CG32259 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG32259
Entrez Gene ID	317942
Full Name	CG32259 gene product from transcript CG32259-RD
Synonyms	Dmel\CG32259,NEST:bs27e11
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 64A5-64A5

mRNA and Protein(s)

mRNA	Protein	Name
NM_001169870.1	NP_001163341.1	uncharacterized protein, isoform C
NM_168054.2	NP_728929.2	uncharacterized protein, isoform D

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG32259 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG32259 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa19536	NM_168054.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant D (CG32259), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OFa19536	NM_001169870.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant C (CG32259), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG32259 ( NM_001169870.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163341.1 Drosophila melanogaster uncharacterized protein, transcript variant C (CG32259), mRNA.

Price & Availability↑

CloneID	OFa19536
Clone ID Related Accession (Same CDS sequence)	NM_001169870.1 , NM_168054.2
Accession Version	NM_001169870.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATAAGA TGAACCCAAA TCGTAGCAAT AAGGATTCGG ACACCACTGA GGATTCCAAC 
GCGAAGAAGA GTTTGCAAAT GGATTCCGAT TCATCAGACG TGTCGAAAGA TTACTGTGAT 
GGTTCAGATG AGAACGAGGA GTCCGACCCA TCATCAGGTT CCTCACAGGA TTCGATAACT 
GAAAAATTAG CGAAAGCCAT ATTAGAGGCC AATCTAAGCG AGGACTCTGA CTCTTCATCG 
GGTTCTTCAG AGCACTCTCC AGCTCAAGAA ATACCAATAC CAGAAGCCTT GTTGGAAGTG 
GATCCAAATG ATCAGCAGTC GGTTGTCAAT GCAGTCGGAC TTTTGGTTAA CATGATTAAC 
TCCAAACTGG ATGCTATCAT GTCGAAAGTC GTCCCGCAAT CACCACTTGA TCTTGTCGAC 
CTCGTCCGCC AGAAAAAACT TTGGAAGATG CGTCAACGCG ATAAAGCCCA GTTGAAGAAA 
AGCTCCGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163341.1
CDS	141..632
Translation	MNKMNPNRSNKDSDTTEDSNAKKSLQMDSDSSDVSKDYCDGSDENEESDPSSGSSQDSITEKLAKAILEANLSEDSDSSSGSSEHSPAQEIPIPEALLEVDPNDQQSVVNAVGLLVNMINSKLDAIMSKVVPQSPLDLVDLVRQKKLWKMRQRDKAQLKKSSE

Target ORF information:

RefSeq Version	NM_001169870.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant C (CG32259), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169870.1

ORF Insert Sequence:

ATGAATAAGA TGAACCCAAA TCGTAGCAAT AAGGATTCGG ACACCACTGA GGATTCCAAC 
GCGAAGAAGA GTTTGCAAAT GGATTCCGAT TCATCAGACG TGTCGAAAGA TTACTGTGAT 
GGTTCAGATG AGAACGAGGA GTCCGACCCA TCATCAGGTT CCTCACAGGA TTCGATAACT 
GAAAAATTAG CGAAAGCCAT ATTAGAGGCC AATCTAAGCG AGGACTCTGA CTCTTCATCG 
GGTTCTTCAG AGCACTCTCC AGCTCAAGAA ATACCAATAC CAGAAGCCTT GTTGGAAGTG 
GATCCAAATG ATCAGCAGTC GGTTGTCAAT GCAGTCGGAC TTTTGGTTAA CATGATTAAC 
TCCAAACTGG ATGCTATCAT GTCGAAAGTC GTCCCGCAAT CACCACTTGA TCTTGTCGAC 
CTCGTCCGCC AGAAAAAACT TTGGAAGATG CGTCAACGCG ATAAAGCCCA GTTGAAGAAA 
AGCTCCGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG32259 ( NM_168054.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728929.2 Drosophila melanogaster uncharacterized protein, transcript variant D (CG32259), mRNA.

Price & Availability↑

CloneID	OFa19536
Clone ID Related Accession (Same CDS sequence)	NM_001169870.1 , NM_168054.2
Accession Version	NM_168054.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Dec 17, 2009 this sequence version replaced NM_168054.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAATAAGA TGAACCCAAA TCGTAGCAAT AAGGATTCGG ACACCACTGA GGATTCCAAC 
GCGAAGAAGA GTTTGCAAAT GGATTCCGAT TCATCAGACG TGTCGAAAGA TTACTGTGAT 
GGTTCAGATG AGAACGAGGA GTCCGACCCA TCATCAGGTT CCTCACAGGA TTCGATAACT 
GAAAAATTAG CGAAAGCCAT ATTAGAGGCC AATCTAAGCG AGGACTCTGA CTCTTCATCG 
GGTTCTTCAG AGCACTCTCC AGCTCAAGAA ATACCAATAC CAGAAGCCTT GTTGGAAGTG 
GATCCAAATG ATCAGCAGTC GGTTGTCAAT GCAGTCGGAC TTTTGGTTAA CATGATTAAC 
TCCAAACTGG ATGCTATCAT GTCGAAAGTC GTCCCGCAAT CACCACTTGA TCTTGTCGAC 
CTCGTCCGCC AGAAAAAACT TTGGAAGATG CGTCAACGCG ATAAAGCCCA GTTGAAGAAA 
AGCTCCGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728929.2
CDS	189..680
Translation	MNKMNPNRSNKDSDTTEDSNAKKSLQMDSDSSDVSKDYCDGSDENEESDPSSGSSQDSITEKLAKAILEANLSEDSDSSSGSSEHSPAQEIPIPEALLEVDPNDQQSVVNAVGLLVNMINSKLDAIMSKVVPQSPLDLVDLVRQKKLWKMRQRDKAQLKKSSE

Target ORF information:

RefSeq Version	NM_168054.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant D (CG32259), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_168054.2

ORF Insert Sequence:

ATGAATAAGA TGAACCCAAA TCGTAGCAAT AAGGATTCGG ACACCACTGA GGATTCCAAC 
GCGAAGAAGA GTTTGCAAAT GGATTCCGAT TCATCAGACG TGTCGAAAGA TTACTGTGAT 
GGTTCAGATG AGAACGAGGA GTCCGACCCA TCATCAGGTT CCTCACAGGA TTCGATAACT 
GAAAAATTAG CGAAAGCCAT ATTAGAGGCC AATCTAAGCG AGGACTCTGA CTCTTCATCG 
GGTTCTTCAG AGCACTCTCC AGCTCAAGAA ATACCAATAC CAGAAGCCTT GTTGGAAGTG 
GATCCAAATG ATCAGCAGTC GGTTGTCAAT GCAGTCGGAC TTTTGGTTAA CATGATTAAC 
TCCAAACTGG ATGCTATCAT GTCGAAAGTC GTCCCGCAAT CACCACTTGA TCTTGTCGAC 
CTCGTCCGCC AGAAAAAACT TTGGAAGATG CGTCAACGCG ATAAAGCCCA GTTGAAGAAA 
AGCTCCGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG32259 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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