CG2258 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG2258
Entrez Gene ID	31729
Full Name	CG2258 gene product from transcript CG2258-RD
Synonyms	Dmel\CG2258
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 7D6-7D6

mRNA and Protein(s)

mRNA	Protein	Name
NM_206649.2	NP_996372.1	uncharacterized protein, isoform B
NM_206648.2	NP_996371.1	uncharacterized protein, isoform C
NM_132211.2	NP_572439.1	uncharacterized protein, isoform A
NM_001298071.1	NP_001285000.1	uncharacterized protein, isoform D

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG2258 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG2258 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01075	NM_132211.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG2258), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa01075	NM_206649.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG2258), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa01075	NM_206648.2 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant C (CG2258), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa01075	NM_001298071.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant D (CG2258), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG2258 ( NM_206649.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996372.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG2258), mRNA.

Price & Availability↑

CloneID	OFa01075
Clone ID Related Accession (Same CDS sequence)	NM_206649.2 , NM_206648.2 , NM_132211.2 , NM_001298071.1
Accession Version	NM_206649.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_206649.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTCAAGG CTCTGTACGA CTTCCAGGCG GTCTATCCGA AGACCATCAG CTTCGATGAG 
GGCGAGTACT TCATCCTTTA CCAGACGTCC GCCCGCCAGC GCAATTGGTG GCAGGTTGTC 
AGCATGAAAG GCAACATTGG CTTTGTGCCC TCCAATTACG TAATGAAGAT TAAGGTGGAG 
CACGACTTTC TCATCAGCTT TCTGAACTCC TCGATAGAAT CGCTGGAGAA GTGCACGGAC 
CATGAGATCA ATGGCATCAT GTCCAAGGAC GAACTGCTGG ACAGGCTGCG CGAGAAGAAG 
CACACCATGG AGCGTCTGTA TGCGGAGAGT TCCGAGCGCG ACGGTGACTC CTCGCTTTCC 
TATTCACACA GCTACAGTGA CAAGGGTAGC CACCGGCACT CGCATCCCCA TCCCACCCAA 
TCCCAGACGC AGCTTCATTC CCAGCACAGG CAGCACAGTC CGCCGCCCAG CGGCGGCAGC 
GTTGGCTCCC AGCGTTTGGA CATGAGCAAG AAGAGCATGT CAAGTCCAGC GGTGGGCTCA 
TCGTGCGGCC TGCCCCAGAA TCAGGGCATG ATCGAATCGC CCAGCATGGG CAGCATGCAG 
TTCCAGGGCA GCAGCTGCAC CATCCAGACG CCGCAGCAGC AACAGCCGCT CCCAGCACCG 
CCAGCACCAG CTCCCTCGCC ATCGGCAGCA TCGGCCACAC CCACTGCGGC ATCAACGAGC 
AAAGCGGCCA CCGGCGGTGA TGTCGCGCAG GACAATAGCA TTACGTCGGA GCCCTCGGAG 
ACGACAACGA CCACTACGAC GACCAGCGAA GATGTGGTCA CCACATACAA GGAGACCAGC 
CAAATGAGCA CCAGCCAGCA GCATAAGCCG CAAAATGGGA GCACTGCCTC GGCGTCCATT 
TCCGCATCTG CCTCGGCCTC AGCTTTGCGC AGCAACGGCG GCAACGGCAA GGCCAACGGT 
AGTGCCACAG CCCTGCATCA GGAGCTGCAG CAGGAGGAAG CGGACAGTGC GCCGGACAAG 
TCGGATGATG CCAGCGCCGT GCAAAGTGAC GACGGTTGCC CGGCCAACGG AGATAGTGCG 
GATAATAGCC AGGCGCTGGA CAGCATCGAC AGTCCGTCGC ATCGACAGCG CGGCCTGAGC 
CTGGGCAGAA TCGAGGAGGG TGCCACAGGA GGAGGCCAGT TGAAGGTTGA GTCATCGGAT 
GTATATCAGA TTGTGGACGC CCTGCGGCGG AACACCAACC TCAGCTTCGA TCTCTCCTGT 
GAAGCATTGC GCGTGGTGCT GACCAGCCTG GAGCAGCTGT ACAACGGTGC CATCAATCCG 
TACCTGGAGG CGGTGGCCGT TCATGTCACT GGCAAAGTGG CCACGCCCAA GGAGCTGCTG 
GGCATTACGC ACGACGCCAA GCGCTTGCAG TATCTCTTTG CCCAGCTGGC GGACTGCAAG 
AACGATACGG AGCAACGCAC TTGGATGCTC TACGAGGATG AGGAGGACAT CATTCAGTTC 
CTTGAGGAGC TTGTTGAGAT TTTGATCAAT GCTGATGAGA GCATCAGTTG CTACGAGATG 
TCCTGCGATC AGTACCAGAT GTTTATCAAT CTGGTGCAGT ACTATCAGAT GGAGACGCGC 
TGGTCCATCA AGCGACTGCT GCTTAAGACC TTTACGGCCG CTTGCCATCT GGACTATATC 
ATTGTGGACA TATTGCTGAC CTCGGTGCTG CCATTGGAGA TTGTCGAGGA CATGAAGACG 
CACTTCTCCA ATCTGGATCG CTTCAAGCAG CTGGTCAAGA TGCTCACTAT TATCTTCTCA 
CTTGGACAGC CCATGCCGGT TAATCATCAG GATTATTTGG GCGTACATTT TGCCAGCTTC 
CTACTGGAAA TCGTCGAGGG CAATAATCCG GAGGTCTTGG TGGACATGGT CATTGCCTTG 
ATCTTGGCCT TCAATCAGCA ATTTAGCGAA CATACCTATA ATGTCATCAT CGAAGGTATG 
CAGAATCTGC CATCCGCCAA AGTGTTTACG GAGAAGTTGC TACTTTTGCT CAATCGAGAG 
GATGATCCCA CACGCTTGCT CAAGCATCCC AACGAGCATA TGAACACGGT GCTGCGAATG 
TTTATCGACA TATTCAGCCA TCCGGATACG GCGGGCATGT TCTACACGAA TGACATCAAG 
GTGCTTATCG ATATAGTGGT TCGCCAGCTA TCCGATTTGG ATGCCGGCAG TACGACGCGA 
CCATGCTACT TGGAGCTTTG CCGACGCATC CTGCGCAATA CGAACTATCA GGAGCACCAG 
CATCGCAAGC ATGATCTCAT GAAGATCTTC ACGCGCATCT TCTGCGAGGA GACCGAGTGC 
AGTGCCTCCG ATCAGCAGCT GGTGCGGGAA ATAGCGAACG AATTTCCGCA GCTGTTCAAG 
GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996372.1
CDS	456..2861
Translation	MLKALYDFQAVYPKTISFDEGEYFILYQTSARQRNWWQVVSMKGNIGFVPSNYVMKIKVEHDFLISFLNSSIESLEKCTDHEINGIMSKDELLDRLREKKHTMERLYAESSERDGDSSLSYSHSYSDKGSHRHSHPHPTQSQTQLHSQHRQHSPPPSGGSVGSQRLDMSKKSMSSPAVGSSCGLPQNQGMIESPSMGSMQFQGSSCTIQTPQQQQPLPAPPAPAPSPSAASATPTAASTSKAATGGDVAQDNSITSEPSETTTTTTTTSEDVVTTYKETSQMSTSQQHKPQNGSTASASISASASASALRSNGGNGKANGSATALHQELQQEEADSAPDKSDDASAVQSDDGCPANGDSADNSQALDSIDSPSHRQRGLSLGRIEEGATGGGQLKVESSDVYQIVDALRRNTNLSFDLSCEALRVVLTSLEQLYNGAINPYLEAVAVHVTGKVATPKELLGITHDAKRLQYLFAQLADCKNDTEQRTWMLYEDEEDIIQFLEELVEILINADESISCYEMSCDQYQMFINLVQYYQMETRWSIKRLLLKTFTAACHLDYIIVDILLTSVLPLEIVEDMKTHFSNLDRFKQLVKMLTIIFSLGQPMPVNHQDYLGVHFASFLLEIVEGNNPEVLVDMVIALILAFNQQFSEHTYNVIIEGMQNLPSAKVFTEKLLLLLNREDDPTRLLKHPNEHMNTVLRMFIDIFSHPDTAGMFYTNDIKVLIDIVVRQLSDLDAGSTTRPCYLELCRRILRNTNYQEHQHRKHDLMKIFTRIFCEETECSASDQQLVREIANEFPQLFKA

