pathways consistent with increased proliferation and apoptosis in the growth plate due to achondroplastic dwarfism.
Title: Transcriptional profiling of PRKG2-null growth plate identifies putative down-stream targets of PRKG2.

PRKG2 R678X as a causative mutation for dwarfism cattle was presented.
Title: A nonsense mutation in cGMP-dependent type II protein kinase (PRKG2) causes dwarfism in American Angus cattle.

The following PRKG2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRKG2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa235370	XM_024993289.1 Latest version!	Bos taurus protein kinase cGMP-dependent 2 (PRKG2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OBa36618	NM_001144099.1 Latest version!	Bos taurus protein kinase, cGMP-dependent, type II (PRKG2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa36618	XM_024993286.1 Latest version!	Bos taurus protein kinase cGMP-dependent 2 (PRKG2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa36618	XM_024993287.1 Latest version!	Bos taurus protein kinase cGMP-dependent 2 (PRKG2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa36618	XM_024993288.1 Latest version!	Bos taurus protein kinase cGMP-dependent 2 (PRKG2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRKG2 ( XM_024993289.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849057.1 Bos taurus protein kinase cGMP-dependent 2 (PRKG2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa235370
Clone ID Related Accession (Same CDS sequence)	XM_024993289.1
Accession Version	XM_024993289.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 849bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	cGMP-dependent protein kinase 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTGTA TAAGGAAGAA GCACATCGTT GACACGAAGC AGCAGGAGCA TGTCTACTCA 
GAAAAGAAGA TCCTGGAAGA GCTATGCTCA CCCTTCATTG TGAAATTATA TCGCACCTTC 
AAGGACAATA AGTATGTATA CATGCTTCTG GAGGCCTGCT TAGGTGGGGA GCTGTGGAGT 
ATATTAAGAG ACAGAGGCAG CTTTGATGAA CCCACCTCCA AGTTCTGTGT TGCCTGTGTG 
ACAGAAGCAT TCGATTACCT GCATCGACTA GGTATTATCT ACAGAGACCT GAAACCAGAA 
AACTTAATTC TAGATGCTGA GGGCTATCTT AAATTGGTTG ACTTTGGATT TGCTAAGAAA 
ATAGGATCTG GACAGAAAAC ATGGACGTTC TGTGGAACTC CAGAGTATGT AGCTCCTGAA 
GTCATTCTCA ACAAAGGACA TGACTTCAGT GTGGATTTTT GGTCCCTGGG AATTCTAGTA 
TATGAGCTCC TCACGGGCAA CCCTCCTTTT TCTGGGATTG ACCAAATGAT GACCTACAAT 
CTGATTCTCA AAGGCATTGA AAAAATGGAT TTTCCCAGAA AGATAACAAG ACGACCTGAG 
GATCTGATTC GGAGGCTTTG CAGGCAAAAT CCAACAGAAA GACTGGGAAA TCTGAAGAAT 
GGAATCAATG ACATTAAGAA ACACAGGTGG TTAAATGGTT TTAATTGGGA GGGACTGAAA 
GCACGAAATC TTCCATCACC TTTACAAAGA GAGCTCAGTG GACCCACAGA TCACAGCTAC 
TTTGACAAGT ATCCTCCTGA AAGGGGGGTG CCTCCAGATG AGCTGTCAGG CTGGGACAAA 
GACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849057.1
CDS	159..1007
Translation	MKCIRKKHIVDTKQQEHVYSEKKILEELCSPFIVKLYRTFKDNKYVYMLLEACLGGELWSILRDRGSFDEPTSKFCVACVTEAFDYLHRLGIIYRDLKPENLILDAEGYLKLVDFGFAKKIGSGQKTWTFCGTPEYVAPEVILNKGHDFSVDFWSLGILVYELLTGNPPFSGIDQMMTYNLILKGIEKMDFPRKITRRPEDLIRRLCRQNPTERLGNLKNGINDIKKHRWLNGFNWEGLKARNLPSPLQRELSGPTDHSYFDKYPPERGVPPDELSGWDKDF

Target ORF information:

RefSeq Version	XM_024993289.1
Organism	Bos taurus(cattle)
Definition	Bos taurus protein kinase cGMP-dependent 2 (PRKG2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993289.1

ORF Insert Sequence:

ATGAAGTGTA TAAGGAAGAA GCACATCGTT GACACGAAGC AGCAGGAGCA TGTCTACTCA 
GAAAAGAAGA TCCTGGAAGA GCTATGCTCA CCCTTCATTG TGAAATTATA TCGCACCTTC 
AAGGACAATA AGTATGTATA CATGCTTCTG GAGGCCTGCT TAGGTGGGGA GCTGTGGAGT 
ATATTAAGAG ACAGAGGCAG CTTTGATGAA CCCACCTCCA AGTTCTGTGT TGCCTGTGTG 
ACAGAAGCAT TCGATTACCT GCATCGACTA GGTATTATCT ACAGAGACCT GAAACCAGAA 
AACTTAATTC TAGATGCTGA GGGCTATCTT AAATTGGTTG ACTTTGGATT TGCTAAGAAA 
ATAGGATCTG GACAGAAAAC ATGGACGTTC TGTGGAACTC CAGAGTATGT AGCTCCTGAA 
GTCATTCTCA ACAAAGGACA TGACTTCAGT GTGGATTTTT GGTCCCTGGG AATTCTAGTA 
TATGAGCTCC TCACGGGCAA CCCTCCTTTT TCTGGGATTG ACCAAATGAT GACCTACAAT 
CTGATTCTCA AAGGCATTGA AAAAATGGAT TTTCCCAGAA AGATAACAAG ACGACCTGAG 
GATCTGATTC GGAGGCTTTG CAGGCAAAAT CCAACAGAAA GACTGGGAAA TCTGAAGAAT 
GGAATCAATG ACATTAAGAA ACACAGGTGG TTAAATGGTT TTAATTGGGA GGGACTGAAA 
GCACGAAATC TTCCATCACC TTTACAAAGA GAGCTCAGTG GACCCACAGA TCACAGCTAC 
TTTGACAAGT ATCCTCCTGA AAGGGGGGTG CCTCCAGATG AGCTGTCAGG CTGGGACAAA 
GACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKG2 ( NM_001144099.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001137571.1 Bos taurus protein kinase, cGMP-dependent, type II (PRKG2), mRNA.

Price & Availability↑

CloneID	OBa36618
Clone ID Related Accession (Same CDS sequence)	XM_024993286.1 , XM_024993288.1 , NM_001144099.1 , XM_024993287.1
Accession Version	NM_001144099.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-03
Organism	Bos taurus(cattle)
Product	cGMP-dependent protein kinase 2
Comment	Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from AAFC03077640.1, AAFC03077643.1, AAFC03077638.1 and AAFC03077642.1. On Jan 22, 2009 this sequence version replaced XM_612707.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01093728, SAMN01093729 [ECO:0000348] ##Evidence-Data-END##

