CG31445 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG31445
Entrez Gene ID	326140
Full Name	CG31445 gene product from transcript CG31445-RB
Synonyms	CG11955,Dmel\CG31445
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 99A1-99A4

mRNA and Protein(s)

mRNA	Protein	Name
NM_143432.3	NP_651689.1	uncharacterized protein, isoform A
NM_001276135.1	NP_001263064.1	uncharacterized protein, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG31445	NP_001263064.1
Drosophila melanogaster (fruit fly)	SP1029	NP_001263065.1
Drosophila melanogaster (fruit fly)	CG11951	NP_651688.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000885	XP_316862.5
Caenorhabditis elegans (roundworm)	T07F10.1	NP_001041161.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG31445 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG31445 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa07230	NM_143432.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG31445), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa07230	NM_001276135.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG31445), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG31445 ( NM_143432.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651689.1 Drosophila melanogaster uncharacterized protein, transcript variant A (CG31445), mRNA.

Price & Availability↑

CloneID	OFa07230
Clone ID Related Accession (Same CDS sequence)	NM_143432.3 , NM_001276135.1
Accession Version	NM_143432.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 31, 2011 this sequence version replaced NM_143432.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGTGGT TTCTCTTGTT CGTTCTGGCT ATTGGCCTGG ATTTATCTGT GGCCAACTCG 
ATTTCTAGGT ATAACCACTA CCGACTGCCA ACTGCCTTAA GACCCCAAAG GTATTACTTA 
CGTATCCTGA CACTTTTGGA AAGTCCTGAT GATCTCCGTT TCGCTGGAAA TGTGCAAATC 
GTGATTAAAG CACTGCAAAA TACCAGGAAC ATAACGCTGC ACTCGAAGAA CCTGACCATC 
GATGAGTCCC GGATCACGCT GCGCCAAATT AGTGGAGCGG GAAATATGGA TAATTGTGTA 
TCCTCCACAT CCGTTAATCC CACCCACGAC TATTACATCT TTCACACCTG CAAGGAACTT 
TTGGCCGGCA ATGTATACAA ACTGTTCCTT CCATTCTCGG CTGATCTAAC ACCTCAACTT 
TTTGGTTACT ACCGCAGTTC GTACAAGGAT CCTGTGACCA ACAAGACACG CTGGCTGTCT 
GCGACGCAAT TCGAACCATC CTCCGCTCGA AAGGCATTTC CCTGCTTCGA TGAACCCGGT 
TTTAAGGCAT CCTTCGTGGT CACTTTGGGC TATCACAAGC AGTTCAATGC ACTGAGCAAT 