Target ORF information:

RefSeq Version	NM_206649.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG2258), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206649.2

ORF Insert Sequence:

ATGCTCAAGG CTCTGTACGA CTTCCAGGCG GTCTATCCGA AGACCATCAG CTTCGATGAG 
GGCGAGTACT TCATCCTTTA CCAGACGTCC GCCCGCCAGC GCAATTGGTG GCAGGTTGTC 
AGCATGAAAG GCAACATTGG CTTTGTGCCC TCCAATTACG TAATGAAGAT TAAGGTGGAG 
CACGACTTTC TCATCAGCTT TCTGAACTCC TCGATAGAAT CGCTGGAGAA GTGCACGGAC 
CATGAGATCA ATGGCATCAT GTCCAAGGAC GAACTGCTGG ACAGGCTGCG CGAGAAGAAG 
CACACCATGG AGCGTCTGTA TGCGGAGAGT TCCGAGCGCG ACGGTGACTC CTCGCTTTCC 
TATTCACACA GCTACAGTGA CAAGGGTAGC CACCGGCACT CGCATCCCCA TCCCACCCAA 
TCCCAGACGC AGCTTCATTC CCAGCACAGG CAGCACAGTC CGCCGCCCAG CGGCGGCAGC 
GTTGGCTCCC AGCGTTTGGA CATGAGCAAG AAGAGCATGT CAAGTCCAGC GGTGGGCTCA 
TCGTGCGGCC TGCCCCAGAA TCAGGGCATG ATCGAATCGC CCAGCATGGG CAGCATGCAG 
TTCCAGGGCA GCAGCTGCAC CATCCAGACG CCGCAGCAGC AACAGCCGCT CCCAGCACCG 
CCAGCACCAG CTCCCTCGCC ATCGGCAGCA TCGGCCACAC CCACTGCGGC ATCAACGAGC 
AAAGCGGCCA CCGGCGGTGA TGTCGCGCAG GACAATAGCA TTACGTCGGA GCCCTCGGAG 
ACGACAACGA CCACTACGAC GACCAGCGAA GATGTGGTCA CCACATACAA GGAGACCAGC 
CAAATGAGCA CCAGCCAGCA GCATAAGCCG CAAAATGGGA GCACTGCCTC GGCGTCCATT 
TCCGCATCTG CCTCGGCCTC AGCTTTGCGC AGCAACGGCG GCAACGGCAA GGCCAACGGT 
AGTGCCACAG CCCTGCATCA GGAGCTGCAG CAGGAGGAAG CGGACAGTGC GCCGGACAAG 
TCGGATGATG CCAGCGCCGT GCAAAGTGAC GACGGTTGCC CGGCCAACGG AGATAGTGCG 
GATAATAGCC AGGCGCTGGA CAGCATCGAC AGTCCGTCGC ATCGACAGCG CGGCCTGAGC 
CTGGGCAGAA TCGAGGAGGG TGCCACAGGA GGAGGCCAGT TGAAGGTTGA GTCATCGGAT 
GTATATCAGA TTGTGGACGC CCTGCGGCGG AACACCAACC TCAGCTTCGA TCTCTCCTGT 
GAAGCATTGC GCGTGGTGCT GACCAGCCTG GAGCAGCTGT ACAACGGTGC CATCAATCCG 
TACCTGGAGG CGGTGGCCGT TCATGTCACT GGCAAAGTGG CCACGCCCAA GGAGCTGCTG 
GGCATTACGC ACGACGCCAA GCGCTTGCAG TATCTCTTTG CCCAGCTGGC GGACTGCAAG 
AACGATACGG AGCAACGCAC TTGGATGCTC TACGAGGATG AGGAGGACAT CATTCAGTTC 
CTTGAGGAGC TTGTTGAGAT TTTGATCAAT GCTGATGAGA GCATCAGTTG CTACGAGATG 
TCCTGCGATC AGTACCAGAT GTTTATCAAT CTGGTGCAGT ACTATCAGAT GGAGACGCGC 
TGGTCCATCA AGCGACTGCT GCTTAAGACC TTTACGGCCG CTTGCCATCT GGACTATATC 
ATTGTGGACA TATTGCTGAC CTCGGTGCTG CCATTGGAGA TTGTCGAGGA CATGAAGACG 
CACTTCTCCA ATCTGGATCG CTTCAAGCAG CTGGTCAAGA TGCTCACTAT TATCTTCTCA 
CTTGGACAGC CCATGCCGGT TAATCATCAG GATTATTTGG GCGTACATTT TGCCAGCTTC 
CTACTGGAAA TCGTCGAGGG CAATAATCCG GAGGTCTTGG TGGACATGGT CATTGCCTTG 
ATCTTGGCCT TCAATCAGCA ATTTAGCGAA CATACCTATA ATGTCATCAT CGAAGGTATG 
CAGAATCTGC CATCCGCCAA AGTGTTTACG GAGAAGTTGC TACTTTTGCT CAATCGAGAG 
GATGATCCCA CACGCTTGCT CAAGCATCCC AACGAGCATA TGAACACGGT GCTGCGAATG 
TTTATCGACA TATTCAGCCA TCCGGATACG GCGGGCATGT TCTACACGAA TGACATCAAG 
GTGCTTATCG ATATAGTGGT TCGCCAGCTA TCCGATTTGG ATGCCGGCAG TACGACGCGA 
CCATGCTACT TGGAGCTTTG CCGACGCATC CTGCGCAATA CGAACTATCA GGAGCACCAG 
CATCGCAAGC ATGATCTCAT GAAGATCTTC ACGCGCATCT TCTGCGAGGA GACCGAGTGC 
AGTGCCTCCG ATCAGCAGCT GGTGCGGGAA ATAGCGAACG AATTTCCGCA GCTGTTCAAG 
GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG2258 ( NM_206648.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_996371.1 Drosophila melanogaster uncharacterized protein, transcript variant C (CG2258), mRNA.

