NO66 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	NO66
Entrez Gene ID	31374
Full Name	CG2982 gene product from transcript CG2982-RB
Synonyms	Dmel\CG2982,anon-4Bd
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 4B6-4B6

mRNA and Protein(s)

mRNA	Protein	Name
NM_131932.4	NP_572160.1	nucleolar protein 66, isoform B
NM_167007.3	NP_726923.1	nucleolar protein 66, isoform C

Pathways from BioSystems

Homologies

	C7H14orf169	XP_001090820.1
Canis lupus familiaris (dog)	C8H14orf169	XP_005623685.1
Mus musculus (house mouse)	2410016O06Rik	NP_076122.2
Danio rerio (zebrafish)	zgc:162967	NP_001082857.1
Rattus norvegicus (Norway rat)	RGD1307704	NP_001101510.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001938	XP_321123.4
Bos taurus (cattle)	NO66	NP_001093172.1
Xenopus tropicalis (tropical clawed frog)	LOC100488246	XP_002933911.1
Gallus gallus (chicken)	C5H14ORF169	NP_001026367.1
Homo sapiens (human)	C14orf169	NP_078920.2
Drosophila melanogaster (fruit fly)	CG2982	NP_572160.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NO66 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NO66 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa00769	NM_131932.4 Latest version!	Drosophila melanogaster nucleolar protein 66, transcript variant B (NO66), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa00769	NM_167007.3 Latest version!	Drosophila melanogaster nucleolar protein 66, transcript variant C (NO66), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NO66 ( NM_131932.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_572160.1 Drosophila melanogaster nucleolar protein 66, transcript variant B (NO66), mRNA.

