fz4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	fz4
Entrez Gene ID	31659
Full Name	frizzled 4
Synonyms	CG4626,DFz4,Dfz4,Dm Fz4,Dmel\CG4626,Fz4,anon-WO0170980.10,anon-WO0170980.11
General protein information	Preferred Names frizzled 4 Names CG4626 gene product from transcript CG4626-RB CG4626-PA CG4626-PB Frizzled4 fz4-PA fz4-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 6F5-6F5

mRNA and Protein(s)

mRNA	Protein	Name
NM_167119.3	NP_727170.2	frizzled 4, isoform B
NM_078513.3	NP_511068.2	frizzled 4, isoform A

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	fz4	NP_511068.2
Anopheles gambiae (African malaria mosquito)	GPRFZ4	XP_319612.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Knockdown of Tc-fz1 alone interfered with the formation of the proximo-distal and the dorso-ventral axes during leg development, whereas no effect was observed with single Tc-fz2 or Tc-fz4 RNAi knockdowns.
Title: A context-dependent combination of Wnt receptors controls axis elongation and leg development in a short germ insect.

The following fz4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fz4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa01004	NM_167119.3 Latest version!	Drosophila melanogaster frizzled 4 (fz4), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa01004	NM_078513.3 Latest version!	Drosophila melanogaster frizzled 4 (fz4), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

