CG32533 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG32533
Entrez Gene ID	32933
Full Name	CG32533 gene product from transcript CG32533-RA
Synonyms	CG12211,CG14198,Dmel\CG32533,cg12211
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 18C7-18C7

mRNA and Protein(s)

mRNA	Protein	Name
NM_167649.2	NP_728244.1	uncharacterized protein, isoform A
NM_001298508.1	NP_001285437.1	uncharacterized protein, isoform B

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	DHX34	XP_005616451.1
Caenorhabditis elegans (roundworm)	smgl-2	NP_500405.3
Homo sapiens (human)	DHX34	NP_055496.2
	LOC718100	XP_002801360.1
Bos taurus (cattle)	DHX34	NP_001179053.1
Drosophila melanogaster (fruit fly)	CG32533	NP_728244.1
Pan troglodytes (chimpanzee)	DHX34	XP_001169079.1
Mus musculus (house mouse)	Dhx34	NP_001272860.1
Rattus norvegicus (Norway rat)	Dhx34	NP_001258381.1
Danio rerio (zebrafish)	dhx34	NP_001121805.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001338	XP_321806.4
Xenopus tropicalis (tropical clawed frog)	dhx34	NP_001121459.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG32533 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG32533 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa02118	NM_167649.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG32533), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa02118	NM_001298508.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG32533), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG32533 ( NM_167649.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728244.1 Drosophila melanogaster uncharacterized protein, transcript variant A (CG32533), mRNA.

