fw cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	fw
Entrez Gene ID	32162
Full Name	furrowed
Synonyms	CG1500,CT3745,Dmel\CG1500,fw-like,wr
General protein information	Preferred Names furrowed Names CG1500 gene product from transcript CG1500-RA CG1500-PA CG1500-PB CG1500-PC fw-PA fw-PB fw-PC wrinkled
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 11A1-11A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001298217.1	NP_001285146.1	furrowed, isoform C
NM_078581.3	NP_511136.2	furrowed, isoform A
NM_001103491.1	NP_001096961.1	furrowed, isoform B

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	CTLSE1	XP_309111.3
Xenopus tropicalis (tropical clawed frog)	LOC101731899	XP_004915691.1
Drosophila melanogaster (fruit fly)	fw	NP_511136.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our data suggest that Fw acts as a cell-adhesion molecule, participating in PCP by mediating the interaction between Fz and Vang/Stbm during early pupal wing and thorax development.
Title: The Drosophila selectin furrowed mediates intercellular planar cell polarity interactions via frizzled stabilization.

The following fw gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fw cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01364	NM_001103491.1 Latest version!	Drosophila melanogaster furrowed (fw), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa01363	NM_001298217.1 Latest version!	Drosophila melanogaster furrowed (fw), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa01363	NM_078581.3 Latest version!	Drosophila melanogaster furrowed (fw), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

fw ( NM_001103491.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001096961.1 Drosophila melanogaster furrowed (fw), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa01364
Clone ID Related Accession (Same CDS sequence)	NM_001103491.1
Accession Version	NM_001103491.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	furrowed, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCAAAA AGTTCACTCT GGGCGAGAAG ATTCAGTACA CCTGTCCCAA GGGCCATTCG 
CTGCTCGGTC AGACGGAACG GGAGTGTAGA CTTGATGGCA CTTGGAGTGG CTCCTCGCCG 
ACCTGCAAAT ATGTGGACTG CGGCAGTCTG CCGGAGCTGA AGTTTGGATC CATCCACATG 
TCGGAGGAGC GGACTAGCTT CGGCGTGGTG GCCACATACA GTTGCCACGA GAACTACACG 
CTAATCGGGA ATGAGAATCG CACGTGCGCC ATGGACGGCT GGAGCGGCAA GCAGCCGGAA 
TGCCTGGTGG ACTGGTGTCC AGATCCGCAG CCCATAGCCG GTGGCGATGT GCGATTCAAT 
GACAAGCGTG CCGGTTCCAC GGCCACGTAT GTTTGTGAGC CGGGCTATGT GCTAGTTGGC 
GAGGCGATCA TCTCGTGCGG TTTGGGCGGC GAGTGGTCCA GCAAGACGCC CTCGTGCAGA 
TTCGTCGATT GTGGTGCACC GGCACGACCA AATCGTGGCA TTGCCATCCT GCTGAACGGC 
ACCACCACCG TCAATTCGGT GGTTAAATAC GAGTGCGACG AGGATCATTG GCTGGACGGG 
CAGTCGGAGC TCTATTGCAC CCGGGAAGGG AAGTGGTCGG GTGAGGCGCC CGTCTGTGAA 
CTGGTCACCT GCGAGACGCC ATCGGTGCCA TCCGGTTCGT TTGTCATTGG CTACGATTAC 
AATGTCCATT CGAAGATCCA GTACAACTGC GATCCCGGTC ACATTATGCA CGGCACTCCG 
GTGCTCGAGT GCCTGGACTC CGGGGAATGG AGTGCCGATG CGCCCTACTG CGAATATATC 
GACTGCGGCA CAATCCTGCC CATTCCCTAT GGCAGCCACA AGTACGTGAC GAATACCACC 
TATGTGGGCT CCGAAGTGGT CTTCAGCTGC TCCCAATCGC ACAAACTGAG CGGAGTCCTC 
AAGCGCACGT GCCTGGAATC GGCTGTCTGG AGCGATGCGT CGCCCAAGTG CGAGGAGATT 
CGCTGTCCGG AACCGAAACT GGCTGCGCAC AGTCTGCTCT CCGTCACCGG AAATGATCGC 
ATGTATGGAA GGACACTTAT CCGCACCTCC GAATCCTCGC AGAACACAGC TCAAACCTAT 
AAGATTGGAG CACTGGCCAA GTACCGATGC GAACGGGGCT ACAAGATGGT GGGCGAGGCA 
CTGGCCACCT GCACGGATAG TGGACAATGG AGCGGCACCA TTCCGGAATG TGTGTATGTG 
GAGTGCGGTG CTCCGGAGGG CATCAACAAT GGCAAGGTGG TCCTGGCCAC AAATGCCACG 
TACTACGGCG CCGCTGTCCT CTATGAGTGC AATGTGAACT TCAAGCTAAA CGGAGTATCC 
CGACGCCTGT GCACGGAGCA CGGCAACTGG AGCCACGAGG CACCGGAATG CGTGGAGGTG 
GTCTGCGATA CGCCCAACAT CAATGAGAAT CTGATCGTGG AGGCGGGTCC GCGTGCTGTG 
GGCTCCGTGG CCACCTTCAA GTGCGCCAAG GGCAGGATCA TGATGGGAAA CGATACGCGT 
GTGTGTCAGA AAAATGGCAA ATGGACTGGC AAGAGTCCCA CCTGCAGACC CGTCGACTGT 
GGTCGTCCTC TGGCCATCGA GAATGGTCGC GTGATTGTGG TCAACGATTC CACGTTGTAC 
GGCGGATCGG CGGAGTATCA CTGCATACCG AACTACAATC GGATTGGCCA GTATTTGCGC 
AAGTGCACCG AGGATGGAGC CTGGAGCGGC AAGCAGCCGC GCTGCGAATT GGCCACGGCG 
GAGGGTCAGG AGACTTCGGA GCTGGGCACG GGCGTTGGCA TTGGTGCCAC CGTGATCGTC 
GCCCTGCTGG TGATCTTTGG TCTGATCTTC CTATATCGCA ACAAGGCGAG ACCCGTCAAA 
AATACGGAGA ATGTGCAGGC GGCGGAGACG AAGGACGAAC GCAATGCCGC CGTAATGTCC 
TACTCGACGC TGGAGGCCAA CAATCGGATG CACATGGACA ACAATCCGTC GGCGACGTTC 
AACACGTTCC ATGGCGGAGC CGGCGGAAGG AGCAACGGCG GCGGTAATCC GGATGCAAAC 
AGCAATGGTG AAAGACTGAA CAACAATCGG TCGGAAAACA TTTACGATCA AATACCAAAC 
GAGCAATTCT ACGACGCTCC CTATGAGATG CGCACCAATG ATGAGGTTTA CGAACCGGAA 
CCGGTGGCCA GTAATGTCAT CACCATCAAT GGGATTTCCG TCAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001096961.1
CDS	1089..3356
Translation	MGKKFTLGEKIQYTCPKGHSLLGQTERECRLDGTWSGSSPTCKYVDCGSLPELKFGSIHMSEERTSFGVVATYSCHENYTLIGNENRTCAMDGWSGKQPECLVDWCPDPQPIAGGDVRFNDKRAGSTATYVCEPGYVLVGEAIISCGLGGEWSSKTPSCRFVDCGAPARPNRGIAILLNGTTTVNSVVKYECDEDHWLDGQSELYCTREGKWSGEAPVCELVTCETPSVPSGSFVIGYDYNVHSKIQYNCDPGHIMHGTPVLECLDSGEWSADAPYCEYIDCGTILPIPYGSHKYVTNTTYVGSEVVFSCSQSHKLSGVLKRTCLESAVWSDASPKCEEIRCPEPKLAAHSLLSVTGNDRMYGRTLIRTSESSQNTAQTYKIGALAKYRCERGYKMVGEALATCTDSGQWSGTIPECVYVECGAPEGINNGKVVLATNATYYGAAVLYECNVNFKLNGVSRRLCTEHGNWSHEAPECVEVVCDTPNINENLIVEAGPRAVGSVATFKCAKGRIMMGNDTRVCQKNGKWTGKSPTCRPVDCGRPLAIENGRVIVVNDSTLYGGSAEYHCIPNYNRIGQYLRKCTEDGAWSGKQPRCELATAEGQETSELGTGVGIGATVIVALLVIFGLIFLYRNKARPVKNTENVQAAETKDERNAAVMSYSTLEANNRMHMDNNPSATFNTFHGGAGGRSNGGGNPDANSNGERLNNNRSENIYDQIPNEQFYDAPYEMRTNDEVYEPEPVASNVITINGISVR