Price & Availability↑

CloneID	OFa00769
Clone ID Related Accession (Same CDS sequence)	NM_131932.4 , NM_167007.3
Accession Version	NM_131932.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nucleolar protein 66, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_131932.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAAAGG CGACTACTTC CGCGGCCGCC AAGTCTCAAG GCAACAGCAA AATGCAGAAA 
AACGCAAATA ACGGCACAGC TAAAGACAAG AAGAAGCCCA ATCTAGATAA AGAATCGAAT 
GATTCCAATT CGGTATCTGA TATGCTCGCC GTAACCAAGG ACCAAGAGGT GCATGCCTTT 
TTCTCTAAAT TATTCGACGA TGACGCCGGT CCATCAACAT CCAAAAAGAC CCAGTCAGGG 
TCAGCCGCAG CCGCTAAGAC CGCAGATCGC AAGCGACGTC TTCAGGCCGA GGCGGATGCC 
AACAATAATG ATACCGGAAA AGCCGGTAAG TTGACCAAGG AATCGGAAGC TACTCAAGGA 
GCCAGGGCCA CCAAACGCAA GCAAGCACGT AGTCTGGGAC TGGAGCGCAC CTCGCCGATC 
CAGGTTAATG GCGCGGCTTT AGCCTGCCCT TTGGTGCGCA AATCGCTGCC CCCAGGGGAG 
GCCAATTCCT GTCCTCAGCC GCCCAAAAAG GACCCAGCAG CCGTGAACTC ATTAGTTAAA 
ATAATTAAGG CGGAACCCAC CGAAGAGGGC AATAATAACA ATGACGAAAA GGAGACTGAA 
ACAATAGAAA CTCACAAAGC CGACAGCGTC GAAGAGGGTC GTCGCGTCCT GCAGTGGATA 
CTCTTTCCGG TCCAAACTAA AGTCTTCTTC AAAGACTTCT GGGAGCACAC CGCCTGCCTG 
GTGCAGCGCT CTAATCCCAA ATACTTTCAA AGTATGATAT CATTCAAGAT GCTTGACGAA 
ATACTGATTC GCCATCATTT GGACTTCACC GTCAACGTGG ATGTGACCAC TTACAAGAAC 
GGCAAGCGTG AGACACTGAA TCCCGAGGGT CGTGCCTTGC CACCAGCTGT GTGGGGCTTC 
TATTCGGACG GCTGCAGCAT TCGTTTACTG AATCCTTCCA CCTATCTAAT TCGCTTGCGT 
CAGGTGTGCA CCGTTTTGCA GGAGTTCTTT CACTGCAAGG TGGGGGCAAA TCTATACCTA 
ACCCCACCCA ATAGCCAGGG CTTTGCGCCC CATTACGATG ACATCGAGGC TTTCGTTATC 
CAAGTGGAGG GACGCAAGCG CTGGCTTTTG TACGAGCCGC CCAAGAAGGC CGATCAACTG 
GCCAGGATCT CGTCGGGCAA CTATGATCAA GAGCAGCTGG GTAAGCCCAT AATTGATGAG 
GTACTAAGCG CCGGCGATGT ACTATATTTC CCGAGAGGCG CTGTTCACCA GGCCATCACC 
GAAGAGCAAC AGCACTCGCT GCACATTACC TTGAGTGTTT ACCAACAGCA GGCATATGCC 
AATCTTCTAG AGACACTAAT GCCCATGGTT CTTAAGAAGG CCGTCGATCG GAGTGTGGCC 
TTGCGTCGCG GCTTGCCGCT GCACACTTTT CAAGTTTTGG GCAATGCCTA CAAGGGAAAT 
GACTGCGGGT CGCGCAAACA GCTGGTGGAG AATGTGCAGA AGCTGGTGAC CAATTACTTG 
ATGCCCTCCG AAGATGATAT TGATGAGGCC GTCGACCAGA TGGCCAAGAA GTTCCAGCAC 
GAGGCACTTC CGCCGATCGT ATTGCCCTCG GAGGAGGTGC GCACTGTTCA TGGGGCACGC 
AGTGATGCCG ATGAGCAGGG CAACTGCGTC TGCGATTACA AATTCAATAA AAAGACATCC 
GTGCGCCTGC TGCGTGCCAA CATTCTGCGT CTGGTGACCG AGTCCGATGG CAGCGTGCGT 
ATCTACCACC ATGTGGACAA TGGCCTGGAT TACTGCAAGT ACGAGCCCTA TTTCATGGAG 
ATTCTGCCAG AGGAAGCTAA AGCCGTTGAG CTCCTCATAT CGGCCTATCC ATTTTACCTC 
ACCATTGATC AGTTGCCATT GGAGTCTTCA GCTCGCAAGA TAGAGGTCGC CACCGCCCTG 
TGGGAGCATG GTCTTCTTAT GACCGAGAAG CCGTTTAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_572160.1
CDS	129..2090
Translation	MKKATTSAAAKSQGNSKMQKNANNGTAKDKKKPNLDKESNDSNSVSDMLAVTKDQEVHAFFSKLFDDDAGPSTSKKTQSGSAAAAKTADRKRRLQAEADANNNDTGKAGKLTKESEATQGARATKRKQARSLGLERTSPIQVNGAALACPLVRKSLPPGEANSCPQPPKKDPAAVNSLVKIIKAEPTEEGNNNNDEKETETIETHKADSVEEGRRVLQWILFPVQTKVFFKDFWEHTACLVQRSNPKYFQSMISFKMLDEILIRHHLDFTVNVDVTTYKNGKRETLNPEGRALPPAVWGFYSDGCSIRLLNPSTYLIRLRQVCTVLQEFFHCKVGANLYLTPPNSQGFAPHYDDIEAFVIQVEGRKRWLLYEPPKKADQLARISSGNYDQEQLGKPIIDEVLSAGDVLYFPRGAVHQAITEEQQHSLHITLSVYQQQAYANLLETLMPMVLKKAVDRSVALRRGLPLHTFQVLGNAYKGNDCGSRKQLVENVQKLVTNYLMPSEDDIDEAVDQMAKKFQHEALPPIVLPSEEVRTVHGARSDADEQGNCVCDYKFNKKTSVRLLRANILRLVTESDGSVRIYHHVDNGLDYCKYEPYFMEILPEEAKAVELLISAYPFYLTIDQLPLESSARKIEVATALWEHGLLMTEKPFK

Target ORF information:

RefSeq Version	NM_131932.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nucleolar protein 66, transcript variant B (NO66), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131932.4

ORF Insert Sequence:

ATGAAAAAGG CGACTACTTC CGCGGCCGCC AAGTCTCAAG GCAACAGCAA AATGCAGAAA 
AACGCAAATA ACGGCACAGC TAAAGACAAG AAGAAGCCCA ATCTAGATAA AGAATCGAAT 
GATTCCAATT CGGTATCTGA TATGCTCGCC GTAACCAAGG ACCAAGAGGT GCATGCCTTT 
TTCTCTAAAT TATTCGACGA TGACGCCGGT CCATCAACAT CCAAAAAGAC CCAGTCAGGG 
TCAGCCGCAG CCGCTAAGAC CGCAGATCGC AAGCGACGTC TTCAGGCCGA GGCGGATGCC 
AACAATAATG ATACCGGAAA AGCCGGTAAG TTGACCAAGG AATCGGAAGC TACTCAAGGA 
GCCAGGGCCA CCAAACGCAA GCAAGCACGT AGTCTGGGAC TGGAGCGCAC CTCGCCGATC 
CAGGTTAATG GCGCGGCTTT AGCCTGCCCT TTGGTGCGCA AATCGCTGCC CCCAGGGGAG 
GCCAATTCCT GTCCTCAGCC GCCCAAAAAG GACCCAGCAG CCGTGAACTC ATTAGTTAAA 
ATAATTAAGG CGGAACCCAC CGAAGAGGGC AATAATAACA ATGACGAAAA GGAGACTGAA 
ACAATAGAAA CTCACAAAGC CGACAGCGTC GAAGAGGGTC GTCGCGTCCT GCAGTGGATA 
CTCTTTCCGG TCCAAACTAA AGTCTTCTTC AAAGACTTCT GGGAGCACAC CGCCTGCCTG 
GTGCAGCGCT CTAATCCCAA ATACTTTCAA AGTATGATAT CATTCAAGAT GCTTGACGAA 
ATACTGATTC GCCATCATTT GGACTTCACC GTCAACGTGG ATGTGACCAC TTACAAGAAC 
GGCAAGCGTG AGACACTGAA TCCCGAGGGT CGTGCCTTGC CACCAGCTGT GTGGGGCTTC 
TATTCGGACG GCTGCAGCAT TCGTTTACTG AATCCTTCCA CCTATCTAAT TCGCTTGCGT 
CAGGTGTGCA CCGTTTTGCA GGAGTTCTTT CACTGCAAGG TGGGGGCAAA TCTATACCTA 
ACCCCACCCA ATAGCCAGGG CTTTGCGCCC CATTACGATG ACATCGAGGC TTTCGTTATC 
CAAGTGGAGG GACGCAAGCG CTGGCTTTTG TACGAGCCGC CCAAGAAGGC CGATCAACTG 
GCCAGGATCT CGTCGGGCAA CTATGATCAA GAGCAGCTGG GTAAGCCCAT AATTGATGAG 
GTACTAAGCG CCGGCGATGT ACTATATTTC CCGAGAGGCG CTGTTCACCA GGCCATCACC 
GAAGAGCAAC AGCACTCGCT GCACATTACC TTGAGTGTTT ACCAACAGCA GGCATATGCC 
AATCTTCTAG AGACACTAAT GCCCATGGTT CTTAAGAAGG CCGTCGATCG GAGTGTGGCC 
TTGCGTCGCG GCTTGCCGCT GCACACTTTT CAAGTTTTGG GCAATGCCTA CAAGGGAAAT 
GACTGCGGGT CGCGCAAACA GCTGGTGGAG AATGTGCAGA AGCTGGTGAC CAATTACTTG 
ATGCCCTCCG AAGATGATAT TGATGAGGCC GTCGACCAGA TGGCCAAGAA GTTCCAGCAC 
GAGGCACTTC CGCCGATCGT ATTGCCCTCG GAGGAGGTGC GCACTGTTCA TGGGGCACGC 
AGTGATGCCG ATGAGCAGGG CAACTGCGTC TGCGATTACA AATTCAATAA AAAGACATCC 
GTGCGCCTGC TGCGTGCCAA CATTCTGCGT CTGGTGACCG AGTCCGATGG CAGCGTGCGT 
ATCTACCACC ATGTGGACAA TGGCCTGGAT TACTGCAAGT ACGAGCCCTA TTTCATGGAG 
ATTCTGCCAG AGGAAGCTAA AGCCGTTGAG CTCCTCATAT CGGCCTATCC ATTTTACCTC 
ACCATTGATC AGTTGCCATT GGAGTCTTCA GCTCGCAAGA TAGAGGTCGC CACCGCCCTG 
TGGGAGCATG GTCTTCTTAT GACCGAGAAG CCGTTTAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NO66 ( NM_167007.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726923.1 Drosophila melanogaster nucleolar protein 66, transcript variant C (NO66), mRNA.

