Trf4-1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Trf4-1
Entrez Gene ID	31795
Full Name	Topoisomerase related function 4-1
Synonyms	AAF46390,CG11265,DmTRF4-1,Dmel\CG11265,Trf4,dTrf4-1
General protein information	Preferred Names Topoisomerase related function 4-1 Names CG11265 gene product from transcript CG11265-RE CG11265-PC CG11265-PE CG11265-PF CG11265-PG CG11265-PH CG11265-PI CG11265-PJ Trf4-1-PC Trf4-1-PE Trf4-1-PF Trf4-1-PG Trf4-1-PH Trf4-1-PI Trf4-1-PJ topoisomerase related function 4-1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 7F4-7F4

mRNA and Protein(s)

mRNA	Protein	Name
NM_206659.3	NP_996382.2	topoisomerase related function 4-1, isoform C
NM_206658.4	NP_996381.1	topoisomerase related function 4-1, isoform E
NM_001272411.1	NP_001259340.1	topoisomerase related function 4-1, isoform J
NM_001103443.2	NP_001096913.1	topoisomerase related function 4-1, isoform F
NM_132262.3	NP_572490.2	topoisomerase related function 4-1, isoform I
NM_206660.2	NP_996383.2	topoisomerase related function 4-1, isoform H
NM_001103444.2	NP_001096914.1	topoisomerase related function 4-1, isoform G

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest that DmTRF4-1 (CG11265) and DmRrp6 are involved in the polyadenylation-mediated degradation of snRNAs in vivo.
Title: TRF4 is involved in polyadenylation of snRNAs in Drosophila melanogaster.

The following Trf4-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Trf4-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01133	NM_206658.4 Latest version!	Drosophila melanogaster topoisomerase related function 4-1, transcript variant E (Trf4-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa01133	NM_206659.3 Latest version!	Drosophila melanogaster topoisomerase related function 4-1, transcript variant C (Trf4-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa01133	NM_206660.2 Latest version!	Drosophila melanogaster topoisomerase related function 4-1, transcript variant H (Trf4-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa01133	NM_001272411.1 Latest version!	Drosophila melanogaster topoisomerase related function 4-1, transcript variant J (Trf4-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa01133	NM_132262.3 Latest version!	Drosophila melanogaster topoisomerase related function 4-1, transcript variant I (Trf4-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OFa01134	NM_001103443.2 Latest version!	Drosophila melanogaster topoisomerase related function 4-1, transcript variant F (Trf4-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
OFa01134	NM_001103444.2 Latest version!	Drosophila melanogaster topoisomerase related function 4-1, transcript variant G (Trf4-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Trf4-1 ( NM_206659.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996382.2 Drosophila melanogaster topoisomerase related function 4-1, transcript variant C (Trf4-1), mRNA.

Price & Availability↑

CloneID	OFa01133
Clone ID Related Accession (Same CDS sequence)	NM_206659.3 , NM_206658.4 , NM_001272411.1 , NM_132262.3 , NM_206660.2
Accession Version	NM_206659.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3006bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	topoisomerase related function 4-1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_206659.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCCAT TTGTTGGCTG GTTTCAGAAG GAACAGGAGG GACCATCGCT GTGCACATGG 
TTAAAGATTT GGGAGACGAA TGCCCAAGAG ATGGGCGCAT TGCTTAATCA ACAGCTACAG 
CAGGCGACGA CGATCAACGG GAGCACGAGC AGCAGCAGCA GCAGCAGCAA TAGTGGTAAC 
AACAACAACA ACAACAACAA TAACATCATC AATAACACTA TCACCAACAC GACCAATAAC 
ACTGGTAACA ATTCATCGGC CAAACCCTAC TTATCGCGAC CCTACAGCTC GCTAAATCGG 
GTGCTCAATT TCCGAGCGGA TTCCCTGGAG ATCCTGCAGC AGCAACAACA ACAGCAGCAG 
CTCAACGGGA CCACTCAGCG CAACAGCACC AACATCAATA CCACAAGCGG CGGCAGCACT 
AGCTCGTCGG CAGATTCCAC CACGAATCGG GACAATAACT CACCCGCCAA TAGCAGTTCA 
ACCAACGGAC CCGGAGCAGG AACGGGAACG TCAACAGGAG CAGGAGGTAC CGGTACAAAT 
AGTCCGGCGA CGACAGCGTC AAGCACAGCT GCGACCACGA CTGGTCCAGC CACTAGTATG 
AGCGACACCA GCAACAACCC GCCACAATCA ACGACCACGC CCGCCAGCCG CACGAACAGC 
ATCTACTACA ATCCATCGCG GAAGAAACGA CCGGAGAACA AGGCTGGCGG AGCCCACTAC 
TATATGAACA ACCATATGGA GATGATCGCC AAGTACAAGG GCGAACCGTG GCGCAAGCCG 
GACTATCCGT ATGGCGAGGG CGTTATCGGG CTGCACGAGG AGATCGAGCA TTTCTATCAG 
TACGTCCTGC CCACGCCCTG CGAACACGCC ATCCGCAACG AGGTGGTCAA GCGTATCGAG 
GCCGTTGTCC ATTCCATCTG GCCACAGGCA GTAGTCGAGA TCTTTGGCTC CTTTCGCACT 
GGCCTCTTTT TGCCCACCTC CGACATAGAT CTCGTTGTGT TAGGTTTGTG GGAGAAGCTG 
CCGCTGAGAA CGCTGGAGTT CGAGCTAGTG AGCCGTGGTA TCGCCGAGGC TTGTACGGTG 
CGTGTTCTGG ACAAGGCATC AGTGCCCATC ATCAAGCTGA CGGACCGGGA AACGCAAGTG 
AAGGTAGACA TATCGTTCAA CATGCAGAGT GGTGTTCAGT CGGCGGAGCT GATTAAGAAG 
TTTAAGCGCG ACTATCCAGT GCTGGAGAAG CTAGTGCTTG TGCTGAAGCA GTTCTTACTA 
CTTCGCGATC TCAACGAGGT GTTCACGGGC GGCATCTCCT CCTACTCGCT GATCCTTATG 
TGCATCAGCT TCCTGCAGAT GCATCCGCGC GGTATCTACC ACGATACAGC CAATCTGGGG 
GTGCTTCTCC TCGAGTTCTT CGAACTTTAT GGACGTCGCT TCAACTACAT GAAGATCGGC 
ATCTCCATAA AGAACGGTGG CCGCTACATG CCCAAGGATG AGCTGCAGCG TGACATGGTC 
GATGGCCATC GACCCTCGCT CCTGTGCATC GAGGATCCGC TGACGCCAGG CAATGATATC 
GGTCGAAGCA GCTACGGCGT CTTCCAGGTG CAACAGGCAT TCAAGTGCGC CTACCGCGTG 
CTCGCCCTTG CCGTCAGTCC GCTCAACCTT CTCGGCATCG ATCCGCGCGT CAACTCGATA 
CTGGGTCGAA TCATTCACAT CACCGACGAC GTGATCGATT ATCGCGAATG GATCCGCGAG 
AACTTCGAAC ATCTGGTGGT GGTGGATCGT ATCTCGCCGC TGCCCACTGC CGCTCCCACT 
GCGTACGCCA CCGCCAATGG GGCGCCAAAA TATGTGATTA TGCCCTCGGG CGCCGTTGTC 
CAGCAGCTGT ACCATCATCC GGTGCAGGTG CCCACCGCTC ACGGGCACAG CCATGCGCAC 
AGCCACAGTC ACGGGCACGC GCATCCTGGC GCCCATCTGT GCCAGCCGTA TGTGACCGGG 
ACCACTGTAT CTGCTGTAAC GACGACTACA ACGATGGCGG TGGTGACCGT GGGCGTGTCA 
GCCGGCGGAG TCCAACAGCA GCAACAGCAA CAGAATGCGA CGGCCCATAC CCACTCGCAA 
CAGCAGACAC AGAACCAATC GCAGTCGCGC CATAGACGCG GCAGCACCTC GTCGGGCGAT 
GACTCCGAGG ATAGCAAGGA CGGCGATGTT GTCGAGACGA CGTCGAGTGC TCAGGAGGTC 
GTTGACATCG CGCTATCGAC GCCAAATGGC CTGGCCAACA TGTCCATGCC CATGCCAGTG 
CATGCTGTCG GAATGCCTGC GTCGAACAGT TGGAGCGGTA ATGGCAATGG AAACGGAAAC 
AGCAGCTCAT CCACGGGCTC GTCGCCGGAA ATCGCACACA TCGCTGCCCA GGAAATGGAC 
CCCGAGCTTG AGGATCAACA GCAACAACAG CAGCACCAAG AGACTTCTGG AGGTAATGGC 
TTCATCCGAC CGGGTGACGT CGGCACCGGT TCCAACAGGG GAGGCGGTGA CGGCAGTGGC 
GGCCGCAATT ACAACCAGCG GAACAACCAC AACTCCAGCG GCTACTACCA CCAGCAGTAC 
TACGTGCCAC CGCCGATGCA ACAGCAGCTG AGCAAGTCCA ACTCCTCCTC CAACTATCAC 
CAGCAACACC ATCACAGCCA CAGCCATGGC AATCATAGTC ACCGACAACA GCATCACCAC 
CAGCAGCAGC ACCACCACCA GCAGCGGCCG CAGCATCTGC GTGTGGGCGG CGGCAATCGA 
TACCAGAAAT CGTTGGGCGG ATCGCCCATC ATCAGTGCTG GCAATGCGTC GAACAGCAGC 
AGTAATTGCA GCAACAGCAG CAGCAGCAGC GGCAGCAACA ACAGCCGACT GCCGCCGCTA 
AGGGGCACGC TGGTCAACTC ATCGTCAGCA ATATCAATCA TTTCCATATC CTCAGAATCA 
TCCATTGCTA GCAGCAGCTC CAGTTCGTCA AGATCCGGTC AGGATCAGCA GCGTGACGAG 
CGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996382.2
CDS	518..3523
Translation	MDPFVGWFQKEQEGPSLCTWLKIWETNAQEMGALLNQQLQQATTINGSTSSSSSSSNSGNNNNNNNNNIINNTITNTTNNTGNNSSAKPYLSRPYSSLNRVLNFRADSLEILQQQQQQQQLNGTTQRNSTNINTTSGGSTSSSADSTTNRDNNSPANSSSTNGPGAGTGTSTGAGGTGTNSPATTASSTAATTTGPATSMSDTSNNPPQSTTTPASRTNSIYYNPSRKKRPENKAGGAHYYMNNHMEMIAKYKGEPWRKPDYPYGEGVIGLHEEIEHFYQYVLPTPCEHAIRNEVVKRIEAVVHSIWPQAVVEIFGSFRTGLFLPTSDIDLVVLGLWEKLPLRTLEFELVSRGIAEACTVRVLDKASVPIIKLTDRETQVKVDISFNMQSGVQSAELIKKFKRDYPVLEKLVLVLKQFLLLRDLNEVFTGGISSYSLILMCISFLQMHPRGIYHDTANLGVLLLEFFELYGRRFNYMKIGISIKNGGRYMPKDELQRDMVDGHRPSLLCIEDPLTPGNDIGRSSYGVFQVQQAFKCAYRVLALAVSPLNLLGIDPRVNSILGRIIHITDDVIDYREWIRENFEHLVVVDRISPLPTAAPTAYATANGAPKYVIMPSGAVVQQLYHHPVQVPTAHGHSHAHSHSHGHAHPGAHLCQPYVTGTTVSAVTTTTTMAVVTVGVSAGGVQQQQQQQNATAHTHSQQQTQNQSQSRHRRGSTSSGDDSEDSKDGDVVETTSSAQEVVDIALSTPNGLANMSMPMPVHAVGMPASNSWSGNGNGNGNSSSSTGSSPEIAHIAAQEMDPELEDQQQQQQHQETSGGNGFIRPGDVGTGSNRGGGDGSGGRNYNQRNNHNSSGYYHQQYYVPPPMQQQLSKSNSSSNYHQQHHHSHSHGNHSHRQQHHHQQQHHHQQRPQHLRVGGGNRYQKSLGGSPIISAGNASNSSSNCSNSSSSSGSNNSRLPPLRGTLVNSSSAISIISISSESSIASSSSSSSRSGQDQQRDER

Target ORF information:

RefSeq Version	NM_206659.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster topoisomerase related function 4-1, transcript variant C (Trf4-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206659.3

ORF Insert Sequence:

ATGGATCCAT TTGTTGGCTG GTTTCAGAAG GAACAGGAGG GACCATCGCT GTGCACATGG 
TTAAAGATTT GGGAGACGAA TGCCCAAGAG ATGGGCGCAT TGCTTAATCA ACAGCTACAG 
CAGGCGACGA CGATCAACGG GAGCACGAGC AGCAGCAGCA GCAGCAGCAA TAGTGGTAAC 
AACAACAACA ACAACAACAA TAACATCATC AATAACACTA TCACCAACAC GACCAATAAC 
ACTGGTAACA ATTCATCGGC CAAACCCTAC TTATCGCGAC CCTACAGCTC GCTAAATCGG 
GTGCTCAATT TCCGAGCGGA TTCCCTGGAG ATCCTGCAGC AGCAACAACA ACAGCAGCAG 
CTCAACGGGA CCACTCAGCG CAACAGCACC AACATCAATA CCACAAGCGG CGGCAGCACT 
AGCTCGTCGG CAGATTCCAC CACGAATCGG GACAATAACT CACCCGCCAA TAGCAGTTCA 
ACCAACGGAC CCGGAGCAGG AACGGGAACG TCAACAGGAG CAGGAGGTAC CGGTACAAAT 
AGTCCGGCGA CGACAGCGTC AAGCACAGCT GCGACCACGA CTGGTCCAGC CACTAGTATG 
AGCGACACCA GCAACAACCC GCCACAATCA ACGACCACGC CCGCCAGCCG CACGAACAGC 
ATCTACTACA ATCCATCGCG GAAGAAACGA CCGGAGAACA AGGCTGGCGG AGCCCACTAC 
TATATGAACA ACCATATGGA GATGATCGCC AAGTACAAGG GCGAACCGTG GCGCAAGCCG 
GACTATCCGT ATGGCGAGGG CGTTATCGGG CTGCACGAGG AGATCGAGCA TTTCTATCAG 
TACGTCCTGC CCACGCCCTG CGAACACGCC ATCCGCAACG AGGTGGTCAA GCGTATCGAG 
GCCGTTGTCC ATTCCATCTG GCCACAGGCA GTAGTCGAGA TCTTTGGCTC CTTTCGCACT 
GGCCTCTTTT TGCCCACCTC CGACATAGAT CTCGTTGTGT TAGGTTTGTG GGAGAAGCTG 
CCGCTGAGAA CGCTGGAGTT CGAGCTAGTG AGCCGTGGTA TCGCCGAGGC TTGTACGGTG 
CGTGTTCTGG ACAAGGCATC AGTGCCCATC ATCAAGCTGA CGGACCGGGA AACGCAAGTG 
AAGGTAGACA TATCGTTCAA CATGCAGAGT GGTGTTCAGT CGGCGGAGCT GATTAAGAAG 
TTTAAGCGCG ACTATCCAGT GCTGGAGAAG CTAGTGCTTG TGCTGAAGCA GTTCTTACTA 
CTTCGCGATC TCAACGAGGT GTTCACGGGC GGCATCTCCT CCTACTCGCT GATCCTTATG 
TGCATCAGCT TCCTGCAGAT GCATCCGCGC GGTATCTACC ACGATACAGC CAATCTGGGG 
GTGCTTCTCC TCGAGTTCTT CGAACTTTAT GGACGTCGCT TCAACTACAT GAAGATCGGC 
ATCTCCATAA AGAACGGTGG CCGCTACATG CCCAAGGATG AGCTGCAGCG TGACATGGTC 
GATGGCCATC GACCCTCGCT CCTGTGCATC GAGGATCCGC TGACGCCAGG CAATGATATC 
GGTCGAAGCA GCTACGGCGT CTTCCAGGTG CAACAGGCAT TCAAGTGCGC CTACCGCGTG 
CTCGCCCTTG CCGTCAGTCC GCTCAACCTT CTCGGCATCG ATCCGCGCGT CAACTCGATA 
CTGGGTCGAA TCATTCACAT CACCGACGAC GTGATCGATT ATCGCGAATG GATCCGCGAG 
AACTTCGAAC ATCTGGTGGT GGTGGATCGT ATCTCGCCGC TGCCCACTGC CGCTCCCACT 
GCGTACGCCA CCGCCAATGG GGCGCCAAAA TATGTGATTA TGCCCTCGGG CGCCGTTGTC 
CAGCAGCTGT ACCATCATCC GGTGCAGGTG CCCACCGCTC ACGGGCACAG CCATGCGCAC 
AGCCACAGTC ACGGGCACGC GCATCCTGGC GCCCATCTGT GCCAGCCGTA TGTGACCGGG 
ACCACTGTAT CTGCTGTAAC GACGACTACA ACGATGGCGG TGGTGACCGT GGGCGTGTCA 
GCCGGCGGAG TCCAACAGCA GCAACAGCAA CAGAATGCGA CGGCCCATAC CCACTCGCAA 
CAGCAGACAC AGAACCAATC GCAGTCGCGC CATAGACGCG GCAGCACCTC GTCGGGCGAT 
GACTCCGAGG ATAGCAAGGA CGGCGATGTT GTCGAGACGA CGTCGAGTGC TCAGGAGGTC 
GTTGACATCG CGCTATCGAC GCCAAATGGC CTGGCCAACA TGTCCATGCC CATGCCAGTG 
CATGCTGTCG GAATGCCTGC GTCGAACAGT TGGAGCGGTA ATGGCAATGG AAACGGAAAC 
AGCAGCTCAT CCACGGGCTC GTCGCCGGAA ATCGCACACA TCGCTGCCCA GGAAATGGAC 
CCCGAGCTTG AGGATCAACA GCAACAACAG CAGCACCAAG AGACTTCTGG AGGTAATGGC 
TTCATCCGAC CGGGTGACGT CGGCACCGGT TCCAACAGGG GAGGCGGTGA CGGCAGTGGC 
GGCCGCAATT ACAACCAGCG GAACAACCAC AACTCCAGCG GCTACTACCA CCAGCAGTAC 
TACGTGCCAC CGCCGATGCA ACAGCAGCTG AGCAAGTCCA ACTCCTCCTC CAACTATCAC 
CAGCAACACC ATCACAGCCA CAGCCATGGC AATCATAGTC ACCGACAACA GCATCACCAC 
CAGCAGCAGC ACCACCACCA GCAGCGGCCG CAGCATCTGC GTGTGGGCGG CGGCAATCGA 
TACCAGAAAT CGTTGGGCGG ATCGCCCATC ATCAGTGCTG GCAATGCGTC GAACAGCAGC 
AGTAATTGCA GCAACAGCAG CAGCAGCAGC GGCAGCAACA ACAGCCGACT GCCGCCGCTA 
AGGGGCACGC TGGTCAACTC ATCGTCAGCA ATATCAATCA TTTCCATATC CTCAGAATCA 
TCCATTGCTA GCAGCAGCTC CAGTTCGTCA AGATCCGGTC AGGATCAGCA GCGTGACGAG 
CGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trf4-1 ( NM_206658.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996381.1 Drosophila melanogaster topoisomerase related function 4-1, transcript variant E (Trf4-1), mRNA.

Price & Availability↑

CloneID	OFa01133
Clone ID Related Accession (Same CDS sequence)	NM_206659.3 , NM_206658.4 , NM_001272411.1 , NM_132262.3 , NM_206660.2
Accession Version	NM_206658.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3006bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	topoisomerase related function 4-1, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_206658.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCCAT TTGTTGGCTG GTTTCAGAAG GAACAGGAGG GACCATCGCT GTGCACATGG 
TTAAAGATTT GGGAGACGAA TGCCCAAGAG ATGGGCGCAT TGCTTAATCA ACAGCTACAG 
CAGGCGACGA CGATCAACGG GAGCACGAGC AGCAGCAGCA GCAGCAGCAA TAGTGGTAAC 
AACAACAACA ACAACAACAA TAACATCATC AATAACACTA TCACCAACAC GACCAATAAC 
ACTGGTAACA ATTCATCGGC CAAACCCTAC TTATCGCGAC CCTACAGCTC GCTAAATCGG 
GTGCTCAATT TCCGAGCGGA TTCCCTGGAG ATCCTGCAGC AGCAACAACA ACAGCAGCAG 
CTCAACGGGA CCACTCAGCG CAACAGCACC AACATCAATA CCACAAGCGG CGGCAGCACT 
AGCTCGTCGG CAGATTCCAC CACGAATCGG GACAATAACT CACCCGCCAA TAGCAGTTCA 
ACCAACGGAC CCGGAGCAGG AACGGGAACG TCAACAGGAG CAGGAGGTAC CGGTACAAAT 
AGTCCGGCGA CGACAGCGTC AAGCACAGCT GCGACCACGA CTGGTCCAGC CACTAGTATG 
AGCGACACCA GCAACAACCC GCCACAATCA ACGACCACGC CCGCCAGCCG CACGAACAGC 
ATCTACTACA ATCCATCGCG GAAGAAACGA CCGGAGAACA AGGCTGGCGG AGCCCACTAC 
TATATGAACA ACCATATGGA GATGATCGCC AAGTACAAGG GCGAACCGTG GCGCAAGCCG 
GACTATCCGT ATGGCGAGGG CGTTATCGGG CTGCACGAGG AGATCGAGCA TTTCTATCAG 
TACGTCCTGC CCACGCCCTG CGAACACGCC ATCCGCAACG AGGTGGTCAA GCGTATCGAG 
GCCGTTGTCC ATTCCATCTG GCCACAGGCA GTAGTCGAGA TCTTTGGCTC CTTTCGCACT 
GGCCTCTTTT TGCCCACCTC CGACATAGAT CTCGTTGTGT TAGGTTTGTG GGAGAAGCTG 
CCGCTGAGAA CGCTGGAGTT CGAGCTAGTG AGCCGTGGTA TCGCCGAGGC TTGTACGGTG 
CGTGTTCTGG ACAAGGCATC AGTGCCCATC ATCAAGCTGA CGGACCGGGA AACGCAAGTG 
AAGGTAGACA TATCGTTCAA CATGCAGAGT GGTGTTCAGT CGGCGGAGCT GATTAAGAAG 
TTTAAGCGCG ACTATCCAGT GCTGGAGAAG CTAGTGCTTG TGCTGAAGCA GTTCTTACTA 
CTTCGCGATC TCAACGAGGT GTTCACGGGC GGCATCTCCT CCTACTCGCT GATCCTTATG 
TGCATCAGCT TCCTGCAGAT GCATCCGCGC GGTATCTACC ACGATACAGC CAATCTGGGG 
GTGCTTCTCC TCGAGTTCTT CGAACTTTAT GGACGTCGCT TCAACTACAT GAAGATCGGC 
ATCTCCATAA AGAACGGTGG CCGCTACATG CCCAAGGATG AGCTGCAGCG TGACATGGTC 
GATGGCCATC GACCCTCGCT CCTGTGCATC GAGGATCCGC TGACGCCAGG CAATGATATC 
GGTCGAAGCA GCTACGGCGT CTTCCAGGTG CAACAGGCAT TCAAGTGCGC CTACCGCGTG 
CTCGCCCTTG CCGTCAGTCC GCTCAACCTT CTCGGCATCG ATCCGCGCGT CAACTCGATA 
CTGGGTCGAA TCATTCACAT CACCGACGAC GTGATCGATT ATCGCGAATG GATCCGCGAG 
AACTTCGAAC ATCTGGTGGT GGTGGATCGT ATCTCGCCGC TGCCCACTGC CGCTCCCACT 
GCGTACGCCA CCGCCAATGG GGCGCCAAAA TATGTGATTA TGCCCTCGGG CGCCGTTGTC 
CAGCAGCTGT ACCATCATCC GGTGCAGGTG CCCACCGCTC ACGGGCACAG CCATGCGCAC 
AGCCACAGTC ACGGGCACGC GCATCCTGGC GCCCATCTGT GCCAGCCGTA TGTGACCGGG 
ACCACTGTAT CTGCTGTAAC GACGACTACA ACGATGGCGG TGGTGACCGT GGGCGTGTCA 
GCCGGCGGAG TCCAACAGCA GCAACAGCAA CAGAATGCGA CGGCCCATAC CCACTCGCAA 
CAGCAGACAC AGAACCAATC GCAGTCGCGC CATAGACGCG GCAGCACCTC GTCGGGCGAT 
GACTCCGAGG ATAGCAAGGA CGGCGATGTT GTCGAGACGA CGTCGAGTGC TCAGGAGGTC 
GTTGACATCG CGCTATCGAC GCCAAATGGC CTGGCCAACA TGTCCATGCC CATGCCAGTG 
CATGCTGTCG GAATGCCTGC GTCGAACAGT TGGAGCGGTA ATGGCAATGG AAACGGAAAC 
AGCAGCTCAT CCACGGGCTC GTCGCCGGAA ATCGCACACA TCGCTGCCCA GGAAATGGAC 
CCCGAGCTTG AGGATCAACA GCAACAACAG CAGCACCAAG AGACTTCTGG AGGTAATGGC 
TTCATCCGAC CGGGTGACGT CGGCACCGGT TCCAACAGGG GAGGCGGTGA CGGCAGTGGC 
GGCCGCAATT ACAACCAGCG GAACAACCAC AACTCCAGCG GCTACTACCA CCAGCAGTAC 
TACGTGCCAC CGCCGATGCA ACAGCAGCTG AGCAAGTCCA ACTCCTCCTC CAACTATCAC 
CAGCAACACC ATCACAGCCA CAGCCATGGC AATCATAGTC ACCGACAACA GCATCACCAC 
CAGCAGCAGC ACCACCACCA GCAGCGGCCG CAGCATCTGC GTGTGGGCGG CGGCAATCGA 
TACCAGAAAT CGTTGGGCGG ATCGCCCATC ATCAGTGCTG GCAATGCGTC GAACAGCAGC 
AGTAATTGCA GCAACAGCAG CAGCAGCAGC GGCAGCAACA ACAGCCGACT GCCGCCGCTA 
AGGGGCACGC TGGTCAACTC ATCGTCAGCA ATATCAATCA TTTCCATATC CTCAGAATCA 
TCCATTGCTA GCAGCAGCTC CAGTTCGTCA AGATCCGGTC AGGATCAGCA GCGTGACGAG 
CGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996381.1
CDS	713..3718
Translation	MDPFVGWFQKEQEGPSLCTWLKIWETNAQEMGALLNQQLQQATTINGSTSSSSSSSNSGNNNNNNNNNIINNTITNTTNNTGNNSSAKPYLSRPYSSLNRVLNFRADSLEILQQQQQQQQLNGTTQRNSTNINTTSGGSTSSSADSTTNRDNNSPANSSSTNGPGAGTGTSTGAGGTGTNSPATTASSTAATTTGPATSMSDTSNNPPQSTTTPASRTNSIYYNPSRKKRPENKAGGAHYYMNNHMEMIAKYKGEPWRKPDYPYGEGVIGLHEEIEHFYQYVLPTPCEHAIRNEVVKRIEAVVHSIWPQAVVEIFGSFRTGLFLPTSDIDLVVLGLWEKLPLRTLEFELVSRGIAEACTVRVLDKASVPIIKLTDRETQVKVDISFNMQSGVQSAELIKKFKRDYPVLEKLVLVLKQFLLLRDLNEVFTGGISSYSLILMCISFLQMHPRGIYHDTANLGVLLLEFFELYGRRFNYMKIGISIKNGGRYMPKDELQRDMVDGHRPSLLCIEDPLTPGNDIGRSSYGVFQVQQAFKCAYRVLALAVSPLNLLGIDPRVNSILGRIIHITDDVIDYREWIRENFEHLVVVDRISPLPTAAPTAYATANGAPKYVIMPSGAVVQQLYHHPVQVPTAHGHSHAHSHSHGHAHPGAHLCQPYVTGTTVSAVTTTTTMAVVTVGVSAGGVQQQQQQQNATAHTHSQQQTQNQSQSRHRRGSTSSGDDSEDSKDGDVVETTSSAQEVVDIALSTPNGLANMSMPMPVHAVGMPASNSWSGNGNGNGNSSSSTGSSPEIAHIAAQEMDPELEDQQQQQQHQETSGGNGFIRPGDVGTGSNRGGGDGSGGRNYNQRNNHNSSGYYHQQYYVPPPMQQQLSKSNSSSNYHQQHHHSHSHGNHSHRQQHHHQQQHHHQQRPQHLRVGGGNRYQKSLGGSPIISAGNASNSSSNCSNSSSSSGSNNSRLPPLRGTLVNSSSAISIISISSESSIASSSSSSSRSGQDQQRDER

