SP1029 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	SP1029
Entrez Gene ID	53473
Full Name	CG11956 gene product from transcript CG11956-RA
Synonyms	CG11956,Dmel\CG11956,ptl,ptls
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 99A6-99A6

mRNA and Protein(s)

mRNA	Protein	Name
NM_170405.2	NP_733284.1	SP1029, isoform B
NM_144361.2	NP_652618.1	SP1029, isoform A
NM_001300651.1	NP_001287580.1	SP1029, isoform E
NM_170406.2	NP_733285.1	SP1029, isoform C
NM_001276136.1	NP_001263065.1	SP1029, isoform D

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG31445	NP_001263064.1
Drosophila melanogaster (fruit fly)	SP1029	NP_001263065.1
Drosophila melanogaster (fruit fly)	CG11951	NP_651688.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000885	XP_316862.5
Caenorhabditis elegans (roundworm)	T07F10.1	NP_001041161.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SP1029 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SP1029 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa07015	NM_144361.2 Latest version!	Drosophila melanogaster SP1029, transcript variant A (SP1029), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa07015	NM_170405.2 Latest version!	Drosophila melanogaster SP1029, transcript variant B (SP1029), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa07015	NM_170406.2 Latest version!	Drosophila melanogaster SP1029, transcript variant C (SP1029), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa07015	NM_001276136.1 Latest version!	Drosophila melanogaster SP1029, transcript variant D (SP1029), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa07015	NM_001300651.1 Latest version!	Drosophila melanogaster SP1029, transcript variant E (SP1029), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SP1029 ( NM_170405.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733284.1 Drosophila melanogaster SP1029, transcript variant B (SP1029), mRNA.

Price & Availability↑

CloneID	OFa07015
Clone ID Related Accession (Same CDS sequence)	NM_170405.2 , NM_144361.2 , NM_001300651.1 , NM_170406.2 , NM_001276136.1
Accession Version	NM_170405.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2799bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	SP1029, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170405.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGTGGT TTCTGCTGTT CGTGGCGGCC CTTGGCCTGG GATTAGCCAC CGCCGACTCA 
TCCGCCGACT CCATCGAGAC CACCTACAAC TACTACCGCC TGCCCACATC GCTGCGCCCG 
CAGAAGTACC ATCTCCGCAT TCTGACACTG CTGGAGAATC CGGAGGACCT GCGTTTTTCC 
GGTTCCGTAA AGATCCTGAT CGAAGCCCTG GAAAACACCA AGAACGTAAC GCTGCACTCC 
AAGAATCTGA CCATCGATGA GTCCCAGATC ACCCTGCGCC AGATCGGCGG AGAGGGCAAG 
AAGGAGAACT GCGTGTCCTC CACGGCGGTG AATCCATCCC ATGACTTCTA CATCCTCAAC 
ACCTGCCAGG AACTTTTGGC CGGCAACACC TACGAGTTGT ACATGCCCTT CGCCGCCGAC 
CTGAACCGAC AACTTGAGGG CTATTACCGC AGCTCCTACA AGGATCCCGT GGCCAATCTT 
ACCAAATGGA TCTCGGTTAC CCAGTTTGAA CCGGCATCGG CTCGATTGGC CTTCCCCTGC 
TTTGATGAGC CCGATTTCAA GGCTCCTTTC GTGGTTACCC TTGGATATCA TAAGAAGTAT 
ACGGCTATAA GCAACATGCC CGAGAAGGAA ACCAAGCCAC ATGAAACCCT CGCCGACTAC 
ATATGGTGCG AGTTCCAGGA GTCGGTGCCC ATGTCCACCT ACCTGGTTGC CTACTCCGTG 
AACGATTTCT CGCACAAGCC CTCCACCCTG CCAAATAGCG CTCTATTCCG AACTTGGGCC 
AGACCCAATG CCATCGATCA GTGCGACTAT GCCGCTCAGT TCGGACCCAA AGTGTTGCAA 
TACTATGAGC AGTTCTTCGG CATCAAGTTC CCGCTACCCA AGATCGATCA GATTGCAGTG 
CCCGATTTCA GTGCCGGCGC CATGGAGAAC TGGGGTCTGG TGACCTACAG GGAGATTGCC 
CTGCTCTACT CGGCGGCACA CTCCTCGCTG GCGGATAAGC AACGAGTGGC CAGCGTGGTG 
GCTCATGAGT TGGCGCACCA GTGGTTTGGT AACCTGGTCA CCATGAAGTG GTGGACCGAC 
CTGTGGCTGA ACGAGGGATT CGCCACCTAT GTGGCCAGTT TGGGTGTGGA GAATATCAAT 
CCGGAATGGC GTTCCATGGA GCAGGAGTCG CTGTCCAATC TGCTGACCAT TTTCCGCAGA 
GACGCCCTGG AGAGCAGCCA TCCCATCTCG AGACCCATCC AAATGGTGTC GGAGATCTCG 
GAGAGCTTCG ATCAGATCTC ATACCAGAAG GGCTCCACGG TGCTGCGCAT GATGCACTTG 
TTCCTGGGCG AGGAGTCCTT CCGCTCCGGT CTGCAGGCCT ATCTGCAGAA GTTCTCCTAC 
AAGAATGCTG AGCAGGACAA TCTCTGGGAG TCGCTCACCC AGGCAGCTCA CAAGTATCGA 
TCTCTGCCCA AGAGCTATGA CATCAAGAGC ATCATGGACT CATGGACCCT GCAAACTGGC 
TACCCTGTGA TCAACGTGAC GCGAGACTAC GCTGCCAGGA CGGCCAAGCT CAACCAGGAG 
CGCTATCTCC TGAACACCCA GGTAGCCCGA GCTTATCGCG GCGGTTGCTG GTGGGTGCCA 
CTGAGTTACA CCACACAGGC GGTGCAGGAC TTCAACAACA CGGCACCCAA GGCCTGGATG 
GAGTGCGGCA AGAATGGCGA GAGCCTGCCC AAGACCATTC AGGACCTGCC AGGACCCGAT 
CAGTGGGTGA TCTTTAACAC GCAGCTCTCG ACGCTGTACA AGGTCAACTA CGATGCCCAG 
AACTGGAAGC TTCTTATTGA GACCTTGACC AATGGAGATT TCGAACGCAT TCACGTGATC 
AACCGAGCTC AGCTGATCGA CGATGCCCTA TATCTGGCCT GGACCGGCGA ACAGGACTAC 
GAGATCGCCA TGAGACTGAT CGAGTATCTG CAGCGGGAGC GGGAGTATTT GCCCTGGAAG 
TCGGCCTTCG AGAATCTGAA GCGTGTGGGC CGCATTGTCC GCCAGACACC CGATTTCGAG 
TTCTTCAAGC GCTACATGAA GAAGCTGATT CTGCCGATCT ACGAGCACCT GAATGGCATC 
AACGACACAT TCAGCGCGAT TCCGCAGCAG GATCAGGTGC TGCTGAAGAC AATGGTGGTC 
AACTGGGCGT GTCAGTACCA GGTGGGCGAT TGTGTGCCCC AGGCCCTGGC CTACTACCGC 
AACTGGCGAG CGGAGGCTAA TCCCGACGAG AAGAATCCGG TTCCGATCAA CGTTCGTAGC 
ACTGTGTACT GCACCTCCAT AAAGCACGGA TCCGATTCGG ATTGGGAATT CCTGTGGACG 
CGTTACAAGA AGTCGAATGT GGCGGCTGAG AAGCGCACCA TTCTGACTGC TTTGGGTTGT 
TCGAGGGAAG TGTGGCTGCT GCAGCGTTAT CTGGAGCTGA CCTTTGACCC CAAGGAGGCC 
ATTCGCAAGC AGGACTCGAT GTGGGCCTTC CAAGCGGTGG CCTTCAATGA GGTGGGCTTC 
CTGCTGGCCA AAAAGTATTT CATGGACAAC GTCGACTTCA TCTACAAGTT CTACCATCCG 
CTCACCAAGG ACATGTCCCG CCTACTCTCA CCGCTTTCCG AGCAGGTCAT CACGCTGAGC 
GACTTCAACG AGTTCAAGGA CTTCGTGAAC AACTCCCGTC AGTCGCTGAA GGGATTGGAA 
CAGGCCATCC AACAGACACT GGAGATCATG CTCACCAATG TTCAGTGGAA GGAGCGAAAC 
TACCATCAGA TGTCCCGCTC CATCCAGCAA CTGCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733284.1
CDS	394..3192
Translation	MKWFLLFVAALGLGLATADSSADSIETTYNYYRLPTSLRPQKYHLRILTLLENPEDLRFSGSVKILIEALENTKNVTLHSKNLTIDESQITLRQIGGEGKKENCVSSTAVNPSHDFYILNTCQELLAGNTYELYMPFAADLNRQLEGYYRSSYKDPVANLTKWISVTQFEPASARLAFPCFDEPDFKAPFVVTLGYHKKYTAISNMPEKETKPHETLADYIWCEFQESVPMSTYLVAYSVNDFSHKPSTLPNSALFRTWARPNAIDQCDYAAQFGPKVLQYYEQFFGIKFPLPKIDQIAVPDFSAGAMENWGLVTYREIALLYSAAHSSLADKQRVASVVAHELAHQWFGNLVTMKWWTDLWLNEGFATYVASLGVENINPEWRSMEQESLSNLLTIFRRDALESSHPISRPIQMVSEISESFDQISYQKGSTVLRMMHLFLGEESFRSGLQAYLQKFSYKNAEQDNLWESLTQAAHKYRSLPKSYDIKSIMDSWTLQTGYPVINVTRDYAARTAKLNQERYLLNTQVARAYRGGCWWVPLSYTTQAVQDFNNTAPKAWMECGKNGESLPKTIQDLPGPDQWVIFNTQLSTLYKVNYDAQNWKLLIETLTNGDFERIHVINRAQLIDDALYLAWTGEQDYEIAMRLIEYLQREREYLPWKSAFENLKRVGRIVRQTPDFEFFKRYMKKLILPIYEHLNGINDTFSAIPQQDQVLLKTMVVNWACQYQVGDCVPQALAYYRNWRAEANPDEKNPVPINVRSTVYCTSIKHGSDSDWEFLWTRYKKSNVAAEKRTILTALGCSREVWLLQRYLELTFDPKEAIRKQDSMWAFQAVAFNEVGFLLAKKYFMDNVDFIYKFYHPLTKDMSRLLSPLSEQVITLSDFNEFKDFVNNSRQSLKGLEQAIQQTLEIMLTNVQWKERNYHQMSRSIQQLL