ATGCCCGTAA GGGAAATCAG GCCACACGAG TCCCTTGCAA ACTACATATG GTGCGAGTTC 
CAGGAGTCGG TGCCAATGTC CACCTATCTG GTGGCATATT CGGTTAATGA TTTCTCCTTT 
AAACCCTCCA CTCTGCCAAA TGGAGCTCTA TTCCGGACTT GGGCCAGACC CAATGCCATA 
GATCAGTGCG ATTATGCCGC GGAGTTTGGA CCCAAAGTGC TGCAATACTA TGAGGAGTTC 
TTCGGCATTA GGTATCCGCT TCCCAAGATC GATCAGATGG CTGTTCCGGA TTTCAGCGCT 
GGTGCCATGG AGAACTGGGG TCTGGTCAAA TACAGAGAAT CCACACTTCT CTACTCGCCA 
ACCCACTCAT CACTGGCGGA TAAACAGGAT CTGGCCAATG TAATAGCTCA TGAGTTGGCC 
CACCAATGGT TCGGTAATCT GGTGACGATG AAGTGGTGGA CCGACCTCTG GCTAAACGAG 
GGATTCGCCA CATATGTGGC CGGCTTGGGA GTACAGGAAA TCTATCCGGA GTGGCACTCA 
AGGGATAAGG GCTCGCTGAC AGCTCTGATG ACCGCCTTTC GCCTGGACTC ACTGGTTAGC 
AGCCATCCCA TATCGAGGCC CATTCAAATG GTGACGGAGA TCGAGGAAAG CTTTGATGCC 
ATCTCTTACC AAAAGGGATC CGCAGTGTTA CGAATGATGC ACTTGTTCAT GGGCGAGGAG 
TCCTTCCGTA CGGGGCTAAG GGAGTATCTT AAGCTTTACG CCTACAAGAA TGCCGAACAG 
AATAATCTAT GGGAGTCACT GACCACAGCA GCTCATCAAA ATGGAGCTCT ACCCGGGCAC 
TATGACATAA ATACCATTAT GGACTCGTGG ACTCTGCAAA CCGGATTTCC TGTGCTTAAT 
ATTACCCGTG ACTATTCGAC TGGAACCGCT GAGATTACCC AGGAACGTTA CTTGCGCAAT 
TCCCAGATAC CACAAGCCGA CCGCGTTGGC TGCTGGTGGG TGCCACTGAG CTATACCACC 
CAGGATGAGA ATGACTTCAA TAACACGTCA CCCAAGGCGT GGATGGAGTG CAGCAGTACG 
GACGAAGGTG TGCCTACGAC CATTGATCAT TCAGCTGGAC CCGAAGAATG GCTGATCCTC 
AACATACAGT TGTCGACTCC TTACAAGGCC AACTATGATG CCAGAAACTG GAAGCTGTTG 
ATTGATACTT TGAACAGCAA AGATTTCCAG AGCATTCACG TGATCAACAG AGCCCAGCTT 
ATAGACGATG TTCTGTACTT CGCCTGGACG GGGGAACAGG ACTACGAAAC CGCACTGCAA 
GTGACCAACT ATTTGCGGAG GGAACGAGAC CTTATTCCTT GGAAATCAGC CCTGGACAAT 
TTGAAGTTGT TGAACCGCAT TCTGCGACAA ACTCCCAACT TTGGGTCATT TAAACGCTAC 
ATGCAGAAGC TTCTTACTCC GATCTACGAG CACCTGCACG GCATGAACGA CACCTTTAGT 
TTGATGACGC AGCAGGATGA GGTCCTTTTG AAGACCACGG TGGTCAATGT GGCGTGCCAG 
TACGATGTTT CCGATTGTGT GACCCAGGCG CAGGCCTATT TTCGCCGATG GCGGGCAGAG 
ACCAATCCTG ACGAGAACCA TCCGGTTCCA TTAAACCTTC GCAGCACAGT GTACTGCACC 
GCAATAAGTC AAGGCACAGA GGAGGATTGG AACTTCCTGT GGTCGCGGTA CCGGAAATCA 
AATGTGGCAT CCGAGAGGCA GACCATCTTG AGTACCCTGG GCTGCTCGAA GGAGGTTTGG 
ATACTGCAGC GTTACATGGA AAAGTCCTTC CACCCCAAGA GCGCCATTCG CAAGCAGGAC 
TCATCGCTGT GCTTTCAGGC GGTGGCCTCC AGAGAGGTGG GCTTCCTCCT GGCCAAGCAG 
TACTTCATAG AGAATGTTGA GCTTATCCAC AAATACTATT ATCCGCAGAC GAGAGTCATG 
TCCCGACTGT TGACGCCGTT ATCCGAGCAA GTTAGCACAA TAAATGATCT GAACAAGTTC 
AGGTCCTTTG CCAACGATTC CCGACATTTC CTGAAGGGCA TTCGTCAGGC GATGCAGCAG 
