ec cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	ec
Entrez Gene ID	31339
Full Name	echinus
Synonyms	CG2904,Dmel\CG2904,EG:EG0002.3,Ec,Q1W9Q0
General protein information	Preferred Names echinus Names CG2904 gene product from transcript CG2904-RB CG2904-PA CG2904-PB CG2904-PC ec-PA ec-PB ec-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 3F2-3F3\|1-5.5 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001103407.3	NP_001096877.1	echinus, isoform C
NM_166992.3	NP_726893.2	echinus, isoform A
NM_130722.4	NP_570078.2	echinus, isoform B

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000205	XP_566191.4
Drosophila melanogaster (fruit fly)	ec	NP_570078.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

a new allele of echinus was isolated; findings show that echinus displays defects in the rotation of ommatidia that are not evident until mid-pupal stages, and we propose that echinus action is that of opposing EGFR
Title: A new allele uncovers the role of echinus in the control of ommatidial rotation in the Drosophila eye.

The echinus locus encodes multiple splice forms of a protein with homology to ubiquitin-specific proteases, but protease activity is unlikely to be required for echinus function, at least when echinus is overexpressed.
Title: echinus, required for interommatidial cell sorting and cell death in the Drosophila pupal retina, encodes a protein with homology to ubiquitin-specific proteases.

The following ec gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ec cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa00727	NM_130722.4 Latest version!	Drosophila melanogaster echinus (ec), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OFa00728	NM_166992.3 Latest version!	Drosophila melanogaster echinus (ec), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OFa00729	NM_001103407.3 Latest version!	Drosophila melanogaster echinus (ec), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ec ( NM_130722.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_570078.2 Drosophila melanogaster echinus (ec), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa00727
Clone ID Related Accession (Same CDS sequence)	NM_130722.4
Accession Version	NM_130722.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	echinus, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_130722.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCAGCCC ACAGTGGACG CAGAATGACC ACGCCGGGCA CACTTTATCG GTTGGCCAGT 
GGCTCAGCTG CCACACCAGC CACCCCCACA AACAGCCCTT ATGGGCAGCC GGCAAAGCCA 
TATGCGGTGG GTTCAATCCA GAACGGATCG GAGCGGAATC GCATTCTGGA GTTGCTGAGA 
GCACCCAATA GCTTGACCTC GCCCACGCTG GAGCCCTCCT CTAAGGGACT ACTCAATGGA 
CCCGGGCAGA ACAACTGCTT TCTCAATTGT GCCGTACAGG TCTTGTGGCA CTTGGACGCC 
TTTCGACGAT CGTTTCGCGG CCTAAATCAG CACGTATGCG GTGGCCAAGA CTGTATCTTT 
TGCGCTCTAA AGGAACTTTT CCAGCAGCTG CAGACCAGTT CTGAGCCCGC CTTGTGCCCG 
GAGCCACTGC GTCGGGCTTT GGCCAGTGGT CCATTGGCTG GACGGCGATT CCCACTCGGC 
TGCTTGGGCG ATGCAGCCGA ATGCTTCGAG CTCCTTCTCC ACCGCGTCCA CTCGCACATT 
TCGCCGGATG ACGGCGACTC CTGCGAATCC TCCGCCTGCA TTGCCCACCG AAGATTCGCC 
ATGCGCGTCA TCGAGCAGAG CGTCTGCAAG TGCGGAGCCA ATTCGGAGCA GCTGCCCTTC 
ACCCAGATGG TGCACTACGT CTCTGCCTCG GCTCTGACCT CCCAGAAAAG TCTGGCACTG 
CAGTCCCATC AGCAACTGAG TTTTGGCCAG CTTTTAAGGG CCGCCGGCAA CATGGGCGAC 
ATACGCGATT GTCCGAACAC CTGCGGCGCC AAGATTGGAA TTTGTCGTGC GCTGCTTAAC 
CGACCAGAAG TGGTGTCCAT TGGCATCGTT TGGGACTCTG AGCGACCGGC GGCGGATCAG 
GTTCATGCCG TCCTAAAGGC AGTGGGCACC AGCCTGCGTC TGGGTGACGT TTTTCACCAG 
GTCAGCGAGC CACGATGGGC CCAGCAGACC CAGCACGAGC TGGTTGGCAT CGTTTCCTAC 
TACGGCAAAC ACTACACCAC CTTCTTCTTC CACACGAAGC TCAAGGTGTG GGTCTACTTC 
GATGATGCGA ACGTTAAGGA GGTGGGGCCC AGTTGGGAGG GCGTGGTGGA CAAATGCAGC 
CGGGGAAGAT ATCAGCCACT CCTGCTGCTG TACGCCGTTC CCCAGCAGCC TACGGTGGGC 
AACGGCGTAG GTATCGGTCA CGTGGATGTG GCCATGCCGG CGAGTGCTCC ATCGATAGCT 
GGCTCAGTGG TTCGCAGGGC AGTTACGCCC AGTCCGGAGA AGCCCAGCTT GGGCAACACA 
AGGAGGGCCA TCACCCCCAC TCCGCTGAGG ACACCACCCA ACGACTATCA GAACCTGAGT 
GTGATTCAAA AGAATATCTT TCCGAGCAAT CTGGCAATGG GTACGGATGA AACGGATGCC 
TACATTAGCC GCAAGACGGT TGAACATGTG CTGAGTGCCC AGTACCAGAA TTTGAGCGTA 
ATCCAGGACA AGATTGGAGG CGGCAATGGA GTCGGTGTCG GCCAACAGAG TGTGGATAAG 
GATGGAGGCT TTCTGAGCCG AAAGCCCATT GAGGCAGTGC TGAGTGCCCA ACTGGCGCGG 
AGGCAGCACC TCCAGCTTCA ACGCAGCCAC AGTGCGGAGT CAAGCTCCGG CCACGCCTCC 
TCCAACGGCA GTTCTCCGCC CAGCGATGGA CTGACCATGC CGGAGCACCT GAATCAGCCA 
CGTCGTCGCG ACTCGGGCAA TTGGTCAGGC GATCGAAACA GCGCTAGTTC GGCCAGTTCC 
ACGACGCTGG ATAATCCCTA CCTGTACATG GTGGCCAAGC GGGGTGTGGC AGCAGTGCCA 
CAGAGCCCCA CGCGACATGG TTTGCCATAT GACCCGGGCT ACGATTCGTT TTCGCTAAGC 
TCCACGGACT CATATCCGCC GAAGCATGCG CTAAATCCAC AGCTGGCTAA AATCCCGGAA 
GCAGCCATGG GCGTCGTTCC AAACGGAGGA GCAATGCCCC ATGGACATGG CAACGGACAG 
CTGGCCTTGT CTGGGGACTG CGAGAAGCTG TGCCACGAGG CGGACCAGTT GCTGGAAAAG 
TCCCGACTGG TGGAGGAGTC ACATGATCTG GAAACAGCCC TGGTGCTCTG CAATGCGGCT 
GCTGGGCGGG CACGAGCCGC TATGGATGCC CCGTATAGCA ATCCGCACAC GATGACCTTT 
GCCCGGATGA AGCACAATAC TTGCGTGATG CGGGCCCGTA GTCTGCACCG GCGGATTCTG 
GTGGAGAAGG GAGCTGAAAC AGAGGCTATG CCCGAACTGA AGCACATGCG CGAAGGAAGC 
ACGAGTAGTG TGAAGCATGT GCGCCAGAAT AGCAAGGATC GCACGTTAGA GAAGGAGCAG 