Target ORF information:

RefSeq Version	NM_206658.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster topoisomerase related function 4-1, transcript variant E (Trf4-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206658.4

ORF Insert Sequence:

ATGGATCCAT TTGTTGGCTG GTTTCAGAAG GAACAGGAGG GACCATCGCT GTGCACATGG 
TTAAAGATTT GGGAGACGAA TGCCCAAGAG ATGGGCGCAT TGCTTAATCA ACAGCTACAG 
CAGGCGACGA CGATCAACGG GAGCACGAGC AGCAGCAGCA GCAGCAGCAA TAGTGGTAAC 
AACAACAACA ACAACAACAA TAACATCATC AATAACACTA TCACCAACAC GACCAATAAC 
ACTGGTAACA ATTCATCGGC CAAACCCTAC TTATCGCGAC CCTACAGCTC GCTAAATCGG 
GTGCTCAATT TCCGAGCGGA TTCCCTGGAG ATCCTGCAGC AGCAACAACA ACAGCAGCAG 
CTCAACGGGA CCACTCAGCG CAACAGCACC AACATCAATA CCACAAGCGG CGGCAGCACT 
AGCTCGTCGG CAGATTCCAC CACGAATCGG GACAATAACT CACCCGCCAA TAGCAGTTCA 
ACCAACGGAC CCGGAGCAGG AACGGGAACG TCAACAGGAG CAGGAGGTAC CGGTACAAAT 
AGTCCGGCGA CGACAGCGTC AAGCACAGCT GCGACCACGA CTGGTCCAGC CACTAGTATG 
AGCGACACCA GCAACAACCC GCCACAATCA ACGACCACGC CCGCCAGCCG CACGAACAGC 
ATCTACTACA ATCCATCGCG GAAGAAACGA CCGGAGAACA AGGCTGGCGG AGCCCACTAC 
TATATGAACA ACCATATGGA GATGATCGCC AAGTACAAGG GCGAACCGTG GCGCAAGCCG 
GACTATCCGT ATGGCGAGGG CGTTATCGGG CTGCACGAGG AGATCGAGCA TTTCTATCAG 
TACGTCCTGC CCACGCCCTG CGAACACGCC ATCCGCAACG AGGTGGTCAA GCGTATCGAG 
GCCGTTGTCC ATTCCATCTG GCCACAGGCA GTAGTCGAGA TCTTTGGCTC CTTTCGCACT 
GGCCTCTTTT TGCCCACCTC CGACATAGAT CTCGTTGTGT TAGGTTTGTG GGAGAAGCTG 
CCGCTGAGAA CGCTGGAGTT CGAGCTAGTG AGCCGTGGTA TCGCCGAGGC TTGTACGGTG 
CGTGTTCTGG ACAAGGCATC AGTGCCCATC ATCAAGCTGA CGGACCGGGA AACGCAAGTG 
AAGGTAGACA TATCGTTCAA CATGCAGAGT GGTGTTCAGT CGGCGGAGCT GATTAAGAAG 
TTTAAGCGCG ACTATCCAGT GCTGGAGAAG CTAGTGCTTG TGCTGAAGCA GTTCTTACTA 
CTTCGCGATC TCAACGAGGT GTTCACGGGC GGCATCTCCT CCTACTCGCT GATCCTTATG 
TGCATCAGCT TCCTGCAGAT GCATCCGCGC GGTATCTACC ACGATACAGC CAATCTGGGG 
GTGCTTCTCC TCGAGTTCTT CGAACTTTAT GGACGTCGCT TCAACTACAT GAAGATCGGC 
ATCTCCATAA AGAACGGTGG CCGCTACATG CCCAAGGATG AGCTGCAGCG TGACATGGTC 
GATGGCCATC GACCCTCGCT CCTGTGCATC GAGGATCCGC TGACGCCAGG CAATGATATC 
GGTCGAAGCA GCTACGGCGT CTTCCAGGTG CAACAGGCAT TCAAGTGCGC CTACCGCGTG 
CTCGCCCTTG CCGTCAGTCC GCTCAACCTT CTCGGCATCG ATCCGCGCGT CAACTCGATA 
CTGGGTCGAA TCATTCACAT CACCGACGAC GTGATCGATT ATCGCGAATG GATCCGCGAG 
AACTTCGAAC ATCTGGTGGT GGTGGATCGT ATCTCGCCGC TGCCCACTGC CGCTCCCACT 
GCGTACGCCA CCGCCAATGG GGCGCCAAAA TATGTGATTA TGCCCTCGGG CGCCGTTGTC 
CAGCAGCTGT ACCATCATCC GGTGCAGGTG CCCACCGCTC ACGGGCACAG CCATGCGCAC 
AGCCACAGTC ACGGGCACGC GCATCCTGGC GCCCATCTGT GCCAGCCGTA TGTGACCGGG 
ACCACTGTAT CTGCTGTAAC GACGACTACA ACGATGGCGG TGGTGACCGT GGGCGTGTCA 
GCCGGCGGAG TCCAACAGCA GCAACAGCAA CAGAATGCGA CGGCCCATAC CCACTCGCAA 
CAGCAGACAC AGAACCAATC GCAGTCGCGC CATAGACGCG GCAGCACCTC GTCGGGCGAT 
GACTCCGAGG ATAGCAAGGA CGGCGATGTT GTCGAGACGA CGTCGAGTGC TCAGGAGGTC 
GTTGACATCG CGCTATCGAC GCCAAATGGC CTGGCCAACA TGTCCATGCC CATGCCAGTG 
CATGCTGTCG GAATGCCTGC GTCGAACAGT TGGAGCGGTA ATGGCAATGG AAACGGAAAC 
AGCAGCTCAT CCACGGGCTC GTCGCCGGAA ATCGCACACA TCGCTGCCCA GGAAATGGAC 
CCCGAGCTTG AGGATCAACA GCAACAACAG CAGCACCAAG AGACTTCTGG AGGTAATGGC 
TTCATCCGAC CGGGTGACGT CGGCACCGGT TCCAACAGGG GAGGCGGTGA CGGCAGTGGC 
GGCCGCAATT ACAACCAGCG GAACAACCAC AACTCCAGCG GCTACTACCA CCAGCAGTAC 
TACGTGCCAC CGCCGATGCA ACAGCAGCTG AGCAAGTCCA ACTCCTCCTC CAACTATCAC 
CAGCAACACC ATCACAGCCA CAGCCATGGC AATCATAGTC ACCGACAACA GCATCACCAC 
CAGCAGCAGC ACCACCACCA GCAGCGGCCG CAGCATCTGC GTGTGGGCGG CGGCAATCGA 
TACCAGAAAT CGTTGGGCGG ATCGCCCATC ATCAGTGCTG GCAATGCGTC GAACAGCAGC 
AGTAATTGCA GCAACAGCAG CAGCAGCAGC GGCAGCAACA ACAGCCGACT GCCGCCGCTA 
AGGGGCACGC TGGTCAACTC ATCGTCAGCA ATATCAATCA TTTCCATATC CTCAGAATCA 
TCCATTGCTA GCAGCAGCTC CAGTTCGTCA AGATCCGGTC AGGATCAGCA GCGTGACGAG 
CGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trf4-1 ( NM_001272411.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259340.1 Drosophila melanogaster topoisomerase related function 4-1, transcript variant J (Trf4-1), mRNA.

Price & Availability↑

CloneID	OFa01133
Clone ID Related Accession (Same CDS sequence)	NM_206659.3 , NM_206658.4 , NM_001272411.1 , NM_132262.3 , NM_206660.2
Accession Version	NM_001272411.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3006bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	topoisomerase related function 4-1, isoform J
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCCAT TTGTTGGCTG GTTTCAGAAG GAACAGGAGG GACCATCGCT GTGCACATGG 
TTAAAGATTT GGGAGACGAA TGCCCAAGAG ATGGGCGCAT TGCTTAATCA ACAGCTACAG 
CAGGCGACGA CGATCAACGG GAGCACGAGC AGCAGCAGCA GCAGCAGCAA TAGTGGTAAC 
AACAACAACA ACAACAACAA TAACATCATC AATAACACTA TCACCAACAC GACCAATAAC 
ACTGGTAACA ATTCATCGGC CAAACCCTAC TTATCGCGAC CCTACAGCTC GCTAAATCGG 
GTGCTCAATT TCCGAGCGGA TTCCCTGGAG ATCCTGCAGC AGCAACAACA ACAGCAGCAG 
CTCAACGGGA CCACTCAGCG CAACAGCACC AACATCAATA CCACAAGCGG CGGCAGCACT 
AGCTCGTCGG CAGATTCCAC CACGAATCGG GACAATAACT CACCCGCCAA TAGCAGTTCA 
ACCAACGGAC CCGGAGCAGG AACGGGAACG TCAACAGGAG CAGGAGGTAC CGGTACAAAT 
AGTCCGGCGA CGACAGCGTC AAGCACAGCT GCGACCACGA CTGGTCCAGC CACTAGTATG 
AGCGACACCA GCAACAACCC GCCACAATCA ACGACCACGC CCGCCAGCCG CACGAACAGC 
ATCTACTACA ATCCATCGCG GAAGAAACGA CCGGAGAACA AGGCTGGCGG AGCCCACTAC 
TATATGAACA ACCATATGGA GATGATCGCC AAGTACAAGG GCGAACCGTG GCGCAAGCCG 
GACTATCCGT ATGGCGAGGG CGTTATCGGG CTGCACGAGG AGATCGAGCA TTTCTATCAG 
TACGTCCTGC CCACGCCCTG CGAACACGCC ATCCGCAACG AGGTGGTCAA GCGTATCGAG 
GCCGTTGTCC ATTCCATCTG GCCACAGGCA GTAGTCGAGA TCTTTGGCTC CTTTCGCACT 
GGCCTCTTTT TGCCCACCTC CGACATAGAT CTCGTTGTGT TAGGTTTGTG GGAGAAGCTG 
CCGCTGAGAA CGCTGGAGTT CGAGCTAGTG AGCCGTGGTA TCGCCGAGGC TTGTACGGTG 
CGTGTTCTGG ACAAGGCATC AGTGCCCATC ATCAAGCTGA CGGACCGGGA AACGCAAGTG 
AAGGTAGACA TATCGTTCAA CATGCAGAGT GGTGTTCAGT CGGCGGAGCT GATTAAGAAG 
TTTAAGCGCG ACTATCCAGT GCTGGAGAAG CTAGTGCTTG TGCTGAAGCA GTTCTTACTA 
CTTCGCGATC TCAACGAGGT GTTCACGGGC GGCATCTCCT CCTACTCGCT GATCCTTATG 
TGCATCAGCT TCCTGCAGAT GCATCCGCGC GGTATCTACC ACGATACAGC CAATCTGGGG 
GTGCTTCTCC TCGAGTTCTT CGAACTTTAT GGACGTCGCT TCAACTACAT GAAGATCGGC 
ATCTCCATAA AGAACGGTGG CCGCTACATG CCCAAGGATG AGCTGCAGCG TGACATGGTC 
GATGGCCATC GACCCTCGCT CCTGTGCATC GAGGATCCGC TGACGCCAGG CAATGATATC 
GGTCGAAGCA GCTACGGCGT CTTCCAGGTG CAACAGGCAT TCAAGTGCGC CTACCGCGTG 
CTCGCCCTTG CCGTCAGTCC GCTCAACCTT CTCGGCATCG ATCCGCGCGT CAACTCGATA 
CTGGGTCGAA TCATTCACAT CACCGACGAC GTGATCGATT ATCGCGAATG GATCCGCGAG 
AACTTCGAAC ATCTGGTGGT GGTGGATCGT ATCTCGCCGC TGCCCACTGC CGCTCCCACT 
GCGTACGCCA CCGCCAATGG GGCGCCAAAA TATGTGATTA TGCCCTCGGG CGCCGTTGTC 
CAGCAGCTGT ACCATCATCC GGTGCAGGTG CCCACCGCTC ACGGGCACAG CCATGCGCAC 
AGCCACAGTC ACGGGCACGC GCATCCTGGC GCCCATCTGT GCCAGCCGTA TGTGACCGGG 
ACCACTGTAT CTGCTGTAAC GACGACTACA ACGATGGCGG TGGTGACCGT GGGCGTGTCA 
GCCGGCGGAG TCCAACAGCA GCAACAGCAA CAGAATGCGA CGGCCCATAC CCACTCGCAA 
CAGCAGACAC AGAACCAATC GCAGTCGCGC CATAGACGCG GCAGCACCTC GTCGGGCGAT 
GACTCCGAGG ATAGCAAGGA CGGCGATGTT GTCGAGACGA CGTCGAGTGC TCAGGAGGTC 
GTTGACATCG CGCTATCGAC GCCAAATGGC CTGGCCAACA TGTCCATGCC CATGCCAGTG 
CATGCTGTCG GAATGCCTGC GTCGAACAGT TGGAGCGGTA ATGGCAATGG AAACGGAAAC 
AGCAGCTCAT CCACGGGCTC GTCGCCGGAA ATCGCACACA TCGCTGCCCA GGAAATGGAC 
CCCGAGCTTG AGGATCAACA GCAACAACAG CAGCACCAAG AGACTTCTGG AGGTAATGGC 
TTCATCCGAC CGGGTGACGT CGGCACCGGT TCCAACAGGG GAGGCGGTGA CGGCAGTGGC 
GGCCGCAATT ACAACCAGCG GAACAACCAC AACTCCAGCG GCTACTACCA CCAGCAGTAC 
TACGTGCCAC CGCCGATGCA ACAGCAGCTG AGCAAGTCCA ACTCCTCCTC CAACTATCAC 
CAGCAACACC ATCACAGCCA CAGCCATGGC AATCATAGTC ACCGACAACA GCATCACCAC 
CAGCAGCAGC ACCACCACCA GCAGCGGCCG CAGCATCTGC GTGTGGGCGG CGGCAATCGA 
TACCAGAAAT CGTTGGGCGG ATCGCCCATC ATCAGTGCTG GCAATGCGTC GAACAGCAGC 
AGTAATTGCA GCAACAGCAG CAGCAGCAGC GGCAGCAACA ACAGCCGACT GCCGCCGCTA 
AGGGGCACGC TGGTCAACTC ATCGTCAGCA ATATCAATCA TTTCCATATC CTCAGAATCA 
TCCATTGCTA GCAGCAGCTC CAGTTCGTCA AGATCCGGTC AGGATCAGCA GCGTGACGAG 
CGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259340.1
CDS	586..3591
Translation	MDPFVGWFQKEQEGPSLCTWLKIWETNAQEMGALLNQQLQQATTINGSTSSSSSSSNSGNNNNNNNNNIINNTITNTTNNTGNNSSAKPYLSRPYSSLNRVLNFRADSLEILQQQQQQQQLNGTTQRNSTNINTTSGGSTSSSADSTTNRDNNSPANSSSTNGPGAGTGTSTGAGGTGTNSPATTASSTAATTTGPATSMSDTSNNPPQSTTTPASRTNSIYYNPSRKKRPENKAGGAHYYMNNHMEMIAKYKGEPWRKPDYPYGEGVIGLHEEIEHFYQYVLPTPCEHAIRNEVVKRIEAVVHSIWPQAVVEIFGSFRTGLFLPTSDIDLVVLGLWEKLPLRTLEFELVSRGIAEACTVRVLDKASVPIIKLTDRETQVKVDISFNMQSGVQSAELIKKFKRDYPVLEKLVLVLKQFLLLRDLNEVFTGGISSYSLILMCISFLQMHPRGIYHDTANLGVLLLEFFELYGRRFNYMKIGISIKNGGRYMPKDELQRDMVDGHRPSLLCIEDPLTPGNDIGRSSYGVFQVQQAFKCAYRVLALAVSPLNLLGIDPRVNSILGRIIHITDDVIDYREWIRENFEHLVVVDRISPLPTAAPTAYATANGAPKYVIMPSGAVVQQLYHHPVQVPTAHGHSHAHSHSHGHAHPGAHLCQPYVTGTTVSAVTTTTTMAVVTVGVSAGGVQQQQQQQNATAHTHSQQQTQNQSQSRHRRGSTSSGDDSEDSKDGDVVETTSSAQEVVDIALSTPNGLANMSMPMPVHAVGMPASNSWSGNGNGNGNSSSSTGSSPEIAHIAAQEMDPELEDQQQQQQHQETSGGNGFIRPGDVGTGSNRGGGDGSGGRNYNQRNNHNSSGYYHQQYYVPPPMQQQLSKSNSSSNYHQQHHHSHSHGNHSHRQQHHHQQQHHHQQRPQHLRVGGGNRYQKSLGGSPIISAGNASNSSSNCSNSSSSSGSNNSRLPPLRGTLVNSSSAISIISISSESSIASSSSSSSRSGQDQQRDER

