Arp2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Arp2
Entrez Gene ID	32623
Full Name	Actin-related protein 2
Synonyms	ARP14D,ARP2,Actr14D,Arp14D,CG9901,Dmel\CG9901,arp2
General protein information	Preferred Names Actin-related protein 2 Names CG9901 gene product from transcript CG9901-RB Actin-related protein 14 Da Actin-related protein 14D Arp14D Arp2-PA Arp2-PB Arp2-PC CG9901-PA CG9901-PB CG9901-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 14E1-14E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001272692.1	NP_001259621.1	Actin-related protein 2, isoform C
NM_001103531.2	NP_001097001.1	Actin-related protein 2, isoform B
NM_078648.4	NP_523372.2	Actin-related protein 2, isoform A

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ACTR2	XP_852707.1
Danio rerio (zebrafish)	actr2b	NP_001017542.1
Pan troglodytes (chimpanzee)	ACTR2	XP_001155450.3
	ACTR2	XP_001090768.2
Rattus norvegicus (Norway rat)	Actr2	NP_001009268.1
Gallus gallus (chicken)	ACTR2	NP_990555.1
Danio rerio (zebrafish)	actr2a	NP_998664.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000985	XP_309181.5
Saccharomyces cerevisiae (baker's yeast)	ARP2	NP_010255.1
Arabidopsis thaliana (thale cress)	ARP2	NP_189336.1
Oryza sativa (rice)	Os08g0369300	NP_001061655.2
Drosophila melanogaster (fruit fly)	Arp2	NP_001259621.1
Neurospora crassa	NCU07171	XP_957396.1
Xenopus tropicalis (tropical clawed frog)	actr2	NP_001025569.1
Bos taurus (cattle)	ACTR2	NP_001095683.1
Eremothecium gossypii	AGOS_ADR316W	NP_984412.1
Schizosaccharomyces pombe (fission yeast)	arp2	XP_001713111.1
Magnaporthe oryzae (rice blast fungus)	MGG_17001	XP_003715887.1
Homo sapiens (human)	ACTR2	NP_001005386.1
Mus musculus (house mouse)	Actr2	NP_666355.1
Caenorhabditis elegans (roundworm)	arx-2	NP_505657.1
Kluyveromyces lactis	KLLA0C03850g	XP_452368.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Arp2/3 has an essential role in the process of secretory cargo expulsion and integration of vesicular membranes with the apical plasma membrane.
Title: Arp2/3-mediated F-actin formation controls regulated exocytosis in vivo.

Cofilin promotes phosphate dissociation and severs filaments, generating new pointed ends and rendering Arp2/3-generated networks competent to bind tropomyosin
Title: Arp2/3 complex and cofilin modulate binding of tropomyosin to branched actin networks.

Arp2/3 has a role in PI(4,5)P2 regulation of myoblast fusion in Drosophila
Title: PI(4,5)P2 regulates myoblast fusion through Arp2/3 regulator localization at the fusion site.

Abp1 and Arp2/3 complex-mediated actin cytoskeletal dynamics drive both synaptic bouton formation and neuromuscular junction branching.
Title: Terminal axonal arborization and synaptic bouton formation critically rely on abp1 and the arp2/3 complex.

The data indicate that WASp-dependent Arp2/3 actin polymerization is crucial for apical presentation of Delta, providing a mechanistic link between actin polymerization and Notch signalling.
Title: The Arp2/3 complex and WASp are required for apical trafficking of Delta into microvilli during cell fate specification of sensory organ precursors.