Price & Availability↑

CloneID	OFa01075
Clone ID Related Accession (Same CDS sequence)	NM_206649.2 , NM_206648.2 , NM_132211.2 , NM_001298071.1
Accession Version	NM_206648.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_206648.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTCAAGG CTCTGTACGA CTTCCAGGCG GTCTATCCGA AGACCATCAG CTTCGATGAG 
GGCGAGTACT TCATCCTTTA CCAGACGTCC GCCCGCCAGC GCAATTGGTG GCAGGTTGTC 
AGCATGAAAG GCAACATTGG CTTTGTGCCC TCCAATTACG TAATGAAGAT TAAGGTGGAG 
CACGACTTTC TCATCAGCTT TCTGAACTCC TCGATAGAAT CGCTGGAGAA GTGCACGGAC 
CATGAGATCA ATGGCATCAT GTCCAAGGAC GAACTGCTGG ACAGGCTGCG CGAGAAGAAG 
CACACCATGG AGCGTCTGTA TGCGGAGAGT TCCGAGCGCG ACGGTGACTC CTCGCTTTCC 
TATTCACACA GCTACAGTGA CAAGGGTAGC CACCGGCACT CGCATCCCCA TCCCACCCAA 
TCCCAGACGC AGCTTCATTC CCAGCACAGG CAGCACAGTC CGCCGCCCAG CGGCGGCAGC 
GTTGGCTCCC AGCGTTTGGA CATGAGCAAG AAGAGCATGT CAAGTCCAGC GGTGGGCTCA 
TCGTGCGGCC TGCCCCAGAA TCAGGGCATG ATCGAATCGC CCAGCATGGG CAGCATGCAG 
TTCCAGGGCA GCAGCTGCAC CATCCAGACG CCGCAGCAGC AACAGCCGCT CCCAGCACCG 
CCAGCACCAG CTCCCTCGCC ATCGGCAGCA TCGGCCACAC CCACTGCGGC ATCAACGAGC 
AAAGCGGCCA CCGGCGGTGA TGTCGCGCAG GACAATAGCA TTACGTCGGA GCCCTCGGAG 
ACGACAACGA CCACTACGAC GACCAGCGAA GATGTGGTCA CCACATACAA GGAGACCAGC 
CAAATGAGCA CCAGCCAGCA GCATAAGCCG CAAAATGGGA GCACTGCCTC GGCGTCCATT 
TCCGCATCTG CCTCGGCCTC AGCTTTGCGC AGCAACGGCG GCAACGGCAA GGCCAACGGT 
AGTGCCACAG CCCTGCATCA GGAGCTGCAG CAGGAGGAAG CGGACAGTGC GCCGGACAAG 
TCGGATGATG CCAGCGCCGT GCAAAGTGAC GACGGTTGCC CGGCCAACGG AGATAGTGCG 
GATAATAGCC AGGCGCTGGA CAGCATCGAC AGTCCGTCGC ATCGACAGCG CGGCCTGAGC 
CTGGGCAGAA TCGAGGAGGG TGCCACAGGA GGAGGCCAGT TGAAGGTTGA GTCATCGGAT 
GTATATCAGA TTGTGGACGC CCTGCGGCGG AACACCAACC TCAGCTTCGA TCTCTCCTGT 
GAAGCATTGC GCGTGGTGCT GACCAGCCTG GAGCAGCTGT ACAACGGTGC CATCAATCCG 
TACCTGGAGG CGGTGGCCGT TCATGTCACT GGCAAAGTGG CCACGCCCAA GGAGCTGCTG 
GGCATTACGC ACGACGCCAA GCGCTTGCAG TATCTCTTTG CCCAGCTGGC GGACTGCAAG 
AACGATACGG AGCAACGCAC TTGGATGCTC TACGAGGATG AGGAGGACAT CATTCAGTTC 
CTTGAGGAGC TTGTTGAGAT TTTGATCAAT GCTGATGAGA GCATCAGTTG CTACGAGATG 
TCCTGCGATC AGTACCAGAT GTTTATCAAT CTGGTGCAGT ACTATCAGAT GGAGACGCGC 
TGGTCCATCA AGCGACTGCT GCTTAAGACC TTTACGGCCG CTTGCCATCT GGACTATATC 
ATTGTGGACA TATTGCTGAC CTCGGTGCTG CCATTGGAGA TTGTCGAGGA CATGAAGACG 
CACTTCTCCA ATCTGGATCG CTTCAAGCAG CTGGTCAAGA TGCTCACTAT TATCTTCTCA 
CTTGGACAGC CCATGCCGGT TAATCATCAG GATTATTTGG GCGTACATTT TGCCAGCTTC 
CTACTGGAAA TCGTCGAGGG CAATAATCCG GAGGTCTTGG TGGACATGGT CATTGCCTTG 
ATCTTGGCCT TCAATCAGCA ATTTAGCGAA CATACCTATA ATGTCATCAT CGAAGGTATG 
CAGAATCTGC CATCCGCCAA AGTGTTTACG GAGAAGTTGC TACTTTTGCT CAATCGAGAG 
GATGATCCCA CACGCTTGCT CAAGCATCCC AACGAGCATA TGAACACGGT GCTGCGAATG 
TTTATCGACA TATTCAGCCA TCCGGATACG GCGGGCATGT TCTACACGAA TGACATCAAG 
GTGCTTATCG ATATAGTGGT TCGCCAGCTA TCCGATTTGG ATGCCGGCAG TACGACGCGA 
CCATGCTACT TGGAGCTTTG CCGACGCATC CTGCGCAATA CGAACTATCA GGAGCACCAG 
CATCGCAAGC ATGATCTCAT GAAGATCTTC ACGCGCATCT TCTGCGAGGA GACCGAGTGC 
AGTGCCTCCG ATCAGCAGCT GGTGCGGGAA ATAGCGAACG AATTTCCGCA GCTGTTCAAG 
GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996371.1
CDS	915..3320
Translation	MLKALYDFQAVYPKTISFDEGEYFILYQTSARQRNWWQVVSMKGNIGFVPSNYVMKIKVEHDFLISFLNSSIESLEKCTDHEINGIMSKDELLDRLREKKHTMERLYAESSERDGDSSLSYSHSYSDKGSHRHSHPHPTQSQTQLHSQHRQHSPPPSGGSVGSQRLDMSKKSMSSPAVGSSCGLPQNQGMIESPSMGSMQFQGSSCTIQTPQQQQPLPAPPAPAPSPSAASATPTAASTSKAATGGDVAQDNSITSEPSETTTTTTTTSEDVVTTYKETSQMSTSQQHKPQNGSTASASISASASASALRSNGGNGKANGSATALHQELQQEEADSAPDKSDDASAVQSDDGCPANGDSADNSQALDSIDSPSHRQRGLSLGRIEEGATGGGQLKVESSDVYQIVDALRRNTNLSFDLSCEALRVVLTSLEQLYNGAINPYLEAVAVHVTGKVATPKELLGITHDAKRLQYLFAQLADCKNDTEQRTWMLYEDEEDIIQFLEELVEILINADESISCYEMSCDQYQMFINLVQYYQMETRWSIKRLLLKTFTAACHLDYIIVDILLTSVLPLEIVEDMKTHFSNLDRFKQLVKMLTIIFSLGQPMPVNHQDYLGVHFASFLLEIVEGNNPEVLVDMVIALILAFNQQFSEHTYNVIIEGMQNLPSAKVFTEKLLLLLNREDDPTRLLKHPNEHMNTVLRMFIDIFSHPDTAGMFYTNDIKVLIDIVVRQLSDLDAGSTTRPCYLELCRRILRNTNYQEHQHRKHDLMKIFTRIFCEETECSASDQQLVREIANEFPQLFKA