Price & Availability↑

CloneID	OFa02118
Clone ID Related Accession (Same CDS sequence)	NM_167649.2 , NM_001298508.1
Accession Version	NM_167649.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167649.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTCGGA TAAAGAAGAA GGCAGATGAA CATGCCTCCA GCCGCCGCAG GACTCAGATC 
AATCTGATCG AGTTCAGTTT CTTGGATCAC AAGGCGGAGC TCGTGGGCCT GCTGCAGGCC 
AAGAGCCGCC AGGTAGGCAG GAGTCTGGTG GAGAACGAGG CTGACTTCTG GAAGTTCGTG 
GGCAAGTACG AGGCTATGAT GCGCTCCACG GGCCAGCCCG TGCTGCCGCC TCGACTCGCG 
GAGGCGGAGC TGAGCAGCAT GCAGCCGTAT CACCGCAGCA AGCACTTGGC ATTGCGGCTG 
GACGCTGACG AACTGCTTCA GGAGGCGCGC GACCAAATGC AGTCCCAGTT TCGTCATATC 
TTGCTGGTGT ACTTAGACTT CCGCCAGACG GAAAAGTTCC AGCGTATCCG AAAGCTGCGG 
CAGACACAAC GCAATCTACC CATTGCTCGC TTCCGGAAGG ACTTGCGCGA GGCACTGGAC 
ACGTCCCGCG TGGTCATCGT GGCCGGTGAC ACTGGCTGCG GCAAGTCCAC ACAGGTGCCC 
CAGTATCTCT ATGACTTTGG CTACCGCAGC ATTGCTTGCA CCCAGCCGCG CCGTTTGGCC 
TGCGTCTCCC TGTGCAAGCG CGTTGCGCAC GAGCTGCTGG ACGACTACGG CAGTCGGGTG 
GCATTCCAGA TCCGATTCGA GCGCAGTCGG ACGAAGCTCA CCAACATCCT TTTCATTACG 
GAGGGCTTGT TGCTGCGGCA ACTAGCGGTG GCCGCCAACC TAGACCAGTA CGATGCCCTG 
ATTTTGGACG AAATCCACGA GCGCAACTTA TTCGGTGACT TTCTACTGGG TGTTACCAAG 
TGCTTACTGA GGGCGCGGCC GCAACTGAAG CTGATACTCA TGTCTGCCAC CATCAACGTG 
GAGCTCTTCC ACGGCTACTT CGGCGAGGAG GGCGCACGGC TGGTCCAGGT GCCGGGACGT 
TTATTTCCCA TCAAACTGCG CTATCTGCCG CCTCCTGCAC TGGAACTAAA GGCGGGACAG 
GCGACATCCA AACGCTCTCA GAGAAACCGC ATCGATCCAG CTCCATTTGT CCAGGTGCTT 
AGCCTTATTG ACCAGCAGTA TCCGACCAGC GAGCGCGGTG ACGTCCTTAT CTTCGTTAGC 
GGCGTAAACG AAATCGAATC TGTTGTGGAG GCCGTTCACG AGTATGCCAC CGAACAGACT 
CATTGGCTCG TCCTGCCGCT GCACAGTGGC CAGGCGATCG CGGACCAGAG CAAGGTGTTT 
GACTACGCAC CCGAGGGCAT GCGCAAGTGC ATTGTGTCCA CCAACATAGC CGAAACGTCG 
CTCACCGTGG ACGGCGTGCG GTTCGTGGTC GACTCTGGCA AGGTCAAGGA GATGAACTTC 
GACGCAACCT GCAAGGGCCA GCGTCTGAAA GAGTTCTGGG TGTCCAAGTC GTCTGCGGAT 
CAGCGCAAGG GTCGTGCTGG TCGCACGGGA CCAGGTGTCT GTTTTCGGCT CTACACTGCG 
GAGCAATACA ACGCCTTCGA GGCATATCCC ACTCCGGAAA TTTACCGCGT GCCGCTCGAC 
ACGATGCTGC TGCAGATGGT GTCCATGGGA TTGCCCGACG TCCGGGCCTT TCCATTCATA 
GAGGCCCCGG AAACGGAGCG CATCGAGCAG ACTATTCTGG CGCTAAAGCA ACACTGCGCC 
CTCAGCGTGG AGGAAAAGAT CACACCGCTG GGCAGATCGC TGGCCAACTT GCCCGTAGAG 
CTGTCCATCG GCAAGATGCT GCTTATGGGC AGCGTGTTTC CCGAGGTGGA GCAGCTGCTG 
ACCCTGGCCG CCATGCTGAG CGTGCAGAAC CCGCTGACGA ATCGTGCCCA CACAGACCAG 
CGCTGCGTGC GCGAGCGGGA GTCTCTGGAG TCCGATCATG GCGACCTCTT CACCCTGGTG 
CGACTCTACA ACGTGTGGGT GCAGCTGAAG ATGCGGCGCG ATGGCACTCG ACAGTGGTGC 
CGTCGCTTGG GCATCGAGGA GCAGCGCTTC TATGAGGTGA CCAAGCTGCG TCAACAGTTC 
CAGCGCATCC TCGAGAGCTG CGGCATGGTG GTGGCCAGCG ATTCGGACTC CCAGTTGACC 
AGTGCCGAGC GCGCAACGCG ACATGGTGAG CTGCGCCAGC TGAAAGCCAT GAAGCGCCGC 