Target ORF information:

RefSeq Version	NM_001103491.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster furrowed (fw), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103491.1

ORF Insert Sequence:

ATGGGCAAAA AGTTCACTCT GGGCGAGAAG ATTCAGTACA CCTGTCCCAA GGGCCATTCG 
CTGCTCGGTC AGACGGAACG GGAGTGTAGA CTTGATGGCA CTTGGAGTGG CTCCTCGCCG 
ACCTGCAAAT ATGTGGACTG CGGCAGTCTG CCGGAGCTGA AGTTTGGATC CATCCACATG 
TCGGAGGAGC GGACTAGCTT CGGCGTGGTG GCCACATACA GTTGCCACGA GAACTACACG 
CTAATCGGGA ATGAGAATCG CACGTGCGCC ATGGACGGCT GGAGCGGCAA GCAGCCGGAA 
TGCCTGGTGG ACTGGTGTCC AGATCCGCAG CCCATAGCCG GTGGCGATGT GCGATTCAAT 
GACAAGCGTG CCGGTTCCAC GGCCACGTAT GTTTGTGAGC CGGGCTATGT GCTAGTTGGC 
GAGGCGATCA TCTCGTGCGG TTTGGGCGGC GAGTGGTCCA GCAAGACGCC CTCGTGCAGA 
TTCGTCGATT GTGGTGCACC GGCACGACCA AATCGTGGCA TTGCCATCCT GCTGAACGGC 
ACCACCACCG TCAATTCGGT GGTTAAATAC GAGTGCGACG AGGATCATTG GCTGGACGGG 
CAGTCGGAGC TCTATTGCAC CCGGGAAGGG AAGTGGTCGG GTGAGGCGCC CGTCTGTGAA 
CTGGTCACCT GCGAGACGCC ATCGGTGCCA TCCGGTTCGT TTGTCATTGG CTACGATTAC 
AATGTCCATT CGAAGATCCA GTACAACTGC GATCCCGGTC ACATTATGCA CGGCACTCCG 
GTGCTCGAGT GCCTGGACTC CGGGGAATGG AGTGCCGATG CGCCCTACTG CGAATATATC 
GACTGCGGCA CAATCCTGCC CATTCCCTAT GGCAGCCACA AGTACGTGAC GAATACCACC 
TATGTGGGCT CCGAAGTGGT CTTCAGCTGC TCCCAATCGC ACAAACTGAG CGGAGTCCTC 
AAGCGCACGT GCCTGGAATC GGCTGTCTGG AGCGATGCGT CGCCCAAGTG CGAGGAGATT 
CGCTGTCCGG AACCGAAACT GGCTGCGCAC AGTCTGCTCT CCGTCACCGG AAATGATCGC 
ATGTATGGAA GGACACTTAT CCGCACCTCC GAATCCTCGC AGAACACAGC TCAAACCTAT 
AAGATTGGAG CACTGGCCAA GTACCGATGC GAACGGGGCT ACAAGATGGT GGGCGAGGCA 
CTGGCCACCT GCACGGATAG TGGACAATGG AGCGGCACCA TTCCGGAATG TGTGTATGTG 
GAGTGCGGTG CTCCGGAGGG CATCAACAAT GGCAAGGTGG TCCTGGCCAC AAATGCCACG 
TACTACGGCG CCGCTGTCCT CTATGAGTGC AATGTGAACT TCAAGCTAAA CGGAGTATCC 
CGACGCCTGT GCACGGAGCA CGGCAACTGG AGCCACGAGG CACCGGAATG CGTGGAGGTG 
GTCTGCGATA CGCCCAACAT CAATGAGAAT CTGATCGTGG AGGCGGGTCC GCGTGCTGTG 
GGCTCCGTGG CCACCTTCAA GTGCGCCAAG GGCAGGATCA TGATGGGAAA CGATACGCGT 
GTGTGTCAGA AAAATGGCAA ATGGACTGGC AAGAGTCCCA CCTGCAGACC CGTCGACTGT 
GGTCGTCCTC TGGCCATCGA GAATGGTCGC GTGATTGTGG TCAACGATTC CACGTTGTAC 
GGCGGATCGG CGGAGTATCA CTGCATACCG AACTACAATC GGATTGGCCA GTATTTGCGC 
AAGTGCACCG AGGATGGAGC CTGGAGCGGC AAGCAGCCGC GCTGCGAATT GGCCACGGCG 
GAGGGTCAGG AGACTTCGGA GCTGGGCACG GGCGTTGGCA TTGGTGCCAC CGTGATCGTC 
GCCCTGCTGG TGATCTTTGG TCTGATCTTC CTATATCGCA ACAAGGCGAG ACCCGTCAAA 
AATACGGAGA ATGTGCAGGC GGCGGAGACG AAGGACGAAC GCAATGCCGC CGTAATGTCC 
TACTCGACGC TGGAGGCCAA CAATCGGATG CACATGGACA ACAATCCGTC GGCGACGTTC 
AACACGTTCC ATGGCGGAGC CGGCGGAAGG AGCAACGGCG GCGGTAATCC GGATGCAAAC 
AGCAATGGTG AAAGACTGAA CAACAATCGG TCGGAAAACA TTTACGATCA AATACCAAAC 
GAGCAATTCT ACGACGCTCC CTATGAGATG CGCACCAATG ATGAGGTTTA CGAACCGGAA 