CAGCAGCTGC AGTTCCAGCA AATACAGCAG ATCCAGCAGA TTCATCAGAT CCAGCAGCAG 
ATCCCAGCCA GCATCGAAAT ATATGCCACG CTCCCGAAGC GCAAGAGTCC TCTGAAGGCC 
TTGGCCTCTG CGTCTGCGTG CGATGACAAC GCAATCGAGT ACGAGCAGGA GAAGCAACTG 
GCCACCAGTC CGGGAAAACC GGAGCGCGAG AGTCGCTCGC TGTTCGGGCG CAAGGACAAA 
GAGAAGGAGA AGCGGAGTCG CAGCGAGGAT CGCAACAAGC TAACCCGTGA GTTCTCGCTC 
ACCGAAACGC TTCTAGTGAA TGCCAAGGAT ACGCTGAAGA AGCATAAGGA GGACAAGGAC 
GAGAAGAAGG AGAAGGACAA GAGCGGCAAA AAGCAGCACA AAATCAGACG CAAGCTGTTA 
ATGGGCGGGC TCATTCGTCG CAAGAATCGC TCCATGCCGG ATCTCACAGA GGCGGTGGAT 
GACTCGACGG CCAATGCCGG CATAACGCAC CATCCACATT ATCCTGCACA GGGCGTAACT 
ACGGCGGGCA CTCTCTTGGG TACCTCCGTA GACGACAGTG CCGTTGGATT GGGCAAACAC 
GGCCACGGCA TGAGCGGCTA CATCTCTGAG GGACACTTCG ACTACTCGGG TTCCGGGTCG 
GCCTCAAATT CGGGAGCCGG CAGCAATCCA AATCCGAATC TGGAGAGGAG CAAGCTGATG 
CGCAAGAGCT TCCACGGCAG CGGCAGGCAG TTGACAATGG TACCGAAAGT GGCTCCACCG 
CCGCCCATGC GAACCAGCTC CACTTTGACC CCACAGCAGC AACAACAACA ACAACAGCAA 
TTTCACCATG AGGCCAACTT GTCCAACCTC TCGGCCATGA GTTCAAACAC CTCGATTAGC 
GAGGATTCCT GCCAGACGAT CATCACCACT TGCGCACAGG TGCACTCGGA GCAGAGTCCA 
CTAAAGGATC TGCAAATGCT GGTCGCTCAG GACGAGCTGC CACTGCCGCC GCCAATGGAA 
CTTCCTCCGT ACCCAAGTCC ACCGCACTCC TCCTGCCATT CGCGGCAGGC TAGCGAAGAT 
TTCCCGCCGC CACCACCGCC GGAACTGGAT CTGGAGCCAC TCAACCAGCA ACTGAGCCAG 
CTGCAGGCAC TGGAGGCGGC CAGTAAGCAG CAGCGCCAGC AACTCGAGTC CCTCGAGGGC 
ACCACCAGCA TTTTGGCCCA GCTGCAGCAG CGCCAGCACC TGCTCAAGCT GAGAAAGGAG 
CAGGCCAATC ACCCGGGAGC AGCCACCACG GATGCCACCT GGCTGAAGGA GCTGCAGGCG 
AAACAGGCAA ACGATTTGAG AGCCATGCAA CGTAAGCTGG AGGCCTCGTC GCCATCAAGT 
GTCAGGGACT TGACTCATCG TTTCGAGCAA ACCTCCATTC GATCCTACGC CTCGCAGGAG 
CTATTGTCCC AGCCGGGACA GAGTCAGCAG CTGGGGCAGC TGCGCACCCA GATGAATGGG 
CTGCCAAACG GCAATGCAAA AATGGATGTA GATGAGGTGG ACGCTATGCC AGCGGTGAGA 
AACTCTCTGC CCGCCGCACT CAGCGCCCAC CAATTGCTGC CCAAGCCTAA GTACGAAATG 
ACCCAATCGC AGATTGCAGA GGAGATTCGC GAAGTGGAGT TGCTGAACAC GATGGTACAG 
CAGACACTGA ATCAGAACGG AGCTGCCGCA CTACCTAAGC GGGTAAAGAA AAAGAGCGTC 
TCCTTTTGCG ACCAGGTGAT CCTGGTTGCC ACAGCCGGAA ACGAGGAGGA CGATGACTTT 
ATACCCAATC CCATACTGGA GCGGGTCCTG CGGACTGCCC AGCATCCCAA CGAAAAAGTC 
ACTGCTCAAA TGATCCAGCA GCAGCAGCAG AACATGATGC GATTGCAAAC AGAAGCGCAG 
CCGCGGCAAC AGCAGGTGCA GCAGCTGCAA CAACCATCAC CCATGTTGGG CAGACCAGCA 
GGAGCCACAG CAGATATGCA GCGATACGTC CAGAGGATGC AGCAACAACA GCAGCAGCAG 
CAACAACAGC AGCAGCAACA ACAGCAGCAG CAGCAACAAC AGCAGCAACA ACAGCAGCAG 
CAACAGCAGC AGCAGCAGCA ACACCAGGAG TTGTACCGCC AGCAGCAGCT ACATCGCACT 
AGTATGAGCG GCATAGCCAG CCAGCAACAC TTGCAGCAAT TGGATGCACA GTACACCACC 
ATGCCGCGTC CCTTGCATCA TTCACAGCTT GGTAGCTACT CTGTGACCTT GCAGAAACAC 
CAGCAACTTC AGCAGCAACA GCAACAGCAG CAACAGCAGC AGGCCCCGCA GCAGCTCAGC 
TTGGGCTACT TTAGCAATGG CAGTGTTGCG ACTTCAGAGC AAGTCCCGCT GCCTTCGCCC 
TACCAGCGGG TGCCCCTGCC ACATGGATAC CAGCCTGCAG CAGCAGGACC CTACTACCCG 
CTACCCAATG CCCAGCAACA TCTGCAGCAG CAACAGCTGA TGGCTAAGCC CGTGCAGAAA 
AAGGTGAGCT TTGAGCCAGG AACTAAAGGC GAAGCCGATT GCTTGCCCCC ACCACCGCCG 
CCGCCGCAAA AGAACGGCTT GCCCGAGACG GGATCTGCAG GATCAGGACA CCCTGGAGCA 
GCACCCGGGG CAAGTGGAGC GGGCGGCACC ACATCGGCCG GCATCACCGC CATTCCCACC 
CGCGTCTACA ACAATGCCAT TGTAAAGGCT TCGGCCAAAG CCGTTGAGTG CAATTTGTGC 
CGCAAGCGGC ATGTGATCGC ACCGGCGGTA TATTGCACCA ACTGCGAGTA CTACCTGCAG 
ATGCTGAACC AGCGCAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_570078.2
CDS	587..5827
Translation	MSAHSGRRMTTPGTLYRLASGSAATPATPTNSPYGQPAKPYAVGSIQNGSERNRILELLRAPNSLTSPTLEPSSKGLLNGPGQNNCFLNCAVQVLWHLDAFRRSFRGLNQHVCGGQDCIFCALKELFQQLQTSSEPALCPEPLRRALASGPLAGRRFPLGCLGDAAECFELLLHRVHSHISPDDGDSCESSACIAHRRFAMRVIEQSVCKCGANSEQLPFTQMVHYVSASALTSQKSLALQSHQQLSFGQLLRAAGNMGDIRDCPNTCGAKIGICRALLNRPEVVSIGIVWDSERPAADQVHAVLKAVGTSLRLGDVFHQVSEPRWAQQTQHELVGIVSYYGKHYTTFFFHTKLKVWVYFDDANVKEVGPSWEGVVDKCSRGRYQPLLLLYAVPQQPTVGNGVGIGHVDVAMPASAPSIAGSVVRRAVTPSPEKPSLGNTRRAITPTPLRTPPNDYQNLSVIQKNIFPSNLAMGTDETDAYISRKTVEHVLSAQYQNLSVIQDKIGGGNGVGVGQQSVDKDGGFLSRKPIEAVLSAQLARRQHLQLQRSHSAESSSGHASSNGSSPPSDGLTMPEHLNQPRRRDSGNWSGDRNSASSASSTTLDNPYLYMVAKRGVAAVPQSPTRHGLPYDPGYDSFSLSSTDSYPPKHALNPQLAKIPEAAMGVVPNGGAMPHGHGNGQLALSGDCEKLCHEADQLLEKSRLVEESHDLETALVLCNAAAGRARAAMDAPYSNPHTMTFARMKHNTCVMRARSLHRRILVEKGAETEAMPELKHMREGSTSSVKHVRQNSKDRTLEKEQQQLQFQQIQQIQQIHQIQQQIPASIEIYATLPKRKSPLKALASASACDDNAIEYEQEKQLATSPGKPERESRSLFGRKDKEKEKRSRSEDRNKLTREFSLTETLLVNAKDTLKKHKEDKDEKKEKDKSGKKQHKIRRKLLMGGLIRRKNRSMPDLTEAVDDSTANAGITHHPHYPAQGVTTAGTLLGTSVDDSAVGLGKHGHGMSGYISEGHFDYSGSGSASNSGAGSNPNPNLERSKLMRKSFHGSGRQLTMVPKVAPPPPMRTSSTLTPQQQQQQQQQFHHEANLSNLSAMSSNTSISEDSCQTIITTCAQVHSEQSPLKDLQMLVAQDELPLPPPMELPPYPSPPHSSCHSRQASEDFPPPPPPELDLEPLNQQLSQLQALEAASKQQRQQLESLEGTTSILAQLQQRQHLLKLRKEQANHPGAATTDATWLKELQAKQANDLRAMQRKLEASSPSSVRDLTHRFEQTSIRSYASQELLSQPGQSQQLGQLRTQMNGLPNGNAKMDVDEVDAMPAVRNSLPAALSAHQLLPKPKYEMTQSQIAEEIREVELLNTMVQQTLNQNGAAALPKRVKKKSVSFCDQVILVATAGNEEDDDFIPNPILERVLRTAQHPNEKVTAQMIQQQQQNMMRLQTEAQPRQQQVQQLQQPSPMLGRPAGATADMQRYVQRMQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQHQELYRQQQLHRTSMSGIASQQHLQQLDAQYTTMPRPLHHSQLGSYSVTLQKHQQLQQQQQQQQQQQAPQQLSLGYFSNGSVATSEQVPLPSPYQRVPLPHGYQPAAAGPYYPLPNAQQHLQQQQLMAKPVQKKVSFEPGTKGEADCLPPPPPPPQKNGLPETGSAGSGHPGAAPGASGAGGTTSAGITAIPTRVYNNAIVKASAKAVECNLCRKRHVIAPAVYCTNCEYYLQMLNQRR

