rb cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	rb
Entrez Gene ID	31381
Full Name	ruby
Synonyms	AP-3beta,CG11427,Dmel\CG11427,Rb,apl6,beta3,cg11427
General protein information	Preferred Names ruby Names CG11427 gene product from transcript CG11427-RC CG11427-PA CG11427-PC CG11427-PD adaptor protein complex 3, beta subunit rb-PA rb-PC rb-PD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 4C7-4C7\|1-7.5 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001169193.2	NP_001162664.2	ruby, isoform D
NM_001272310.1	NP_001259239.1	ruby, isoform C
NM_080332.4	NP_525071.2	ruby, isoform A

Pathways from BioSystems

Homologies

Bos taurus (cattle)	AP3B1	NP_001070002.1
Schizosaccharomyces pombe (fission yeast)	apl6	NP_593796.1
Homo sapiens (human)	AP3B1	NP_003655.3
Pan troglodytes (chimpanzee)	AP3B1	XP_517689.2
	AP3B1	XP_002804484.1
Canis lupus familiaris (dog)	AP3B1	NP_001002974.1
Mus musculus (house mouse)	Ap3b1	NP_033810.2
Gallus gallus (chicken)	AP3B1	XP_003643003.1
Drosophila melanogaster (fruit fly)	rb	NP_525071.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that UTX is a suppressor of Notch- and Rb-dependent tumors in Drosophila melanogaster and may provide a model for UTX-dependent tumorigenesis in humans.
Title: The H3K27me3 demethylase dUTX is a suppressor of Notch- and Rb-dependent tumors in Drosophila.

The following rb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa00777	NM_001272310.1 Latest version!	Drosophila melanogaster ruby (rb), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa00777	NM_001169193.2 Latest version!	Drosophila melanogaster ruby (rb), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa00777	NM_080332.4 Latest version!	Drosophila melanogaster ruby (rb), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rb ( NM_001169193.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162664.2 Drosophila melanogaster ruby (rb), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa00777
Clone ID Related Accession (Same CDS sequence)	NM_001169193.2 , NM_001272310.1 , NM_080332.4
Accession Version	NM_001169193.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ruby, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001169193.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGCAGA ACTCGGCCAG CAATCCCTTC GCCATGTCCA CATATGTGGA GCGGCCCCAA 
ATGGGACTGG ACGTCGAATT CGGAGCGGAT CCTGCCAGCG GAGCAGCTTT CTTCCAATCC 
GATGGACGCA AGCATGATGA TCTCAAGCAA ATGTTGGACA GCAACAAGGA TGGACTAAAG 
CTGGAGGCCA TGAAGCGCAT CATTGGCATG ATTGCCCGCG GACGGGATGC CAGTGATCTG 
TTCCCGGCAG TCGTCAAGAA TGTGGTGTCC AAGAATATCG AGGTCAAGAA GTTGGTCTAC 
GTCTATTTGG TGCGATACGC CGAGGAGCAG CAGGACTTGG CCCTGCTATC CATTTCCACA 
TTTCAGAGGG CGCTCAAGGA TCCCAATCAA CTTATTCGCG CCTCGGCTCT GCGAGTGTTA 
TCCTCCATAA GGGTCAGCAT GATTGTGCCA ATCGTAATGC TGGCCATTCG GGATAGCGCC 
GCCGATTTGA GTCCATATGT GCGGAAAACA GCGGCACATG CCATCCCCAA GTTGTATTCT 
CTGGACGCGG ATCAAAAGGA TGAACTGGTC ATGGTCATTG AGAAGCTTTT ATCGGATCGC 
ACTACTCTAG TGGTGGGATC AGCGGTGATG GCCTTCGACG AGGTTTGTCC GGAACGTGTA 
GATTTGATAC ACAAGAACTA CCGCAAGCTG TGCAATTTCC TGCTGGATGT GGACGAATGG 
GGTCAGGTGA TCATCATTAA TATGTTGACC CGCTATGCGC GCACTCAGTT CGTTGATCCC 
AATGCGGATG ATGAGGATCT GGTCAACGAT GGCCTGGGCG AAACGCCCGT TAGTGAACGC 
TTCTACGACG AATCCTCGCA CTCCTCTTCC CACAGCGATG ATGGCAGTAG CGATGACGAA 
AAAAACAAGT CGAGTACGAA CAATAAAAAC AATGGCGGAG GAAATGGCAG TAGAACACCC 
AGTTCTCCGA GCAACAGCTA CCACATCGAT GTGGATCACC GCCTGCTGTT GAGGCAAACG 
AAACCATTGC TCCAATCGCG AAATGCCTCC GTGGTGATGG CAGTGGCGCA GCTCTATCAT 
CATGTGGCTC CCAAGAACGA AGTGCAATTG ATTGCCAAGG CGCTGATACG ACTGCTGCGA 
TCGCATAAGG AGGTTCAGAG CGTGGTCCTC AATTGCATTG CCTCGATGTC CACCAAACGA 
AAGGCGATCT TTGAGCCGCA CCTCAAGTCG TTCTTTGTGC GCACCAGCGA TCCCACGCAC 