The following Arp2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Arp2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01828	NM_001272692.1 Latest version!	Drosophila melanogaster Actin-related protein 2 (Arp2), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa03033	NM_078648.4 Latest version!	Drosophila melanogaster Actin-related protein 2 (Arp2), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OFa03033	NM_001103531.2 Latest version!	Drosophila melanogaster Actin-related protein 2 (Arp2), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Arp2 ( NM_001272692.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259621.1 Drosophila melanogaster Actin-related protein 2 (Arp2), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa01828
Clone ID Related Accession (Same CDS sequence)	NM_001272692.1
Accession Version	NM_001272692.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1200bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Actin-related protein 2, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACAGCA AGGGTCGAAA TGTTATCGTC TGTGATAACG GCACTGGGTT CGTTAAGTGC 
GGATATGCCG GCAGCAATTT CCCCACCCAC ATTTTTCCCT CGATGGTGGG ACGACCCATG 
ATTCGGGCGG TCAACAAAAT CGGCGATATC GAAGTCAAGG ATTTACATGT CGATGATCTG 
ATGGTCGGCG ATGAGGCCTC ACAGCTGCGA TCCCTGCTGG AGGTCTCCTA TCCGATGGAG 
AACGGTGTGG TGCGCAACTG GGACGACATG TGCCACGTGT GGGACTACAC GTTCGGTCCC 
AAAAAGATGG ACATCGATCC GACGAACACA AAAATCCTGC TAACCGAGCC GCCCATGAAT 
CCCACAAAGA ATCGCGAGAA GATGATTGAG GTGATGTTCG AGAAATATGG CTTCGATTCG 
GCGTACATTG CCATCCAGGC CGTGCTGACG CTATATGCCC AGGGTCTGAT CTCTGGCGTG 
GTGATCGATT CGGGCGATGG TGTAACGCAT ATATGTCCCG TCTACGAGGA GTTTGCCCTG 
CCCCATCTGA CGCGACGATT GGACATTGCC GGTCGGGATA TCACTCGCTA TTTGATTAAG 
TTACTGTTAC TACGTGGCTA CGCTTTCAAT CATTCCGCCG ACTTCGAAAC GGTTCGCATA 
ATGAAGGAGA AGCTCTGCTA CATTGGCTAC GACATCGAGA TGGAGCAGCG TTTGGCCCTG 
GAGACGACGG TGCTGGTGGA ATCGTACACG CTACCCGACG GTCGTGTTAT CAAGGTCGGT 
GGCGAGAGAT TTGAGGCGCC AGAGGCTCTG TTCCAGCCGC ATTTGATCAA CGTCGAGGGA 
CCGGGCATTG CGGAACTGGC CTTCAACACC ATCCAGGCGG CAGACATCGA CATACGGCCC 
GAACTCTACA AGCACATAGT TCTGTCCGGC GGCTCCACCA TGTATCCGGG TCTTCCCAGT 
CGGCTGGAGC GTGAGATCAA ACAGCTGTAC CTGGAGCGGG TACTCAAGAA CGATACGGAA 
AAGCTGGCGA AATTCAAAAT ACGCATTGAG GATCCACCGC GACGCAAGGA TATGGTCTTT 
ATCGGCGGAG CTGTTCTGGC GGAGGTGACA AAGGATCGCG ACGGCTTCTG GATGTCCAAG 
CAGGAGTATC AGGAACAGGG TCTCAAAGTA TTGCAAAAGC TGCAAAAGAT CAGTCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259621.1
CDS	561..1760
Translation	MDSKGRNVIVCDNGTGFVKCGYAGSNFPTHIFPSMVGRPMIRAVNKIGDIEVKDLHVDDLMVGDEASQLRSLLEVSYPMENGVVRNWDDMCHVWDYTFGPKKMDIDPTNTKILLTEPPMNPTKNREKMIEVMFEKYGFDSAYIAIQAVLTLYAQGLISGVVIDSGDGVTHICPVYEEFALPHLTRRLDIAGRDITRYLIKLLLLRGYAFNHSADFETVRIMKEKLCYIGYDIEMEQRLALETTVLVESYTLPDGRVIKVGGERFEAPEALFQPHLINVEGPGIAELAFNTIQAADIDIRPELYKHIVLSGGSTMYPGLPSRLEREIKQLYLERVLKNDTEKLAKFKIRIEDPPRRKDMVFIGGAVLAEVTKDRDGFWMSKQEYQEQGLKVLQKLQKISH

Target ORF information:

RefSeq Version	NM_001272692.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Actin-related protein 2 (Arp2), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272692.1

ORF Insert Sequence:

ATGGACAGCA AGGGTCGAAA TGTTATCGTC TGTGATAACG GCACTGGGTT CGTTAAGTGC 
GGATATGCCG GCAGCAATTT CCCCACCCAC ATTTTTCCCT CGATGGTGGG ACGACCCATG 
ATTCGGGCGG TCAACAAAAT CGGCGATATC GAAGTCAAGG ATTTACATGT CGATGATCTG 
ATGGTCGGCG ATGAGGCCTC ACAGCTGCGA TCCCTGCTGG AGGTCTCCTA TCCGATGGAG 
AACGGTGTGG TGCGCAACTG GGACGACATG TGCCACGTGT GGGACTACAC GTTCGGTCCC 
AAAAAGATGG ACATCGATCC GACGAACACA AAAATCCTGC TAACCGAGCC GCCCATGAAT 
CCCACAAAGA ATCGCGAGAA GATGATTGAG GTGATGTTCG AGAAATATGG CTTCGATTCG 
GCGTACATTG CCATCCAGGC CGTGCTGACG CTATATGCCC AGGGTCTGAT CTCTGGCGTG 
GTGATCGATT CGGGCGATGG TGTAACGCAT ATATGTCCCG TCTACGAGGA GTTTGCCCTG 
CCCCATCTGA CGCGACGATT GGACATTGCC GGTCGGGATA TCACTCGCTA TTTGATTAAG 
TTACTGTTAC TACGTGGCTA CGCTTTCAAT CATTCCGCCG ACTTCGAAAC GGTTCGCATA 
ATGAAGGAGA AGCTCTGCTA CATTGGCTAC GACATCGAGA TGGAGCAGCG TTTGGCCCTG 
GAGACGACGG TGCTGGTGGA ATCGTACACG CTACCCGACG GTCGTGTTAT CAAGGTCGGT 
GGCGAGAGAT TTGAGGCGCC AGAGGCTCTG TTCCAGCCGC ATTTGATCAA CGTCGAGGGA 
CCGGGCATTG CGGAACTGGC CTTCAACACC ATCCAGGCGG CAGACATCGA CATACGGCCC 
GAACTCTACA AGCACATAGT TCTGTCCGGC GGCTCCACCA TGTATCCGGG TCTTCCCAGT 
CGGCTGGAGC GTGAGATCAA ACAGCTGTAC CTGGAGCGGG TACTCAAGAA CGATACGGAA 
AAGCTGGCGA AATTCAAAAT ACGCATTGAG GATCCACCGC GACGCAAGGA TATGGTCTTT 
ATCGGCGGAG CTGTTCTGGC GGAGGTGACA AAGGATCGCG ACGGCTTCTG GATGTCCAAG 
CAGGAGTATC AGGAACAGGG TCTCAAAGTA TTGCAAAAGC TGCAAAAGAT CAGTCACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arp2 ( NM_001103531.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097001.1 Drosophila melanogaster Actin-related protein 2 (Arp2), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa03033
Clone ID Related Accession (Same CDS sequence)	NM_001103531.2 , NM_078648.4
Accession Version	NM_001103531.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Actin-related protein 2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_001103531.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACAGCA AGGGTCGAAA TGTTATCGTC TGTGATAACG GCACTGGGTT CGTTAAGTGC 
GGATATGCCG GCAGCAATTT CCCCACCCAC ATTTTTCCCT CGATGGTGGG ACGACCCATG 
ATTCGGGCGG TCAACAAAAT CGGCGATATC GAAGTCAAGG ATCTGATGGT CGGCGATGAG 
GCCTCACAGC TGCGATCCCT GCTGGAGGTC TCCTATCCGA TGGAGAACGG TGTGGTGCGC 
AACTGGGACG ACATGTGCCA CGTGTGGGAC TACACGTTCG GTCCCAAAAA GATGGACATC 
GATCCGACGA ACACAAAAAT CCTGCTAACC GAGCCGCCCA TGAATCCCAC AAAGAATCGC 
GAGAAGATGA TTGAGGTGAT GTTCGAGAAA TATGGCTTCG ATTCGGCGTA CATTGCCATC 
CAGGCCGTGC TGACGCTATA TGCCCAGGGT CTGATCTCTG GCGTGGTGAT CGATTCGGGC 
GATGGTGTAA CGCATATATG TCCCGTCTAC GAGGAGTTTG CCCTGCCCCA TCTGACGCGA 
CGATTGGACA TTGCCGGTCG GGATATCACT CGCTATTTGA TTAAGTTACT GTTACTACGT 
GGCTACGCTT TCAATCATTC CGCCGACTTC GAAACGGTTC GCATAATGAA GGAGAAGCTC 
TGCTACATTG GCTACGACAT CGAGATGGAG CAGCGTTTGG CCCTGGAGAC GACGGTGCTG 
GTGGAATCGT ACACGCTACC CGACGGTCGT GTTATCAAGG TCGGTGGCGA GAGATTTGAG 
GCGCCAGAGG CTCTGTTCCA GCCGCATTTG ATCAACGTCG AGGGACCGGG CATTGCGGAA 
CTGGCCTTCA ACACCATCCA GGCGGCAGAC ATCGACATAC GGCCCGAACT CTACAAGCAC 
ATAGTTCTGT CCGGCGGCTC CACCATGTAT CCGGGTCTTC CCAGTCGGCT GGAGCGTGAG 
ATCAAACAGC TGTACCTGGA GCGGGTACTC AAGAACGATA CGGAAAAGCT GGCGAAATTC 
AAAATACGCA TTGAGGATCC ACCGCGACGC AAGGATATGG TCTTTATCGG CGGAGCTGTT 
CTGGCGGAGG TGACAAAGGA TCGCGACGGC TTCTGGATGT CCAAGCAGGA GTATCAGGAA 
CAGGGTCTCA AAGTATTGCA AAAGCTGCAA AAGATCAGTC ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097001.1
CDS	339..1523
Translation	MDSKGRNVIVCDNGTGFVKCGYAGSNFPTHIFPSMVGRPMIRAVNKIGDIEVKDLMVGDEASQLRSLLEVSYPMENGVVRNWDDMCHVWDYTFGPKKMDIDPTNTKILLTEPPMNPTKNREKMIEVMFEKYGFDSAYIAIQAVLTLYAQGLISGVVIDSGDGVTHICPVYEEFALPHLTRRLDIAGRDITRYLIKLLLLRGYAFNHSADFETVRIMKEKLCYIGYDIEMEQRLALETTVLVESYTLPDGRVIKVGGERFEAPEALFQPHLINVEGPGIAELAFNTIQAADIDIRPELYKHIVLSGGSTMYPGLPSRLEREIKQLYLERVLKNDTEKLAKFKIRIEDPPRRKDMVFIGGAVLAEVTKDRDGFWMSKQEYQEQGLKVLQKLQKISH