Target ORF information:

RefSeq Version	NM_001272411.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster topoisomerase related function 4-1, transcript variant J (Trf4-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272411.1

ORF Insert Sequence:

ATGGATCCAT TTGTTGGCTG GTTTCAGAAG GAACAGGAGG GACCATCGCT GTGCACATGG 
TTAAAGATTT GGGAGACGAA TGCCCAAGAG ATGGGCGCAT TGCTTAATCA ACAGCTACAG 
CAGGCGACGA CGATCAACGG GAGCACGAGC AGCAGCAGCA GCAGCAGCAA TAGTGGTAAC 
AACAACAACA ACAACAACAA TAACATCATC AATAACACTA TCACCAACAC GACCAATAAC 
ACTGGTAACA ATTCATCGGC CAAACCCTAC TTATCGCGAC CCTACAGCTC GCTAAATCGG 
GTGCTCAATT TCCGAGCGGA TTCCCTGGAG ATCCTGCAGC AGCAACAACA ACAGCAGCAG 
CTCAACGGGA CCACTCAGCG CAACAGCACC AACATCAATA CCACAAGCGG CGGCAGCACT 
AGCTCGTCGG CAGATTCCAC CACGAATCGG GACAATAACT CACCCGCCAA TAGCAGTTCA 
ACCAACGGAC CCGGAGCAGG AACGGGAACG TCAACAGGAG CAGGAGGTAC CGGTACAAAT 
AGTCCGGCGA CGACAGCGTC AAGCACAGCT GCGACCACGA CTGGTCCAGC CACTAGTATG 
AGCGACACCA GCAACAACCC GCCACAATCA ACGACCACGC CCGCCAGCCG CACGAACAGC 
ATCTACTACA ATCCATCGCG GAAGAAACGA CCGGAGAACA AGGCTGGCGG AGCCCACTAC 
TATATGAACA ACCATATGGA GATGATCGCC AAGTACAAGG GCGAACCGTG GCGCAAGCCG 
GACTATCCGT ATGGCGAGGG CGTTATCGGG CTGCACGAGG AGATCGAGCA TTTCTATCAG 
TACGTCCTGC CCACGCCCTG CGAACACGCC ATCCGCAACG AGGTGGTCAA GCGTATCGAG 
GCCGTTGTCC ATTCCATCTG GCCACAGGCA GTAGTCGAGA TCTTTGGCTC CTTTCGCACT 
GGCCTCTTTT TGCCCACCTC CGACATAGAT CTCGTTGTGT TAGGTTTGTG GGAGAAGCTG 
CCGCTGAGAA CGCTGGAGTT CGAGCTAGTG AGCCGTGGTA TCGCCGAGGC TTGTACGGTG 
CGTGTTCTGG ACAAGGCATC AGTGCCCATC ATCAAGCTGA CGGACCGGGA AACGCAAGTG 
AAGGTAGACA TATCGTTCAA CATGCAGAGT GGTGTTCAGT CGGCGGAGCT GATTAAGAAG 
TTTAAGCGCG ACTATCCAGT GCTGGAGAAG CTAGTGCTTG TGCTGAAGCA GTTCTTACTA 
CTTCGCGATC TCAACGAGGT GTTCACGGGC GGCATCTCCT CCTACTCGCT GATCCTTATG 
TGCATCAGCT TCCTGCAGAT GCATCCGCGC GGTATCTACC ACGATACAGC CAATCTGGGG 
GTGCTTCTCC TCGAGTTCTT CGAACTTTAT GGACGTCGCT TCAACTACAT GAAGATCGGC 
ATCTCCATAA AGAACGGTGG CCGCTACATG CCCAAGGATG AGCTGCAGCG TGACATGGTC 
GATGGCCATC GACCCTCGCT CCTGTGCATC GAGGATCCGC TGACGCCAGG CAATGATATC 
GGTCGAAGCA GCTACGGCGT CTTCCAGGTG CAACAGGCAT TCAAGTGCGC CTACCGCGTG 
CTCGCCCTTG CCGTCAGTCC GCTCAACCTT CTCGGCATCG ATCCGCGCGT CAACTCGATA 
CTGGGTCGAA TCATTCACAT CACCGACGAC GTGATCGATT ATCGCGAATG GATCCGCGAG 
AACTTCGAAC ATCTGGTGGT GGTGGATCGT ATCTCGCCGC TGCCCACTGC CGCTCCCACT 
GCGTACGCCA CCGCCAATGG GGCGCCAAAA TATGTGATTA TGCCCTCGGG CGCCGTTGTC 
CAGCAGCTGT ACCATCATCC GGTGCAGGTG CCCACCGCTC ACGGGCACAG CCATGCGCAC 
AGCCACAGTC ACGGGCACGC GCATCCTGGC GCCCATCTGT GCCAGCCGTA TGTGACCGGG 
ACCACTGTAT CTGCTGTAAC GACGACTACA ACGATGGCGG TGGTGACCGT GGGCGTGTCA 
GCCGGCGGAG TCCAACAGCA GCAACAGCAA CAGAATGCGA CGGCCCATAC CCACTCGCAA 
CAGCAGACAC AGAACCAATC GCAGTCGCGC CATAGACGCG GCAGCACCTC GTCGGGCGAT 
GACTCCGAGG ATAGCAAGGA CGGCGATGTT GTCGAGACGA CGTCGAGTGC TCAGGAGGTC 
GTTGACATCG CGCTATCGAC GCCAAATGGC CTGGCCAACA TGTCCATGCC CATGCCAGTG 
CATGCTGTCG GAATGCCTGC GTCGAACAGT TGGAGCGGTA ATGGCAATGG AAACGGAAAC 
AGCAGCTCAT CCACGGGCTC GTCGCCGGAA ATCGCACACA TCGCTGCCCA GGAAATGGAC 
CCCGAGCTTG AGGATCAACA GCAACAACAG CAGCACCAAG AGACTTCTGG AGGTAATGGC 
TTCATCCGAC CGGGTGACGT CGGCACCGGT TCCAACAGGG GAGGCGGTGA CGGCAGTGGC 
GGCCGCAATT ACAACCAGCG GAACAACCAC AACTCCAGCG GCTACTACCA CCAGCAGTAC 
TACGTGCCAC CGCCGATGCA ACAGCAGCTG AGCAAGTCCA ACTCCTCCTC CAACTATCAC 
CAGCAACACC ATCACAGCCA CAGCCATGGC AATCATAGTC ACCGACAACA GCATCACCAC 
CAGCAGCAGC ACCACCACCA GCAGCGGCCG CAGCATCTGC GTGTGGGCGG CGGCAATCGA 
TACCAGAAAT CGTTGGGCGG ATCGCCCATC ATCAGTGCTG GCAATGCGTC GAACAGCAGC 
AGTAATTGCA GCAACAGCAG CAGCAGCAGC GGCAGCAACA ACAGCCGACT GCCGCCGCTA 
AGGGGCACGC TGGTCAACTC ATCGTCAGCA ATATCAATCA TTTCCATATC CTCAGAATCA 
TCCATTGCTA GCAGCAGCTC CAGTTCGTCA AGATCCGGTC AGGATCAGCA GCGTGACGAG 
CGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trf4-1 ( NM_132262.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572490.2 Drosophila melanogaster topoisomerase related function 4-1, transcript variant I (Trf4-1), mRNA.