CCGGTGGCCA GTAATGTCAT CACCATCAAT GGGATTTCCG TCAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fw ( NM_001298217.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285146.1 Drosophila melanogaster furrowed (fw), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa01363
Clone ID Related Accession (Same CDS sequence)	NM_001298217.1 , NM_078581.3
Accession Version	NM_001298217.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	furrowed, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTTGCT GCAACATACC GGTGGATGCA TCCGTGGCCA CATCCACATC CACATCCATA 
TCCACATATC CATTGCAACG GGCAACAAAA AGAGCCATGA CTCCTCCAAA TGCAATGGCA 
CTCATTATGC TACTGCTGCT TGCACTGTGC GCAGCTGGGA ATGGCCAAAT CTGCGGTCCG 
CCGGCGGTGC CGCTGAATGC CAAGGTGCGA ACCATCACCG TTGACTCCCA GATCGCGGAG 
GCGCACTACG AGTGCGACGC TGGCTACGAG CTCTTCGGGC CATCGGCGGT CAAGTGTGAC 
CGGCGGACGG GCTGGGATCG GGACCTGCCC TTCTGCGGGG TAAATGTGGC GTACCGCAAG 
CCGGTCAACC AGAGCTCCTA TACGCGCAGC GGTCCTGCCA GCTATGCGAA CGATGGCAAG 
CCGGGCAACA AGAATCCCGA TGGCCAGGAG TGCTCCGAGA CGCAGAAGGA GCCATCGCCG 
TGGTGGCGCG TCGACCTTCT CACTCCGCAG GCGGTGCACG TGGTGCGGAT AACGACGCGC 
GGCTGCTGCG GCCATCAGCC ACTGCAGGAT CTGGAGATCC GTGTGGGCAA CAGCAGTGCG 
GATCTGCAGC GAAATCCGTT GTGCGCCTGG TATCCGGGCA CCCTGGACGA GGGCGTGGTG 
AAGACCTTCA CGTGCGCGCG CCCATTGGTC GGCCAGTATG TGGCCATTCA GCTGGTGGGT 
GTGGAGGGCA GCCTCAGTTT GTGCGAGGTG GAGACCTTCA CCAACGACGA GTTCTCCGTG 
GATCGATGCC TGAGTCCGAA AATCGGTGTG GACACTGTGG TAACGACATT CTCGAAAACC 
TGCTACGACT TTCATATCAC AAAAGGAGAA AGCTTCGACA AGGCGCAAGC CATTTGCAAG 
CAAACTGGTG GCGACTTGGT ACACGACTTT CGTGGCGCCA CCAGTTCGTA CATCTTGTCC 
GAGCTGGAAC GCCGGAAGAG CGAACTGAAA CCGCAGCTTG TGTGGATTGG CGCCCAAAAG 
GAGCCGGGCA TTACATCGCG CACCTGGAAA TGGGTGAACG GCGATGTGGT TCAAAAGCCA 
ACATGGGGCA AGGACCAGCC GAACAACTAC AATGGCGAGC AGAATTGCGT GGTCCTCGAT 
GGAGGTCGCA ATTGGCTGTG GAATGATGTG GGCTGCAATC TGGACTATTT GCACTTCATC 
TGCCAGCACT CGCCATTGTC TTGCGGCTCG CCGGATGCCC AGCAAAACAC CACTGTCATG 
GGCAAAAAGT TCACTCTGGG CGAGAAGATT CAGTACACCT GTCCCAAGGG CCATTCGCTG 
CTCGGTCAGA CGGAACGGGA GTGTAGACTT GATGGCACTT GGAGTGGCTC CTCGCCGACC 
TGCAAATATG TGGACTGCGG CAGTCTGCCG GAGCTGAAGT TTGGATCCAT CCACATGTCG 
GAGGAGCGGA CTAGCTTCGG CGTGGTGGCC ACATACAGTT GCCACGAGAA CTACACGCTA 
ATCGGGAATG AGAATCGCAC GTGCGCCATG GACGGCTGGA GCGGCAAGCA GCCGGAATGC 
CTGGTGGACT GGTGTCCAGA TCCGCAGCCC ATAGCCGGTG GCGATGTGCG ATTCAATGAC 
AAGCGTGCCG GTTCCACGGC CACGTATGTT TGTGAGCCGG GCTATGTGCT AGTTGGCGAG 
GCGATCATCT CGTGCGGTTT GGGCGGCGAG TGGTCCAGCA AGACGCCCTC GTGCAGATTC 
GTCGATTGTG GTGCACCGGC ACGACCAAAT CGTGGCATTG CCATCCTGCT GAACGGCACC 
ACCACCGTCA ATTCGGTGGT TAAATACGAG TGCGACGAGG ATCATTGGCT GGACGGGCAG 
TCGGAGCTCT ATTGCACCCG GGAAGGGAAG TGGTCGGGTG AGGCGCCCGT CTGTGAACTG 
GTCACCTGCG AGACGCCATC GGTGCCATCC GGTTCGTTTG TCATTGGCTA CGATTACAAT 
GTCCATTCGA AGATCCAGTA CAACTGCGAT CCCGGTCACA TTATGCACGG CACTCCGGTG 
CTCGAGTGCC TGGACTCCGG GGAATGGAGT GCCGATGCGC CCTACTGCGA ATATATCGAC 
TGCGGCACAA TCCTGCCCAT TCCCTATGGC AGCCACAAGT ACGTGACGAA TACCACCTAT 