ATAAAGCTTC TCAAGCTGGA TATTCTCACC AATCTGGCCT CGGCGGGCAG TATTTCGCTC 
ATTCTGCGCG AATTTCAAAC CTACATCTCC AGCAGCGATC GTTCGTTTGT GGCGGCCACG 
ATTCAGGCCA TTGGACGATG TGCATCTAGC ATTAAGGAGG TTACCGAGAC CTGCTTGAGT 
GGCCTGGTTC ATCTGCTGTC CAATCATGAC GAACACGTGG TGGCCGAGAG CGTGGTGGTG 
ATTAAGCGTC TTCTGCAAAC GAAAGCCGCC GAGCACTTTG AGATTATCAC CCAAATGGCC 
AAACTGATCG ATTATATCAA TGTGCCCGCA GCTAGGGCAG CCATCATTTG GCTTATAGGC 
GAATACAACG AGAAGGTGCC GCTGATTGCC CCGGATGTGC TGCGCAAAAT GGCCAAATCG 
TTTGTCGATG AGCAGGATGT GGTGAAGCTG CAGGTCCTAA ACTTAGGCGT TAAGCTCTAT 
CTGACCAATC CGGAGCAGAC ATCGTTGCTG TGTCAGTACG TCTTTACGCT GGCTCGCTAT 
GATCCCAACT ACGATGTACG CGATCGTGCC CGTTTTCTGC GGCAAATCAT CTTTCCGGCG 
AGCGGAACGA GCTCGGTGCT CAGCCAGCAT GCCCGCCAAG TATTTCTCGC CAGCAAACCG 
GCTCCGGTGC CGGAGAGTAA ATACCGGGAT GGCAATAACT TCCAGTTAGG ATCGTTGTCT 
CACTACCTGA ACATGCCAGC GGCTGGCTAC AAGGAACTGC CCGCCTTTCC GGTGATTCCA 
CCGGATTCAT CGGTACGCAA TATAGCTGGA TTTATGCAGG ACAAGCTGCC GGGTGAAGAC 
TCGCCATCCG GACATTCAAA GGATAAATCC GGCCGTGAAG GCGGTAAGGA AAAGGGTGCT 
GGTGGCGAGA AGGGCTTCCT CTCTGAGTCG GACGACAAGT CTTCCGCTTA CTCGGAATCA 
GGCAGCAGCA GTGGCAGTGG CACCAGCGAC AGTGAATCCG ATAGCGATGG CAGCGGGAGC 
AGCGATGAGG AGGAGGAGGA GGTGCAAAAG CAGCAGCCGA CCAAGGTTTC CTCGGTGAAG 
GAGCGGCTTA TCGATGCTGG AAGCAATGAG CTGGCCGCTA CTTCTTCGCC TGTAAAAGCA 
ACTATAAACA ACAATAACAA TGCGAGAGGT TCGTCAGGAA CATCGGACAG CGAGGATTCA 
TCAGCGTACA GTGGCAGTAG TAGTGATGAC AGCGCTTCCG GTAGCGATAA TGAAGTGAAA 
AAGAACGAAA CGAAAACGGA CACAGGCACA AAGCCGGAGA AGTTGAAAGA GAAGCACGAG 
AAACAGCCGG AGCAAACGGC TGTCAAGAGC AATCTGGACC TGCTACTCGA TCTGGATGAC 
ATTCCGCCCA TTGGTCCCGT GATGACGCCC TCGTTGGGAG GATTCCTGAC GCCTGGCACA 
CCAATGATGG CTGGACAGGC GGCGCCACTG CAGCCGCAGC ACGCCCGCAA TCGGGTGGAA 
TTGGTGGGTC CCTCGCACAT CGAGTTCAAG CACAAGGAGC TGCTTAACAA GGTCAGTGGC 
CATGGACTGC ATTTGGCCTA CCGTTTCACC CGCTCCCCGC ATCTGTACTC CTCGAGCATG 
TGCTCCATTG AGCTGCAGTT CCAGAATCGT GGCGAAAAAG AGATTACGGC CATTCGTCTC 
GGGCAAACAA CTCTGCCGGC GGGCATGCAA TTGAATGAGT TTGCGCCAAT CACTGTTCTC 
CAGCCCCAAC AAACAGCGAG TGGTGTGCTG GGCGTCGATT TCAATGATTC GACGCATGCT 
GTAGACCTGG AGCTTCTGTC AAGTGCGGGC AGTTCACGAC TGCAATTGAA ACCGCCGGTG 
GGCGAGCTGG TGAGATCCGT CCAGATCGGC GAGAGCTGTC ATCGCGAGGA GCGGGCCAAG 
CTGCGTGGAA TGAATGAGCA TCAGTGCGAG TTGCGTGGCC TGAGGCAGGA CCTAATCGAT 
GTGGTCGCAT TGCGGCAAAA GGTTTTCGAG TCCATCAACG TGGCGCATAC GCACAGCTCT 
TCCAGCGGTC AGCTACACTG TTTCGCCGGC CAAACCCTGA GCTCCAAAAG CCTCGTGCTA 
CTCACCCTCG ATTGGAAAAC AGAGGATGCC CTCACGCTGC TGGTAAACTG CGAGAAAATG 
GTCATTGGAT CCATGGTGCT CAACGAGCTG CGCAATGCTC TGCAGCTCAG TTTCGCCATG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162664.2
CDS	322..3804
Translation	MQQNSASNPFAMSTYVERPQMGLDVEFGADPASGAAFFQSDGRKHDDLKQMLDSNKDGLKLEAMKRIIGMIARGRDASDLFPAVVKNVVSKNIEVKKLVYVYLVRYAEEQQDLALLSISTFQRALKDPNQLIRASALRVLSSIRVSMIVPIVMLAIRDSAADLSPYVRKTAAHAIPKLYSLDADQKDELVMVIEKLLSDRTTLVVGSAVMAFDEVCPERVDLIHKNYRKLCNFLLDVDEWGQVIIINMLTRYARTQFVDPNADDEDLVNDGLGETPVSERFYDESSHSSSHSDDGSSDDEKNKSSTNNKNNGGGNGSRTPSSPSNSYHIDVDHRLLLRQTKPLLQSRNASVVMAVAQLYHHVAPKNEVQLIAKALIRLLRSHKEVQSVVLNCIASMSTKRKAIFEPHLKSFFVRTSDPTHIKLLKLDILTNLASAGSISLILREFQTYISSSDRSFVAATIQAIGRCASSIKEVTETCLSGLVHLLSNHDEHVVAESVVVIKRLLQTKAAEHFEIITQMAKLIDYINVPAARAAIIWLIGEYNEKVPLIAPDVLRKMAKSFVDEQDVVKLQVLNLGVKLYLTNPEQTSLLCQYVFTLARYDPNYDVRDRARFLRQIIFPASGTSSVLSQHARQVFLASKPAPVPESKYRDGNNFQLGSLSHYLNMPAAGYKELPAFPVIPPDSSVRNIAGFMQDKLPGEDSPSGHSKDKSGREGGKEKGAGGEKGFLSESDDKSSAYSESGSSSGSGTSDSESDSDGSGSSDEEEEEVQKQQPTKVSSVKERLIDAGSNELAATSSPVKATINNNNNARGSSGTSDSEDSSAYSGSSSDDSASGSDNEVKKNETKTDTGTKPEKLKEKHEKQPEQTAVKSNLDLLLDLDDIPPIGPVMTPSLGGFLTPGTPMMAGQAAPLQPQHARNRVELVGPSHIEFKHKELLNKVSGHGLHLAYRFTRSPHLYSSSMCSIELQFQNRGEKEITAIRLGQTTLPAGMQLNEFAPITVLQPQQTASGVLGVDFNDSTHAVDLELLSSAGSSRLQLKPPVGELVRSVQIGESCHREERAKLRGMNEHQCELRGLRQDLIDVVALRQKVFESINVAHTHSSSSGQLHCFAGQTLSSKSLVLLTLDWKTEDALTLLVNCEKMVIGSMVLNELRNALQLSFAM