ATGGGAAATG GTTCGGTGAA ACCCAAACAC TCCAAGAATC CAGATGGGCA CCCCGGGAAC 
CTCACCGCCA GCGCCCTCCG GAGCAGGGTG ACAGAGCTGG AAAGAGAGCT GAGAAGGAAG 
GATGCTGAGA TCCAGGAGCG GGAATACCAC CTGAAGGAGC TGCGGGAGCA GCTGTCCAAA 
CAGACTGTGG CCATCGCGGA GCTCACGGAG GAGCTCCAGA ACAAGTGCAT CCAGCTGAAC 
AAGCTTCAGG ATGTGGTGCA CCTGCAGGGA GGAAGCCTGC CCCGGGCGTC CCCGGACAAA 
GTGCCTCTTG AGGTTCAGCG GAAGACCTCG GGATTGGTCT CCCTCCACAG CAGGAGGGGA 
GCGAAGGCTG GAGTGTCCGC CGAGCCGACC ACCCGAACCT ATGACCTCAA CAAACCCCCT 
GAATTTTCCT TTGAGAAAGC AAGAGTCAGA AAGGACTCCA GTGAGAAGAA GCTCATTACA 
GATGCCCTTA ATAAAAATCA ATTTCTGAAG AGACTGGATC CTCAGCAGAT CAAAGACATG 
GTGGAATGCA TGTATGGGAG AAACTACCAG CAAGGGAGCT ACATTATTAA GCAAGGAGAA 
CCAGGAAACC ATATCTTTGT ACTGGCAGAG GGTCGACTAG AGGTGTCCCA AGGGGAGAAA 
TTGCTGTCAT CCATCCCTAT GTGGACCACG TTTGGGGAGC TAGCCATTTT ATACAACTGT 
ACAAGAACTG CCTCTGTGAA AGCTATTACC AATGTTAAAA CATGGGCACT AGATCGAGAG 
GTATTCCAGA ATATAATGAG AAGGACAGCT CAAGCTAGAG ATGAACAGTA CAGAAACTTC 
CTCAGAAGTG TATCCTTGCT GAAGAATTTA CCTGAAGATA AACTAACCAA GATCATTGAC 
TGCTTGGAAG TGGAATACTA TGACAAAGGA GATTACATTA TTAGAGAGGG CGAGGAAGGA 
AGTACGTTTT TCATTTTAGC AAAAGGAAAG GTAAAAGTCA CTCAAAGTAC TGAAGGCCAT 
GATCAACCAC AGCTAATAAA AACACTGCAG AAAGGAGAAT ATTTTGGAGA AAAAGCTCTT 
ATCAGTGAAG ATGTCAGATC AGCTAACATT ATCGCCGAGG AAAATGATGT CGCATGCCTG 
GTTATAGATC GAGAAACATT CAACCAAACA GTGGGGACTT TTGAAGAACT CCAGAAATAT 
CTTGAAGGGT ATGTGGCAAA CCTGAACCGG GATGATGAAA AAAGACATGC GAAGAGATCC 
ATGTCTAGCT GGAAGCTGTC CAAAGCACTC TCTTTGGAGA TGATTCAACT GAAGGAGAAA 
GTGGCCAGAT TTTCCTCATC ATCCCCCTTC CAGAACCTTG AGATTATCGC AACACTGGGC 
GTTGGTGGGT TCGGAAGAGT TGAGCTTGTT AAGGTGAAAA ATGAGAACGT TGCTTTTGCT 
ATGAAGTGTA TAAGGAAGAA GCACATCGTT GACACGAAGC AGCAGGAGCA TGTCTACTCA 
GAAAAGAAGA TCCTGGAAGA GCTATGCTCA CCCTTCATTG TGAAATTATA TCGCACCTTC 
AAGGACAATA AGTATGTATA CATGCTTCTG GAGGCCTGCT TAGGTGGGGA GCTGTGGAGT 
ATATTAAGAG ACAGAGGCAG CTTTGATGAA CCCACCTCCA AGTTCTGTGT TGCCTGTGTG 
ACAGAAGCAT TCGATTACCT GCATCGACTA GGTATTATCT ACAGAGACCT GAAACCAGAA 
AACTTAATTC TAGATGCTGA GGGCTATCTT AAATTGGTTG ACTTTGGATT TGCTAAGAAA 
ATAGGATCTG GACAGAAAAC ATGGACGTTC TGTGGAACTC CAGAGTATGT AGCTCCTGAA 
GTCATTCTCA ACAAAGGACA TGACTTCAGT GTGGATTTTT GGTCCCTGGG AATTCTAGTA 
TATGAGCTCC TCACGGGCAA CCCTCCTTTT TCTGGGATTG ACCAAATGAT GACCTACAAT 
CTGATTCTCA AAGGCATTGA AAAAATGGAT TTTCCCAGAA AGATAACAAG ACGACCTGAG 
GATCTGATTC GGAGGCTTTG CAGGCAAAAT CCAACAGAAA GACTGGGAAA TCTGAAGAAT 
GGAATCAATG ACATTAAGAA ACACAGGTGG TTAAATGGTT TTAATTGGGA GGGACTGAAA 
GCACGAAATC TTCCATCACC TTTACAAAGA GAGCTCAGTG GACCCACAGA TCACAGCTAC 
TTTGACAAGT ATCCTCCTGA AAGGGGGGTG CCTCCAGATG AGCTGTCAGG CTGGGACAAA 
GACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137571.1
CDS	1..2289
Translation	MGNGSVKPKHSKNPDGHPGNLTASALRSRVTELERELRRKDAEIQEREYHLKELREQLSKQTVAIAELTEELQNKCIQLNKLQDVVHLQGGSLPRASPDKVPLEVQRKTSGLVSLHSRRGAKAGVSAEPTTRTYDLNKPPEFSFEKARVRKDSSEKKLITDALNKNQFLKRLDPQQIKDMVECMYGRNYQQGSYIIKQGEPGNHIFVLAEGRLEVSQGEKLLSSIPMWTTFGELAILYNCTRTASVKAITNVKTWALDREVFQNIMRRTAQARDEQYRNFLRSVSLLKNLPEDKLTKIIDCLEVEYYDKGDYIIREGEEGSTFFILAKGKVKVTQSTEGHDQPQLIKTLQKGEYFGEKALISEDVRSANIIAEENDVACLVIDRETFNQTVGTFEELQKYLEGYVANLNRDDEKRHAKRSMSSWKLSKALSLEMIQLKEKVARFSSSSPFQNLEIIATLGVGGFGRVELVKVKNENVAFAMKCIRKKHIVDTKQQEHVYSEKKILEELCSPFIVKLYRTFKDNKYVYMLLEACLGGELWSILRDRGSFDEPTSKFCVACVTEAFDYLHRLGIIYRDLKPENLILDAEGYLKLVDFGFAKKIGSGQKTWTFCGTPEYVAPEVILNKGHDFSVDFWSLGILVYELLTGNPPFSGIDQMMTYNLILKGIEKMDFPRKITRRPEDLIRRLCRQNPTERLGNLKNGINDIKKHRWLNGFNWEGLKARNLPSPLQRELSGPTDHSYFDKYPPERGVPPDELSGWDKDF

Target ORF information:

RefSeq Version	NM_001144099.1
Organism	Bos taurus(cattle)
Definition	Bos taurus protein kinase, cGMP-dependent, type II (PRKG2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144099.1

ORF Insert Sequence:

ATGGGAAATG GTTCGGTGAA ACCCAAACAC TCCAAGAATC CAGATGGGCA CCCCGGGAAC 
CTCACCGCCA GCGCCCTCCG GAGCAGGGTG ACAGAGCTGG AAAGAGAGCT GAGAAGGAAG 
GATGCTGAGA TCCAGGAGCG GGAATACCAC CTGAAGGAGC TGCGGGAGCA GCTGTCCAAA 
CAGACTGTGG CCATCGCGGA GCTCACGGAG GAGCTCCAGA ACAAGTGCAT CCAGCTGAAC 
AAGCTTCAGG ATGTGGTGCA CCTGCAGGGA GGAAGCCTGC CCCGGGCGTC CCCGGACAAA 
GTGCCTCTTG AGGTTCAGCG GAAGACCTCG GGATTGGTCT CCCTCCACAG CAGGAGGGGA 
GCGAAGGCTG GAGTGTCCGC CGAGCCGACC ACCCGAACCT ATGACCTCAA CAAACCCCCT 
GAATTTTCCT TTGAGAAAGC AAGAGTCAGA AAGGACTCCA GTGAGAAGAA GCTCATTACA 
GATGCCCTTA ATAAAAATCA ATTTCTGAAG AGACTGGATC CTCAGCAGAT CAAAGACATG 
GTGGAATGCA TGTATGGGAG AAACTACCAG CAAGGGAGCT ACATTATTAA GCAAGGAGAA 
CCAGGAAACC ATATCTTTGT ACTGGCAGAG GGTCGACTAG AGGTGTCCCA AGGGGAGAAA 
TTGCTGTCAT CCATCCCTAT GTGGACCACG TTTGGGGAGC TAGCCATTTT ATACAACTGT 
ACAAGAACTG CCTCTGTGAA AGCTATTACC AATGTTAAAA CATGGGCACT AGATCGAGAG 
GTATTCCAGA ATATAATGAG AAGGACAGCT CAAGCTAGAG ATGAACAGTA CAGAAACTTC 
CTCAGAAGTG TATCCTTGCT GAAGAATTTA CCTGAAGATA AACTAACCAA GATCATTGAC 
TGCTTGGAAG TGGAATACTA TGACAAAGGA GATTACATTA TTAGAGAGGG CGAGGAAGGA 
AGTACGTTTT TCATTTTAGC AAAAGGAAAG GTAAAAGTCA CTCAAAGTAC TGAAGGCCAT 
GATCAACCAC AGCTAATAAA AACACTGCAG AAAGGAGAAT ATTTTGGAGA AAAAGCTCTT 
ATCAGTGAAG ATGTCAGATC AGCTAACATT ATCGCCGAGG AAAATGATGT CGCATGCCTG 
GTTATAGATC GAGAAACATT CAACCAAACA GTGGGGACTT TTGAAGAACT CCAGAAATAT 
CTTGAAGGGT ATGTGGCAAA CCTGAACCGG GATGATGAAA AAAGACATGC GAAGAGATCC 
ATGTCTAGCT GGAAGCTGTC CAAAGCACTC TCTTTGGAGA TGATTCAACT GAAGGAGAAA 
GTGGCCAGAT TTTCCTCATC ATCCCCCTTC CAGAACCTTG AGATTATCGC AACACTGGGC 
GTTGGTGGGT TCGGAAGAGT TGAGCTTGTT AAGGTGAAAA ATGAGAACGT TGCTTTTGCT 
ATGAAGTGTA TAAGGAAGAA GCACATCGTT GACACGAAGC AGCAGGAGCA TGTCTACTCA 
GAAAAGAAGA TCCTGGAAGA GCTATGCTCA CCCTTCATTG TGAAATTATA TCGCACCTTC 
AAGGACAATA AGTATGTATA CATGCTTCTG GAGGCCTGCT TAGGTGGGGA GCTGTGGAGT 
ATATTAAGAG ACAGAGGCAG CTTTGATGAA CCCACCTCCA AGTTCTGTGT TGCCTGTGTG 
ACAGAAGCAT TCGATTACCT GCATCGACTA GGTATTATCT ACAGAGACCT GAAACCAGAA 
AACTTAATTC TAGATGCTGA GGGCTATCTT AAATTGGTTG ACTTTGGATT TGCTAAGAAA 
ATAGGATCTG GACAGAAAAC ATGGACGTTC TGTGGAACTC CAGAGTATGT AGCTCCTGAA 
GTCATTCTCA ACAAAGGACA TGACTTCAGT GTGGATTTTT GGTCCCTGGG AATTCTAGTA 
TATGAGCTCC TCACGGGCAA CCCTCCTTTT TCTGGGATTG ACCAAATGAT GACCTACAAT 
CTGATTCTCA AAGGCATTGA AAAAATGGAT TTTCCCAGAA AGATAACAAG ACGACCTGAG 
GATCTGATTC GGAGGCTTTG CAGGCAAAAT CCAACAGAAA GACTGGGAAA TCTGAAGAAT 
GGAATCAATG ACATTAAGAA ACACAGGTGG TTAAATGGTT TTAATTGGGA GGGACTGAAA 
GCACGAAATC TTCCATCACC TTTACAAAGA GAGCTCAGTG GACCCACAGA TCACAGCTAC 
TTTGACAAGT ATCCTCCTGA AAGGGGGGTG CCTCCAGATG AGCTGTCAGG CTGGGACAAA 
GACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKG2 ( XM_024993286.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849054.1 Bos taurus protein kinase cGMP-dependent 2 (PRKG2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa36618
Clone ID Related Accession (Same CDS sequence)	XM_024993286.1 , XM_024993288.1 , NM_001144099.1 , XM_024993287.1
Accession Version	XM_024993286.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	cGMP-dependent protein kinase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAATG GTTCGGTGAA ACCCAAACAC TCCAAGAATC CAGATGGGCA CCCCGGGAAC 
CTCACCGCCA GCGCCCTCCG GAGCAGGGTG ACAGAGCTGG AAAGAGAGCT GAGAAGGAAG 
GATGCTGAGA TCCAGGAGCG GGAATACCAC CTGAAGGAGC TGCGGGAGCA GCTGTCCAAA 
CAGACTGTGG CCATCGCGGA GCTCACGGAG GAGCTCCAGA ACAAGTGCAT CCAGCTGAAC 
AAGCTTCAGG ATGTGGTGCA CCTGCAGGGA GGAAGCCTGC CCCGGGCGTC CCCGGACAAA 
GTGCCTCTTG AGGTTCAGCG GAAGACCTCG GGATTGGTCT CCCTCCACAG CAGGAGGGGA 
GCGAAGGCTG GAGTGTCCGC CGAGCCGACC ACCCGAACCT ATGACCTCAA CAAACCCCCT 
GAATTTTCCT TTGAGAAAGC AAGAGTCAGA AAGGACTCCA GTGAGAAGAA GCTCATTACA 
GATGCCCTTA ATAAAAATCA ATTTCTGAAG AGACTGGATC CTCAGCAGAT CAAAGACATG 
GTGGAATGCA TGTATGGGAG AAACTACCAG CAAGGGAGCT ACATTATTAA GCAAGGAGAA 
CCAGGAAACC ATATCTTTGT ACTGGCAGAG GGTCGACTAG AGGTGTCCCA AGGGGAGAAA 
TTGCTGTCAT CCATCCCTAT GTGGACCACG TTTGGGGAGC TAGCCATTTT ATACAACTGT 
ACAAGAACTG CCTCTGTGAA AGCTATTACC AATGTTAAAA CATGGGCACT AGATCGAGAG 
GTATTCCAGA ATATAATGAG AAGGACAGCT CAAGCTAGAG ATGAACAGTA CAGAAACTTC 
CTCAGAAGTG TATCCTTGCT GAAGAATTTA CCTGAAGATA AACTAACCAA GATCATTGAC 
TGCTTGGAAG TGGAATACTA TGACAAAGGA GATTACATTA TTAGAGAGGG CGAGGAAGGA 
AGTACGTTTT TCATTTTAGC AAAAGGAAAG GTAAAAGTCA CTCAAAGTAC TGAAGGCCAT 
GATCAACCAC AGCTAATAAA AACACTGCAG AAAGGAGAAT ATTTTGGAGA AAAAGCTCTT 
ATCAGTGAAG ATGTCAGATC AGCTAACATT ATCGCCGAGG AAAATGATGT CGCATGCCTG 
GTTATAGATC GAGAAACATT CAACCAAACA GTGGGGACTT TTGAAGAACT CCAGAAATAT 
CTTGAAGGGT ATGTGGCAAA CCTGAACCGG GATGATGAAA AAAGACATGC GAAGAGATCC 
ATGTCTAGCT GGAAGCTGTC CAAAGCACTC TCTTTGGAGA TGATTCAACT GAAGGAGAAA 
GTGGCCAGAT TTTCCTCATC ATCCCCCTTC CAGAACCTTG AGATTATCGC AACACTGGGC 
GTTGGTGGGT TCGGAAGAGT TGAGCTTGTT AAGGTGAAAA ATGAGAACGT TGCTTTTGCT 
ATGAAGTGTA TAAGGAAGAA GCACATCGTT GACACGAAGC AGCAGGAGCA TGTCTACTCA 
GAAAAGAAGA TCCTGGAAGA GCTATGCTCA CCCTTCATTG TGAAATTATA TCGCACCTTC 
AAGGACAATA AGTATGTATA CATGCTTCTG GAGGCCTGCT TAGGTGGGGA GCTGTGGAGT 
ATATTAAGAG ACAGAGGCAG CTTTGATGAA CCCACCTCCA AGTTCTGTGT TGCCTGTGTG 
ACAGAAGCAT TCGATTACCT GCATCGACTA GGTATTATCT ACAGAGACCT GAAACCAGAA 
AACTTAATTC TAGATGCTGA GGGCTATCTT AAATTGGTTG ACTTTGGATT TGCTAAGAAA 
ATAGGATCTG GACAGAAAAC ATGGACGTTC TGTGGAACTC CAGAGTATGT AGCTCCTGAA 
GTCATTCTCA ACAAAGGACA TGACTTCAGT GTGGATTTTT GGTCCCTGGG AATTCTAGTA 
TATGAGCTCC TCACGGGCAA CCCTCCTTTT TCTGGGATTG ACCAAATGAT GACCTACAAT 
CTGATTCTCA AAGGCATTGA AAAAATGGAT TTTCCCAGAA AGATAACAAG ACGACCTGAG 
GATCTGATTC GGAGGCTTTG CAGGCAAAAT CCAACAGAAA GACTGGGAAA TCTGAAGAAT 
GGAATCAATG ACATTAAGAA ACACAGGTGG TTAAATGGTT TTAATTGGGA GGGACTGAAA 
GCACGAAATC TTCCATCACC TTTACAAAGA GAGCTCAGTG GACCCACAGA TCACAGCTAC 
TTTGACAAGT ATCCTCCTGA AAGGGGGGTG CCTCCAGATG AGCTGTCAGG CTGGGACAAA 
GACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849054.1
CDS	135..2423
Translation	MGNGSVKPKHSKNPDGHPGNLTASALRSRVTELERELRRKDAEIQEREYHLKELREQLSKQTVAIAELTEELQNKCIQLNKLQDVVHLQGGSLPRASPDKVPLEVQRKTSGLVSLHSRRGAKAGVSAEPTTRTYDLNKPPEFSFEKARVRKDSSEKKLITDALNKNQFLKRLDPQQIKDMVECMYGRNYQQGSYIIKQGEPGNHIFVLAEGRLEVSQGEKLLSSIPMWTTFGELAILYNCTRTASVKAITNVKTWALDREVFQNIMRRTAQARDEQYRNFLRSVSLLKNLPEDKLTKIIDCLEVEYYDKGDYIIREGEEGSTFFILAKGKVKVTQSTEGHDQPQLIKTLQKGEYFGEKALISEDVRSANIIAEENDVACLVIDRETFNQTVGTFEELQKYLEGYVANLNRDDEKRHAKRSMSSWKLSKALSLEMIQLKEKVARFSSSSPFQNLEIIATLGVGGFGRVELVKVKNENVAFAMKCIRKKHIVDTKQQEHVYSEKKILEELCSPFIVKLYRTFKDNKYVYMLLEACLGGELWSILRDRGSFDEPTSKFCVACVTEAFDYLHRLGIIYRDLKPENLILDAEGYLKLVDFGFAKKIGSGQKTWTFCGTPEYVAPEVILNKGHDFSVDFWSLGILVYELLTGNPPFSGIDQMMTYNLILKGIEKMDFPRKITRRPEDLIRRLCRQNPTERLGNLKNGINDIKKHRWLNGFNWEGLKARNLPSPLQRELSGPTDHSYFDKYPPERGVPPDELSGWDKDF

