CG32373 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG32373
Entrez Gene ID	318000
Full Name	CG32373 gene product from transcript CG32373-RB
Synonyms	BcDNA:RE64043,CG3237,Dmel\CG32373
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 66A8-66A12

mRNA and Protein(s)

mRNA	Protein	Name
NM_168229.3	NP_729293.1	uncharacterized protein, isoform A
NM_206297.3	NP_996019.1	uncharacterized protein, isoform B
NM_001274605.1	NP_001261534.1	uncharacterized protein, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG32373 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG32373 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa19541	NM_168229.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG32373), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OFa19541	NM_206297.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG32373), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OFa19541	NM_001274605.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant C (CG32373), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG32373 ( NM_168229.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_729293.1 Drosophila melanogaster uncharacterized protein, transcript variant A (CG32373), mRNA.

Price & Availability↑

CloneID	OFa19541
Clone ID Related Accession (Same CDS sequence)	NM_168229.3 , NM_206297.3 , NM_001274605.1
Accession Version	NM_168229.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1461bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_168229.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGCAAA ACAGAATGTC GACATCAGTG ATTGTTTTGT ACGCGCTTAT GTGTATCGCA 
TTTTCCACAG CTTCGTTAAA TGATATTCCC GAAATCACCC CCGCAATAGA TAACAATTTA 
TCTTTTCAAT ACGACGATAC CTGCTGGACT CCTCCGAGGA TTAAAAATGC TAAGGCATCC 
GTCAAACTTC GCCGAAATGG GGAACATGTC TTTCTTGTGA CCTATTATTC CTGCCGCGTC 
AACTACAAAC TCAAACGTGC CAAAGATAAT GCACTCTATT GCTCAAATGG TATATGGCTT 
GGTTTGAAAC CCAATTGCAT AAAGATTGGT AAAGGAAACA CGCATTCAAT GAAGCAAAAA 
AAAATGAGAT GCAGGATTGA TAATGGTGGT TGCGCTCATA TATGTAACAG ATCAACTCAT 
AAATGCGAAT GCTATGAGGG TTATACACTG AATTCCACCG ATTTAAGAAG CTGTAATGAT 
ATCGACGAGT GCAAGGAATC GAATGGCGGC TGCTCTCAAG TTTGCAACAA CCTTCCTGGT 
GAATTCATAT GCACCTGTAA TAGTGGTTTT GAAATAGACG AAAGTGATGA AAAAACTTGC 
TTGGACATTG ATGAGTGCGC TGATCCCGAA CTATCGTGGG ACTGTACGGC TGGATGTAAA 
AACCTAAATG GAACATACAA ATGCCTTCCG TCACTGGTGG GCAGAGTTGA GCCCACCGAC 
GGCGATGGTT TCTCCCCGGG TGAAATAGTG TGCAAGTCCG GATTCAAGCT GTCCGACGAT 
GGCAGTGAGT GTCAGGACAT TAACGAATGC GATTTGGCGG ATATTGATTC AGATTCTTGG 
CGAATGACTT ACCGTTACTG TGAGCACAAA TGCGAGAATA CGGTAGGTTC CTATATCTGC 
CATTGCCCCC AAGGATATCA TCTACTCGAT GATCAAAATA GTTGTATTTT GGATGGCTCC 
AAATTACCGC CATCGAAAGA CGCGCCTGTC AGTCAGGAAG CACCAAGAAA GGATATATGC 
CCTTTGTTTA AGGGACCAGC CAACGGAAAG GCTTCTTGCG ATAAGTATCT CCAAAATGGC 
TTCAACTACA CTCGTTGCAA TATTACCTGC AACGCGGGTT ATATCATGCA GGGCTCACAA 
TTTGCGGTTT GTGGACACAC CGGACTTTGG TCTAGTCCAG AAGCCAAGTG CGTGGAATCC 
CTCGCATTGA GTTGTCCAGT TTTGACACCC CCTCGCAATG GACGTTTCTA TCCTGCAAGC 
TGCAATAATG AACCATCCAA ATCACTCGCG ATTTGTGAAC TGTTATGTGA TCGAGGCTAT 
TTGCCCAGAA TGGACACACA GCTCATTTGT GCGGCACCAT GGGGCTGGAT CCAAAGAAGA 
TGGCAGGACA ATGTGCCACA ATATGTGTTT CAGCAATCAG TGAAACAGGA CGAGGGCTGC 
GTGAAGATCG CTCCGCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_729293.1
CDS	446..1906
Translation	MVQNRMSTSVIVLYALMCIAFSTASLNDIPEITPAIDNNLSFQYDDTCWTPPRIKNAKASVKLRRNGEHVFLVTYYSCRVNYKLKRAKDNALYCSNGIWLGLKPNCIKIGKGNTHSMKQKKMRCRIDNGGCAHICNRSTHKCECYEGYTLNSTDLRSCNDIDECKESNGGCSQVCNNLPGEFICTCNSGFEIDESDEKTCLDIDECADPELSWDCTAGCKNLNGTYKCLPSLVGRVEPTDGDGFSPGEIVCKSGFKLSDDGSECQDINECDLADIDSDSWRMTYRYCEHKCENTVGSYICHCPQGYHLLDDQNSCILDGSKLPPSKDAPVSQEAPRKDICPLFKGPANGKASCDKYLQNGFNYTRCNITCNAGYIMQGSQFAVCGHTGLWSSPEAKCVESLALSCPVLTPPRNGRFYPASCNNEPSKSLAICELLCDRGYLPRMDTQLICAAPWGWIQRRWQDNVPQYVFQQSVKQDEGCVKIAPP