fz4 ( NM_167119.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727170.2 Drosophila melanogaster frizzled 4 (fz4), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa01004
Clone ID Related Accession (Same CDS sequence)	NM_167119.3 , NM_078513.3
Accession Version	NM_167119.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	frizzled 4, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167119.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGCCAA CCTGCATATT GTGTCTCCTG GTGGTGATCC TCCTGCATCC GCGCATCTCA 
AAATCCTCAA CGAGCGGCAA CCCCAGTGCC AGTTCCAGTT CCAGTTCTCC GCCAGAAATC 
CCCGCCTTCC GGCAATGCGA AACCATTCGC ATTGAGATGT GCCGCAAGAT CGGCTACAAT 
GAGACCTCCA TGCCAAATCT GGTGGGTAAC GAAATGCAAA CGGACGTGGA GTACACACTG 
CAAACCTTTG CACCACTGAT CGAATACGAC TGCAGTTCCC AGTTGAAGCT GTTCCTGTGC 
GCCGCCTACG TGCCCATGTG CACGCCCAAA GCTCCAGTCC ATGCCATCGG TCCGTGCCGG 
AGTCTCTGTG AGTCCGTGCG AATACGTTGT CATCCAGTTT TGCAGGGATT CGGATTTCCA 
TGGCCACCGG CTCTCGACTG CGATAAATTT CCGCGAGAGA ACAACCACGA GACGATGTGT 
ATGGAAGGTC CCGGTGAATT GCATCAGCCG CAGCAGGAGC AGGATTTGTA TGGGCTACCG 
GGCCAAGGAA TACCCGGTGG ATTGGGCGGT AAACTGCCCA TGGATTGCTC GGGACTGGCC 
AAATCGCATC TGTACGTAAG GCTACCCAGA TCTGGACGCT GTGCACCACT CTGCGAGGCG 
GACATACTGT TCACACCGGC GGAGAAGCAT CTGGCAGAGA TCTGGGTCTC CACATGGGCC 
TATGCCGCTT TGGGTTTGGC CCTGGTAGCC ACCGTGTGTC TTTTGGCCAG CGATGGCAGT 
CGTTTGGCCA GCGCCAAGTG GTCCAGATTG TTGTCGCCGC TGATTTGGTG CCACAACATG 
GTCACCTTGG GCTGGGCAGT GCGCTTTATG GTGGGAAGAA CGGGAACTGC GTGCGGCACG 
GATCCCCAGG CGCCCAATGA ATCCCTACTC ACCGTGGACG GACTGTCCAA TGCCTCCTGC 
GCTAGTGTCT TCCTTATGCG CTACTATTTT GGAATGGCCG CCTGCGCTTG GTGGGCTGTG 
CTCTGTTTGG GCTGGCACCG CGACATTCGT CGGCACAGTC CGGACTCCAA GGGCCATGTG 
GTCATTCCCT CGAACTTTGG AGGCAGTCCG GCGAAGAGGA ACAGTGCCAA GACGGCTCAG 
CAGGATCTAA CGCAAAACAA CTTTGTGTGC TTCGTGGCCT GGGGATTGCC GGCCTTTCAA 
ACGTCAGCAG TGATCGTTGC CCGTTTCGTG GATGCTGACG AGTTGCTTGG CGCCTGTTTC 
GTAGGCAATC AGTCGGATAA GGCGCTACAG ATATTGGTGG CTACGCCCGT TTTTTGCTAC 
TGGATCTTTG GCTCGATGAA TCTGATCTCC GGCTATTTGG TACACTGTCG CACCAAGGAG 
ATCCTGAGGA ATAGCAATGC ACTCAGCGTG CAACAGCAAC TGCAACAGCT AAGCGCACAC 
AGTAGTTCCG GCATTGGCAT ATTCCTTTTC ATCTATGGAC TTGCATGTGC CATGCTTCTG 
TTGGCTGTCA TTTACGAGTT TGCCAACATC GATGTCTGGC TGGGATCGGG TGATACCAAT 
ACGCCACTTT GGCCATTTTT GTTGCGCGCC TTCATGGAAC TGATGCTGGG CATTTGCTGT 
TTTGCCTGGG TACTTGGACC AAGCATTTCC ACTCTCTACA AGAGGCAAGT CAGCAATGGC 
AAAATGGTGA AGCATACGTC TGCCGGAGCA GCAACTGGAC ACCTGGACGG GCATAGTAGC 
TCGCGTGGAT CGCATGCGGC TTGCAATAGC ACGGTGGTCT CCTACCATTC GGTGCGAACT 
TCTATGGCCA GTGTTCCGTT GCCGCCAAGT CCGTACAAAC TGAAGACTTC GCCTGGTACC 
GGCTCTATAT CGCTGAATCA GATGTCCAAC TATTCGCTGG GCAGGAGCGT GCATCACCAG 
CAGCGCCATT CGCCGCATCA TCATCATCAC CAGCAGCAGC AGCATCATCA GTTCCATCCC 
CACCACAATC ACCAGCATCA CTCCACATCC TCGCATCGGC TGTATTATCC GCCAGGTAGC 
TATGCCTCGC AAAAGTACAG CCAGCACGGC AGCTACTATC CCCACTTGCA GCAGTATGGC 
AATGAGACGC TACTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727170.2
CDS	448..2565
Translation	MKPTCILCLLVVILLHPRISKSSTSGNPSASSSSSSPPEIPAFRQCETIRIEMCRKIGYNETSMPNLVGNEMQTDVEYTLQTFAPLIEYDCSSQLKLFLCAAYVPMCTPKAPVHAIGPCRSLCESVRIRCHPVLQGFGFPWPPALDCDKFPRENNHETMCMEGPGELHQPQQEQDLYGLPGQGIPGGLGGKLPMDCSGLAKSHLYVRLPRSGRCAPLCEADILFTPAEKHLAEIWVSTWAYAALGLALVATVCLLASDGSRLASAKWSRLLSPLIWCHNMVTLGWAVRFMVGRTGTACGTDPQAPNESLLTVDGLSNASCASVFLMRYYFGMAACAWWAVLCLGWHRDIRRHSPDSKGHVVIPSNFGGSPAKRNSAKTAQQDLTQNNFVCFVAWGLPAFQTSAVIVARFVDADELLGACFVGNQSDKALQILVATPVFCYWIFGSMNLISGYLVHCRTKEILRNSNALSVQQQLQQLSAHSSSGIGIFLFIYGLACAMLLLAVIYEFANIDVWLGSGDTNTPLWPFLLRAFMELMLGICCFAWVLGPSISTLYKRQVSNGKMVKHTSAGAATGHLDGHSSSRGSHAACNSTVVSYHSVRTSMASVPLPPSPYKLKTSPGTGSISLNQMSNYSLGRSVHHQQRHSPHHHHHQQQQHHQFHPHHNHQHHSTSSHRLYYPPGSYASQKYSQHGSYYPHLQQYGNETLL

Target ORF information:

RefSeq Version	NM_167119.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster frizzled 4 (fz4), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167119.3

ORF Insert Sequence:

ATGAAGCCAA CCTGCATATT GTGTCTCCTG GTGGTGATCC TCCTGCATCC GCGCATCTCA 
AAATCCTCAA CGAGCGGCAA CCCCAGTGCC AGTTCCAGTT CCAGTTCTCC GCCAGAAATC 
CCCGCCTTCC GGCAATGCGA AACCATTCGC ATTGAGATGT GCCGCAAGAT CGGCTACAAT 
GAGACCTCCA TGCCAAATCT GGTGGGTAAC GAAATGCAAA CGGACGTGGA GTACACACTG 
CAAACCTTTG CACCACTGAT CGAATACGAC TGCAGTTCCC AGTTGAAGCT GTTCCTGTGC 
GCCGCCTACG TGCCCATGTG CACGCCCAAA GCTCCAGTCC ATGCCATCGG TCCGTGCCGG 
AGTCTCTGTG AGTCCGTGCG AATACGTTGT CATCCAGTTT TGCAGGGATT CGGATTTCCA 
TGGCCACCGG CTCTCGACTG CGATAAATTT CCGCGAGAGA ACAACCACGA GACGATGTGT 
ATGGAAGGTC CCGGTGAATT GCATCAGCCG CAGCAGGAGC AGGATTTGTA TGGGCTACCG 
GGCCAAGGAA TACCCGGTGG ATTGGGCGGT AAACTGCCCA TGGATTGCTC GGGACTGGCC 
AAATCGCATC TGTACGTAAG GCTACCCAGA TCTGGACGCT GTGCACCACT CTGCGAGGCG 
GACATACTGT TCACACCGGC GGAGAAGCAT CTGGCAGAGA TCTGGGTCTC CACATGGGCC 
TATGCCGCTT TGGGTTTGGC CCTGGTAGCC ACCGTGTGTC TTTTGGCCAG CGATGGCAGT 
CGTTTGGCCA GCGCCAAGTG GTCCAGATTG TTGTCGCCGC TGATTTGGTG CCACAACATG 
GTCACCTTGG GCTGGGCAGT GCGCTTTATG GTGGGAAGAA CGGGAACTGC GTGCGGCACG 
GATCCCCAGG CGCCCAATGA ATCCCTACTC ACCGTGGACG GACTGTCCAA TGCCTCCTGC 
GCTAGTGTCT TCCTTATGCG CTACTATTTT GGAATGGCCG CCTGCGCTTG GTGGGCTGTG 
CTCTGTTTGG GCTGGCACCG CGACATTCGT CGGCACAGTC CGGACTCCAA GGGCCATGTG 
GTCATTCCCT CGAACTTTGG AGGCAGTCCG GCGAAGAGGA ACAGTGCCAA GACGGCTCAG 
CAGGATCTAA CGCAAAACAA CTTTGTGTGC TTCGTGGCCT GGGGATTGCC GGCCTTTCAA 
ACGTCAGCAG TGATCGTTGC CCGTTTCGTG GATGCTGACG AGTTGCTTGG CGCCTGTTTC 
GTAGGCAATC AGTCGGATAA GGCGCTACAG ATATTGGTGG CTACGCCCGT TTTTTGCTAC 
TGGATCTTTG GCTCGATGAA TCTGATCTCC GGCTATTTGG TACACTGTCG CACCAAGGAG 
ATCCTGAGGA ATAGCAATGC ACTCAGCGTG CAACAGCAAC TGCAACAGCT AAGCGCACAC 
AGTAGTTCCG GCATTGGCAT ATTCCTTTTC ATCTATGGAC TTGCATGTGC CATGCTTCTG 
TTGGCTGTCA TTTACGAGTT TGCCAACATC GATGTCTGGC TGGGATCGGG TGATACCAAT 
ACGCCACTTT GGCCATTTTT GTTGCGCGCC TTCATGGAAC TGATGCTGGG CATTTGCTGT 
TTTGCCTGGG TACTTGGACC AAGCATTTCC ACTCTCTACA AGAGGCAAGT CAGCAATGGC 
AAAATGGTGA AGCATACGTC TGCCGGAGCA GCAACTGGAC ACCTGGACGG GCATAGTAGC 
TCGCGTGGAT CGCATGCGGC TTGCAATAGC ACGGTGGTCT CCTACCATTC GGTGCGAACT 