Target ORF information:

RefSeq Version	NM_170405.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster SP1029, transcript variant B (SP1029), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170405.2

ORF Insert Sequence:

ATGAAGTGGT TTCTGCTGTT CGTGGCGGCC CTTGGCCTGG GATTAGCCAC CGCCGACTCA 
TCCGCCGACT CCATCGAGAC CACCTACAAC TACTACCGCC TGCCCACATC GCTGCGCCCG 
CAGAAGTACC ATCTCCGCAT TCTGACACTG CTGGAGAATC CGGAGGACCT GCGTTTTTCC 
GGTTCCGTAA AGATCCTGAT CGAAGCCCTG GAAAACACCA AGAACGTAAC GCTGCACTCC 
AAGAATCTGA CCATCGATGA GTCCCAGATC ACCCTGCGCC AGATCGGCGG AGAGGGCAAG 
AAGGAGAACT GCGTGTCCTC CACGGCGGTG AATCCATCCC ATGACTTCTA CATCCTCAAC 
ACCTGCCAGG AACTTTTGGC CGGCAACACC TACGAGTTGT ACATGCCCTT CGCCGCCGAC 
CTGAACCGAC AACTTGAGGG CTATTACCGC AGCTCCTACA AGGATCCCGT GGCCAATCTT 
ACCAAATGGA TCTCGGTTAC CCAGTTTGAA CCGGCATCGG CTCGATTGGC CTTCCCCTGC 
TTTGATGAGC CCGATTTCAA GGCTCCTTTC GTGGTTACCC TTGGATATCA TAAGAAGTAT 
ACGGCTATAA GCAACATGCC CGAGAAGGAA ACCAAGCCAC ATGAAACCCT CGCCGACTAC 
ATATGGTGCG AGTTCCAGGA GTCGGTGCCC ATGTCCACCT ACCTGGTTGC CTACTCCGTG 
AACGATTTCT CGCACAAGCC CTCCACCCTG CCAAATAGCG CTCTATTCCG AACTTGGGCC 
AGACCCAATG CCATCGATCA GTGCGACTAT GCCGCTCAGT TCGGACCCAA AGTGTTGCAA 
TACTATGAGC AGTTCTTCGG CATCAAGTTC CCGCTACCCA AGATCGATCA GATTGCAGTG 
CCCGATTTCA GTGCCGGCGC CATGGAGAAC TGGGGTCTGG TGACCTACAG GGAGATTGCC 
CTGCTCTACT CGGCGGCACA CTCCTCGCTG GCGGATAAGC AACGAGTGGC CAGCGTGGTG 
GCTCATGAGT TGGCGCACCA GTGGTTTGGT AACCTGGTCA CCATGAAGTG GTGGACCGAC 
CTGTGGCTGA ACGAGGGATT CGCCACCTAT GTGGCCAGTT TGGGTGTGGA GAATATCAAT 
CCGGAATGGC GTTCCATGGA GCAGGAGTCG CTGTCCAATC TGCTGACCAT TTTCCGCAGA 
GACGCCCTGG AGAGCAGCCA TCCCATCTCG AGACCCATCC AAATGGTGTC GGAGATCTCG 
GAGAGCTTCG ATCAGATCTC ATACCAGAAG GGCTCCACGG TGCTGCGCAT GATGCACTTG 
TTCCTGGGCG AGGAGTCCTT CCGCTCCGGT CTGCAGGCCT ATCTGCAGAA GTTCTCCTAC 
AAGAATGCTG AGCAGGACAA TCTCTGGGAG TCGCTCACCC AGGCAGCTCA CAAGTATCGA 
TCTCTGCCCA AGAGCTATGA CATCAAGAGC ATCATGGACT CATGGACCCT GCAAACTGGC 
TACCCTGTGA TCAACGTGAC GCGAGACTAC GCTGCCAGGA CGGCCAAGCT CAACCAGGAG 
CGCTATCTCC TGAACACCCA GGTAGCCCGA GCTTATCGCG GCGGTTGCTG GTGGGTGCCA 
CTGAGTTACA CCACACAGGC GGTGCAGGAC TTCAACAACA CGGCACCCAA GGCCTGGATG 
GAGTGCGGCA AGAATGGCGA GAGCCTGCCC AAGACCATTC AGGACCTGCC AGGACCCGAT 
CAGTGGGTGA TCTTTAACAC GCAGCTCTCG ACGCTGTACA AGGTCAACTA CGATGCCCAG 
AACTGGAAGC TTCTTATTGA GACCTTGACC AATGGAGATT TCGAACGCAT TCACGTGATC 
AACCGAGCTC AGCTGATCGA CGATGCCCTA TATCTGGCCT GGACCGGCGA ACAGGACTAC 
GAGATCGCCA TGAGACTGAT CGAGTATCTG CAGCGGGAGC GGGAGTATTT GCCCTGGAAG 
TCGGCCTTCG AGAATCTGAA GCGTGTGGGC CGCATTGTCC GCCAGACACC CGATTTCGAG 
TTCTTCAAGC GCTACATGAA GAAGCTGATT CTGCCGATCT ACGAGCACCT GAATGGCATC 
AACGACACAT TCAGCGCGAT TCCGCAGCAG GATCAGGTGC TGCTGAAGAC AATGGTGGTC 
AACTGGGCGT GTCAGTACCA GGTGGGCGAT TGTGTGCCCC AGGCCCTGGC CTACTACCGC 
AACTGGCGAG CGGAGGCTAA TCCCGACGAG AAGAATCCGG TTCCGATCAA CGTTCGTAGC 
ACTGTGTACT GCACCTCCAT AAAGCACGGA TCCGATTCGG ATTGGGAATT CCTGTGGACG 
CGTTACAAGA AGTCGAATGT GGCGGCTGAG AAGCGCACCA TTCTGACTGC TTTGGGTTGT 
TCGAGGGAAG TGTGGCTGCT GCAGCGTTAT CTGGAGCTGA CCTTTGACCC CAAGGAGGCC 
ATTCGCAAGC AGGACTCGAT GTGGGCCTTC CAAGCGGTGG CCTTCAATGA GGTGGGCTTC 
CTGCTGGCCA AAAAGTATTT CATGGACAAC GTCGACTTCA TCTACAAGTT CTACCATCCG 
CTCACCAAGG ACATGTCCCG CCTACTCTCA CCGCTTTCCG AGCAGGTCAT CACGCTGAGC 
GACTTCAACG AGTTCAAGGA CTTCGTGAAC AACTCCCGTC AGTCGCTGAA GGGATTGGAA 
CAGGCCATCC AACAGACACT GGAGATCATG CTCACCAATG TTCAGTGGAA GGAGCGAAAC 
TACCATCAGA TGTCCCGCTC CATCCAGCAA CTGCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SP1029 ( NM_144361.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_652618.1 Drosophila melanogaster SP1029, transcript variant A (SP1029), mRNA.