CAGCGATTTG AGCAACCCCG TCAGCGCAAG TTGCTCAAGC AGAGCGCTGG ACGGGTAGCG 
GAGGACGAGG AGGAGCAGGA GGAGGCGCAG GGCGACGATA TGAGGGACGT GGACTTCCGG 
CTGCGGTTCG ATCCTCGACA GTTGGCGCTG CTGGAGCGTT CGTCGCGGCT CGACCGACAC 
AGCGTCGTTG TGCTGCTAAA GCTGCTGCTC GGCAGCGGTT TCTATCCACA GCTGGCCATC 
AGCGACGAGT TCAACTACTG CAAGGGTGGT GGTCAGCAGT TCTTCCACAC GCGGCTCAAG 
CCCTTCGTGC TGCAGCACCC AAACTCGCAA TTCGCCAAGC ACTTCGAGCT GCTTAAGCTG 
ACCGAAAGCG ATCTGCTGCC CAAGCCAGAC TTCTACACGC CCAAGCTTCC GCTCAGCAAG 
CGCCACCAGT TGCTCTGTTA CCAGTCGCTG CTGGAGACCG CCAAGCCCTA TCTGATTAAC 
TGCATCCGCC TGCCGGCTGC CCAGACGCTA TTGCTCTTCA GCTTCGCCAT TGACACGAAC 
GCGGGCATCA CACAGATCGC CTGCGACGGC TGGCTGGGAC TGGATCTGCC CATGCCCGGC 
AGCGGTATGG AGCTTTTAAG ACGGGCCATC GAGTTGCGCC GACGTTGGAG CAGACTGCTT 
TACGACAAGC TGGATGACCT CAAAAGCAAA CAGGAATCAC CTTCCCATTC AAGTGACGAT 
AGGAGCAGCG CGCTGTGGCA AGATCTTGTG GACTATATGG CGCTGGATGT GGCGTATGCG 
ATACGCCGAC TGCTTCCCGC CGACATCAAG AGGCTCTACA CTCATCAGGC ACCTTCCGCG 
CGACTCGCGG AGCTCAAGGA GAACCCCTTT GCCGTCGACT TTCCCATGAC ACCGAACGAG 
GAAAAGGGCG GCCTCAACGT ATCGGAGCAC GTGGTGTACA GCTGCTTGGC CGAACAGCAG 
TGGACGACGG CCATGGATGC GACTCTACGG GCAGAGCCAT GGCAGTGTAC GCGCTGCGAT 
TTCGAGCTGG AGGAGTTCGA TGTGCTGGAG CAGCTGGTGC ACCGATCCAA ATGCAAGCGT 
CGCAAGGCGC AGGAAAGCCG CACGGCGAAA ACAACGAGCA CAGAGTCCTC AACGGAGGTA 
GCCAGTAGCT CCAGTTCGCA CTCCTCCGGC GGCTATTACT GCAACTCCTG CAAGAGGGAG 
TTGCGACTGA CAACGATCGA CATTCTGCGC CACAAAAAGC AGTGTCGAAA TAACAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728244.1
CDS	155..3574
Translation	MSRIKKKADEHASSRRRTQINLIEFSFLDHKAELVGLLQAKSRQVGRSLVENEADFWKFVGKYEAMMRSTGQPVLPPRLAEAELSSMQPYHRSKHLALRLDADELLQEARDQMQSQFRHILLVYLDFRQTEKFQRIRKLRQTQRNLPIARFRKDLREALDTSRVVIVAGDTGCGKSTQVPQYLYDFGYRSIACTQPRRLACVSLCKRVAHELLDDYGSRVAFQIRFERSRTKLTNILFITEGLLLRQLAVAANLDQYDALILDEIHERNLFGDFLLGVTKCLLRARPQLKLILMSATINVELFHGYFGEEGARLVQVPGRLFPIKLRYLPPPALELKAGQATSKRSQRNRIDPAPFVQVLSLIDQQYPTSERGDVLIFVSGVNEIESVVEAVHEYATEQTHWLVLPLHSGQAIADQSKVFDYAPEGMRKCIVSTNIAETSLTVDGVRFVVDSGKVKEMNFDATCKGQRLKEFWVSKSSADQRKGRAGRTGPGVCFRLYTAEQYNAFEAYPTPEIYRVPLDTMLLQMVSMGLPDVRAFPFIEAPETERIEQTILALKQHCALSVEEKITPLGRSLANLPVELSIGKMLLMGSVFPEVEQLLTLAAMLSVQNPLTNRAHTDQRCVRERESLESDHGDLFTLVRLYNVWVQLKMRRDGTRQWCRRLGIEEQRFYEVTKLRQQFQRILESCGMVVASDSDSQLTSAERATRHGELRQLKAMKRRQRFEQPRQRKLLKQSAGRVAEDEEEQEEAQGDDMRDVDFRLRFDPRQLALLERSSRLDRHSVVVLLKLLLGSGFYPQLAISDEFNYCKGGGQQFFHTRLKPFVLQHPNSQFAKHFELLKLTESDLLPKPDFYTPKLPLSKRHQLLCYQSLLETAKPYLINCIRLPAAQTLLLFSFAIDTNAGITQIACDGWLGLDLPMPGSGMELLRRAIELRRRWSRLLYDKLDDLKSKQESPSHSSDDRSSALWQDLVDYMALDVAYAIRRLLPADIKRLYTHQAPSARLAELKENPFAVDFPMTPNEEKGGLNVSEHVVYSCLAEQQWTTAMDATLRAEPWQCTRCDFELEEFDVLEQLVHRSKCKRRKAQESRTAKTTSTESSTEVASSSSSHSSGGYYCNSCKRELRLTTIDILRHKKQCRNNK