TCTATGGCCA GTGTTCCGTT GCCGCCAAGT CCGTACAAAC TGAAGACTTC GCCTGGTACC 
GGCTCTATAT CGCTGAATCA GATGTCCAAC TATTCGCTGG GCAGGAGCGT GCATCACCAG 
CAGCGCCATT CGCCGCATCA TCATCATCAC CAGCAGCAGC AGCATCATCA GTTCCATCCC 
CACCACAATC ACCAGCATCA CTCCACATCC TCGCATCGGC TGTATTATCC GCCAGGTAGC 
TATGCCTCGC AAAAGTACAG CCAGCACGGC AGCTACTATC CCCACTTGCA GCAGTATGGC 
AATGAGACGC TACTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fz4 ( NM_078513.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_511068.2 Drosophila melanogaster frizzled 4 (fz4), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa01004
Clone ID Related Accession (Same CDS sequence)	NM_167119.3 , NM_078513.3
Accession Version	NM_078513.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	frizzled 4, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_078513.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGCCAA CCTGCATATT GTGTCTCCTG GTGGTGATCC TCCTGCATCC GCGCATCTCA 
AAATCCTCAA CGAGCGGCAA CCCCAGTGCC AGTTCCAGTT CCAGTTCTCC GCCAGAAATC 
CCCGCCTTCC GGCAATGCGA AACCATTCGC ATTGAGATGT GCCGCAAGAT CGGCTACAAT 
GAGACCTCCA TGCCAAATCT GGTGGGTAAC GAAATGCAAA CGGACGTGGA GTACACACTG 
CAAACCTTTG CACCACTGAT CGAATACGAC TGCAGTTCCC AGTTGAAGCT GTTCCTGTGC 
GCCGCCTACG TGCCCATGTG CACGCCCAAA GCTCCAGTCC ATGCCATCGG TCCGTGCCGG 
AGTCTCTGTG AGTCCGTGCG AATACGTTGT CATCCAGTTT TGCAGGGATT CGGATTTCCA 
TGGCCACCGG CTCTCGACTG CGATAAATTT CCGCGAGAGA ACAACCACGA GACGATGTGT 
ATGGAAGGTC CCGGTGAATT GCATCAGCCG CAGCAGGAGC AGGATTTGTA TGGGCTACCG 
GGCCAAGGAA TACCCGGTGG ATTGGGCGGT AAACTGCCCA TGGATTGCTC GGGACTGGCC 
AAATCGCATC TGTACGTAAG GCTACCCAGA TCTGGACGCT GTGCACCACT CTGCGAGGCG 
GACATACTGT TCACACCGGC GGAGAAGCAT CTGGCAGAGA TCTGGGTCTC CACATGGGCC 
TATGCCGCTT TGGGTTTGGC CCTGGTAGCC ACCGTGTGTC TTTTGGCCAG CGATGGCAGT 
CGTTTGGCCA GCGCCAAGTG GTCCAGATTG TTGTCGCCGC TGATTTGGTG CCACAACATG 
GTCACCTTGG GCTGGGCAGT GCGCTTTATG GTGGGAAGAA CGGGAACTGC GTGCGGCACG 
GATCCCCAGG CGCCCAATGA ATCCCTACTC ACCGTGGACG GACTGTCCAA TGCCTCCTGC 
GCTAGTGTCT TCCTTATGCG CTACTATTTT GGAATGGCCG CCTGCGCTTG GTGGGCTGTG 