Price & Availability↑

CloneID	OFa07015
Clone ID Related Accession (Same CDS sequence)	NM_170405.2 , NM_144361.2 , NM_001300651.1 , NM_170406.2 , NM_001276136.1
Accession Version	NM_144361.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2799bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	SP1029, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_144361.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGTGGT TTCTGCTGTT CGTGGCGGCC CTTGGCCTGG GATTAGCCAC CGCCGACTCA 
TCCGCCGACT CCATCGAGAC CACCTACAAC TACTACCGCC TGCCCACATC GCTGCGCCCG 
CAGAAGTACC ATCTCCGCAT TCTGACACTG CTGGAGAATC CGGAGGACCT GCGTTTTTCC 
GGTTCCGTAA AGATCCTGAT CGAAGCCCTG GAAAACACCA AGAACGTAAC GCTGCACTCC 
AAGAATCTGA CCATCGATGA GTCCCAGATC ACCCTGCGCC AGATCGGCGG AGAGGGCAAG 
AAGGAGAACT GCGTGTCCTC CACGGCGGTG AATCCATCCC ATGACTTCTA CATCCTCAAC 
ACCTGCCAGG AACTTTTGGC CGGCAACACC TACGAGTTGT ACATGCCCTT CGCCGCCGAC 
CTGAACCGAC AACTTGAGGG CTATTACCGC AGCTCCTACA AGGATCCCGT GGCCAATCTT 
ACCAAATGGA TCTCGGTTAC CCAGTTTGAA CCGGCATCGG CTCGATTGGC CTTCCCCTGC 
TTTGATGAGC CCGATTTCAA GGCTCCTTTC GTGGTTACCC TTGGATATCA TAAGAAGTAT 
ACGGCTATAA GCAACATGCC CGAGAAGGAA ACCAAGCCAC ATGAAACCCT CGCCGACTAC 
ATATGGTGCG AGTTCCAGGA GTCGGTGCCC ATGTCCACCT ACCTGGTTGC CTACTCCGTG 
AACGATTTCT CGCACAAGCC CTCCACCCTG CCAAATAGCG CTCTATTCCG AACTTGGGCC 
AGACCCAATG CCATCGATCA GTGCGACTAT GCCGCTCAGT TCGGACCCAA AGTGTTGCAA 
TACTATGAGC AGTTCTTCGG CATCAAGTTC CCGCTACCCA AGATCGATCA GATTGCAGTG 
CCCGATTTCA GTGCCGGCGC CATGGAGAAC TGGGGTCTGG TGACCTACAG GGAGATTGCC 
CTGCTCTACT CGGCGGCACA CTCCTCGCTG GCGGATAAGC AACGAGTGGC CAGCGTGGTG 
GCTCATGAGT TGGCGCACCA GTGGTTTGGT AACCTGGTCA CCATGAAGTG GTGGACCGAC 
CTGTGGCTGA ACGAGGGATT CGCCACCTAT GTGGCCAGTT TGGGTGTGGA GAATATCAAT 
CCGGAATGGC GTTCCATGGA GCAGGAGTCG CTGTCCAATC TGCTGACCAT TTTCCGCAGA 
GACGCCCTGG AGAGCAGCCA TCCCATCTCG AGACCCATCC AAATGGTGTC GGAGATCTCG 
GAGAGCTTCG ATCAGATCTC ATACCAGAAG GGCTCCACGG TGCTGCGCAT GATGCACTTG 
TTCCTGGGCG AGGAGTCCTT CCGCTCCGGT CTGCAGGCCT ATCTGCAGAA GTTCTCCTAC 
AAGAATGCTG AGCAGGACAA TCTCTGGGAG TCGCTCACCC AGGCAGCTCA CAAGTATCGA 
TCTCTGCCCA AGAGCTATGA CATCAAGAGC ATCATGGACT CATGGACCCT GCAAACTGGC 
TACCCTGTGA TCAACGTGAC GCGAGACTAC GCTGCCAGGA CGGCCAAGCT CAACCAGGAG 
CGCTATCTCC TGAACACCCA GGTAGCCCGA GCTTATCGCG GCGGTTGCTG GTGGGTGCCA 
CTGAGTTACA CCACACAGGC GGTGCAGGAC TTCAACAACA CGGCACCCAA GGCCTGGATG 
GAGTGCGGCA AGAATGGCGA GAGCCTGCCC AAGACCATTC AGGACCTGCC AGGACCCGAT 
CAGTGGGTGA TCTTTAACAC GCAGCTCTCG ACGCTGTACA AGGTCAACTA CGATGCCCAG 
AACTGGAAGC TTCTTATTGA GACCTTGACC AATGGAGATT TCGAACGCAT TCACGTGATC 
AACCGAGCTC AGCTGATCGA CGATGCCCTA TATCTGGCCT GGACCGGCGA ACAGGACTAC 
GAGATCGCCA TGAGACTGAT CGAGTATCTG CAGCGGGAGC GGGAGTATTT GCCCTGGAAG 
TCGGCCTTCG AGAATCTGAA GCGTGTGGGC CGCATTGTCC GCCAGACACC CGATTTCGAG 
TTCTTCAAGC GCTACATGAA GAAGCTGATT CTGCCGATCT ACGAGCACCT GAATGGCATC 
AACGACACAT TCAGCGCGAT TCCGCAGCAG GATCAGGTGC TGCTGAAGAC AATGGTGGTC 
AACTGGGCGT GTCAGTACCA GGTGGGCGAT TGTGTGCCCC AGGCCCTGGC CTACTACCGC 
AACTGGCGAG CGGAGGCTAA TCCCGACGAG AAGAATCCGG TTCCGATCAA CGTTCGTAGC 
ACTGTGTACT GCACCTCCAT AAAGCACGGA TCCGATTCGG ATTGGGAATT CCTGTGGACG 
CGTTACAAGA AGTCGAATGT GGCGGCTGAG AAGCGCACCA TTCTGACTGC TTTGGGTTGT 
TCGAGGGAAG TGTGGCTGCT GCAGCGTTAT CTGGAGCTGA CCTTTGACCC CAAGGAGGCC 
ATTCGCAAGC AGGACTCGAT GTGGGCCTTC CAAGCGGTGG CCTTCAATGA GGTGGGCTTC 
CTGCTGGCCA AAAAGTATTT CATGGACAAC GTCGACTTCA TCTACAAGTT CTACCATCCG 
CTCACCAAGG ACATGTCCCG CCTACTCTCA CCGCTTTCCG AGCAGGTCAT CACGCTGAGC 
GACTTCAACG AGTTCAAGGA CTTCGTGAAC AACTCCCGTC AGTCGCTGAA GGGATTGGAA 
CAGGCCATCC AACAGACACT GGAGATCATG CTCACCAATG TTCAGTGGAA GGAGCGAAAC 
TACCATCAGA TGTCCCGCTC CATCCAGCAA CTGCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_652618.1
CDS	99..2897
Translation	MKWFLLFVAALGLGLATADSSADSIETTYNYYRLPTSLRPQKYHLRILTLLENPEDLRFSGSVKILIEALENTKNVTLHSKNLTIDESQITLRQIGGEGKKENCVSSTAVNPSHDFYILNTCQELLAGNTYELYMPFAADLNRQLEGYYRSSYKDPVANLTKWISVTQFEPASARLAFPCFDEPDFKAPFVVTLGYHKKYTAISNMPEKETKPHETLADYIWCEFQESVPMSTYLVAYSVNDFSHKPSTLPNSALFRTWARPNAIDQCDYAAQFGPKVLQYYEQFFGIKFPLPKIDQIAVPDFSAGAMENWGLVTYREIALLYSAAHSSLADKQRVASVVAHELAHQWFGNLVTMKWWTDLWLNEGFATYVASLGVENINPEWRSMEQESLSNLLTIFRRDALESSHPISRPIQMVSEISESFDQISYQKGSTVLRMMHLFLGEESFRSGLQAYLQKFSYKNAEQDNLWESLTQAAHKYRSLPKSYDIKSIMDSWTLQTGYPVINVTRDYAARTAKLNQERYLLNTQVARAYRGGCWWVPLSYTTQAVQDFNNTAPKAWMECGKNGESLPKTIQDLPGPDQWVIFNTQLSTLYKVNYDAQNWKLLIETLTNGDFERIHVINRAQLIDDALYLAWTGEQDYEIAMRLIEYLQREREYLPWKSAFENLKRVGRIVRQTPDFEFFKRYMKKLILPIYEHLNGINDTFSAIPQQDQVLLKTMVVNWACQYQVGDCVPQALAYYRNWRAEANPDEKNPVPINVRSTVYCTSIKHGSDSDWEFLWTRYKKSNVAAEKRTILTALGCSREVWLLQRYLELTFDPKEAIRKQDSMWAFQAVAFNEVGFLLAKKYFMDNVDFIYKFYHPLTKDMSRLLSPLSEQVITLSDFNEFKDFVNNSRQSLKGLEQAIQQTLEIMLTNVQWKERNYHQMSRSIQQLL

Target ORF information:

RefSeq Version	NM_144361.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster SP1029, transcript variant A (SP1029), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_144361.2

ORF Insert Sequence:

ATGAAGTGGT TTCTGCTGTT CGTGGCGGCC CTTGGCCTGG GATTAGCCAC CGCCGACTCA 
TCCGCCGACT CCATCGAGAC CACCTACAAC TACTACCGCC TGCCCACATC GCTGCGCCCG 
CAGAAGTACC ATCTCCGCAT TCTGACACTG CTGGAGAATC CGGAGGACCT GCGTTTTTCC 
GGTTCCGTAA AGATCCTGAT CGAAGCCCTG GAAAACACCA AGAACGTAAC GCTGCACTCC 
AAGAATCTGA CCATCGATGA GTCCCAGATC ACCCTGCGCC AGATCGGCGG AGAGGGCAAG 
AAGGAGAACT GCGTGTCCTC CACGGCGGTG AATCCATCCC ATGACTTCTA CATCCTCAAC 
ACCTGCCAGG AACTTTTGGC CGGCAACACC TACGAGTTGT ACATGCCCTT CGCCGCCGAC 
CTGAACCGAC AACTTGAGGG CTATTACCGC AGCTCCTACA AGGATCCCGT GGCCAATCTT 
ACCAAATGGA TCTCGGTTAC CCAGTTTGAA CCGGCATCGG CTCGATTGGC CTTCCCCTGC 
TTTGATGAGC CCGATTTCAA GGCTCCTTTC GTGGTTACCC TTGGATATCA TAAGAAGTAT 
ACGGCTATAA GCAACATGCC CGAGAAGGAA ACCAAGCCAC ATGAAACCCT CGCCGACTAC 
ATATGGTGCG AGTTCCAGGA GTCGGTGCCC ATGTCCACCT ACCTGGTTGC CTACTCCGTG 
AACGATTTCT CGCACAAGCC CTCCACCCTG CCAAATAGCG CTCTATTCCG AACTTGGGCC 
AGACCCAATG CCATCGATCA GTGCGACTAT GCCGCTCAGT TCGGACCCAA AGTGTTGCAA 
TACTATGAGC AGTTCTTCGG CATCAAGTTC CCGCTACCCA AGATCGATCA GATTGCAGTG 
CCCGATTTCA GTGCCGGCGC CATGGAGAAC TGGGGTCTGG TGACCTACAG GGAGATTGCC 
CTGCTCTACT CGGCGGCACA CTCCTCGCTG GCGGATAAGC AACGAGTGGC CAGCGTGGTG 
GCTCATGAGT TGGCGCACCA GTGGTTTGGT AACCTGGTCA CCATGAAGTG GTGGACCGAC 
CTGTGGCTGA ACGAGGGATT CGCCACCTAT GTGGCCAGTT TGGGTGTGGA GAATATCAAT 
CCGGAATGGC GTTCCATGGA GCAGGAGTCG CTGTCCAATC TGCTGACCAT TTTCCGCAGA 
GACGCCCTGG AGAGCAGCCA TCCCATCTCG AGACCCATCC AAATGGTGTC GGAGATCTCG 
GAGAGCTTCG ATCAGATCTC ATACCAGAAG GGCTCCACGG TGCTGCGCAT GATGCACTTG 
TTCCTGGGCG AGGAGTCCTT CCGCTCCGGT CTGCAGGCCT ATCTGCAGAA GTTCTCCTAC 
AAGAATGCTG AGCAGGACAA TCTCTGGGAG TCGCTCACCC AGGCAGCTCA CAAGTATCGA 
TCTCTGCCCA AGAGCTATGA CATCAAGAGC ATCATGGACT CATGGACCCT GCAAACTGGC 
TACCCTGTGA TCAACGTGAC GCGAGACTAC GCTGCCAGGA CGGCCAAGCT CAACCAGGAG 
CGCTATCTCC TGAACACCCA GGTAGCCCGA GCTTATCGCG GCGGTTGCTG GTGGGTGCCA 
CTGAGTTACA CCACACAGGC GGTGCAGGAC TTCAACAACA CGGCACCCAA GGCCTGGATG 
GAGTGCGGCA AGAATGGCGA GAGCCTGCCC AAGACCATTC AGGACCTGCC AGGACCCGAT 
CAGTGGGTGA TCTTTAACAC GCAGCTCTCG ACGCTGTACA AGGTCAACTA CGATGCCCAG 
AACTGGAAGC TTCTTATTGA GACCTTGACC AATGGAGATT TCGAACGCAT TCACGTGATC 
AACCGAGCTC AGCTGATCGA CGATGCCCTA TATCTGGCCT GGACCGGCGA ACAGGACTAC 
GAGATCGCCA TGAGACTGAT CGAGTATCTG CAGCGGGAGC GGGAGTATTT GCCCTGGAAG 
TCGGCCTTCG AGAATCTGAA GCGTGTGGGC CGCATTGTCC GCCAGACACC CGATTTCGAG 
TTCTTCAAGC GCTACATGAA GAAGCTGATT CTGCCGATCT ACGAGCACCT GAATGGCATC 
AACGACACAT TCAGCGCGAT TCCGCAGCAG GATCAGGTGC TGCTGAAGAC AATGGTGGTC 
AACTGGGCGT GTCAGTACCA GGTGGGCGAT TGTGTGCCCC AGGCCCTGGC CTACTACCGC 
AACTGGCGAG CGGAGGCTAA TCCCGACGAG AAGAATCCGG TTCCGATCAA CGTTCGTAGC 
ACTGTGTACT GCACCTCCAT AAAGCACGGA TCCGATTCGG ATTGGGAATT CCTGTGGACG 
CGTTACAAGA AGTCGAATGT GGCGGCTGAG AAGCGCACCA TTCTGACTGC TTTGGGTTGT 
TCGAGGGAAG TGTGGCTGCT GCAGCGTTAT CTGGAGCTGA CCTTTGACCC CAAGGAGGCC 
ATTCGCAAGC AGGACTCGAT GTGGGCCTTC CAAGCGGTGG CCTTCAATGA GGTGGGCTTC 
CTGCTGGCCA AAAAGTATTT CATGGACAAC GTCGACTTCA TCTACAAGTT CTACCATCCG 
CTCACCAAGG ACATGTCCCG CCTACTCTCA CCGCTTTCCG AGCAGGTCAT CACGCTGAGC 
GACTTCAACG AGTTCAAGGA CTTCGTGAAC AACTCCCGTC AGTCGCTGAA GGGATTGGAA 
CAGGCCATCC AACAGACACT GGAGATCATG CTCACCAATG TTCAGTGGAA GGAGCGAAAC 
TACCATCAGA TGTCCCGCTC CATCCAGCAA CTGCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SP1029 ( NM_001300651.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287580.1 Drosophila melanogaster SP1029, transcript variant E (SP1029), mRNA.