Target ORF information:

RefSeq Version	NM_167649.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG32533), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167649.2

ORF Insert Sequence:

ATGTCTCGGA TAAAGAAGAA GGCAGATGAA CATGCCTCCA GCCGCCGCAG GACTCAGATC 
AATCTGATCG AGTTCAGTTT CTTGGATCAC AAGGCGGAGC TCGTGGGCCT GCTGCAGGCC 
AAGAGCCGCC AGGTAGGCAG GAGTCTGGTG GAGAACGAGG CTGACTTCTG GAAGTTCGTG 
GGCAAGTACG AGGCTATGAT GCGCTCCACG GGCCAGCCCG TGCTGCCGCC TCGACTCGCG 
GAGGCGGAGC TGAGCAGCAT GCAGCCGTAT CACCGCAGCA AGCACTTGGC ATTGCGGCTG 
GACGCTGACG AACTGCTTCA GGAGGCGCGC GACCAAATGC AGTCCCAGTT TCGTCATATC 
TTGCTGGTGT ACTTAGACTT CCGCCAGACG GAAAAGTTCC AGCGTATCCG AAAGCTGCGG 
CAGACACAAC GCAATCTACC CATTGCTCGC TTCCGGAAGG ACTTGCGCGA GGCACTGGAC 
ACGTCCCGCG TGGTCATCGT GGCCGGTGAC ACTGGCTGCG GCAAGTCCAC ACAGGTGCCC 
CAGTATCTCT ATGACTTTGG CTACCGCAGC ATTGCTTGCA CCCAGCCGCG CCGTTTGGCC 
TGCGTCTCCC TGTGCAAGCG CGTTGCGCAC GAGCTGCTGG ACGACTACGG CAGTCGGGTG 
GCATTCCAGA TCCGATTCGA GCGCAGTCGG ACGAAGCTCA CCAACATCCT TTTCATTACG 
GAGGGCTTGT TGCTGCGGCA ACTAGCGGTG GCCGCCAACC TAGACCAGTA CGATGCCCTG 
ATTTTGGACG AAATCCACGA GCGCAACTTA TTCGGTGACT TTCTACTGGG TGTTACCAAG 
TGCTTACTGA GGGCGCGGCC GCAACTGAAG CTGATACTCA TGTCTGCCAC CATCAACGTG 
GAGCTCTTCC ACGGCTACTT CGGCGAGGAG GGCGCACGGC TGGTCCAGGT GCCGGGACGT 
TTATTTCCCA TCAAACTGCG CTATCTGCCG CCTCCTGCAC TGGAACTAAA GGCGGGACAG 
GCGACATCCA AACGCTCTCA GAGAAACCGC ATCGATCCAG CTCCATTTGT CCAGGTGCTT 
AGCCTTATTG ACCAGCAGTA TCCGACCAGC GAGCGCGGTG ACGTCCTTAT CTTCGTTAGC 
GGCGTAAACG AAATCGAATC TGTTGTGGAG GCCGTTCACG AGTATGCCAC CGAACAGACT 
CATTGGCTCG TCCTGCCGCT GCACAGTGGC CAGGCGATCG CGGACCAGAG CAAGGTGTTT 
GACTACGCAC CCGAGGGCAT GCGCAAGTGC ATTGTGTCCA CCAACATAGC CGAAACGTCG 
CTCACCGTGG ACGGCGTGCG GTTCGTGGTC GACTCTGGCA AGGTCAAGGA GATGAACTTC 
GACGCAACCT GCAAGGGCCA GCGTCTGAAA GAGTTCTGGG TGTCCAAGTC GTCTGCGGAT 
CAGCGCAAGG GTCGTGCTGG TCGCACGGGA CCAGGTGTCT GTTTTCGGCT CTACACTGCG 
GAGCAATACA ACGCCTTCGA GGCATATCCC ACTCCGGAAA TTTACCGCGT GCCGCTCGAC 
ACGATGCTGC TGCAGATGGT GTCCATGGGA TTGCCCGACG TCCGGGCCTT TCCATTCATA 
GAGGCCCCGG AAACGGAGCG CATCGAGCAG ACTATTCTGG CGCTAAAGCA ACACTGCGCC 
CTCAGCGTGG AGGAAAAGAT CACACCGCTG GGCAGATCGC TGGCCAACTT GCCCGTAGAG 