AGCCTGGAGA CGATGCTCAC GAATGTGCAG TGGATGGATT GGAACTATCA TAAATTCTCA 
CGGACTATAC GCCACCATTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651689.1
CDS	59..2842
Translation	MKWFLLFVLAIGLDLSVANSISRYNHYRLPTALRPQRYYLRILTLLESPDDLRFAGNVQIVIKALQNTRNITLHSKNLTIDESRITLRQISGAGNMDNCVSSTSVNPTHDYYIFHTCKELLAGNVYKLFLPFSADLTPQLFGYYRSSYKDPVTNKTRWLSATQFEPSSARKAFPCFDEPGFKASFVVTLGYHKQFNALSNMPVREIRPHESLANYIWCEFQESVPMSTYLVAYSVNDFSFKPSTLPNGALFRTWARPNAIDQCDYAAEFGPKVLQYYEEFFGIRYPLPKIDQMAVPDFSAGAMENWGLVKYRESTLLYSPTHSSLADKQDLANVIAHELAHQWFGNLVTMKWWTDLWLNEGFATYVAGLGVQEIYPEWHSRDKGSLTALMTAFRLDSLVSSHPISRPIQMVTEIEESFDAISYQKGSAVLRMMHLFMGEESFRTGLREYLKLYAYKNAEQNNLWESLTTAAHQNGALPGHYDINTIMDSWTLQTGFPVLNITRDYSTGTAEITQERYLRNSQIPQADRVGCWWVPLSYTTQDENDFNNTSPKAWMECSSTDEGVPTTIDHSAGPEEWLILNIQLSTPYKANYDARNWKLLIDTLNSKDFQSIHVINRAQLIDDVLYFAWTGEQDYETALQVTNYLRRERDLIPWKSALDNLKLLNRILRQTPNFGSFKRYMQKLLTPIYEHLHGMNDTFSLMTQQDEVLLKTTVVNVACQYDVSDCVTQAQAYFRRWRAETNPDENHPVPLNLRSTVYCTAISQGTEEDWNFLWSRYRKSNVASERQTILSTLGCSKEVWILQRYMEKSFHPKSAIRKQDSSLCFQAVASREVGFLLAKQYFIENVELIHKYYYPQTRVMSRLLTPLSEQVSTINDLNKFRSFANDSRHFLKGIRQAMQQSLETMLTNVQWMDWNYHKFSRTIRHHL

Target ORF information:

RefSeq Version	NM_143432.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG31445), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143432.3

ORF Insert Sequence:

ATGAAGTGGT TTCTCTTGTT CGTTCTGGCT ATTGGCCTGG ATTTATCTGT GGCCAACTCG 
ATTTCTAGGT ATAACCACTA CCGACTGCCA ACTGCCTTAA GACCCCAAAG GTATTACTTA 
CGTATCCTGA CACTTTTGGA AAGTCCTGAT GATCTCCGTT TCGCTGGAAA TGTGCAAATC 
GTGATTAAAG CACTGCAAAA TACCAGGAAC ATAACGCTGC ACTCGAAGAA CCTGACCATC 
GATGAGTCCC GGATCACGCT GCGCCAAATT AGTGGAGCGG GAAATATGGA TAATTGTGTA 
TCCTCCACAT CCGTTAATCC CACCCACGAC TATTACATCT TTCACACCTG CAAGGAACTT 
TTGGCCGGCA ATGTATACAA ACTGTTCCTT CCATTCTCGG CTGATCTAAC ACCTCAACTT 
TTTGGTTACT ACCGCAGTTC GTACAAGGAT CCTGTGACCA ACAAGACACG CTGGCTGTCT 
GCGACGCAAT TCGAACCATC CTCCGCTCGA AAGGCATTTC CCTGCTTCGA TGAACCCGGT 
TTTAAGGCAT CCTTCGTGGT CACTTTGGGC TATCACAAGC AGTTCAATGC ACTGAGCAAT 
ATGCCCGTAA GGGAAATCAG GCCACACGAG TCCCTTGCAA ACTACATATG GTGCGAGTTC 
CAGGAGTCGG TGCCAATGTC CACCTATCTG GTGGCATATT CGGTTAATGA TTTCTCCTTT 
AAACCCTCCA CTCTGCCAAA TGGAGCTCTA TTCCGGACTT GGGCCAGACC CAATGCCATA 
GATCAGTGCG ATTATGCCGC GGAGTTTGGA CCCAAAGTGC TGCAATACTA TGAGGAGTTC 
TTCGGCATTA GGTATCCGCT TCCCAAGATC GATCAGATGG CTGTTCCGGA TTTCAGCGCT 