Target ORF information:

RefSeq Version	XM_024993286.1
Organism	Bos taurus(cattle)
Definition	Bos taurus protein kinase cGMP-dependent 2 (PRKG2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993286.1

ORF Insert Sequence:

ATGGGAAATG GTTCGGTGAA ACCCAAACAC TCCAAGAATC CAGATGGGCA CCCCGGGAAC 
CTCACCGCCA GCGCCCTCCG GAGCAGGGTG ACAGAGCTGG AAAGAGAGCT GAGAAGGAAG 
GATGCTGAGA TCCAGGAGCG GGAATACCAC CTGAAGGAGC TGCGGGAGCA GCTGTCCAAA 
CAGACTGTGG CCATCGCGGA GCTCACGGAG GAGCTCCAGA ACAAGTGCAT CCAGCTGAAC 
AAGCTTCAGG ATGTGGTGCA CCTGCAGGGA GGAAGCCTGC CCCGGGCGTC CCCGGACAAA 
GTGCCTCTTG AGGTTCAGCG GAAGACCTCG GGATTGGTCT CCCTCCACAG CAGGAGGGGA 
GCGAAGGCTG GAGTGTCCGC CGAGCCGACC ACCCGAACCT ATGACCTCAA CAAACCCCCT 
GAATTTTCCT TTGAGAAAGC AAGAGTCAGA AAGGACTCCA GTGAGAAGAA GCTCATTACA 
GATGCCCTTA ATAAAAATCA ATTTCTGAAG AGACTGGATC CTCAGCAGAT CAAAGACATG 
GTGGAATGCA TGTATGGGAG AAACTACCAG CAAGGGAGCT ACATTATTAA GCAAGGAGAA 
CCAGGAAACC ATATCTTTGT ACTGGCAGAG GGTCGACTAG AGGTGTCCCA AGGGGAGAAA 
TTGCTGTCAT CCATCCCTAT GTGGACCACG TTTGGGGAGC TAGCCATTTT ATACAACTGT 
ACAAGAACTG CCTCTGTGAA AGCTATTACC AATGTTAAAA CATGGGCACT AGATCGAGAG 
GTATTCCAGA ATATAATGAG AAGGACAGCT CAAGCTAGAG ATGAACAGTA CAGAAACTTC 
CTCAGAAGTG TATCCTTGCT GAAGAATTTA CCTGAAGATA AACTAACCAA GATCATTGAC 
TGCTTGGAAG TGGAATACTA TGACAAAGGA GATTACATTA TTAGAGAGGG CGAGGAAGGA 
AGTACGTTTT TCATTTTAGC AAAAGGAAAG GTAAAAGTCA CTCAAAGTAC TGAAGGCCAT 
GATCAACCAC AGCTAATAAA AACACTGCAG AAAGGAGAAT ATTTTGGAGA AAAAGCTCTT 
ATCAGTGAAG ATGTCAGATC AGCTAACATT ATCGCCGAGG AAAATGATGT CGCATGCCTG 
GTTATAGATC GAGAAACATT CAACCAAACA GTGGGGACTT TTGAAGAACT CCAGAAATAT 
CTTGAAGGGT ATGTGGCAAA CCTGAACCGG GATGATGAAA AAAGACATGC GAAGAGATCC 
ATGTCTAGCT GGAAGCTGTC CAAAGCACTC TCTTTGGAGA TGATTCAACT GAAGGAGAAA 
GTGGCCAGAT TTTCCTCATC ATCCCCCTTC CAGAACCTTG AGATTATCGC AACACTGGGC 
GTTGGTGGGT TCGGAAGAGT TGAGCTTGTT AAGGTGAAAA ATGAGAACGT TGCTTTTGCT 
ATGAAGTGTA TAAGGAAGAA GCACATCGTT GACACGAAGC AGCAGGAGCA TGTCTACTCA 
GAAAAGAAGA TCCTGGAAGA GCTATGCTCA CCCTTCATTG TGAAATTATA TCGCACCTTC 
AAGGACAATA AGTATGTATA CATGCTTCTG GAGGCCTGCT TAGGTGGGGA GCTGTGGAGT 
ATATTAAGAG ACAGAGGCAG CTTTGATGAA CCCACCTCCA AGTTCTGTGT TGCCTGTGTG 
ACAGAAGCAT TCGATTACCT GCATCGACTA GGTATTATCT ACAGAGACCT GAAACCAGAA 
AACTTAATTC TAGATGCTGA GGGCTATCTT AAATTGGTTG ACTTTGGATT TGCTAAGAAA 
ATAGGATCTG GACAGAAAAC ATGGACGTTC TGTGGAACTC CAGAGTATGT AGCTCCTGAA 
GTCATTCTCA ACAAAGGACA TGACTTCAGT GTGGATTTTT GGTCCCTGGG AATTCTAGTA 
TATGAGCTCC TCACGGGCAA CCCTCCTTTT TCTGGGATTG ACCAAATGAT GACCTACAAT 
CTGATTCTCA AAGGCATTGA AAAAATGGAT TTTCCCAGAA AGATAACAAG ACGACCTGAG 
GATCTGATTC GGAGGCTTTG CAGGCAAAAT CCAACAGAAA GACTGGGAAA TCTGAAGAAT 
GGAATCAATG ACATTAAGAA ACACAGGTGG TTAAATGGTT TTAATTGGGA GGGACTGAAA 
GCACGAAATC TTCCATCACC TTTACAAAGA GAGCTCAGTG GACCCACAGA TCACAGCTAC 
TTTGACAAGT ATCCTCCTGA AAGGGGGGTG CCTCCAGATG AGCTGTCAGG CTGGGACAAA 
GACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKG2 ( XM_024993288.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849056.1 Bos taurus protein kinase cGMP-dependent 2 (PRKG2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa36618
Clone ID Related Accession (Same CDS sequence)	XM_024993286.1 , XM_024993288.1 , NM_001144099.1 , XM_024993287.1
Accession Version	XM_024993288.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	cGMP-dependent protein kinase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAATG GTTCGGTGAA ACCCAAACAC TCCAAGAATC CAGATGGGCA CCCCGGGAAC 
