shakB cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	shakB
Entrez Gene ID	33062
Full Name	shaking B
Synonyms	2,3,6,CG12678,CG1321,CG15451,CG32508,CG34358,Dmel\CG34358,Dmel_CG12678,Dmel_CG32508,E81,FBpp0111481,Pas,R-9-29,R9-29,Shak B,Shak-B,ShakB,W3,inx8,l(1)19Eb,l(1)LB21,l(1)R-10-14,l(1)R-10-3,l(1)R-10-7,l(1)R-9-29,l(1)TH73,l(1)W3,l(1)W3b,lincRNA.1031,nj-156,nj156,pas,pass,shB,shak-B,shkB
General protein information	Preferred Names shaking B Names CG34358 gene product from transcript CG34358-RH CG34358-PA CG34358-PC CG34358-PD CG34358-PE CG34358-PF CG34358-PG CG34358-PH CG34358-PI Shaking-B non-jumper-156 passover passover-vital protein section 2 section 3 section 6 shakB-PA shakB-PC shakB-PD shakB-PE shakB-PF shakB-PG shakB-PH shakB-PI
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 19E2-19E3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001258859.2	NP_001245788.1	shaking B, isoform H
NM_001298574.1	NP_001285503.1	shaking B, isoform I
NM_001144750.2	NP_001138222.1	shaking B, isoform F
NM_078701.3	NP_523425.2	shaking B, isoform C
NM_001103568.3	NP_001097038.2	shaking B, isoform E
NM_134566.3	NP_608410.2	shaking B, isoform D
NM_170660.2	NP_728361.1	shaking B, isoform A
NM_001258858.2	NP_001245787.1	shaking B, isoform G

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	shakB	NP_728361.1
Anopheles gambiae (African malaria mosquito)	SHAKB_ANOGA	XP_321635.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Overexpression of ShakB(N+16) in JONs caused the formation of ectopic dye coupling
Title: Shaking B Mediates Synaptic Coupling between Auditory Sensory Neurons and the Giant Fiber of Drosophila melanogaster.

Tryptophan scanning was used to probe the first transmembrane domain (M1) of the Drosophila innexin Shaking-B(Lethal).
Title: Tryptophan scanning mutagenesis of the first transmembrane domain of the innexin Shaking-B(Lethal).

The escape response is impaired in ShakB2 mutants, but they displayed normal voluntary flight initiation. The escape mechanism is subject to developmental modulation following eclosion and the GF system does not underlie voluntary flight.
Title: Ontogeny of flight initiation in the fly Drosophila melanogaster: implications for the giant fibre system.

Seizures caused by bang-sensitive (bas2) and bang-senseless (bss1, bss2 alleles) mutations are not suppressed by shakB2
Title: Seizure suppression by shakB2, a gap junction mutation in Drosophila.

Data show that innexins, including shakB and ogre, are not functionally interchangeable in Drosophila during photoreceptor development.
Title: Gap junction proteins are not interchangeable in development of neural function in the Drosophila visual system.

The following shakB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the shakB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa02215	NM_001298574.1 Latest version!	Drosophila melanogaster shaking B (shakB), transcript variant I, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa02215	NM_001258858.2 Latest version!	Drosophila melanogaster shaking B (shakB), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa02215	NM_001258859.2 Latest version!	Drosophila melanogaster shaking B (shakB), transcript variant H, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa02216	NM_001144750.2 Latest version!	Drosophila melanogaster shaking B (shakB), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OFa03229	NM_078701.3 Latest version!	Drosophila melanogaster shaking B (shakB), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa03230	NM_134566.3 Latest version!	Drosophila melanogaster shaking B (shakB), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa03231	NM_170660.2 Latest version!	Drosophila melanogaster shaking B (shakB), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa03232	NM_001103568.3 Latest version!	Drosophila melanogaster shaking B (shakB), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

shakB ( NM_001258859.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245788.1 Drosophila melanogaster shaking B (shakB), transcript variant H, mRNA.

Price & Availability↑

CloneID	OFa02215
Clone ID Related Accession (Same CDS sequence)	NM_001258859.2 , NM_001298574.1 , NM_001258858.2
Accession Version	NM_001258859.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	shaking B, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_001258859.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGTTTT TACGCGGCGT CTATGCGTTT ATGCAAGTGA GTAGATCATC GGTGTCTCAT 
GTCAAAATCG ATTCACCTGT TTTTCGCCTG CACACCAACG CAACGGTTAT ATTACTGATC 
ACATTCTCGA TTGCTGTGAC GACGCGGCAG TACGTAGGAA ATCCCATAGA CTGTGTACAT 
ACAAGAGACA TTCCCGAAGA TGTTCTCAAC ACATATTGTT GGATACACTC AACGTATACA 
GTGGTCGATG CGTTTATGAA GAAGCAAGGT TCCGAGGTGC CTTTTCCCGG AGTTCACAAT 
TCACAGGGCC GTGGGCCTCT TACAATAAAG CACACAAAAT ATTATCAATG GGTAGCGTTT 
ACACTATTTT TTCAGGCAAT CTTATTTTAT ACACCAAGAT GGCTGTGGAA ATCTTGGGAG 
GGTGGCAAGA TTCATGCGCT CATCATGGAC TTAGACATAG GCATTTGTTC CGAAGCCGAG 
AAAAAACAAA AAAAGAAATT ACTTTTAGAT TATTTGTGGG AAAATTTAAG ATATCACAAT 
TGGTGGGCGT ACAGATATTA CGTGTGTGAG CTGCTCGCCC TTATAAATGT GATAGGTCAA 
ATGTTTCTTA TGAATCGATT TTTCGATGGC GAATTTATAA CATTTGGCCT GAAAGTGATA 
GATTATATGG AGACCGATCA GGAAGATCGC ATGGATCCGA TGATTTACAT ATTTCCCAGA 
ATGACCAAAT GTACATTTTT TAAATATGGT TCCAGTGGGG AGGTGGAGAA ACACGACGCC 
ATTTGCATTT TACCATTAAA CGTTGTTAAT GAGAAGATTT ACATTTTCCT TTGGTTTTGG 
TTTATATTAT TAACGTTTCT CACATTATTA ACGCTAATAT ACAGGGTGGT TATTATATTC 
TCTCCTCGAA TGAGGGTCTA CTTATTTCGT ATGCGATTTA GGTTAGTGCG TCGTGACGCT 
ATTGAAATAA TCGTTCGTCG TTCAAAGATG GGCGATTGGT TTTTGTTGTA TTTACTAGGT 
GAAAACATAG ATACAGTTAT ATTTCGTGAT GTTGTACAGG ACTTAGCGAA TCGTTTAGGA 
CATAACCAAC ACCACAGGGT GCCTGGCTTA AAAGGTGAAA TACAGGATGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245788.1
CDS	2040..3173
Translation	MEFLRGVYAFMQVSRSSVSHVKIDSPVFRLHTNATVILLITFSIAVTTRQYVGNPIDCVHTRDIPEDVLNTYCWIHSTYTVVDAFMKKQGSEVPFPGVHNSQGRGPLTIKHTKYYQWVAFTLFFQAILFYTPRWLWKSWEGGKIHALIMDLDIGICSEAEKKQKKKLLLDYLWENLRYHNWWAYRYYVCELLALINVIGQMFLMNRFFDGEFITFGLKVIDYMETDQEDRMDPMIYIFPRMTKCTFFKYGSSGEVEKHDAICILPLNVVNEKIYIFLWFWFILLTFLTLLTLIYRVVIIFSPRMRVYLFRMRFRLVRRDAIEIIVRRSKMGDWFLLYLLGENIDTVIFRDVVQDLANRLGHNQHHRVPGLKGEIQDA