Price & Availability↑

CloneID	OFa00769
Clone ID Related Accession (Same CDS sequence)	NM_131932.4 , NM_167007.3
Accession Version	NM_167007.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	nucleolar protein 66, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167007.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAAAAGG CGACTACTTC CGCGGCCGCC AAGTCTCAAG GCAACAGCAA AATGCAGAAA 
AACGCAAATA ACGGCACAGC TAAAGACAAG AAGAAGCCCA ATCTAGATAA AGAATCGAAT 
GATTCCAATT CGGTATCTGA TATGCTCGCC GTAACCAAGG ACCAAGAGGT GCATGCCTTT 
TTCTCTAAAT TATTCGACGA TGACGCCGGT CCATCAACAT CCAAAAAGAC CCAGTCAGGG 
TCAGCCGCAG CCGCTAAGAC CGCAGATCGC AAGCGACGTC TTCAGGCCGA GGCGGATGCC 
AACAATAATG ATACCGGAAA AGCCGGTAAG TTGACCAAGG AATCGGAAGC TACTCAAGGA 
GCCAGGGCCA CCAAACGCAA GCAAGCACGT AGTCTGGGAC TGGAGCGCAC CTCGCCGATC 
CAGGTTAATG GCGCGGCTTT AGCCTGCCCT TTGGTGCGCA AATCGCTGCC CCCAGGGGAG 
GCCAATTCCT GTCCTCAGCC GCCCAAAAAG GACCCAGCAG CCGTGAACTC ATTAGTTAAA 
ATAATTAAGG CGGAACCCAC CGAAGAGGGC AATAATAACA ATGACGAAAA GGAGACTGAA 
ACAATAGAAA CTCACAAAGC CGACAGCGTC GAAGAGGGTC GTCGCGTCCT GCAGTGGATA 
CTCTTTCCGG TCCAAACTAA AGTCTTCTTC AAAGACTTCT GGGAGCACAC CGCCTGCCTG 
GTGCAGCGCT CTAATCCCAA ATACTTTCAA AGTATGATAT CATTCAAGAT GCTTGACGAA 
ATACTGATTC GCCATCATTT GGACTTCACC GTCAACGTGG ATGTGACCAC TTACAAGAAC 
GGCAAGCGTG AGACACTGAA TCCCGAGGGT CGTGCCTTGC CACCAGCTGT GTGGGGCTTC 
TATTCGGACG GCTGCAGCAT TCGTTTACTG AATCCTTCCA CCTATCTAAT TCGCTTGCGT 
CAGGTGTGCA CCGTTTTGCA GGAGTTCTTT CACTGCAAGG TGGGGGCAAA TCTATACCTA 
ACCCCACCCA ATAGCCAGGG CTTTGCGCCC CATTACGATG ACATCGAGGC TTTCGTTATC 
CAAGTGGAGG GACGCAAGCG CTGGCTTTTG TACGAGCCGC CCAAGAAGGC CGATCAACTG 
GCCAGGATCT CGTCGGGCAA CTATGATCAA GAGCAGCTGG GTAAGCCCAT AATTGATGAG 
GTACTAAGCG CCGGCGATGT ACTATATTTC CCGAGAGGCG CTGTTCACCA GGCCATCACC 