CTCACCGCCA GCGCCCTCCG GAGCAGGGTG ACAGAGCTGG AAAGAGAGCT GAGAAGGAAG 
GATGCTGAGA TCCAGGAGCG GGAATACCAC CTGAAGGAGC TGCGGGAGCA GCTGTCCAAA 
CAGACTGTGG CCATCGCGGA GCTCACGGAG GAGCTCCAGA ACAAGTGCAT CCAGCTGAAC 
AAGCTTCAGG ATGTGGTGCA CCTGCAGGGA GGAAGCCTGC CCCGGGCGTC CCCGGACAAA 
GTGCCTCTTG AGGTTCAGCG GAAGACCTCG GGATTGGTCT CCCTCCACAG CAGGAGGGGA 
GCGAAGGCTG GAGTGTCCGC CGAGCCGACC ACCCGAACCT ATGACCTCAA CAAACCCCCT 
GAATTTTCCT TTGAGAAAGC AAGAGTCAGA AAGGACTCCA GTGAGAAGAA GCTCATTACA 
GATGCCCTTA ATAAAAATCA ATTTCTGAAG AGACTGGATC CTCAGCAGAT CAAAGACATG 
GTGGAATGCA TGTATGGGAG AAACTACCAG CAAGGGAGCT ACATTATTAA GCAAGGAGAA 
CCAGGAAACC ATATCTTTGT ACTGGCAGAG GGTCGACTAG AGGTGTCCCA AGGGGAGAAA 
TTGCTGTCAT CCATCCCTAT GTGGACCACG TTTGGGGAGC TAGCCATTTT ATACAACTGT 
ACAAGAACTG CCTCTGTGAA AGCTATTACC AATGTTAAAA CATGGGCACT AGATCGAGAG 
GTATTCCAGA ATATAATGAG AAGGACAGCT CAAGCTAGAG ATGAACAGTA CAGAAACTTC 
CTCAGAAGTG TATCCTTGCT GAAGAATTTA CCTGAAGATA AACTAACCAA GATCATTGAC 
TGCTTGGAAG TGGAATACTA TGACAAAGGA GATTACATTA TTAGAGAGGG CGAGGAAGGA 
AGTACGTTTT TCATTTTAGC AAAAGGAAAG GTAAAAGTCA CTCAAAGTAC TGAAGGCCAT 
GATCAACCAC AGCTAATAAA AACACTGCAG AAAGGAGAAT ATTTTGGAGA AAAAGCTCTT 
ATCAGTGAAG ATGTCAGATC AGCTAACATT ATCGCCGAGG AAAATGATGT CGCATGCCTG 
GTTATAGATC GAGAAACATT CAACCAAACA GTGGGGACTT TTGAAGAACT CCAGAAATAT 
CTTGAAGGGT ATGTGGCAAA CCTGAACCGG GATGATGAAA AAAGACATGC GAAGAGATCC 
ATGTCTAGCT GGAAGCTGTC CAAAGCACTC TCTTTGGAGA TGATTCAACT GAAGGAGAAA 
GTGGCCAGAT TTTCCTCATC ATCCCCCTTC CAGAACCTTG AGATTATCGC AACACTGGGC 
GTTGGTGGGT TCGGAAGAGT TGAGCTTGTT AAGGTGAAAA ATGAGAACGT TGCTTTTGCT 
ATGAAGTGTA TAAGGAAGAA GCACATCGTT GACACGAAGC AGCAGGAGCA TGTCTACTCA 
GAAAAGAAGA TCCTGGAAGA GCTATGCTCA CCCTTCATTG TGAAATTATA TCGCACCTTC 
AAGGACAATA AGTATGTATA CATGCTTCTG GAGGCCTGCT TAGGTGGGGA GCTGTGGAGT 
ATATTAAGAG ACAGAGGCAG CTTTGATGAA CCCACCTCCA AGTTCTGTGT TGCCTGTGTG 
ACAGAAGCAT TCGATTACCT GCATCGACTA GGTATTATCT ACAGAGACCT GAAACCAGAA 
AACTTAATTC TAGATGCTGA GGGCTATCTT AAATTGGTTG ACTTTGGATT TGCTAAGAAA 
ATAGGATCTG GACAGAAAAC ATGGACGTTC TGTGGAACTC CAGAGTATGT AGCTCCTGAA 
GTCATTCTCA ACAAAGGACA TGACTTCAGT GTGGATTTTT GGTCCCTGGG AATTCTAGTA 
TATGAGCTCC TCACGGGCAA CCCTCCTTTT TCTGGGATTG ACCAAATGAT GACCTACAAT 
CTGATTCTCA AAGGCATTGA AAAAATGGAT TTTCCCAGAA AGATAACAAG ACGACCTGAG 
GATCTGATTC GGAGGCTTTG CAGGCAAAAT CCAACAGAAA GACTGGGAAA TCTGAAGAAT 
GGAATCAATG ACATTAAGAA ACACAGGTGG TTAAATGGTT TTAATTGGGA GGGACTGAAA 
GCACGAAATC TTCCATCACC TTTACAAAGA GAGCTCAGTG GACCCACAGA TCACAGCTAC 
TTTGACAAGT ATCCTCCTGA AAGGGGGGTG CCTCCAGATG AGCTGTCAGG CTGGGACAAA 
GACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849056.1
CDS	35..2323
Translation	MGNGSVKPKHSKNPDGHPGNLTASALRSRVTELERELRRKDAEIQEREYHLKELREQLSKQTVAIAELTEELQNKCIQLNKLQDVVHLQGGSLPRASPDKVPLEVQRKTSGLVSLHSRRGAKAGVSAEPTTRTYDLNKPPEFSFEKARVRKDSSEKKLITDALNKNQFLKRLDPQQIKDMVECMYGRNYQQGSYIIKQGEPGNHIFVLAEGRLEVSQGEKLLSSIPMWTTFGELAILYNCTRTASVKAITNVKTWALDREVFQNIMRRTAQARDEQYRNFLRSVSLLKNLPEDKLTKIIDCLEVEYYDKGDYIIREGEEGSTFFILAKGKVKVTQSTEGHDQPQLIKTLQKGEYFGEKALISEDVRSANIIAEENDVACLVIDRETFNQTVGTFEELQKYLEGYVANLNRDDEKRHAKRSMSSWKLSKALSLEMIQLKEKVARFSSSSPFQNLEIIATLGVGGFGRVELVKVKNENVAFAMKCIRKKHIVDTKQQEHVYSEKKILEELCSPFIVKLYRTFKDNKYVYMLLEACLGGELWSILRDRGSFDEPTSKFCVACVTEAFDYLHRLGIIYRDLKPENLILDAEGYLKLVDFGFAKKIGSGQKTWTFCGTPEYVAPEVILNKGHDFSVDFWSLGILVYELLTGNPPFSGIDQMMTYNLILKGIEKMDFPRKITRRPEDLIRRLCRQNPTERLGNLKNGINDIKKHRWLNGFNWEGLKARNLPSPLQRELSGPTDHSYFDKYPPERGVPPDELSGWDKDF