Price & Availability↑

CloneID	OFa01133
Clone ID Related Accession (Same CDS sequence)	NM_206659.3 , NM_206658.4 , NM_001272411.1 , NM_132262.3 , NM_206660.2
Accession Version	NM_132262.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3006bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	topoisomerase related function 4-1, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_132262.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCCAT TTGTTGGCTG GTTTCAGAAG GAACAGGAGG GACCATCGCT GTGCACATGG 
TTAAAGATTT GGGAGACGAA TGCCCAAGAG ATGGGCGCAT TGCTTAATCA ACAGCTACAG 
CAGGCGACGA CGATCAACGG GAGCACGAGC AGCAGCAGCA GCAGCAGCAA TAGTGGTAAC 
AACAACAACA ACAACAACAA TAACATCATC AATAACACTA TCACCAACAC GACCAATAAC 
ACTGGTAACA ATTCATCGGC CAAACCCTAC TTATCGCGAC CCTACAGCTC GCTAAATCGG 
GTGCTCAATT TCCGAGCGGA TTCCCTGGAG ATCCTGCAGC AGCAACAACA ACAGCAGCAG 
CTCAACGGGA CCACTCAGCG CAACAGCACC AACATCAATA CCACAAGCGG CGGCAGCACT 
AGCTCGTCGG CAGATTCCAC CACGAATCGG GACAATAACT CACCCGCCAA TAGCAGTTCA 
ACCAACGGAC CCGGAGCAGG AACGGGAACG TCAACAGGAG CAGGAGGTAC CGGTACAAAT 
AGTCCGGCGA CGACAGCGTC AAGCACAGCT GCGACCACGA CTGGTCCAGC CACTAGTATG 
AGCGACACCA GCAACAACCC GCCACAATCA ACGACCACGC CCGCCAGCCG CACGAACAGC 
ATCTACTACA ATCCATCGCG GAAGAAACGA CCGGAGAACA AGGCTGGCGG AGCCCACTAC 
TATATGAACA ACCATATGGA GATGATCGCC AAGTACAAGG GCGAACCGTG GCGCAAGCCG 
GACTATCCGT ATGGCGAGGG CGTTATCGGG CTGCACGAGG AGATCGAGCA TTTCTATCAG 
TACGTCCTGC CCACGCCCTG CGAACACGCC ATCCGCAACG AGGTGGTCAA GCGTATCGAG 
GCCGTTGTCC ATTCCATCTG GCCACAGGCA GTAGTCGAGA TCTTTGGCTC CTTTCGCACT 
GGCCTCTTTT TGCCCACCTC CGACATAGAT CTCGTTGTGT TAGGTTTGTG GGAGAAGCTG 
CCGCTGAGAA CGCTGGAGTT CGAGCTAGTG AGCCGTGGTA TCGCCGAGGC TTGTACGGTG 
CGTGTTCTGG ACAAGGCATC AGTGCCCATC ATCAAGCTGA CGGACCGGGA AACGCAAGTG 
AAGGTAGACA TATCGTTCAA CATGCAGAGT GGTGTTCAGT CGGCGGAGCT GATTAAGAAG 
TTTAAGCGCG ACTATCCAGT GCTGGAGAAG CTAGTGCTTG TGCTGAAGCA GTTCTTACTA 
CTTCGCGATC TCAACGAGGT GTTCACGGGC GGCATCTCCT CCTACTCGCT GATCCTTATG 
TGCATCAGCT TCCTGCAGAT GCATCCGCGC GGTATCTACC ACGATACAGC CAATCTGGGG 
GTGCTTCTCC TCGAGTTCTT CGAACTTTAT GGACGTCGCT TCAACTACAT GAAGATCGGC 
ATCTCCATAA AGAACGGTGG CCGCTACATG CCCAAGGATG AGCTGCAGCG TGACATGGTC 
GATGGCCATC GACCCTCGCT CCTGTGCATC GAGGATCCGC TGACGCCAGG CAATGATATC 
GGTCGAAGCA GCTACGGCGT CTTCCAGGTG CAACAGGCAT TCAAGTGCGC CTACCGCGTG 
CTCGCCCTTG CCGTCAGTCC GCTCAACCTT CTCGGCATCG ATCCGCGCGT CAACTCGATA 
CTGGGTCGAA TCATTCACAT CACCGACGAC GTGATCGATT ATCGCGAATG GATCCGCGAG 
AACTTCGAAC ATCTGGTGGT GGTGGATCGT ATCTCGCCGC TGCCCACTGC CGCTCCCACT 
GCGTACGCCA CCGCCAATGG GGCGCCAAAA TATGTGATTA TGCCCTCGGG CGCCGTTGTC 
CAGCAGCTGT ACCATCATCC GGTGCAGGTG CCCACCGCTC ACGGGCACAG CCATGCGCAC 
AGCCACAGTC ACGGGCACGC GCATCCTGGC GCCCATCTGT GCCAGCCGTA TGTGACCGGG 
ACCACTGTAT CTGCTGTAAC GACGACTACA ACGATGGCGG TGGTGACCGT GGGCGTGTCA 
GCCGGCGGAG TCCAACAGCA GCAACAGCAA CAGAATGCGA CGGCCCATAC CCACTCGCAA 
CAGCAGACAC AGAACCAATC GCAGTCGCGC CATAGACGCG GCAGCACCTC GTCGGGCGAT 
GACTCCGAGG ATAGCAAGGA CGGCGATGTT GTCGAGACGA CGTCGAGTGC TCAGGAGGTC 
GTTGACATCG CGCTATCGAC GCCAAATGGC CTGGCCAACA TGTCCATGCC CATGCCAGTG 
CATGCTGTCG GAATGCCTGC GTCGAACAGT TGGAGCGGTA ATGGCAATGG AAACGGAAAC 
AGCAGCTCAT CCACGGGCTC GTCGCCGGAA ATCGCACACA TCGCTGCCCA GGAAATGGAC 
CCCGAGCTTG AGGATCAACA GCAACAACAG CAGCACCAAG AGACTTCTGG AGGTAATGGC 
TTCATCCGAC CGGGTGACGT CGGCACCGGT TCCAACAGGG GAGGCGGTGA CGGCAGTGGC 
GGCCGCAATT ACAACCAGCG GAACAACCAC AACTCCAGCG GCTACTACCA CCAGCAGTAC 
TACGTGCCAC CGCCGATGCA ACAGCAGCTG AGCAAGTCCA ACTCCTCCTC CAACTATCAC 
CAGCAACACC ATCACAGCCA CAGCCATGGC AATCATAGTC ACCGACAACA GCATCACCAC 
CAGCAGCAGC ACCACCACCA GCAGCGGCCG CAGCATCTGC GTGTGGGCGG CGGCAATCGA 
TACCAGAAAT CGTTGGGCGG ATCGCCCATC ATCAGTGCTG GCAATGCGTC GAACAGCAGC 
AGTAATTGCA GCAACAGCAG CAGCAGCAGC GGCAGCAACA ACAGCCGACT GCCGCCGCTA 
AGGGGCACGC TGGTCAACTC ATCGTCAGCA ATATCAATCA TTTCCATATC CTCAGAATCA 
TCCATTGCTA GCAGCAGCTC CAGTTCGTCA AGATCCGGTC AGGATCAGCA GCGTGACGAG 
CGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572490.2
CDS	551..3556
Translation	MDPFVGWFQKEQEGPSLCTWLKIWETNAQEMGALLNQQLQQATTINGSTSSSSSSSNSGNNNNNNNNNIINNTITNTTNNTGNNSSAKPYLSRPYSSLNRVLNFRADSLEILQQQQQQQQLNGTTQRNSTNINTTSGGSTSSSADSTTNRDNNSPANSSSTNGPGAGTGTSTGAGGTGTNSPATTASSTAATTTGPATSMSDTSNNPPQSTTTPASRTNSIYYNPSRKKRPENKAGGAHYYMNNHMEMIAKYKGEPWRKPDYPYGEGVIGLHEEIEHFYQYVLPTPCEHAIRNEVVKRIEAVVHSIWPQAVVEIFGSFRTGLFLPTSDIDLVVLGLWEKLPLRTLEFELVSRGIAEACTVRVLDKASVPIIKLTDRETQVKVDISFNMQSGVQSAELIKKFKRDYPVLEKLVLVLKQFLLLRDLNEVFTGGISSYSLILMCISFLQMHPRGIYHDTANLGVLLLEFFELYGRRFNYMKIGISIKNGGRYMPKDELQRDMVDGHRPSLLCIEDPLTPGNDIGRSSYGVFQVQQAFKCAYRVLALAVSPLNLLGIDPRVNSILGRIIHITDDVIDYREWIRENFEHLVVVDRISPLPTAAPTAYATANGAPKYVIMPSGAVVQQLYHHPVQVPTAHGHSHAHSHSHGHAHPGAHLCQPYVTGTTVSAVTTTTTMAVVTVGVSAGGVQQQQQQQNATAHTHSQQQTQNQSQSRHRRGSTSSGDDSEDSKDGDVVETTSSAQEVVDIALSTPNGLANMSMPMPVHAVGMPASNSWSGNGNGNGNSSSSTGSSPEIAHIAAQEMDPELEDQQQQQQHQETSGGNGFIRPGDVGTGSNRGGGDGSGGRNYNQRNNHNSSGYYHQQYYVPPPMQQQLSKSNSSSNYHQQHHHSHSHGNHSHRQQHHHQQQHHHQQRPQHLRVGGGNRYQKSLGGSPIISAGNASNSSSNCSNSSSSSGSNNSRLPPLRGTLVNSSSAISIISISSESSIASSSSSSSRSGQDQQRDER

Target ORF information:

RefSeq Version	NM_132262.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster topoisomerase related function 4-1, transcript variant I (Trf4-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132262.3

ORF Insert Sequence:

ATGGATCCAT TTGTTGGCTG GTTTCAGAAG GAACAGGAGG GACCATCGCT GTGCACATGG 
TTAAAGATTT GGGAGACGAA TGCCCAAGAG ATGGGCGCAT TGCTTAATCA ACAGCTACAG 
CAGGCGACGA CGATCAACGG GAGCACGAGC AGCAGCAGCA GCAGCAGCAA TAGTGGTAAC 
AACAACAACA ACAACAACAA TAACATCATC AATAACACTA TCACCAACAC GACCAATAAC 
ACTGGTAACA ATTCATCGGC CAAACCCTAC TTATCGCGAC CCTACAGCTC GCTAAATCGG 
GTGCTCAATT TCCGAGCGGA TTCCCTGGAG ATCCTGCAGC AGCAACAACA ACAGCAGCAG 
CTCAACGGGA CCACTCAGCG CAACAGCACC AACATCAATA CCACAAGCGG CGGCAGCACT 
AGCTCGTCGG CAGATTCCAC CACGAATCGG GACAATAACT CACCCGCCAA TAGCAGTTCA 
ACCAACGGAC CCGGAGCAGG AACGGGAACG TCAACAGGAG CAGGAGGTAC CGGTACAAAT 
AGTCCGGCGA CGACAGCGTC AAGCACAGCT GCGACCACGA CTGGTCCAGC CACTAGTATG 
AGCGACACCA GCAACAACCC GCCACAATCA ACGACCACGC CCGCCAGCCG CACGAACAGC 
ATCTACTACA ATCCATCGCG GAAGAAACGA CCGGAGAACA AGGCTGGCGG AGCCCACTAC 
TATATGAACA ACCATATGGA GATGATCGCC AAGTACAAGG GCGAACCGTG GCGCAAGCCG 
GACTATCCGT ATGGCGAGGG CGTTATCGGG CTGCACGAGG AGATCGAGCA TTTCTATCAG 
TACGTCCTGC CCACGCCCTG CGAACACGCC ATCCGCAACG AGGTGGTCAA GCGTATCGAG 
GCCGTTGTCC ATTCCATCTG GCCACAGGCA GTAGTCGAGA TCTTTGGCTC CTTTCGCACT 
GGCCTCTTTT TGCCCACCTC CGACATAGAT CTCGTTGTGT TAGGTTTGTG GGAGAAGCTG 
CCGCTGAGAA CGCTGGAGTT CGAGCTAGTG AGCCGTGGTA TCGCCGAGGC TTGTACGGTG 
CGTGTTCTGG ACAAGGCATC AGTGCCCATC ATCAAGCTGA CGGACCGGGA AACGCAAGTG 
AAGGTAGACA TATCGTTCAA CATGCAGAGT GGTGTTCAGT CGGCGGAGCT GATTAAGAAG 
TTTAAGCGCG ACTATCCAGT GCTGGAGAAG CTAGTGCTTG TGCTGAAGCA GTTCTTACTA 
CTTCGCGATC TCAACGAGGT GTTCACGGGC GGCATCTCCT CCTACTCGCT GATCCTTATG 
TGCATCAGCT TCCTGCAGAT GCATCCGCGC GGTATCTACC ACGATACAGC CAATCTGGGG 
GTGCTTCTCC TCGAGTTCTT CGAACTTTAT GGACGTCGCT TCAACTACAT GAAGATCGGC 
ATCTCCATAA AGAACGGTGG CCGCTACATG CCCAAGGATG AGCTGCAGCG TGACATGGTC 
GATGGCCATC GACCCTCGCT CCTGTGCATC GAGGATCCGC TGACGCCAGG CAATGATATC 
GGTCGAAGCA GCTACGGCGT CTTCCAGGTG CAACAGGCAT TCAAGTGCGC CTACCGCGTG 
CTCGCCCTTG CCGTCAGTCC GCTCAACCTT CTCGGCATCG ATCCGCGCGT CAACTCGATA 
CTGGGTCGAA TCATTCACAT CACCGACGAC GTGATCGATT ATCGCGAATG GATCCGCGAG 
AACTTCGAAC ATCTGGTGGT GGTGGATCGT ATCTCGCCGC TGCCCACTGC CGCTCCCACT 
GCGTACGCCA CCGCCAATGG GGCGCCAAAA TATGTGATTA TGCCCTCGGG CGCCGTTGTC 
CAGCAGCTGT ACCATCATCC GGTGCAGGTG CCCACCGCTC ACGGGCACAG CCATGCGCAC 
AGCCACAGTC ACGGGCACGC GCATCCTGGC GCCCATCTGT GCCAGCCGTA TGTGACCGGG 
ACCACTGTAT CTGCTGTAAC GACGACTACA ACGATGGCGG TGGTGACCGT GGGCGTGTCA 
GCCGGCGGAG TCCAACAGCA GCAACAGCAA CAGAATGCGA CGGCCCATAC CCACTCGCAA 
CAGCAGACAC AGAACCAATC GCAGTCGCGC CATAGACGCG GCAGCACCTC GTCGGGCGAT 
GACTCCGAGG ATAGCAAGGA CGGCGATGTT GTCGAGACGA CGTCGAGTGC TCAGGAGGTC 
GTTGACATCG CGCTATCGAC GCCAAATGGC CTGGCCAACA TGTCCATGCC CATGCCAGTG 
CATGCTGTCG GAATGCCTGC GTCGAACAGT TGGAGCGGTA ATGGCAATGG AAACGGAAAC 
AGCAGCTCAT CCACGGGCTC GTCGCCGGAA ATCGCACACA TCGCTGCCCA GGAAATGGAC 
CCCGAGCTTG AGGATCAACA GCAACAACAG CAGCACCAAG AGACTTCTGG AGGTAATGGC 
TTCATCCGAC CGGGTGACGT CGGCACCGGT TCCAACAGGG GAGGCGGTGA CGGCAGTGGC 
GGCCGCAATT ACAACCAGCG GAACAACCAC AACTCCAGCG GCTACTACCA CCAGCAGTAC 
TACGTGCCAC CGCCGATGCA ACAGCAGCTG AGCAAGTCCA ACTCCTCCTC CAACTATCAC 
CAGCAACACC ATCACAGCCA CAGCCATGGC AATCATAGTC ACCGACAACA GCATCACCAC 
CAGCAGCAGC ACCACCACCA GCAGCGGCCG CAGCATCTGC GTGTGGGCGG CGGCAATCGA 
TACCAGAAAT CGTTGGGCGG ATCGCCCATC ATCAGTGCTG GCAATGCGTC GAACAGCAGC 
AGTAATTGCA GCAACAGCAG CAGCAGCAGC GGCAGCAACA ACAGCCGACT GCCGCCGCTA 
AGGGGCACGC TGGTCAACTC ATCGTCAGCA ATATCAATCA TTTCCATATC CTCAGAATCA 
TCCATTGCTA GCAGCAGCTC CAGTTCGTCA AGATCCGGTC AGGATCAGCA GCGTGACGAG 
CGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trf4-1 ( NM_206660.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996383.2 Drosophila melanogaster topoisomerase related function 4-1, transcript variant H (Trf4-1), mRNA.