CTGTCCATCG GCAAGATGCT GCTTATGGGC AGCGTGTTTC CCGAGGTGGA GCAGCTGCTG 
ACCCTGGCCG CCATGCTGAG CGTGCAGAAC CCGCTGACGA ATCGTGCCCA CACAGACCAG 
CGCTGCGTGC GCGAGCGGGA GTCTCTGGAG TCCGATCATG GCGACCTCTT CACCCTGGTG 
CGACTCTACA ACGTGTGGGT GCAGCTGAAG ATGCGGCGCG ATGGCACTCG ACAGTGGTGC 
CGTCGCTTGG GCATCGAGGA GCAGCGCTTC TATGAGGTGA CCAAGCTGCG TCAACAGTTC 
CAGCGCATCC TCGAGAGCTG CGGCATGGTG GTGGCCAGCG ATTCGGACTC CCAGTTGACC 
AGTGCCGAGC GCGCAACGCG ACATGGTGAG CTGCGCCAGC TGAAAGCCAT GAAGCGCCGC 
CAGCGATTTG AGCAACCCCG TCAGCGCAAG TTGCTCAAGC AGAGCGCTGG ACGGGTAGCG 
GAGGACGAGG AGGAGCAGGA GGAGGCGCAG GGCGACGATA TGAGGGACGT GGACTTCCGG 
CTGCGGTTCG ATCCTCGACA GTTGGCGCTG CTGGAGCGTT CGTCGCGGCT CGACCGACAC 
AGCGTCGTTG TGCTGCTAAA GCTGCTGCTC GGCAGCGGTT TCTATCCACA GCTGGCCATC 
AGCGACGAGT TCAACTACTG CAAGGGTGGT GGTCAGCAGT TCTTCCACAC GCGGCTCAAG 
CCCTTCGTGC TGCAGCACCC AAACTCGCAA TTCGCCAAGC ACTTCGAGCT GCTTAAGCTG 
ACCGAAAGCG ATCTGCTGCC CAAGCCAGAC TTCTACACGC CCAAGCTTCC GCTCAGCAAG 
CGCCACCAGT TGCTCTGTTA CCAGTCGCTG CTGGAGACCG CCAAGCCCTA TCTGATTAAC 
TGCATCCGCC TGCCGGCTGC CCAGACGCTA TTGCTCTTCA GCTTCGCCAT TGACACGAAC 
GCGGGCATCA CACAGATCGC CTGCGACGGC TGGCTGGGAC TGGATCTGCC CATGCCCGGC 
AGCGGTATGG AGCTTTTAAG ACGGGCCATC GAGTTGCGCC GACGTTGGAG CAGACTGCTT 
TACGACAAGC TGGATGACCT CAAAAGCAAA CAGGAATCAC CTTCCCATTC AAGTGACGAT 
AGGAGCAGCG CGCTGTGGCA AGATCTTGTG GACTATATGG CGCTGGATGT GGCGTATGCG 
ATACGCCGAC TGCTTCCCGC CGACATCAAG AGGCTCTACA CTCATCAGGC ACCTTCCGCG 
CGACTCGCGG AGCTCAAGGA GAACCCCTTT GCCGTCGACT TTCCCATGAC ACCGAACGAG 
GAAAAGGGCG GCCTCAACGT ATCGGAGCAC GTGGTGTACA GCTGCTTGGC CGAACAGCAG 
TGGACGACGG CCATGGATGC GACTCTACGG GCAGAGCCAT GGCAGTGTAC GCGCTGCGAT 
TTCGAGCTGG AGGAGTTCGA TGTGCTGGAG CAGCTGGTGC ACCGATCCAA ATGCAAGCGT 
CGCAAGGCGC AGGAAAGCCG CACGGCGAAA ACAACGAGCA CAGAGTCCTC AACGGAGGTA 
GCCAGTAGCT CCAGTTCGCA CTCCTCCGGC GGCTATTACT GCAACTCCTG CAAGAGGGAG 
TTGCGACTGA CAACGATCGA CATTCTGCGC CACAAAAAGC AGTGTCGAAA TAACAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG32533 ( NM_001298508.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285437.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG32533), mRNA.