GTGGGCTCCG AAGTGGTCTT CAGCTGCTCC CAATCGCACA AACTGAGCGG AGTCCTCAAG 
CGCACGTGCC TGGAATCGGC TGTCTGGAGC GATGCGTCGC CCAAGTGCGA GGAGATTCGC 
TGTCCGGAAC CGAAACTGGC TGCGCACAGT CTGCTCTCCG TCACCGGAAA TGATCGCATG 
TATGGAAGGA CACTTATCCG CACCTCCGAA TCCTCGCAGA ACACAGCTCA AACCTATAAG 
ATTGGAGCAC TGGCCAAGTA CCGATGCGAA CGGGGCTACA AGATGGTGGG CGAGGCACTG 
GCCACCTGCA CGGATAGTGG ACAATGGAGC GGCACCATTC CGGAATGTGT GTATGTGGAG 
TGCGGTGCTC CGGAGGGCAT CAACAATGGC AAGGTGGTCC TGGCCACAAA TGCCACGTAC 
TACGGCGCCG CTGTCCTCTA TGAGTGCAAT GTGAACTTCA AGCTAAACGG AGTATCCCGA 
CGCCTGTGCA CGGAGCACGG CAACTGGAGC CACGAGGCAC CGGAATGCGT GGAGGTGGTC 
TGCGATACGC CCAACATCAA TGAGAATCTG ATCGTGGAGG CGGGTCCGCG TGCTGTGGGC 
TCCGTGGCCA CCTTCAAGTG CGCCAAGGGC AGGATCATGA TGGGAAACGA TACGCGTGTG 
TGTCAGAAAA ATGGCAAATG GACTGGCAAG AGTCCCACCT GCAGACCCGT CGACTGTGGT 
CGTCCTCTGG CCATCGAGAA TGGTCGCGTG ATTGTGGTCA ACGATTCCAC GTTGTACGGC 
GGATCGGCGG AGTATCACTG CATACCGAAC TACAATCGGA TTGGCCAGTA TTTGCGCAAG 
TGCACCGAGG ATGGAGCCTG GAGCGGCAAG CAGCCGCGCT GCGAATTGGC CACGGCGGAG 
GGTCAGGAGA CTTCGGAGCT GGGCACGGGC GTTGGCATTG GTGCCACCGT GATCGTCGCC 
CTGCTGGTGA TCTTTGGTCT GATCTTCCTA TATCGCAACA AGGCGAGACC CGTCAAAAAT 
ACGGAGAATG TGCAGGCGGC GGAGACGAAG GACGAACGCA ATGCCGCCGT AATGTCCTAC 
TCGACGCTGG AGGCCAACAA TCGGATGCAC ATGGACAACA ATCCGTCGGC GACGTTCAAC 
ACGTTCCATG GCGGAGCCGG CGGAAGGAGC AACGGCGGCG GTAATCCGGA TGCAAACAGC 
AATGGTGAAA GACTGAACAA CAATCGGTCG GAAAACATTT ACGATCAAAT ACCAAACGAG 
CAATTCTACG ACGCTCCCTA TGAGATGCGC ACCAATGATG AGGTTTACGA ACCGGAACCG 
GTGGCCAGTA ATGTCATCAC CATCAATGGG ATTTCCGTCA GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285146.1
CDS	212..3736
Translation	MFCCNIPVDASVATSTSTSISTYPLQRATKRAMTPPNAMALIMLLLLALCAAGNGQICGPPAVPLNAKVRTITVDSQIAEAHYECDAGYELFGPSAVKCDRRTGWDRDLPFCGVNVAYRKPVNQSSYTRSGPASYANDGKPGNKNPDGQECSETQKEPSPWWRVDLLTPQAVHVVRITTRGCCGHQPLQDLEIRVGNSSADLQRNPLCAWYPGTLDEGVVKTFTCARPLVGQYVAIQLVGVEGSLSLCEVETFTNDEFSVDRCLSPKIGVDTVVTTFSKTCYDFHITKGESFDKAQAICKQTGGDLVHDFRGATSSYILSELERRKSELKPQLVWIGAQKEPGITSRTWKWVNGDVVQKPTWGKDQPNNYNGEQNCVVLDGGRNWLWNDVGCNLDYLHFICQHSPLSCGSPDAQQNTTVMGKKFTLGEKIQYTCPKGHSLLGQTERECRLDGTWSGSSPTCKYVDCGSLPELKFGSIHMSEERTSFGVVATYSCHENYTLIGNENRTCAMDGWSGKQPECLVDWCPDPQPIAGGDVRFNDKRAGSTATYVCEPGYVLVGEAIISCGLGGEWSSKTPSCRFVDCGAPARPNRGIAILLNGTTTVNSVVKYECDEDHWLDGQSELYCTREGKWSGEAPVCELVTCETPSVPSGSFVIGYDYNVHSKIQYNCDPGHIMHGTPVLECLDSGEWSADAPYCEYIDCGTILPIPYGSHKYVTNTTYVGSEVVFSCSQSHKLSGVLKRTCLESAVWSDASPKCEEIRCPEPKLAAHSLLSVTGNDRMYGRTLIRTSESSQNTAQTYKIGALAKYRCERGYKMVGEALATCTDSGQWSGTIPECVYVECGAPEGINNGKVVLATNATYYGAAVLYECNVNFKLNGVSRRLCTEHGNWSHEAPECVEVVCDTPNINENLIVEAGPRAVGSVATFKCAKGRIMMGNDTRVCQKNGKWTGKSPTCRPVDCGRPLAIENGRVIVVNDSTLYGGSAEYHCIPNYNRIGQYLRKCTEDGAWSGKQPRCELATAEGQETSELGTGVGIGATVIVALLVIFGLIFLYRNKARPVKNTENVQAAETKDERNAAVMSYSTLEANNRMHMDNNPSATFNTFHGGAGGRSNGGGNPDANSNGERLNNNRSENIYDQIPNEQFYDAPYEMRTNDEVYEPEPVASNVITINGISVR