Target ORF information:

RefSeq Version	XM_024993288.1
Organism	Bos taurus(cattle)
Definition	Bos taurus protein kinase cGMP-dependent 2 (PRKG2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993288.1

ORF Insert Sequence:

ATGGGAAATG GTTCGGTGAA ACCCAAACAC TCCAAGAATC CAGATGGGCA CCCCGGGAAC 
CTCACCGCCA GCGCCCTCCG GAGCAGGGTG ACAGAGCTGG AAAGAGAGCT GAGAAGGAAG 
GATGCTGAGA TCCAGGAGCG GGAATACCAC CTGAAGGAGC TGCGGGAGCA GCTGTCCAAA 
CAGACTGTGG CCATCGCGGA GCTCACGGAG GAGCTCCAGA ACAAGTGCAT CCAGCTGAAC 
AAGCTTCAGG ATGTGGTGCA CCTGCAGGGA GGAAGCCTGC CCCGGGCGTC CCCGGACAAA 
GTGCCTCTTG AGGTTCAGCG GAAGACCTCG GGATTGGTCT CCCTCCACAG CAGGAGGGGA 
GCGAAGGCTG GAGTGTCCGC CGAGCCGACC ACCCGAACCT ATGACCTCAA CAAACCCCCT 
GAATTTTCCT TTGAGAAAGC AAGAGTCAGA AAGGACTCCA GTGAGAAGAA GCTCATTACA 
GATGCCCTTA ATAAAAATCA ATTTCTGAAG AGACTGGATC CTCAGCAGAT CAAAGACATG 
GTGGAATGCA TGTATGGGAG AAACTACCAG CAAGGGAGCT ACATTATTAA GCAAGGAGAA 
CCAGGAAACC ATATCTTTGT ACTGGCAGAG GGTCGACTAG AGGTGTCCCA AGGGGAGAAA 
TTGCTGTCAT CCATCCCTAT GTGGACCACG TTTGGGGAGC TAGCCATTTT ATACAACTGT 
ACAAGAACTG CCTCTGTGAA AGCTATTACC AATGTTAAAA CATGGGCACT AGATCGAGAG 
GTATTCCAGA ATATAATGAG AAGGACAGCT CAAGCTAGAG ATGAACAGTA CAGAAACTTC 
CTCAGAAGTG TATCCTTGCT GAAGAATTTA CCTGAAGATA AACTAACCAA GATCATTGAC 
TGCTTGGAAG TGGAATACTA TGACAAAGGA GATTACATTA TTAGAGAGGG CGAGGAAGGA 
AGTACGTTTT TCATTTTAGC AAAAGGAAAG GTAAAAGTCA CTCAAAGTAC TGAAGGCCAT 
GATCAACCAC AGCTAATAAA AACACTGCAG AAAGGAGAAT ATTTTGGAGA AAAAGCTCTT 
ATCAGTGAAG ATGTCAGATC AGCTAACATT ATCGCCGAGG AAAATGATGT CGCATGCCTG 
GTTATAGATC GAGAAACATT CAACCAAACA GTGGGGACTT TTGAAGAACT CCAGAAATAT 
CTTGAAGGGT ATGTGGCAAA CCTGAACCGG GATGATGAAA AAAGACATGC GAAGAGATCC 
ATGTCTAGCT GGAAGCTGTC CAAAGCACTC TCTTTGGAGA TGATTCAACT GAAGGAGAAA 
GTGGCCAGAT TTTCCTCATC ATCCCCCTTC CAGAACCTTG AGATTATCGC AACACTGGGC 
GTTGGTGGGT TCGGAAGAGT TGAGCTTGTT AAGGTGAAAA ATGAGAACGT TGCTTTTGCT 
ATGAAGTGTA TAAGGAAGAA GCACATCGTT GACACGAAGC AGCAGGAGCA TGTCTACTCA 
GAAAAGAAGA TCCTGGAAGA GCTATGCTCA CCCTTCATTG TGAAATTATA TCGCACCTTC 
AAGGACAATA AGTATGTATA CATGCTTCTG GAGGCCTGCT TAGGTGGGGA GCTGTGGAGT 
ATATTAAGAG ACAGAGGCAG CTTTGATGAA CCCACCTCCA AGTTCTGTGT TGCCTGTGTG 
ACAGAAGCAT TCGATTACCT GCATCGACTA GGTATTATCT ACAGAGACCT GAAACCAGAA 
AACTTAATTC TAGATGCTGA GGGCTATCTT AAATTGGTTG ACTTTGGATT TGCTAAGAAA 
ATAGGATCTG GACAGAAAAC ATGGACGTTC TGTGGAACTC CAGAGTATGT AGCTCCTGAA 
GTCATTCTCA ACAAAGGACA TGACTTCAGT GTGGATTTTT GGTCCCTGGG AATTCTAGTA 
TATGAGCTCC TCACGGGCAA CCCTCCTTTT TCTGGGATTG ACCAAATGAT GACCTACAAT 
CTGATTCTCA AAGGCATTGA AAAAATGGAT TTTCCCAGAA AGATAACAAG ACGACCTGAG 
GATCTGATTC GGAGGCTTTG CAGGCAAAAT CCAACAGAAA GACTGGGAAA TCTGAAGAAT 
GGAATCAATG ACATTAAGAA ACACAGGTGG TTAAATGGTT TTAATTGGGA GGGACTGAAA 
GCACGAAATC TTCCATCACC TTTACAAAGA GAGCTCAGTG GACCCACAGA TCACAGCTAC 
TTTGACAAGT ATCCTCCTGA AAGGGGGGTG CCTCCAGATG AGCTGTCAGG CTGGGACAAA 
GACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKG2 ( XM_024993287.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024849055.1 Bos taurus protein kinase cGMP-dependent 2 (PRKG2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa36618
Clone ID Related Accession (Same CDS sequence)	XM_024993286.1 , XM_024993288.1 , NM_001144099.1 , XM_024993287.1
Accession Version	XM_024993287.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	cGMP-dependent protein kinase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAAATG GTTCGGTGAA ACCCAAACAC TCCAAGAATC CAGATGGGCA CCCCGGGAAC 