Target ORF information:

RefSeq Version	NM_001169193.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ruby (rb), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169193.2

ORF Insert Sequence:

ATGCAGCAGA ACTCGGCCAG CAATCCCTTC GCCATGTCCA CATATGTGGA GCGGCCCCAA 
ATGGGACTGG ACGTCGAATT CGGAGCGGAT CCTGCCAGCG GAGCAGCTTT CTTCCAATCC 
GATGGACGCA AGCATGATGA TCTCAAGCAA ATGTTGGACA GCAACAAGGA TGGACTAAAG 
CTGGAGGCCA TGAAGCGCAT CATTGGCATG ATTGCCCGCG GACGGGATGC CAGTGATCTG 
TTCCCGGCAG TCGTCAAGAA TGTGGTGTCC AAGAATATCG AGGTCAAGAA GTTGGTCTAC 
GTCTATTTGG TGCGATACGC CGAGGAGCAG CAGGACTTGG CCCTGCTATC CATTTCCACA 
TTTCAGAGGG CGCTCAAGGA TCCCAATCAA CTTATTCGCG CCTCGGCTCT GCGAGTGTTA 
TCCTCCATAA GGGTCAGCAT GATTGTGCCA ATCGTAATGC TGGCCATTCG GGATAGCGCC 
GCCGATTTGA GTCCATATGT GCGGAAAACA GCGGCACATG CCATCCCCAA GTTGTATTCT 
CTGGACGCGG ATCAAAAGGA TGAACTGGTC ATGGTCATTG AGAAGCTTTT ATCGGATCGC 
ACTACTCTAG TGGTGGGATC AGCGGTGATG GCCTTCGACG AGGTTTGTCC GGAACGTGTA 
GATTTGATAC ACAAGAACTA CCGCAAGCTG TGCAATTTCC TGCTGGATGT GGACGAATGG 
GGTCAGGTGA TCATCATTAA TATGTTGACC CGCTATGCGC GCACTCAGTT CGTTGATCCC 
AATGCGGATG ATGAGGATCT GGTCAACGAT GGCCTGGGCG AAACGCCCGT TAGTGAACGC 
TTCTACGACG AATCCTCGCA CTCCTCTTCC CACAGCGATG ATGGCAGTAG CGATGACGAA 
AAAAACAAGT CGAGTACGAA CAATAAAAAC AATGGCGGAG GAAATGGCAG TAGAACACCC 
AGTTCTCCGA GCAACAGCTA CCACATCGAT GTGGATCACC GCCTGCTGTT GAGGCAAACG 
AAACCATTGC TCCAATCGCG AAATGCCTCC GTGGTGATGG CAGTGGCGCA GCTCTATCAT 
CATGTGGCTC CCAAGAACGA AGTGCAATTG ATTGCCAAGG CGCTGATACG ACTGCTGCGA 
TCGCATAAGG AGGTTCAGAG CGTGGTCCTC AATTGCATTG CCTCGATGTC CACCAAACGA 
AAGGCGATCT TTGAGCCGCA CCTCAAGTCG TTCTTTGTGC GCACCAGCGA TCCCACGCAC 
ATAAAGCTTC TCAAGCTGGA TATTCTCACC AATCTGGCCT CGGCGGGCAG TATTTCGCTC 
ATTCTGCGCG AATTTCAAAC CTACATCTCC AGCAGCGATC GTTCGTTTGT GGCGGCCACG 
ATTCAGGCCA TTGGACGATG TGCATCTAGC ATTAAGGAGG TTACCGAGAC CTGCTTGAGT 
GGCCTGGTTC ATCTGCTGTC CAATCATGAC GAACACGTGG TGGCCGAGAG CGTGGTGGTG 
ATTAAGCGTC TTCTGCAAAC GAAAGCCGCC GAGCACTTTG AGATTATCAC CCAAATGGCC 
AAACTGATCG ATTATATCAA TGTGCCCGCA GCTAGGGCAG CCATCATTTG GCTTATAGGC 
GAATACAACG AGAAGGTGCC GCTGATTGCC CCGGATGTGC TGCGCAAAAT GGCCAAATCG 
TTTGTCGATG AGCAGGATGT GGTGAAGCTG CAGGTCCTAA ACTTAGGCGT TAAGCTCTAT 
CTGACCAATC CGGAGCAGAC ATCGTTGCTG TGTCAGTACG TCTTTACGCT GGCTCGCTAT 
GATCCCAACT ACGATGTACG CGATCGTGCC CGTTTTCTGC GGCAAATCAT CTTTCCGGCG 
AGCGGAACGA GCTCGGTGCT CAGCCAGCAT GCCCGCCAAG TATTTCTCGC CAGCAAACCG 
GCTCCGGTGC CGGAGAGTAA ATACCGGGAT GGCAATAACT TCCAGTTAGG ATCGTTGTCT 
CACTACCTGA ACATGCCAGC GGCTGGCTAC AAGGAACTGC CCGCCTTTCC GGTGATTCCA 
CCGGATTCAT CGGTACGCAA TATAGCTGGA TTTATGCAGG ACAAGCTGCC GGGTGAAGAC 
TCGCCATCCG GACATTCAAA GGATAAATCC GGCCGTGAAG GCGGTAAGGA AAAGGGTGCT 
GGTGGCGAGA AGGGCTTCCT CTCTGAGTCG GACGACAAGT CTTCCGCTTA CTCGGAATCA 
GGCAGCAGCA GTGGCAGTGG CACCAGCGAC AGTGAATCCG ATAGCGATGG CAGCGGGAGC 
AGCGATGAGG AGGAGGAGGA GGTGCAAAAG CAGCAGCCGA CCAAGGTTTC CTCGGTGAAG 
GAGCGGCTTA TCGATGCTGG AAGCAATGAG CTGGCCGCTA CTTCTTCGCC TGTAAAAGCA 
ACTATAAACA ACAATAACAA TGCGAGAGGT TCGTCAGGAA CATCGGACAG CGAGGATTCA 
TCAGCGTACA GTGGCAGTAG TAGTGATGAC AGCGCTTCCG GTAGCGATAA TGAAGTGAAA 
AAGAACGAAA CGAAAACGGA CACAGGCACA AAGCCGGAGA AGTTGAAAGA GAAGCACGAG 
AAACAGCCGG AGCAAACGGC TGTCAAGAGC AATCTGGACC TGCTACTCGA TCTGGATGAC 
ATTCCGCCCA TTGGTCCCGT GATGACGCCC TCGTTGGGAG GATTCCTGAC GCCTGGCACA 