Target ORF information:

RefSeq Version	NM_130722.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster echinus (ec), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130722.4

ORF Insert Sequence:

ATGTCAGCCC ACAGTGGACG CAGAATGACC ACGCCGGGCA CACTTTATCG GTTGGCCAGT 
GGCTCAGCTG CCACACCAGC CACCCCCACA AACAGCCCTT ATGGGCAGCC GGCAAAGCCA 
TATGCGGTGG GTTCAATCCA GAACGGATCG GAGCGGAATC GCATTCTGGA GTTGCTGAGA 
GCACCCAATA GCTTGACCTC GCCCACGCTG GAGCCCTCCT CTAAGGGACT ACTCAATGGA 
CCCGGGCAGA ACAACTGCTT TCTCAATTGT GCCGTACAGG TCTTGTGGCA CTTGGACGCC 
TTTCGACGAT CGTTTCGCGG CCTAAATCAG CACGTATGCG GTGGCCAAGA CTGTATCTTT 
TGCGCTCTAA AGGAACTTTT CCAGCAGCTG CAGACCAGTT CTGAGCCCGC CTTGTGCCCG 
GAGCCACTGC GTCGGGCTTT GGCCAGTGGT CCATTGGCTG GACGGCGATT CCCACTCGGC 
TGCTTGGGCG ATGCAGCCGA ATGCTTCGAG CTCCTTCTCC ACCGCGTCCA CTCGCACATT 
TCGCCGGATG ACGGCGACTC CTGCGAATCC TCCGCCTGCA TTGCCCACCG AAGATTCGCC 
ATGCGCGTCA TCGAGCAGAG CGTCTGCAAG TGCGGAGCCA ATTCGGAGCA GCTGCCCTTC 
ACCCAGATGG TGCACTACGT CTCTGCCTCG GCTCTGACCT CCCAGAAAAG TCTGGCACTG 
CAGTCCCATC AGCAACTGAG TTTTGGCCAG CTTTTAAGGG CCGCCGGCAA CATGGGCGAC 
ATACGCGATT GTCCGAACAC CTGCGGCGCC AAGATTGGAA TTTGTCGTGC GCTGCTTAAC 
CGACCAGAAG TGGTGTCCAT TGGCATCGTT TGGGACTCTG AGCGACCGGC GGCGGATCAG 
GTTCATGCCG TCCTAAAGGC AGTGGGCACC AGCCTGCGTC TGGGTGACGT TTTTCACCAG 
GTCAGCGAGC CACGATGGGC CCAGCAGACC CAGCACGAGC TGGTTGGCAT CGTTTCCTAC 
TACGGCAAAC ACTACACCAC CTTCTTCTTC CACACGAAGC TCAAGGTGTG GGTCTACTTC 
GATGATGCGA ACGTTAAGGA GGTGGGGCCC AGTTGGGAGG GCGTGGTGGA CAAATGCAGC 
CGGGGAAGAT ATCAGCCACT CCTGCTGCTG TACGCCGTTC CCCAGCAGCC TACGGTGGGC 
AACGGCGTAG GTATCGGTCA CGTGGATGTG GCCATGCCGG CGAGTGCTCC ATCGATAGCT 
GGCTCAGTGG TTCGCAGGGC AGTTACGCCC AGTCCGGAGA AGCCCAGCTT GGGCAACACA 
AGGAGGGCCA TCACCCCCAC TCCGCTGAGG ACACCACCCA ACGACTATCA GAACCTGAGT 
GTGATTCAAA AGAATATCTT TCCGAGCAAT CTGGCAATGG GTACGGATGA AACGGATGCC 
TACATTAGCC GCAAGACGGT TGAACATGTG CTGAGTGCCC AGTACCAGAA TTTGAGCGTA 
ATCCAGGACA AGATTGGAGG CGGCAATGGA GTCGGTGTCG GCCAACAGAG TGTGGATAAG 
GATGGAGGCT TTCTGAGCCG AAAGCCCATT GAGGCAGTGC TGAGTGCCCA ACTGGCGCGG 
AGGCAGCACC TCCAGCTTCA ACGCAGCCAC AGTGCGGAGT CAAGCTCCGG CCACGCCTCC 
TCCAACGGCA GTTCTCCGCC CAGCGATGGA CTGACCATGC CGGAGCACCT GAATCAGCCA 
CGTCGTCGCG ACTCGGGCAA TTGGTCAGGC GATCGAAACA GCGCTAGTTC GGCCAGTTCC 
ACGACGCTGG ATAATCCCTA CCTGTACATG GTGGCCAAGC GGGGTGTGGC AGCAGTGCCA 
CAGAGCCCCA CGCGACATGG TTTGCCATAT GACCCGGGCT ACGATTCGTT TTCGCTAAGC 
TCCACGGACT CATATCCGCC GAAGCATGCG CTAAATCCAC AGCTGGCTAA AATCCCGGAA 
GCAGCCATGG GCGTCGTTCC AAACGGAGGA GCAATGCCCC ATGGACATGG CAACGGACAG 
CTGGCCTTGT CTGGGGACTG CGAGAAGCTG TGCCACGAGG CGGACCAGTT GCTGGAAAAG 
TCCCGACTGG TGGAGGAGTC ACATGATCTG GAAACAGCCC TGGTGCTCTG CAATGCGGCT 
GCTGGGCGGG CACGAGCCGC TATGGATGCC CCGTATAGCA ATCCGCACAC GATGACCTTT 
GCCCGGATGA AGCACAATAC TTGCGTGATG CGGGCCCGTA GTCTGCACCG GCGGATTCTG 
GTGGAGAAGG GAGCTGAAAC AGAGGCTATG CCCGAACTGA AGCACATGCG CGAAGGAAGC 
ACGAGTAGTG TGAAGCATGT GCGCCAGAAT AGCAAGGATC GCACGTTAGA GAAGGAGCAG 
CAGCAGCTGC AGTTCCAGCA AATACAGCAG ATCCAGCAGA TTCATCAGAT CCAGCAGCAG 
ATCCCAGCCA GCATCGAAAT ATATGCCACG CTCCCGAAGC GCAAGAGTCC TCTGAAGGCC 
TTGGCCTCTG CGTCTGCGTG CGATGACAAC GCAATCGAGT ACGAGCAGGA GAAGCAACTG 
GCCACCAGTC CGGGAAAACC GGAGCGCGAG AGTCGCTCGC TGTTCGGGCG CAAGGACAAA 
GAGAAGGAGA AGCGGAGTCG CAGCGAGGAT CGCAACAAGC TAACCCGTGA GTTCTCGCTC 
ACCGAAACGC TTCTAGTGAA TGCCAAGGAT ACGCTGAAGA AGCATAAGGA GGACAAGGAC 
GAGAAGAAGG AGAAGGACAA GAGCGGCAAA AAGCAGCACA AAATCAGACG CAAGCTGTTA 
ATGGGCGGGC TCATTCGTCG CAAGAATCGC TCCATGCCGG ATCTCACAGA GGCGGTGGAT 
GACTCGACGG CCAATGCCGG CATAACGCAC CATCCACATT ATCCTGCACA GGGCGTAACT 
ACGGCGGGCA CTCTCTTGGG TACCTCCGTA GACGACAGTG CCGTTGGATT GGGCAAACAC 
GGCCACGGCA TGAGCGGCTA CATCTCTGAG GGACACTTCG ACTACTCGGG TTCCGGGTCG 
GCCTCAAATT CGGGAGCCGG CAGCAATCCA AATCCGAATC TGGAGAGGAG CAAGCTGATG 
CGCAAGAGCT TCCACGGCAG CGGCAGGCAG TTGACAATGG TACCGAAAGT GGCTCCACCG 
CCGCCCATGC GAACCAGCTC CACTTTGACC CCACAGCAGC AACAACAACA ACAACAGCAA 
TTTCACCATG AGGCCAACTT GTCCAACCTC TCGGCCATGA GTTCAAACAC CTCGATTAGC 
GAGGATTCCT GCCAGACGAT CATCACCACT TGCGCACAGG TGCACTCGGA GCAGAGTCCA 
CTAAAGGATC TGCAAATGCT GGTCGCTCAG GACGAGCTGC CACTGCCGCC GCCAATGGAA 
CTTCCTCCGT ACCCAAGTCC ACCGCACTCC TCCTGCCATT CGCGGCAGGC TAGCGAAGAT 
TTCCCGCCGC CACCACCGCC GGAACTGGAT CTGGAGCCAC TCAACCAGCA ACTGAGCCAG 
CTGCAGGCAC TGGAGGCGGC CAGTAAGCAG CAGCGCCAGC AACTCGAGTC CCTCGAGGGC 
ACCACCAGCA TTTTGGCCCA GCTGCAGCAG CGCCAGCACC TGCTCAAGCT GAGAAAGGAG 
CAGGCCAATC ACCCGGGAGC AGCCACCACG GATGCCACCT GGCTGAAGGA GCTGCAGGCG 
AAACAGGCAA ACGATTTGAG AGCCATGCAA CGTAAGCTGG AGGCCTCGTC GCCATCAAGT 
GTCAGGGACT TGACTCATCG TTTCGAGCAA ACCTCCATTC GATCCTACGC CTCGCAGGAG 
CTATTGTCCC AGCCGGGACA GAGTCAGCAG CTGGGGCAGC TGCGCACCCA GATGAATGGG 
CTGCCAAACG GCAATGCAAA AATGGATGTA GATGAGGTGG ACGCTATGCC AGCGGTGAGA 
AACTCTCTGC CCGCCGCACT CAGCGCCCAC CAATTGCTGC CCAAGCCTAA GTACGAAATG 
ACCCAATCGC AGATTGCAGA GGAGATTCGC GAAGTGGAGT TGCTGAACAC GATGGTACAG 
CAGACACTGA ATCAGAACGG AGCTGCCGCA CTACCTAAGC GGGTAAAGAA AAAGAGCGTC 
TCCTTTTGCG ACCAGGTGAT CCTGGTTGCC ACAGCCGGAA ACGAGGAGGA CGATGACTTT 
ATACCCAATC CCATACTGGA GCGGGTCCTG CGGACTGCCC AGCATCCCAA CGAAAAAGTC 
ACTGCTCAAA TGATCCAGCA GCAGCAGCAG AACATGATGC GATTGCAAAC AGAAGCGCAG 
CCGCGGCAAC AGCAGGTGCA GCAGCTGCAA CAACCATCAC CCATGTTGGG CAGACCAGCA 
GGAGCCACAG CAGATATGCA GCGATACGTC CAGAGGATGC AGCAACAACA GCAGCAGCAG 
CAACAACAGC AGCAGCAACA ACAGCAGCAG CAGCAACAAC AGCAGCAACA ACAGCAGCAG 
CAACAGCAGC AGCAGCAGCA ACACCAGGAG TTGTACCGCC AGCAGCAGCT ACATCGCACT 
AGTATGAGCG GCATAGCCAG CCAGCAACAC TTGCAGCAAT TGGATGCACA GTACACCACC 
ATGCCGCGTC CCTTGCATCA TTCACAGCTT GGTAGCTACT CTGTGACCTT GCAGAAACAC 
CAGCAACTTC AGCAGCAACA GCAACAGCAG CAACAGCAGC AGGCCCCGCA GCAGCTCAGC 
TTGGGCTACT TTAGCAATGG CAGTGTTGCG ACTTCAGAGC AAGTCCCGCT GCCTTCGCCC 
TACCAGCGGG TGCCCCTGCC ACATGGATAC CAGCCTGCAG CAGCAGGACC CTACTACCCG 
CTACCCAATG CCCAGCAACA TCTGCAGCAG CAACAGCTGA TGGCTAAGCC CGTGCAGAAA 
AAGGTGAGCT TTGAGCCAGG AACTAAAGGC GAAGCCGATT GCTTGCCCCC ACCACCGCCG 
CCGCCGCAAA AGAACGGCTT GCCCGAGACG GGATCTGCAG GATCAGGACA CCCTGGAGCA 
GCACCCGGGG CAAGTGGAGC GGGCGGCACC ACATCGGCCG GCATCACCGC CATTCCCACC 
CGCGTCTACA ACAATGCCAT TGTAAAGGCT TCGGCCAAAG CCGTTGAGTG CAATTTGTGC 
CGCAAGCGGC ATGTGATCGC ACCGGCGGTA TATTGCACCA ACTGCGAGTA CTACCTGCAG 
ATGCTGAACC AGCGCAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ec ( NM_166992.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726893.2 Drosophila melanogaster echinus (ec), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa00728
Clone ID Related Accession (Same CDS sequence)	NM_166992.3
Accession Version	NM_166992.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	echinus, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_166992.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATACGG CGGCCATTAA AACATCGCTG CCACATGCCC CACTGGCGGG GCAGCAACAA 
CCGCAGCAGG CGCAGACAAC AACAGCTATG GCAGTAGCGC CAACAACAAT AACAAATGCC 
CAAACATCGG CAATCAATGC CAAAAACAAC ATTGGCGCAT TCTTTCAGAC CTGCAAGGTC 
TTGTGGCACT TGGACGCCTT TCGACGATCG TTTCGCGGCC TAAATCAGCA CGTATGCGGT 
GGCCAAGACT GTATCTTTTG CGCTCTAAAG GAACTTTTCC AGCAGCTGCA GACCAGTTCT 
GAGCCCGCCT TGTGCCCGGA GCCACTGCGT CGGGCTTTGG CCAGTGGTCC ATTGGCTGGA 
CGGCGATTCC CACTCGGCTG CTTGGGCGAT GCAGCCGAAT GCTTCGAGCT CCTTCTCCAC 
CGCGTCCACT CGCACATTTC GCCGGATGAC GGCGACTCCT GCGAATCCTC CGCCTGCATT 