Target ORF information:

RefSeq Version	NM_168229.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG32373), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168229.3

ORF Insert Sequence:

ATGGTGCAAA ACAGAATGTC GACATCAGTG ATTGTTTTGT ACGCGCTTAT GTGTATCGCA 
TTTTCCACAG CTTCGTTAAA TGATATTCCC GAAATCACCC CCGCAATAGA TAACAATTTA 
TCTTTTCAAT ACGACGATAC CTGCTGGACT CCTCCGAGGA TTAAAAATGC TAAGGCATCC 
GTCAAACTTC GCCGAAATGG GGAACATGTC TTTCTTGTGA CCTATTATTC CTGCCGCGTC 
AACTACAAAC TCAAACGTGC CAAAGATAAT GCACTCTATT GCTCAAATGG TATATGGCTT 
GGTTTGAAAC CCAATTGCAT AAAGATTGGT AAAGGAAACA CGCATTCAAT GAAGCAAAAA 
AAAATGAGAT GCAGGATTGA TAATGGTGGT TGCGCTCATA TATGTAACAG ATCAACTCAT 
AAATGCGAAT GCTATGAGGG TTATACACTG AATTCCACCG ATTTAAGAAG CTGTAATGAT 
ATCGACGAGT GCAAGGAATC GAATGGCGGC TGCTCTCAAG TTTGCAACAA CCTTCCTGGT 
GAATTCATAT GCACCTGTAA TAGTGGTTTT GAAATAGACG AAAGTGATGA AAAAACTTGC 
TTGGACATTG ATGAGTGCGC TGATCCCGAA CTATCGTGGG ACTGTACGGC TGGATGTAAA 
AACCTAAATG GAACATACAA ATGCCTTCCG TCACTGGTGG GCAGAGTTGA GCCCACCGAC 
GGCGATGGTT TCTCCCCGGG TGAAATAGTG TGCAAGTCCG GATTCAAGCT GTCCGACGAT 
GGCAGTGAGT GTCAGGACAT TAACGAATGC GATTTGGCGG ATATTGATTC AGATTCTTGG 
CGAATGACTT ACCGTTACTG TGAGCACAAA TGCGAGAATA CGGTAGGTTC CTATATCTGC 
CATTGCCCCC AAGGATATCA TCTACTCGAT GATCAAAATA GTTGTATTTT GGATGGCTCC 
AAATTACCGC CATCGAAAGA CGCGCCTGTC AGTCAGGAAG CACCAAGAAA GGATATATGC 
CCTTTGTTTA AGGGACCAGC CAACGGAAAG GCTTCTTGCG ATAAGTATCT CCAAAATGGC 
TTCAACTACA CTCGTTGCAA TATTACCTGC AACGCGGGTT ATATCATGCA GGGCTCACAA 
TTTGCGGTTT GTGGACACAC CGGACTTTGG TCTAGTCCAG AAGCCAAGTG CGTGGAATCC 
CTCGCATTGA GTTGTCCAGT TTTGACACCC CCTCGCAATG GACGTTTCTA TCCTGCAAGC 
TGCAATAATG AACCATCCAA ATCACTCGCG ATTTGTGAAC TGTTATGTGA TCGAGGCTAT 
TTGCCCAGAA TGGACACACA GCTCATTTGT GCGGCACCAT GGGGCTGGAT CCAAAGAAGA 
TGGCAGGACA ATGTGCCACA ATATGTGTTT CAGCAATCAG TGAAACAGGA CGAGGGCTGC 
GTGAAGATCG CTCCGCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG32373 ( NM_206297.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996019.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG32373), mRNA.