Price & Availability↑

CloneID	OFa01133
Clone ID Related Accession (Same CDS sequence)	NM_206659.3 , NM_206658.4 , NM_001272411.1 , NM_132262.3 , NM_206660.2
Accession Version	NM_206660.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3006bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	topoisomerase related function 4-1, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_206660.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATCCAT TTGTTGGCTG GTTTCAGAAG GAACAGGAGG GACCATCGCT GTGCACATGG 
TTAAAGATTT GGGAGACGAA TGCCCAAGAG ATGGGCGCAT TGCTTAATCA ACAGCTACAG 
CAGGCGACGA CGATCAACGG GAGCACGAGC AGCAGCAGCA GCAGCAGCAA TAGTGGTAAC 
AACAACAACA ACAACAACAA TAACATCATC AATAACACTA TCACCAACAC GACCAATAAC 
ACTGGTAACA ATTCATCGGC CAAACCCTAC TTATCGCGAC CCTACAGCTC GCTAAATCGG 
GTGCTCAATT TCCGAGCGGA TTCCCTGGAG ATCCTGCAGC AGCAACAACA ACAGCAGCAG 
CTCAACGGGA CCACTCAGCG CAACAGCACC AACATCAATA CCACAAGCGG CGGCAGCACT 
AGCTCGTCGG CAGATTCCAC CACGAATCGG GACAATAACT CACCCGCCAA TAGCAGTTCA 
ACCAACGGAC CCGGAGCAGG AACGGGAACG TCAACAGGAG CAGGAGGTAC CGGTACAAAT 
AGTCCGGCGA CGACAGCGTC AAGCACAGCT GCGACCACGA CTGGTCCAGC CACTAGTATG 
AGCGACACCA GCAACAACCC GCCACAATCA ACGACCACGC CCGCCAGCCG CACGAACAGC 
ATCTACTACA ATCCATCGCG GAAGAAACGA CCGGAGAACA AGGCTGGCGG AGCCCACTAC 
TATATGAACA ACCATATGGA GATGATCGCC AAGTACAAGG GCGAACCGTG GCGCAAGCCG 
GACTATCCGT ATGGCGAGGG CGTTATCGGG CTGCACGAGG AGATCGAGCA TTTCTATCAG 
TACGTCCTGC CCACGCCCTG CGAACACGCC ATCCGCAACG AGGTGGTCAA GCGTATCGAG 
GCCGTTGTCC ATTCCATCTG GCCACAGGCA GTAGTCGAGA TCTTTGGCTC CTTTCGCACT 
GGCCTCTTTT TGCCCACCTC CGACATAGAT CTCGTTGTGT TAGGTTTGTG GGAGAAGCTG 
CCGCTGAGAA CGCTGGAGTT CGAGCTAGTG AGCCGTGGTA TCGCCGAGGC TTGTACGGTG 
CGTGTTCTGG ACAAGGCATC AGTGCCCATC ATCAAGCTGA CGGACCGGGA AACGCAAGTG 
AAGGTAGACA TATCGTTCAA CATGCAGAGT GGTGTTCAGT CGGCGGAGCT GATTAAGAAG 
TTTAAGCGCG ACTATCCAGT GCTGGAGAAG CTAGTGCTTG TGCTGAAGCA GTTCTTACTA 
CTTCGCGATC TCAACGAGGT GTTCACGGGC GGCATCTCCT CCTACTCGCT GATCCTTATG 
TGCATCAGCT TCCTGCAGAT GCATCCGCGC GGTATCTACC ACGATACAGC CAATCTGGGG 
GTGCTTCTCC TCGAGTTCTT CGAACTTTAT GGACGTCGCT TCAACTACAT GAAGATCGGC 
ATCTCCATAA AGAACGGTGG CCGCTACATG CCCAAGGATG AGCTGCAGCG TGACATGGTC 
GATGGCCATC GACCCTCGCT CCTGTGCATC GAGGATCCGC TGACGCCAGG CAATGATATC 
GGTCGAAGCA GCTACGGCGT CTTCCAGGTG CAACAGGCAT TCAAGTGCGC CTACCGCGTG 
CTCGCCCTTG CCGTCAGTCC GCTCAACCTT CTCGGCATCG ATCCGCGCGT CAACTCGATA 
CTGGGTCGAA TCATTCACAT CACCGACGAC GTGATCGATT ATCGCGAATG GATCCGCGAG 
AACTTCGAAC ATCTGGTGGT GGTGGATCGT ATCTCGCCGC TGCCCACTGC CGCTCCCACT 
GCGTACGCCA CCGCCAATGG GGCGCCAAAA TATGTGATTA TGCCCTCGGG CGCCGTTGTC 
CAGCAGCTGT ACCATCATCC GGTGCAGGTG CCCACCGCTC ACGGGCACAG CCATGCGCAC 
AGCCACAGTC ACGGGCACGC GCATCCTGGC GCCCATCTGT GCCAGCCGTA TGTGACCGGG 
ACCACTGTAT CTGCTGTAAC GACGACTACA ACGATGGCGG TGGTGACCGT GGGCGTGTCA 
GCCGGCGGAG TCCAACAGCA GCAACAGCAA CAGAATGCGA CGGCCCATAC CCACTCGCAA 
CAGCAGACAC AGAACCAATC GCAGTCGCGC CATAGACGCG GCAGCACCTC GTCGGGCGAT 
GACTCCGAGG ATAGCAAGGA CGGCGATGTT GTCGAGACGA CGTCGAGTGC TCAGGAGGTC 
GTTGACATCG CGCTATCGAC GCCAAATGGC CTGGCCAACA TGTCCATGCC CATGCCAGTG 
CATGCTGTCG GAATGCCTGC GTCGAACAGT TGGAGCGGTA ATGGCAATGG AAACGGAAAC 
AGCAGCTCAT CCACGGGCTC GTCGCCGGAA ATCGCACACA TCGCTGCCCA GGAAATGGAC 
CCCGAGCTTG AGGATCAACA GCAACAACAG CAGCACCAAG AGACTTCTGG AGGTAATGGC 
TTCATCCGAC CGGGTGACGT CGGCACCGGT TCCAACAGGG GAGGCGGTGA CGGCAGTGGC 
GGCCGCAATT ACAACCAGCG GAACAACCAC AACTCCAGCG GCTACTACCA CCAGCAGTAC 
TACGTGCCAC CGCCGATGCA ACAGCAGCTG AGCAAGTCCA ACTCCTCCTC CAACTATCAC 
CAGCAACACC ATCACAGCCA CAGCCATGGC AATCATAGTC ACCGACAACA GCATCACCAC 
CAGCAGCAGC ACCACCACCA GCAGCGGCCG CAGCATCTGC GTGTGGGCGG CGGCAATCGA 
TACCAGAAAT CGTTGGGCGG ATCGCCCATC ATCAGTGCTG GCAATGCGTC GAACAGCAGC 
AGTAATTGCA GCAACAGCAG CAGCAGCAGC GGCAGCAACA ACAGCCGACT GCCGCCGCTA 
AGGGGCACGC TGGTCAACTC ATCGTCAGCA ATATCAATCA TTTCCATATC CTCAGAATCA 
TCCATTGCTA GCAGCAGCTC CAGTTCGTCA AGATCCGGTC AGGATCAGCA GCGTGACGAG 
CGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996383.2
CDS	288..3293
Translation	MDPFVGWFQKEQEGPSLCTWLKIWETNAQEMGALLNQQLQQATTINGSTSSSSSSSNSGNNNNNNNNNIINNTITNTTNNTGNNSSAKPYLSRPYSSLNRVLNFRADSLEILQQQQQQQQLNGTTQRNSTNINTTSGGSTSSSADSTTNRDNNSPANSSSTNGPGAGTGTSTGAGGTGTNSPATTASSTAATTTGPATSMSDTSNNPPQSTTTPASRTNSIYYNPSRKKRPENKAGGAHYYMNNHMEMIAKYKGEPWRKPDYPYGEGVIGLHEEIEHFYQYVLPTPCEHAIRNEVVKRIEAVVHSIWPQAVVEIFGSFRTGLFLPTSDIDLVVLGLWEKLPLRTLEFELVSRGIAEACTVRVLDKASVPIIKLTDRETQVKVDISFNMQSGVQSAELIKKFKRDYPVLEKLVLVLKQFLLLRDLNEVFTGGISSYSLILMCISFLQMHPRGIYHDTANLGVLLLEFFELYGRRFNYMKIGISIKNGGRYMPKDELQRDMVDGHRPSLLCIEDPLTPGNDIGRSSYGVFQVQQAFKCAYRVLALAVSPLNLLGIDPRVNSILGRIIHITDDVIDYREWIRENFEHLVVVDRISPLPTAAPTAYATANGAPKYVIMPSGAVVQQLYHHPVQVPTAHGHSHAHSHSHGHAHPGAHLCQPYVTGTTVSAVTTTTTMAVVTVGVSAGGVQQQQQQQNATAHTHSQQQTQNQSQSRHRRGSTSSGDDSEDSKDGDVVETTSSAQEVVDIALSTPNGLANMSMPMPVHAVGMPASNSWSGNGNGNGNSSSSTGSSPEIAHIAAQEMDPELEDQQQQQQHQETSGGNGFIRPGDVGTGSNRGGGDGSGGRNYNQRNNHNSSGYYHQQYYVPPPMQQQLSKSNSSSNYHQQHHHSHSHGNHSHRQQHHHQQQHHHQQRPQHLRVGGGNRYQKSLGGSPIISAGNASNSSSNCSNSSSSSGSNNSRLPPLRGTLVNSSSAISIISISSESSIASSSSSSSRSGQDQQRDER

Target ORF information:

RefSeq Version	NM_206660.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster topoisomerase related function 4-1, transcript variant H (Trf4-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206660.2

ORF Insert Sequence:

ATGGATCCAT TTGTTGGCTG GTTTCAGAAG GAACAGGAGG GACCATCGCT GTGCACATGG 
TTAAAGATTT GGGAGACGAA TGCCCAAGAG ATGGGCGCAT TGCTTAATCA ACAGCTACAG 
CAGGCGACGA CGATCAACGG GAGCACGAGC AGCAGCAGCA GCAGCAGCAA TAGTGGTAAC 
AACAACAACA ACAACAACAA TAACATCATC AATAACACTA TCACCAACAC GACCAATAAC 
ACTGGTAACA ATTCATCGGC CAAACCCTAC TTATCGCGAC CCTACAGCTC GCTAAATCGG 
GTGCTCAATT TCCGAGCGGA TTCCCTGGAG ATCCTGCAGC AGCAACAACA ACAGCAGCAG 
CTCAACGGGA CCACTCAGCG CAACAGCACC AACATCAATA CCACAAGCGG CGGCAGCACT 
AGCTCGTCGG CAGATTCCAC CACGAATCGG GACAATAACT CACCCGCCAA TAGCAGTTCA 
ACCAACGGAC CCGGAGCAGG AACGGGAACG TCAACAGGAG CAGGAGGTAC CGGTACAAAT 
AGTCCGGCGA CGACAGCGTC AAGCACAGCT GCGACCACGA CTGGTCCAGC CACTAGTATG 
AGCGACACCA GCAACAACCC GCCACAATCA ACGACCACGC CCGCCAGCCG CACGAACAGC 
ATCTACTACA ATCCATCGCG GAAGAAACGA CCGGAGAACA AGGCTGGCGG AGCCCACTAC 
TATATGAACA ACCATATGGA GATGATCGCC AAGTACAAGG GCGAACCGTG GCGCAAGCCG 
GACTATCCGT ATGGCGAGGG CGTTATCGGG CTGCACGAGG AGATCGAGCA TTTCTATCAG 
TACGTCCTGC CCACGCCCTG CGAACACGCC ATCCGCAACG AGGTGGTCAA GCGTATCGAG 
GCCGTTGTCC ATTCCATCTG GCCACAGGCA GTAGTCGAGA TCTTTGGCTC CTTTCGCACT 
GGCCTCTTTT TGCCCACCTC CGACATAGAT CTCGTTGTGT TAGGTTTGTG GGAGAAGCTG 
CCGCTGAGAA CGCTGGAGTT CGAGCTAGTG AGCCGTGGTA TCGCCGAGGC TTGTACGGTG 
CGTGTTCTGG ACAAGGCATC AGTGCCCATC ATCAAGCTGA CGGACCGGGA AACGCAAGTG 
AAGGTAGACA TATCGTTCAA CATGCAGAGT GGTGTTCAGT CGGCGGAGCT GATTAAGAAG 
TTTAAGCGCG ACTATCCAGT GCTGGAGAAG CTAGTGCTTG TGCTGAAGCA GTTCTTACTA 
CTTCGCGATC TCAACGAGGT GTTCACGGGC GGCATCTCCT CCTACTCGCT GATCCTTATG 
TGCATCAGCT TCCTGCAGAT GCATCCGCGC GGTATCTACC ACGATACAGC CAATCTGGGG 
GTGCTTCTCC TCGAGTTCTT CGAACTTTAT GGACGTCGCT TCAACTACAT GAAGATCGGC 
ATCTCCATAA AGAACGGTGG CCGCTACATG CCCAAGGATG AGCTGCAGCG TGACATGGTC 
GATGGCCATC GACCCTCGCT CCTGTGCATC GAGGATCCGC TGACGCCAGG CAATGATATC 
GGTCGAAGCA GCTACGGCGT CTTCCAGGTG CAACAGGCAT TCAAGTGCGC CTACCGCGTG 
CTCGCCCTTG CCGTCAGTCC GCTCAACCTT CTCGGCATCG ATCCGCGCGT CAACTCGATA 
CTGGGTCGAA TCATTCACAT CACCGACGAC GTGATCGATT ATCGCGAATG GATCCGCGAG 
AACTTCGAAC ATCTGGTGGT GGTGGATCGT ATCTCGCCGC TGCCCACTGC CGCTCCCACT 
GCGTACGCCA CCGCCAATGG GGCGCCAAAA TATGTGATTA TGCCCTCGGG CGCCGTTGTC 
CAGCAGCTGT ACCATCATCC GGTGCAGGTG CCCACCGCTC ACGGGCACAG CCATGCGCAC 
AGCCACAGTC ACGGGCACGC GCATCCTGGC GCCCATCTGT GCCAGCCGTA TGTGACCGGG 
ACCACTGTAT CTGCTGTAAC GACGACTACA ACGATGGCGG TGGTGACCGT GGGCGTGTCA 
GCCGGCGGAG TCCAACAGCA GCAACAGCAA CAGAATGCGA CGGCCCATAC CCACTCGCAA 
CAGCAGACAC AGAACCAATC GCAGTCGCGC CATAGACGCG GCAGCACCTC GTCGGGCGAT 
GACTCCGAGG ATAGCAAGGA CGGCGATGTT GTCGAGACGA CGTCGAGTGC TCAGGAGGTC 
GTTGACATCG CGCTATCGAC GCCAAATGGC CTGGCCAACA TGTCCATGCC CATGCCAGTG 
CATGCTGTCG GAATGCCTGC GTCGAACAGT TGGAGCGGTA ATGGCAATGG AAACGGAAAC 
AGCAGCTCAT CCACGGGCTC GTCGCCGGAA ATCGCACACA TCGCTGCCCA GGAAATGGAC 
CCCGAGCTTG AGGATCAACA GCAACAACAG CAGCACCAAG AGACTTCTGG AGGTAATGGC 
TTCATCCGAC CGGGTGACGT CGGCACCGGT TCCAACAGGG GAGGCGGTGA CGGCAGTGGC 
GGCCGCAATT ACAACCAGCG GAACAACCAC AACTCCAGCG GCTACTACCA CCAGCAGTAC 
TACGTGCCAC CGCCGATGCA ACAGCAGCTG AGCAAGTCCA ACTCCTCCTC CAACTATCAC 
CAGCAACACC ATCACAGCCA CAGCCATGGC AATCATAGTC ACCGACAACA GCATCACCAC 
CAGCAGCAGC ACCACCACCA GCAGCGGCCG CAGCATCTGC GTGTGGGCGG CGGCAATCGA 
TACCAGAAAT CGTTGGGCGG ATCGCCCATC ATCAGTGCTG GCAATGCGTC GAACAGCAGC 
AGTAATTGCA GCAACAGCAG CAGCAGCAGC GGCAGCAACA ACAGCCGACT GCCGCCGCTA 
AGGGGCACGC TGGTCAACTC ATCGTCAGCA ATATCAATCA TTTCCATATC CTCAGAATCA 
TCCATTGCTA GCAGCAGCTC CAGTTCGTCA AGATCCGGTC AGGATCAGCA GCGTGACGAG 
CGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trf4-1 ( NM_001103443.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001096913.1 Drosophila melanogaster topoisomerase related function 4-1, transcript variant F (Trf4-1), mRNA.