GCCCACCGAA GATTCGCCAT GCGCGTCATC GAGCAGAGCG TCTGCAAGTG CGGAGCCAAT 
TCGGAGCAGC TGCCCTTCAC CCAGATGGTG CACTACGTCT CTGCCTCGGC TCTGACCTCC 
CAGAAAAGTC TGGCACTGCA GTCCCATCAG CAACTGAGTT TTGGCCAGCT TTTAAGGGCC 
GCCGGCAACA TGGGCGACAT ACGCGATTGT CCGAACACCT GCGGCGCCAA GATTGGAATT 
TGTCGTGCGC TGCTTAACCG ACCAGAAGTG GTGTCCATTG GCATCGTTTG GGACTCTGAG 
CGACCGGCGG CGGATCAGGT TCATGCCGTC CTAAAGGCAG TGGGCACCAG CCTGCGTCTG 
GGTGACGTTT TTCACCAGGT CAGCGAGCCA CGATGGGCCC AGCAGACCCA GCACGAGCTG 
GTTGGCATCG TTTCCTACTA CGGCAAACAC TACACCACCT TCTTCTTCCA CACGAAGCTC 
AAGGTGTGGG TCTACTTCGA TGATGCGAAC GTTAAGGAGG TGGGGCCCAG TTGGGAGGGC 
GTGGTGGACA AATGCAGCCG GGGAAGATAT CAGCCACTCC TGCTGCTGTA CGCCGTTCCC 
CAGCAGCCTA CGGTGGGCAA CGGCGTAGGT ATCGGTCACG TGGATGTGGC CATGCCGGCG 
AGTGCTCCAT CGATAGCTGG CTCAGTGGTT CGCAGGGCAG TTACGCCCAG TCCGGAGAAG 
CCCAGCTTGG GCAACACAAG GAGGGCCATC ACCCCCACTC CGCTGAGGAC ACCACCCAAC 
GACTATCAGA ACCTGAGTGT GATTCAAAAG AATATCTTTC CGAGCAATCT GGCAATGGGT 
ACGGATGAAA CGGATGCCTA CATTAGCCGC AAGACGGTTG AACATGTGCT GAGTGCCCAG 
TACCAGAATT TGAGCGTAAT CCAGGACAAG ATTGGAGGCG GCAATGGAGT CGGTGTCGGC 
CAACAGAGTG TGGATAAGGA TGGAGGCTTT CTGAGCCGAA AGCCCATTGA GGCAGTGCTG 
AGTGCCCAAC TGGCGCGGAG GCAGCACCTC CAGCTTCAAC GCAGCCACAG TGCGGAGTCA 
AGCTCCGGCC ACGCCTCCTC CAACGGCAGT TCTCCGCCCA GCGATGGACT GACCATGCCG 
GAGCACCTGA ATCAGCCACG TCGTCGCGAC TCGGGCAATT GGTCAGGCGA TCGAAACAGC 
GCTAGTTCGG CCAGTTCCAC GACGCTGGAT AATCCCTACC TGTACATGGT GGCCAAGCGG 
GGTGTGGCAG CAGTGCCACA GAGCCCCACG CGACATGGTT TGCCATATGA CCCGGGCTAC 
GATTCGTTTT CGCTAAGCTC CACGGACTCA TATCCGCCGA AGCATGCGCT AAATCCACAG 
CTGGCTAAAA TCCCGGAAGC AGCCATGGGC GTCGTTCCAA ACGGAGGAGC AATGCCCCAT 
GGACATGGCA ACGGACAGCT GGCCTTGTCT GGGGACTGCG AGAAGCTGTG CCACGAGGCG 
GACCAGTTGC TGGAAAAGTC CCGACTGGTG GAGGAGTCAC ATGATCTGGA AACAGCCCTG 
GTGCTCTGCA ATGCGGCTGC TGGGCGGGCA CGAGCCGCTA TGGATGCCCC GTATAGCAAT 
CCGCACACGA TGACCTTTGC CCGGATGAAG CACAATACTT GCGTGATGCG GGCCCGTAGT 
CTGCACCGGC GGATTCTGGT GGAGAAGGGA GCTGAAACAG AGGCTATGCC CGAACTGAAG 
CACATGCGCG AAGGAAGCAC GAGTAGTGTG AAGCATGTGC GCCAGAATAG CAAGGATCGC 
ACGTTAGAGA AGGAGCAGCA GCAGCTGCAG TTCCAGCAAA TACAGCAGAT CCAGCAGATT 
CATCAGATCC AGCAGCAGAT CCCAGCCAGC ATCGAAATAT ATGCCACGCT CCCGAAGCGC 
AAGAGTCCTC TGAAGGCCTT GGCCTCTGCG TCTGCGTGCG ATGACAACGC AATCGAGTAC 
GAGCAGGAGA AGCAACTGGC CACCAGTCCG GGAAAACCGG AGCGCGAGAG TCGCTCGCTG 
TTCGGGCGCA AGGACAAAGA GAAGGAGAAG CGGAGTCGCA GCGAGGATCG CAACAAGCTA 
ACCCGTGAGT TCTCGCTCAC CGAAACGCTT CTAGTGAATG CCAAGGATAC GCTGAAGAAG 
CATAAGGAGG ACAAGGACGA GAAGAAGGAG AAGGACAAGA GCGGCAAAAA GCAGCACAAA 
ATCAGACGCA AGCTGTTAAT GGGCGGGCTC ATTCGTCGCA AGAATCGCTC CATGCCGGAT 
CTCACAGAGG CGGTGGATGA CTCGACGGCC AATGCCGGCA TAACGCACCA TCCACATTAT 
CCTGCACAGG GCGTAACTAC GGCGGGCACT CTCTTGGGTA CCTCCGTAGA CGACAGTGCC 
GTTGGATTGG GCAAACACGG CCACGGCATG AGCGGCTACA TCTCTGAGGG ACACTTCGAC 
TACTCGGGTT CCGGGTCGGC CTCAAATTCG GGAGCCGGCA GCAATCCAAA TCCGAATCTG 
GAGAGGAGCA AGCTGATGCG CAAGAGCTTC CACGGCAGCG GCAGGCAGTT GACAATGGTA 
CCGAAAGTGG CTCCACCGCC GCCCATGCGA ACCAGCTCCA CTTTGACCCC ACAGCAGCAA 
CAACAACAAC AACAGCAATT TCACCATGAG GCCAACTTGT CCAACCTCTC GGCCATGAGT 
TCAAACACCT CGATTAGCGA GGATTCCTGC CAGACGATCA TCACCACTTG CGCACAGGTG 
CACTCGGAGC AGAGTCCACT AAAGGATCTG CAAATGCTGG TCGCTCAGGA CGAGCTGCCA 
CTGCCGCCGC CAATGGAACT TCCTCCGTAC CCAAGTCCAC CGCACTCCTC CTGCCATTCG 
CGGCAGGCTA GCGAAGATTT CCCGCCGCCA CCACCGCCGG AACTGGATCT GGAGCCACTC 
AACCAGCAAC TGAGCCAGCT GCAGGCACTG GAGGCGGCCA GTAAGCAGCA GCGCCAGCAA 
CTCGAGTCCC TCGAGGGCAC CACCAGCATT TTGGCCCAGC TGCAGCAGCG CCAGCACCTG 
CTCAAGCTGA GAAAGGAGCA GGCCAATCAC CCGGGAGCAG CCACCACGGA TGCCACCTGG 
CTGAAGGAGC TGCAGGCGAA ACAGGCAAAC GATTTGAGAG CCATGCAACG TAAGCTGGAG 
GCCTCGTCGC CATCAAGTGT CAGGGACTTG ACTCATCGTT TCGAGCAAAC CTCCATTCGA 
TCCTACGCCT CGCAGGAGCT ATTGTCCCAG CCGGGACAGA GTCAGCAGCT GGGGCAGCTG 
CGCACCCAGA TGAATGGGCT GCCAAACGGC AATGCAAAAA TGGATGTAGA TGAGGTGGAC 
GCTATGCCAG CGGTGAGAAA CTCTCTGCCC GCCGCACTCA GCGCCCACCA ATTGCTGCCC 
AAGCCTAAGT ACGAAATGAC CCAATCGCAG ATTGCAGAGG AGATTCGCGA AGTGGAGTTG 
CTGAACACGA TGGTACAGCA GACACTGAAT CAGAACGGAG CTGCCGCACT ACCTAAGCGG 
GTAAAGAAAA AGAGCGTCTC CTTTTGCGAC CAGGTGATCC TGGTTGCCAC AGCCGGAAAC 
GAGGAGGACG ATGACTTTAT ACCCAATCCC ATACTGGAGC GGGTCCTGCG GACTGCCCAG 
CATCCCAACG AAAAAGTCAC TGCTCAAATG ATCCAGCAGC AGCAGCAGAA CATGATGCGA 
TTGCAAACAG AAGCGCAGCC GCGGCAACAG CAGGTGCAGC AGCTGCAACA ACCATCACCC 
ATGTTGGGCA GACCAGCAGG AGCCACAGCA GATATGCAGC GATACGTCCA GAGGATGCAG 
CAACAACAGC AGCAGCAGCA ACAACAGCAG CAGCAACAAC AGCAGCAGCA GCAACAACAG 
CAGCAACAAC AGCAGCAGCA ACAGCAGCAG CAGCAGCAAC ACCAGGAGTT GTACCGCCAG 
CAGCAGCTAC ATCGCACTAG TATGAGCGGC ATAGCCAGCC AGCAACACTT GCAGCAATTG 
GATGCACAGT ACACCACCAT GCCGCGTCCC TTGCATCATT CACAGCTTGG TAGCTACTCT 
GTGACCTTGC AGAAACACCA GCAACTTCAG CAGCAACAGC AACAGCAGCA ACAGCAGCAG 
GCCCCGCAGC AGCTCAGCTT GGGCTACTTT AGCAATGGCA GTGTTGCGAC TTCAGAGCAA 
GTCCCGCTGC CTTCGCCCTA CCAGCGGGTG CCCCTGCCAC ATGGATACCA GCCTGCAGCA 
GCAGGACCCT ACTACCCGCT ACCCAATGCC CAGCAACATC TGCAGCAGCA ACAGCTGATG 
GCTAAGCCCG TGCAGAAAAA GGTGAGCTTT GAGCCAGGAA CTAAAGGCGA AGCCGATTGC 
TTGCCCCCAC CACCGCCGCC GCCGCAAAAG AACGGCTTGC CCGAGACGGG ATCTGCAGGA 
TCAGGACACC CTGGAGCAGC ACCCGGGGCA AGTGGAGCGG GCGGCACCAC ATCGGCCGGC 
ATCACCGCCA TTCCCACCCG CGTCTACAAC AATGCCATTG TAAAGGCTTC GGCCAAAGCC 
GTTGAGTGCA ATTTGTGCCG CAAGCGGCAT GTGATCGCAC CGGCGGTATA TTGCACCAAC 
TGCGAGTACT ACCTGCAGAT GCTGAACCAG CGCAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726893.2
CDS	585..5723
Translation	MDTAAIKTSLPHAPLAGQQQPQQAQTTTAMAVAPTTITNAQTSAINAKNNIGAFFQTCKVLWHLDAFRRSFRGLNQHVCGGQDCIFCALKELFQQLQTSSEPALCPEPLRRALASGPLAGRRFPLGCLGDAAECFELLLHRVHSHISPDDGDSCESSACIAHRRFAMRVIEQSVCKCGANSEQLPFTQMVHYVSASALTSQKSLALQSHQQLSFGQLLRAAGNMGDIRDCPNTCGAKIGICRALLNRPEVVSIGIVWDSERPAADQVHAVLKAVGTSLRLGDVFHQVSEPRWAQQTQHELVGIVSYYGKHYTTFFFHTKLKVWVYFDDANVKEVGPSWEGVVDKCSRGRYQPLLLLYAVPQQPTVGNGVGIGHVDVAMPASAPSIAGSVVRRAVTPSPEKPSLGNTRRAITPTPLRTPPNDYQNLSVIQKNIFPSNLAMGTDETDAYISRKTVEHVLSAQYQNLSVIQDKIGGGNGVGVGQQSVDKDGGFLSRKPIEAVLSAQLARRQHLQLQRSHSAESSSGHASSNGSSPPSDGLTMPEHLNQPRRRDSGNWSGDRNSASSASSTTLDNPYLYMVAKRGVAAVPQSPTRHGLPYDPGYDSFSLSSTDSYPPKHALNPQLAKIPEAAMGVVPNGGAMPHGHGNGQLALSGDCEKLCHEADQLLEKSRLVEESHDLETALVLCNAAAGRARAAMDAPYSNPHTMTFARMKHNTCVMRARSLHRRILVEKGAETEAMPELKHMREGSTSSVKHVRQNSKDRTLEKEQQQLQFQQIQQIQQIHQIQQQIPASIEIYATLPKRKSPLKALASASACDDNAIEYEQEKQLATSPGKPERESRSLFGRKDKEKEKRSRSEDRNKLTREFSLTETLLVNAKDTLKKHKEDKDEKKEKDKSGKKQHKIRRKLLMGGLIRRKNRSMPDLTEAVDDSTANAGITHHPHYPAQGVTTAGTLLGTSVDDSAVGLGKHGHGMSGYISEGHFDYSGSGSASNSGAGSNPNPNLERSKLMRKSFHGSGRQLTMVPKVAPPPPMRTSSTLTPQQQQQQQQQFHHEANLSNLSAMSSNTSISEDSCQTIITTCAQVHSEQSPLKDLQMLVAQDELPLPPPMELPPYPSPPHSSCHSRQASEDFPPPPPPELDLEPLNQQLSQLQALEAASKQQRQQLESLEGTTSILAQLQQRQHLLKLRKEQANHPGAATTDATWLKELQAKQANDLRAMQRKLEASSPSSVRDLTHRFEQTSIRSYASQELLSQPGQSQQLGQLRTQMNGLPNGNAKMDVDEVDAMPAVRNSLPAALSAHQLLPKPKYEMTQSQIAEEIREVELLNTMVQQTLNQNGAAALPKRVKKKSVSFCDQVILVATAGNEEDDDFIPNPILERVLRTAQHPNEKVTAQMIQQQQQNMMRLQTEAQPRQQQVQQLQQPSPMLGRPAGATADMQRYVQRMQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQHQELYRQQQLHRTSMSGIASQQHLQQLDAQYTTMPRPLHHSQLGSYSVTLQKHQQLQQQQQQQQQQQAPQQLSLGYFSNGSVATSEQVPLPSPYQRVPLPHGYQPAAAGPYYPLPNAQQHLQQQQLMAKPVQKKVSFEPGTKGEADCLPPPPPPPQKNGLPETGSAGSGHPGAAPGASGAGGTTSAGITAIPTRVYNNAIVKASAKAVECNLCRKRHVIAPAVYCTNCEYYLQMLNQRR