CCAATGATGG CTGGACAGGC GGCGCCACTG CAGCCGCAGC ACGCCCGCAA TCGGGTGGAA 
TTGGTGGGTC CCTCGCACAT CGAGTTCAAG CACAAGGAGC TGCTTAACAA GGTCAGTGGC 
CATGGACTGC ATTTGGCCTA CCGTTTCACC CGCTCCCCGC ATCTGTACTC CTCGAGCATG 
TGCTCCATTG AGCTGCAGTT CCAGAATCGT GGCGAAAAAG AGATTACGGC CATTCGTCTC 
GGGCAAACAA CTCTGCCGGC GGGCATGCAA TTGAATGAGT TTGCGCCAAT CACTGTTCTC 
CAGCCCCAAC AAACAGCGAG TGGTGTGCTG GGCGTCGATT TCAATGATTC GACGCATGCT 
GTAGACCTGG AGCTTCTGTC AAGTGCGGGC AGTTCACGAC TGCAATTGAA ACCGCCGGTG 
GGCGAGCTGG TGAGATCCGT CCAGATCGGC GAGAGCTGTC ATCGCGAGGA GCGGGCCAAG 
CTGCGTGGAA TGAATGAGCA TCAGTGCGAG TTGCGTGGCC TGAGGCAGGA CCTAATCGAT 
GTGGTCGCAT TGCGGCAAAA GGTTTTCGAG TCCATCAACG TGGCGCATAC GCACAGCTCT 
TCCAGCGGTC AGCTACACTG TTTCGCCGGC CAAACCCTGA GCTCCAAAAG CCTCGTGCTA 
CTCACCCTCG ATTGGAAAAC AGAGGATGCC CTCACGCTGC TGGTAAACTG CGAGAAAATG 
GTCATTGGAT CCATGGTGCT CAACGAGCTG CGCAATGCTC TGCAGCTCAG TTTCGCCATG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rb ( NM_001272310.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259239.1 Drosophila melanogaster ruby (rb), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa00777
Clone ID Related Accession (Same CDS sequence)	NM_001169193.2 , NM_001272310.1 , NM_080332.4
Accession Version	NM_001272310.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ruby, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGCAGA ACTCGGCCAG CAATCCCTTC GCCATGTCCA CATATGTGGA GCGGCCCCAA 
ATGGGACTGG ACGTCGAATT CGGAGCGGAT CCTGCCAGCG GAGCAGCTTT CTTCCAATCC 
GATGGACGCA AGCATGATGA TCTCAAGCAA ATGTTGGACA GCAACAAGGA TGGACTAAAG 
CTGGAGGCCA TGAAGCGCAT CATTGGCATG ATTGCCCGCG GACGGGATGC CAGTGATCTG 
TTCCCGGCAG TCGTCAAGAA TGTGGTGTCC AAGAATATCG AGGTCAAGAA GTTGGTCTAC 
GTCTATTTGG TGCGATACGC CGAGGAGCAG CAGGACTTGG CCCTGCTATC CATTTCCACA 
TTTCAGAGGG CGCTCAAGGA TCCCAATCAA CTTATTCGCG CCTCGGCTCT GCGAGTGTTA 
TCCTCCATAA GGGTCAGCAT GATTGTGCCA ATCGTAATGC TGGCCATTCG GGATAGCGCC 
GCCGATTTGA GTCCATATGT GCGGAAAACA GCGGCACATG CCATCCCCAA GTTGTATTCT 
CTGGACGCGG ATCAAAAGGA TGAACTGGTC ATGGTCATTG AGAAGCTTTT ATCGGATCGC 
ACTACTCTAG TGGTGGGATC AGCGGTGATG GCCTTCGACG AGGTTTGTCC GGAACGTGTA 
GATTTGATAC ACAAGAACTA CCGCAAGCTG TGCAATTTCC TGCTGGATGT GGACGAATGG 
GGTCAGGTGA TCATCATTAA TATGTTGACC CGCTATGCGC GCACTCAGTT CGTTGATCCC 
AATGCGGATG ATGAGGATCT GGTCAACGAT GGCCTGGGCG AAACGCCCGT TAGTGAACGC 
TTCTACGACG AATCCTCGCA CTCCTCTTCC CACAGCGATG ATGGCAGTAG CGATGACGAA 
AAAAACAAGT CGAGTACGAA CAATAAAAAC AATGGCGGAG GAAATGGCAG TAGAACACCC 
AGTTCTCCGA GCAACAGCTA CCACATCGAT GTGGATCACC GCCTGCTGTT GAGGCAAACG 
AAACCATTGC TCCAATCGCG AAATGCCTCC GTGGTGATGG CAGTGGCGCA GCTCTATCAT 
CATGTGGCTC CCAAGAACGA AGTGCAATTG ATTGCCAAGG CGCTGATACG ACTGCTGCGA 
TCGCATAAGG AGGTTCAGAG CGTGGTCCTC AATTGCATTG CCTCGATGTC CACCAAACGA 
AAGGCGATCT TTGAGCCGCA CCTCAAGTCG TTCTTTGTGC GCACCAGCGA TCCCACGCAC 
ATAAAGCTTC TCAAGCTGGA TATTCTCACC AATCTGGCCT CGGCGGGCAG TATTTCGCTC 
ATTCTGCGCG AATTTCAAAC CTACATCTCC AGCAGCGATC GTTCGTTTGT GGCGGCCACG 
ATTCAGGCCA TTGGACGATG TGCATCTAGC ATTAAGGAGG TTACCGAGAC CTGCTTGAGT 
GGCCTGGTTC ATCTGCTGTC CAATCATGAC GAACACGTGG TGGCCGAGAG CGTGGTGGTG 
ATTAAGCGTC TTCTGCAAAC GAAAGCCGCC GAGCACTTTG AGATTATCAC CCAAATGGCC 
AAACTGATCG ATTATATCAA TGTGCCCGCA GCTAGGGCAG CCATCATTTG GCTTATAGGC 
GAATACAACG AGAAGGTGCC GCTGATTGCC CCGGATGTGC TGCGCAAAAT GGCCAAATCG 