CTCTGTTTGG GCTGGCACCG CGACATTCGT CGGCACAGTC CGGACTCCAA GGGCCATGTG 
GTCATTCCCT CGAACTTTGG AGGCAGTCCG GCGAAGAGGA ACAGTGCCAA GACGGCTCAG 
CAGGATCTAA CGCAAAACAA CTTTGTGTGC TTCGTGGCCT GGGGATTGCC GGCCTTTCAA 
ACGTCAGCAG TGATCGTTGC CCGTTTCGTG GATGCTGACG AGTTGCTTGG CGCCTGTTTC 
GTAGGCAATC AGTCGGATAA GGCGCTACAG ATATTGGTGG CTACGCCCGT TTTTTGCTAC 
TGGATCTTTG GCTCGATGAA TCTGATCTCC GGCTATTTGG TACACTGTCG CACCAAGGAG 
ATCCTGAGGA ATAGCAATGC ACTCAGCGTG CAACAGCAAC TGCAACAGCT AAGCGCACAC 
AGTAGTTCCG GCATTGGCAT ATTCCTTTTC ATCTATGGAC TTGCATGTGC CATGCTTCTG 
TTGGCTGTCA TTTACGAGTT TGCCAACATC GATGTCTGGC TGGGATCGGG TGATACCAAT 
ACGCCACTTT GGCCATTTTT GTTGCGCGCC TTCATGGAAC TGATGCTGGG CATTTGCTGT 
TTTGCCTGGG TACTTGGACC AAGCATTTCC ACTCTCTACA AGAGGCAAGT CAGCAATGGC 
AAAATGGTGA AGCATACGTC TGCCGGAGCA GCAACTGGAC ACCTGGACGG GCATAGTAGC 
TCGCGTGGAT CGCATGCGGC TTGCAATAGC ACGGTGGTCT CCTACCATTC GGTGCGAACT 
TCTATGGCCA GTGTTCCGTT GCCGCCAAGT CCGTACAAAC TGAAGACTTC GCCTGGTACC 
GGCTCTATAT CGCTGAATCA GATGTCCAAC TATTCGCTGG GCAGGAGCGT GCATCACCAG 
CAGCGCCATT CGCCGCATCA TCATCATCAC CAGCAGCAGC AGCATCATCA GTTCCATCCC 
CACCACAATC ACCAGCATCA CTCCACATCC TCGCATCGGC TGTATTATCC GCCAGGTAGC 
TATGCCTCGC AAAAGTACAG CCAGCACGGC AGCTACTATC CCCACTTGCA GCAGTATGGC 
AATGAGACGC TACTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_511068.2
CDS	701..2818
Translation	MKPTCILCLLVVILLHPRISKSSTSGNPSASSSSSSPPEIPAFRQCETIRIEMCRKIGYNETSMPNLVGNEMQTDVEYTLQTFAPLIEYDCSSQLKLFLCAAYVPMCTPKAPVHAIGPCRSLCESVRIRCHPVLQGFGFPWPPALDCDKFPRENNHETMCMEGPGELHQPQQEQDLYGLPGQGIPGGLGGKLPMDCSGLAKSHLYVRLPRSGRCAPLCEADILFTPAEKHLAEIWVSTWAYAALGLALVATVCLLASDGSRLASAKWSRLLSPLIWCHNMVTLGWAVRFMVGRTGTACGTDPQAPNESLLTVDGLSNASCASVFLMRYYFGMAACAWWAVLCLGWHRDIRRHSPDSKGHVVIPSNFGGSPAKRNSAKTAQQDLTQNNFVCFVAWGLPAFQTSAVIVARFVDADELLGACFVGNQSDKALQILVATPVFCYWIFGSMNLISGYLVHCRTKEILRNSNALSVQQQLQQLSAHSSSGIGIFLFIYGLACAMLLLAVIYEFANIDVWLGSGDTNTPLWPFLLRAFMELMLGICCFAWVLGPSISTLYKRQVSNGKMVKHTSAGAATGHLDGHSSSRGSHAACNSTVVSYHSVRTSMASVPLPPSPYKLKTSPGTGSISLNQMSNYSLGRSVHHQQRHSPHHHHHQQQQHHQFHPHHNHQHHSTSSHRLYYPPGSYASQKYSQHGSYYPHLQQYGNETLL