Target ORF information:

RefSeq Version	NM_166992.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster echinus (ec), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166992.3

ORF Insert Sequence:

ATGGATACGG CGGCCATTAA AACATCGCTG CCACATGCCC CACTGGCGGG GCAGCAACAA 
CCGCAGCAGG CGCAGACAAC AACAGCTATG GCAGTAGCGC CAACAACAAT AACAAATGCC 
CAAACATCGG CAATCAATGC CAAAAACAAC ATTGGCGCAT TCTTTCAGAC CTGCAAGGTC 
TTGTGGCACT TGGACGCCTT TCGACGATCG TTTCGCGGCC TAAATCAGCA CGTATGCGGT 
GGCCAAGACT GTATCTTTTG CGCTCTAAAG GAACTTTTCC AGCAGCTGCA GACCAGTTCT 
GAGCCCGCCT TGTGCCCGGA GCCACTGCGT CGGGCTTTGG CCAGTGGTCC ATTGGCTGGA 
CGGCGATTCC CACTCGGCTG CTTGGGCGAT GCAGCCGAAT GCTTCGAGCT CCTTCTCCAC 
CGCGTCCACT CGCACATTTC GCCGGATGAC GGCGACTCCT GCGAATCCTC CGCCTGCATT 
GCCCACCGAA GATTCGCCAT GCGCGTCATC GAGCAGAGCG TCTGCAAGTG CGGAGCCAAT 
TCGGAGCAGC TGCCCTTCAC CCAGATGGTG CACTACGTCT CTGCCTCGGC TCTGACCTCC 
CAGAAAAGTC TGGCACTGCA GTCCCATCAG CAACTGAGTT TTGGCCAGCT TTTAAGGGCC 
GCCGGCAACA TGGGCGACAT ACGCGATTGT CCGAACACCT GCGGCGCCAA GATTGGAATT 
TGTCGTGCGC TGCTTAACCG ACCAGAAGTG GTGTCCATTG GCATCGTTTG GGACTCTGAG 
CGACCGGCGG CGGATCAGGT TCATGCCGTC CTAAAGGCAG TGGGCACCAG CCTGCGTCTG 
GGTGACGTTT TTCACCAGGT CAGCGAGCCA CGATGGGCCC AGCAGACCCA GCACGAGCTG 
GTTGGCATCG TTTCCTACTA CGGCAAACAC TACACCACCT TCTTCTTCCA CACGAAGCTC 
AAGGTGTGGG TCTACTTCGA TGATGCGAAC GTTAAGGAGG TGGGGCCCAG TTGGGAGGGC 
GTGGTGGACA AATGCAGCCG GGGAAGATAT CAGCCACTCC TGCTGCTGTA CGCCGTTCCC 
CAGCAGCCTA CGGTGGGCAA CGGCGTAGGT ATCGGTCACG TGGATGTGGC CATGCCGGCG 
AGTGCTCCAT CGATAGCTGG CTCAGTGGTT CGCAGGGCAG TTACGCCCAG TCCGGAGAAG 
CCCAGCTTGG GCAACACAAG GAGGGCCATC ACCCCCACTC CGCTGAGGAC ACCACCCAAC 
GACTATCAGA ACCTGAGTGT GATTCAAAAG AATATCTTTC CGAGCAATCT GGCAATGGGT 
ACGGATGAAA CGGATGCCTA CATTAGCCGC AAGACGGTTG AACATGTGCT GAGTGCCCAG 
TACCAGAATT TGAGCGTAAT CCAGGACAAG ATTGGAGGCG GCAATGGAGT CGGTGTCGGC 
CAACAGAGTG TGGATAAGGA TGGAGGCTTT CTGAGCCGAA AGCCCATTGA GGCAGTGCTG 
AGTGCCCAAC TGGCGCGGAG GCAGCACCTC CAGCTTCAAC GCAGCCACAG TGCGGAGTCA 
AGCTCCGGCC ACGCCTCCTC CAACGGCAGT TCTCCGCCCA GCGATGGACT GACCATGCCG 
GAGCACCTGA ATCAGCCACG TCGTCGCGAC TCGGGCAATT GGTCAGGCGA TCGAAACAGC 
GCTAGTTCGG CCAGTTCCAC GACGCTGGAT AATCCCTACC TGTACATGGT GGCCAAGCGG 
GGTGTGGCAG CAGTGCCACA GAGCCCCACG CGACATGGTT TGCCATATGA CCCGGGCTAC 
GATTCGTTTT CGCTAAGCTC CACGGACTCA TATCCGCCGA AGCATGCGCT AAATCCACAG 
CTGGCTAAAA TCCCGGAAGC AGCCATGGGC GTCGTTCCAA ACGGAGGAGC AATGCCCCAT 
GGACATGGCA ACGGACAGCT GGCCTTGTCT GGGGACTGCG AGAAGCTGTG CCACGAGGCG 
GACCAGTTGC TGGAAAAGTC CCGACTGGTG GAGGAGTCAC ATGATCTGGA AACAGCCCTG 
GTGCTCTGCA ATGCGGCTGC TGGGCGGGCA CGAGCCGCTA TGGATGCCCC GTATAGCAAT 
CCGCACACGA TGACCTTTGC CCGGATGAAG CACAATACTT GCGTGATGCG GGCCCGTAGT 
CTGCACCGGC GGATTCTGGT GGAGAAGGGA GCTGAAACAG AGGCTATGCC CGAACTGAAG 
CACATGCGCG AAGGAAGCAC GAGTAGTGTG AAGCATGTGC GCCAGAATAG CAAGGATCGC 
ACGTTAGAGA AGGAGCAGCA GCAGCTGCAG TTCCAGCAAA TACAGCAGAT CCAGCAGATT 
CATCAGATCC AGCAGCAGAT CCCAGCCAGC ATCGAAATAT ATGCCACGCT CCCGAAGCGC 
AAGAGTCCTC TGAAGGCCTT GGCCTCTGCG TCTGCGTGCG ATGACAACGC AATCGAGTAC 
GAGCAGGAGA AGCAACTGGC CACCAGTCCG GGAAAACCGG AGCGCGAGAG TCGCTCGCTG 
TTCGGGCGCA AGGACAAAGA GAAGGAGAAG CGGAGTCGCA GCGAGGATCG CAACAAGCTA 
ACCCGTGAGT TCTCGCTCAC CGAAACGCTT CTAGTGAATG CCAAGGATAC GCTGAAGAAG 
CATAAGGAGG ACAAGGACGA GAAGAAGGAG AAGGACAAGA GCGGCAAAAA GCAGCACAAA 
ATCAGACGCA AGCTGTTAAT GGGCGGGCTC ATTCGTCGCA AGAATCGCTC CATGCCGGAT 
CTCACAGAGG CGGTGGATGA CTCGACGGCC AATGCCGGCA TAACGCACCA TCCACATTAT 
CCTGCACAGG GCGTAACTAC GGCGGGCACT CTCTTGGGTA CCTCCGTAGA CGACAGTGCC 
GTTGGATTGG GCAAACACGG CCACGGCATG AGCGGCTACA TCTCTGAGGG ACACTTCGAC 
TACTCGGGTT CCGGGTCGGC CTCAAATTCG GGAGCCGGCA GCAATCCAAA TCCGAATCTG 
GAGAGGAGCA AGCTGATGCG CAAGAGCTTC CACGGCAGCG GCAGGCAGTT GACAATGGTA 
CCGAAAGTGG CTCCACCGCC GCCCATGCGA ACCAGCTCCA CTTTGACCCC ACAGCAGCAA 
CAACAACAAC AACAGCAATT TCACCATGAG GCCAACTTGT CCAACCTCTC GGCCATGAGT 
TCAAACACCT CGATTAGCGA GGATTCCTGC CAGACGATCA TCACCACTTG CGCACAGGTG 
CACTCGGAGC AGAGTCCACT AAAGGATCTG CAAATGCTGG TCGCTCAGGA CGAGCTGCCA 
CTGCCGCCGC CAATGGAACT TCCTCCGTAC CCAAGTCCAC CGCACTCCTC CTGCCATTCG 
CGGCAGGCTA GCGAAGATTT CCCGCCGCCA CCACCGCCGG AACTGGATCT GGAGCCACTC 
AACCAGCAAC TGAGCCAGCT GCAGGCACTG GAGGCGGCCA GTAAGCAGCA GCGCCAGCAA 
CTCGAGTCCC TCGAGGGCAC CACCAGCATT TTGGCCCAGC TGCAGCAGCG CCAGCACCTG 
CTCAAGCTGA GAAAGGAGCA GGCCAATCAC CCGGGAGCAG CCACCACGGA TGCCACCTGG 
CTGAAGGAGC TGCAGGCGAA ACAGGCAAAC GATTTGAGAG CCATGCAACG TAAGCTGGAG 
GCCTCGTCGC CATCAAGTGT CAGGGACTTG ACTCATCGTT TCGAGCAAAC CTCCATTCGA 
TCCTACGCCT CGCAGGAGCT ATTGTCCCAG CCGGGACAGA GTCAGCAGCT GGGGCAGCTG 
CGCACCCAGA TGAATGGGCT GCCAAACGGC AATGCAAAAA TGGATGTAGA TGAGGTGGAC 
GCTATGCCAG CGGTGAGAAA CTCTCTGCCC GCCGCACTCA GCGCCCACCA ATTGCTGCCC 
AAGCCTAAGT ACGAAATGAC CCAATCGCAG ATTGCAGAGG AGATTCGCGA AGTGGAGTTG 
CTGAACACGA TGGTACAGCA GACACTGAAT CAGAACGGAG CTGCCGCACT ACCTAAGCGG 
GTAAAGAAAA AGAGCGTCTC CTTTTGCGAC CAGGTGATCC TGGTTGCCAC AGCCGGAAAC 