TTTGTCGATG AGCAGGATGT GGTGAAGCTG CAGGTCCTAA ACTTAGGCGT TAAGCTCTAT 
CTGACCAATC CGGAGCAGAC ATCGTTGCTG TGTCAGTACG TCTTTACGCT GGCTCGCTAT 
GATCCCAACT ACGATGTACG CGATCGTGCC CGTTTTCTGC GGCAAATCAT CTTTCCGGCG 
AGCGGAACGA GCTCGGTGCT CAGCCAGCAT GCCCGCCAAG TATTTCTCGC CAGCAAACCG 
GCTCCGGTGC CGGAGAGTAA ATACCGGGAT GGCAATAACT TCCAGTTAGG ATCGTTGTCT 
CACTACCTGA ACATGCCAGC GGCTGGCTAC AAGGAACTGC CCGCCTTTCC GGTGATTCCA 
CCGGATTCAT CGGTACGCAA TATAGCTGGA TTTATGCAGG ACAAGCTGCC GGGTGAAGAC 
TCGCCATCCG GACATTCAAA GGATAAATCC GGCCGTGAAG GCGGTAAGGA AAAGGGTGCT 
GGTGGCGAGA AGGGCTTCCT CTCTGAGTCG GACGACAAGT CTTCCGCTTA CTCGGAATCA 
GGCAGCAGCA GTGGCAGTGG CACCAGCGAC AGTGAATCCG ATAGCGATGG CAGCGGGAGC 
AGCGATGAGG AGGAGGAGGA GGTGCAAAAG CAGCAGCCGA CCAAGGTTTC CTCGGTGAAG 
GAGCGGCTTA TCGATGCTGG AAGCAATGAG CTGGCCGCTA CTTCTTCGCC TGTAAAAGCA 
ACTATAAACA ACAATAACAA TGCGAGAGGT TCGTCAGGAA CATCGGACAG CGAGGATTCA 
TCAGCGTACA GTGGCAGTAG TAGTGATGAC AGCGCTTCCG GTAGCGATAA TGAAGTGAAA 
AAGAACGAAA CGAAAACGGA CACAGGCACA AAGCCGGAGA AGTTGAAAGA GAAGCACGAG 
AAACAGCCGG AGCAAACGGC TGTCAAGAGC AATCTGGACC TGCTACTCGA TCTGGATGAC 
ATTCCGCCCA TTGGTCCCGT GATGACGCCC TCGTTGGGAG GATTCCTGAC GCCTGGCACA 
CCAATGATGG CTGGACAGGC GGCGCCACTG CAGCCGCAGC ACGCCCGCAA TCGGGTGGAA 
TTGGTGGGTC CCTCGCACAT CGAGTTCAAG CACAAGGAGC TGCTTAACAA GGTCAGTGGC 
CATGGACTGC ATTTGGCCTA CCGTTTCACC CGCTCCCCGC ATCTGTACTC CTCGAGCATG 
TGCTCCATTG AGCTGCAGTT CCAGAATCGT GGCGAAAAAG AGATTACGGC CATTCGTCTC 
GGGCAAACAA CTCTGCCGGC GGGCATGCAA TTGAATGAGT TTGCGCCAAT CACTGTTCTC 
CAGCCCCAAC AAACAGCGAG TGGTGTGCTG GGCGTCGATT TCAATGATTC GACGCATGCT 
GTAGACCTGG AGCTTCTGTC AAGTGCGGGC AGTTCACGAC TGCAATTGAA ACCGCCGGTG 
GGCGAGCTGG TGAGATCCGT CCAGATCGGC GAGAGCTGTC ATCGCGAGGA GCGGGCCAAG 
CTGCGTGGAA TGAATGAGCA TCAGTGCGAG TTGCGTGGCC TGAGGCAGGA CCTAATCGAT 
GTGGTCGCAT TGCGGCAAAA GGTTTTCGAG TCCATCAACG TGGCGCATAC GCACAGCTCT 
TCCAGCGGTC AGCTACACTG TTTCGCCGGC CAAACCCTGA GCTCCAAAAG CCTCGTGCTA 
CTCACCCTCG ATTGGAAAAC AGAGGATGCC CTCACGCTGC TGGTAAACTG CGAGAAAATG 
GTCATTGGAT CCATGGTGCT CAACGAGCTG CGCAATGCTC TGCAGCTCAG TTTCGCCATG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259239.1
CDS	322..3804
Translation	MQQNSASNPFAMSTYVERPQMGLDVEFGADPASGAAFFQSDGRKHDDLKQMLDSNKDGLKLEAMKRIIGMIARGRDASDLFPAVVKNVVSKNIEVKKLVYVYLVRYAEEQQDLALLSISTFQRALKDPNQLIRASALRVLSSIRVSMIVPIVMLAIRDSAADLSPYVRKTAAHAIPKLYSLDADQKDELVMVIEKLLSDRTTLVVGSAVMAFDEVCPERVDLIHKNYRKLCNFLLDVDEWGQVIIINMLTRYARTQFVDPNADDEDLVNDGLGETPVSERFYDESSHSSSHSDDGSSDDEKNKSSTNNKNNGGGNGSRTPSSPSNSYHIDVDHRLLLRQTKPLLQSRNASVVMAVAQLYHHVAPKNEVQLIAKALIRLLRSHKEVQSVVLNCIASMSTKRKAIFEPHLKSFFVRTSDPTHIKLLKLDILTNLASAGSISLILREFQTYISSSDRSFVAATIQAIGRCASSIKEVTETCLSGLVHLLSNHDEHVVAESVVVIKRLLQTKAAEHFEIITQMAKLIDYINVPAARAAIIWLIGEYNEKVPLIAPDVLRKMAKSFVDEQDVVKLQVLNLGVKLYLTNPEQTSLLCQYVFTLARYDPNYDVRDRARFLRQIIFPASGTSSVLSQHARQVFLASKPAPVPESKYRDGNNFQLGSLSHYLNMPAAGYKELPAFPVIPPDSSVRNIAGFMQDKLPGEDSPSGHSKDKSGREGGKEKGAGGEKGFLSESDDKSSAYSESGSSSGSGTSDSESDSDGSGSSDEEEEEVQKQQPTKVSSVKERLIDAGSNELAATSSPVKATINNNNNARGSSGTSDSEDSSAYSGSSSDDSASGSDNEVKKNETKTDTGTKPEKLKEKHEKQPEQTAVKSNLDLLLDLDDIPPIGPVMTPSLGGFLTPGTPMMAGQAAPLQPQHARNRVELVGPSHIEFKHKELLNKVSGHGLHLAYRFTRSPHLYSSSMCSIELQFQNRGEKEITAIRLGQTTLPAGMQLNEFAPITVLQPQQTASGVLGVDFNDSTHAVDLELLSSAGSSRLQLKPPVGELVRSVQIGESCHREERAKLRGMNEHQCELRGLRQDLIDVVALRQKVFESINVAHTHSSSSGQLHCFAGQTLSSKSLVLLTLDWKTEDALTLLVNCEKMVIGSMVLNELRNALQLSFAM