Price & Availability↑

CloneID	OFa02118
Clone ID Related Accession (Same CDS sequence)	NM_167649.2 , NM_001298508.1
Accession Version	NM_001298508.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTCGGA TAAAGAAGAA GGCAGATGAA CATGCCTCCA GCCGCCGCAG GACTCAGATC 
AATCTGATCG AGTTCAGTTT CTTGGATCAC AAGGCGGAGC TCGTGGGCCT GCTGCAGGCC 
AAGAGCCGCC AGGTAGGCAG GAGTCTGGTG GAGAACGAGG CTGACTTCTG GAAGTTCGTG 
GGCAAGTACG AGGCTATGAT GCGCTCCACG GGCCAGCCCG TGCTGCCGCC TCGACTCGCG 
GAGGCGGAGC TGAGCAGCAT GCAGCCGTAT CACCGCAGCA AGCACTTGGC ATTGCGGCTG 
GACGCTGACG AACTGCTTCA GGAGGCGCGC GACCAAATGC AGTCCCAGTT TCGTCATATC 
TTGCTGGTGT ACTTAGACTT CCGCCAGACG GAAAAGTTCC AGCGTATCCG AAAGCTGCGG 
CAGACACAAC GCAATCTACC CATTGCTCGC TTCCGGAAGG ACTTGCGCGA GGCACTGGAC 
ACGTCCCGCG TGGTCATCGT GGCCGGTGAC ACTGGCTGCG GCAAGTCCAC ACAGGTGCCC 
CAGTATCTCT ATGACTTTGG CTACCGCAGC ATTGCTTGCA CCCAGCCGCG CCGTTTGGCC 
TGCGTCTCCC TGTGCAAGCG CGTTGCGCAC GAGCTGCTGG ACGACTACGG CAGTCGGGTG 
GCATTCCAGA TCCGATTCGA GCGCAGTCGG ACGAAGCTCA CCAACATCCT TTTCATTACG 
GAGGGCTTGT TGCTGCGGCA ACTAGCGGTG GCCGCCAACC TAGACCAGTA CGATGCCCTG 
ATTTTGGACG AAATCCACGA GCGCAACTTA TTCGGTGACT TTCTACTGGG TGTTACCAAG 
TGCTTACTGA GGGCGCGGCC GCAACTGAAG CTGATACTCA TGTCTGCCAC CATCAACGTG 
GAGCTCTTCC ACGGCTACTT CGGCGAGGAG GGCGCACGGC TGGTCCAGGT GCCGGGACGT 
TTATTTCCCA TCAAACTGCG CTATCTGCCG CCTCCTGCAC TGGAACTAAA GGCGGGACAG 
GCGACATCCA AACGCTCTCA GAGAAACCGC ATCGATCCAG CTCCATTTGT CCAGGTGCTT 
AGCCTTATTG ACCAGCAGTA TCCGACCAGC GAGCGCGGTG ACGTCCTTAT CTTCGTTAGC 
GGCGTAAACG AAATCGAATC TGTTGTGGAG GCCGTTCACG AGTATGCCAC CGAACAGACT 
CATTGGCTCG TCCTGCCGCT GCACAGTGGC CAGGCGATCG CGGACCAGAG CAAGGTGTTT 
GACTACGCAC CCGAGGGCAT GCGCAAGTGC ATTGTGTCCA CCAACATAGC CGAAACGTCG 
CTCACCGTGG ACGGCGTGCG GTTCGTGGTC GACTCTGGCA AGGTCAAGGA GATGAACTTC 
GACGCAACCT GCAAGGGCCA GCGTCTGAAA GAGTTCTGGG TGTCCAAGTC GTCTGCGGAT 
CAGCGCAAGG GTCGTGCTGG TCGCACGGGA CCAGGTGTCT GTTTTCGGCT CTACACTGCG 
GAGCAATACA ACGCCTTCGA GGCATATCCC ACTCCGGAAA TTTACCGCGT GCCGCTCGAC 
ACGATGCTGC TGCAGATGGT GTCCATGGGA TTGCCCGACG TCCGGGCCTT TCCATTCATA 
GAGGCCCCGG AAACGGAGCG CATCGAGCAG ACTATTCTGG CGCTAAAGCA ACACTGCGCC 
CTCAGCGTGG AGGAAAAGAT CACACCGCTG GGCAGATCGC TGGCCAACTT GCCCGTAGAG 
CTGTCCATCG GCAAGATGCT GCTTATGGGC AGCGTGTTTC CCGAGGTGGA GCAGCTGCTG 
ACCCTGGCCG CCATGCTGAG CGTGCAGAAC CCGCTGACGA ATCGTGCCCA CACAGACCAG 
CGCTGCGTGC GCGAGCGGGA GTCTCTGGAG TCCGATCATG GCGACCTCTT CACCCTGGTG 
CGACTCTACA ACGTGTGGGT GCAGCTGAAG ATGCGGCGCG ATGGCACTCG ACAGTGGTGC 
CGTCGCTTGG GCATCGAGGA GCAGCGCTTC TATGAGGTGA CCAAGCTGCG TCAACAGTTC 
CAGCGCATCC TCGAGAGCTG CGGCATGGTG GTGGCCAGCG ATTCGGACTC CCAGTTGACC 
AGTGCCGAGC GCGCAACGCG ACATGGTGAG CTGCGCCAGC TGAAAGCCAT GAAGCGCCGC 