Target ORF information:

RefSeq Version	NM_001298217.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster furrowed (fw), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298217.1

ORF Insert Sequence:

ATGTTTTGCT GCAACATACC GGTGGATGCA TCCGTGGCCA CATCCACATC CACATCCATA 
TCCACATATC CATTGCAACG GGCAACAAAA AGAGCCATGA CTCCTCCAAA TGCAATGGCA 
CTCATTATGC TACTGCTGCT TGCACTGTGC GCAGCTGGGA ATGGCCAAAT CTGCGGTCCG 
CCGGCGGTGC CGCTGAATGC CAAGGTGCGA ACCATCACCG TTGACTCCCA GATCGCGGAG 
GCGCACTACG AGTGCGACGC TGGCTACGAG CTCTTCGGGC CATCGGCGGT CAAGTGTGAC 
CGGCGGACGG GCTGGGATCG GGACCTGCCC TTCTGCGGGG TAAATGTGGC GTACCGCAAG 
CCGGTCAACC AGAGCTCCTA TACGCGCAGC GGTCCTGCCA GCTATGCGAA CGATGGCAAG 
CCGGGCAACA AGAATCCCGA TGGCCAGGAG TGCTCCGAGA CGCAGAAGGA GCCATCGCCG 
TGGTGGCGCG TCGACCTTCT CACTCCGCAG GCGGTGCACG TGGTGCGGAT AACGACGCGC 
GGCTGCTGCG GCCATCAGCC ACTGCAGGAT CTGGAGATCC GTGTGGGCAA CAGCAGTGCG 
GATCTGCAGC GAAATCCGTT GTGCGCCTGG TATCCGGGCA CCCTGGACGA GGGCGTGGTG 
AAGACCTTCA CGTGCGCGCG CCCATTGGTC GGCCAGTATG TGGCCATTCA GCTGGTGGGT 
GTGGAGGGCA GCCTCAGTTT GTGCGAGGTG GAGACCTTCA CCAACGACGA GTTCTCCGTG 
GATCGATGCC TGAGTCCGAA AATCGGTGTG GACACTGTGG TAACGACATT CTCGAAAACC 
TGCTACGACT TTCATATCAC AAAAGGAGAA AGCTTCGACA AGGCGCAAGC CATTTGCAAG 
CAAACTGGTG GCGACTTGGT ACACGACTTT CGTGGCGCCA CCAGTTCGTA CATCTTGTCC 
GAGCTGGAAC GCCGGAAGAG CGAACTGAAA CCGCAGCTTG TGTGGATTGG CGCCCAAAAG 
GAGCCGGGCA TTACATCGCG CACCTGGAAA TGGGTGAACG GCGATGTGGT TCAAAAGCCA 
ACATGGGGCA AGGACCAGCC GAACAACTAC AATGGCGAGC AGAATTGCGT GGTCCTCGAT 
GGAGGTCGCA ATTGGCTGTG GAATGATGTG GGCTGCAATC TGGACTATTT GCACTTCATC 
TGCCAGCACT CGCCATTGTC TTGCGGCTCG CCGGATGCCC AGCAAAACAC CACTGTCATG 
GGCAAAAAGT TCACTCTGGG CGAGAAGATT CAGTACACCT GTCCCAAGGG CCATTCGCTG 
CTCGGTCAGA CGGAACGGGA GTGTAGACTT GATGGCACTT GGAGTGGCTC CTCGCCGACC 
TGCAAATATG TGGACTGCGG CAGTCTGCCG GAGCTGAAGT TTGGATCCAT CCACATGTCG 
GAGGAGCGGA CTAGCTTCGG CGTGGTGGCC ACATACAGTT GCCACGAGAA CTACACGCTA 
ATCGGGAATG AGAATCGCAC GTGCGCCATG GACGGCTGGA GCGGCAAGCA GCCGGAATGC 
CTGGTGGACT GGTGTCCAGA TCCGCAGCCC ATAGCCGGTG GCGATGTGCG ATTCAATGAC 
AAGCGTGCCG GTTCCACGGC CACGTATGTT TGTGAGCCGG GCTATGTGCT AGTTGGCGAG 
GCGATCATCT CGTGCGGTTT GGGCGGCGAG TGGTCCAGCA AGACGCCCTC GTGCAGATTC 
GTCGATTGTG GTGCACCGGC ACGACCAAAT CGTGGCATTG CCATCCTGCT GAACGGCACC 
ACCACCGTCA ATTCGGTGGT TAAATACGAG TGCGACGAGG ATCATTGGCT GGACGGGCAG 
TCGGAGCTCT ATTGCACCCG GGAAGGGAAG TGGTCGGGTG AGGCGCCCGT CTGTGAACTG 
GTCACCTGCG AGACGCCATC GGTGCCATCC GGTTCGTTTG TCATTGGCTA CGATTACAAT 
GTCCATTCGA AGATCCAGTA CAACTGCGAT CCCGGTCACA TTATGCACGG CACTCCGGTG 
CTCGAGTGCC TGGACTCCGG GGAATGGAGT GCCGATGCGC CCTACTGCGA ATATATCGAC 
TGCGGCACAA TCCTGCCCAT TCCCTATGGC AGCCACAAGT ACGTGACGAA TACCACCTAT 
GTGGGCTCCG AAGTGGTCTT CAGCTGCTCC CAATCGCACA AACTGAGCGG AGTCCTCAAG 
CGCACGTGCC TGGAATCGGC TGTCTGGAGC GATGCGTCGC CCAAGTGCGA GGAGATTCGC 
TGTCCGGAAC CGAAACTGGC TGCGCACAGT CTGCTCTCCG TCACCGGAAA TGATCGCATG 
TATGGAAGGA CACTTATCCG CACCTCCGAA TCCTCGCAGA ACACAGCTCA AACCTATAAG 
ATTGGAGCAC TGGCCAAGTA CCGATGCGAA CGGGGCTACA AGATGGTGGG CGAGGCACTG 
GCCACCTGCA CGGATAGTGG ACAATGGAGC GGCACCATTC CGGAATGTGT GTATGTGGAG 
TGCGGTGCTC CGGAGGGCAT CAACAATGGC AAGGTGGTCC TGGCCACAAA TGCCACGTAC 
TACGGCGCCG CTGTCCTCTA TGAGTGCAAT GTGAACTTCA AGCTAAACGG AGTATCCCGA 
CGCCTGTGCA CGGAGCACGG CAACTGGAGC CACGAGGCAC CGGAATGCGT GGAGGTGGTC 
TGCGATACGC CCAACATCAA TGAGAATCTG ATCGTGGAGG CGGGTCCGCG TGCTGTGGGC 
TCCGTGGCCA CCTTCAAGTG CGCCAAGGGC AGGATCATGA TGGGAAACGA TACGCGTGTG 
TGTCAGAAAA ATGGCAAATG GACTGGCAAG AGTCCCACCT GCAGACCCGT CGACTGTGGT 
CGTCCTCTGG CCATCGAGAA TGGTCGCGTG ATTGTGGTCA ACGATTCCAC GTTGTACGGC 
GGATCGGCGG AGTATCACTG CATACCGAAC TACAATCGGA TTGGCCAGTA TTTGCGCAAG 
TGCACCGAGG ATGGAGCCTG GAGCGGCAAG CAGCCGCGCT GCGAATTGGC CACGGCGGAG 
GGTCAGGAGA CTTCGGAGCT GGGCACGGGC GTTGGCATTG GTGCCACCGT GATCGTCGCC 
CTGCTGGTGA TCTTTGGTCT GATCTTCCTA TATCGCAACA AGGCGAGACC CGTCAAAAAT 
ACGGAGAATG TGCAGGCGGC GGAGACGAAG GACGAACGCA ATGCCGCCGT AATGTCCTAC 
TCGACGCTGG AGGCCAACAA TCGGATGCAC ATGGACAACA ATCCGTCGGC GACGTTCAAC 
ACGTTCCATG GCGGAGCCGG CGGAAGGAGC AACGGCGGCG GTAATCCGGA TGCAAACAGC 
AATGGTGAAA GACTGAACAA CAATCGGTCG GAAAACATTT ACGATCAAAT ACCAAACGAG 
CAATTCTACG ACGCTCCCTA TGAGATGCGC ACCAATGATG AGGTTTACGA ACCGGAACCG 
GTGGCCAGTA ATGTCATCAC CATCAATGGG ATTTCCGTCA GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fw ( NM_078581.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_511136.2 Drosophila melanogaster furrowed (fw), transcript variant A, mRNA.