Target ORF information:

RefSeq Version	NM_001272310.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ruby (rb), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272310.1

ORF Insert Sequence:

ATGCAGCAGA ACTCGGCCAG CAATCCCTTC GCCATGTCCA CATATGTGGA GCGGCCCCAA 
ATGGGACTGG ACGTCGAATT CGGAGCGGAT CCTGCCAGCG GAGCAGCTTT CTTCCAATCC 
GATGGACGCA AGCATGATGA TCTCAAGCAA ATGTTGGACA GCAACAAGGA TGGACTAAAG 
CTGGAGGCCA TGAAGCGCAT CATTGGCATG ATTGCCCGCG GACGGGATGC CAGTGATCTG 
TTCCCGGCAG TCGTCAAGAA TGTGGTGTCC AAGAATATCG AGGTCAAGAA GTTGGTCTAC 
GTCTATTTGG TGCGATACGC CGAGGAGCAG CAGGACTTGG CCCTGCTATC CATTTCCACA 
TTTCAGAGGG CGCTCAAGGA TCCCAATCAA CTTATTCGCG CCTCGGCTCT GCGAGTGTTA 
TCCTCCATAA GGGTCAGCAT GATTGTGCCA ATCGTAATGC TGGCCATTCG GGATAGCGCC 
GCCGATTTGA GTCCATATGT GCGGAAAACA GCGGCACATG CCATCCCCAA GTTGTATTCT 
CTGGACGCGG ATCAAAAGGA TGAACTGGTC ATGGTCATTG AGAAGCTTTT ATCGGATCGC 
ACTACTCTAG TGGTGGGATC AGCGGTGATG GCCTTCGACG AGGTTTGTCC GGAACGTGTA 
GATTTGATAC ACAAGAACTA CCGCAAGCTG TGCAATTTCC TGCTGGATGT GGACGAATGG 
GGTCAGGTGA TCATCATTAA TATGTTGACC CGCTATGCGC GCACTCAGTT CGTTGATCCC 
AATGCGGATG ATGAGGATCT GGTCAACGAT GGCCTGGGCG AAACGCCCGT TAGTGAACGC 
TTCTACGACG AATCCTCGCA CTCCTCTTCC CACAGCGATG ATGGCAGTAG CGATGACGAA 
AAAAACAAGT CGAGTACGAA CAATAAAAAC AATGGCGGAG GAAATGGCAG TAGAACACCC 
AGTTCTCCGA GCAACAGCTA CCACATCGAT GTGGATCACC GCCTGCTGTT GAGGCAAACG 
AAACCATTGC TCCAATCGCG AAATGCCTCC GTGGTGATGG CAGTGGCGCA GCTCTATCAT 
CATGTGGCTC CCAAGAACGA AGTGCAATTG ATTGCCAAGG CGCTGATACG ACTGCTGCGA 
TCGCATAAGG AGGTTCAGAG CGTGGTCCTC AATTGCATTG CCTCGATGTC CACCAAACGA 
AAGGCGATCT TTGAGCCGCA CCTCAAGTCG TTCTTTGTGC GCACCAGCGA TCCCACGCAC 
ATAAAGCTTC TCAAGCTGGA TATTCTCACC AATCTGGCCT CGGCGGGCAG TATTTCGCTC 
ATTCTGCGCG AATTTCAAAC CTACATCTCC AGCAGCGATC GTTCGTTTGT GGCGGCCACG 
ATTCAGGCCA TTGGACGATG TGCATCTAGC ATTAAGGAGG TTACCGAGAC CTGCTTGAGT 
GGCCTGGTTC ATCTGCTGTC CAATCATGAC GAACACGTGG TGGCCGAGAG CGTGGTGGTG 
ATTAAGCGTC TTCTGCAAAC GAAAGCCGCC GAGCACTTTG AGATTATCAC CCAAATGGCC 
AAACTGATCG ATTATATCAA TGTGCCCGCA GCTAGGGCAG CCATCATTTG GCTTATAGGC 
GAATACAACG AGAAGGTGCC GCTGATTGCC CCGGATGTGC TGCGCAAAAT GGCCAAATCG 
TTTGTCGATG AGCAGGATGT GGTGAAGCTG CAGGTCCTAA ACTTAGGCGT TAAGCTCTAT 
CTGACCAATC CGGAGCAGAC ATCGTTGCTG TGTCAGTACG TCTTTACGCT GGCTCGCTAT 
GATCCCAACT ACGATGTACG CGATCGTGCC CGTTTTCTGC GGCAAATCAT CTTTCCGGCG 
AGCGGAACGA GCTCGGTGCT CAGCCAGCAT GCCCGCCAAG TATTTCTCGC CAGCAAACCG 
GCTCCGGTGC CGGAGAGTAA ATACCGGGAT GGCAATAACT TCCAGTTAGG ATCGTTGTCT 
CACTACCTGA ACATGCCAGC GGCTGGCTAC AAGGAACTGC CCGCCTTTCC GGTGATTCCA 
CCGGATTCAT CGGTACGCAA TATAGCTGGA TTTATGCAGG ACAAGCTGCC GGGTGAAGAC 
TCGCCATCCG GACATTCAAA GGATAAATCC GGCCGTGAAG GCGGTAAGGA AAAGGGTGCT 
GGTGGCGAGA AGGGCTTCCT CTCTGAGTCG GACGACAAGT CTTCCGCTTA CTCGGAATCA 
GGCAGCAGCA GTGGCAGTGG CACCAGCGAC AGTGAATCCG ATAGCGATGG CAGCGGGAGC 
AGCGATGAGG AGGAGGAGGA GGTGCAAAAG CAGCAGCCGA CCAAGGTTTC CTCGGTGAAG 
GAGCGGCTTA TCGATGCTGG AAGCAATGAG CTGGCCGCTA CTTCTTCGCC TGTAAAAGCA 
ACTATAAACA ACAATAACAA TGCGAGAGGT TCGTCAGGAA CATCGGACAG CGAGGATTCA 
TCAGCGTACA GTGGCAGTAG TAGTGATGAC AGCGCTTCCG GTAGCGATAA TGAAGTGAAA 
AAGAACGAAA CGAAAACGGA CACAGGCACA AAGCCGGAGA AGTTGAAAGA GAAGCACGAG 
AAACAGCCGG AGCAAACGGC TGTCAAGAGC AATCTGGACC TGCTACTCGA TCTGGATGAC 
ATTCCGCCCA TTGGTCCCGT GATGACGCCC TCGTTGGGAG GATTCCTGAC GCCTGGCACA 
CCAATGATGG CTGGACAGGC GGCGCCACTG CAGCCGCAGC ACGCCCGCAA TCGGGTGGAA 
TTGGTGGGTC CCTCGCACAT CGAGTTCAAG CACAAGGAGC TGCTTAACAA GGTCAGTGGC 
CATGGACTGC ATTTGGCCTA CCGTTTCACC CGCTCCCCGC ATCTGTACTC CTCGAGCATG 
TGCTCCATTG AGCTGCAGTT CCAGAATCGT GGCGAAAAAG AGATTACGGC CATTCGTCTC 
GGGCAAACAA CTCTGCCGGC GGGCATGCAA TTGAATGAGT TTGCGCCAAT CACTGTTCTC 
CAGCCCCAAC AAACAGCGAG TGGTGTGCTG GGCGTCGATT TCAATGATTC GACGCATGCT 
GTAGACCTGG AGCTTCTGTC AAGTGCGGGC AGTTCACGAC TGCAATTGAA ACCGCCGGTG 
GGCGAGCTGG TGAGATCCGT CCAGATCGGC GAGAGCTGTC ATCGCGAGGA GCGGGCCAAG 
CTGCGTGGAA TGAATGAGCA TCAGTGCGAG TTGCGTGGCC TGAGGCAGGA CCTAATCGAT 
GTGGTCGCAT TGCGGCAAAA GGTTTTCGAG TCCATCAACG TGGCGCATAC GCACAGCTCT 
TCCAGCGGTC AGCTACACTG TTTCGCCGGC CAAACCCTGA GCTCCAAAAG CCTCGTGCTA 
CTCACCCTCG ATTGGAAAAC AGAGGATGCC CTCACGCTGC TGGTAAACTG CGAGAAAATG 
GTCATTGGAT CCATGGTGCT CAACGAGCTG CGCAATGCTC TGCAGCTCAG TTTCGCCATG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rb ( NM_080332.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_525071.2 Drosophila melanogaster ruby (rb), transcript variant A, mRNA.