Price & Availability↑

CloneID	OFa01134
Clone ID Related Accession (Same CDS sequence)	NM_001103443.2 , NM_001103444.2
Accession Version	NM_001103443.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	topoisomerase related function 4-1, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_001103443.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGACA CCAGCAACAA CCCGCCACAA TCAACGACCA CGCCCGCCAG CCGCACGAAC 
AGCATCTACT ACAATCCATC GCGGAAGAAA CGACCGGAGA ACAAGGCTGG CGGAGCCCAC 
TACTATATGA ACAACCATAT GGAGATGATC GCCAAGTACA AGGGCGAACC GTGGCGCAAG 
CCGGACTATC CGTATGGCGA GGGCGTTATC GGGCTGCACG AGGAGATCGA GCATTTCTAT 
CAGTACGTCC TGCCCACGCC CTGCGAACAC GCCATCCGCA ACGAGGTGGT CAAGCGTATC 
GAGGCCGTTG TCCATTCCAT CTGGCCACAG GCAGTAGTCG AGATCTTTGG CTCCTTTCGC 
ACTGGCCTCT TTTTGCCCAC CTCCGACATA GATCTCGTTG TGTTAGGTTT GTGGGAGAAG 
CTGCCGCTGA GAACGCTGGA GTTCGAGCTA GTGAGCCGTG GTATCGCCGA GGCTTGTACG 
GTGCGTGTTC TGGACAAGGC ATCAGTGCCC ATCATCAAGC TGACGGACCG GGAAACGCAA 
GTGAAGGTAG ACATATCGTT CAACATGCAG AGTGGTGTTC AGTCGGCGGA GCTGATTAAG 
AAGTTTAAGC GCGACTATCC AGTGCTGGAG AAGCTAGTGC TTGTGCTGAA GCAGTTCTTA 
CTACTTCGCG ATCTCAACGA GGTGTTCACG GGCGGCATCT CCTCCTACTC GCTGATCCTT 
ATGTGCATCA GCTTCCTGCA GATGCATCCG CGCGGTATCT ACCACGATAC AGCCAATCTG 
GGGGTGCTTC TCCTCGAGTT CTTCGAACTT TATGGACGTC GCTTCAACTA CATGAAGATC 
GGCATCTCCA TAAAGAACGG TGGCCGCTAC ATGCCCAAGG ATGAGCTGCA GCGTGACATG 
GTCGATGGCC ATCGACCCTC GCTCCTGTGC ATCGAGGATC CGCTGACGCC AGGCAATGAT 
ATCGGTCGAA GCAGCTACGG CGTCTTCCAG GTGCAACAGG CATTCAAGTG CGCCTACCGC 
GTGCTCGCCC TTGCCGTCAG TCCGCTCAAC CTTCTCGGCA TCGATCCGCG CGTCAACTCG 
ATACTGGGTC GAATCATTCA CATCACCGAC GACGTGATCG ATTATCGCGA ATGGATCCGC 
GAGAACTTCG AACATCTGGT GGTGGTGGAT CGTATCTCGC CGCTGCCCAC TGCCGCTCCC 
ACTGCGTACG CCACCGCCAA TGGGGCGCCA AAATATGTGA TTATGCCCTC GGGCGCCGTT 
GTCCAGCAGC TGTACCATCA TCCGGTGCAG GTGCCCACCG CTCACGGGCA CAGCCATGCG 
CACAGCCACA GTCACGGGCA CGCGCATCCT GGCGCCCATC TGTGCCAGCC GTATGTGACC 
GGGACCACTG TATCTGCTGT AACGACGACT ACAACGATGG CGGTGGTGAC CGTGGGCGTG 
TCAGCCGGCG GAGTCCAACA GCAGCAACAG CAACAGAATG CGACGGCCCA TACCCACTCG 
CAACAGCAGA CACAGAACCA ATCGCAGTCG CGCCATAGAC GCGGCAGCAC CTCGTCGGGC 
GATGACTCCG AGGATAGCAA GGACGGCGAT GTTGTCGAGA CGACGTCGAG TGCTCAGGAG 
GTCGTTGACA TCGCGCTATC GACGCCAAAT GGCCTGGCCA ACATGTCCAT GCCCATGCCA 
GTGCATGCTG TCGGAATGCC TGCGTCGAAC AGTTGGAGCG GTAATGGCAA TGGAAACGGA 
AACAGCAGCT CATCCACGGG CTCGTCGCCG GAAATCGCAC ACATCGCTGC CCAGGAAATG 
GACCCCGAGC TTGAGGATCA ACAGCAACAA CAGCAGCACC AAGAGACTTC TGGAGGTAAT 
GGCTTCATCC GACCGGGTGA CGTCGGCACC GGTTCCAACA GGGGAGGCGG TGACGGCAGT 
GGCGGCCGCA ATTACAACCA GCGGAACAAC CACAACTCCA GCGGCTACTA CCACCAGCAG 
TACTACGTGC CACCGCCGAT GCAACAGCAG CTGAGCAAGT CCAACTCCTC CTCCAACTAT 
CACCAGCAAC ACCATCACAG CCACAGCCAT GGCAATCATA GTCACCGACA ACAGCATCAC 
CACCAGCAGC AGCACCACCA CCAGCAGCGG CCGCAGCATC TGCGTGTGGG CGGCGGCAAT 
CGATACCAGA AATCGTTGGG CGGATCGCCC ATCATCAGTG CTGGCAATGC GTCGAACAGC 
AGCAGTAATT GCAGCAACAG CAGCAGCAGC AGCGGCAGCA ACAACAGCCG ACTGCCGCCG 
CTAAGGGGCA CGCTGGTCAA CTCATCGTCA GCAATATCAA TCATTTCCAT ATCCTCAGAA 
TCATCCATTG CTAGCAGCAG CTCCAGTTCG TCAAGATCCG GTCAGGATCA GCAGCGTGAC 
GAGCGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001096913.1
CDS	891..3299
Translation	MSDTSNNPPQSTTTPASRTNSIYYNPSRKKRPENKAGGAHYYMNNHMEMIAKYKGEPWRKPDYPYGEGVIGLHEEIEHFYQYVLPTPCEHAIRNEVVKRIEAVVHSIWPQAVVEIFGSFRTGLFLPTSDIDLVVLGLWEKLPLRTLEFELVSRGIAEACTVRVLDKASVPIIKLTDRETQVKVDISFNMQSGVQSAELIKKFKRDYPVLEKLVLVLKQFLLLRDLNEVFTGGISSYSLILMCISFLQMHPRGIYHDTANLGVLLLEFFELYGRRFNYMKIGISIKNGGRYMPKDELQRDMVDGHRPSLLCIEDPLTPGNDIGRSSYGVFQVQQAFKCAYRVLALAVSPLNLLGIDPRVNSILGRIIHITDDVIDYREWIRENFEHLVVVDRISPLPTAAPTAYATANGAPKYVIMPSGAVVQQLYHHPVQVPTAHGHSHAHSHSHGHAHPGAHLCQPYVTGTTVSAVTTTTTMAVVTVGVSAGGVQQQQQQQNATAHTHSQQQTQNQSQSRHRRGSTSSGDDSEDSKDGDVVETTSSAQEVVDIALSTPNGLANMSMPMPVHAVGMPASNSWSGNGNGNGNSSSSTGSSPEIAHIAAQEMDPELEDQQQQQQHQETSGGNGFIRPGDVGTGSNRGGGDGSGGRNYNQRNNHNSSGYYHQQYYVPPPMQQQLSKSNSSSNYHQQHHHSHSHGNHSHRQQHHHQQQHHHQQRPQHLRVGGGNRYQKSLGGSPIISAGNASNSSSNCSNSSSSSGSNNSRLPPLRGTLVNSSSAISIISISSESSIASSSSSSSRSGQDQQRDER

Target ORF information:

RefSeq Version	NM_001103443.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster topoisomerase related function 4-1, transcript variant F (Trf4-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103443.2

ORF Insert Sequence:

ATGAGCGACA CCAGCAACAA CCCGCCACAA TCAACGACCA CGCCCGCCAG CCGCACGAAC 
AGCATCTACT ACAATCCATC GCGGAAGAAA CGACCGGAGA ACAAGGCTGG CGGAGCCCAC 
TACTATATGA ACAACCATAT GGAGATGATC GCCAAGTACA AGGGCGAACC GTGGCGCAAG 
CCGGACTATC CGTATGGCGA GGGCGTTATC GGGCTGCACG AGGAGATCGA GCATTTCTAT 
CAGTACGTCC TGCCCACGCC CTGCGAACAC GCCATCCGCA ACGAGGTGGT CAAGCGTATC 
GAGGCCGTTG TCCATTCCAT CTGGCCACAG GCAGTAGTCG AGATCTTTGG CTCCTTTCGC 
ACTGGCCTCT TTTTGCCCAC CTCCGACATA GATCTCGTTG TGTTAGGTTT GTGGGAGAAG 
CTGCCGCTGA GAACGCTGGA GTTCGAGCTA GTGAGCCGTG GTATCGCCGA GGCTTGTACG 
GTGCGTGTTC TGGACAAGGC ATCAGTGCCC ATCATCAAGC TGACGGACCG GGAAACGCAA 
GTGAAGGTAG ACATATCGTT CAACATGCAG AGTGGTGTTC AGTCGGCGGA GCTGATTAAG 
AAGTTTAAGC GCGACTATCC AGTGCTGGAG AAGCTAGTGC TTGTGCTGAA GCAGTTCTTA 
CTACTTCGCG ATCTCAACGA GGTGTTCACG GGCGGCATCT CCTCCTACTC GCTGATCCTT 
ATGTGCATCA GCTTCCTGCA GATGCATCCG CGCGGTATCT ACCACGATAC AGCCAATCTG 
GGGGTGCTTC TCCTCGAGTT CTTCGAACTT TATGGACGTC GCTTCAACTA CATGAAGATC 
GGCATCTCCA TAAAGAACGG TGGCCGCTAC ATGCCCAAGG ATGAGCTGCA GCGTGACATG 
GTCGATGGCC ATCGACCCTC GCTCCTGTGC ATCGAGGATC CGCTGACGCC AGGCAATGAT 
ATCGGTCGAA GCAGCTACGG CGTCTTCCAG GTGCAACAGG CATTCAAGTG CGCCTACCGC 
GTGCTCGCCC TTGCCGTCAG TCCGCTCAAC CTTCTCGGCA TCGATCCGCG CGTCAACTCG 
ATACTGGGTC GAATCATTCA CATCACCGAC GACGTGATCG ATTATCGCGA ATGGATCCGC 
GAGAACTTCG AACATCTGGT GGTGGTGGAT CGTATCTCGC CGCTGCCCAC TGCCGCTCCC 
ACTGCGTACG CCACCGCCAA TGGGGCGCCA AAATATGTGA TTATGCCCTC GGGCGCCGTT 
GTCCAGCAGC TGTACCATCA TCCGGTGCAG GTGCCCACCG CTCACGGGCA CAGCCATGCG 
CACAGCCACA GTCACGGGCA CGCGCATCCT GGCGCCCATC TGTGCCAGCC GTATGTGACC 
GGGACCACTG TATCTGCTGT AACGACGACT ACAACGATGG CGGTGGTGAC CGTGGGCGTG 
TCAGCCGGCG GAGTCCAACA GCAGCAACAG CAACAGAATG CGACGGCCCA TACCCACTCG 
CAACAGCAGA CACAGAACCA ATCGCAGTCG CGCCATAGAC GCGGCAGCAC CTCGTCGGGC 
GATGACTCCG AGGATAGCAA GGACGGCGAT GTTGTCGAGA CGACGTCGAG TGCTCAGGAG 
GTCGTTGACA TCGCGCTATC GACGCCAAAT GGCCTGGCCA ACATGTCCAT GCCCATGCCA 
GTGCATGCTG TCGGAATGCC TGCGTCGAAC AGTTGGAGCG GTAATGGCAA TGGAAACGGA 
AACAGCAGCT CATCCACGGG CTCGTCGCCG GAAATCGCAC ACATCGCTGC CCAGGAAATG 
GACCCCGAGC TTGAGGATCA ACAGCAACAA CAGCAGCACC AAGAGACTTC TGGAGGTAAT 
GGCTTCATCC GACCGGGTGA CGTCGGCACC GGTTCCAACA GGGGAGGCGG TGACGGCAGT 
GGCGGCCGCA ATTACAACCA GCGGAACAAC CACAACTCCA GCGGCTACTA CCACCAGCAG 
TACTACGTGC CACCGCCGAT GCAACAGCAG CTGAGCAAGT CCAACTCCTC CTCCAACTAT 
CACCAGCAAC ACCATCACAG CCACAGCCAT GGCAATCATA GTCACCGACA ACAGCATCAC 
CACCAGCAGC AGCACCACCA CCAGCAGCGG CCGCAGCATC TGCGTGTGGG CGGCGGCAAT 
CGATACCAGA AATCGTTGGG CGGATCGCCC ATCATCAGTG CTGGCAATGC GTCGAACAGC 
AGCAGTAATT GCAGCAACAG CAGCAGCAGC AGCGGCAGCA ACAACAGCCG ACTGCCGCCG 
CTAAGGGGCA CGCTGGTCAA CTCATCGTCA GCAATATCAA TCATTTCCAT ATCCTCAGAA 
TCATCCATTG CTAGCAGCAG CTCCAGTTCG TCAAGATCCG GTCAGGATCA GCAGCGTGAC 
GAGCGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trf4-1 ( NM_001103444.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001096914.1 Drosophila melanogaster topoisomerase related function 4-1, transcript variant G (Trf4-1), mRNA.