GAGGAGGACG ATGACTTTAT ACCCAATCCC ATACTGGAGC GGGTCCTGCG GACTGCCCAG 
CATCCCAACG AAAAAGTCAC TGCTCAAATG ATCCAGCAGC AGCAGCAGAA CATGATGCGA 
TTGCAAACAG AAGCGCAGCC GCGGCAACAG CAGGTGCAGC AGCTGCAACA ACCATCACCC 
ATGTTGGGCA GACCAGCAGG AGCCACAGCA GATATGCAGC GATACGTCCA GAGGATGCAG 
CAACAACAGC AGCAGCAGCA ACAACAGCAG CAGCAACAAC AGCAGCAGCA GCAACAACAG 
CAGCAACAAC AGCAGCAGCA ACAGCAGCAG CAGCAGCAAC ACCAGGAGTT GTACCGCCAG 
CAGCAGCTAC ATCGCACTAG TATGAGCGGC ATAGCCAGCC AGCAACACTT GCAGCAATTG 
GATGCACAGT ACACCACCAT GCCGCGTCCC TTGCATCATT CACAGCTTGG TAGCTACTCT 
GTGACCTTGC AGAAACACCA GCAACTTCAG CAGCAACAGC AACAGCAGCA ACAGCAGCAG 
GCCCCGCAGC AGCTCAGCTT GGGCTACTTT AGCAATGGCA GTGTTGCGAC TTCAGAGCAA 
GTCCCGCTGC CTTCGCCCTA CCAGCGGGTG CCCCTGCCAC ATGGATACCA GCCTGCAGCA 
GCAGGACCCT ACTACCCGCT ACCCAATGCC CAGCAACATC TGCAGCAGCA ACAGCTGATG 
GCTAAGCCCG TGCAGAAAAA GGTGAGCTTT GAGCCAGGAA CTAAAGGCGA AGCCGATTGC 
TTGCCCCCAC CACCGCCGCC GCCGCAAAAG AACGGCTTGC CCGAGACGGG ATCTGCAGGA 
TCAGGACACC CTGGAGCAGC ACCCGGGGCA AGTGGAGCGG GCGGCACCAC ATCGGCCGGC 
ATCACCGCCA TTCCCACCCG CGTCTACAAC AATGCCATTG TAAAGGCTTC GGCCAAAGCC 
GTTGAGTGCA ATTTGTGCCG CAAGCGGCAT GTGATCGCAC CGGCGGTATA TTGCACCAAC 
TGCGAGTACT ACCTGCAGAT GCTGAACCAG CGCAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ec ( NM_001103407.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001096877.1 Drosophila melanogaster echinus (ec), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa00729
Clone ID Related Accession (Same CDS sequence)	NM_001103407.3
Accession Version	NM_001103407.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	echinus, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001103407.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATCAAGT TCCGCTACAA AAGGAAAGAA CCCACGAATG TGGTGGGCGT AGCAGCCCCA 
ACTGCAGGAG CAGCAGCATT GGGATCAGGA GCATCCAGCA CCCCGGGCAC ATCCCCAACA 
TCACCTGTTT CCGTTCCCGT TCCCGTTCAA GCCCACCATC CCCATCCCCA GTTGGGGCAC 
CTGATCAGCA ACAATGGAGG CACCCTGCCA AGGAGTAGTC CCCGCAAATT AATCCTGGAT 
GGAGCCGACA AGTCGGCCAC ATTGACCCGC CAGCAGTCGA AGCAGAAGTC GGTCAGTGCC 
TTGGCCAAAG TGGCCAGCAG CGTGGAATTG GCCGTAATGG GCTATAATAG CACTGGCAAT 
GGCAATAGCA ATGGTCATAT CAACAGCGCG GGAAATTCGG AGAGGGATCG GTGCATCAAT 
GCGGTTATCG AACTTTTCCA GCAGCTGCAG ACCAGTTCTG AGCCCGCCTT GTGCCCGGAG 
CCACTGCGTC GGGCTTTGGC CAGTGGTCCA TTGGCTGGAC GGCGATTCCC ACTCGGCTGC 
TTGGGCGATG CAGCCGAATG CTTCGAGCTC CTTCTCCACC GCGTCCACTC GCACATTTCG 
CCGGATGACG GCGACTCCTG CGAATCCTCC GCCTGCATTG CCCACCGAAG ATTCGCCATG 
CGCGTCATCG AGCAGAGCGT CTGCAAGTGC GGAGCCAATT CGGAGCAGCT GCCCTTCACC 
CAGATGGTGC ACTACGTCTC TGCCTCGGCT CTGACCTCCC AGAAAAGTCT GGCACTGCAG 
TCCCATCAGC AACTGAGTTT TGGCCAGCTT TTAAGGGCCG CCGGCAACAT GGGCGACATA 
CGCGATTGTC CGAACACCTG CGGCGCCAAG ATTGGAATTT GTCGTGCGCT GCTTAACCGA 
CCAGAAGTGG TGTCCATTGG CATCGTTTGG GACTCTGAGC GACCGGCGGC GGATCAGGTT 
CATGCCGTCC TAAAGGCAGT GGGCACCAGC CTGCGTCTGG GTGACGTTTT TCACCAGGTC 
AGCGAGCCAC GATGGGCCCA GCAGACCCAG CACGAGCTGG TTGGCATCGT TTCCTACTAC 
GGCAAACACT ACACCACCTT CTTCTTCCAC ACGAAGCTCA AGGTGTGGGT CTACTTCGAT 
GATGCGAACG TTAAGGAGGT GGGGCCCAGT TGGGAGGGCG TGGTGGACAA ATGCAGCCGG 
GGAAGATATC AGCCACTCCT GCTGCTGTAC GCCGTTCCCC AGCAGCCTAC GGTGGGCAAC 
GGCGTAGGTA TCGGTCACGT GGATGTGGCC ATGCCGGCGA GTGCTCCATC GATAGCTGGC 
TCAGTGGTTC GCAGGGCAGT TACGCCCAGT CCGGAGAAGC CCAGCTTGGG CAACACAAGG 
AGGGCCATCA CCCCCACTCC GCTGAGGACA CCACCCAACG ACTATCAGAA CCTGAGTGTG 
ATTCAAAAGA ATATCTTTCC GAGCAATCTG GCAATGGGTA CGGATGAAAC GGATGCCTAC 
ATTAGCCGCA AGACGGTTGA ACATGTGCTG AGTGCCCAGT ACCAGAATTT GAGCGTAATC 
CAGGACAAGA TTGGAGGCGG CAATGGAGTC GGTGTCGGCC AACAGAGTGT GGATAAGGAT 
GGAGGCTTTC TGAGCCGAAA GCCCATTGAG GCAGTGCTGA GTGCCCAACT GGCGCGGAGG 
CAGCACCTCC AGCTTCAACG CAGCCACAGT GCGGAGTCAA GCTCCGGCCA CGCCTCCTCC 
AACGGCAGTT CTCCGCCCAG CGATGGACTG ACCATGCCGG AGCACCTGAA TCAGCCACGT 
CGTCGCGACT CGGGCAATTG GTCAGGCGAT CGAAACAGCG CTAGTTCGGC CAGTTCCACG 
ACGCTGGATA ATCCCTACCT GTACATGGTG GCCAAGCGGG GTGTGGCAGC AGTGCCACAG 
AGCCCCACGC GACATGGTTT GCCATATGAC CCGGGCTACG ATTCGTTTTC GCTAAGCTCC 
ACGGACTCAT ATCCGCCGAA GCATGCGCTA AATCCACAGC TGGCTAAAAT CCCGGAAGCA 
GCCATGGGCG TCGTTCCAAA CGGAGGAGCA ATGCCCCATG GACATGGCAA CGGACAGCTG 
GCCTTGTCTG GGGACTGCGA GAAGCTGTGC CACGAGGCGG ACCAGTTGCT GGAAAAGTCC 
CGACTGGTGG AGGAGTCACA TGATCTGGAA ACAGCCCTGG TGCTCTGCAA TGCGGCTGCT 
GGGCGGGCAC GAGCCGCTAT GGATGCCCCG TATAGCAATC CGCACACGAT GACCTTTGCC 
CGGATGAAGC ACAATACTTG CGTGATGCGG GCCCGTAGTC TGCACCGGCG GATTCTGGTG 
GAGAAGGGAG CTGAAACAGA GGCTATGCCC GAACTGAAGC ACATGCGCGA AGGAAGCACG 
AGTAGTGTGA AGCATGTGCG CCAGAATAGC AAGGATCGCA CGTTAGAGAA GGAGCAGCAG 
CAGCTGCAGT TCCAGCAAAT ACAGCAGATC CAGCAGATTC ATCAGATCCA GCAGCAGATC 
CCAGCCAGCA TCGAAATATA TGCCACGCTC CCGAAGCGCA AGAGTCCTCT GAAGGCCTTG 
GCCTCTGCGT CTGCGTGCGA TGACAACGCA ATCGAGTACG AGCAGGAGAA GCAACTGGCC 
ACCAGTCCGG GAAAACCGGA GCGCGAGAGT CGCTCGCTGT TCGGGCGCAA GGACAAAGAG 