Price & Availability↑

CloneID	OFa07015
Clone ID Related Accession (Same CDS sequence)	NM_170405.2 , NM_144361.2 , NM_001300651.1 , NM_170406.2 , NM_001276136.1
Accession Version	NM_001300651.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2799bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	SP1029, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGTGGT TTCTGCTGTT CGTGGCGGCC CTTGGCCTGG GATTAGCCAC CGCCGACTCA 
TCCGCCGACT CCATCGAGAC CACCTACAAC TACTACCGCC TGCCCACATC GCTGCGCCCG 
CAGAAGTACC ATCTCCGCAT TCTGACACTG CTGGAGAATC CGGAGGACCT GCGTTTTTCC 
GGTTCCGTAA AGATCCTGAT CGAAGCCCTG GAAAACACCA AGAACGTAAC GCTGCACTCC 
AAGAATCTGA CCATCGATGA GTCCCAGATC ACCCTGCGCC AGATCGGCGG AGAGGGCAAG 
AAGGAGAACT GCGTGTCCTC CACGGCGGTG AATCCATCCC ATGACTTCTA CATCCTCAAC 
ACCTGCCAGG AACTTTTGGC CGGCAACACC TACGAGTTGT ACATGCCCTT CGCCGCCGAC 
CTGAACCGAC AACTTGAGGG CTATTACCGC AGCTCCTACA AGGATCCCGT GGCCAATCTT 
ACCAAATGGA TCTCGGTTAC CCAGTTTGAA CCGGCATCGG CTCGATTGGC CTTCCCCTGC 
TTTGATGAGC CCGATTTCAA GGCTCCTTTC GTGGTTACCC TTGGATATCA TAAGAAGTAT 
ACGGCTATAA GCAACATGCC CGAGAAGGAA ACCAAGCCAC ATGAAACCCT CGCCGACTAC 
ATATGGTGCG AGTTCCAGGA GTCGGTGCCC ATGTCCACCT ACCTGGTTGC CTACTCCGTG 
AACGATTTCT CGCACAAGCC CTCCACCCTG CCAAATAGCG CTCTATTCCG AACTTGGGCC 
AGACCCAATG CCATCGATCA GTGCGACTAT GCCGCTCAGT TCGGACCCAA AGTGTTGCAA 
TACTATGAGC AGTTCTTCGG CATCAAGTTC CCGCTACCCA AGATCGATCA GATTGCAGTG 
CCCGATTTCA GTGCCGGCGC CATGGAGAAC TGGGGTCTGG TGACCTACAG GGAGATTGCC 
CTGCTCTACT CGGCGGCACA CTCCTCGCTG GCGGATAAGC AACGAGTGGC CAGCGTGGTG 
GCTCATGAGT TGGCGCACCA GTGGTTTGGT AACCTGGTCA CCATGAAGTG GTGGACCGAC 
CTGTGGCTGA ACGAGGGATT CGCCACCTAT GTGGCCAGTT TGGGTGTGGA GAATATCAAT 
CCGGAATGGC GTTCCATGGA GCAGGAGTCG CTGTCCAATC TGCTGACCAT TTTCCGCAGA 
GACGCCCTGG AGAGCAGCCA TCCCATCTCG AGACCCATCC AAATGGTGTC GGAGATCTCG 
GAGAGCTTCG ATCAGATCTC ATACCAGAAG GGCTCCACGG TGCTGCGCAT GATGCACTTG 
TTCCTGGGCG AGGAGTCCTT CCGCTCCGGT CTGCAGGCCT ATCTGCAGAA GTTCTCCTAC 
AAGAATGCTG AGCAGGACAA TCTCTGGGAG TCGCTCACCC AGGCAGCTCA CAAGTATCGA 
TCTCTGCCCA AGAGCTATGA CATCAAGAGC ATCATGGACT CATGGACCCT GCAAACTGGC 
TACCCTGTGA TCAACGTGAC GCGAGACTAC GCTGCCAGGA CGGCCAAGCT CAACCAGGAG 
CGCTATCTCC TGAACACCCA GGTAGCCCGA GCTTATCGCG GCGGTTGCTG GTGGGTGCCA 
CTGAGTTACA CCACACAGGC GGTGCAGGAC TTCAACAACA CGGCACCCAA GGCCTGGATG 
GAGTGCGGCA AGAATGGCGA GAGCCTGCCC AAGACCATTC AGGACCTGCC AGGACCCGAT 
CAGTGGGTGA TCTTTAACAC GCAGCTCTCG ACGCTGTACA AGGTCAACTA CGATGCCCAG 
AACTGGAAGC TTCTTATTGA GACCTTGACC AATGGAGATT TCGAACGCAT TCACGTGATC 
AACCGAGCTC AGCTGATCGA CGATGCCCTA TATCTGGCCT GGACCGGCGA ACAGGACTAC 
GAGATCGCCA TGAGACTGAT CGAGTATCTG CAGCGGGAGC GGGAGTATTT GCCCTGGAAG 
TCGGCCTTCG AGAATCTGAA GCGTGTGGGC CGCATTGTCC GCCAGACACC CGATTTCGAG 
TTCTTCAAGC GCTACATGAA GAAGCTGATT CTGCCGATCT ACGAGCACCT GAATGGCATC 
AACGACACAT TCAGCGCGAT TCCGCAGCAG GATCAGGTGC TGCTGAAGAC AATGGTGGTC 
AACTGGGCGT GTCAGTACCA GGTGGGCGAT TGTGTGCCCC AGGCCCTGGC CTACTACCGC 
AACTGGCGAG CGGAGGCTAA TCCCGACGAG AAGAATCCGG TTCCGATCAA CGTTCGTAGC 
ACTGTGTACT GCACCTCCAT AAAGCACGGA TCCGATTCGG ATTGGGAATT CCTGTGGACG 
CGTTACAAGA AGTCGAATGT GGCGGCTGAG AAGCGCACCA TTCTGACTGC TTTGGGTTGT 
TCGAGGGAAG TGTGGCTGCT GCAGCGTTAT CTGGAGCTGA CCTTTGACCC CAAGGAGGCC 
ATTCGCAAGC AGGACTCGAT GTGGGCCTTC CAAGCGGTGG CCTTCAATGA GGTGGGCTTC 
CTGCTGGCCA AAAAGTATTT CATGGACAAC GTCGACTTCA TCTACAAGTT CTACCATCCG 
CTCACCAAGG ACATGTCCCG CCTACTCTCA CCGCTTTCCG AGCAGGTCAT CACGCTGAGC 
GACTTCAACG AGTTCAAGGA CTTCGTGAAC AACTCCCGTC AGTCGCTGAA GGGATTGGAA 
CAGGCCATCC AACAGACACT GGAGATCATG CTCACCAATG TTCAGTGGAA GGAGCGAAAC 
TACCATCAGA TGTCCCGCTC CATCCAGCAA CTGCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287580.1
CDS	54..2852
Translation	MKWFLLFVAALGLGLATADSSADSIETTYNYYRLPTSLRPQKYHLRILTLLENPEDLRFSGSVKILIEALENTKNVTLHSKNLTIDESQITLRQIGGEGKKENCVSSTAVNPSHDFYILNTCQELLAGNTYELYMPFAADLNRQLEGYYRSSYKDPVANLTKWISVTQFEPASARLAFPCFDEPDFKAPFVVTLGYHKKYTAISNMPEKETKPHETLADYIWCEFQESVPMSTYLVAYSVNDFSHKPSTLPNSALFRTWARPNAIDQCDYAAQFGPKVLQYYEQFFGIKFPLPKIDQIAVPDFSAGAMENWGLVTYREIALLYSAAHSSLADKQRVASVVAHELAHQWFGNLVTMKWWTDLWLNEGFATYVASLGVENINPEWRSMEQESLSNLLTIFRRDALESSHPISRPIQMVSEISESFDQISYQKGSTVLRMMHLFLGEESFRSGLQAYLQKFSYKNAEQDNLWESLTQAAHKYRSLPKSYDIKSIMDSWTLQTGYPVINVTRDYAARTAKLNQERYLLNTQVARAYRGGCWWVPLSYTTQAVQDFNNTAPKAWMECGKNGESLPKTIQDLPGPDQWVIFNTQLSTLYKVNYDAQNWKLLIETLTNGDFERIHVINRAQLIDDALYLAWTGEQDYEIAMRLIEYLQREREYLPWKSAFENLKRVGRIVRQTPDFEFFKRYMKKLILPIYEHLNGINDTFSAIPQQDQVLLKTMVVNWACQYQVGDCVPQALAYYRNWRAEANPDEKNPVPINVRSTVYCTSIKHGSDSDWEFLWTRYKKSNVAAEKRTILTALGCSREVWLLQRYLELTFDPKEAIRKQDSMWAFQAVAFNEVGFLLAKKYFMDNVDFIYKFYHPLTKDMSRLLSPLSEQVITLSDFNEFKDFVNNSRQSLKGLEQAIQQTLEIMLTNVQWKERNYHQMSRSIQQLL