Target ORF information:

RefSeq Version	NM_001103531.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Actin-related protein 2 (Arp2), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103531.2

ORF Insert Sequence:

ATGGACAGCA AGGGTCGAAA TGTTATCGTC TGTGATAACG GCACTGGGTT CGTTAAGTGC 
GGATATGCCG GCAGCAATTT CCCCACCCAC ATTTTTCCCT CGATGGTGGG ACGACCCATG 
ATTCGGGCGG TCAACAAAAT CGGCGATATC GAAGTCAAGG ATCTGATGGT CGGCGATGAG 
GCCTCACAGC TGCGATCCCT GCTGGAGGTC TCCTATCCGA TGGAGAACGG TGTGGTGCGC 
AACTGGGACG ACATGTGCCA CGTGTGGGAC TACACGTTCG GTCCCAAAAA GATGGACATC 
GATCCGACGA ACACAAAAAT CCTGCTAACC GAGCCGCCCA TGAATCCCAC AAAGAATCGC 
GAGAAGATGA TTGAGGTGAT GTTCGAGAAA TATGGCTTCG ATTCGGCGTA CATTGCCATC 
CAGGCCGTGC TGACGCTATA TGCCCAGGGT CTGATCTCTG GCGTGGTGAT CGATTCGGGC 
GATGGTGTAA CGCATATATG TCCCGTCTAC GAGGAGTTTG CCCTGCCCCA TCTGACGCGA 
CGATTGGACA TTGCCGGTCG GGATATCACT CGCTATTTGA TTAAGTTACT GTTACTACGT 
GGCTACGCTT TCAATCATTC CGCCGACTTC GAAACGGTTC GCATAATGAA GGAGAAGCTC 
TGCTACATTG GCTACGACAT CGAGATGGAG CAGCGTTTGG CCCTGGAGAC GACGGTGCTG 
GTGGAATCGT ACACGCTACC CGACGGTCGT GTTATCAAGG TCGGTGGCGA GAGATTTGAG 
GCGCCAGAGG CTCTGTTCCA GCCGCATTTG ATCAACGTCG AGGGACCGGG CATTGCGGAA 
CTGGCCTTCA ACACCATCCA GGCGGCAGAC ATCGACATAC GGCCCGAACT CTACAAGCAC 
ATAGTTCTGT CCGGCGGCTC CACCATGTAT CCGGGTCTTC CCAGTCGGCT GGAGCGTGAG 
ATCAAACAGC TGTACCTGGA GCGGGTACTC AAGAACGATA CGGAAAAGCT GGCGAAATTC 
AAAATACGCA TTGAGGATCC ACCGCGACGC AAGGATATGG TCTTTATCGG CGGAGCTGTT 
CTGGCGGAGG TGACAAAGGA TCGCGACGGC TTCTGGATGT CCAAGCAGGA GTATCAGGAA 
CAGGGTCTCA AAGTATTGCA AAAGCTGCAA AAGATCAGTC ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Arp2 ( NM_078648.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523372.2 Drosophila melanogaster Actin-related protein 2 (Arp2), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa03033
Clone ID Related Accession (Same CDS sequence)	NM_001103531.2 , NM_078648.4