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CloneID	OFa01363
Clone ID Related Accession (Same CDS sequence)	NM_001298217.1 , NM_078581.3
Accession Version	NM_078581.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	furrowed, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_078581.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTTGCT GCAACATACC GGTGGATGCA TCCGTGGCCA CATCCACATC CACATCCATA 
TCCACATATC CATTGCAACG GGCAACAAAA AGAGCCATGA CTCCTCCAAA TGCAATGGCA 
CTCATTATGC TACTGCTGCT TGCACTGTGC GCAGCTGGGA ATGGCCAAAT CTGCGGTCCG 
CCGGCGGTGC CGCTGAATGC CAAGGTGCGA ACCATCACCG TTGACTCCCA GATCGCGGAG 
GCGCACTACG AGTGCGACGC TGGCTACGAG CTCTTCGGGC CATCGGCGGT CAAGTGTGAC 
CGGCGGACGG GCTGGGATCG GGACCTGCCC TTCTGCGGGG TAAATGTGGC GTACCGCAAG 
CCGGTCAACC AGAGCTCCTA TACGCGCAGC GGTCCTGCCA GCTATGCGAA CGATGGCAAG 
CCGGGCAACA AGAATCCCGA TGGCCAGGAG TGCTCCGAGA CGCAGAAGGA GCCATCGCCG 
TGGTGGCGCG TCGACCTTCT CACTCCGCAG GCGGTGCACG TGGTGCGGAT AACGACGCGC 
GGCTGCTGCG GCCATCAGCC ACTGCAGGAT CTGGAGATCC GTGTGGGCAA CAGCAGTGCG 
GATCTGCAGC GAAATCCGTT GTGCGCCTGG TATCCGGGCA CCCTGGACGA GGGCGTGGTG 
AAGACCTTCA CGTGCGCGCG CCCATTGGTC GGCCAGTATG TGGCCATTCA GCTGGTGGGT 
GTGGAGGGCA GCCTCAGTTT GTGCGAGGTG GAGACCTTCA CCAACGACGA GTTCTCCGTG 
GATCGATGCC TGAGTCCGAA AATCGGTGTG GACACTGTGG TAACGACATT CTCGAAAACC 
TGCTACGACT TTCATATCAC AAAAGGAGAA AGCTTCGACA AGGCGCAAGC CATTTGCAAG 
CAAACTGGTG GCGACTTGGT ACACGACTTT CGTGGCGCCA CCAGTTCGTA CATCTTGTCC 
GAGCTGGAAC GCCGGAAGAG CGAACTGAAA CCGCAGCTTG TGTGGATTGG CGCCCAAAAG 
GAGCCGGGCA TTACATCGCG CACCTGGAAA TGGGTGAACG GCGATGTGGT TCAAAAGCCA 
ACATGGGGCA AGGACCAGCC GAACAACTAC AATGGCGAGC AGAATTGCGT GGTCCTCGAT 
GGAGGTCGCA ATTGGCTGTG GAATGATGTG GGCTGCAATC TGGACTATTT GCACTTCATC 
TGCCAGCACT CGCCATTGTC TTGCGGCTCG CCGGATGCCC AGCAAAACAC CACTGTCATG 
GGCAAAAAGT TCACTCTGGG CGAGAAGATT CAGTACACCT GTCCCAAGGG CCATTCGCTG 
CTCGGTCAGA CGGAACGGGA GTGTAGACTT GATGGCACTT GGAGTGGCTC CTCGCCGACC 
TGCAAATATG TGGACTGCGG CAGTCTGCCG GAGCTGAAGT TTGGATCCAT CCACATGTCG 
GAGGAGCGGA CTAGCTTCGG CGTGGTGGCC ACATACAGTT GCCACGAGAA CTACACGCTA 
ATCGGGAATG AGAATCGCAC GTGCGCCATG GACGGCTGGA GCGGCAAGCA GCCGGAATGC 
CTGGTGGACT GGTGTCCAGA TCCGCAGCCC ATAGCCGGTG GCGATGTGCG ATTCAATGAC 
AAGCGTGCCG GTTCCACGGC CACGTATGTT TGTGAGCCGG GCTATGTGCT AGTTGGCGAG 
GCGATCATCT CGTGCGGTTT GGGCGGCGAG TGGTCCAGCA AGACGCCCTC GTGCAGATTC 
GTCGATTGTG GTGCACCGGC ACGACCAAAT CGTGGCATTG CCATCCTGCT GAACGGCACC 
ACCACCGTCA ATTCGGTGGT TAAATACGAG TGCGACGAGG ATCATTGGCT GGACGGGCAG 
TCGGAGCTCT ATTGCACCCG GGAAGGGAAG TGGTCGGGTG AGGCGCCCGT CTGTGAACTG 
GTCACCTGCG AGACGCCATC GGTGCCATCC GGTTCGTTTG TCATTGGCTA CGATTACAAT 
GTCCATTCGA AGATCCAGTA CAACTGCGAT CCCGGTCACA TTATGCACGG CACTCCGGTG 
CTCGAGTGCC TGGACTCCGG GGAATGGAGT GCCGATGCGC CCTACTGCGA ATATATCGAC 
TGCGGCACAA TCCTGCCCAT TCCCTATGGC AGCCACAAGT ACGTGACGAA TACCACCTAT 