Price & Availability↑

CloneID	OFa19541
Clone ID Related Accession (Same CDS sequence)	NM_168229.3 , NM_206297.3 , NM_001274605.1
Accession Version	NM_206297.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1461bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_206297.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGCAAA ACAGAATGTC GACATCAGTG ATTGTTTTGT ACGCGCTTAT GTGTATCGCA 
TTTTCCACAG CTTCGTTAAA TGATATTCCC GAAATCACCC CCGCAATAGA TAACAATTTA 
TCTTTTCAAT ACGACGATAC CTGCTGGACT CCTCCGAGGA TTAAAAATGC TAAGGCATCC 
GTCAAACTTC GCCGAAATGG GGAACATGTC TTTCTTGTGA CCTATTATTC CTGCCGCGTC 
AACTACAAAC TCAAACGTGC CAAAGATAAT GCACTCTATT GCTCAAATGG TATATGGCTT 
GGTTTGAAAC CCAATTGCAT AAAGATTGGT AAAGGAAACA CGCATTCAAT GAAGCAAAAA 
AAAATGAGAT GCAGGATTGA TAATGGTGGT TGCGCTCATA TATGTAACAG ATCAACTCAT 
AAATGCGAAT GCTATGAGGG TTATACACTG AATTCCACCG ATTTAAGAAG CTGTAATGAT 
ATCGACGAGT GCAAGGAATC GAATGGCGGC TGCTCTCAAG TTTGCAACAA CCTTCCTGGT 
GAATTCATAT GCACCTGTAA TAGTGGTTTT GAAATAGACG AAAGTGATGA AAAAACTTGC 
TTGGACATTG ATGAGTGCGC TGATCCCGAA CTATCGTGGG ACTGTACGGC TGGATGTAAA 
AACCTAAATG GAACATACAA ATGCCTTCCG TCACTGGTGG GCAGAGTTGA GCCCACCGAC 
GGCGATGGTT TCTCCCCGGG TGAAATAGTG TGCAAGTCCG GATTCAAGCT GTCCGACGAT 
GGCAGTGAGT GTCAGGACAT TAACGAATGC GATTTGGCGG ATATTGATTC AGATTCTTGG 
CGAATGACTT ACCGTTACTG TGAGCACAAA TGCGAGAATA CGGTAGGTTC CTATATCTGC 
CATTGCCCCC AAGGATATCA TCTACTCGAT GATCAAAATA GTTGTATTTT GGATGGCTCC 
AAATTACCGC CATCGAAAGA CGCGCCTGTC AGTCAGGAAG CACCAAGAAA GGATATATGC 
CCTTTGTTTA AGGGACCAGC CAACGGAAAG GCTTCTTGCG ATAAGTATCT CCAAAATGGC 
TTCAACTACA CTCGTTGCAA TATTACCTGC AACGCGGGTT ATATCATGCA GGGCTCACAA 
TTTGCGGTTT GTGGACACAC CGGACTTTGG TCTAGTCCAG AAGCCAAGTG CGTGGAATCC 
CTCGCATTGA GTTGTCCAGT TTTGACACCC CCTCGCAATG GACGTTTCTA TCCTGCAAGC 
TGCAATAATG AACCATCCAA ATCACTCGCG ATTTGTGAAC TGTTATGTGA TCGAGGCTAT 
TTGCCCAGAA TGGACACACA GCTCATTTGT GCGGCACCAT GGGGCTGGAT CCAAAGAAGA 
TGGCAGGACA ATGTGCCACA ATATGTGTTT CAGCAATCAG TGAAACAGGA CGAGGGCTGC 
GTGAAGATCG CTCCGCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996019.1
CDS	533..1993
Translation	MVQNRMSTSVIVLYALMCIAFSTASLNDIPEITPAIDNNLSFQYDDTCWTPPRIKNAKASVKLRRNGEHVFLVTYYSCRVNYKLKRAKDNALYCSNGIWLGLKPNCIKIGKGNTHSMKQKKMRCRIDNGGCAHICNRSTHKCECYEGYTLNSTDLRSCNDIDECKESNGGCSQVCNNLPGEFICTCNSGFEIDESDEKTCLDIDECADPELSWDCTAGCKNLNGTYKCLPSLVGRVEPTDGDGFSPGEIVCKSGFKLSDDGSECQDINECDLADIDSDSWRMTYRYCEHKCENTVGSYICHCPQGYHLLDDQNSCILDGSKLPPSKDAPVSQEAPRKDICPLFKGPANGKASCDKYLQNGFNYTRCNITCNAGYIMQGSQFAVCGHTGLWSSPEAKCVESLALSCPVLTPPRNGRFYPASCNNEPSKSLAICELLCDRGYLPRMDTQLICAAPWGWIQRRWQDNVPQYVFQQSVKQDEGCVKIAPP