GAAGAGCAAC AGCACTCGCT GCACATTACC TTGAGTGTTT ACCAACAGCA GGCATATGCC 
AATCTTCTAG AGACACTAAT GCCCATGGTT CTTAAGAAGG CCGTCGATCG GAGTGTGGCC 
TTGCGTCGCG GCTTGCCGCT GCACACTTTT CAAGTTTTGG GCAATGCCTA CAAGGGAAAT 
GACTGCGGGT CGCGCAAACA GCTGGTGGAG AATGTGCAGA AGCTGGTGAC CAATTACTTG 
ATGCCCTCCG AAGATGATAT TGATGAGGCC GTCGACCAGA TGGCCAAGAA GTTCCAGCAC 
GAGGCACTTC CGCCGATCGT ATTGCCCTCG GAGGAGGTGC GCACTGTTCA TGGGGCACGC 
AGTGATGCCG ATGAGCAGGG CAACTGCGTC TGCGATTACA AATTCAATAA AAAGACATCC 
GTGCGCCTGC TGCGTGCCAA CATTCTGCGT CTGGTGACCG AGTCCGATGG CAGCGTGCGT 
ATCTACCACC ATGTGGACAA TGGCCTGGAT TACTGCAAGT ACGAGCCCTA TTTCATGGAG 
ATTCTGCCAG AGGAAGCTAA AGCCGTTGAG CTCCTCATAT CGGCCTATCC ATTTTACCTC 
ACCATTGATC AGTTGCCATT GGAGTCTTCA GCTCGCAAGA TAGAGGTCGC CACCGCCCTG 
TGGGAGCATG GTCTTCTTAT GACCGAGAAG CCGTTTAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726923.1
CDS	125..2086
Translation	MKKATTSAAAKSQGNSKMQKNANNGTAKDKKKPNLDKESNDSNSVSDMLAVTKDQEVHAFFSKLFDDDAGPSTSKKTQSGSAAAAKTADRKRRLQAEADANNNDTGKAGKLTKESEATQGARATKRKQARSLGLERTSPIQVNGAALACPLVRKSLPPGEANSCPQPPKKDPAAVNSLVKIIKAEPTEEGNNNNDEKETETIETHKADSVEEGRRVLQWILFPVQTKVFFKDFWEHTACLVQRSNPKYFQSMISFKMLDEILIRHHLDFTVNVDVTTYKNGKRETLNPEGRALPPAVWGFYSDGCSIRLLNPSTYLIRLRQVCTVLQEFFHCKVGANLYLTPPNSQGFAPHYDDIEAFVIQVEGRKRWLLYEPPKKADQLARISSGNYDQEQLGKPIIDEVLSAGDVLYFPRGAVHQAITEEQQHSLHITLSVYQQQAYANLLETLMPMVLKKAVDRSVALRRGLPLHTFQVLGNAYKGNDCGSRKQLVENVQKLVTNYLMPSEDDIDEAVDQMAKKFQHEALPPIVLPSEEVRTVHGARSDADEQGNCVCDYKFNKKTSVRLLRANILRLVTESDGSVRIYHHVDNGLDYCKYEPYFMEILPEEAKAVELLISAYPFYLTIDQLPLESSARKIEVATALWEHGLLMTEKPFK

Target ORF information:

RefSeq Version	NM_167007.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster nucleolar protein 66, transcript variant C (NO66), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167007.3

ORF Insert Sequence:

ATGAAAAAGG CGACTACTTC CGCGGCCGCC AAGTCTCAAG GCAACAGCAA AATGCAGAAA 
AACGCAAATA ACGGCACAGC TAAAGACAAG AAGAAGCCCA ATCTAGATAA AGAATCGAAT 
GATTCCAATT CGGTATCTGA TATGCTCGCC GTAACCAAGG ACCAAGAGGT GCATGCCTTT 
TTCTCTAAAT TATTCGACGA TGACGCCGGT CCATCAACAT CCAAAAAGAC CCAGTCAGGG 
TCAGCCGCAG CCGCTAAGAC CGCAGATCGC AAGCGACGTC TTCAGGCCGA GGCGGATGCC 
AACAATAATG ATACCGGAAA AGCCGGTAAG TTGACCAAGG AATCGGAAGC TACTCAAGGA 
GCCAGGGCCA CCAAACGCAA GCAAGCACGT AGTCTGGGAC TGGAGCGCAC CTCGCCGATC 
CAGGTTAATG GCGCGGCTTT AGCCTGCCCT TTGGTGCGCA AATCGCTGCC CCCAGGGGAG 
GCCAATTCCT GTCCTCAGCC GCCCAAAAAG GACCCAGCAG CCGTGAACTC ATTAGTTAAA 
ATAATTAAGG CGGAACCCAC CGAAGAGGGC AATAATAACA ATGACGAAAA GGAGACTGAA 
ACAATAGAAA CTCACAAAGC CGACAGCGTC GAAGAGGGTC GTCGCGTCCT GCAGTGGATA 
CTCTTTCCGG TCCAAACTAA AGTCTTCTTC AAAGACTTCT GGGAGCACAC CGCCTGCCTG 
GTGCAGCGCT CTAATCCCAA ATACTTTCAA AGTATGATAT CATTCAAGAT GCTTGACGAA 
ATACTGATTC GCCATCATTT GGACTTCACC GTCAACGTGG ATGTGACCAC TTACAAGAAC 
GGCAAGCGTG AGACACTGAA TCCCGAGGGT CGTGCCTTGC CACCAGCTGT GTGGGGCTTC 
TATTCGGACG GCTGCAGCAT TCGTTTACTG AATCCTTCCA CCTATCTAAT TCGCTTGCGT 
CAGGTGTGCA CCGTTTTGCA GGAGTTCTTT CACTGCAAGG TGGGGGCAAA TCTATACCTA 
ACCCCACCCA ATAGCCAGGG CTTTGCGCCC CATTACGATG ACATCGAGGC TTTCGTTATC 
CAAGTGGAGG GACGCAAGCG CTGGCTTTTG TACGAGCCGC CCAAGAAGGC CGATCAACTG 
GCCAGGATCT CGTCGGGCAA CTATGATCAA GAGCAGCTGG GTAAGCCCAT AATTGATGAG 
GTACTAAGCG CCGGCGATGT ACTATATTTC CCGAGAGGCG CTGTTCACCA GGCCATCACC 
GAAGAGCAAC AGCACTCGCT GCACATTACC TTGAGTGTTT ACCAACAGCA GGCATATGCC 
AATCTTCTAG AGACACTAAT GCCCATGGTT CTTAAGAAGG CCGTCGATCG GAGTGTGGCC 
TTGCGTCGCG GCTTGCCGCT GCACACTTTT CAAGTTTTGG GCAATGCCTA CAAGGGAAAT 
GACTGCGGGT CGCGCAAACA GCTGGTGGAG AATGTGCAGA AGCTGGTGAC CAATTACTTG 
ATGCCCTCCG AAGATGATAT TGATGAGGCC GTCGACCAGA TGGCCAAGAA GTTCCAGCAC 
GAGGCACTTC CGCCGATCGT ATTGCCCTCG GAGGAGGTGC GCACTGTTCA TGGGGCACGC 
AGTGATGCCG ATGAGCAGGG CAACTGCGTC TGCGATTACA AATTCAATAA AAAGACATCC 
GTGCGCCTGC TGCGTGCCAA CATTCTGCGT CTGGTGACCG AGTCCGATGG CAGCGTGCGT 
ATCTACCACC ATGTGGACAA TGGCCTGGAT TACTGCAAGT ACGAGCCCTA TTTCATGGAG 
ATTCTGCCAG AGGAAGCTAA AGCCGTTGAG CTCCTCATAT CGGCCTATCC ATTTTACCTC 
ACCATTGATC AGTTGCCATT GGAGTCTTCA GCTCGCAAGA TAGAGGTCGC CACCGCCCTG 
TGGGAGCATG GTCTTCTTAT GACCGAGAAG CCGTTTAAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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NO66 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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