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CloneID	OFa00777
Clone ID Related Accession (Same CDS sequence)	NM_001169193.2 , NM_001272310.1 , NM_080332.4
Accession Version	NM_080332.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	ruby, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_080332.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCAGCAGA ACTCGGCCAG CAATCCCTTC GCCATGTCCA CATATGTGGA GCGGCCCCAA 
ATGGGACTGG ACGTCGAATT CGGAGCGGAT CCTGCCAGCG GAGCAGCTTT CTTCCAATCC 
GATGGACGCA AGCATGATGA TCTCAAGCAA ATGTTGGACA GCAACAAGGA TGGACTAAAG 
CTGGAGGCCA TGAAGCGCAT CATTGGCATG ATTGCCCGCG GACGGGATGC CAGTGATCTG 
TTCCCGGCAG TCGTCAAGAA TGTGGTGTCC AAGAATATCG AGGTCAAGAA GTTGGTCTAC 
GTCTATTTGG TGCGATACGC CGAGGAGCAG CAGGACTTGG CCCTGCTATC CATTTCCACA 
TTTCAGAGGG CGCTCAAGGA TCCCAATCAA CTTATTCGCG CCTCGGCTCT GCGAGTGTTA 
TCCTCCATAA GGGTCAGCAT GATTGTGCCA ATCGTAATGC TGGCCATTCG GGATAGCGCC 
GCCGATTTGA GTCCATATGT GCGGAAAACA GCGGCACATG CCATCCCCAA GTTGTATTCT 
CTGGACGCGG ATCAAAAGGA TGAACTGGTC ATGGTCATTG AGAAGCTTTT ATCGGATCGC 
ACTACTCTAG TGGTGGGATC AGCGGTGATG GCCTTCGACG AGGTTTGTCC GGAACGTGTA 
GATTTGATAC ACAAGAACTA CCGCAAGCTG TGCAATTTCC TGCTGGATGT GGACGAATGG 
GGTCAGGTGA TCATCATTAA TATGTTGACC CGCTATGCGC GCACTCAGTT CGTTGATCCC 
AATGCGGATG ATGAGGATCT GGTCAACGAT GGCCTGGGCG AAACGCCCGT TAGTGAACGC 
TTCTACGACG AATCCTCGCA CTCCTCTTCC CACAGCGATG ATGGCAGTAG CGATGACGAA 
AAAAACAAGT CGAGTACGAA CAATAAAAAC AATGGCGGAG GAAATGGCAG TAGAACACCC 
AGTTCTCCGA GCAACAGCTA CCACATCGAT GTGGATCACC GCCTGCTGTT GAGGCAAACG 
AAACCATTGC TCCAATCGCG AAATGCCTCC GTGGTGATGG CAGTGGCGCA GCTCTATCAT 
CATGTGGCTC CCAAGAACGA AGTGCAATTG ATTGCCAAGG CGCTGATACG ACTGCTGCGA 
TCGCATAAGG AGGTTCAGAG CGTGGTCCTC AATTGCATTG CCTCGATGTC CACCAAACGA 
AAGGCGATCT TTGAGCCGCA CCTCAAGTCG TTCTTTGTGC GCACCAGCGA TCCCACGCAC 
ATAAAGCTTC TCAAGCTGGA TATTCTCACC AATCTGGCCT CGGCGGGCAG TATTTCGCTC 
ATTCTGCGCG AATTTCAAAC CTACATCTCC AGCAGCGATC GTTCGTTTGT GGCGGCCACG 
ATTCAGGCCA TTGGACGATG TGCATCTAGC ATTAAGGAGG TTACCGAGAC CTGCTTGAGT 
GGCCTGGTTC ATCTGCTGTC CAATCATGAC GAACACGTGG TGGCCGAGAG CGTGGTGGTG 
ATTAAGCGTC TTCTGCAAAC GAAAGCCGCC GAGCACTTTG AGATTATCAC CCAAATGGCC 
AAACTGATCG ATTATATCAA TGTGCCCGCA GCTAGGGCAG CCATCATTTG GCTTATAGGC 
GAATACAACG AGAAGGTGCC GCTGATTGCC CCGGATGTGC TGCGCAAAAT GGCCAAATCG 
TTTGTCGATG AGCAGGATGT GGTGAAGCTG CAGGTCCTAA ACTTAGGCGT TAAGCTCTAT 
CTGACCAATC CGGAGCAGAC ATCGTTGCTG TGTCAGTACG TCTTTACGCT GGCTCGCTAT 
GATCCCAACT ACGATGTACG CGATCGTGCC CGTTTTCTGC GGCAAATCAT CTTTCCGGCG 
AGCGGAACGA GCTCGGTGCT CAGCCAGCAT GCCCGCCAAG TATTTCTCGC CAGCAAACCG 
GCTCCGGTGC CGGAGAGTAA ATACCGGGAT GGCAATAACT TCCAGTTAGG ATCGTTGTCT 
CACTACCTGA ACATGCCAGC GGCTGGCTAC AAGGAACTGC CCGCCTTTCC GGTGATTCCA 
CCGGATTCAT CGGTACGCAA TATAGCTGGA TTTATGCAGG ACAAGCTGCC GGGTGAAGAC 
TCGCCATCCG GACATTCAAA GGATAAATCC GGCCGTGAAG GCGGTAAGGA AAAGGGTGCT 
GGTGGCGAGA AGGGCTTCCT CTCTGAGTCG GACGACAAGT CTTCCGCTTA CTCGGAATCA 
GGCAGCAGCA GTGGCAGTGG CACCAGCGAC AGTGAATCCG ATAGCGATGG CAGCGGGAGC 
AGCGATGAGG AGGAGGAGGA GGTGCAAAAG CAGCAGCCGA CCAAGGTTTC CTCGGTGAAG 
GAGCGGCTTA TCGATGCTGG AAGCAATGAG CTGGCCGCTA CTTCTTCGCC TGTAAAAGCA 
ACTATAAACA ACAATAACAA TGCGAGAGGT TCGTCAGGAA CATCGGACAG CGAGGATTCA 
TCAGCGTACA GTGGCAGTAG TAGTGATGAC AGCGCTTCCG GTAGCGATAA TGAAGTGAAA 
AAGAACGAAA CGAAAACGGA CACAGGCACA AAGCCGGAGA AGTTGAAAGA GAAGCACGAG 
AAACAGCCGG AGCAAACGGC TGTCAAGAGC AATCTGGACC TGCTACTCGA TCTGGATGAC 
ATTCCGCCCA TTGGTCCCGT GATGACGCCC TCGTTGGGAG GATTCCTGAC GCCTGGCACA 
CCAATGATGG CTGGACAGGC GGCGCCACTG CAGCCGCAGC ACGCCCGCAA TCGGGTGGAA 
TTGGTGGGTC CCTCGCACAT CGAGTTCAAG CACAAGGAGC TGCTTAACAA GGTCAGTGGC 
CATGGACTGC ATTTGGCCTA CCGTTTCACC CGCTCCCCGC ATCTGTACTC CTCGAGCATG 
TGCTCCATTG AGCTGCAGTT CCAGAATCGT GGCGAAAAAG AGATTACGGC CATTCGTCTC 
GGGCAAACAA CTCTGCCGGC GGGCATGCAA TTGAATGAGT TTGCGCCAAT CACTGTTCTC 
CAGCCCCAAC AAACAGCGAG TGGTGTGCTG GGCGTCGATT TCAATGATTC GACGCATGCT 
GTAGACCTGG AGCTTCTGTC AAGTGCGGGC AGTTCACGAC TGCAATTGAA ACCGCCGGTG 
GGCGAGCTGG TGAGATCCGT CCAGATCGGC GAGAGCTGTC ATCGCGAGGA GCGGGCCAAG 
CTGCGTGGAA TGAATGAGCA TCAGTGCGAG TTGCGTGGCC TGAGGCAGGA CCTAATCGAT 
GTGGTCGCAT TGCGGCAAAA GGTTTTCGAG TCCATCAACG TGGCGCATAC GCACAGCTCT 
TCCAGCGGTC AGCTACACTG TTTCGCCGGC CAAACCCTGA GCTCCAAAAG CCTCGTGCTA 
CTCACCCTCG ATTGGAAAAC AGAGGATGCC CTCACGCTGC TGGTAAACTG CGAGAAAATG 
GTCATTGGAT CCATGGTGCT CAACGAGCTG CGCAATGCTC TGCAGCTCAG TTTCGCCATG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_525071.2
CDS	322..3804
Translation	MQQNSASNPFAMSTYVERPQMGLDVEFGADPASGAAFFQSDGRKHDDLKQMLDSNKDGLKLEAMKRIIGMIARGRDASDLFPAVVKNVVSKNIEVKKLVYVYLVRYAEEQQDLALLSISTFQRALKDPNQLIRASALRVLSSIRVSMIVPIVMLAIRDSAADLSPYVRKTAAHAIPKLYSLDADQKDELVMVIEKLLSDRTTLVVGSAVMAFDEVCPERVDLIHKNYRKLCNFLLDVDEWGQVIIINMLTRYARTQFVDPNADDEDLVNDGLGETPVSERFYDESSHSSSHSDDGSSDDEKNKSSTNNKNNGGGNGSRTPSSPSNSYHIDVDHRLLLRQTKPLLQSRNASVVMAVAQLYHHVAPKNEVQLIAKALIRLLRSHKEVQSVVLNCIASMSTKRKAIFEPHLKSFFVRTSDPTHIKLLKLDILTNLASAGSISLILREFQTYISSSDRSFVAATIQAIGRCASSIKEVTETCLSGLVHLLSNHDEHVVAESVVVIKRLLQTKAAEHFEIITQMAKLIDYINVPAARAAIIWLIGEYNEKVPLIAPDVLRKMAKSFVDEQDVVKLQVLNLGVKLYLTNPEQTSLLCQYVFTLARYDPNYDVRDRARFLRQIIFPASGTSSVLSQHARQVFLASKPAPVPESKYRDGNNFQLGSLSHYLNMPAAGYKELPAFPVIPPDSSVRNIAGFMQDKLPGEDSPSGHSKDKSGREGGKEKGAGGEKGFLSESDDKSSAYSESGSSSGSGTSDSESDSDGSGSSDEEEEEVQKQQPTKVSSVKERLIDAGSNELAATSSPVKATINNNNNARGSSGTSDSEDSSAYSGSSSDDSASGSDNEVKKNETKTDTGTKPEKLKEKHEKQPEQTAVKSNLDLLLDLDDIPPIGPVMTPSLGGFLTPGTPMMAGQAAPLQPQHARNRVELVGPSHIEFKHKELLNKVSGHGLHLAYRFTRSPHLYSSSMCSIELQFQNRGEKEITAIRLGQTTLPAGMQLNEFAPITVLQPQQTASGVLGVDFNDSTHAVDLELLSSAGSSRLQLKPPVGELVRSVQIGESCHREERAKLRGMNEHQCELRGLRQDLIDVVALRQKVFESINVAHTHSSSSGQLHCFAGQTLSSKSLVLLTLDWKTEDALTLLVNCEKMVIGSMVLNELRNALQLSFAM