Target ORF information:

RefSeq Version	NM_206648.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant C (CG2258), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_206648.2

ORF Insert Sequence:

ATGCTCAAGG CTCTGTACGA CTTCCAGGCG GTCTATCCGA AGACCATCAG CTTCGATGAG 
GGCGAGTACT TCATCCTTTA CCAGACGTCC GCCCGCCAGC GCAATTGGTG GCAGGTTGTC 
AGCATGAAAG GCAACATTGG CTTTGTGCCC TCCAATTACG TAATGAAGAT TAAGGTGGAG 
CACGACTTTC TCATCAGCTT TCTGAACTCC TCGATAGAAT CGCTGGAGAA GTGCACGGAC 
CATGAGATCA ATGGCATCAT GTCCAAGGAC GAACTGCTGG ACAGGCTGCG CGAGAAGAAG 
CACACCATGG AGCGTCTGTA TGCGGAGAGT TCCGAGCGCG ACGGTGACTC CTCGCTTTCC 
TATTCACACA GCTACAGTGA CAAGGGTAGC CACCGGCACT CGCATCCCCA TCCCACCCAA 
TCCCAGACGC AGCTTCATTC CCAGCACAGG CAGCACAGTC CGCCGCCCAG CGGCGGCAGC 
GTTGGCTCCC AGCGTTTGGA CATGAGCAAG AAGAGCATGT CAAGTCCAGC GGTGGGCTCA 
TCGTGCGGCC TGCCCCAGAA TCAGGGCATG ATCGAATCGC CCAGCATGGG CAGCATGCAG 
TTCCAGGGCA GCAGCTGCAC CATCCAGACG CCGCAGCAGC AACAGCCGCT CCCAGCACCG 
CCAGCACCAG CTCCCTCGCC ATCGGCAGCA TCGGCCACAC CCACTGCGGC ATCAACGAGC 
AAAGCGGCCA CCGGCGGTGA TGTCGCGCAG GACAATAGCA TTACGTCGGA GCCCTCGGAG 
ACGACAACGA CCACTACGAC GACCAGCGAA GATGTGGTCA CCACATACAA GGAGACCAGC 
CAAATGAGCA CCAGCCAGCA GCATAAGCCG CAAAATGGGA GCACTGCCTC GGCGTCCATT 
TCCGCATCTG CCTCGGCCTC AGCTTTGCGC AGCAACGGCG GCAACGGCAA GGCCAACGGT 
AGTGCCACAG CCCTGCATCA GGAGCTGCAG CAGGAGGAAG CGGACAGTGC GCCGGACAAG 
TCGGATGATG CCAGCGCCGT GCAAAGTGAC GACGGTTGCC CGGCCAACGG AGATAGTGCG 
GATAATAGCC AGGCGCTGGA CAGCATCGAC AGTCCGTCGC ATCGACAGCG CGGCCTGAGC 
CTGGGCAGAA TCGAGGAGGG TGCCACAGGA GGAGGCCAGT TGAAGGTTGA GTCATCGGAT 
GTATATCAGA TTGTGGACGC CCTGCGGCGG AACACCAACC TCAGCTTCGA TCTCTCCTGT 
GAAGCATTGC GCGTGGTGCT GACCAGCCTG GAGCAGCTGT ACAACGGTGC CATCAATCCG 
TACCTGGAGG CGGTGGCCGT TCATGTCACT GGCAAAGTGG CCACGCCCAA GGAGCTGCTG 
GGCATTACGC ACGACGCCAA GCGCTTGCAG TATCTCTTTG CCCAGCTGGC GGACTGCAAG 
AACGATACGG AGCAACGCAC TTGGATGCTC TACGAGGATG AGGAGGACAT CATTCAGTTC 
CTTGAGGAGC TTGTTGAGAT TTTGATCAAT GCTGATGAGA GCATCAGTTG CTACGAGATG 
TCCTGCGATC AGTACCAGAT GTTTATCAAT CTGGTGCAGT ACTATCAGAT GGAGACGCGC 
TGGTCCATCA AGCGACTGCT GCTTAAGACC TTTACGGCCG CTTGCCATCT GGACTATATC 
ATTGTGGACA TATTGCTGAC CTCGGTGCTG CCATTGGAGA TTGTCGAGGA CATGAAGACG 
CACTTCTCCA ATCTGGATCG CTTCAAGCAG CTGGTCAAGA TGCTCACTAT TATCTTCTCA 
CTTGGACAGC CCATGCCGGT TAATCATCAG GATTATTTGG GCGTACATTT TGCCAGCTTC 
CTACTGGAAA TCGTCGAGGG CAATAATCCG GAGGTCTTGG TGGACATGGT CATTGCCTTG 
ATCTTGGCCT TCAATCAGCA ATTTAGCGAA CATACCTATA ATGTCATCAT CGAAGGTATG 
CAGAATCTGC CATCCGCCAA AGTGTTTACG GAGAAGTTGC TACTTTTGCT CAATCGAGAG 
GATGATCCCA CACGCTTGCT CAAGCATCCC AACGAGCATA TGAACACGGT GCTGCGAATG 
TTTATCGACA TATTCAGCCA TCCGGATACG GCGGGCATGT TCTACACGAA TGACATCAAG 
GTGCTTATCG ATATAGTGGT TCGCCAGCTA TCCGATTTGG ATGCCGGCAG TACGACGCGA 
CCATGCTACT TGGAGCTTTG CCGACGCATC CTGCGCAATA CGAACTATCA GGAGCACCAG 
CATCGCAAGC ATGATCTCAT GAAGATCTTC ACGCGCATCT TCTGCGAGGA GACCGAGTGC 
AGTGCCTCCG ATCAGCAGCT GGTGCGGGAA ATAGCGAACG AATTTCCGCA GCTGTTCAAG 
GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG2258 ( NM_132211.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572439.1 Drosophila melanogaster uncharacterized protein, transcript variant A (CG2258), mRNA.