Accession Version	NM_078648.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Actin-related protein 2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_078648.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACAGCA AGGGTCGAAA TGTTATCGTC TGTGATAACG GCACTGGGTT CGTTAAGTGC 
GGATATGCCG GCAGCAATTT CCCCACCCAC ATTTTTCCCT CGATGGTGGG ACGACCCATG 
ATTCGGGCGG TCAACAAAAT CGGCGATATC GAAGTCAAGG ATCTGATGGT CGGCGATGAG 
GCCTCACAGC TGCGATCCCT GCTGGAGGTC TCCTATCCGA TGGAGAACGG TGTGGTGCGC 
AACTGGGACG ACATGTGCCA CGTGTGGGAC TACACGTTCG GTCCCAAAAA GATGGACATC 
GATCCGACGA ACACAAAAAT CCTGCTAACC GAGCCGCCCA TGAATCCCAC AAAGAATCGC 
GAGAAGATGA TTGAGGTGAT GTTCGAGAAA TATGGCTTCG ATTCGGCGTA CATTGCCATC 
CAGGCCGTGC TGACGCTATA TGCCCAGGGT CTGATCTCTG GCGTGGTGAT CGATTCGGGC 
GATGGTGTAA CGCATATATG TCCCGTCTAC GAGGAGTTTG CCCTGCCCCA TCTGACGCGA 
CGATTGGACA TTGCCGGTCG GGATATCACT CGCTATTTGA TTAAGTTACT GTTACTACGT 
GGCTACGCTT TCAATCATTC CGCCGACTTC GAAACGGTTC GCATAATGAA GGAGAAGCTC 
TGCTACATTG GCTACGACAT CGAGATGGAG CAGCGTTTGG CCCTGGAGAC GACGGTGCTG 
GTGGAATCGT ACACGCTACC CGACGGTCGT GTTATCAAGG TCGGTGGCGA GAGATTTGAG 
GCGCCAGAGG CTCTGTTCCA GCCGCATTTG ATCAACGTCG AGGGACCGGG CATTGCGGAA 
CTGGCCTTCA ACACCATCCA GGCGGCAGAC ATCGACATAC GGCCCGAACT CTACAAGCAC 
ATAGTTCTGT CCGGCGGCTC CACCATGTAT CCGGGTCTTC CCAGTCGGCT GGAGCGTGAG 
ATCAAACAGC TGTACCTGGA GCGGGTACTC AAGAACGATA CGGAAAAGCT GGCGAAATTC 
AAAATACGCA TTGAGGATCC ACCGCGACGC AAGGATATGG TCTTTATCGG CGGAGCTGTT 
CTGGCGGAGG TGACAAAGGA TCGCGACGGC TTCTGGATGT CCAAGCAGGA GTATCAGGAA 
CAGGGTCTCA AAGTATTGCA AAAGCTGCAA AAGATCAGTC ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523372.2
CDS	578..1762
Translation	MDSKGRNVIVCDNGTGFVKCGYAGSNFPTHIFPSMVGRPMIRAVNKIGDIEVKDLMVGDEASQLRSLLEVSYPMENGVVRNWDDMCHVWDYTFGPKKMDIDPTNTKILLTEPPMNPTKNREKMIEVMFEKYGFDSAYIAIQAVLTLYAQGLISGVVIDSGDGVTHICPVYEEFALPHLTRRLDIAGRDITRYLIKLLLLRGYAFNHSADFETVRIMKEKLCYIGYDIEMEQRLALETTVLVESYTLPDGRVIKVGGERFEAPEALFQPHLINVEGPGIAELAFNTIQAADIDIRPELYKHIVLSGGSTMYPGLPSRLEREIKQLYLERVLKNDTEKLAKFKIRIEDPPRRKDMVFIGGAVLAEVTKDRDGFWMSKQEYQEQGLKVLQKLQKISH