CAGCGATTTG AGCAACCCCG TCAGCGCAAG TTGCTCAAGC AGAGCGCTGG ACGGGTAGCG 
GAGGACGAGG AGGAGCAGGA GGAGGCGCAG GGCGACGATA TGAGGGACGT GGACTTCCGG 
CTGCGGTTCG ATCCTCGACA GTTGGCGCTG CTGGAGCGTT CGTCGCGGCT CGACCGACAC 
AGCGTCGTTG TGCTGCTAAA GCTGCTGCTC GGCAGCGGTT TCTATCCACA GCTGGCCATC 
AGCGACGAGT TCAACTACTG CAAGGGTGGT GGTCAGCAGT TCTTCCACAC GCGGCTCAAG 
CCCTTCGTGC TGCAGCACCC AAACTCGCAA TTCGCCAAGC ACTTCGAGCT GCTTAAGCTG 
ACCGAAAGCG ATCTGCTGCC CAAGCCAGAC TTCTACACGC CCAAGCTTCC GCTCAGCAAG 
CGCCACCAGT TGCTCTGTTA CCAGTCGCTG CTGGAGACCG CCAAGCCCTA TCTGATTAAC 
TGCATCCGCC TGCCGGCTGC CCAGACGCTA TTGCTCTTCA GCTTCGCCAT TGACACGAAC 
GCGGGCATCA CACAGATCGC CTGCGACGGC TGGCTGGGAC TGGATCTGCC CATGCCCGGC 
AGCGGTATGG AGCTTTTAAG ACGGGCCATC GAGTTGCGCC GACGTTGGAG CAGACTGCTT 
TACGACAAGC TGGATGACCT CAAAAGCAAA CAGGAATCAC CTTCCCATTC AAGTGACGAT 
AGGAGCAGCG CGCTGTGGCA AGATCTTGTG GACTATATGG CGCTGGATGT GGCGTATGCG 
ATACGCCGAC TGCTTCCCGC CGACATCAAG AGGCTCTACA CTCATCAGGC ACCTTCCGCG 
CGACTCGCGG AGCTCAAGGA GAACCCCTTT GCCGTCGACT TTCCCATGAC ACCGAACGAG 
GAAAAGGGCG GCCTCAACGT ATCGGAGCAC GTGGTGTACA GCTGCTTGGC CGAACAGCAG 
TGGACGACGG CCATGGATGC GACTCTACGG GCAGAGCCAT GGCAGTGTAC GCGCTGCGAT 
TTCGAGCTGG AGGAGTTCGA TGTGCTGGAG CAGCTGGTGC ACCGATCCAA ATGCAAGCGT 
CGCAAGGCGC AGGAAAGCCG CACGGCGAAA ACAACGAGCA CAGAGTCCTC AACGGAGGTA 
GCCAGTAGCT CCAGTTCGCA CTCCTCCGGC GGCTATTACT GCAACTCCTG CAAGAGGGAG 
TTGCGACTGA CAACGATCGA CATTCTGCGC CACAAAAAGC AGTGTCGAAA TAACAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285437.1
CDS	63..3482
Translation	MSRIKKKADEHASSRRRTQINLIEFSFLDHKAELVGLLQAKSRQVGRSLVENEADFWKFVGKYEAMMRSTGQPVLPPRLAEAELSSMQPYHRSKHLALRLDADELLQEARDQMQSQFRHILLVYLDFRQTEKFQRIRKLRQTQRNLPIARFRKDLREALDTSRVVIVAGDTGCGKSTQVPQYLYDFGYRSIACTQPRRLACVSLCKRVAHELLDDYGSRVAFQIRFERSRTKLTNILFITEGLLLRQLAVAANLDQYDALILDEIHERNLFGDFLLGVTKCLLRARPQLKLILMSATINVELFHGYFGEEGARLVQVPGRLFPIKLRYLPPPALELKAGQATSKRSQRNRIDPAPFVQVLSLIDQQYPTSERGDVLIFVSGVNEIESVVEAVHEYATEQTHWLVLPLHSGQAIADQSKVFDYAPEGMRKCIVSTNIAETSLTVDGVRFVVDSGKVKEMNFDATCKGQRLKEFWVSKSSADQRKGRAGRTGPGVCFRLYTAEQYNAFEAYPTPEIYRVPLDTMLLQMVSMGLPDVRAFPFIEAPETERIEQTILALKQHCALSVEEKITPLGRSLANLPVELSIGKMLLMGSVFPEVEQLLTLAAMLSVQNPLTNRAHTDQRCVRERESLESDHGDLFTLVRLYNVWVQLKMRRDGTRQWCRRLGIEEQRFYEVTKLRQQFQRILESCGMVVASDSDSQLTSAERATRHGELRQLKAMKRRQRFEQPRQRKLLKQSAGRVAEDEEEQEEAQGDDMRDVDFRLRFDPRQLALLERSSRLDRHSVVVLLKLLLGSGFYPQLAISDEFNYCKGGGQQFFHTRLKPFVLQHPNSQFAKHFELLKLTESDLLPKPDFYTPKLPLSKRHQLLCYQSLLETAKPYLINCIRLPAAQTLLLFSFAIDTNAGITQIACDGWLGLDLPMPGSGMELLRRAIELRRRWSRLLYDKLDDLKSKQESPSHSSDDRSSALWQDLVDYMALDVAYAIRRLLPADIKRLYTHQAPSARLAELKENPFAVDFPMTPNEEKGGLNVSEHVVYSCLAEQQWTTAMDATLRAEPWQCTRCDFELEEFDVLEQLVHRSKCKRRKAQESRTAKTTSTESSTEVASSSSSHSSGGYYCNSCKRELRLTTIDILRHKKQCRNNK