Target ORF information:

RefSeq Version	NM_206297.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG32373), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206297.3

ORF Insert Sequence:

ATGGTGCAAA ACAGAATGTC GACATCAGTG ATTGTTTTGT ACGCGCTTAT GTGTATCGCA 
TTTTCCACAG CTTCGTTAAA TGATATTCCC GAAATCACCC CCGCAATAGA TAACAATTTA 
TCTTTTCAAT ACGACGATAC CTGCTGGACT CCTCCGAGGA TTAAAAATGC TAAGGCATCC 
GTCAAACTTC GCCGAAATGG GGAACATGTC TTTCTTGTGA CCTATTATTC CTGCCGCGTC 
AACTACAAAC TCAAACGTGC CAAAGATAAT GCACTCTATT GCTCAAATGG TATATGGCTT 
GGTTTGAAAC CCAATTGCAT AAAGATTGGT AAAGGAAACA CGCATTCAAT GAAGCAAAAA 
AAAATGAGAT GCAGGATTGA TAATGGTGGT TGCGCTCATA TATGTAACAG ATCAACTCAT 
AAATGCGAAT GCTATGAGGG TTATACACTG AATTCCACCG ATTTAAGAAG CTGTAATGAT 
ATCGACGAGT GCAAGGAATC GAATGGCGGC TGCTCTCAAG TTTGCAACAA CCTTCCTGGT 
GAATTCATAT GCACCTGTAA TAGTGGTTTT GAAATAGACG AAAGTGATGA AAAAACTTGC 
TTGGACATTG ATGAGTGCGC TGATCCCGAA CTATCGTGGG ACTGTACGGC TGGATGTAAA 
AACCTAAATG GAACATACAA ATGCCTTCCG TCACTGGTGG GCAGAGTTGA GCCCACCGAC 
GGCGATGGTT TCTCCCCGGG TGAAATAGTG TGCAAGTCCG GATTCAAGCT GTCCGACGAT 
GGCAGTGAGT GTCAGGACAT TAACGAATGC GATTTGGCGG ATATTGATTC AGATTCTTGG 
CGAATGACTT ACCGTTACTG TGAGCACAAA TGCGAGAATA CGGTAGGTTC CTATATCTGC 
CATTGCCCCC AAGGATATCA TCTACTCGAT GATCAAAATA GTTGTATTTT GGATGGCTCC 
AAATTACCGC CATCGAAAGA CGCGCCTGTC AGTCAGGAAG CACCAAGAAA GGATATATGC 
CCTTTGTTTA AGGGACCAGC CAACGGAAAG GCTTCTTGCG ATAAGTATCT CCAAAATGGC 
TTCAACTACA CTCGTTGCAA TATTACCTGC AACGCGGGTT ATATCATGCA GGGCTCACAA 
TTTGCGGTTT GTGGACACAC CGGACTTTGG TCTAGTCCAG AAGCCAAGTG CGTGGAATCC 
CTCGCATTGA GTTGTCCAGT TTTGACACCC CCTCGCAATG GACGTTTCTA TCCTGCAAGC 
TGCAATAATG AACCATCCAA ATCACTCGCG ATTTGTGAAC TGTTATGTGA TCGAGGCTAT 
TTGCCCAGAA TGGACACACA GCTCATTTGT GCGGCACCAT GGGGCTGGAT CCAAAGAAGA 
TGGCAGGACA ATGTGCCACA ATATGTGTTT CAGCAATCAG TGAAACAGGA CGAGGGCTGC 
GTGAAGATCG CTCCGCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG32373 ( NM_001274605.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261534.1 Drosophila melanogaster uncharacterized protein, transcript variant C (CG32373), mRNA.