GGTGCCATGG AGAACTGGGG TCTGGTCAAA TACAGAGAAT CCACACTTCT CTACTCGCCA 
ACCCACTCAT CACTGGCGGA TAAACAGGAT CTGGCCAATG TAATAGCTCA TGAGTTGGCC 
CACCAATGGT TCGGTAATCT GGTGACGATG AAGTGGTGGA CCGACCTCTG GCTAAACGAG 
GGATTCGCCA CATATGTGGC CGGCTTGGGA GTACAGGAAA TCTATCCGGA GTGGCACTCA 
AGGGATAAGG GCTCGCTGAC AGCTCTGATG ACCGCCTTTC GCCTGGACTC ACTGGTTAGC 
AGCCATCCCA TATCGAGGCC CATTCAAATG GTGACGGAGA TCGAGGAAAG CTTTGATGCC 
ATCTCTTACC AAAAGGGATC CGCAGTGTTA CGAATGATGC ACTTGTTCAT GGGCGAGGAG 
TCCTTCCGTA CGGGGCTAAG GGAGTATCTT AAGCTTTACG CCTACAAGAA TGCCGAACAG 
AATAATCTAT GGGAGTCACT GACCACAGCA GCTCATCAAA ATGGAGCTCT ACCCGGGCAC 
TATGACATAA ATACCATTAT GGACTCGTGG ACTCTGCAAA CCGGATTTCC TGTGCTTAAT 
ATTACCCGTG ACTATTCGAC TGGAACCGCT GAGATTACCC AGGAACGTTA CTTGCGCAAT 
TCCCAGATAC CACAAGCCGA CCGCGTTGGC TGCTGGTGGG TGCCACTGAG CTATACCACC 
CAGGATGAGA ATGACTTCAA TAACACGTCA CCCAAGGCGT GGATGGAGTG CAGCAGTACG 
GACGAAGGTG TGCCTACGAC CATTGATCAT TCAGCTGGAC CCGAAGAATG GCTGATCCTC 
AACATACAGT TGTCGACTCC TTACAAGGCC AACTATGATG CCAGAAACTG GAAGCTGTTG 
ATTGATACTT TGAACAGCAA AGATTTCCAG AGCATTCACG TGATCAACAG AGCCCAGCTT 
ATAGACGATG TTCTGTACTT CGCCTGGACG GGGGAACAGG ACTACGAAAC CGCACTGCAA 
GTGACCAACT ATTTGCGGAG GGAACGAGAC CTTATTCCTT GGAAATCAGC CCTGGACAAT 
TTGAAGTTGT TGAACCGCAT TCTGCGACAA ACTCCCAACT TTGGGTCATT TAAACGCTAC 
ATGCAGAAGC TTCTTACTCC GATCTACGAG CACCTGCACG GCATGAACGA CACCTTTAGT 
TTGATGACGC AGCAGGATGA GGTCCTTTTG AAGACCACGG TGGTCAATGT GGCGTGCCAG 
TACGATGTTT CCGATTGTGT GACCCAGGCG CAGGCCTATT TTCGCCGATG GCGGGCAGAG 
ACCAATCCTG ACGAGAACCA TCCGGTTCCA TTAAACCTTC GCAGCACAGT GTACTGCACC 
GCAATAAGTC AAGGCACAGA GGAGGATTGG AACTTCCTGT GGTCGCGGTA CCGGAAATCA 
AATGTGGCAT CCGAGAGGCA GACCATCTTG AGTACCCTGG GCTGCTCGAA GGAGGTTTGG 
ATACTGCAGC GTTACATGGA AAAGTCCTTC CACCCCAAGA GCGCCATTCG CAAGCAGGAC 
TCATCGCTGT GCTTTCAGGC GGTGGCCTCC AGAGAGGTGG GCTTCCTCCT GGCCAAGCAG 
TACTTCATAG AGAATGTTGA GCTTATCCAC AAATACTATT ATCCGCAGAC GAGAGTCATG 
TCCCGACTGT TGACGCCGTT ATCCGAGCAA GTTAGCACAA TAAATGATCT GAACAAGTTC 
AGGTCCTTTG CCAACGATTC CCGACATTTC CTGAAGGGCA TTCGTCAGGC GATGCAGCAG 
AGCCTGGAGA CGATGCTCAC GAATGTGCAG TGGATGGATT GGAACTATCA TAAATTCTCA 
CGGACTATAC GCCACCATTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG31445 ( NM_001276135.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001263064.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG31445), mRNA.