AAGGAGAAGC GGAGTCGCAG CGAGGATCGC AACAAGCTAA CCCGTGAGTT CTCGCTCACC 
GAAACGCTTC TAGTGAATGC CAAGGATACG CTGAAGAAGC ATAAGGAGGA CAAGGACGAG 
AAGAAGGAGA AGGACAAGAG CGGCAAAAAG CAGCACAAAA TCAGACGCAA GCTGTTAATG 
GGCGGGCTCA TTCGTCGCAA GAATCGCTCC ATGCCGGATC TCACAGAGGC GGTGGATGAC 
TCGACGGCCA ATGCCGGCAT AACGCACCAT CCACATTATC CTGCACAGGG CGTAACTACG 
GCGGGCACTC TCTTGGGTAC CTCCGTAGAC GACAGTGCCG TTGGATTGGG CAAACACGGC 
CACGGCATGA GCGGCTACAT CTCTGAGGGA CACTTCGACT ACTCGGGTTC CGGGTCGGCC 
TCAAATTCGG GAGCCGGCAG CAATCCAAAT CCGAATCTGG AGAGGAGCAA GCTGATGCGC 
AAGAGCTTCC ACGGCAGCGG CAGGCAGTTG ACAATGGTAC CGAAAGTGGC TCCACCGCCG 
CCCATGCGAA CCAGCTCCAC TTTGACCCCA CAGCAGCAAC AACAACAACA ACAGCAATTT 
CACCATGAGG CCAACTTGTC CAACCTCTCG GCCATGAGTT CAAACACCTC GATTAGCGAG 
GATTCCTGCC AGACGATCAT CACCACTTGC GCACAGGTGC ACTCGGAGCA GAGTCCACTA 
AAGGATCTGC AAATGCTGGT CGCTCAGGAC GAGCTGCCAC TGCCGCCGCC AATGGAACTT 
CCTCCGTACC CAAGTCCACC GCACTCCTCC TGCCATTCGC GGCAGGCTAG CGAAGATTTC 
CCGCCGCCAC CACCGCCGGA ACTGGATCTG GAGCCACTCA ACCAGCAACT GAGCCAGCTG 
CAGGCACTGG AGGCGGCCAG TAAGCAGCAG CGCCAGCAAC TCGAGTCCCT CGAGGGCACC 
ACCAGCATTT TGGCCCAGCT GCAGCAGCGC CAGCACCTGC TCAAGCTGAG AAAGGAGCAG 
GCCAATCACC CGGGAGCAGC CACCACGGAT GCCACCTGGC TGAAGGAGCT GCAGGCGAAA 
CAGGCAAACG ATTTGAGAGC CATGCAACGT AAGCTGGAGG CCTCGTCGCC ATCAAGTGTC 
AGGGACTTGA CTCATCGTTT CGAGCAAACC TCCATTCGAT CCTACGCCTC GCAGGAGCTA 
TTGTCCCAGC CGGGACAGAG TCAGCAGCTG GGGCAGCTGC GCACCCAGAT GAATGGGCTG 
CCAAACGGCA ATGCAAAAAT GGATGTAGAT GAGGTGGACG CTATGCCAGC GGTGAGAAAC 
TCTCTGCCCG CCGCACTCAG CGCCCACCAA TTGCTGCCCA AGCCTAAGTA CGAAATGACC 
CAATCGCAGA TTGCAGAGGA GATTCGCGAA GTGGAGTTGC TGAACACGAT GGTACAGCAG 
ACACTGAATC AGAACGGAGC TGCCGCACTA CCTAAGCGGG TAAAGAAAAA GAGCGTCTCC 
TTTTGCGACC AGGTGATCCT GGTTGCCACA GCCGGAAACG AGGAGGACGA TGACTTTATA 
CCCAATCCCA TACTGGAGCG GGTCCTGCGG ACTGCCCAGC ATCCCAACGA AAAAGTCACT 
GCTCAAATGA TCCAGCAGCA GCAGCAGAAC ATGATGCGAT TGCAAACAGA AGCGCAGCCG 
CGGCAACAGC AGGTGCAGCA GCTGCAACAA CCATCACCCA TGTTGGGCAG ACCAGCAGGA 
GCCACAGCAG ATATGCAGCG ATACGTCCAG AGGATGCAGC AACAACAGCA GCAGCAGCAA 
CAACAGCAGC AGCAACAACA GCAGCAGCAG CAACAACAGC AGCAACAACA GCAGCAGCAA 
CAGCAGCAGC AGCAGCAACA CCAGGAGTTG TACCGCCAGC AGCAGCTACA TCGCACTAGT 
ATGAGCGGCA TAGCCAGCCA GCAACACTTG CAGCAATTGG ATGCACAGTA CACCACCATG 
CCGCGTCCCT TGCATCATTC ACAGCTTGGT AGCTACTCTG TGACCTTGCA GAAACACCAG 
CAACTTCAGC AGCAACAGCA ACAGCAGCAA CAGCAGCAGG CCCCGCAGCA GCTCAGCTTG 
GGCTACTTTA GCAATGGCAG TGTTGCGACT TCAGAGCAAG TCCCGCTGCC TTCGCCCTAC 
CAGCGGGTGC CCCTGCCACA TGGATACCAG CCTGCAGCAG CAGGACCCTA CTACCCGCTA 
CCCAATGCCC AGCAACATCT GCAGCAGCAA CAGCTGATGG CTAAGCCCGT GCAGAAAAAG 
GTGAGCTTTG AGCCAGGAAC TAAAGGCGAA GCCGATTGCT TGCCCCCACC ACCGCCGCCG 
CCGCAAAAGA ACGGCTTGCC CGAGACGGGA TCTGCAGGAT CAGGACACCC TGGAGCAGCA 
CCCGGGGCAA GTGGAGCGGG CGGCACCACA TCGGCCGGCA TCACCGCCAT TCCCACCCGC 
GTCTACAACA ATGCCATTGT AAAGGCTTCG GCCAAAGCCG TTGAGTGCAA TTTGTGCCGC 
AAGCGGCATG TGATCGCACC GGCGGTATAT TGCACCAACT GCGAGTACTA CCTGCAGATG 
CTGAACCAGC GCAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001096877.1
CDS	348..5645
Translation	MIKFRYKRKEPTNVVGVAAPTAGAAALGSGASSTPGTSPTSPVSVPVPVQAHHPHPQLGHLISNNGGTLPRSSPRKLILDGADKSATLTRQQSKQKSVSALAKVASSVELAVMGYNSTGNGNSNGHINSAGNSERDRCINAVIELFQQLQTSSEPALCPEPLRRALASGPLAGRRFPLGCLGDAAECFELLLHRVHSHISPDDGDSCESSACIAHRRFAMRVIEQSVCKCGANSEQLPFTQMVHYVSASALTSQKSLALQSHQQLSFGQLLRAAGNMGDIRDCPNTCGAKIGICRALLNRPEVVSIGIVWDSERPAADQVHAVLKAVGTSLRLGDVFHQVSEPRWAQQTQHELVGIVSYYGKHYTTFFFHTKLKVWVYFDDANVKEVGPSWEGVVDKCSRGRYQPLLLLYAVPQQPTVGNGVGIGHVDVAMPASAPSIAGSVVRRAVTPSPEKPSLGNTRRAITPTPLRTPPNDYQNLSVIQKNIFPSNLAMGTDETDAYISRKTVEHVLSAQYQNLSVIQDKIGGGNGVGVGQQSVDKDGGFLSRKPIEAVLSAQLARRQHLQLQRSHSAESSSGHASSNGSSPPSDGLTMPEHLNQPRRRDSGNWSGDRNSASSASSTTLDNPYLYMVAKRGVAAVPQSPTRHGLPYDPGYDSFSLSSTDSYPPKHALNPQLAKIPEAAMGVVPNGGAMPHGHGNGQLALSGDCEKLCHEADQLLEKSRLVEESHDLETALVLCNAAAGRARAAMDAPYSNPHTMTFARMKHNTCVMRARSLHRRILVEKGAETEAMPELKHMREGSTSSVKHVRQNSKDRTLEKEQQQLQFQQIQQIQQIHQIQQQIPASIEIYATLPKRKSPLKALASASACDDNAIEYEQEKQLATSPGKPERESRSLFGRKDKEKEKRSRSEDRNKLTREFSLTETLLVNAKDTLKKHKEDKDEKKEKDKSGKKQHKIRRKLLMGGLIRRKNRSMPDLTEAVDDSTANAGITHHPHYPAQGVTTAGTLLGTSVDDSAVGLGKHGHGMSGYISEGHFDYSGSGSASNSGAGSNPNPNLERSKLMRKSFHGSGRQLTMVPKVAPPPPMRTSSTLTPQQQQQQQQQFHHEANLSNLSAMSSNTSISEDSCQTIITTCAQVHSEQSPLKDLQMLVAQDELPLPPPMELPPYPSPPHSSCHSRQASEDFPPPPPPELDLEPLNQQLSQLQALEAASKQQRQQLESLEGTTSILAQLQQRQHLLKLRKEQANHPGAATTDATWLKELQAKQANDLRAMQRKLEASSPSSVRDLTHRFEQTSIRSYASQELLSQPGQSQQLGQLRTQMNGLPNGNAKMDVDEVDAMPAVRNSLPAALSAHQLLPKPKYEMTQSQIAEEIREVELLNTMVQQTLNQNGAAALPKRVKKKSVSFCDQVILVATAGNEEDDDFIPNPILERVLRTAQHPNEKVTAQMIQQQQQNMMRLQTEAQPRQQQVQQLQQPSPMLGRPAGATADMQRYVQRMQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQHQELYRQQQLHRTSMSGIASQQHLQQLDAQYTTMPRPLHHSQLGSYSVTLQKHQQLQQQQQQQQQQQAPQQLSLGYFSNGSVATSEQVPLPSPYQRVPLPHGYQPAAAGPYYPLPNAQQHLQQQQLMAKPVQKKVSFEPGTKGEADCLPPPPPPPQKNGLPETGSAGSGHPGAAPGASGAGGTTSAGITAIPTRVYNNAIVKASAKAVECNLCRKRHVIAPAVYCTNCEYYLQMLNQRR