CTCACCGCCA GCGCCCTCCG GAGCAGGGTG ACAGAGCTGG AAAGAGAGCT GAGAAGGAAG 
GATGCTGAGA TCCAGGAGCG GGAATACCAC CTGAAGGAGC TGCGGGAGCA GCTGTCCAAA 
CAGACTGTGG CCATCGCGGA GCTCACGGAG GAGCTCCAGA ACAAGTGCAT CCAGCTGAAC 
AAGCTTCAGG ATGTGGTGCA CCTGCAGGGA GGAAGCCTGC CCCGGGCGTC CCCGGACAAA 
GTGCCTCTTG AGGTTCAGCG GAAGACCTCG GGATTGGTCT CCCTCCACAG CAGGAGGGGA 
GCGAAGGCTG GAGTGTCCGC CGAGCCGACC ACCCGAACCT ATGACCTCAA CAAACCCCCT 
GAATTTTCCT TTGAGAAAGC AAGAGTCAGA AAGGACTCCA GTGAGAAGAA GCTCATTACA 
GATGCCCTTA ATAAAAATCA ATTTCTGAAG AGACTGGATC CTCAGCAGAT CAAAGACATG 
GTGGAATGCA TGTATGGGAG AAACTACCAG CAAGGGAGCT ACATTATTAA GCAAGGAGAA 
CCAGGAAACC ATATCTTTGT ACTGGCAGAG GGTCGACTAG AGGTGTCCCA AGGGGAGAAA 
TTGCTGTCAT CCATCCCTAT GTGGACCACG TTTGGGGAGC TAGCCATTTT ATACAACTGT 
ACAAGAACTG CCTCTGTGAA AGCTATTACC AATGTTAAAA CATGGGCACT AGATCGAGAG 
GTATTCCAGA ATATAATGAG AAGGACAGCT CAAGCTAGAG ATGAACAGTA CAGAAACTTC 
CTCAGAAGTG TATCCTTGCT GAAGAATTTA CCTGAAGATA AACTAACCAA GATCATTGAC 
TGCTTGGAAG TGGAATACTA TGACAAAGGA GATTACATTA TTAGAGAGGG CGAGGAAGGA 
AGTACGTTTT TCATTTTAGC AAAAGGAAAG GTAAAAGTCA CTCAAAGTAC TGAAGGCCAT 
GATCAACCAC AGCTAATAAA AACACTGCAG AAAGGAGAAT ATTTTGGAGA AAAAGCTCTT 
ATCAGTGAAG ATGTCAGATC AGCTAACATT ATCGCCGAGG AAAATGATGT CGCATGCCTG 
GTTATAGATC GAGAAACATT CAACCAAACA GTGGGGACTT TTGAAGAACT CCAGAAATAT 
CTTGAAGGGT ATGTGGCAAA CCTGAACCGG GATGATGAAA AAAGACATGC GAAGAGATCC 
ATGTCTAGCT GGAAGCTGTC CAAAGCACTC TCTTTGGAGA TGATTCAACT GAAGGAGAAA 
GTGGCCAGAT TTTCCTCATC ATCCCCCTTC CAGAACCTTG AGATTATCGC AACACTGGGC 
GTTGGTGGGT TCGGAAGAGT TGAGCTTGTT AAGGTGAAAA ATGAGAACGT TGCTTTTGCT 
ATGAAGTGTA TAAGGAAGAA GCACATCGTT GACACGAAGC AGCAGGAGCA TGTCTACTCA 
GAAAAGAAGA TCCTGGAAGA GCTATGCTCA CCCTTCATTG TGAAATTATA TCGCACCTTC 
AAGGACAATA AGTATGTATA CATGCTTCTG GAGGCCTGCT TAGGTGGGGA GCTGTGGAGT 
ATATTAAGAG ACAGAGGCAG CTTTGATGAA CCCACCTCCA AGTTCTGTGT TGCCTGTGTG 
ACAGAAGCAT TCGATTACCT GCATCGACTA GGTATTATCT ACAGAGACCT GAAACCAGAA 
AACTTAATTC TAGATGCTGA GGGCTATCTT AAATTGGTTG ACTTTGGATT TGCTAAGAAA 
ATAGGATCTG GACAGAAAAC ATGGACGTTC TGTGGAACTC CAGAGTATGT AGCTCCTGAA 
GTCATTCTCA ACAAAGGACA TGACTTCAGT GTGGATTTTT GGTCCCTGGG AATTCTAGTA 
TATGAGCTCC TCACGGGCAA CCCTCCTTTT TCTGGGATTG ACCAAATGAT GACCTACAAT 
CTGATTCTCA AAGGCATTGA AAAAATGGAT TTTCCCAGAA AGATAACAAG ACGACCTGAG 
GATCTGATTC GGAGGCTTTG CAGGCAAAAT CCAACAGAAA GACTGGGAAA TCTGAAGAAT 
GGAATCAATG ACATTAAGAA ACACAGGTGG TTAAATGGTT TTAATTGGGA GGGACTGAAA 
GCACGAAATC TTCCATCACC TTTACAAAGA GAGCTCAGTG GACCCACAGA TCACAGCTAC 
TTTGACAAGT ATCCTCCTGA AAGGGGGGTG CCTCCAGATG AGCTGTCAGG CTGGGACAAA 
GACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024849055.1
CDS	125..2413
Translation	MGNGSVKPKHSKNPDGHPGNLTASALRSRVTELERELRRKDAEIQEREYHLKELREQLSKQTVAIAELTEELQNKCIQLNKLQDVVHLQGGSLPRASPDKVPLEVQRKTSGLVSLHSRRGAKAGVSAEPTTRTYDLNKPPEFSFEKARVRKDSSEKKLITDALNKNQFLKRLDPQQIKDMVECMYGRNYQQGSYIIKQGEPGNHIFVLAEGRLEVSQGEKLLSSIPMWTTFGELAILYNCTRTASVKAITNVKTWALDREVFQNIMRRTAQARDEQYRNFLRSVSLLKNLPEDKLTKIIDCLEVEYYDKGDYIIREGEEGSTFFILAKGKVKVTQSTEGHDQPQLIKTLQKGEYFGEKALISEDVRSANIIAEENDVACLVIDRETFNQTVGTFEELQKYLEGYVANLNRDDEKRHAKRSMSSWKLSKALSLEMIQLKEKVARFSSSSPFQNLEIIATLGVGGFGRVELVKVKNENVAFAMKCIRKKHIVDTKQQEHVYSEKKILEELCSPFIVKLYRTFKDNKYVYMLLEACLGGELWSILRDRGSFDEPTSKFCVACVTEAFDYLHRLGIIYRDLKPENLILDAEGYLKLVDFGFAKKIGSGQKTWTFCGTPEYVAPEVILNKGHDFSVDFWSLGILVYELLTGNPPFSGIDQMMTYNLILKGIEKMDFPRKITRRPEDLIRRLCRQNPTERLGNLKNGINDIKKHRWLNGFNWEGLKARNLPSPLQRELSGPTDHSYFDKYPPERGVPPDELSGWDKDF