Price & Availability↑

CloneID	OFa07230
Clone ID Related Accession (Same CDS sequence)	NM_143432.3 , NM_001276135.1
Accession Version	NM_001276135.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGTGGT TTCTCTTGTT CGTTCTGGCT ATTGGCCTGG ATTTATCTGT GGCCAACTCG 
ATTTCTAGGT ATAACCACTA CCGACTGCCA ACTGCCTTAA GACCCCAAAG GTATTACTTA 
CGTATCCTGA CACTTTTGGA AAGTCCTGAT GATCTCCGTT TCGCTGGAAA TGTGCAAATC 
GTGATTAAAG CACTGCAAAA TACCAGGAAC ATAACGCTGC ACTCGAAGAA CCTGACCATC 
GATGAGTCCC GGATCACGCT GCGCCAAATT AGTGGAGCGG GAAATATGGA TAATTGTGTA 
TCCTCCACAT CCGTTAATCC CACCCACGAC TATTACATCT TTCACACCTG CAAGGAACTT 
TTGGCCGGCA ATGTATACAA ACTGTTCCTT CCATTCTCGG CTGATCTAAC ACCTCAACTT 
TTTGGTTACT ACCGCAGTTC GTACAAGGAT CCTGTGACCA ACAAGACACG CTGGCTGTCT 
GCGACGCAAT TCGAACCATC CTCCGCTCGA AAGGCATTTC CCTGCTTCGA TGAACCCGGT 
TTTAAGGCAT CCTTCGTGGT CACTTTGGGC TATCACAAGC AGTTCAATGC ACTGAGCAAT 
ATGCCCGTAA GGGAAATCAG GCCACACGAG TCCCTTGCAA ACTACATATG GTGCGAGTTC 
CAGGAGTCGG TGCCAATGTC CACCTATCTG GTGGCATATT CGGTTAATGA TTTCTCCTTT 
AAACCCTCCA CTCTGCCAAA TGGAGCTCTA TTCCGGACTT GGGCCAGACC CAATGCCATA 
GATCAGTGCG ATTATGCCGC GGAGTTTGGA CCCAAAGTGC TGCAATACTA TGAGGAGTTC 
TTCGGCATTA GGTATCCGCT TCCCAAGATC GATCAGATGG CTGTTCCGGA TTTCAGCGCT 
GGTGCCATGG AGAACTGGGG TCTGGTCAAA TACAGAGAAT CCACACTTCT CTACTCGCCA 
ACCCACTCAT CACTGGCGGA TAAACAGGAT CTGGCCAATG TAATAGCTCA TGAGTTGGCC 
CACCAATGGT TCGGTAATCT GGTGACGATG AAGTGGTGGA CCGACCTCTG GCTAAACGAG 
GGATTCGCCA CATATGTGGC CGGCTTGGGA GTACAGGAAA TCTATCCGGA GTGGCACTCA 
AGGGATAAGG GCTCGCTGAC AGCTCTGATG ACCGCCTTTC GCCTGGACTC ACTGGTTAGC 
AGCCATCCCA TATCGAGGCC CATTCAAATG GTGACGGAGA TCGAGGAAAG CTTTGATGCC 
ATCTCTTACC AAAAGGGATC CGCAGTGTTA CGAATGATGC ACTTGTTCAT GGGCGAGGAG 
TCCTTCCGTA CGGGGCTAAG GGAGTATCTT AAGCTTTACG CCTACAAGAA TGCCGAACAG 
AATAATCTAT GGGAGTCACT GACCACAGCA GCTCATCAAA ATGGAGCTCT ACCCGGGCAC 
TATGACATAA ATACCATTAT GGACTCGTGG ACTCTGCAAA CCGGATTTCC TGTGCTTAAT 
ATTACCCGTG ACTATTCGAC TGGAACCGCT GAGATTACCC AGGAACGTTA CTTGCGCAAT 