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CloneID	OFa01134
Clone ID Related Accession (Same CDS sequence)	NM_001103443.2 , NM_001103444.2
Accession Version	NM_001103444.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	topoisomerase related function 4-1, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_001103444.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCGACA CCAGCAACAA CCCGCCACAA TCAACGACCA CGCCCGCCAG CCGCACGAAC 
AGCATCTACT ACAATCCATC GCGGAAGAAA CGACCGGAGA ACAAGGCTGG CGGAGCCCAC 
TACTATATGA ACAACCATAT GGAGATGATC GCCAAGTACA AGGGCGAACC GTGGCGCAAG 
CCGGACTATC CGTATGGCGA GGGCGTTATC GGGCTGCACG AGGAGATCGA GCATTTCTAT 
CAGTACGTCC TGCCCACGCC CTGCGAACAC GCCATCCGCA ACGAGGTGGT CAAGCGTATC 
GAGGCCGTTG TCCATTCCAT CTGGCCACAG GCAGTAGTCG AGATCTTTGG CTCCTTTCGC 
ACTGGCCTCT TTTTGCCCAC CTCCGACATA GATCTCGTTG TGTTAGGTTT GTGGGAGAAG 
CTGCCGCTGA GAACGCTGGA GTTCGAGCTA GTGAGCCGTG GTATCGCCGA GGCTTGTACG 
GTGCGTGTTC TGGACAAGGC ATCAGTGCCC ATCATCAAGC TGACGGACCG GGAAACGCAA 
GTGAAGGTAG ACATATCGTT CAACATGCAG AGTGGTGTTC AGTCGGCGGA GCTGATTAAG 
AAGTTTAAGC GCGACTATCC AGTGCTGGAG AAGCTAGTGC TTGTGCTGAA GCAGTTCTTA 
CTACTTCGCG ATCTCAACGA GGTGTTCACG GGCGGCATCT CCTCCTACTC GCTGATCCTT 
ATGTGCATCA GCTTCCTGCA GATGCATCCG CGCGGTATCT ACCACGATAC AGCCAATCTG 
GGGGTGCTTC TCCTCGAGTT CTTCGAACTT TATGGACGTC GCTTCAACTA CATGAAGATC 
GGCATCTCCA TAAAGAACGG TGGCCGCTAC ATGCCCAAGG ATGAGCTGCA GCGTGACATG 
GTCGATGGCC ATCGACCCTC GCTCCTGTGC ATCGAGGATC CGCTGACGCC AGGCAATGAT 
ATCGGTCGAA GCAGCTACGG CGTCTTCCAG GTGCAACAGG CATTCAAGTG CGCCTACCGC 
GTGCTCGCCC TTGCCGTCAG TCCGCTCAAC CTTCTCGGCA TCGATCCGCG CGTCAACTCG 
ATACTGGGTC GAATCATTCA CATCACCGAC GACGTGATCG ATTATCGCGA ATGGATCCGC 
GAGAACTTCG AACATCTGGT GGTGGTGGAT CGTATCTCGC CGCTGCCCAC TGCCGCTCCC 
ACTGCGTACG CCACCGCCAA TGGGGCGCCA AAATATGTGA TTATGCCCTC GGGCGCCGTT 
GTCCAGCAGC TGTACCATCA TCCGGTGCAG GTGCCCACCG CTCACGGGCA CAGCCATGCG 
CACAGCCACA GTCACGGGCA CGCGCATCCT GGCGCCCATC TGTGCCAGCC GTATGTGACC 
GGGACCACTG TATCTGCTGT AACGACGACT ACAACGATGG CGGTGGTGAC CGTGGGCGTG 
TCAGCCGGCG GAGTCCAACA GCAGCAACAG CAACAGAATG CGACGGCCCA TACCCACTCG 
CAACAGCAGA CACAGAACCA ATCGCAGTCG CGCCATAGAC GCGGCAGCAC CTCGTCGGGC 
GATGACTCCG AGGATAGCAA GGACGGCGAT GTTGTCGAGA CGACGTCGAG TGCTCAGGAG 
GTCGTTGACA TCGCGCTATC GACGCCAAAT GGCCTGGCCA ACATGTCCAT GCCCATGCCA 
GTGCATGCTG TCGGAATGCC TGCGTCGAAC AGTTGGAGCG GTAATGGCAA TGGAAACGGA 
AACAGCAGCT CATCCACGGG CTCGTCGCCG GAAATCGCAC ACATCGCTGC CCAGGAAATG 
GACCCCGAGC TTGAGGATCA ACAGCAACAA CAGCAGCACC AAGAGACTTC TGGAGGTAAT 
GGCTTCATCC GACCGGGTGA CGTCGGCACC GGTTCCAACA GGGGAGGCGG TGACGGCAGT 
GGCGGCCGCA ATTACAACCA GCGGAACAAC CACAACTCCA GCGGCTACTA CCACCAGCAG 
TACTACGTGC CACCGCCGAT GCAACAGCAG CTGAGCAAGT CCAACTCCTC CTCCAACTAT 
CACCAGCAAC ACCATCACAG CCACAGCCAT GGCAATCATA GTCACCGACA ACAGCATCAC 
CACCAGCAGC AGCACCACCA CCAGCAGCGG CCGCAGCATC TGCGTGTGGG CGGCGGCAAT 
CGATACCAGA AATCGTTGGG CGGATCGCCC ATCATCAGTG CTGGCAATGC GTCGAACAGC 
AGCAGTAATT GCAGCAACAG CAGCAGCAGC AGCGGCAGCA ACAACAGCCG ACTGCCGCCG 
CTAAGGGGCA CGCTGGTCAA CTCATCGTCA GCAATATCAA TCATTTCCAT ATCCTCAGAA 
TCATCCATTG CTAGCAGCAG CTCCAGTTCG TCAAGATCCG GTCAGGATCA GCAGCGTGAC 
GAGCGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001096914.1
CDS	878..3286
Translation	MSDTSNNPPQSTTTPASRTNSIYYNPSRKKRPENKAGGAHYYMNNHMEMIAKYKGEPWRKPDYPYGEGVIGLHEEIEHFYQYVLPTPCEHAIRNEVVKRIEAVVHSIWPQAVVEIFGSFRTGLFLPTSDIDLVVLGLWEKLPLRTLEFELVSRGIAEACTVRVLDKASVPIIKLTDRETQVKVDISFNMQSGVQSAELIKKFKRDYPVLEKLVLVLKQFLLLRDLNEVFTGGISSYSLILMCISFLQMHPRGIYHDTANLGVLLLEFFELYGRRFNYMKIGISIKNGGRYMPKDELQRDMVDGHRPSLLCIEDPLTPGNDIGRSSYGVFQVQQAFKCAYRVLALAVSPLNLLGIDPRVNSILGRIIHITDDVIDYREWIRENFEHLVVVDRISPLPTAAPTAYATANGAPKYVIMPSGAVVQQLYHHPVQVPTAHGHSHAHSHSHGHAHPGAHLCQPYVTGTTVSAVTTTTTMAVVTVGVSAGGVQQQQQQQNATAHTHSQQQTQNQSQSRHRRGSTSSGDDSEDSKDGDVVETTSSAQEVVDIALSTPNGLANMSMPMPVHAVGMPASNSWSGNGNGNGNSSSSTGSSPEIAHIAAQEMDPELEDQQQQQQHQETSGGNGFIRPGDVGTGSNRGGGDGSGGRNYNQRNNHNSSGYYHQQYYVPPPMQQQLSKSNSSSNYHQQHHHSHSHGNHSHRQQHHHQQQHHHQQRPQHLRVGGGNRYQKSLGGSPIISAGNASNSSSNCSNSSSSSGSNNSRLPPLRGTLVNSSSAISIISISSESSIASSSSSSSRSGQDQQRDER

Target ORF information:

RefSeq Version	NM_001103444.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster topoisomerase related function 4-1, transcript variant G (Trf4-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103444.2

ORF Insert Sequence:

ATGAGCGACA CCAGCAACAA CCCGCCACAA TCAACGACCA CGCCCGCCAG CCGCACGAAC 
AGCATCTACT ACAATCCATC GCGGAAGAAA CGACCGGAGA ACAAGGCTGG CGGAGCCCAC 
TACTATATGA ACAACCATAT GGAGATGATC GCCAAGTACA AGGGCGAACC GTGGCGCAAG 
CCGGACTATC CGTATGGCGA GGGCGTTATC GGGCTGCACG AGGAGATCGA GCATTTCTAT 
CAGTACGTCC TGCCCACGCC CTGCGAACAC GCCATCCGCA ACGAGGTGGT CAAGCGTATC 
GAGGCCGTTG TCCATTCCAT CTGGCCACAG GCAGTAGTCG AGATCTTTGG CTCCTTTCGC 
ACTGGCCTCT TTTTGCCCAC CTCCGACATA GATCTCGTTG TGTTAGGTTT GTGGGAGAAG 
CTGCCGCTGA GAACGCTGGA GTTCGAGCTA GTGAGCCGTG GTATCGCCGA GGCTTGTACG 
GTGCGTGTTC TGGACAAGGC ATCAGTGCCC ATCATCAAGC TGACGGACCG GGAAACGCAA 
GTGAAGGTAG ACATATCGTT CAACATGCAG AGTGGTGTTC AGTCGGCGGA GCTGATTAAG 
AAGTTTAAGC GCGACTATCC AGTGCTGGAG AAGCTAGTGC TTGTGCTGAA GCAGTTCTTA 
CTACTTCGCG ATCTCAACGA GGTGTTCACG GGCGGCATCT CCTCCTACTC GCTGATCCTT 
ATGTGCATCA GCTTCCTGCA GATGCATCCG CGCGGTATCT ACCACGATAC AGCCAATCTG 
GGGGTGCTTC TCCTCGAGTT CTTCGAACTT TATGGACGTC GCTTCAACTA CATGAAGATC 
GGCATCTCCA TAAAGAACGG TGGCCGCTAC ATGCCCAAGG ATGAGCTGCA GCGTGACATG 
GTCGATGGCC ATCGACCCTC GCTCCTGTGC ATCGAGGATC CGCTGACGCC AGGCAATGAT 
ATCGGTCGAA GCAGCTACGG CGTCTTCCAG GTGCAACAGG CATTCAAGTG CGCCTACCGC 
GTGCTCGCCC TTGCCGTCAG TCCGCTCAAC CTTCTCGGCA TCGATCCGCG CGTCAACTCG 
ATACTGGGTC GAATCATTCA CATCACCGAC GACGTGATCG ATTATCGCGA ATGGATCCGC 
GAGAACTTCG AACATCTGGT GGTGGTGGAT CGTATCTCGC CGCTGCCCAC TGCCGCTCCC 
ACTGCGTACG CCACCGCCAA TGGGGCGCCA AAATATGTGA TTATGCCCTC GGGCGCCGTT 
GTCCAGCAGC TGTACCATCA TCCGGTGCAG GTGCCCACCG CTCACGGGCA CAGCCATGCG 
CACAGCCACA GTCACGGGCA CGCGCATCCT GGCGCCCATC TGTGCCAGCC GTATGTGACC 
GGGACCACTG TATCTGCTGT AACGACGACT ACAACGATGG CGGTGGTGAC CGTGGGCGTG 
TCAGCCGGCG GAGTCCAACA GCAGCAACAG CAACAGAATG CGACGGCCCA TACCCACTCG 
CAACAGCAGA CACAGAACCA ATCGCAGTCG CGCCATAGAC GCGGCAGCAC CTCGTCGGGC 
GATGACTCCG AGGATAGCAA GGACGGCGAT GTTGTCGAGA CGACGTCGAG TGCTCAGGAG 
GTCGTTGACA TCGCGCTATC GACGCCAAAT GGCCTGGCCA ACATGTCCAT GCCCATGCCA 
GTGCATGCTG TCGGAATGCC TGCGTCGAAC AGTTGGAGCG GTAATGGCAA TGGAAACGGA 
AACAGCAGCT CATCCACGGG CTCGTCGCCG GAAATCGCAC ACATCGCTGC CCAGGAAATG 
GACCCCGAGC TTGAGGATCA ACAGCAACAA CAGCAGCACC AAGAGACTTC TGGAGGTAAT 
GGCTTCATCC GACCGGGTGA CGTCGGCACC GGTTCCAACA GGGGAGGCGG TGACGGCAGT 
GGCGGCCGCA ATTACAACCA GCGGAACAAC CACAACTCCA GCGGCTACTA CCACCAGCAG 
TACTACGTGC CACCGCCGAT GCAACAGCAG CTGAGCAAGT CCAACTCCTC CTCCAACTAT 
CACCAGCAAC ACCATCACAG CCACAGCCAT GGCAATCATA GTCACCGACA ACAGCATCAC 
CACCAGCAGC AGCACCACCA CCAGCAGCGG CCGCAGCATC TGCGTGTGGG CGGCGGCAAT 
CGATACCAGA AATCGTTGGG CGGATCGCCC ATCATCAGTG CTGGCAATGC GTCGAACAGC 
AGCAGTAATT GCAGCAACAG CAGCAGCAGC AGCGGCAGCA ACAACAGCCG ACTGCCGCCG 
CTAAGGGGCA CGCTGGTCAA CTCATCGTCA GCAATATCAA TCATTTCCAT ATCCTCAGAA 
TCATCCATTG CTAGCAGCAG CTCCAGTTCG TCAAGATCCG GTCAGGATCA GCAGCGTGAC 
GAGCGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Trf4-1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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