Target ORF information:

RefSeq Version	XM_024993287.1
Organism	Bos taurus(cattle)
Definition	Bos taurus protein kinase cGMP-dependent 2 (PRKG2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024993287.1

ORF Insert Sequence:

ATGGGAAATG GTTCGGTGAA ACCCAAACAC TCCAAGAATC CAGATGGGCA CCCCGGGAAC 
CTCACCGCCA GCGCCCTCCG GAGCAGGGTG ACAGAGCTGG AAAGAGAGCT GAGAAGGAAG 
GATGCTGAGA TCCAGGAGCG GGAATACCAC CTGAAGGAGC TGCGGGAGCA GCTGTCCAAA 
CAGACTGTGG CCATCGCGGA GCTCACGGAG GAGCTCCAGA ACAAGTGCAT CCAGCTGAAC 
AAGCTTCAGG ATGTGGTGCA CCTGCAGGGA GGAAGCCTGC CCCGGGCGTC CCCGGACAAA 
GTGCCTCTTG AGGTTCAGCG GAAGACCTCG GGATTGGTCT CCCTCCACAG CAGGAGGGGA 
GCGAAGGCTG GAGTGTCCGC CGAGCCGACC ACCCGAACCT ATGACCTCAA CAAACCCCCT 
GAATTTTCCT TTGAGAAAGC AAGAGTCAGA AAGGACTCCA GTGAGAAGAA GCTCATTACA 
GATGCCCTTA ATAAAAATCA ATTTCTGAAG AGACTGGATC CTCAGCAGAT CAAAGACATG 
GTGGAATGCA TGTATGGGAG AAACTACCAG CAAGGGAGCT ACATTATTAA GCAAGGAGAA 
CCAGGAAACC ATATCTTTGT ACTGGCAGAG GGTCGACTAG AGGTGTCCCA AGGGGAGAAA 
TTGCTGTCAT CCATCCCTAT GTGGACCACG TTTGGGGAGC TAGCCATTTT ATACAACTGT 
ACAAGAACTG CCTCTGTGAA AGCTATTACC AATGTTAAAA CATGGGCACT AGATCGAGAG 
GTATTCCAGA ATATAATGAG AAGGACAGCT CAAGCTAGAG ATGAACAGTA CAGAAACTTC 
CTCAGAAGTG TATCCTTGCT GAAGAATTTA CCTGAAGATA AACTAACCAA GATCATTGAC 
TGCTTGGAAG TGGAATACTA TGACAAAGGA GATTACATTA TTAGAGAGGG CGAGGAAGGA 
AGTACGTTTT TCATTTTAGC AAAAGGAAAG GTAAAAGTCA CTCAAAGTAC TGAAGGCCAT 
GATCAACCAC AGCTAATAAA AACACTGCAG AAAGGAGAAT ATTTTGGAGA AAAAGCTCTT 
ATCAGTGAAG ATGTCAGATC AGCTAACATT ATCGCCGAGG AAAATGATGT CGCATGCCTG 
GTTATAGATC GAGAAACATT CAACCAAACA GTGGGGACTT TTGAAGAACT CCAGAAATAT 
CTTGAAGGGT ATGTGGCAAA CCTGAACCGG GATGATGAAA AAAGACATGC GAAGAGATCC 
ATGTCTAGCT GGAAGCTGTC CAAAGCACTC TCTTTGGAGA TGATTCAACT GAAGGAGAAA 
GTGGCCAGAT TTTCCTCATC ATCCCCCTTC CAGAACCTTG AGATTATCGC AACACTGGGC 
GTTGGTGGGT TCGGAAGAGT TGAGCTTGTT AAGGTGAAAA ATGAGAACGT TGCTTTTGCT 
ATGAAGTGTA TAAGGAAGAA GCACATCGTT GACACGAAGC AGCAGGAGCA TGTCTACTCA 
GAAAAGAAGA TCCTGGAAGA GCTATGCTCA CCCTTCATTG TGAAATTATA TCGCACCTTC 
AAGGACAATA AGTATGTATA CATGCTTCTG GAGGCCTGCT TAGGTGGGGA GCTGTGGAGT 
ATATTAAGAG ACAGAGGCAG CTTTGATGAA CCCACCTCCA AGTTCTGTGT TGCCTGTGTG 
ACAGAAGCAT TCGATTACCT GCATCGACTA GGTATTATCT ACAGAGACCT GAAACCAGAA 
AACTTAATTC TAGATGCTGA GGGCTATCTT AAATTGGTTG ACTTTGGATT TGCTAAGAAA 
ATAGGATCTG GACAGAAAAC ATGGACGTTC TGTGGAACTC CAGAGTATGT AGCTCCTGAA 
GTCATTCTCA ACAAAGGACA TGACTTCAGT GTGGATTTTT GGTCCCTGGG AATTCTAGTA 
TATGAGCTCC TCACGGGCAA CCCTCCTTTT TCTGGGATTG ACCAAATGAT GACCTACAAT 
CTGATTCTCA AAGGCATTGA AAAAATGGAT TTTCCCAGAA AGATAACAAG ACGACCTGAG 
GATCTGATTC GGAGGCTTTG CAGGCAAAAT CCAACAGAAA GACTGGGAAA TCTGAAGAAT 
GGAATCAATG ACATTAAGAA ACACAGGTGG TTAAATGGTT TTAATTGGGA GGGACTGAAA 
GCACGAAATC TTCCATCACC TTTACAAAGA GAGCTCAGTG GACCCACAGA TCACAGCTAC 
TTTGACAAGT ATCCTCCTGA AAGGGGGGTG CCTCCAGATG AGCTGTCAGG CTGGGACAAA 
GACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A nonsense mutation in cGMP-dependent type II protein kinase (PRKG2) causes dwarfism in American Angus cattle.
Proceedings of the National Academy of Sciences of the United States of America106(46)19250-5(2009 Nov)
Koltes JE,Mishra BP,Kumar D,Kataria RS,Totir LR,Fernando RL,Cobbold R,Steffen D,Coppieters W,Georges M,Reecy JM

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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PRKG2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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