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CloneID	OFa01075
Clone ID Related Accession (Same CDS sequence)	NM_206649.2 , NM_206648.2 , NM_132211.2 , NM_001298071.1
Accession Version	NM_132211.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_132211.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTCAAGG CTCTGTACGA CTTCCAGGCG GTCTATCCGA AGACCATCAG CTTCGATGAG 
GGCGAGTACT TCATCCTTTA CCAGACGTCC GCCCGCCAGC GCAATTGGTG GCAGGTTGTC 
AGCATGAAAG GCAACATTGG CTTTGTGCCC TCCAATTACG TAATGAAGAT TAAGGTGGAG 
CACGACTTTC TCATCAGCTT TCTGAACTCC TCGATAGAAT CGCTGGAGAA GTGCACGGAC 
CATGAGATCA ATGGCATCAT GTCCAAGGAC GAACTGCTGG ACAGGCTGCG CGAGAAGAAG 
CACACCATGG AGCGTCTGTA TGCGGAGAGT TCCGAGCGCG ACGGTGACTC CTCGCTTTCC 
TATTCACACA GCTACAGTGA CAAGGGTAGC CACCGGCACT CGCATCCCCA TCCCACCCAA 
TCCCAGACGC AGCTTCATTC CCAGCACAGG CAGCACAGTC CGCCGCCCAG CGGCGGCAGC 
GTTGGCTCCC AGCGTTTGGA CATGAGCAAG AAGAGCATGT CAAGTCCAGC GGTGGGCTCA 
TCGTGCGGCC TGCCCCAGAA TCAGGGCATG ATCGAATCGC CCAGCATGGG CAGCATGCAG 
TTCCAGGGCA GCAGCTGCAC CATCCAGACG CCGCAGCAGC AACAGCCGCT CCCAGCACCG 
CCAGCACCAG CTCCCTCGCC ATCGGCAGCA TCGGCCACAC CCACTGCGGC ATCAACGAGC 
AAAGCGGCCA CCGGCGGTGA TGTCGCGCAG GACAATAGCA TTACGTCGGA GCCCTCGGAG 
ACGACAACGA CCACTACGAC GACCAGCGAA GATGTGGTCA CCACATACAA GGAGACCAGC 
CAAATGAGCA CCAGCCAGCA GCATAAGCCG CAAAATGGGA GCACTGCCTC GGCGTCCATT 
TCCGCATCTG CCTCGGCCTC AGCTTTGCGC AGCAACGGCG GCAACGGCAA GGCCAACGGT 
AGTGCCACAG CCCTGCATCA GGAGCTGCAG CAGGAGGAAG CGGACAGTGC GCCGGACAAG 
TCGGATGATG CCAGCGCCGT GCAAAGTGAC GACGGTTGCC CGGCCAACGG AGATAGTGCG 
GATAATAGCC AGGCGCTGGA CAGCATCGAC AGTCCGTCGC ATCGACAGCG CGGCCTGAGC 
CTGGGCAGAA TCGAGGAGGG TGCCACAGGA GGAGGCCAGT TGAAGGTTGA GTCATCGGAT 
GTATATCAGA TTGTGGACGC CCTGCGGCGG AACACCAACC TCAGCTTCGA TCTCTCCTGT 
GAAGCATTGC GCGTGGTGCT GACCAGCCTG GAGCAGCTGT ACAACGGTGC CATCAATCCG 
TACCTGGAGG CGGTGGCCGT TCATGTCACT GGCAAAGTGG CCACGCCCAA GGAGCTGCTG 
GGCATTACGC ACGACGCCAA GCGCTTGCAG TATCTCTTTG CCCAGCTGGC GGACTGCAAG 
AACGATACGG AGCAACGCAC TTGGATGCTC TACGAGGATG AGGAGGACAT CATTCAGTTC 
CTTGAGGAGC TTGTTGAGAT TTTGATCAAT GCTGATGAGA GCATCAGTTG CTACGAGATG 
TCCTGCGATC AGTACCAGAT GTTTATCAAT CTGGTGCAGT ACTATCAGAT GGAGACGCGC 
TGGTCCATCA AGCGACTGCT GCTTAAGACC TTTACGGCCG CTTGCCATCT GGACTATATC 
ATTGTGGACA TATTGCTGAC CTCGGTGCTG CCATTGGAGA TTGTCGAGGA CATGAAGACG 
CACTTCTCCA ATCTGGATCG CTTCAAGCAG CTGGTCAAGA TGCTCACTAT TATCTTCTCA 
CTTGGACAGC CCATGCCGGT TAATCATCAG GATTATTTGG GCGTACATTT TGCCAGCTTC 
CTACTGGAAA TCGTCGAGGG CAATAATCCG GAGGTCTTGG TGGACATGGT CATTGCCTTG 
ATCTTGGCCT TCAATCAGCA ATTTAGCGAA CATACCTATA ATGTCATCAT CGAAGGTATG 
CAGAATCTGC CATCCGCCAA AGTGTTTACG GAGAAGTTGC TACTTTTGCT CAATCGAGAG 
GATGATCCCA CACGCTTGCT CAAGCATCCC AACGAGCATA TGAACACGGT GCTGCGAATG 
TTTATCGACA TATTCAGCCA TCCGGATACG GCGGGCATGT TCTACACGAA TGACATCAAG 
GTGCTTATCG ATATAGTGGT TCGCCAGCTA TCCGATTTGG ATGCCGGCAG TACGACGCGA 
CCATGCTACT TGGAGCTTTG CCGACGCATC CTGCGCAATA CGAACTATCA GGAGCACCAG 
CATCGCAAGC ATGATCTCAT GAAGATCTTC ACGCGCATCT TCTGCGAGGA GACCGAGTGC 
AGTGCCTCCG ATCAGCAGCT GGTGCGGGAA ATAGCGAACG AATTTCCGCA GCTGTTCAAG 
GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572439.1
CDS	1120..3525
Translation	MLKALYDFQAVYPKTISFDEGEYFILYQTSARQRNWWQVVSMKGNIGFVPSNYVMKIKVEHDFLISFLNSSIESLEKCTDHEINGIMSKDELLDRLREKKHTMERLYAESSERDGDSSLSYSHSYSDKGSHRHSHPHPTQSQTQLHSQHRQHSPPPSGGSVGSQRLDMSKKSMSSPAVGSSCGLPQNQGMIESPSMGSMQFQGSSCTIQTPQQQQPLPAPPAPAPSPSAASATPTAASTSKAATGGDVAQDNSITSEPSETTTTTTTTSEDVVTTYKETSQMSTSQQHKPQNGSTASASISASASASALRSNGGNGKANGSATALHQELQQEEADSAPDKSDDASAVQSDDGCPANGDSADNSQALDSIDSPSHRQRGLSLGRIEEGATGGGQLKVESSDVYQIVDALRRNTNLSFDLSCEALRVVLTSLEQLYNGAINPYLEAVAVHVTGKVATPKELLGITHDAKRLQYLFAQLADCKNDTEQRTWMLYEDEEDIIQFLEELVEILINADESISCYEMSCDQYQMFINLVQYYQMETRWSIKRLLLKTFTAACHLDYIIVDILLTSVLPLEIVEDMKTHFSNLDRFKQLVKMLTIIFSLGQPMPVNHQDYLGVHFASFLLEIVEGNNPEVLVDMVIALILAFNQQFSEHTYNVIIEGMQNLPSAKVFTEKLLLLLNREDDPTRLLKHPNEHMNTVLRMFIDIFSHPDTAGMFYTNDIKVLIDIVVRQLSDLDAGSTTRPCYLELCRRILRNTNYQEHQHRKHDLMKIFTRIFCEETECSASDQQLVREIANEFPQLFKA