TCCCAGATAC CACAAGCCGA CCGCGTTGGC TGCTGGTGGG TGCCACTGAG CTATACCACC 
CAGGATGAGA ATGACTTCAA TAACACGTCA CCCAAGGCGT GGATGGAGTG CAGCAGTACG 
GACGAAGGTG TGCCTACGAC CATTGATCAT TCAGCTGGAC CCGAAGAATG GCTGATCCTC 
AACATACAGT TGTCGACTCC TTACAAGGCC AACTATGATG CCAGAAACTG GAAGCTGTTG 
ATTGATACTT TGAACAGCAA AGATTTCCAG AGCATTCACG TGATCAACAG AGCCCAGCTT 
ATAGACGATG TTCTGTACTT CGCCTGGACG GGGGAACAGG ACTACGAAAC CGCACTGCAA 
GTGACCAACT ATTTGCGGAG GGAACGAGAC CTTATTCCTT GGAAATCAGC CCTGGACAAT 
TTGAAGTTGT TGAACCGCAT TCTGCGACAA ACTCCCAACT TTGGGTCATT TAAACGCTAC 
ATGCAGAAGC TTCTTACTCC GATCTACGAG CACCTGCACG GCATGAACGA CACCTTTAGT 
TTGATGACGC AGCAGGATGA GGTCCTTTTG AAGACCACGG TGGTCAATGT GGCGTGCCAG 
TACGATGTTT CCGATTGTGT GACCCAGGCG CAGGCCTATT TTCGCCGATG GCGGGCAGAG 
ACCAATCCTG ACGAGAACCA TCCGGTTCCA TTAAACCTTC GCAGCACAGT GTACTGCACC 
GCAATAAGTC AAGGCACAGA GGAGGATTGG AACTTCCTGT GGTCGCGGTA CCGGAAATCA 
AATGTGGCAT CCGAGAGGCA GACCATCTTG AGTACCCTGG GCTGCTCGAA GGAGGTTTGG 
ATACTGCAGC GTTACATGGA AAAGTCCTTC CACCCCAAGA GCGCCATTCG CAAGCAGGAC 
TCATCGCTGT GCTTTCAGGC GGTGGCCTCC AGAGAGGTGG GCTTCCTCCT GGCCAAGCAG 
TACTTCATAG AGAATGTTGA GCTTATCCAC AAATACTATT ATCCGCAGAC GAGAGTCATG 
TCCCGACTGT TGACGCCGTT ATCCGAGCAA GTTAGCACAA TAAATGATCT GAACAAGTTC 
AGGTCCTTTG CCAACGATTC CCGACATTTC CTGAAGGGCA TTCGTCAGGC GATGCAGCAG 
AGCCTGGAGA CGATGCTCAC GAATGTGCAG TGGATGGATT GGAACTATCA TAAATTCTCA 
CGGACTATAC GCCACCATTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263064.1
CDS	197..2980
Translation	MKWFLLFVLAIGLDLSVANSISRYNHYRLPTALRPQRYYLRILTLLESPDDLRFAGNVQIVIKALQNTRNITLHSKNLTIDESRITLRQISGAGNMDNCVSSTSVNPTHDYYIFHTCKELLAGNVYKLFLPFSADLTPQLFGYYRSSYKDPVTNKTRWLSATQFEPSSARKAFPCFDEPGFKASFVVTLGYHKQFNALSNMPVREIRPHESLANYIWCEFQESVPMSTYLVAYSVNDFSFKPSTLPNGALFRTWARPNAIDQCDYAAEFGPKVLQYYEEFFGIRYPLPKIDQMAVPDFSAGAMENWGLVKYRESTLLYSPTHSSLADKQDLANVIAHELAHQWFGNLVTMKWWTDLWLNEGFATYVAGLGVQEIYPEWHSRDKGSLTALMTAFRLDSLVSSHPISRPIQMVTEIEESFDAISYQKGSAVLRMMHLFMGEESFRTGLREYLKLYAYKNAEQNNLWESLTTAAHQNGALPGHYDINTIMDSWTLQTGFPVLNITRDYSTGTAEITQERYLRNSQIPQADRVGCWWVPLSYTTQDENDFNNTSPKAWMECSSTDEGVPTTIDHSAGPEEWLILNIQLSTPYKANYDARNWKLLIDTLNSKDFQSIHVINRAQLIDDVLYFAWTGEQDYETALQVTNYLRRERDLIPWKSALDNLKLLNRILRQTPNFGSFKRYMQKLLTPIYEHLHGMNDTFSLMTQQDEVLLKTTVVNVACQYDVSDCVTQAQAYFRRWRAETNPDENHPVPLNLRSTVYCTAISQGTEEDWNFLWSRYRKSNVASERQTILSTLGCSKEVWILQRYMEKSFHPKSAIRKQDSSLCFQAVASREVGFLLAKQYFIENVELIHKYYYPQTRVMSRLLTPLSEQVSTINDLNKFRSFANDSRHFLKGIRQAMQQSLETMLTNVQWMDWNYHKFSRTIRHHL

Target ORF information:

RefSeq Version	NM_001276135.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG31445), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276135.1

ORF Insert Sequence:

ATGAAGTGGT TTCTCTTGTT CGTTCTGGCT ATTGGCCTGG ATTTATCTGT GGCCAACTCG 
ATTTCTAGGT ATAACCACTA CCGACTGCCA ACTGCCTTAA GACCCCAAAG GTATTACTTA 
CGTATCCTGA CACTTTTGGA AAGTCCTGAT GATCTCCGTT TCGCTGGAAA TGTGCAAATC 
GTGATTAAAG CACTGCAAAA TACCAGGAAC ATAACGCTGC ACTCGAAGAA CCTGACCATC 
GATGAGTCCC GGATCACGCT GCGCCAAATT AGTGGAGCGG GAAATATGGA TAATTGTGTA 
TCCTCCACAT CCGTTAATCC CACCCACGAC TATTACATCT TTCACACCTG CAAGGAACTT 
TTGGCCGGCA ATGTATACAA ACTGTTCCTT CCATTCTCGG CTGATCTAAC ACCTCAACTT 
TTTGGTTACT ACCGCAGTTC GTACAAGGAT CCTGTGACCA ACAAGACACG CTGGCTGTCT 
GCGACGCAAT TCGAACCATC CTCCGCTCGA AAGGCATTTC CCTGCTTCGA TGAACCCGGT 
TTTAAGGCAT CCTTCGTGGT CACTTTGGGC TATCACAAGC AGTTCAATGC ACTGAGCAAT 
ATGCCCGTAA GGGAAATCAG GCCACACGAG TCCCTTGCAA ACTACATATG GTGCGAGTTC 
CAGGAGTCGG TGCCAATGTC CACCTATCTG GTGGCATATT CGGTTAATGA TTTCTCCTTT 
AAACCCTCCA CTCTGCCAAA TGGAGCTCTA TTCCGGACTT GGGCCAGACC CAATGCCATA 
GATCAGTGCG ATTATGCCGC GGAGTTTGGA CCCAAAGTGC TGCAATACTA TGAGGAGTTC 
TTCGGCATTA GGTATCCGCT TCCCAAGATC GATCAGATGG CTGTTCCGGA TTTCAGCGCT 
GGTGCCATGG AGAACTGGGG TCTGGTCAAA TACAGAGAAT CCACACTTCT CTACTCGCCA 
ACCCACTCAT CACTGGCGGA TAAACAGGAT CTGGCCAATG TAATAGCTCA TGAGTTGGCC 
CACCAATGGT TCGGTAATCT GGTGACGATG AAGTGGTGGA CCGACCTCTG GCTAAACGAG 
GGATTCGCCA CATATGTGGC CGGCTTGGGA GTACAGGAAA TCTATCCGGA GTGGCACTCA 
AGGGATAAGG GCTCGCTGAC AGCTCTGATG ACCGCCTTTC GCCTGGACTC ACTGGTTAGC 