Target ORF information:

RefSeq Version	NM_001300651.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster SP1029, transcript variant E (SP1029), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300651.1

ORF Insert Sequence:

ATGAAGTGGT TTCTGCTGTT CGTGGCGGCC CTTGGCCTGG GATTAGCCAC CGCCGACTCA 
TCCGCCGACT CCATCGAGAC CACCTACAAC TACTACCGCC TGCCCACATC GCTGCGCCCG 
CAGAAGTACC ATCTCCGCAT TCTGACACTG CTGGAGAATC CGGAGGACCT GCGTTTTTCC 
GGTTCCGTAA AGATCCTGAT CGAAGCCCTG GAAAACACCA AGAACGTAAC GCTGCACTCC 
AAGAATCTGA CCATCGATGA GTCCCAGATC ACCCTGCGCC AGATCGGCGG AGAGGGCAAG 
AAGGAGAACT GCGTGTCCTC CACGGCGGTG AATCCATCCC ATGACTTCTA CATCCTCAAC 
ACCTGCCAGG AACTTTTGGC CGGCAACACC TACGAGTTGT ACATGCCCTT CGCCGCCGAC 
CTGAACCGAC AACTTGAGGG CTATTACCGC AGCTCCTACA AGGATCCCGT GGCCAATCTT 
ACCAAATGGA TCTCGGTTAC CCAGTTTGAA CCGGCATCGG CTCGATTGGC CTTCCCCTGC 
TTTGATGAGC CCGATTTCAA GGCTCCTTTC GTGGTTACCC TTGGATATCA TAAGAAGTAT 
ACGGCTATAA GCAACATGCC CGAGAAGGAA ACCAAGCCAC ATGAAACCCT CGCCGACTAC 
ATATGGTGCG AGTTCCAGGA GTCGGTGCCC ATGTCCACCT ACCTGGTTGC CTACTCCGTG 
AACGATTTCT CGCACAAGCC CTCCACCCTG CCAAATAGCG CTCTATTCCG AACTTGGGCC 
AGACCCAATG CCATCGATCA GTGCGACTAT GCCGCTCAGT TCGGACCCAA AGTGTTGCAA 
TACTATGAGC AGTTCTTCGG CATCAAGTTC CCGCTACCCA AGATCGATCA GATTGCAGTG 
CCCGATTTCA GTGCCGGCGC CATGGAGAAC TGGGGTCTGG TGACCTACAG GGAGATTGCC 
CTGCTCTACT CGGCGGCACA CTCCTCGCTG GCGGATAAGC AACGAGTGGC CAGCGTGGTG 
GCTCATGAGT TGGCGCACCA GTGGTTTGGT AACCTGGTCA CCATGAAGTG GTGGACCGAC 
CTGTGGCTGA ACGAGGGATT CGCCACCTAT GTGGCCAGTT TGGGTGTGGA GAATATCAAT 
CCGGAATGGC GTTCCATGGA GCAGGAGTCG CTGTCCAATC TGCTGACCAT TTTCCGCAGA 
GACGCCCTGG AGAGCAGCCA TCCCATCTCG AGACCCATCC AAATGGTGTC GGAGATCTCG 
GAGAGCTTCG ATCAGATCTC ATACCAGAAG GGCTCCACGG TGCTGCGCAT GATGCACTTG 
TTCCTGGGCG AGGAGTCCTT CCGCTCCGGT CTGCAGGCCT ATCTGCAGAA GTTCTCCTAC 
AAGAATGCTG AGCAGGACAA TCTCTGGGAG TCGCTCACCC AGGCAGCTCA CAAGTATCGA 
TCTCTGCCCA AGAGCTATGA CATCAAGAGC ATCATGGACT CATGGACCCT GCAAACTGGC 
TACCCTGTGA TCAACGTGAC GCGAGACTAC GCTGCCAGGA CGGCCAAGCT CAACCAGGAG 
CGCTATCTCC TGAACACCCA GGTAGCCCGA GCTTATCGCG GCGGTTGCTG GTGGGTGCCA 
CTGAGTTACA CCACACAGGC GGTGCAGGAC TTCAACAACA CGGCACCCAA GGCCTGGATG 
GAGTGCGGCA AGAATGGCGA GAGCCTGCCC AAGACCATTC AGGACCTGCC AGGACCCGAT 
CAGTGGGTGA TCTTTAACAC GCAGCTCTCG ACGCTGTACA AGGTCAACTA CGATGCCCAG 
AACTGGAAGC TTCTTATTGA GACCTTGACC AATGGAGATT TCGAACGCAT TCACGTGATC 
AACCGAGCTC AGCTGATCGA CGATGCCCTA TATCTGGCCT GGACCGGCGA ACAGGACTAC 
GAGATCGCCA TGAGACTGAT CGAGTATCTG CAGCGGGAGC GGGAGTATTT GCCCTGGAAG 
TCGGCCTTCG AGAATCTGAA GCGTGTGGGC CGCATTGTCC GCCAGACACC CGATTTCGAG 
TTCTTCAAGC GCTACATGAA GAAGCTGATT CTGCCGATCT ACGAGCACCT GAATGGCATC 
AACGACACAT TCAGCGCGAT TCCGCAGCAG GATCAGGTGC TGCTGAAGAC AATGGTGGTC 
AACTGGGCGT GTCAGTACCA GGTGGGCGAT TGTGTGCCCC AGGCCCTGGC CTACTACCGC 
AACTGGCGAG CGGAGGCTAA TCCCGACGAG AAGAATCCGG TTCCGATCAA CGTTCGTAGC 
ACTGTGTACT GCACCTCCAT AAAGCACGGA TCCGATTCGG ATTGGGAATT CCTGTGGACG 
CGTTACAAGA AGTCGAATGT GGCGGCTGAG AAGCGCACCA TTCTGACTGC TTTGGGTTGT 
TCGAGGGAAG TGTGGCTGCT GCAGCGTTAT CTGGAGCTGA CCTTTGACCC CAAGGAGGCC 
ATTCGCAAGC AGGACTCGAT GTGGGCCTTC CAAGCGGTGG CCTTCAATGA GGTGGGCTTC 
CTGCTGGCCA AAAAGTATTT CATGGACAAC GTCGACTTCA TCTACAAGTT CTACCATCCG 
CTCACCAAGG ACATGTCCCG CCTACTCTCA CCGCTTTCCG AGCAGGTCAT CACGCTGAGC 
GACTTCAACG AGTTCAAGGA CTTCGTGAAC AACTCCCGTC AGTCGCTGAA GGGATTGGAA 
CAGGCCATCC AACAGACACT GGAGATCATG CTCACCAATG TTCAGTGGAA GGAGCGAAAC 
TACCATCAGA TGTCCCGCTC CATCCAGCAA CTGCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SP1029 ( NM_170406.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733285.1 Drosophila melanogaster SP1029, transcript variant C (SP1029), mRNA.