Target ORF information:

RefSeq Version	NM_001103407.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster echinus (ec), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103407.3

ORF Insert Sequence:

ATGATCAAGT TCCGCTACAA AAGGAAAGAA CCCACGAATG TGGTGGGCGT AGCAGCCCCA 
ACTGCAGGAG CAGCAGCATT GGGATCAGGA GCATCCAGCA CCCCGGGCAC ATCCCCAACA 
TCACCTGTTT CCGTTCCCGT TCCCGTTCAA GCCCACCATC CCCATCCCCA GTTGGGGCAC 
CTGATCAGCA ACAATGGAGG CACCCTGCCA AGGAGTAGTC CCCGCAAATT AATCCTGGAT 
GGAGCCGACA AGTCGGCCAC ATTGACCCGC CAGCAGTCGA AGCAGAAGTC GGTCAGTGCC 
TTGGCCAAAG TGGCCAGCAG CGTGGAATTG GCCGTAATGG GCTATAATAG CACTGGCAAT 
GGCAATAGCA ATGGTCATAT CAACAGCGCG GGAAATTCGG AGAGGGATCG GTGCATCAAT 
GCGGTTATCG AACTTTTCCA GCAGCTGCAG ACCAGTTCTG AGCCCGCCTT GTGCCCGGAG 
CCACTGCGTC GGGCTTTGGC CAGTGGTCCA TTGGCTGGAC GGCGATTCCC ACTCGGCTGC 
TTGGGCGATG CAGCCGAATG CTTCGAGCTC CTTCTCCACC GCGTCCACTC GCACATTTCG 
CCGGATGACG GCGACTCCTG CGAATCCTCC GCCTGCATTG CCCACCGAAG ATTCGCCATG 
CGCGTCATCG AGCAGAGCGT CTGCAAGTGC GGAGCCAATT CGGAGCAGCT GCCCTTCACC 
CAGATGGTGC ACTACGTCTC TGCCTCGGCT CTGACCTCCC AGAAAAGTCT GGCACTGCAG 
TCCCATCAGC AACTGAGTTT TGGCCAGCTT TTAAGGGCCG CCGGCAACAT GGGCGACATA 
CGCGATTGTC CGAACACCTG CGGCGCCAAG ATTGGAATTT GTCGTGCGCT GCTTAACCGA 
CCAGAAGTGG TGTCCATTGG CATCGTTTGG GACTCTGAGC GACCGGCGGC GGATCAGGTT 
CATGCCGTCC TAAAGGCAGT GGGCACCAGC CTGCGTCTGG GTGACGTTTT TCACCAGGTC 
AGCGAGCCAC GATGGGCCCA GCAGACCCAG CACGAGCTGG TTGGCATCGT TTCCTACTAC 
GGCAAACACT ACACCACCTT CTTCTTCCAC ACGAAGCTCA AGGTGTGGGT CTACTTCGAT 
GATGCGAACG TTAAGGAGGT GGGGCCCAGT TGGGAGGGCG TGGTGGACAA ATGCAGCCGG 
GGAAGATATC AGCCACTCCT GCTGCTGTAC GCCGTTCCCC AGCAGCCTAC GGTGGGCAAC 
GGCGTAGGTA TCGGTCACGT GGATGTGGCC ATGCCGGCGA GTGCTCCATC GATAGCTGGC 
TCAGTGGTTC GCAGGGCAGT TACGCCCAGT CCGGAGAAGC CCAGCTTGGG CAACACAAGG 
AGGGCCATCA CCCCCACTCC GCTGAGGACA CCACCCAACG ACTATCAGAA CCTGAGTGTG 
ATTCAAAAGA ATATCTTTCC GAGCAATCTG GCAATGGGTA CGGATGAAAC GGATGCCTAC 
ATTAGCCGCA AGACGGTTGA ACATGTGCTG AGTGCCCAGT ACCAGAATTT GAGCGTAATC 
CAGGACAAGA TTGGAGGCGG CAATGGAGTC GGTGTCGGCC AACAGAGTGT GGATAAGGAT 
GGAGGCTTTC TGAGCCGAAA GCCCATTGAG GCAGTGCTGA GTGCCCAACT GGCGCGGAGG 
CAGCACCTCC AGCTTCAACG CAGCCACAGT GCGGAGTCAA GCTCCGGCCA CGCCTCCTCC 
AACGGCAGTT CTCCGCCCAG CGATGGACTG ACCATGCCGG AGCACCTGAA TCAGCCACGT 
CGTCGCGACT CGGGCAATTG GTCAGGCGAT CGAAACAGCG CTAGTTCGGC CAGTTCCACG 
ACGCTGGATA ATCCCTACCT GTACATGGTG GCCAAGCGGG GTGTGGCAGC AGTGCCACAG 
AGCCCCACGC GACATGGTTT GCCATATGAC CCGGGCTACG ATTCGTTTTC GCTAAGCTCC 
ACGGACTCAT ATCCGCCGAA GCATGCGCTA AATCCACAGC TGGCTAAAAT CCCGGAAGCA 
GCCATGGGCG TCGTTCCAAA CGGAGGAGCA ATGCCCCATG GACATGGCAA CGGACAGCTG 
GCCTTGTCTG GGGACTGCGA GAAGCTGTGC CACGAGGCGG ACCAGTTGCT GGAAAAGTCC 
CGACTGGTGG AGGAGTCACA TGATCTGGAA ACAGCCCTGG TGCTCTGCAA TGCGGCTGCT 
GGGCGGGCAC GAGCCGCTAT GGATGCCCCG TATAGCAATC CGCACACGAT GACCTTTGCC 
CGGATGAAGC ACAATACTTG CGTGATGCGG GCCCGTAGTC TGCACCGGCG GATTCTGGTG 
GAGAAGGGAG CTGAAACAGA GGCTATGCCC GAACTGAAGC ACATGCGCGA AGGAAGCACG 
AGTAGTGTGA AGCATGTGCG CCAGAATAGC AAGGATCGCA CGTTAGAGAA GGAGCAGCAG 
CAGCTGCAGT TCCAGCAAAT ACAGCAGATC CAGCAGATTC ATCAGATCCA GCAGCAGATC 
CCAGCCAGCA TCGAAATATA TGCCACGCTC CCGAAGCGCA AGAGTCCTCT GAAGGCCTTG 
GCCTCTGCGT CTGCGTGCGA TGACAACGCA ATCGAGTACG AGCAGGAGAA GCAACTGGCC 
ACCAGTCCGG GAAAACCGGA GCGCGAGAGT CGCTCGCTGT TCGGGCGCAA GGACAAAGAG 
AAGGAGAAGC GGAGTCGCAG CGAGGATCGC AACAAGCTAA CCCGTGAGTT CTCGCTCACC 
GAAACGCTTC TAGTGAATGC CAAGGATACG CTGAAGAAGC ATAAGGAGGA CAAGGACGAG 
AAGAAGGAGA AGGACAAGAG CGGCAAAAAG CAGCACAAAA TCAGACGCAA GCTGTTAATG 
GGCGGGCTCA TTCGTCGCAA GAATCGCTCC ATGCCGGATC TCACAGAGGC GGTGGATGAC 
TCGACGGCCA ATGCCGGCAT AACGCACCAT CCACATTATC CTGCACAGGG CGTAACTACG 
GCGGGCACTC TCTTGGGTAC CTCCGTAGAC GACAGTGCCG TTGGATTGGG CAAACACGGC 
CACGGCATGA GCGGCTACAT CTCTGAGGGA CACTTCGACT ACTCGGGTTC CGGGTCGGCC 
TCAAATTCGG GAGCCGGCAG CAATCCAAAT CCGAATCTGG AGAGGAGCAA GCTGATGCGC 
AAGAGCTTCC ACGGCAGCGG CAGGCAGTTG ACAATGGTAC CGAAAGTGGC TCCACCGCCG 
CCCATGCGAA CCAGCTCCAC TTTGACCCCA CAGCAGCAAC AACAACAACA ACAGCAATTT 
CACCATGAGG CCAACTTGTC CAACCTCTCG GCCATGAGTT CAAACACCTC GATTAGCGAG 
GATTCCTGCC AGACGATCAT CACCACTTGC GCACAGGTGC ACTCGGAGCA GAGTCCACTA 
AAGGATCTGC AAATGCTGGT CGCTCAGGAC GAGCTGCCAC TGCCGCCGCC AATGGAACTT 
CCTCCGTACC CAAGTCCACC GCACTCCTCC TGCCATTCGC GGCAGGCTAG CGAAGATTTC 
CCGCCGCCAC CACCGCCGGA ACTGGATCTG GAGCCACTCA ACCAGCAACT GAGCCAGCTG 
CAGGCACTGG AGGCGGCCAG TAAGCAGCAG CGCCAGCAAC TCGAGTCCCT CGAGGGCACC 
ACCAGCATTT TGGCCCAGCT GCAGCAGCGC CAGCACCTGC TCAAGCTGAG AAAGGAGCAG 
GCCAATCACC CGGGAGCAGC CACCACGGAT GCCACCTGGC TGAAGGAGCT GCAGGCGAAA 
CAGGCAAACG ATTTGAGAGC CATGCAACGT AAGCTGGAGG CCTCGTCGCC ATCAAGTGTC 
AGGGACTTGA CTCATCGTTT CGAGCAAACC TCCATTCGAT CCTACGCCTC GCAGGAGCTA 
TTGTCCCAGC CGGGACAGAG TCAGCAGCTG GGGCAGCTGC GCACCCAGAT GAATGGGCTG 
CCAAACGGCA ATGCAAAAAT GGATGTAGAT GAGGTGGACG CTATGCCAGC GGTGAGAAAC 
TCTCTGCCCG CCGCACTCAG CGCCCACCAA TTGCTGCCCA AGCCTAAGTA CGAAATGACC 
CAATCGCAGA TTGCAGAGGA GATTCGCGAA GTGGAGTTGC TGAACACGAT GGTACAGCAG 
ACACTGAATC AGAACGGAGC TGCCGCACTA CCTAAGCGGG TAAAGAAAAA GAGCGTCTCC 
TTTTGCGACC AGGTGATCCT GGTTGCCACA GCCGGAAACG AGGAGGACGA TGACTTTATA 
CCCAATCCCA TACTGGAGCG GGTCCTGCGG ACTGCCCAGC ATCCCAACGA AAAAGTCACT 
GCTCAAATGA TCCAGCAGCA GCAGCAGAAC ATGATGCGAT TGCAAACAGA AGCGCAGCCG 
CGGCAACAGC AGGTGCAGCA GCTGCAACAA CCATCACCCA TGTTGGGCAG ACCAGCAGGA 
GCCACAGCAG ATATGCAGCG ATACGTCCAG AGGATGCAGC AACAACAGCA GCAGCAGCAA 
CAACAGCAGC AGCAACAACA GCAGCAGCAG CAACAACAGC AGCAACAACA GCAGCAGCAA 
CAGCAGCAGC AGCAGCAACA CCAGGAGTTG TACCGCCAGC AGCAGCTACA TCGCACTAGT 
ATGAGCGGCA TAGCCAGCCA GCAACACTTG CAGCAATTGG ATGCACAGTA CACCACCATG 
CCGCGTCCCT TGCATCATTC ACAGCTTGGT AGCTACTCTG TGACCTTGCA GAAACACCAG 
CAACTTCAGC AGCAACAGCA ACAGCAGCAA CAGCAGCAGG CCCCGCAGCA GCTCAGCTTG 
GGCTACTTTA GCAATGGCAG TGTTGCGACT TCAGAGCAAG TCCCGCTGCC TTCGCCCTAC 
CAGCGGGTGC CCCTGCCACA TGGATACCAG CCTGCAGCAG CAGGACCCTA CTACCCGCTA 
CCCAATGCCC AGCAACATCT GCAGCAGCAA CAGCTGATGG CTAAGCCCGT GCAGAAAAAG 
GTGAGCTTTG AGCCAGGAAC TAAAGGCGAA GCCGATTGCT TGCCCCCACC ACCGCCGCCG 
CCGCAAAAGA ACGGCTTGCC CGAGACGGGA TCTGCAGGAT CAGGACACCC TGGAGCAGCA 
CCCGGGGCAA GTGGAGCGGG CGGCACCACA TCGGCCGGCA TCACCGCCAT TCCCACCCGC 
GTCTACAACA ATGCCATTGT AAAGGCTTCG GCCAAAGCCG TTGAGTGCAA TTTGTGCCGC 
AAGCGGCATG TGATCGCACC GGCGGTATAT TGCACCAACT GCGAGTACTA CCTGCAGATG 
CTGAACCAGC GCAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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ec cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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