Target ORF information:

RefSeq Version	NM_132211.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG2258), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_132211.2

ORF Insert Sequence:

ATGCTCAAGG CTCTGTACGA CTTCCAGGCG GTCTATCCGA AGACCATCAG CTTCGATGAG 
GGCGAGTACT TCATCCTTTA CCAGACGTCC GCCCGCCAGC GCAATTGGTG GCAGGTTGTC 
AGCATGAAAG GCAACATTGG CTTTGTGCCC TCCAATTACG TAATGAAGAT TAAGGTGGAG 
CACGACTTTC TCATCAGCTT TCTGAACTCC TCGATAGAAT CGCTGGAGAA GTGCACGGAC 
CATGAGATCA ATGGCATCAT GTCCAAGGAC GAACTGCTGG ACAGGCTGCG CGAGAAGAAG 
CACACCATGG AGCGTCTGTA TGCGGAGAGT TCCGAGCGCG ACGGTGACTC CTCGCTTTCC 
TATTCACACA GCTACAGTGA CAAGGGTAGC CACCGGCACT CGCATCCCCA TCCCACCCAA 
TCCCAGACGC AGCTTCATTC CCAGCACAGG CAGCACAGTC CGCCGCCCAG CGGCGGCAGC 
GTTGGCTCCC AGCGTTTGGA CATGAGCAAG AAGAGCATGT CAAGTCCAGC GGTGGGCTCA 
TCGTGCGGCC TGCCCCAGAA TCAGGGCATG ATCGAATCGC CCAGCATGGG CAGCATGCAG 
TTCCAGGGCA GCAGCTGCAC CATCCAGACG CCGCAGCAGC AACAGCCGCT CCCAGCACCG 
CCAGCACCAG CTCCCTCGCC ATCGGCAGCA TCGGCCACAC CCACTGCGGC ATCAACGAGC 
AAAGCGGCCA CCGGCGGTGA TGTCGCGCAG GACAATAGCA TTACGTCGGA GCCCTCGGAG 
ACGACAACGA CCACTACGAC GACCAGCGAA GATGTGGTCA CCACATACAA GGAGACCAGC 
CAAATGAGCA CCAGCCAGCA GCATAAGCCG CAAAATGGGA GCACTGCCTC GGCGTCCATT 
TCCGCATCTG CCTCGGCCTC AGCTTTGCGC AGCAACGGCG GCAACGGCAA GGCCAACGGT 
AGTGCCACAG CCCTGCATCA GGAGCTGCAG CAGGAGGAAG CGGACAGTGC GCCGGACAAG 
TCGGATGATG CCAGCGCCGT GCAAAGTGAC GACGGTTGCC CGGCCAACGG AGATAGTGCG 
GATAATAGCC AGGCGCTGGA CAGCATCGAC AGTCCGTCGC ATCGACAGCG CGGCCTGAGC 
CTGGGCAGAA TCGAGGAGGG TGCCACAGGA GGAGGCCAGT TGAAGGTTGA GTCATCGGAT 
GTATATCAGA TTGTGGACGC CCTGCGGCGG AACACCAACC TCAGCTTCGA TCTCTCCTGT 
GAAGCATTGC GCGTGGTGCT GACCAGCCTG GAGCAGCTGT ACAACGGTGC CATCAATCCG 
TACCTGGAGG CGGTGGCCGT TCATGTCACT GGCAAAGTGG CCACGCCCAA GGAGCTGCTG 
GGCATTACGC ACGACGCCAA GCGCTTGCAG TATCTCTTTG CCCAGCTGGC GGACTGCAAG 
AACGATACGG AGCAACGCAC TTGGATGCTC TACGAGGATG AGGAGGACAT CATTCAGTTC 
CTTGAGGAGC TTGTTGAGAT TTTGATCAAT GCTGATGAGA GCATCAGTTG CTACGAGATG 
TCCTGCGATC AGTACCAGAT GTTTATCAAT CTGGTGCAGT ACTATCAGAT GGAGACGCGC 
TGGTCCATCA AGCGACTGCT GCTTAAGACC TTTACGGCCG CTTGCCATCT GGACTATATC 
ATTGTGGACA TATTGCTGAC CTCGGTGCTG CCATTGGAGA TTGTCGAGGA CATGAAGACG 
CACTTCTCCA ATCTGGATCG CTTCAAGCAG CTGGTCAAGA TGCTCACTAT TATCTTCTCA 
CTTGGACAGC CCATGCCGGT TAATCATCAG GATTATTTGG GCGTACATTT TGCCAGCTTC 
CTACTGGAAA TCGTCGAGGG CAATAATCCG GAGGTCTTGG TGGACATGGT CATTGCCTTG 
ATCTTGGCCT TCAATCAGCA ATTTAGCGAA CATACCTATA ATGTCATCAT CGAAGGTATG 
CAGAATCTGC CATCCGCCAA AGTGTTTACG GAGAAGTTGC TACTTTTGCT CAATCGAGAG 
GATGATCCCA CACGCTTGCT CAAGCATCCC AACGAGCATA TGAACACGGT GCTGCGAATG 
TTTATCGACA TATTCAGCCA TCCGGATACG GCGGGCATGT TCTACACGAA TGACATCAAG 
GTGCTTATCG ATATAGTGGT TCGCCAGCTA TCCGATTTGG ATGCCGGCAG TACGACGCGA 
CCATGCTACT TGGAGCTTTG CCGACGCATC CTGCGCAATA CGAACTATCA GGAGCACCAG 
CATCGCAAGC ATGATCTCAT GAAGATCTTC ACGCGCATCT TCTGCGAGGA GACCGAGTGC 
AGTGCCTCCG ATCAGCAGCT GGTGCGGGAA ATAGCGAACG AATTTCCGCA GCTGTTCAAG 
GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG2258 ( NM_001298071.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285000.1 Drosophila melanogaster uncharacterized protein, transcript variant D (CG2258), mRNA.