Price & Availability↑

CloneID	OFa19541
Clone ID Related Accession (Same CDS sequence)	NM_168229.3 , NM_206297.3 , NM_001274605.1
Accession Version	NM_001274605.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1461bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTGCAAA ACAGAATGTC GACATCAGTG ATTGTTTTGT ACGCGCTTAT GTGTATCGCA 
TTTTCCACAG CTTCGTTAAA TGATATTCCC GAAATCACCC CCGCAATAGA TAACAATTTA 
TCTTTTCAAT ACGACGATAC CTGCTGGACT CCTCCGAGGA TTAAAAATGC TAAGGCATCC 
GTCAAACTTC GCCGAAATGG GGAACATGTC TTTCTTGTGA CCTATTATTC CTGCCGCGTC 
AACTACAAAC TCAAACGTGC CAAAGATAAT GCACTCTATT GCTCAAATGG TATATGGCTT 
GGTTTGAAAC CCAATTGCAT AAAGATTGGT AAAGGAAACA CGCATTCAAT GAAGCAAAAA 
AAAATGAGAT GCAGGATTGA TAATGGTGGT TGCGCTCATA TATGTAACAG ATCAACTCAT 
AAATGCGAAT GCTATGAGGG TTATACACTG AATTCCACCG ATTTAAGAAG CTGTAATGAT 
ATCGACGAGT GCAAGGAATC GAATGGCGGC TGCTCTCAAG TTTGCAACAA CCTTCCTGGT 
GAATTCATAT GCACCTGTAA TAGTGGTTTT GAAATAGACG AAAGTGATGA AAAAACTTGC 
TTGGACATTG ATGAGTGCGC TGATCCCGAA CTATCGTGGG ACTGTACGGC TGGATGTAAA 
AACCTAAATG GAACATACAA ATGCCTTCCG TCACTGGTGG GCAGAGTTGA GCCCACCGAC 
GGCGATGGTT TCTCCCCGGG TGAAATAGTG TGCAAGTCCG GATTCAAGCT GTCCGACGAT 
GGCAGTGAGT GTCAGGACAT TAACGAATGC GATTTGGCGG ATATTGATTC AGATTCTTGG 
CGAATGACTT ACCGTTACTG TGAGCACAAA TGCGAGAATA CGGTAGGTTC CTATATCTGC 
CATTGCCCCC AAGGATATCA TCTACTCGAT GATCAAAATA GTTGTATTTT GGATGGCTCC 
AAATTACCGC CATCGAAAGA CGCGCCTGTC AGTCAGGAAG CACCAAGAAA GGATATATGC 
CCTTTGTTTA AGGGACCAGC CAACGGAAAG GCTTCTTGCG ATAAGTATCT CCAAAATGGC 
TTCAACTACA CTCGTTGCAA TATTACCTGC AACGCGGGTT ATATCATGCA GGGCTCACAA 
TTTGCGGTTT GTGGACACAC CGGACTTTGG TCTAGTCCAG AAGCCAAGTG CGTGGAATCC 
CTCGCATTGA GTTGTCCAGT TTTGACACCC CCTCGCAATG GACGTTTCTA TCCTGCAAGC 
TGCAATAATG AACCATCCAA ATCACTCGCG ATTTGTGAAC TGTTATGTGA TCGAGGCTAT 
TTGCCCAGAA TGGACACACA GCTCATTTGT GCGGCACCAT GGGGCTGGAT CCAAAGAAGA 
TGGCAGGACA ATGTGCCACA ATATGTGTTT CAGCAATCAG TGAAACAGGA CGAGGGCTGC 
GTGAAGATCG CTCCGCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261534.1
CDS	279..1739
Translation	MVQNRMSTSVIVLYALMCIAFSTASLNDIPEITPAIDNNLSFQYDDTCWTPPRIKNAKASVKLRRNGEHVFLVTYYSCRVNYKLKRAKDNALYCSNGIWLGLKPNCIKIGKGNTHSMKQKKMRCRIDNGGCAHICNRSTHKCECYEGYTLNSTDLRSCNDIDECKESNGGCSQVCNNLPGEFICTCNSGFEIDESDEKTCLDIDECADPELSWDCTAGCKNLNGTYKCLPSLVGRVEPTDGDGFSPGEIVCKSGFKLSDDGSECQDINECDLADIDSDSWRMTYRYCEHKCENTVGSYICHCPQGYHLLDDQNSCILDGSKLPPSKDAPVSQEAPRKDICPLFKGPANGKASCDKYLQNGFNYTRCNITCNAGYIMQGSQFAVCGHTGLWSSPEAKCVESLALSCPVLTPPRNGRFYPASCNNEPSKSLAICELLCDRGYLPRMDTQLICAAPWGWIQRRWQDNVPQYVFQQSVKQDEGCVKIAPP