Price & Availability↑

CloneID	OFa07015
Clone ID Related Accession (Same CDS sequence)	NM_170405.2 , NM_144361.2 , NM_001300651.1 , NM_170406.2 , NM_001276136.1
Accession Version	NM_170406.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2799bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	SP1029, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170406.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGTGGT TTCTGCTGTT CGTGGCGGCC CTTGGCCTGG GATTAGCCAC CGCCGACTCA 
TCCGCCGACT CCATCGAGAC CACCTACAAC TACTACCGCC TGCCCACATC GCTGCGCCCG 
CAGAAGTACC ATCTCCGCAT TCTGACACTG CTGGAGAATC CGGAGGACCT GCGTTTTTCC 
GGTTCCGTAA AGATCCTGAT CGAAGCCCTG GAAAACACCA AGAACGTAAC GCTGCACTCC 
AAGAATCTGA CCATCGATGA GTCCCAGATC ACCCTGCGCC AGATCGGCGG AGAGGGCAAG 
AAGGAGAACT GCGTGTCCTC CACGGCGGTG AATCCATCCC ATGACTTCTA CATCCTCAAC 
ACCTGCCAGG AACTTTTGGC CGGCAACACC TACGAGTTGT ACATGCCCTT CGCCGCCGAC 
CTGAACCGAC AACTTGAGGG CTATTACCGC AGCTCCTACA AGGATCCCGT GGCCAATCTT 
ACCAAATGGA TCTCGGTTAC CCAGTTTGAA CCGGCATCGG CTCGATTGGC CTTCCCCTGC 
TTTGATGAGC CCGATTTCAA GGCTCCTTTC GTGGTTACCC TTGGATATCA TAAGAAGTAT 
ACGGCTATAA GCAACATGCC CGAGAAGGAA ACCAAGCCAC ATGAAACCCT CGCCGACTAC 
ATATGGTGCG AGTTCCAGGA GTCGGTGCCC ATGTCCACCT ACCTGGTTGC CTACTCCGTG 
AACGATTTCT CGCACAAGCC CTCCACCCTG CCAAATAGCG CTCTATTCCG AACTTGGGCC 
AGACCCAATG CCATCGATCA GTGCGACTAT GCCGCTCAGT TCGGACCCAA AGTGTTGCAA 
TACTATGAGC AGTTCTTCGG CATCAAGTTC CCGCTACCCA AGATCGATCA GATTGCAGTG 
CCCGATTTCA GTGCCGGCGC CATGGAGAAC TGGGGTCTGG TGACCTACAG GGAGATTGCC 
CTGCTCTACT CGGCGGCACA CTCCTCGCTG GCGGATAAGC AACGAGTGGC CAGCGTGGTG 
GCTCATGAGT TGGCGCACCA GTGGTTTGGT AACCTGGTCA CCATGAAGTG GTGGACCGAC 
CTGTGGCTGA ACGAGGGATT CGCCACCTAT GTGGCCAGTT TGGGTGTGGA GAATATCAAT 
CCGGAATGGC GTTCCATGGA GCAGGAGTCG CTGTCCAATC TGCTGACCAT TTTCCGCAGA 
GACGCCCTGG AGAGCAGCCA TCCCATCTCG AGACCCATCC AAATGGTGTC GGAGATCTCG 
GAGAGCTTCG ATCAGATCTC ATACCAGAAG GGCTCCACGG TGCTGCGCAT GATGCACTTG 
TTCCTGGGCG AGGAGTCCTT CCGCTCCGGT CTGCAGGCCT ATCTGCAGAA GTTCTCCTAC 
AAGAATGCTG AGCAGGACAA TCTCTGGGAG TCGCTCACCC AGGCAGCTCA CAAGTATCGA 
TCTCTGCCCA AGAGCTATGA CATCAAGAGC ATCATGGACT CATGGACCCT GCAAACTGGC 
TACCCTGTGA TCAACGTGAC GCGAGACTAC GCTGCCAGGA CGGCCAAGCT CAACCAGGAG 
CGCTATCTCC TGAACACCCA GGTAGCCCGA GCTTATCGCG GCGGTTGCTG GTGGGTGCCA 
CTGAGTTACA CCACACAGGC GGTGCAGGAC TTCAACAACA CGGCACCCAA GGCCTGGATG 
GAGTGCGGCA AGAATGGCGA GAGCCTGCCC AAGACCATTC AGGACCTGCC AGGACCCGAT 
CAGTGGGTGA TCTTTAACAC GCAGCTCTCG ACGCTGTACA AGGTCAACTA CGATGCCCAG 
AACTGGAAGC TTCTTATTGA GACCTTGACC AATGGAGATT TCGAACGCAT TCACGTGATC 
AACCGAGCTC AGCTGATCGA CGATGCCCTA TATCTGGCCT GGACCGGCGA ACAGGACTAC 
GAGATCGCCA TGAGACTGAT CGAGTATCTG CAGCGGGAGC GGGAGTATTT GCCCTGGAAG 
TCGGCCTTCG AGAATCTGAA GCGTGTGGGC CGCATTGTCC GCCAGACACC CGATTTCGAG 
TTCTTCAAGC GCTACATGAA GAAGCTGATT CTGCCGATCT ACGAGCACCT GAATGGCATC 
AACGACACAT TCAGCGCGAT TCCGCAGCAG GATCAGGTGC TGCTGAAGAC AATGGTGGTC 
AACTGGGCGT GTCAGTACCA GGTGGGCGAT TGTGTGCCCC AGGCCCTGGC CTACTACCGC 
AACTGGCGAG CGGAGGCTAA TCCCGACGAG AAGAATCCGG TTCCGATCAA CGTTCGTAGC 
ACTGTGTACT GCACCTCCAT AAAGCACGGA TCCGATTCGG ATTGGGAATT CCTGTGGACG 
CGTTACAAGA AGTCGAATGT GGCGGCTGAG AAGCGCACCA TTCTGACTGC TTTGGGTTGT 
TCGAGGGAAG TGTGGCTGCT GCAGCGTTAT CTGGAGCTGA CCTTTGACCC CAAGGAGGCC 
ATTCGCAAGC AGGACTCGAT GTGGGCCTTC CAAGCGGTGG CCTTCAATGA GGTGGGCTTC 
CTGCTGGCCA AAAAGTATTT CATGGACAAC GTCGACTTCA TCTACAAGTT CTACCATCCG 
CTCACCAAGG ACATGTCCCG CCTACTCTCA CCGCTTTCCG AGCAGGTCAT CACGCTGAGC 
GACTTCAACG AGTTCAAGGA CTTCGTGAAC AACTCCCGTC AGTCGCTGAA GGGATTGGAA 
CAGGCCATCC AACAGACACT GGAGATCATG CTCACCAATG TTCAGTGGAA GGAGCGAAAC 
TACCATCAGA TGTCCCGCTC CATCCAGCAA CTGCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733285.1
CDS	231..3029
Translation	MKWFLLFVAALGLGLATADSSADSIETTYNYYRLPTSLRPQKYHLRILTLLENPEDLRFSGSVKILIEALENTKNVTLHSKNLTIDESQITLRQIGGEGKKENCVSSTAVNPSHDFYILNTCQELLAGNTYELYMPFAADLNRQLEGYYRSSYKDPVANLTKWISVTQFEPASARLAFPCFDEPDFKAPFVVTLGYHKKYTAISNMPEKETKPHETLADYIWCEFQESVPMSTYLVAYSVNDFSHKPSTLPNSALFRTWARPNAIDQCDYAAQFGPKVLQYYEQFFGIKFPLPKIDQIAVPDFSAGAMENWGLVTYREIALLYSAAHSSLADKQRVASVVAHELAHQWFGNLVTMKWWTDLWLNEGFATYVASLGVENINPEWRSMEQESLSNLLTIFRRDALESSHPISRPIQMVSEISESFDQISYQKGSTVLRMMHLFLGEESFRSGLQAYLQKFSYKNAEQDNLWESLTQAAHKYRSLPKSYDIKSIMDSWTLQTGYPVINVTRDYAARTAKLNQERYLLNTQVARAYRGGCWWVPLSYTTQAVQDFNNTAPKAWMECGKNGESLPKTIQDLPGPDQWVIFNTQLSTLYKVNYDAQNWKLLIETLTNGDFERIHVINRAQLIDDALYLAWTGEQDYEIAMRLIEYLQREREYLPWKSAFENLKRVGRIVRQTPDFEFFKRYMKKLILPIYEHLNGINDTFSAIPQQDQVLLKTMVVNWACQYQVGDCVPQALAYYRNWRAEANPDEKNPVPINVRSTVYCTSIKHGSDSDWEFLWTRYKKSNVAAEKRTILTALGCSREVWLLQRYLELTFDPKEAIRKQDSMWAFQAVAFNEVGFLLAKKYFMDNVDFIYKFYHPLTKDMSRLLSPLSEQVITLSDFNEFKDFVNNSRQSLKGLEQAIQQTLEIMLTNVQWKERNYHQMSRSIQQLL

Target ORF information:

RefSeq Version	NM_170406.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster SP1029, transcript variant C (SP1029), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170406.2

ORF Insert Sequence:

ATGAAGTGGT TTCTGCTGTT CGTGGCGGCC CTTGGCCTGG GATTAGCCAC CGCCGACTCA 
TCCGCCGACT CCATCGAGAC CACCTACAAC TACTACCGCC TGCCCACATC GCTGCGCCCG 
CAGAAGTACC ATCTCCGCAT TCTGACACTG CTGGAGAATC CGGAGGACCT GCGTTTTTCC 
GGTTCCGTAA AGATCCTGAT CGAAGCCCTG GAAAACACCA AGAACGTAAC GCTGCACTCC 
AAGAATCTGA CCATCGATGA GTCCCAGATC ACCCTGCGCC AGATCGGCGG AGAGGGCAAG 
AAGGAGAACT GCGTGTCCTC CACGGCGGTG AATCCATCCC ATGACTTCTA CATCCTCAAC 
ACCTGCCAGG AACTTTTGGC CGGCAACACC TACGAGTTGT ACATGCCCTT CGCCGCCGAC 
CTGAACCGAC AACTTGAGGG CTATTACCGC AGCTCCTACA AGGATCCCGT GGCCAATCTT 
ACCAAATGGA TCTCGGTTAC CCAGTTTGAA CCGGCATCGG CTCGATTGGC CTTCCCCTGC 
TTTGATGAGC CCGATTTCAA GGCTCCTTTC GTGGTTACCC TTGGATATCA TAAGAAGTAT 
ACGGCTATAA GCAACATGCC CGAGAAGGAA ACCAAGCCAC ATGAAACCCT CGCCGACTAC 
ATATGGTGCG AGTTCCAGGA GTCGGTGCCC ATGTCCACCT ACCTGGTTGC CTACTCCGTG 
AACGATTTCT CGCACAAGCC CTCCACCCTG CCAAATAGCG CTCTATTCCG AACTTGGGCC 
AGACCCAATG CCATCGATCA GTGCGACTAT GCCGCTCAGT TCGGACCCAA AGTGTTGCAA 
TACTATGAGC AGTTCTTCGG CATCAAGTTC CCGCTACCCA AGATCGATCA GATTGCAGTG 
CCCGATTTCA GTGCCGGCGC CATGGAGAAC TGGGGTCTGG TGACCTACAG GGAGATTGCC 
CTGCTCTACT CGGCGGCACA CTCCTCGCTG GCGGATAAGC AACGAGTGGC CAGCGTGGTG 
GCTCATGAGT TGGCGCACCA GTGGTTTGGT AACCTGGTCA CCATGAAGTG GTGGACCGAC 
CTGTGGCTGA ACGAGGGATT CGCCACCTAT GTGGCCAGTT TGGGTGTGGA GAATATCAAT 
CCGGAATGGC GTTCCATGGA GCAGGAGTCG CTGTCCAATC TGCTGACCAT TTTCCGCAGA 
GACGCCCTGG AGAGCAGCCA TCCCATCTCG AGACCCATCC AAATGGTGTC GGAGATCTCG 
GAGAGCTTCG ATCAGATCTC ATACCAGAAG GGCTCCACGG TGCTGCGCAT GATGCACTTG 
TTCCTGGGCG AGGAGTCCTT CCGCTCCGGT CTGCAGGCCT ATCTGCAGAA GTTCTCCTAC 
AAGAATGCTG AGCAGGACAA TCTCTGGGAG TCGCTCACCC AGGCAGCTCA CAAGTATCGA 
TCTCTGCCCA AGAGCTATGA CATCAAGAGC ATCATGGACT CATGGACCCT GCAAACTGGC 
TACCCTGTGA TCAACGTGAC GCGAGACTAC GCTGCCAGGA CGGCCAAGCT CAACCAGGAG 
CGCTATCTCC TGAACACCCA GGTAGCCCGA GCTTATCGCG GCGGTTGCTG GTGGGTGCCA 
CTGAGTTACA CCACACAGGC GGTGCAGGAC TTCAACAACA CGGCACCCAA GGCCTGGATG 
GAGTGCGGCA AGAATGGCGA GAGCCTGCCC AAGACCATTC AGGACCTGCC AGGACCCGAT 
CAGTGGGTGA TCTTTAACAC GCAGCTCTCG ACGCTGTACA AGGTCAACTA CGATGCCCAG 
AACTGGAAGC TTCTTATTGA GACCTTGACC AATGGAGATT TCGAACGCAT TCACGTGATC 
AACCGAGCTC AGCTGATCGA CGATGCCCTA TATCTGGCCT GGACCGGCGA ACAGGACTAC 
GAGATCGCCA TGAGACTGAT CGAGTATCTG CAGCGGGAGC GGGAGTATTT GCCCTGGAAG 
TCGGCCTTCG AGAATCTGAA GCGTGTGGGC CGCATTGTCC GCCAGACACC CGATTTCGAG 
TTCTTCAAGC GCTACATGAA GAAGCTGATT CTGCCGATCT ACGAGCACCT GAATGGCATC 
AACGACACAT TCAGCGCGAT TCCGCAGCAG GATCAGGTGC TGCTGAAGAC AATGGTGGTC 
AACTGGGCGT GTCAGTACCA GGTGGGCGAT TGTGTGCCCC AGGCCCTGGC CTACTACCGC 
AACTGGCGAG CGGAGGCTAA TCCCGACGAG AAGAATCCGG TTCCGATCAA CGTTCGTAGC 
ACTGTGTACT GCACCTCCAT AAAGCACGGA TCCGATTCGG ATTGGGAATT CCTGTGGACG 
CGTTACAAGA AGTCGAATGT GGCGGCTGAG AAGCGCACCA TTCTGACTGC TTTGGGTTGT 
TCGAGGGAAG TGTGGCTGCT GCAGCGTTAT CTGGAGCTGA CCTTTGACCC CAAGGAGGCC 
ATTCGCAAGC AGGACTCGAT GTGGGCCTTC CAAGCGGTGG CCTTCAATGA GGTGGGCTTC 
CTGCTGGCCA AAAAGTATTT CATGGACAAC GTCGACTTCA TCTACAAGTT CTACCATCCG 
CTCACCAAGG ACATGTCCCG CCTACTCTCA CCGCTTTCCG AGCAGGTCAT CACGCTGAGC 
GACTTCAACG AGTTCAAGGA CTTCGTGAAC AACTCCCGTC AGTCGCTGAA GGGATTGGAA 
CAGGCCATCC AACAGACACT GGAGATCATG CTCACCAATG TTCAGTGGAA GGAGCGAAAC 
TACCATCAGA TGTCCCGCTC CATCCAGCAA CTGCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SP1029 ( NM_001276136.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001263065.1 Drosophila melanogaster SP1029, transcript variant D (SP1029), mRNA.

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CloneID	OFa07015
Clone ID Related Accession (Same CDS sequence)	NM_170405.2 , NM_144361.2 , NM_001300651.1 , NM_170406.2 , NM_001276136.1
Accession Version	NM_001276136.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2799bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	SP1029, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGTGGT TTCTGCTGTT CGTGGCGGCC CTTGGCCTGG GATTAGCCAC CGCCGACTCA 
TCCGCCGACT CCATCGAGAC CACCTACAAC TACTACCGCC TGCCCACATC GCTGCGCCCG 
CAGAAGTACC ATCTCCGCAT TCTGACACTG CTGGAGAATC CGGAGGACCT GCGTTTTTCC 
GGTTCCGTAA AGATCCTGAT CGAAGCCCTG GAAAACACCA AGAACGTAAC GCTGCACTCC 
AAGAATCTGA CCATCGATGA GTCCCAGATC ACCCTGCGCC AGATCGGCGG AGAGGGCAAG 
AAGGAGAACT GCGTGTCCTC CACGGCGGTG AATCCATCCC ATGACTTCTA CATCCTCAAC 
ACCTGCCAGG AACTTTTGGC CGGCAACACC TACGAGTTGT ACATGCCCTT CGCCGCCGAC 
CTGAACCGAC AACTTGAGGG CTATTACCGC AGCTCCTACA AGGATCCCGT GGCCAATCTT 
ACCAAATGGA TCTCGGTTAC CCAGTTTGAA CCGGCATCGG CTCGATTGGC CTTCCCCTGC 
TTTGATGAGC CCGATTTCAA GGCTCCTTTC GTGGTTACCC TTGGATATCA TAAGAAGTAT 
ACGGCTATAA GCAACATGCC CGAGAAGGAA ACCAAGCCAC ATGAAACCCT CGCCGACTAC 
ATATGGTGCG AGTTCCAGGA GTCGGTGCCC ATGTCCACCT ACCTGGTTGC CTACTCCGTG 
AACGATTTCT CGCACAAGCC CTCCACCCTG CCAAATAGCG CTCTATTCCG AACTTGGGCC 
AGACCCAATG CCATCGATCA GTGCGACTAT GCCGCTCAGT TCGGACCCAA AGTGTTGCAA 
TACTATGAGC AGTTCTTCGG CATCAAGTTC CCGCTACCCA AGATCGATCA GATTGCAGTG 
CCCGATTTCA GTGCCGGCGC CATGGAGAAC TGGGGTCTGG TGACCTACAG GGAGATTGCC 
CTGCTCTACT CGGCGGCACA CTCCTCGCTG GCGGATAAGC AACGAGTGGC CAGCGTGGTG 
GCTCATGAGT TGGCGCACCA GTGGTTTGGT AACCTGGTCA CCATGAAGTG GTGGACCGAC 
CTGTGGCTGA ACGAGGGATT CGCCACCTAT GTGGCCAGTT TGGGTGTGGA GAATATCAAT 
CCGGAATGGC GTTCCATGGA GCAGGAGTCG CTGTCCAATC TGCTGACCAT TTTCCGCAGA 
GACGCCCTGG AGAGCAGCCA TCCCATCTCG AGACCCATCC AAATGGTGTC GGAGATCTCG 
GAGAGCTTCG ATCAGATCTC ATACCAGAAG GGCTCCACGG TGCTGCGCAT GATGCACTTG 
TTCCTGGGCG AGGAGTCCTT CCGCTCCGGT CTGCAGGCCT ATCTGCAGAA GTTCTCCTAC 
AAGAATGCTG AGCAGGACAA TCTCTGGGAG TCGCTCACCC AGGCAGCTCA CAAGTATCGA 
TCTCTGCCCA AGAGCTATGA CATCAAGAGC ATCATGGACT CATGGACCCT GCAAACTGGC 
TACCCTGTGA TCAACGTGAC GCGAGACTAC GCTGCCAGGA CGGCCAAGCT CAACCAGGAG 
CGCTATCTCC TGAACACCCA GGTAGCCCGA GCTTATCGCG GCGGTTGCTG GTGGGTGCCA 
CTGAGTTACA CCACACAGGC GGTGCAGGAC TTCAACAACA CGGCACCCAA GGCCTGGATG 
GAGTGCGGCA AGAATGGCGA GAGCCTGCCC AAGACCATTC AGGACCTGCC AGGACCCGAT 
CAGTGGGTGA TCTTTAACAC GCAGCTCTCG ACGCTGTACA AGGTCAACTA CGATGCCCAG 
AACTGGAAGC TTCTTATTGA GACCTTGACC AATGGAGATT TCGAACGCAT TCACGTGATC 
AACCGAGCTC AGCTGATCGA CGATGCCCTA TATCTGGCCT GGACCGGCGA ACAGGACTAC 
GAGATCGCCA TGAGACTGAT CGAGTATCTG CAGCGGGAGC GGGAGTATTT GCCCTGGAAG 
TCGGCCTTCG AGAATCTGAA GCGTGTGGGC CGCATTGTCC GCCAGACACC CGATTTCGAG 
TTCTTCAAGC GCTACATGAA GAAGCTGATT CTGCCGATCT ACGAGCACCT GAATGGCATC 
AACGACACAT TCAGCGCGAT TCCGCAGCAG GATCAGGTGC TGCTGAAGAC AATGGTGGTC 
AACTGGGCGT GTCAGTACCA GGTGGGCGAT TGTGTGCCCC AGGCCCTGGC CTACTACCGC 
AACTGGCGAG CGGAGGCTAA TCCCGACGAG AAGAATCCGG TTCCGATCAA CGTTCGTAGC 
ACTGTGTACT GCACCTCCAT AAAGCACGGA TCCGATTCGG ATTGGGAATT CCTGTGGACG 
CGTTACAAGA AGTCGAATGT GGCGGCTGAG AAGCGCACCA TTCTGACTGC TTTGGGTTGT 
TCGAGGGAAG TGTGGCTGCT GCAGCGTTAT CTGGAGCTGA CCTTTGACCC CAAGGAGGCC 
ATTCGCAAGC AGGACTCGAT GTGGGCCTTC CAAGCGGTGG CCTTCAATGA GGTGGGCTTC 
CTGCTGGCCA AAAAGTATTT CATGGACAAC GTCGACTTCA TCTACAAGTT CTACCATCCG 
CTCACCAAGG ACATGTCCCG CCTACTCTCA CCGCTTTCCG AGCAGGTCAT CACGCTGAGC 
GACTTCAACG AGTTCAAGGA CTTCGTGAAC AACTCCCGTC AGTCGCTGAA GGGATTGGAA 
CAGGCCATCC AACAGACACT GGAGATCATG CTCACCAATG TTCAGTGGAA GGAGCGAAAC 
TACCATCAGA TGTCCCGCTC CATCCAGCAA CTGCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263065.1
CDS	271..3069
Translation	MKWFLLFVAALGLGLATADSSADSIETTYNYYRLPTSLRPQKYHLRILTLLENPEDLRFSGSVKILIEALENTKNVTLHSKNLTIDESQITLRQIGGEGKKENCVSSTAVNPSHDFYILNTCQELLAGNTYELYMPFAADLNRQLEGYYRSSYKDPVANLTKWISVTQFEPASARLAFPCFDEPDFKAPFVVTLGYHKKYTAISNMPEKETKPHETLADYIWCEFQESVPMSTYLVAYSVNDFSHKPSTLPNSALFRTWARPNAIDQCDYAAQFGPKVLQYYEQFFGIKFPLPKIDQIAVPDFSAGAMENWGLVTYREIALLYSAAHSSLADKQRVASVVAHELAHQWFGNLVTMKWWTDLWLNEGFATYVASLGVENINPEWRSMEQESLSNLLTIFRRDALESSHPISRPIQMVSEISESFDQISYQKGSTVLRMMHLFLGEESFRSGLQAYLQKFSYKNAEQDNLWESLTQAAHKYRSLPKSYDIKSIMDSWTLQTGYPVINVTRDYAARTAKLNQERYLLNTQVARAYRGGCWWVPLSYTTQAVQDFNNTAPKAWMECGKNGESLPKTIQDLPGPDQWVIFNTQLSTLYKVNYDAQNWKLLIETLTNGDFERIHVINRAQLIDDALYLAWTGEQDYEIAMRLIEYLQREREYLPWKSAFENLKRVGRIVRQTPDFEFFKRYMKKLILPIYEHLNGINDTFSAIPQQDQVLLKTMVVNWACQYQVGDCVPQALAYYRNWRAEANPDEKNPVPINVRSTVYCTSIKHGSDSDWEFLWTRYKKSNVAAEKRTILTALGCSREVWLLQRYLELTFDPKEAIRKQDSMWAFQAVAFNEVGFLLAKKYFMDNVDFIYKFYHPLTKDMSRLLSPLSEQVITLSDFNEFKDFVNNSRQSLKGLEQAIQQTLEIMLTNVQWKERNYHQMSRSIQQLL