Price & Availability↑

CloneID	OFa01075
Clone ID Related Accession (Same CDS sequence)	NM_206649.2 , NM_206648.2 , NM_132211.2 , NM_001298071.1
Accession Version	NM_001298071.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCTCAAGG CTCTGTACGA CTTCCAGGCG GTCTATCCGA AGACCATCAG CTTCGATGAG 
GGCGAGTACT TCATCCTTTA CCAGACGTCC GCCCGCCAGC GCAATTGGTG GCAGGTTGTC 
AGCATGAAAG GCAACATTGG CTTTGTGCCC TCCAATTACG TAATGAAGAT TAAGGTGGAG 
CACGACTTTC TCATCAGCTT TCTGAACTCC TCGATAGAAT CGCTGGAGAA GTGCACGGAC 
CATGAGATCA ATGGCATCAT GTCCAAGGAC GAACTGCTGG ACAGGCTGCG CGAGAAGAAG 
CACACCATGG AGCGTCTGTA TGCGGAGAGT TCCGAGCGCG ACGGTGACTC CTCGCTTTCC 
TATTCACACA GCTACAGTGA CAAGGGTAGC CACCGGCACT CGCATCCCCA TCCCACCCAA 
TCCCAGACGC AGCTTCATTC CCAGCACAGG CAGCACAGTC CGCCGCCCAG CGGCGGCAGC 
GTTGGCTCCC AGCGTTTGGA CATGAGCAAG AAGAGCATGT CAAGTCCAGC GGTGGGCTCA 
TCGTGCGGCC TGCCCCAGAA TCAGGGCATG ATCGAATCGC CCAGCATGGG CAGCATGCAG 
TTCCAGGGCA GCAGCTGCAC CATCCAGACG CCGCAGCAGC AACAGCCGCT CCCAGCACCG 
CCAGCACCAG CTCCCTCGCC ATCGGCAGCA TCGGCCACAC CCACTGCGGC ATCAACGAGC 
AAAGCGGCCA CCGGCGGTGA TGTCGCGCAG GACAATAGCA TTACGTCGGA GCCCTCGGAG 
ACGACAACGA CCACTACGAC GACCAGCGAA GATGTGGTCA CCACATACAA GGAGACCAGC 
CAAATGAGCA CCAGCCAGCA GCATAAGCCG CAAAATGGGA GCACTGCCTC GGCGTCCATT 
TCCGCATCTG CCTCGGCCTC AGCTTTGCGC AGCAACGGCG GCAACGGCAA GGCCAACGGT 
AGTGCCACAG CCCTGCATCA GGAGCTGCAG CAGGAGGAAG CGGACAGTGC GCCGGACAAG 
TCGGATGATG CCAGCGCCGT GCAAAGTGAC GACGGTTGCC CGGCCAACGG AGATAGTGCG 
GATAATAGCC AGGCGCTGGA CAGCATCGAC AGTCCGTCGC ATCGACAGCG CGGCCTGAGC 
CTGGGCAGAA TCGAGGAGGG TGCCACAGGA GGAGGCCAGT TGAAGGTTGA GTCATCGGAT 
GTATATCAGA TTGTGGACGC CCTGCGGCGG AACACCAACC TCAGCTTCGA TCTCTCCTGT 
GAAGCATTGC GCGTGGTGCT GACCAGCCTG GAGCAGCTGT ACAACGGTGC CATCAATCCG 
TACCTGGAGG CGGTGGCCGT TCATGTCACT GGCAAAGTGG CCACGCCCAA GGAGCTGCTG 
GGCATTACGC ACGACGCCAA GCGCTTGCAG TATCTCTTTG CCCAGCTGGC GGACTGCAAG 
AACGATACGG AGCAACGCAC TTGGATGCTC TACGAGGATG AGGAGGACAT CATTCAGTTC 
CTTGAGGAGC TTGTTGAGAT TTTGATCAAT GCTGATGAGA GCATCAGTTG CTACGAGATG 
TCCTGCGATC AGTACCAGAT GTTTATCAAT CTGGTGCAGT ACTATCAGAT GGAGACGCGC 
TGGTCCATCA AGCGACTGCT GCTTAAGACC TTTACGGCCG CTTGCCATCT GGACTATATC 
ATTGTGGACA TATTGCTGAC CTCGGTGCTG CCATTGGAGA TTGTCGAGGA CATGAAGACG 
CACTTCTCCA ATCTGGATCG CTTCAAGCAG CTGGTCAAGA TGCTCACTAT TATCTTCTCA 
CTTGGACAGC CCATGCCGGT TAATCATCAG GATTATTTGG GCGTACATTT TGCCAGCTTC 
CTACTGGAAA TCGTCGAGGG CAATAATCCG GAGGTCTTGG TGGACATGGT CATTGCCTTG 
ATCTTGGCCT TCAATCAGCA ATTTAGCGAA CATACCTATA ATGTCATCAT CGAAGGTATG 
CAGAATCTGC CATCCGCCAA AGTGTTTACG GAGAAGTTGC TACTTTTGCT CAATCGAGAG 
GATGATCCCA CACGCTTGCT CAAGCATCCC AACGAGCATA TGAACACGGT GCTGCGAATG 
TTTATCGACA TATTCAGCCA TCCGGATACG GCGGGCATGT TCTACACGAA TGACATCAAG 
GTGCTTATCG ATATAGTGGT TCGCCAGCTA TCCGATTTGG ATGCCGGCAG TACGACGCGA 
CCATGCTACT TGGAGCTTTG CCGACGCATC CTGCGCAATA CGAACTATCA GGAGCACCAG 
CATCGCAAGC ATGATCTCAT GAAGATCTTC ACGCGCATCT TCTGCGAGGA GACCGAGTGC 
AGTGCCTCCG ATCAGCAGCT GGTGCGGGAA ATAGCGAACG AATTTCCGCA GCTGTTCAAG 
GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285000.1
CDS	479..2884
Translation	MLKALYDFQAVYPKTISFDEGEYFILYQTSARQRNWWQVVSMKGNIGFVPSNYVMKIKVEHDFLISFLNSSIESLEKCTDHEINGIMSKDELLDRLREKKHTMERLYAESSERDGDSSLSYSHSYSDKGSHRHSHPHPTQSQTQLHSQHRQHSPPPSGGSVGSQRLDMSKKSMSSPAVGSSCGLPQNQGMIESPSMGSMQFQGSSCTIQTPQQQQPLPAPPAPAPSPSAASATPTAASTSKAATGGDVAQDNSITSEPSETTTTTTTTSEDVVTTYKETSQMSTSQQHKPQNGSTASASISASASASALRSNGGNGKANGSATALHQELQQEEADSAPDKSDDASAVQSDDGCPANGDSADNSQALDSIDSPSHRQRGLSLGRIEEGATGGGQLKVESSDVYQIVDALRRNTNLSFDLSCEALRVVLTSLEQLYNGAINPYLEAVAVHVTGKVATPKELLGITHDAKRLQYLFAQLADCKNDTEQRTWMLYEDEEDIIQFLEELVEILINADESISCYEMSCDQYQMFINLVQYYQMETRWSIKRLLLKTFTAACHLDYIIVDILLTSVLPLEIVEDMKTHFSNLDRFKQLVKMLTIIFSLGQPMPVNHQDYLGVHFASFLLEIVEGNNPEVLVDMVIALILAFNQQFSEHTYNVIIEGMQNLPSAKVFTEKLLLLLNREDDPTRLLKHPNEHMNTVLRMFIDIFSHPDTAGMFYTNDIKVLIDIVVRQLSDLDAGSTTRPCYLELCRRILRNTNYQEHQHRKHDLMKIFTRIFCEETECSASDQQLVREIANEFPQLFKA