Target ORF information:

RefSeq Version	NM_078513.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster frizzled 4 (fz4), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078513.3

ORF Insert Sequence:

ATGAAGCCAA CCTGCATATT GTGTCTCCTG GTGGTGATCC TCCTGCATCC GCGCATCTCA 
AAATCCTCAA CGAGCGGCAA CCCCAGTGCC AGTTCCAGTT CCAGTTCTCC GCCAGAAATC 
CCCGCCTTCC GGCAATGCGA AACCATTCGC ATTGAGATGT GCCGCAAGAT CGGCTACAAT 
GAGACCTCCA TGCCAAATCT GGTGGGTAAC GAAATGCAAA CGGACGTGGA GTACACACTG 
CAAACCTTTG CACCACTGAT CGAATACGAC TGCAGTTCCC AGTTGAAGCT GTTCCTGTGC 
GCCGCCTACG TGCCCATGTG CACGCCCAAA GCTCCAGTCC ATGCCATCGG TCCGTGCCGG 
AGTCTCTGTG AGTCCGTGCG AATACGTTGT CATCCAGTTT TGCAGGGATT CGGATTTCCA 
TGGCCACCGG CTCTCGACTG CGATAAATTT CCGCGAGAGA ACAACCACGA GACGATGTGT 
ATGGAAGGTC CCGGTGAATT GCATCAGCCG CAGCAGGAGC AGGATTTGTA TGGGCTACCG 
GGCCAAGGAA TACCCGGTGG ATTGGGCGGT AAACTGCCCA TGGATTGCTC GGGACTGGCC 
AAATCGCATC TGTACGTAAG GCTACCCAGA TCTGGACGCT GTGCACCACT CTGCGAGGCG 
GACATACTGT TCACACCGGC GGAGAAGCAT CTGGCAGAGA TCTGGGTCTC CACATGGGCC 
TATGCCGCTT TGGGTTTGGC CCTGGTAGCC ACCGTGTGTC TTTTGGCCAG CGATGGCAGT 
CGTTTGGCCA GCGCCAAGTG GTCCAGATTG TTGTCGCCGC TGATTTGGTG CCACAACATG 
GTCACCTTGG GCTGGGCAGT GCGCTTTATG GTGGGAAGAA CGGGAACTGC GTGCGGCACG 
GATCCCCAGG CGCCCAATGA ATCCCTACTC ACCGTGGACG GACTGTCCAA TGCCTCCTGC 
GCTAGTGTCT TCCTTATGCG CTACTATTTT GGAATGGCCG CCTGCGCTTG GTGGGCTGTG 
CTCTGTTTGG GCTGGCACCG CGACATTCGT CGGCACAGTC CGGACTCCAA GGGCCATGTG 
GTCATTCCCT CGAACTTTGG AGGCAGTCCG GCGAAGAGGA ACAGTGCCAA GACGGCTCAG 
CAGGATCTAA CGCAAAACAA CTTTGTGTGC TTCGTGGCCT GGGGATTGCC GGCCTTTCAA 
ACGTCAGCAG TGATCGTTGC CCGTTTCGTG GATGCTGACG AGTTGCTTGG CGCCTGTTTC 
GTAGGCAATC AGTCGGATAA GGCGCTACAG ATATTGGTGG CTACGCCCGT TTTTTGCTAC 
TGGATCTTTG GCTCGATGAA TCTGATCTCC GGCTATTTGG TACACTGTCG CACCAAGGAG 
ATCCTGAGGA ATAGCAATGC ACTCAGCGTG CAACAGCAAC TGCAACAGCT AAGCGCACAC 
AGTAGTTCCG GCATTGGCAT ATTCCTTTTC ATCTATGGAC TTGCATGTGC CATGCTTCTG 
TTGGCTGTCA TTTACGAGTT TGCCAACATC GATGTCTGGC TGGGATCGGG TGATACCAAT 
ACGCCACTTT GGCCATTTTT GTTGCGCGCC TTCATGGAAC TGATGCTGGG CATTTGCTGT 
TTTGCCTGGG TACTTGGACC AAGCATTTCC ACTCTCTACA AGAGGCAAGT CAGCAATGGC 
AAAATGGTGA AGCATACGTC TGCCGGAGCA GCAACTGGAC ACCTGGACGG GCATAGTAGC 
TCGCGTGGAT CGCATGCGGC TTGCAATAGC ACGGTGGTCT CCTACCATTC GGTGCGAACT 
TCTATGGCCA GTGTTCCGTT GCCGCCAAGT CCGTACAAAC TGAAGACTTC GCCTGGTACC 
GGCTCTATAT CGCTGAATCA GATGTCCAAC TATTCGCTGG GCAGGAGCGT GCATCACCAG 
CAGCGCCATT CGCCGCATCA TCATCATCAC CAGCAGCAGC AGCATCATCA GTTCCATCCC 
CACCACAATC ACCAGCATCA CTCCACATCC TCGCATCGGC TGTATTATCC GCCAGGTAGC 
TATGCCTCGC AAAAGTACAG CCAGCACGGC AGCTACTATC CCCACTTGCA GCAGTATGGC 
AATGAGACGC TACTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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fz4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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