Target ORF information:

RefSeq Version	NM_001258859.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster shaking B (shakB), transcript variant H, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258859.2

ORF Insert Sequence:

ATGGAGTTTT TACGCGGCGT CTATGCGTTT ATGCAAGTGA GTAGATCATC GGTGTCTCAT 
GTCAAAATCG ATTCACCTGT TTTTCGCCTG CACACCAACG CAACGGTTAT ATTACTGATC 
ACATTCTCGA TTGCTGTGAC GACGCGGCAG TACGTAGGAA ATCCCATAGA CTGTGTACAT 
ACAAGAGACA TTCCCGAAGA TGTTCTCAAC ACATATTGTT GGATACACTC AACGTATACA 
GTGGTCGATG CGTTTATGAA GAAGCAAGGT TCCGAGGTGC CTTTTCCCGG AGTTCACAAT 
TCACAGGGCC GTGGGCCTCT TACAATAAAG CACACAAAAT ATTATCAATG GGTAGCGTTT 
ACACTATTTT TTCAGGCAAT CTTATTTTAT ACACCAAGAT GGCTGTGGAA ATCTTGGGAG 
GGTGGCAAGA TTCATGCGCT CATCATGGAC TTAGACATAG GCATTTGTTC CGAAGCCGAG 
AAAAAACAAA AAAAGAAATT ACTTTTAGAT TATTTGTGGG AAAATTTAAG ATATCACAAT 
TGGTGGGCGT ACAGATATTA CGTGTGTGAG CTGCTCGCCC TTATAAATGT GATAGGTCAA 
ATGTTTCTTA TGAATCGATT TTTCGATGGC GAATTTATAA CATTTGGCCT GAAAGTGATA 
GATTATATGG AGACCGATCA GGAAGATCGC ATGGATCCGA TGATTTACAT ATTTCCCAGA 
ATGACCAAAT GTACATTTTT TAAATATGGT TCCAGTGGGG AGGTGGAGAA ACACGACGCC 
ATTTGCATTT TACCATTAAA CGTTGTTAAT GAGAAGATTT ACATTTTCCT TTGGTTTTGG 
TTTATATTAT TAACGTTTCT CACATTATTA ACGCTAATAT ACAGGGTGGT TATTATATTC 
TCTCCTCGAA TGAGGGTCTA CTTATTTCGT ATGCGATTTA GGTTAGTGCG TCGTGACGCT 
ATTGAAATAA TCGTTCGTCG TTCAAAGATG GGCGATTGGT TTTTGTTGTA TTTACTAGGT 
GAAAACATAG ATACAGTTAT ATTTCGTGAT GTTGTACAGG ACTTAGCGAA TCGTTTAGGA 
CATAACCAAC ACCACAGGGT GCCTGGCTTA AAAGGTGAAA TACAGGATGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shakB ( NM_001298574.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285503.1 Drosophila melanogaster shaking B (shakB), transcript variant I, mRNA.

Price & Availability↑

CloneID	OFa02215
Clone ID Related Accession (Same CDS sequence)	NM_001258859.2 , NM_001298574.1 , NM_001258858.2
Accession Version	NM_001298574.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	shaking B, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGTTTT TACGCGGCGT CTATGCGTTT ATGCAAGTGA GTAGATCATC GGTGTCTCAT 
GTCAAAATCG ATTCACCTGT TTTTCGCCTG CACACCAACG CAACGGTTAT ATTACTGATC 
ACATTCTCGA TTGCTGTGAC GACGCGGCAG TACGTAGGAA ATCCCATAGA CTGTGTACAT 
ACAAGAGACA TTCCCGAAGA TGTTCTCAAC ACATATTGTT GGATACACTC AACGTATACA 
GTGGTCGATG CGTTTATGAA GAAGCAAGGT TCCGAGGTGC CTTTTCCCGG AGTTCACAAT 
TCACAGGGCC GTGGGCCTCT TACAATAAAG CACACAAAAT ATTATCAATG GGTAGCGTTT 
ACACTATTTT TTCAGGCAAT CTTATTTTAT ACACCAAGAT GGCTGTGGAA ATCTTGGGAG 
GGTGGCAAGA TTCATGCGCT CATCATGGAC TTAGACATAG GCATTTGTTC CGAAGCCGAG 
AAAAAACAAA AAAAGAAATT ACTTTTAGAT TATTTGTGGG AAAATTTAAG ATATCACAAT 
TGGTGGGCGT ACAGATATTA CGTGTGTGAG CTGCTCGCCC TTATAAATGT GATAGGTCAA 
ATGTTTCTTA TGAATCGATT TTTCGATGGC GAATTTATAA CATTTGGCCT GAAAGTGATA 
GATTATATGG AGACCGATCA GGAAGATCGC ATGGATCCGA TGATTTACAT ATTTCCCAGA 
ATGACCAAAT GTACATTTTT TAAATATGGT TCCAGTGGGG AGGTGGAGAA ACACGACGCC 
ATTTGCATTT TACCATTAAA CGTTGTTAAT GAGAAGATTT ACATTTTCCT TTGGTTTTGG 
TTTATATTAT TAACGTTTCT CACATTATTA ACGCTAATAT ACAGGGTGGT TATTATATTC 
TCTCCTCGAA TGAGGGTCTA CTTATTTCGT ATGCGATTTA GGTTAGTGCG TCGTGACGCT 
ATTGAAATAA TCGTTCGTCG TTCAAAGATG GGCGATTGGT TTTTGTTGTA TTTACTAGGT 
GAAAACATAG ATACAGTTAT ATTTCGTGAT GTTGTACAGG ACTTAGCGAA TCGTTTAGGA 
CATAACCAAC ACCACAGGGT GCCTGGCTTA AAAGGTGAAA TACAGGATGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285503.1
CDS	2281..3414
Translation	MEFLRGVYAFMQVSRSSVSHVKIDSPVFRLHTNATVILLITFSIAVTTRQYVGNPIDCVHTRDIPEDVLNTYCWIHSTYTVVDAFMKKQGSEVPFPGVHNSQGRGPLTIKHTKYYQWVAFTLFFQAILFYTPRWLWKSWEGGKIHALIMDLDIGICSEAEKKQKKKLLLDYLWENLRYHNWWAYRYYVCELLALINVIGQMFLMNRFFDGEFITFGLKVIDYMETDQEDRMDPMIYIFPRMTKCTFFKYGSSGEVEKHDAICILPLNVVNEKIYIFLWFWFILLTFLTLLTLIYRVVIIFSPRMRVYLFRMRFRLVRRDAIEIIVRRSKMGDWFLLYLLGENIDTVIFRDVVQDLANRLGHNQHHRVPGLKGEIQDA