Target ORF information:

RefSeq Version	NM_001298071.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant D (CG2258), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298071.1

ORF Insert Sequence:

ATGCTCAAGG CTCTGTACGA CTTCCAGGCG GTCTATCCGA AGACCATCAG CTTCGATGAG 
GGCGAGTACT TCATCCTTTA CCAGACGTCC GCCCGCCAGC GCAATTGGTG GCAGGTTGTC 
AGCATGAAAG GCAACATTGG CTTTGTGCCC TCCAATTACG TAATGAAGAT TAAGGTGGAG 
CACGACTTTC TCATCAGCTT TCTGAACTCC TCGATAGAAT CGCTGGAGAA GTGCACGGAC 
CATGAGATCA ATGGCATCAT GTCCAAGGAC GAACTGCTGG ACAGGCTGCG CGAGAAGAAG 
CACACCATGG AGCGTCTGTA TGCGGAGAGT TCCGAGCGCG ACGGTGACTC CTCGCTTTCC 
TATTCACACA GCTACAGTGA CAAGGGTAGC CACCGGCACT CGCATCCCCA TCCCACCCAA 
TCCCAGACGC AGCTTCATTC CCAGCACAGG CAGCACAGTC CGCCGCCCAG CGGCGGCAGC 
GTTGGCTCCC AGCGTTTGGA CATGAGCAAG AAGAGCATGT CAAGTCCAGC GGTGGGCTCA 
TCGTGCGGCC TGCCCCAGAA TCAGGGCATG ATCGAATCGC CCAGCATGGG CAGCATGCAG 
TTCCAGGGCA GCAGCTGCAC CATCCAGACG CCGCAGCAGC AACAGCCGCT CCCAGCACCG 
CCAGCACCAG CTCCCTCGCC ATCGGCAGCA TCGGCCACAC CCACTGCGGC ATCAACGAGC 
AAAGCGGCCA CCGGCGGTGA TGTCGCGCAG GACAATAGCA TTACGTCGGA GCCCTCGGAG 
ACGACAACGA CCACTACGAC GACCAGCGAA GATGTGGTCA CCACATACAA GGAGACCAGC 
CAAATGAGCA CCAGCCAGCA GCATAAGCCG CAAAATGGGA GCACTGCCTC GGCGTCCATT 
TCCGCATCTG CCTCGGCCTC AGCTTTGCGC AGCAACGGCG GCAACGGCAA GGCCAACGGT 
AGTGCCACAG CCCTGCATCA GGAGCTGCAG CAGGAGGAAG CGGACAGTGC GCCGGACAAG 
TCGGATGATG CCAGCGCCGT GCAAAGTGAC GACGGTTGCC CGGCCAACGG AGATAGTGCG 
GATAATAGCC AGGCGCTGGA CAGCATCGAC AGTCCGTCGC ATCGACAGCG CGGCCTGAGC 
CTGGGCAGAA TCGAGGAGGG TGCCACAGGA GGAGGCCAGT TGAAGGTTGA GTCATCGGAT 
GTATATCAGA TTGTGGACGC CCTGCGGCGG AACACCAACC TCAGCTTCGA TCTCTCCTGT 
GAAGCATTGC GCGTGGTGCT GACCAGCCTG GAGCAGCTGT ACAACGGTGC CATCAATCCG 
TACCTGGAGG CGGTGGCCGT TCATGTCACT GGCAAAGTGG CCACGCCCAA GGAGCTGCTG 
GGCATTACGC ACGACGCCAA GCGCTTGCAG TATCTCTTTG CCCAGCTGGC GGACTGCAAG 
AACGATACGG AGCAACGCAC TTGGATGCTC TACGAGGATG AGGAGGACAT CATTCAGTTC 
CTTGAGGAGC TTGTTGAGAT TTTGATCAAT GCTGATGAGA GCATCAGTTG CTACGAGATG 
TCCTGCGATC AGTACCAGAT GTTTATCAAT CTGGTGCAGT ACTATCAGAT GGAGACGCGC 
TGGTCCATCA AGCGACTGCT GCTTAAGACC TTTACGGCCG CTTGCCATCT GGACTATATC 
ATTGTGGACA TATTGCTGAC CTCGGTGCTG CCATTGGAGA TTGTCGAGGA CATGAAGACG 
CACTTCTCCA ATCTGGATCG CTTCAAGCAG CTGGTCAAGA TGCTCACTAT TATCTTCTCA 
CTTGGACAGC CCATGCCGGT TAATCATCAG GATTATTTGG GCGTACATTT TGCCAGCTTC 
CTACTGGAAA TCGTCGAGGG CAATAATCCG GAGGTCTTGG TGGACATGGT CATTGCCTTG 
ATCTTGGCCT TCAATCAGCA ATTTAGCGAA CATACCTATA ATGTCATCAT CGAAGGTATG 
CAGAATCTGC CATCCGCCAA AGTGTTTACG GAGAAGTTGC TACTTTTGCT CAATCGAGAG 
GATGATCCCA CACGCTTGCT CAAGCATCCC AACGAGCATA TGAACACGGT GCTGCGAATG 
TTTATCGACA TATTCAGCCA TCCGGATACG GCGGGCATGT TCTACACGAA TGACATCAAG 
GTGCTTATCG ATATAGTGGT TCGCCAGCTA TCCGATTTGG ATGCCGGCAG TACGACGCGA 
CCATGCTACT TGGAGCTTTG CCGACGCATC CTGCGCAATA CGAACTATCA GGAGCACCAG 
CATCGCAAGC ATGATCTCAT GAAGATCTTC ACGCGCATCT TCTGCGAGGA GACCGAGTGC 
AGTGCCTCCG ATCAGCAGCT GGTGCGGGAA ATAGCGAACG AATTTCCGCA GCTGTTCAAG 
GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG2258 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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