Target ORF information:

RefSeq Version	NM_001298508.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG32533), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298508.1

ORF Insert Sequence:

ATGTCTCGGA TAAAGAAGAA GGCAGATGAA CATGCCTCCA GCCGCCGCAG GACTCAGATC 
AATCTGATCG AGTTCAGTTT CTTGGATCAC AAGGCGGAGC TCGTGGGCCT GCTGCAGGCC 
AAGAGCCGCC AGGTAGGCAG GAGTCTGGTG GAGAACGAGG CTGACTTCTG GAAGTTCGTG 
GGCAAGTACG AGGCTATGAT GCGCTCCACG GGCCAGCCCG TGCTGCCGCC TCGACTCGCG 
GAGGCGGAGC TGAGCAGCAT GCAGCCGTAT CACCGCAGCA AGCACTTGGC ATTGCGGCTG 
GACGCTGACG AACTGCTTCA GGAGGCGCGC GACCAAATGC AGTCCCAGTT TCGTCATATC 
TTGCTGGTGT ACTTAGACTT CCGCCAGACG GAAAAGTTCC AGCGTATCCG AAAGCTGCGG 
CAGACACAAC GCAATCTACC CATTGCTCGC TTCCGGAAGG ACTTGCGCGA GGCACTGGAC 
ACGTCCCGCG TGGTCATCGT GGCCGGTGAC ACTGGCTGCG GCAAGTCCAC ACAGGTGCCC 
CAGTATCTCT ATGACTTTGG CTACCGCAGC ATTGCTTGCA CCCAGCCGCG CCGTTTGGCC 
TGCGTCTCCC TGTGCAAGCG CGTTGCGCAC GAGCTGCTGG ACGACTACGG CAGTCGGGTG 
GCATTCCAGA TCCGATTCGA GCGCAGTCGG ACGAAGCTCA CCAACATCCT TTTCATTACG 
GAGGGCTTGT TGCTGCGGCA ACTAGCGGTG GCCGCCAACC TAGACCAGTA CGATGCCCTG 
ATTTTGGACG AAATCCACGA GCGCAACTTA TTCGGTGACT TTCTACTGGG TGTTACCAAG 
TGCTTACTGA GGGCGCGGCC GCAACTGAAG CTGATACTCA TGTCTGCCAC CATCAACGTG 
GAGCTCTTCC ACGGCTACTT CGGCGAGGAG GGCGCACGGC TGGTCCAGGT GCCGGGACGT 
TTATTTCCCA TCAAACTGCG CTATCTGCCG CCTCCTGCAC TGGAACTAAA GGCGGGACAG 
GCGACATCCA AACGCTCTCA GAGAAACCGC ATCGATCCAG CTCCATTTGT CCAGGTGCTT 
AGCCTTATTG ACCAGCAGTA TCCGACCAGC GAGCGCGGTG ACGTCCTTAT CTTCGTTAGC 
GGCGTAAACG AAATCGAATC TGTTGTGGAG GCCGTTCACG AGTATGCCAC CGAACAGACT 
CATTGGCTCG TCCTGCCGCT GCACAGTGGC CAGGCGATCG CGGACCAGAG CAAGGTGTTT 
GACTACGCAC CCGAGGGCAT GCGCAAGTGC ATTGTGTCCA CCAACATAGC CGAAACGTCG 
CTCACCGTGG ACGGCGTGCG GTTCGTGGTC GACTCTGGCA AGGTCAAGGA GATGAACTTC 
GACGCAACCT GCAAGGGCCA GCGTCTGAAA GAGTTCTGGG TGTCCAAGTC GTCTGCGGAT 
CAGCGCAAGG GTCGTGCTGG TCGCACGGGA CCAGGTGTCT GTTTTCGGCT CTACACTGCG 
GAGCAATACA ACGCCTTCGA GGCATATCCC ACTCCGGAAA TTTACCGCGT GCCGCTCGAC 
ACGATGCTGC TGCAGATGGT GTCCATGGGA TTGCCCGACG TCCGGGCCTT TCCATTCATA 
GAGGCCCCGG AAACGGAGCG CATCGAGCAG ACTATTCTGG CGCTAAAGCA ACACTGCGCC 
CTCAGCGTGG AGGAAAAGAT CACACCGCTG GGCAGATCGC TGGCCAACTT GCCCGTAGAG 
CTGTCCATCG GCAAGATGCT GCTTATGGGC AGCGTGTTTC CCGAGGTGGA GCAGCTGCTG 
ACCCTGGCCG CCATGCTGAG CGTGCAGAAC CCGCTGACGA ATCGTGCCCA CACAGACCAG 
CGCTGCGTGC GCGAGCGGGA GTCTCTGGAG TCCGATCATG GCGACCTCTT CACCCTGGTG 
CGACTCTACA ACGTGTGGGT GCAGCTGAAG ATGCGGCGCG ATGGCACTCG ACAGTGGTGC 
CGTCGCTTGG GCATCGAGGA GCAGCGCTTC TATGAGGTGA CCAAGCTGCG TCAACAGTTC 
CAGCGCATCC TCGAGAGCTG CGGCATGGTG GTGGCCAGCG ATTCGGACTC CCAGTTGACC 
AGTGCCGAGC GCGCAACGCG ACATGGTGAG CTGCGCCAGC TGAAAGCCAT GAAGCGCCGC 
CAGCGATTTG AGCAACCCCG TCAGCGCAAG TTGCTCAAGC AGAGCGCTGG ACGGGTAGCG 
GAGGACGAGG AGGAGCAGGA GGAGGCGCAG GGCGACGATA TGAGGGACGT GGACTTCCGG 
CTGCGGTTCG ATCCTCGACA GTTGGCGCTG CTGGAGCGTT CGTCGCGGCT CGACCGACAC 
AGCGTCGTTG TGCTGCTAAA GCTGCTGCTC GGCAGCGGTT TCTATCCACA GCTGGCCATC 
AGCGACGAGT TCAACTACTG CAAGGGTGGT GGTCAGCAGT TCTTCCACAC GCGGCTCAAG 
CCCTTCGTGC TGCAGCACCC AAACTCGCAA TTCGCCAAGC ACTTCGAGCT GCTTAAGCTG 
ACCGAAAGCG ATCTGCTGCC CAAGCCAGAC TTCTACACGC CCAAGCTTCC GCTCAGCAAG 
CGCCACCAGT TGCTCTGTTA CCAGTCGCTG CTGGAGACCG CCAAGCCCTA TCTGATTAAC 
TGCATCCGCC TGCCGGCTGC CCAGACGCTA TTGCTCTTCA GCTTCGCCAT TGACACGAAC 
GCGGGCATCA CACAGATCGC CTGCGACGGC TGGCTGGGAC TGGATCTGCC CATGCCCGGC 
AGCGGTATGG AGCTTTTAAG ACGGGCCATC GAGTTGCGCC GACGTTGGAG CAGACTGCTT 
TACGACAAGC TGGATGACCT CAAAAGCAAA CAGGAATCAC CTTCCCATTC AAGTGACGAT 
AGGAGCAGCG CGCTGTGGCA AGATCTTGTG GACTATATGG CGCTGGATGT GGCGTATGCG 
ATACGCCGAC TGCTTCCCGC CGACATCAAG AGGCTCTACA CTCATCAGGC ACCTTCCGCG 
CGACTCGCGG AGCTCAAGGA GAACCCCTTT GCCGTCGACT TTCCCATGAC ACCGAACGAG 
GAAAAGGGCG GCCTCAACGT ATCGGAGCAC GTGGTGTACA GCTGCTTGGC CGAACAGCAG 
TGGACGACGG CCATGGATGC GACTCTACGG GCAGAGCCAT GGCAGTGTAC GCGCTGCGAT 
TTCGAGCTGG AGGAGTTCGA TGTGCTGGAG CAGCTGGTGC ACCGATCCAA ATGCAAGCGT 
CGCAAGGCGC AGGAAAGCCG CACGGCGAAA ACAACGAGCA CAGAGTCCTC AACGGAGGTA 
GCCAGTAGCT CCAGTTCGCA CTCCTCCGGC GGCTATTACT GCAACTCCTG CAAGAGGGAG 
TTGCGACTGA CAACGATCGA CATTCTGCGC CACAAAAAGC AGTGTCGAAA TAACAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG32533 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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