Target ORF information:

RefSeq Version	NM_078648.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Actin-related protein 2 (Arp2), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078648.4

ORF Insert Sequence:

ATGGACAGCA AGGGTCGAAA TGTTATCGTC TGTGATAACG GCACTGGGTT CGTTAAGTGC 
GGATATGCCG GCAGCAATTT CCCCACCCAC ATTTTTCCCT CGATGGTGGG ACGACCCATG 
ATTCGGGCGG TCAACAAAAT CGGCGATATC GAAGTCAAGG ATCTGATGGT CGGCGATGAG 
GCCTCACAGC TGCGATCCCT GCTGGAGGTC TCCTATCCGA TGGAGAACGG TGTGGTGCGC 
AACTGGGACG ACATGTGCCA CGTGTGGGAC TACACGTTCG GTCCCAAAAA GATGGACATC 
GATCCGACGA ACACAAAAAT CCTGCTAACC GAGCCGCCCA TGAATCCCAC AAAGAATCGC 
GAGAAGATGA TTGAGGTGAT GTTCGAGAAA TATGGCTTCG ATTCGGCGTA CATTGCCATC 
CAGGCCGTGC TGACGCTATA TGCCCAGGGT CTGATCTCTG GCGTGGTGAT CGATTCGGGC 
GATGGTGTAA CGCATATATG TCCCGTCTAC GAGGAGTTTG CCCTGCCCCA TCTGACGCGA 
CGATTGGACA TTGCCGGTCG GGATATCACT CGCTATTTGA TTAAGTTACT GTTACTACGT 
GGCTACGCTT TCAATCATTC CGCCGACTTC GAAACGGTTC GCATAATGAA GGAGAAGCTC 
TGCTACATTG GCTACGACAT CGAGATGGAG CAGCGTTTGG CCCTGGAGAC GACGGTGCTG 
GTGGAATCGT ACACGCTACC CGACGGTCGT GTTATCAAGG TCGGTGGCGA GAGATTTGAG 
GCGCCAGAGG CTCTGTTCCA GCCGCATTTG ATCAACGTCG AGGGACCGGG CATTGCGGAA 
CTGGCCTTCA ACACCATCCA GGCGGCAGAC ATCGACATAC GGCCCGAACT CTACAAGCAC 
ATAGTTCTGT CCGGCGGCTC CACCATGTAT CCGGGTCTTC CCAGTCGGCT GGAGCGTGAG 
ATCAAACAGC TGTACCTGGA GCGGGTACTC AAGAACGATA CGGAAAAGCT GGCGAAATTC 
AAAATACGCA TTGAGGATCC ACCGCGACGC AAGGATATGG TCTTTATCGG CGGAGCTGTT 
CTGGCGGAGG TGACAAAGGA TCGCGACGGC TTCTGGATGT CCAAGCAGGA GTATCAGGAA 
CAGGGTCTCA AAGTATTGCA AAAGCTGCAA AAGATCAGTC ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Arp2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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