Target ORF information:

RefSeq Version	NM_001298574.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster shaking B (shakB), transcript variant I, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298574.1

ORF Insert Sequence:

ATGGAGTTTT TACGCGGCGT CTATGCGTTT ATGCAAGTGA GTAGATCATC GGTGTCTCAT 
GTCAAAATCG ATTCACCTGT TTTTCGCCTG CACACCAACG CAACGGTTAT ATTACTGATC 
ACATTCTCGA TTGCTGTGAC GACGCGGCAG TACGTAGGAA ATCCCATAGA CTGTGTACAT 
ACAAGAGACA TTCCCGAAGA TGTTCTCAAC ACATATTGTT GGATACACTC AACGTATACA 
GTGGTCGATG CGTTTATGAA GAAGCAAGGT TCCGAGGTGC CTTTTCCCGG AGTTCACAAT 
TCACAGGGCC GTGGGCCTCT TACAATAAAG CACACAAAAT ATTATCAATG GGTAGCGTTT 
ACACTATTTT TTCAGGCAAT CTTATTTTAT ACACCAAGAT GGCTGTGGAA ATCTTGGGAG 
GGTGGCAAGA TTCATGCGCT CATCATGGAC TTAGACATAG GCATTTGTTC CGAAGCCGAG 
AAAAAACAAA AAAAGAAATT ACTTTTAGAT TATTTGTGGG AAAATTTAAG ATATCACAAT 
TGGTGGGCGT ACAGATATTA CGTGTGTGAG CTGCTCGCCC TTATAAATGT GATAGGTCAA 
ATGTTTCTTA TGAATCGATT TTTCGATGGC GAATTTATAA CATTTGGCCT GAAAGTGATA 
GATTATATGG AGACCGATCA GGAAGATCGC ATGGATCCGA TGATTTACAT ATTTCCCAGA 
ATGACCAAAT GTACATTTTT TAAATATGGT TCCAGTGGGG AGGTGGAGAA ACACGACGCC 
ATTTGCATTT TACCATTAAA CGTTGTTAAT GAGAAGATTT ACATTTTCCT TTGGTTTTGG 
TTTATATTAT TAACGTTTCT CACATTATTA ACGCTAATAT ACAGGGTGGT TATTATATTC 
TCTCCTCGAA TGAGGGTCTA CTTATTTCGT ATGCGATTTA GGTTAGTGCG TCGTGACGCT 
ATTGAAATAA TCGTTCGTCG TTCAAAGATG GGCGATTGGT TTTTGTTGTA TTTACTAGGT 
GAAAACATAG ATACAGTTAT ATTTCGTGAT GTTGTACAGG ACTTAGCGAA TCGTTTAGGA 
CATAACCAAC ACCACAGGGT GCCTGGCTTA AAAGGTGAAA TACAGGATGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shakB ( NM_001258858.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245787.1 Drosophila melanogaster shaking B (shakB), transcript variant G, mRNA.

Price & Availability↑

CloneID	OFa02215
Clone ID Related Accession (Same CDS sequence)	NM_001258859.2 , NM_001298574.1 , NM_001258858.2
Accession Version	NM_001258858.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	shaking B, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001258858.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGTTTT TACGCGGCGT CTATGCGTTT ATGCAAGTGA GTAGATCATC GGTGTCTCAT 
GTCAAAATCG ATTCACCTGT TTTTCGCCTG CACACCAACG CAACGGTTAT ATTACTGATC 
ACATTCTCGA TTGCTGTGAC GACGCGGCAG TACGTAGGAA ATCCCATAGA CTGTGTACAT 
ACAAGAGACA TTCCCGAAGA TGTTCTCAAC ACATATTGTT GGATACACTC AACGTATACA 
GTGGTCGATG CGTTTATGAA GAAGCAAGGT TCCGAGGTGC CTTTTCCCGG AGTTCACAAT 
TCACAGGGCC GTGGGCCTCT TACAATAAAG CACACAAAAT ATTATCAATG GGTAGCGTTT 
ACACTATTTT TTCAGGCAAT CTTATTTTAT ACACCAAGAT GGCTGTGGAA ATCTTGGGAG 
GGTGGCAAGA TTCATGCGCT CATCATGGAC TTAGACATAG GCATTTGTTC CGAAGCCGAG 
AAAAAACAAA AAAAGAAATT ACTTTTAGAT TATTTGTGGG AAAATTTAAG ATATCACAAT 
TGGTGGGCGT ACAGATATTA CGTGTGTGAG CTGCTCGCCC TTATAAATGT GATAGGTCAA 
ATGTTTCTTA TGAATCGATT TTTCGATGGC GAATTTATAA CATTTGGCCT GAAAGTGATA 
GATTATATGG AGACCGATCA GGAAGATCGC ATGGATCCGA TGATTTACAT ATTTCCCAGA 
ATGACCAAAT GTACATTTTT TAAATATGGT TCCAGTGGGG AGGTGGAGAA ACACGACGCC 
ATTTGCATTT TACCATTAAA CGTTGTTAAT GAGAAGATTT ACATTTTCCT TTGGTTTTGG 
TTTATATTAT TAACGTTTCT CACATTATTA ACGCTAATAT ACAGGGTGGT TATTATATTC 
TCTCCTCGAA TGAGGGTCTA CTTATTTCGT ATGCGATTTA GGTTAGTGCG TCGTGACGCT 
ATTGAAATAA TCGTTCGTCG TTCAAAGATG GGCGATTGGT TTTTGTTGTA TTTACTAGGT 
GAAAACATAG ATACAGTTAT ATTTCGTGAT GTTGTACAGG ACTTAGCGAA TCGTTTAGGA 
CATAACCAAC ACCACAGGGT GCCTGGCTTA AAAGGTGAAA TACAGGATGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245787.1
CDS	1176..2309
Translation	MEFLRGVYAFMQVSRSSVSHVKIDSPVFRLHTNATVILLITFSIAVTTRQYVGNPIDCVHTRDIPEDVLNTYCWIHSTYTVVDAFMKKQGSEVPFPGVHNSQGRGPLTIKHTKYYQWVAFTLFFQAILFYTPRWLWKSWEGGKIHALIMDLDIGICSEAEKKQKKKLLLDYLWENLRYHNWWAYRYYVCELLALINVIGQMFLMNRFFDGEFITFGLKVIDYMETDQEDRMDPMIYIFPRMTKCTFFKYGSSGEVEKHDAICILPLNVVNEKIYIFLWFWFILLTFLTLLTLIYRVVIIFSPRMRVYLFRMRFRLVRRDAIEIIVRRSKMGDWFLLYLLGENIDTVIFRDVVQDLANRLGHNQHHRVPGLKGEIQDA

Target ORF information:

RefSeq Version	NM_001258858.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster shaking B (shakB), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258858.2

ORF Insert Sequence:

ATGGAGTTTT TACGCGGCGT CTATGCGTTT ATGCAAGTGA GTAGATCATC GGTGTCTCAT 
GTCAAAATCG ATTCACCTGT TTTTCGCCTG CACACCAACG CAACGGTTAT ATTACTGATC 
ACATTCTCGA TTGCTGTGAC GACGCGGCAG TACGTAGGAA ATCCCATAGA CTGTGTACAT 
ACAAGAGACA TTCCCGAAGA TGTTCTCAAC ACATATTGTT GGATACACTC AACGTATACA 
GTGGTCGATG CGTTTATGAA GAAGCAAGGT TCCGAGGTGC CTTTTCCCGG AGTTCACAAT 
TCACAGGGCC GTGGGCCTCT TACAATAAAG CACACAAAAT ATTATCAATG GGTAGCGTTT 
ACACTATTTT TTCAGGCAAT CTTATTTTAT ACACCAAGAT GGCTGTGGAA ATCTTGGGAG 
GGTGGCAAGA TTCATGCGCT CATCATGGAC TTAGACATAG GCATTTGTTC CGAAGCCGAG 
AAAAAACAAA AAAAGAAATT ACTTTTAGAT TATTTGTGGG AAAATTTAAG ATATCACAAT 
TGGTGGGCGT ACAGATATTA CGTGTGTGAG CTGCTCGCCC TTATAAATGT GATAGGTCAA 
ATGTTTCTTA TGAATCGATT TTTCGATGGC GAATTTATAA CATTTGGCCT GAAAGTGATA 
GATTATATGG AGACCGATCA GGAAGATCGC ATGGATCCGA TGATTTACAT ATTTCCCAGA 
ATGACCAAAT GTACATTTTT TAAATATGGT TCCAGTGGGG AGGTGGAGAA ACACGACGCC 
ATTTGCATTT TACCATTAAA CGTTGTTAAT GAGAAGATTT ACATTTTCCT TTGGTTTTGG 
TTTATATTAT TAACGTTTCT CACATTATTA ACGCTAATAT ACAGGGTGGT TATTATATTC 
TCTCCTCGAA TGAGGGTCTA CTTATTTCGT ATGCGATTTA GGTTAGTGCG TCGTGACGCT 
ATTGAAATAA TCGTTCGTCG TTCAAAGATG GGCGATTGGT TTTTGTTGTA TTTACTAGGT 
GAAAACATAG ATACAGTTAT ATTTCGTGAT GTTGTACAGG ACTTAGCGAA TCGTTTAGGA 
CATAACCAAC ACCACAGGGT GCCTGGCTTA AAAGGTGAAA TACAGGATGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shakB ( NM_001144750.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001138222.1 Drosophila melanogaster shaking B (shakB), transcript variant F, mRNA.

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CloneID	OFa02216
Clone ID Related Accession (Same CDS sequence)	NM_001144750.2
Accession Version	NM_001144750.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 879bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	shaking B, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001144750.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGAAGC AAGGTTCCGA GGTGCCTTTT CCCGGAGTTC ACAATTCACA GGGCCGTGGG 
CCTCTTACAA TAAAGCACAC AAAATATTAT CAATGGGTAG CGTTTACACT ATTTTTTCAG 
GCAATCTTAT TTTATACACC AAGATGGCTG TGGAAATCTT GGGAGGGTGG CAAGATTCAT 
GCGCTCATCA TGGACTTAGA CATAGGCATT TGTTCCGAAG CCGAGAAAAA ACAAAAAAAG 
AAATTACTTT TAGATTATTT GTGGGAAAAT TTAAGATATC ACAATTGGTG GGCGTACAGA 
TATTACGTGT GTGAGCTGCT CGCCCTTATA AATGTGATAG GTCAAATGTT TCTTATGAAT 
CGATTTTTCG ATGGCGAATT TATAACATTT GGCCTGAAAG TGATAGATTA TATGGAGACC 
GATCAGGAAG ATCGCATGGA TCCGATGATT TACATATTTC CCAGAATGAC CAAATGTACA 
TTTTTTAAAT ATGGTTCCAG TGGGGAGGTG GAGAAACACG ACGCCATTTG CATTTTACCA 
TTAAACGTTG TTAATGAGAA GATTTACATT TTCCTTTGGT TTTGGTTTAT ATTATTAACG 
TTTCTCACAT TATTAACGCT AATATACAGG GTGGTTATTA TATTCTCTCC TCGAATGAGG 
GTCTACTTAT TTCGTATGCG ATTTAGGTTA GTGCGTCGTG ACGCTATTGA AATAATCGTT 
CGTCGTTCAA AGATGGGCGA TTGGTTTTTG TTGTATTTAC TAGGTGAAAA CATAGATACA 
GTTATATTTC GTGATGTTGT ACAGGACTTA GCGAATCGTT TAGGACATAA CCAACACCAC 
AGGGTGCCTG GCTTAAAAGG TGAAATACAG GATGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138222.1
CDS	320..1198
Translation	MKKQGSEVPFPGVHNSQGRGPLTIKHTKYYQWVAFTLFFQAILFYTPRWLWKSWEGGKIHALIMDLDIGICSEAEKKQKKKLLLDYLWENLRYHNWWAYRYYVCELLALINVIGQMFLMNRFFDGEFITFGLKVIDYMETDQEDRMDPMIYIFPRMTKCTFFKYGSSGEVEKHDAICILPLNVVNEKIYIFLWFWFILLTFLTLLTLIYRVVIIFSPRMRVYLFRMRFRLVRRDAIEIIVRRSKMGDWFLLYLLGENIDTVIFRDVVQDLANRLGHNQHHRVPGLKGEIQDA

Target ORF information:

RefSeq Version	NM_001144750.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster shaking B (shakB), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144750.2

ORF Insert Sequence:

ATGAAGAAGC AAGGTTCCGA GGTGCCTTTT CCCGGAGTTC ACAATTCACA GGGCCGTGGG 
CCTCTTACAA TAAAGCACAC AAAATATTAT CAATGGGTAG CGTTTACACT ATTTTTTCAG 
GCAATCTTAT TTTATACACC AAGATGGCTG TGGAAATCTT GGGAGGGTGG CAAGATTCAT 
GCGCTCATCA TGGACTTAGA CATAGGCATT TGTTCCGAAG CCGAGAAAAA ACAAAAAAAG 
AAATTACTTT TAGATTATTT GTGGGAAAAT TTAAGATATC ACAATTGGTG GGCGTACAGA 
TATTACGTGT GTGAGCTGCT CGCCCTTATA AATGTGATAG GTCAAATGTT TCTTATGAAT 
CGATTTTTCG ATGGCGAATT TATAACATTT GGCCTGAAAG TGATAGATTA TATGGAGACC 
GATCAGGAAG ATCGCATGGA TCCGATGATT TACATATTTC CCAGAATGAC CAAATGTACA 
TTTTTTAAAT ATGGTTCCAG TGGGGAGGTG GAGAAACACG ACGCCATTTG CATTTTACCA 
TTAAACGTTG TTAATGAGAA GATTTACATT TTCCTTTGGT TTTGGTTTAT ATTATTAACG 
TTTCTCACAT TATTAACGCT AATATACAGG GTGGTTATTA TATTCTCTCC TCGAATGAGG 
GTCTACTTAT TTCGTATGCG ATTTAGGTTA GTGCGTCGTG ACGCTATTGA AATAATCGTT 
CGTCGTTCAA AGATGGGCGA TTGGTTTTTG TTGTATTTAC TAGGTGAAAA CATAGATACA 
GTTATATTTC GTGATGTTGT ACAGGACTTA GCGAATCGTT TAGGACATAA CCAACACCAC 
AGGGTGCCTG GCTTAAAAGG TGAAATACAG GATGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shakB ( NM_078701.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523425.2 Drosophila melanogaster shaking B (shakB), transcript variant C, mRNA.