Target ORF information:

RefSeq Version	NM_001274605.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant C (CG32373), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274605.1

ORF Insert Sequence:

ATGGTGCAAA ACAGAATGTC GACATCAGTG ATTGTTTTGT ACGCGCTTAT GTGTATCGCA 
TTTTCCACAG CTTCGTTAAA TGATATTCCC GAAATCACCC CCGCAATAGA TAACAATTTA 
TCTTTTCAAT ACGACGATAC CTGCTGGACT CCTCCGAGGA TTAAAAATGC TAAGGCATCC 
GTCAAACTTC GCCGAAATGG GGAACATGTC TTTCTTGTGA CCTATTATTC CTGCCGCGTC 
AACTACAAAC TCAAACGTGC CAAAGATAAT GCACTCTATT GCTCAAATGG TATATGGCTT 
GGTTTGAAAC CCAATTGCAT AAAGATTGGT AAAGGAAACA CGCATTCAAT GAAGCAAAAA 
AAAATGAGAT GCAGGATTGA TAATGGTGGT TGCGCTCATA TATGTAACAG ATCAACTCAT 
AAATGCGAAT GCTATGAGGG TTATACACTG AATTCCACCG ATTTAAGAAG CTGTAATGAT 
ATCGACGAGT GCAAGGAATC GAATGGCGGC TGCTCTCAAG TTTGCAACAA CCTTCCTGGT 
GAATTCATAT GCACCTGTAA TAGTGGTTTT GAAATAGACG AAAGTGATGA AAAAACTTGC 
TTGGACATTG ATGAGTGCGC TGATCCCGAA CTATCGTGGG ACTGTACGGC TGGATGTAAA 
AACCTAAATG GAACATACAA ATGCCTTCCG TCACTGGTGG GCAGAGTTGA GCCCACCGAC 
GGCGATGGTT TCTCCCCGGG TGAAATAGTG TGCAAGTCCG GATTCAAGCT GTCCGACGAT 
GGCAGTGAGT GTCAGGACAT TAACGAATGC GATTTGGCGG ATATTGATTC AGATTCTTGG 
CGAATGACTT ACCGTTACTG TGAGCACAAA TGCGAGAATA CGGTAGGTTC CTATATCTGC 
CATTGCCCCC AAGGATATCA TCTACTCGAT GATCAAAATA GTTGTATTTT GGATGGCTCC 
AAATTACCGC CATCGAAAGA CGCGCCTGTC AGTCAGGAAG CACCAAGAAA GGATATATGC 
CCTTTGTTTA AGGGACCAGC CAACGGAAAG GCTTCTTGCG ATAAGTATCT CCAAAATGGC 
TTCAACTACA CTCGTTGCAA TATTACCTGC AACGCGGGTT ATATCATGCA GGGCTCACAA 
TTTGCGGTTT GTGGACACAC CGGACTTTGG TCTAGTCCAG AAGCCAAGTG CGTGGAATCC 
CTCGCATTGA GTTGTCCAGT TTTGACACCC CCTCGCAATG GACGTTTCTA TCCTGCAAGC 
TGCAATAATG AACCATCCAA ATCACTCGCG ATTTGTGAAC TGTTATGTGA TCGAGGCTAT 
TTGCCCAGAA TGGACACACA GCTCATTTGT GCGGCACCAT GGGGCTGGAT CCAAAGAAGA 
TGGCAGGACA ATGTGCCACA ATATGTGTTT CAGCAATCAG TGAAACAGGA CGAGGGCTGC 
GTGAAGATCG CTCCGCCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG32373 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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