GTGGGCTCCG AAGTGGTCTT CAGCTGCTCC CAATCGCACA AACTGAGCGG AGTCCTCAAG 
CGCACGTGCC TGGAATCGGC TGTCTGGAGC GATGCGTCGC CCAAGTGCGA GGAGATTCGC 
TGTCCGGAAC CGAAACTGGC TGCGCACAGT CTGCTCTCCG TCACCGGAAA TGATCGCATG 
TATGGAAGGA CACTTATCCG CACCTCCGAA TCCTCGCAGA ACACAGCTCA AACCTATAAG 
ATTGGAGCAC TGGCCAAGTA CCGATGCGAA CGGGGCTACA AGATGGTGGG CGAGGCACTG 
GCCACCTGCA CGGATAGTGG ACAATGGAGC GGCACCATTC CGGAATGTGT GTATGTGGAG 
TGCGGTGCTC CGGAGGGCAT CAACAATGGC AAGGTGGTCC TGGCCACAAA TGCCACGTAC 
TACGGCGCCG CTGTCCTCTA TGAGTGCAAT GTGAACTTCA AGCTAAACGG AGTATCCCGA 
CGCCTGTGCA CGGAGCACGG CAACTGGAGC CACGAGGCAC CGGAATGCGT GGAGGTGGTC 
TGCGATACGC CCAACATCAA TGAGAATCTG ATCGTGGAGG CGGGTCCGCG TGCTGTGGGC 
TCCGTGGCCA CCTTCAAGTG CGCCAAGGGC AGGATCATGA TGGGAAACGA TACGCGTGTG 
TGTCAGAAAA ATGGCAAATG GACTGGCAAG AGTCCCACCT GCAGACCCGT CGACTGTGGT 
CGTCCTCTGG CCATCGAGAA TGGTCGCGTG ATTGTGGTCA ACGATTCCAC GTTGTACGGC 
GGATCGGCGG AGTATCACTG CATACCGAAC TACAATCGGA TTGGCCAGTA TTTGCGCAAG 
TGCACCGAGG ATGGAGCCTG GAGCGGCAAG CAGCCGCGCT GCGAATTGGC CACGGCGGAG 
GGTCAGGAGA CTTCGGAGCT GGGCACGGGC GTTGGCATTG GTGCCACCGT GATCGTCGCC 
CTGCTGGTGA TCTTTGGTCT GATCTTCCTA TATCGCAACA AGGCGAGACC CGTCAAAAAT 
ACGGAGAATG TGCAGGCGGC GGAGACGAAG GACGAACGCA ATGCCGCCGT AATGTCCTAC 
TCGACGCTGG AGGCCAACAA TCGGATGCAC ATGGACAACA ATCCGTCGGC GACGTTCAAC 
ACGTTCCATG GCGGAGCCGG CGGAAGGAGC AACGGCGGCG GTAATCCGGA TGCAAACAGC 
AATGGTGAAA GACTGAACAA CAATCGGTCG GAAAACATTT ACGATCAAAT ACCAAACGAG 
CAATTCTACG ACGCTCCCTA TGAGATGCGC ACCAATGATG AGGTTTACGA ACCGGAACCG 
GTGGCCAGTA ATGTCATCAC CATCAATGGG ATTTCCGTCA GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_511136.2
CDS	592..4116
Translation	MFCCNIPVDASVATSTSTSISTYPLQRATKRAMTPPNAMALIMLLLLALCAAGNGQICGPPAVPLNAKVRTITVDSQIAEAHYECDAGYELFGPSAVKCDRRTGWDRDLPFCGVNVAYRKPVNQSSYTRSGPASYANDGKPGNKNPDGQECSETQKEPSPWWRVDLLTPQAVHVVRITTRGCCGHQPLQDLEIRVGNSSADLQRNPLCAWYPGTLDEGVVKTFTCARPLVGQYVAIQLVGVEGSLSLCEVETFTNDEFSVDRCLSPKIGVDTVVTTFSKTCYDFHITKGESFDKAQAICKQTGGDLVHDFRGATSSYILSELERRKSELKPQLVWIGAQKEPGITSRTWKWVNGDVVQKPTWGKDQPNNYNGEQNCVVLDGGRNWLWNDVGCNLDYLHFICQHSPLSCGSPDAQQNTTVMGKKFTLGEKIQYTCPKGHSLLGQTERECRLDGTWSGSSPTCKYVDCGSLPELKFGSIHMSEERTSFGVVATYSCHENYTLIGNENRTCAMDGWSGKQPECLVDWCPDPQPIAGGDVRFNDKRAGSTATYVCEPGYVLVGEAIISCGLGGEWSSKTPSCRFVDCGAPARPNRGIAILLNGTTTVNSVVKYECDEDHWLDGQSELYCTREGKWSGEAPVCELVTCETPSVPSGSFVIGYDYNVHSKIQYNCDPGHIMHGTPVLECLDSGEWSADAPYCEYIDCGTILPIPYGSHKYVTNTTYVGSEVVFSCSQSHKLSGVLKRTCLESAVWSDASPKCEEIRCPEPKLAAHSLLSVTGNDRMYGRTLIRTSESSQNTAQTYKIGALAKYRCERGYKMVGEALATCTDSGQWSGTIPECVYVECGAPEGINNGKVVLATNATYYGAAVLYECNVNFKLNGVSRRLCTEHGNWSHEAPECVEVVCDTPNINENLIVEAGPRAVGSVATFKCAKGRIMMGNDTRVCQKNGKWTGKSPTCRPVDCGRPLAIENGRVIVVNDSTLYGGSAEYHCIPNYNRIGQYLRKCTEDGAWSGKQPRCELATAEGQETSELGTGVGIGATVIVALLVIFGLIFLYRNKARPVKNTENVQAAETKDERNAAVMSYSTLEANNRMHMDNNPSATFNTFHGGAGGRSNGGGNPDANSNGERLNNNRSENIYDQIPNEQFYDAPYEMRTNDEVYEPEPVASNVITINGISVR