Target ORF information:

RefSeq Version	NM_001276136.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster SP1029, transcript variant D (SP1029), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276136.1

ORF Insert Sequence:

ATGAAGTGGT TTCTGCTGTT CGTGGCGGCC CTTGGCCTGG GATTAGCCAC CGCCGACTCA 
TCCGCCGACT CCATCGAGAC CACCTACAAC TACTACCGCC TGCCCACATC GCTGCGCCCG 
CAGAAGTACC ATCTCCGCAT TCTGACACTG CTGGAGAATC CGGAGGACCT GCGTTTTTCC 
GGTTCCGTAA AGATCCTGAT CGAAGCCCTG GAAAACACCA AGAACGTAAC GCTGCACTCC 
AAGAATCTGA CCATCGATGA GTCCCAGATC ACCCTGCGCC AGATCGGCGG AGAGGGCAAG 
AAGGAGAACT GCGTGTCCTC CACGGCGGTG AATCCATCCC ATGACTTCTA CATCCTCAAC 
ACCTGCCAGG AACTTTTGGC CGGCAACACC TACGAGTTGT ACATGCCCTT CGCCGCCGAC 
CTGAACCGAC AACTTGAGGG CTATTACCGC AGCTCCTACA AGGATCCCGT GGCCAATCTT 
ACCAAATGGA TCTCGGTTAC CCAGTTTGAA CCGGCATCGG CTCGATTGGC CTTCCCCTGC 
TTTGATGAGC CCGATTTCAA GGCTCCTTTC GTGGTTACCC TTGGATATCA TAAGAAGTAT 
ACGGCTATAA GCAACATGCC CGAGAAGGAA ACCAAGCCAC ATGAAACCCT CGCCGACTAC 
ATATGGTGCG AGTTCCAGGA GTCGGTGCCC ATGTCCACCT ACCTGGTTGC CTACTCCGTG 
AACGATTTCT CGCACAAGCC CTCCACCCTG CCAAATAGCG CTCTATTCCG AACTTGGGCC 
AGACCCAATG CCATCGATCA GTGCGACTAT GCCGCTCAGT TCGGACCCAA AGTGTTGCAA 
TACTATGAGC AGTTCTTCGG CATCAAGTTC CCGCTACCCA AGATCGATCA GATTGCAGTG 
CCCGATTTCA GTGCCGGCGC CATGGAGAAC TGGGGTCTGG TGACCTACAG GGAGATTGCC 
CTGCTCTACT CGGCGGCACA CTCCTCGCTG GCGGATAAGC AACGAGTGGC CAGCGTGGTG 
GCTCATGAGT TGGCGCACCA GTGGTTTGGT AACCTGGTCA CCATGAAGTG GTGGACCGAC 
CTGTGGCTGA ACGAGGGATT CGCCACCTAT GTGGCCAGTT TGGGTGTGGA GAATATCAAT 
CCGGAATGGC GTTCCATGGA GCAGGAGTCG CTGTCCAATC TGCTGACCAT TTTCCGCAGA 
GACGCCCTGG AGAGCAGCCA TCCCATCTCG AGACCCATCC AAATGGTGTC GGAGATCTCG 
GAGAGCTTCG ATCAGATCTC ATACCAGAAG GGCTCCACGG TGCTGCGCAT GATGCACTTG 
TTCCTGGGCG AGGAGTCCTT CCGCTCCGGT CTGCAGGCCT ATCTGCAGAA GTTCTCCTAC 
AAGAATGCTG AGCAGGACAA TCTCTGGGAG TCGCTCACCC AGGCAGCTCA CAAGTATCGA 
TCTCTGCCCA AGAGCTATGA CATCAAGAGC ATCATGGACT CATGGACCCT GCAAACTGGC 
TACCCTGTGA TCAACGTGAC GCGAGACTAC GCTGCCAGGA CGGCCAAGCT CAACCAGGAG 
CGCTATCTCC TGAACACCCA GGTAGCCCGA GCTTATCGCG GCGGTTGCTG GTGGGTGCCA 
CTGAGTTACA CCACACAGGC GGTGCAGGAC TTCAACAACA CGGCACCCAA GGCCTGGATG 
GAGTGCGGCA AGAATGGCGA GAGCCTGCCC AAGACCATTC AGGACCTGCC AGGACCCGAT 
CAGTGGGTGA TCTTTAACAC GCAGCTCTCG ACGCTGTACA AGGTCAACTA CGATGCCCAG 
AACTGGAAGC TTCTTATTGA GACCTTGACC AATGGAGATT TCGAACGCAT TCACGTGATC 
AACCGAGCTC AGCTGATCGA CGATGCCCTA TATCTGGCCT GGACCGGCGA ACAGGACTAC 
GAGATCGCCA TGAGACTGAT CGAGTATCTG CAGCGGGAGC GGGAGTATTT GCCCTGGAAG 
TCGGCCTTCG AGAATCTGAA GCGTGTGGGC CGCATTGTCC GCCAGACACC CGATTTCGAG 
TTCTTCAAGC GCTACATGAA GAAGCTGATT CTGCCGATCT ACGAGCACCT GAATGGCATC 
AACGACACAT TCAGCGCGAT TCCGCAGCAG GATCAGGTGC TGCTGAAGAC AATGGTGGTC 
AACTGGGCGT GTCAGTACCA GGTGGGCGAT TGTGTGCCCC AGGCCCTGGC CTACTACCGC 
AACTGGCGAG CGGAGGCTAA TCCCGACGAG AAGAATCCGG TTCCGATCAA CGTTCGTAGC 
ACTGTGTACT GCACCTCCAT AAAGCACGGA TCCGATTCGG ATTGGGAATT CCTGTGGACG 
CGTTACAAGA AGTCGAATGT GGCGGCTGAG AAGCGCACCA TTCTGACTGC TTTGGGTTGT 
TCGAGGGAAG TGTGGCTGCT GCAGCGTTAT CTGGAGCTGA CCTTTGACCC CAAGGAGGCC 
ATTCGCAAGC AGGACTCGAT GTGGGCCTTC CAAGCGGTGG CCTTCAATGA GGTGGGCTTC 
CTGCTGGCCA AAAAGTATTT CATGGACAAC GTCGACTTCA TCTACAAGTT CTACCATCCG 
CTCACCAAGG ACATGTCCCG CCTACTCTCA CCGCTTTCCG AGCAGGTCAT CACGCTGAGC 
GACTTCAACG AGTTCAAGGA CTTCGTGAAC AACTCCCGTC AGTCGCTGAA GGGATTGGAA 
CAGGCCATCC AACAGACACT GGAGATCATG CTCACCAATG TTCAGTGGAA GGAGCGAAAC 
TACCATCAGA TGTCCCGCTC CATCCAGCAA CTGCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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SP1029 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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