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CloneID	OFa03229
Clone ID Related Accession (Same CDS sequence)	NM_078701.3
Accession Version	NM_078701.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1086bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	shaking B, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On May 8, 2012 this sequence version replaced NM_078701.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGTCTC ATGTCAAAAT CGATTCACCT GTTTTTCGCC TGCACACCAA CGCAACGGTT 
ATATTACTGA TCACATTCTC GATTGCTGTG ACGACGCGGC AGTACGTAGG AAATCCCATA 
GACTGTGTAC ATACAAGAGA CATTCCCGAA GATGTTCTCA ACACATATTG TTGGATACAC 
TCAACGTATA CAGTGGTCGA TGCGTTTATG AAGAAGCAAG GTTCCGAGGT GCCTTTTCCC 
GGAGTTCACA ATTCACAGGG CCGTGGGCCT CTTACAATAA AGCACACAAA ATATTATCAA 
TGGGTAGCGT TTACACTATT TTTTCAGGCA ATCTTATTTT ATACACCAAG ATGGCTGTGG 
AAATCTTGGG AGGGTGGCAA GATTCATGCG CTCATCATGG ACTTAGACAT AGGCATTTGT 
TCCGAAGCCG AGAAAAAACA AAAAAAGAAA TTACTTTTAG ATTATTTGTG GGAAAATTTA 
AGATATCACA ATTGGTGGGC GTACAGATAT TACGTGTGTG AGCTGCTCGC CCTTATAAAT 
GTGATAGGTC AAATGTTTCT TATGAATCGA TTTTTCGATG GCGAATTTAT AACATTTGGC 
CTGAAAGTGA TAGATTATAT GGAGACCGAT CAGGAAGATC GCATGGATCC GATGATTTAC 
ATATTTCCCA GAATGACCAA ATGTACATTT TTTAAATATG GTTCCAGTGG GGAGGTGGAG 
AAACACGACG CCATTTGCAT TTTACCATTA AACGTTGTTA ATGAGAAGAT TTACATTTTC 
CTTTGGTTTT GGTTTATATT ATTAACGTTT CTCACATTAT TAACGCTAAT ATACAGGGTG 
GTTATTATAT TCTCTCCTCG AATGAGGGTC TACTTATTTC GTATGCGATT TAGGTTAGTG 
CGTCGTGACG CTATTGAAAT AATCGTTCGT CGTTCAAAGA TGGGCGATTG GTTTTTGTTG 
TATTTACTAG GTGAAAACAT AGATACAGTT ATATTTCGTG ATGTTGTACA GGACTTAGCG 
AATCGTTTAG GACATAACCA ACACCACAGG GTGCCTGGCT TAAAAGGTGA AATACAGGAT 
GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523425.2
CDS	1520..2605
Translation	MVSHVKIDSPVFRLHTNATVILLITFSIAVTTRQYVGNPIDCVHTRDIPEDVLNTYCWIHSTYTVVDAFMKKQGSEVPFPGVHNSQGRGPLTIKHTKYYQWVAFTLFFQAILFYTPRWLWKSWEGGKIHALIMDLDIGICSEAEKKQKKKLLLDYLWENLRYHNWWAYRYYVCELLALINVIGQMFLMNRFFDGEFITFGLKVIDYMETDQEDRMDPMIYIFPRMTKCTFFKYGSSGEVEKHDAICILPLNVVNEKIYIFLWFWFILLTFLTLLTLIYRVVIIFSPRMRVYLFRMRFRLVRRDAIEIIVRRSKMGDWFLLYLLGENIDTVIFRDVVQDLANRLGHNQHHRVPGLKGEIQDA

Target ORF information:

RefSeq Version	NM_078701.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster shaking B (shakB), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078701.3

ORF Insert Sequence:

ATGGTGTCTC ATGTCAAAAT CGATTCACCT GTTTTTCGCC TGCACACCAA CGCAACGGTT 
ATATTACTGA TCACATTCTC GATTGCTGTG ACGACGCGGC AGTACGTAGG AAATCCCATA 
GACTGTGTAC ATACAAGAGA CATTCCCGAA GATGTTCTCA ACACATATTG TTGGATACAC 
TCAACGTATA CAGTGGTCGA TGCGTTTATG AAGAAGCAAG GTTCCGAGGT GCCTTTTCCC 
GGAGTTCACA ATTCACAGGG CCGTGGGCCT CTTACAATAA AGCACACAAA ATATTATCAA 
TGGGTAGCGT TTACACTATT TTTTCAGGCA ATCTTATTTT ATACACCAAG ATGGCTGTGG 
AAATCTTGGG AGGGTGGCAA GATTCATGCG CTCATCATGG ACTTAGACAT AGGCATTTGT 
TCCGAAGCCG AGAAAAAACA AAAAAAGAAA TTACTTTTAG ATTATTTGTG GGAAAATTTA 
AGATATCACA ATTGGTGGGC GTACAGATAT TACGTGTGTG AGCTGCTCGC CCTTATAAAT 
GTGATAGGTC AAATGTTTCT TATGAATCGA TTTTTCGATG GCGAATTTAT AACATTTGGC 
CTGAAAGTGA TAGATTATAT GGAGACCGAT CAGGAAGATC GCATGGATCC GATGATTTAC 
ATATTTCCCA GAATGACCAA ATGTACATTT TTTAAATATG GTTCCAGTGG GGAGGTGGAG 
AAACACGACG CCATTTGCAT TTTACCATTA AACGTTGTTA ATGAGAAGAT TTACATTTTC 
CTTTGGTTTT GGTTTATATT ATTAACGTTT CTCACATTAT TAACGCTAAT ATACAGGGTG 
GTTATTATAT TCTCTCCTCG AATGAGGGTC TACTTATTTC GTATGCGATT TAGGTTAGTG 
CGTCGTGACG CTATTGAAAT AATCGTTCGT CGTTCAAAGA TGGGCGATTG GTTTTTGTTG 
TATTTACTAG GTGAAAACAT AGATACAGTT ATATTTCGTG ATGTTGTACA GGACTTAGCG 
AATCGTTTAG GACATAACCA ACACCACAGG GTGCCTGGCT TAAAAGGTGA AATACAGGAT 
GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shakB ( NM_134566.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_608410.2 Drosophila melanogaster shaking B (shakB), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa03230
Clone ID Related Accession (Same CDS sequence)	NM_134566.3
Accession Version	NM_134566.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	shaking B, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_134566.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTATAAGC CGGACACGCT CACGCGCCGC GGCAGCCTGA GATCCCTGCG CAGCGCCCCG 
CTCCTGAGCA CCGTGCTCGA AAGCACGCTT TCACTGACCC GCATTCACCC GATCGCCTCC 
TTGGAGCTAC CCGGCCTCGA CTATGCGGTC CACTCGCAGT CGGCCTTTGG CGCCTACGGG 
CTAGCCCACC CCCGGGATCT AGCCACCTGC ACCTCGTTGC GCAGCGGCCT TGCGGCGATC 
ACCGCGGCGT CCGCTTCCGC CGCCGGCGGC GTTTCCCAGT CGCAGAGCGC CCTCCTCGGA 
AGGTACGGGC CCAACGCCTC GATTCGCCAC GGAGAAAGAA AGATTGTCCA GCCAAAACGG 
GTTCTATCAC GCAAACTAAA GCCACATCTT GTTGCGGACA CCGTAAAGCA GTATATAAGT 
CGGGCCCAAC GCACAACCAA AAAGGGTTCT CAAGAGCAGC AAAATATGGA GTTTTTACGC 
GGCGTCTATG CGTTTATGCA AGTGAGTAGA TCATCGGTGT CTCATGTCAA AATCGATTCA 
CCTGTTTTTC GCCTGCACAC CAACGCAACG GTTATATTAC TGATCACATT CTCGATTGCT 
GTGACGACGC GGCAGTACGT AGGAAATCCC ATAGACTGTG TACATACAAG AGACATTCCC 
GAAGATGTTC TCAACACATA TTGTTGGATA CACTCAACGT ATACAGTGGT CGATGCGTTT 
ATGAAGAAGC AAGGTTCCGA GGTGCCTTTT CCCGGAGTTC ACAATTCACA GGGCCGTGGG 
CCTCTTACAA TAAAGCACAC AAAATATTAT CAATGGGTAG CGTTTACACT ATTTTTTCAG 
GCAATCTTAT TTTATACACC AAGATGGCTG TGGAAATCTT GGGAGGGTGG CAAGATTCAT 
GCGCTCATCA TGGACTTAGA CATAGGCATT TGTTCCGAAG CCGAGAAAAA ACAAAAAAAG 
AAATTACTTT TAGATTATTT GTGGGAAAAT TTAAGATATC ACAATTGGTG GGCGTACAGA 
TATTACGTGT GTGAGCTGCT CGCCCTTATA AATGTGATAG GTCAAATGTT TCTTATGAAT 
CGATTTTTCG ATGGCGAATT TATAACATTT GGCCTGAAAG TGATAGATTA TATGGAGACC 
GATCAGGAAG ATCGCATGGA TCCGATGATT TACATATTTC CCAGAATGAC CAAATGTACA 
TTTTTTAAAT ATGGTTCCAG TGGGGAGGTG GAGAAACACG ACGCCATTTG CATTTTACCA 
TTAAACGTTG TTAATGAGAA GATTTACATT TTCCTTTGGT TTTGGTTTAT ATTATTAACG 
TTTCTCACAT TATTAACGCT AATATACAGG GTGGTTATTA TATTCTCTCC TCGAATGAGG 
GTCTACTTAT TTCGTATGCG ATTTAGGTTA GTGCGTCGTG ACGCTATTGA AATAATCGTT 
CGTCGTTCAA AGATGGGCGA TTGGTTTTTG TTGTATTTAC TAGGTGAAAA CATAGATACA 
GTTATATTTC GTGATGTTGT ACAGGACTTA GCGAATCGTT TAGGACATAA CCAACACCAC 
AGGGTGCCTG GCTTAAAAGG TGAAATACAG GATGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_608410.2
CDS	111..1709
Translation	MYKPDTLTRRGSLRSLRSAPLLSTVLESTLSLTRIHPIASLELPGLDYAVHSQSAFGAYGLAHPRDLATCTSLRSGLAAITAASASAAGGVSQSQSALLGRYGPNASIRHGERKIVQPKRVLSRKLKPHLVADTVKQYISRAQRTTKKGSQEQQNMEFLRGVYAFMQVSRSSVSHVKIDSPVFRLHTNATVILLITFSIAVTTRQYVGNPIDCVHTRDIPEDVLNTYCWIHSTYTVVDAFMKKQGSEVPFPGVHNSQGRGPLTIKHTKYYQWVAFTLFFQAILFYTPRWLWKSWEGGKIHALIMDLDIGICSEAEKKQKKKLLLDYLWENLRYHNWWAYRYYVCELLALINVIGQMFLMNRFFDGEFITFGLKVIDYMETDQEDRMDPMIYIFPRMTKCTFFKYGSSGEVEKHDAICILPLNVVNEKIYIFLWFWFILLTFLTLLTLIYRVVIIFSPRMRVYLFRMRFRLVRRDAIEIIVRRSKMGDWFLLYLLGENIDTVIFRDVVQDLANRLGHNQHHRVPGLKGEIQDA

Target ORF information:

RefSeq Version	NM_134566.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster shaking B (shakB), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134566.3

ORF Insert Sequence:

ATGTATAAGC CGGACACGCT CACGCGCCGC GGCAGCCTGA GATCCCTGCG CAGCGCCCCG 
CTCCTGAGCA CCGTGCTCGA AAGCACGCTT TCACTGACCC GCATTCACCC GATCGCCTCC 
TTGGAGCTAC CCGGCCTCGA CTATGCGGTC CACTCGCAGT CGGCCTTTGG CGCCTACGGG 
CTAGCCCACC CCCGGGATCT AGCCACCTGC ACCTCGTTGC GCAGCGGCCT TGCGGCGATC 
ACCGCGGCGT CCGCTTCCGC CGCCGGCGGC GTTTCCCAGT CGCAGAGCGC CCTCCTCGGA 
AGGTACGGGC CCAACGCCTC GATTCGCCAC GGAGAAAGAA AGATTGTCCA GCCAAAACGG 
GTTCTATCAC GCAAACTAAA GCCACATCTT GTTGCGGACA CCGTAAAGCA GTATATAAGT 
CGGGCCCAAC GCACAACCAA AAAGGGTTCT CAAGAGCAGC AAAATATGGA GTTTTTACGC 
GGCGTCTATG CGTTTATGCA AGTGAGTAGA TCATCGGTGT CTCATGTCAA AATCGATTCA 
CCTGTTTTTC GCCTGCACAC CAACGCAACG GTTATATTAC TGATCACATT CTCGATTGCT 
GTGACGACGC GGCAGTACGT AGGAAATCCC ATAGACTGTG TACATACAAG AGACATTCCC 
GAAGATGTTC TCAACACATA TTGTTGGATA CACTCAACGT ATACAGTGGT CGATGCGTTT 
ATGAAGAAGC AAGGTTCCGA GGTGCCTTTT CCCGGAGTTC ACAATTCACA GGGCCGTGGG 
CCTCTTACAA TAAAGCACAC AAAATATTAT CAATGGGTAG CGTTTACACT ATTTTTTCAG 
GCAATCTTAT TTTATACACC AAGATGGCTG TGGAAATCTT GGGAGGGTGG CAAGATTCAT 
GCGCTCATCA TGGACTTAGA CATAGGCATT TGTTCCGAAG CCGAGAAAAA ACAAAAAAAG 
AAATTACTTT TAGATTATTT GTGGGAAAAT TTAAGATATC ACAATTGGTG GGCGTACAGA 
TATTACGTGT GTGAGCTGCT CGCCCTTATA AATGTGATAG GTCAAATGTT TCTTATGAAT 
CGATTTTTCG ATGGCGAATT TATAACATTT GGCCTGAAAG TGATAGATTA TATGGAGACC 
GATCAGGAAG ATCGCATGGA TCCGATGATT TACATATTTC CCAGAATGAC CAAATGTACA 
TTTTTTAAAT ATGGTTCCAG TGGGGAGGTG GAGAAACACG ACGCCATTTG CATTTTACCA 
TTAAACGTTG TTAATGAGAA GATTTACATT TTCCTTTGGT TTTGGTTTAT ATTATTAACG 
TTTCTCACAT TATTAACGCT AATATACAGG GTGGTTATTA TATTCTCTCC TCGAATGAGG 
GTCTACTTAT TTCGTATGCG ATTTAGGTTA GTGCGTCGTG ACGCTATTGA AATAATCGTT 
CGTCGTTCAA AGATGGGCGA TTGGTTTTTG TTGTATTTAC TAGGTGAAAA CATAGATACA 
GTTATATTTC GTGATGTTGT ACAGGACTTA GCGAATCGTT TAGGACATAA CCAACACCAC 
AGGGTGCCTG GCTTAAAAGG TGAAATACAG GATGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shakB ( NM_170660.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728361.1 Drosophila melanogaster shaking B (shakB), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa03231
Clone ID Related Accession (Same CDS sequence)	NM_170660.2
Accession Version	NM_170660.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	shaking B, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_170660.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTAGATA TATTTCGTGG ATTGAAAAAC CTTGTAAAGG TCAGTCACGT TAAAACAGAT 
TCGATAGTAT TCCGCCTGCA TTACTCAATA ACTGTGATGA TTCTGATGTC ATTCTCGCTA 
ATTATAACCA CGCGCCAGTA CGTTGGCAAT CCGATCGATT GTGTGCACAC CAAAGATATT 
CCAGAGGATG TGCTCAACAC TTACTGCTGG ATCCAGTCCA CCTACACGCT CAAGAGCCTC 
TTCCTGAAGA AGCAGGGCGT GTCGGTTCCA TATCCGGGCA TCGGCAACTC CGACGGTGAT 
CCGGCGGACA AGAAGCACTA CAAGTACTAC CAGTGGGTCT GCTTCTGCCT CTTCTTTCAG 
GCAATCTTAT TTTATACACC AAGATGGCTG TGGAAATCTT GGGAGGGTGG CAAGATTCAT 
GCGCTCATCA TGGACTTAGA CATAGGCATT TGTTCCGAAG CCGAGAAAAA ACAAAAAAAG 
AAATTACTTT TAGATTATTT GTGGGAAAAT TTAAGATATC ACAATTGGTG GGCGTACAGA 
TATTACGTGT GTGAGCTGCT CGCCCTTATA AATGTGATAG GTCAAATGTT TCTTATGAAT 
CGATTTTTCG ATGGCGAATT TATAACATTT GGCCTGAAAG TGATAGATTA TATGGAGACC 
GATCAGGAAG ATCGCATGGA TCCGATGATT TACATATTTC CCAGAATGAC CAAATGTACA 
TTTTTTAAAT ATGGTTCCAG TGGGGAGGTG GAGAAACACG ACGCCATTTG CATTTTACCA 
TTAAACGTTG TTAATGAGAA GATTTACATT TTCCTTTGGT TTTGGTTTAT ATTATTAACG 
TTTCTCACAT TATTAACGCT AATATACAGG GTGGTTATTA TATTCTCTCC TCGAATGAGG 
GTCTACTTAT TTCGTATGCG ATTTAGGTTA GTGCGTCGTG ACGCTATTGA AATAATCGTT 
CGTCGTTCAA AGATGGGCGA TTGGTTTTTG TTGTATTTAC TAGGTGAAAA CATAGATACA 
GTTATATTTC GTGATGTTGT ACAGGACTTA GCGAATCGTT TAGGACATAA CCAACACCAC 
AGGGTGCCTG GCTTAAAAGG TGAAATACAG GATGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728361.1
CDS	839..1957
Translation	MLDIFRGLKNLVKVSHVKTDSIVFRLHYSITVMILMSFSLIITTRQYVGNPIDCVHTKDIPEDVLNTYCWIQSTYTLKSLFLKKQGVSVPYPGIGNSDGDPADKKHYKYYQWVCFCLFFQAILFYTPRWLWKSWEGGKIHALIMDLDIGICSEAEKKQKKKLLLDYLWENLRYHNWWAYRYYVCELLALINVIGQMFLMNRFFDGEFITFGLKVIDYMETDQEDRMDPMIYIFPRMTKCTFFKYGSSGEVEKHDAICILPLNVVNEKIYIFLWFWFILLTFLTLLTLIYRVVIIFSPRMRVYLFRMRFRLVRRDAIEIIVRRSKMGDWFLLYLLGENIDTVIFRDVVQDLANRLGHNQHHRVPGLKGEIQDA

Target ORF information:

RefSeq Version	NM_170660.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster shaking B (shakB), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170660.2

ORF Insert Sequence:

ATGTTAGATA TATTTCGTGG ATTGAAAAAC CTTGTAAAGG TCAGTCACGT TAAAACAGAT 
TCGATAGTAT TCCGCCTGCA TTACTCAATA ACTGTGATGA TTCTGATGTC ATTCTCGCTA 
ATTATAACCA CGCGCCAGTA CGTTGGCAAT CCGATCGATT GTGTGCACAC CAAAGATATT 
CCAGAGGATG TGCTCAACAC TTACTGCTGG ATCCAGTCCA CCTACACGCT CAAGAGCCTC 
TTCCTGAAGA AGCAGGGCGT GTCGGTTCCA TATCCGGGCA TCGGCAACTC CGACGGTGAT 
CCGGCGGACA AGAAGCACTA CAAGTACTAC CAGTGGGTCT GCTTCTGCCT CTTCTTTCAG 
GCAATCTTAT TTTATACACC AAGATGGCTG TGGAAATCTT GGGAGGGTGG CAAGATTCAT 
GCGCTCATCA TGGACTTAGA CATAGGCATT TGTTCCGAAG CCGAGAAAAA ACAAAAAAAG 
AAATTACTTT TAGATTATTT GTGGGAAAAT TTAAGATATC ACAATTGGTG GGCGTACAGA 
TATTACGTGT GTGAGCTGCT CGCCCTTATA AATGTGATAG GTCAAATGTT TCTTATGAAT 
CGATTTTTCG ATGGCGAATT TATAACATTT GGCCTGAAAG TGATAGATTA TATGGAGACC 
GATCAGGAAG ATCGCATGGA TCCGATGATT TACATATTTC CCAGAATGAC CAAATGTACA 
TTTTTTAAAT ATGGTTCCAG TGGGGAGGTG GAGAAACACG ACGCCATTTG CATTTTACCA 
TTAAACGTTG TTAATGAGAA GATTTACATT TTCCTTTGGT TTTGGTTTAT ATTATTAACG 
TTTCTCACAT TATTAACGCT AATATACAGG GTGGTTATTA TATTCTCTCC TCGAATGAGG 
GTCTACTTAT TTCGTATGCG ATTTAGGTTA GTGCGTCGTG ACGCTATTGA AATAATCGTT 
CGTCGTTCAA AGATGGGCGA TTGGTTTTTG TTGTATTTAC TAGGTGAAAA CATAGATACA 
GTTATATTTC GTGATGTTGT ACAGGACTTA GCGAATCGTT TAGGACATAA CCAACACCAC 
AGGGTGCCTG GCTTAAAAGG TGAAATACAG GATGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

shakB ( NM_001103568.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097038.2 Drosophila melanogaster shaking B (shakB), transcript variant E, mRNA.

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CloneID	OFa03232
Clone ID Related Accession (Same CDS sequence)	NM_001103568.3
Accession Version	NM_001103568.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	shaking B, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001103568.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGGGGC GACATTGTCA TGTGCCAAAG CAAACACACC ATATACAATT TGCACAACTT 
GCCCAATCCC CAGCTGTCTG TGGATTGTCC GAATCCGCTG CTAATTTACG ATACAAGTTA 
TTATTAATTT GTCATCGGGT CGGCATTAAA ATCCCCCGGC AAAAGAAAAT ACCGACAACT 
TATAATGAGC AGGCAATCTT ATTTTATACA CCAAGATGGC TGTGGAAATC TTGGGAGGGT 
GGCAAGATTC ATGCGCTCAT CATGGACTTA GACATAGGCA TTTGTTCCGA AGCCGAGAAA 
AAACAAAAAA AGAAATTACT TTTAGATTAT TTGTGGGAAA ATTTAAGATA TCACAATTGG 
TGGGCGTACA GATATTACGT GTGTGAGCTG CTCGCCCTTA TAAATGTGAT AGGTCAAATG 
TTTCTTATGA ATCGATTTTT CGATGGCGAA TTTATAACAT TTGGCCTGAA AGTGATAGAT 
TATATGGAGA CCGATCAGGA AGATCGCATG GATCCGATGA TTTACATATT TCCCAGAATG 
ACCAAATGTA CATTTTTTAA ATATGGTTCC AGTGGGGAGG TGGAGAAACA CGACGCCATT 
TGCATTTTAC CATTAAACGT TGTTAATGAG AAGATTTACA TTTTCCTTTG GTTTTGGTTT 
ATATTATTAA CGTTTCTCAC ATTATTAACG CTAATATACA GGGTGGTTAT TATATTCTCT 
CCTCGAATGA GGGTCTACTT ATTTCGTATG CGATTTAGGT TAGTGCGTCG TGACGCTATT 
GAAATAATCG TTCGTCGTTC AAAGATGGGC GATTGGTTTT TGTTGTATTT ACTAGGTGAA 
AACATAGATA CAGTTATATT TCGTGATGTT GTACAGGACT TAGCGAATCG TTTAGGACAT 
AACCAACACC ACAGGGTGCC TGGCTTAAAA GGTGAAATAC AGGATGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097038.2
CDS	1507..2457
Translation	MMGRHCHVPKQTHHIQFAQLAQSPAVCGLSESAANLRYKLLLICHRVGIKIPRQKKIPTTYNEQAILFYTPRWLWKSWEGGKIHALIMDLDIGICSEAEKKQKKKLLLDYLWENLRYHNWWAYRYYVCELLALINVIGQMFLMNRFFDGEFITFGLKVIDYMETDQEDRMDPMIYIFPRMTKCTFFKYGSSGEVEKHDAICILPLNVVNEKIYIFLWFWFILLTFLTLLTLIYRVVIIFSPRMRVYLFRMRFRLVRRDAIEIIVRRSKMGDWFLLYLLGENIDTVIFRDVVQDLANRLGHNQHHRVPGLKGEIQDA

Target ORF information:

RefSeq Version	NM_001103568.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster shaking B (shakB), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103568.3

ORF Insert Sequence:

ATGATGGGGC GACATTGTCA TGTGCCAAAG CAAACACACC ATATACAATT TGCACAACTT 
GCCCAATCCC CAGCTGTCTG TGGATTGTCC GAATCCGCTG CTAATTTACG ATACAAGTTA 
TTATTAATTT GTCATCGGGT CGGCATTAAA ATCCCCCGGC AAAAGAAAAT ACCGACAACT 
TATAATGAGC AGGCAATCTT ATTTTATACA CCAAGATGGC TGTGGAAATC TTGGGAGGGT 
GGCAAGATTC ATGCGCTCAT CATGGACTTA GACATAGGCA TTTGTTCCGA AGCCGAGAAA 
AAACAAAAAA AGAAATTACT TTTAGATTAT TTGTGGGAAA ATTTAAGATA TCACAATTGG 
TGGGCGTACA GATATTACGT GTGTGAGCTG CTCGCCCTTA TAAATGTGAT AGGTCAAATG 
TTTCTTATGA ATCGATTTTT CGATGGCGAA TTTATAACAT TTGGCCTGAA AGTGATAGAT 
TATATGGAGA CCGATCAGGA AGATCGCATG GATCCGATGA TTTACATATT TCCCAGAATG 
ACCAAATGTA CATTTTTTAA ATATGGTTCC AGTGGGGAGG TGGAGAAACA CGACGCCATT 
TGCATTTTAC CATTAAACGT TGTTAATGAG AAGATTTACA TTTTCCTTTG GTTTTGGTTT 
ATATTATTAA CGTTTCTCAC ATTATTAACG CTAATATACA GGGTGGTTAT TATATTCTCT 
CCTCGAATGA GGGTCTACTT ATTTCGTATG CGATTTAGGT TAGTGCGTCG TGACGCTATT 
GAAATAATCG TTCGTCGTTC AAAGATGGGC GATTGGTTTT TGTTGTATTT ACTAGGTGAA 
AACATAGATA CAGTTATATT TCGTGATGTT GTACAGGACT TAGCGAATCG TTTAGGACAT 
AACCAACACC ACAGGGTGCC TGGCTTAAAA GGTGAAATAC AGGATGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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shakB cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

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