Target ORF information:

RefSeq Version	NM_080332.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster ruby (rb), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080332.4

ORF Insert Sequence:

ATGCAGCAGA ACTCGGCCAG CAATCCCTTC GCCATGTCCA CATATGTGGA GCGGCCCCAA 
ATGGGACTGG ACGTCGAATT CGGAGCGGAT CCTGCCAGCG GAGCAGCTTT CTTCCAATCC 
GATGGACGCA AGCATGATGA TCTCAAGCAA ATGTTGGACA GCAACAAGGA TGGACTAAAG 
CTGGAGGCCA TGAAGCGCAT CATTGGCATG ATTGCCCGCG GACGGGATGC CAGTGATCTG 
TTCCCGGCAG TCGTCAAGAA TGTGGTGTCC AAGAATATCG AGGTCAAGAA GTTGGTCTAC 
GTCTATTTGG TGCGATACGC CGAGGAGCAG CAGGACTTGG CCCTGCTATC CATTTCCACA 
TTTCAGAGGG CGCTCAAGGA TCCCAATCAA CTTATTCGCG CCTCGGCTCT GCGAGTGTTA 
TCCTCCATAA GGGTCAGCAT GATTGTGCCA ATCGTAATGC TGGCCATTCG GGATAGCGCC 
GCCGATTTGA GTCCATATGT GCGGAAAACA GCGGCACATG CCATCCCCAA GTTGTATTCT 
CTGGACGCGG ATCAAAAGGA TGAACTGGTC ATGGTCATTG AGAAGCTTTT ATCGGATCGC 
ACTACTCTAG TGGTGGGATC AGCGGTGATG GCCTTCGACG AGGTTTGTCC GGAACGTGTA 
GATTTGATAC ACAAGAACTA CCGCAAGCTG TGCAATTTCC TGCTGGATGT GGACGAATGG 
GGTCAGGTGA TCATCATTAA TATGTTGACC CGCTATGCGC GCACTCAGTT CGTTGATCCC 
AATGCGGATG ATGAGGATCT GGTCAACGAT GGCCTGGGCG AAACGCCCGT TAGTGAACGC 
TTCTACGACG AATCCTCGCA CTCCTCTTCC CACAGCGATG ATGGCAGTAG CGATGACGAA 
AAAAACAAGT CGAGTACGAA CAATAAAAAC AATGGCGGAG GAAATGGCAG TAGAACACCC 
AGTTCTCCGA GCAACAGCTA CCACATCGAT GTGGATCACC GCCTGCTGTT GAGGCAAACG 
AAACCATTGC TCCAATCGCG AAATGCCTCC GTGGTGATGG CAGTGGCGCA GCTCTATCAT 
CATGTGGCTC CCAAGAACGA AGTGCAATTG ATTGCCAAGG CGCTGATACG ACTGCTGCGA 
TCGCATAAGG AGGTTCAGAG CGTGGTCCTC AATTGCATTG CCTCGATGTC CACCAAACGA 
AAGGCGATCT TTGAGCCGCA CCTCAAGTCG TTCTTTGTGC GCACCAGCGA TCCCACGCAC 
ATAAAGCTTC TCAAGCTGGA TATTCTCACC AATCTGGCCT CGGCGGGCAG TATTTCGCTC 
ATTCTGCGCG AATTTCAAAC CTACATCTCC AGCAGCGATC GTTCGTTTGT GGCGGCCACG 
ATTCAGGCCA TTGGACGATG TGCATCTAGC ATTAAGGAGG TTACCGAGAC CTGCTTGAGT 
GGCCTGGTTC ATCTGCTGTC CAATCATGAC GAACACGTGG TGGCCGAGAG CGTGGTGGTG 
ATTAAGCGTC TTCTGCAAAC GAAAGCCGCC GAGCACTTTG AGATTATCAC CCAAATGGCC 
AAACTGATCG ATTATATCAA TGTGCCCGCA GCTAGGGCAG CCATCATTTG GCTTATAGGC 
GAATACAACG AGAAGGTGCC GCTGATTGCC CCGGATGTGC TGCGCAAAAT GGCCAAATCG 
TTTGTCGATG AGCAGGATGT GGTGAAGCTG CAGGTCCTAA ACTTAGGCGT TAAGCTCTAT 
CTGACCAATC CGGAGCAGAC ATCGTTGCTG TGTCAGTACG TCTTTACGCT GGCTCGCTAT 
GATCCCAACT ACGATGTACG CGATCGTGCC CGTTTTCTGC GGCAAATCAT CTTTCCGGCG 
AGCGGAACGA GCTCGGTGCT CAGCCAGCAT GCCCGCCAAG TATTTCTCGC CAGCAAACCG 
GCTCCGGTGC CGGAGAGTAA ATACCGGGAT GGCAATAACT TCCAGTTAGG ATCGTTGTCT 
CACTACCTGA ACATGCCAGC GGCTGGCTAC AAGGAACTGC CCGCCTTTCC GGTGATTCCA 
CCGGATTCAT CGGTACGCAA TATAGCTGGA TTTATGCAGG ACAAGCTGCC GGGTGAAGAC 
TCGCCATCCG GACATTCAAA GGATAAATCC GGCCGTGAAG GCGGTAAGGA AAAGGGTGCT 
GGTGGCGAGA AGGGCTTCCT CTCTGAGTCG GACGACAAGT CTTCCGCTTA CTCGGAATCA 
GGCAGCAGCA GTGGCAGTGG CACCAGCGAC AGTGAATCCG ATAGCGATGG CAGCGGGAGC 
AGCGATGAGG AGGAGGAGGA GGTGCAAAAG CAGCAGCCGA CCAAGGTTTC CTCGGTGAAG 
GAGCGGCTTA TCGATGCTGG AAGCAATGAG CTGGCCGCTA CTTCTTCGCC TGTAAAAGCA 
ACTATAAACA ACAATAACAA TGCGAGAGGT TCGTCAGGAA CATCGGACAG CGAGGATTCA 
TCAGCGTACA GTGGCAGTAG TAGTGATGAC AGCGCTTCCG GTAGCGATAA TGAAGTGAAA 
AAGAACGAAA CGAAAACGGA CACAGGCACA AAGCCGGAGA AGTTGAAAGA GAAGCACGAG 
AAACAGCCGG AGCAAACGGC TGTCAAGAGC AATCTGGACC TGCTACTCGA TCTGGATGAC 
ATTCCGCCCA TTGGTCCCGT GATGACGCCC TCGTTGGGAG GATTCCTGAC GCCTGGCACA 
CCAATGATGG CTGGACAGGC GGCGCCACTG CAGCCGCAGC ACGCCCGCAA TCGGGTGGAA 
TTGGTGGGTC CCTCGCACAT CGAGTTCAAG CACAAGGAGC TGCTTAACAA GGTCAGTGGC 
CATGGACTGC ATTTGGCCTA CCGTTTCACC CGCTCCCCGC ATCTGTACTC CTCGAGCATG 
TGCTCCATTG AGCTGCAGTT CCAGAATCGT GGCGAAAAAG AGATTACGGC CATTCGTCTC 
GGGCAAACAA CTCTGCCGGC GGGCATGCAA TTGAATGAGT TTGCGCCAAT CACTGTTCTC 
CAGCCCCAAC AAACAGCGAG TGGTGTGCTG GGCGTCGATT TCAATGATTC GACGCATGCT 
GTAGACCTGG AGCTTCTGTC AAGTGCGGGC AGTTCACGAC TGCAATTGAA ACCGCCGGTG 
GGCGAGCTGG TGAGATCCGT CCAGATCGGC GAGAGCTGTC ATCGCGAGGA GCGGGCCAAG 
CTGCGTGGAA TGAATGAGCA TCAGTGCGAG TTGCGTGGCC TGAGGCAGGA CCTAATCGAT 
GTGGTCGCAT TGCGGCAAAA GGTTTTCGAG TCCATCAACG TGGCGCATAC GCACAGCTCT 
TCCAGCGGTC AGCTACACTG TTTCGCCGGC CAAACCCTGA GCTCCAAAAG CCTCGTGCTA 
CTCACCCTCG ATTGGAAAAC AGAGGATGCC CTCACGCTGC TGGTAAACTG CGAGAAAATG 
GTCATTGGAT CCATGGTGCT CAACGAGCTG CGCAATGCTC TGCAGCTCAG TTTCGCCATG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
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Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
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The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
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Combined evidence annotation of transposable elements in genome sequences.
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rb cDNA ORF clone, Drosophila melanogaster(fruit fly)

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