Target ORF information:

RefSeq Version	NM_078581.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster furrowed (fw), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078581.3

ORF Insert Sequence:

ATGTTTTGCT GCAACATACC GGTGGATGCA TCCGTGGCCA CATCCACATC CACATCCATA 
TCCACATATC CATTGCAACG GGCAACAAAA AGAGCCATGA CTCCTCCAAA TGCAATGGCA 
CTCATTATGC TACTGCTGCT TGCACTGTGC GCAGCTGGGA ATGGCCAAAT CTGCGGTCCG 
CCGGCGGTGC CGCTGAATGC CAAGGTGCGA ACCATCACCG TTGACTCCCA GATCGCGGAG 
GCGCACTACG AGTGCGACGC TGGCTACGAG CTCTTCGGGC CATCGGCGGT CAAGTGTGAC 
CGGCGGACGG GCTGGGATCG GGACCTGCCC TTCTGCGGGG TAAATGTGGC GTACCGCAAG 
CCGGTCAACC AGAGCTCCTA TACGCGCAGC GGTCCTGCCA GCTATGCGAA CGATGGCAAG 
CCGGGCAACA AGAATCCCGA TGGCCAGGAG TGCTCCGAGA CGCAGAAGGA GCCATCGCCG 
TGGTGGCGCG TCGACCTTCT CACTCCGCAG GCGGTGCACG TGGTGCGGAT AACGACGCGC 
GGCTGCTGCG GCCATCAGCC ACTGCAGGAT CTGGAGATCC GTGTGGGCAA CAGCAGTGCG 
GATCTGCAGC GAAATCCGTT GTGCGCCTGG TATCCGGGCA CCCTGGACGA GGGCGTGGTG 
AAGACCTTCA CGTGCGCGCG CCCATTGGTC GGCCAGTATG TGGCCATTCA GCTGGTGGGT 
GTGGAGGGCA GCCTCAGTTT GTGCGAGGTG GAGACCTTCA CCAACGACGA GTTCTCCGTG 
GATCGATGCC TGAGTCCGAA AATCGGTGTG GACACTGTGG TAACGACATT CTCGAAAACC 
TGCTACGACT TTCATATCAC AAAAGGAGAA AGCTTCGACA AGGCGCAAGC CATTTGCAAG 
CAAACTGGTG GCGACTTGGT ACACGACTTT CGTGGCGCCA CCAGTTCGTA CATCTTGTCC 
GAGCTGGAAC GCCGGAAGAG CGAACTGAAA CCGCAGCTTG TGTGGATTGG CGCCCAAAAG 
GAGCCGGGCA TTACATCGCG CACCTGGAAA TGGGTGAACG GCGATGTGGT TCAAAAGCCA 
ACATGGGGCA AGGACCAGCC GAACAACTAC AATGGCGAGC AGAATTGCGT GGTCCTCGAT 
GGAGGTCGCA ATTGGCTGTG GAATGATGTG GGCTGCAATC TGGACTATTT GCACTTCATC 
TGCCAGCACT CGCCATTGTC TTGCGGCTCG CCGGATGCCC AGCAAAACAC CACTGTCATG 
GGCAAAAAGT TCACTCTGGG CGAGAAGATT CAGTACACCT GTCCCAAGGG CCATTCGCTG 
CTCGGTCAGA CGGAACGGGA GTGTAGACTT GATGGCACTT GGAGTGGCTC CTCGCCGACC 
TGCAAATATG TGGACTGCGG CAGTCTGCCG GAGCTGAAGT TTGGATCCAT CCACATGTCG 
GAGGAGCGGA CTAGCTTCGG CGTGGTGGCC ACATACAGTT GCCACGAGAA CTACACGCTA 
ATCGGGAATG AGAATCGCAC GTGCGCCATG GACGGCTGGA GCGGCAAGCA GCCGGAATGC 
CTGGTGGACT GGTGTCCAGA TCCGCAGCCC ATAGCCGGTG GCGATGTGCG ATTCAATGAC 
AAGCGTGCCG GTTCCACGGC CACGTATGTT TGTGAGCCGG GCTATGTGCT AGTTGGCGAG 
GCGATCATCT CGTGCGGTTT GGGCGGCGAG TGGTCCAGCA AGACGCCCTC GTGCAGATTC 
GTCGATTGTG GTGCACCGGC ACGACCAAAT CGTGGCATTG CCATCCTGCT GAACGGCACC 
ACCACCGTCA ATTCGGTGGT TAAATACGAG TGCGACGAGG ATCATTGGCT GGACGGGCAG 
TCGGAGCTCT ATTGCACCCG GGAAGGGAAG TGGTCGGGTG AGGCGCCCGT CTGTGAACTG 
GTCACCTGCG AGACGCCATC GGTGCCATCC GGTTCGTTTG TCATTGGCTA CGATTACAAT 
GTCCATTCGA AGATCCAGTA CAACTGCGAT CCCGGTCACA TTATGCACGG CACTCCGGTG 
CTCGAGTGCC TGGACTCCGG GGAATGGAGT GCCGATGCGC CCTACTGCGA ATATATCGAC 
TGCGGCACAA TCCTGCCCAT TCCCTATGGC AGCCACAAGT ACGTGACGAA TACCACCTAT 
GTGGGCTCCG AAGTGGTCTT CAGCTGCTCC CAATCGCACA AACTGAGCGG AGTCCTCAAG 
CGCACGTGCC TGGAATCGGC TGTCTGGAGC GATGCGTCGC CCAAGTGCGA GGAGATTCGC 
TGTCCGGAAC CGAAACTGGC TGCGCACAGT CTGCTCTCCG TCACCGGAAA TGATCGCATG 
TATGGAAGGA CACTTATCCG CACCTCCGAA TCCTCGCAGA ACACAGCTCA AACCTATAAG 
ATTGGAGCAC TGGCCAAGTA CCGATGCGAA CGGGGCTACA AGATGGTGGG CGAGGCACTG 
GCCACCTGCA CGGATAGTGG ACAATGGAGC GGCACCATTC CGGAATGTGT GTATGTGGAG 
TGCGGTGCTC CGGAGGGCAT CAACAATGGC AAGGTGGTCC TGGCCACAAA TGCCACGTAC 
TACGGCGCCG CTGTCCTCTA TGAGTGCAAT GTGAACTTCA AGCTAAACGG AGTATCCCGA 
CGCCTGTGCA CGGAGCACGG CAACTGGAGC CACGAGGCAC CGGAATGCGT GGAGGTGGTC 
TGCGATACGC CCAACATCAA TGAGAATCTG ATCGTGGAGG CGGGTCCGCG TGCTGTGGGC 
TCCGTGGCCA CCTTCAAGTG CGCCAAGGGC AGGATCATGA TGGGAAACGA TACGCGTGTG 
TGTCAGAAAA ATGGCAAATG GACTGGCAAG AGTCCCACCT GCAGACCCGT CGACTGTGGT 
CGTCCTCTGG CCATCGAGAA TGGTCGCGTG ATTGTGGTCA ACGATTCCAC GTTGTACGGC 
GGATCGGCGG AGTATCACTG CATACCGAAC TACAATCGGA TTGGCCAGTA TTTGCGCAAG 
TGCACCGAGG ATGGAGCCTG GAGCGGCAAG CAGCCGCGCT GCGAATTGGC CACGGCGGAG 
GGTCAGGAGA CTTCGGAGCT GGGCACGGGC GTTGGCATTG GTGCCACCGT GATCGTCGCC 
CTGCTGGTGA TCTTTGGTCT GATCTTCCTA TATCGCAACA AGGCGAGACC CGTCAAAAAT 
ACGGAGAATG TGCAGGCGGC GGAGACGAAG GACGAACGCA ATGCCGCCGT AATGTCCTAC 
TCGACGCTGG AGGCCAACAA TCGGATGCAC ATGGACAACA ATCCGTCGGC GACGTTCAAC 
ACGTTCCATG GCGGAGCCGG CGGAAGGAGC AACGGCGGCG GTAATCCGGA TGCAAACAGC 
AATGGTGAAA GACTGAACAA CAATCGGTCG GAAAACATTT ACGATCAAAT ACCAAACGAG 
CAATTCTACG ACGCTCCCTA TGAGATGCGC ACCAATGATG AGGTTTACGA ACCGGAACCG 
GTGGCCAGTA ATGTCATCAC CATCAATGGG ATTTCCGTCA GATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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fw cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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