AGCCATCCCA TATCGAGGCC CATTCAAATG GTGACGGAGA TCGAGGAAAG CTTTGATGCC 
ATCTCTTACC AAAAGGGATC CGCAGTGTTA CGAATGATGC ACTTGTTCAT GGGCGAGGAG 
TCCTTCCGTA CGGGGCTAAG GGAGTATCTT AAGCTTTACG CCTACAAGAA TGCCGAACAG 
AATAATCTAT GGGAGTCACT GACCACAGCA GCTCATCAAA ATGGAGCTCT ACCCGGGCAC 
TATGACATAA ATACCATTAT GGACTCGTGG ACTCTGCAAA CCGGATTTCC TGTGCTTAAT 
ATTACCCGTG ACTATTCGAC TGGAACCGCT GAGATTACCC AGGAACGTTA CTTGCGCAAT 
TCCCAGATAC CACAAGCCGA CCGCGTTGGC TGCTGGTGGG TGCCACTGAG CTATACCACC 
CAGGATGAGA ATGACTTCAA TAACACGTCA CCCAAGGCGT GGATGGAGTG CAGCAGTACG 
GACGAAGGTG TGCCTACGAC CATTGATCAT TCAGCTGGAC CCGAAGAATG GCTGATCCTC 
AACATACAGT TGTCGACTCC TTACAAGGCC AACTATGATG CCAGAAACTG GAAGCTGTTG 
ATTGATACTT TGAACAGCAA AGATTTCCAG AGCATTCACG TGATCAACAG AGCCCAGCTT 
ATAGACGATG TTCTGTACTT CGCCTGGACG GGGGAACAGG ACTACGAAAC CGCACTGCAA 
GTGACCAACT ATTTGCGGAG GGAACGAGAC CTTATTCCTT GGAAATCAGC CCTGGACAAT 
TTGAAGTTGT TGAACCGCAT TCTGCGACAA ACTCCCAACT TTGGGTCATT TAAACGCTAC 
ATGCAGAAGC TTCTTACTCC GATCTACGAG CACCTGCACG GCATGAACGA CACCTTTAGT 
TTGATGACGC AGCAGGATGA GGTCCTTTTG AAGACCACGG TGGTCAATGT GGCGTGCCAG 
TACGATGTTT CCGATTGTGT GACCCAGGCG CAGGCCTATT TTCGCCGATG GCGGGCAGAG 
ACCAATCCTG ACGAGAACCA TCCGGTTCCA TTAAACCTTC GCAGCACAGT GTACTGCACC 
GCAATAAGTC AAGGCACAGA GGAGGATTGG AACTTCCTGT GGTCGCGGTA CCGGAAATCA 
AATGTGGCAT CCGAGAGGCA GACCATCTTG AGTACCCTGG GCTGCTCGAA GGAGGTTTGG 
ATACTGCAGC GTTACATGGA AAAGTCCTTC CACCCCAAGA GCGCCATTCG CAAGCAGGAC 
TCATCGCTGT GCTTTCAGGC GGTGGCCTCC AGAGAGGTGG GCTTCCTCCT GGCCAAGCAG 
TACTTCATAG AGAATGTTGA GCTTATCCAC AAATACTATT ATCCGCAGAC GAGAGTCATG 
TCCCGACTGT TGACGCCGTT ATCCGAGCAA GTTAGCACAA TAAATGATCT GAACAAGTTC 
AGGTCCTTTG CCAACGATTC CCGACATTTC CTGAAGGGCA TTCGTCAGGC GATGCAGCAG 
AGCCTGGAGA CGATGCTCAC GAATGTGCAG TGGATGGATT GGAACTATCA TAAATTCTCA 
CGGACTATAC GCCACCATTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG31445 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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