CG12773 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	CG12773
Entrez Gene ID	31082
Full Name	CG12773 gene product from transcript CG12773-RC
Synonyms	Dmel\CG12773,EG:8D8.3
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 1F4-1F4

mRNA and Protein(s)

mRNA	Protein	Name
NM_130549.3	NP_569905.1	uncharacterized protein, isoform A
NM_001272202.1	NP_001259131.1	uncharacterized protein, isoform B
NM_001297844.1	NP_001284773.1	uncharacterized protein, isoform C

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SLC12A8	XP_005639642.1
Homo sapiens (human)	SLC12A8	NP_001182412.1
Gallus gallus (chicken)	SLC12A8	XP_003641672.1
Xenopus tropicalis (tropical clawed frog)	LOC100488646	XP_002943856.1
Mus musculus (house mouse)	Slc12a8	NP_599012.2
Drosophila melanogaster (fruit fly)	CG12773	NP_569905.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006249	XP_316315.4
Pan troglodytes (chimpanzee)	SLC12A8	XP_516709.4
Bos taurus (cattle)	SLC12A8	XP_002684861.2
	SLC12A8	XP_002802725.1
Rattus norvegicus (Norway rat)	Slc12a8	NP_705889.1
Danio rerio (zebrafish)	slc12a8	NP_001121749.1
Caenorhabditis elegans (roundworm)	F10E7.9	NP_495469.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CG12773 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CG12773 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa00460	NM_001297844.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant C (CG12773), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa00460	NM_130549.3 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant A (CG12773), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa02959	NM_001272202.1 Latest version!	Drosophila melanogaster uncharacterized protein, transcript variant B (CG12773), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CG12773 ( NM_130549.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_569905.1 Drosophila melanogaster uncharacterized protein, transcript variant A (CG12773), mRNA.

Price & Availability↑

CloneID	OFa00460
Clone ID Related Accession (Same CDS sequence)	NM_130549.3 , NM_001297844.1
Accession Version	NM_130549.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_130549.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTAATA GCTCTGAGGG CAATGGCAAC GGCGGCCGAC GCACTGTCGA CTGGCAGAGA 
TTTGGACTTG GCGGCGACGA GGACGGGGAT GTGGCGGTGA CCAGCTTTCG CCAGCGGACC 
AACAACAACA CCGGCGGATT TGTGGATTTA GGTAACGAGT ACACGTACGC GGCTGGTGGC 
CACCATGCGT CCGGGGCCAA CGAGATCTTT GCGGGCGAGC AGGGCGACAA GCCGTGGTGG 
CGCTCGAACT TCTTCATCTC CCAGCCCGTG CTATTCGGCA CCTGGGACGG AGTGTTCACC 
TCCTGCCTAA TCAACGTGTT TGGAGTGATC GTGTTCCTGC GATCCGGATG GATCGTCGCA 
CAGGCAGGCA TTCTGAACGC GGTGCTGATC ATCTTTTGCA CGGTGGTCAT AGCTCTCGTC 
AGCGTCCTCT CGGCAATCGG TATCTGCGAG CGGTGCCGTG TGGAAAGCGG TGGCGTGTAC 
TTTCTCATAG CCCACACGCT TGGTTCCCGA TTCGGAGGCG CCTTGGGTTT GCTCTACTGC 
TTTGGCCAGG CGGTGGGATG CGCCCTTAAC GTAATGGGAT TTGGCGAATC AATGGCTGGA 
CTAGTGGGAC TCGAGGGCAG CAAGTGGGCC ATCCGCGGGT TCGCTACGGC AGCGGTGCTC 
CTGTTGGGCT GCATCAACGT GGCCGGCGTC AAGTGGGTCA TCAAGCTTCA GTTTATACTG 
CTCATGATAC TGCTGATCTC GGCGCTGGAC TTCATGGTGG GCAGCTTCAC TAGTGAGGCC 
AGTGGCGGCT TTAATGGCTG GGCGAGCGGA AACTTCGTGG AGAACCTATG GCCAAAGTAC 
GACGACGGCT ACTCCTGGTT CCGTGTGTTC GGCGTCTTCT TTCCCACCGT CACCGGAGTG 
CTGTCGGGCA TTAACATGAG CGGCGACTTG CGCGCGCCGT CCACGGACAT TCCCAATGGC 
ACCCTGGCCG CCTTTGGCAC CTCCACGTTC CTGTACTTGG TGTTCGTCCT GTTTTTGGGG 
GCCACCTGCC AACGAAGCTT CCTGTATACT GACTACATGA TCTCTGTCAA GGTGTCTGCC 
GTGCATTTCC TGCTGTTGGC CGGCATTTAT GTGTCCAGCA TGTCCTCCTG CCTGGGCGCC 
ATGTACGGCA CTCCGCGCGT CCTTCAGAGC ATCGCCAAAG AGAGCGTCAT TCCGGGCATC 
GATATCCTGG GAAAGGGTCG TGGTCCCAAC AAAGTGCCTT TATACGCAAT GGCCATCGTG 
GCCCTAGTCA CTGTCACCTT CATTATCGTG GGAGACATCA ACTTCTTGGC GCCGATCGTG 
ACCATGCCCT TCCTGCTCAC ATATGCCTGT ATCGACTACG CCTACTTTGC GCTGGCCCAG 
ACCTTCGACA TTCAGGAGCA GCGCGAGGAG CGTTTCCGCA TCCAGGCGTC GAGTCCCAGC 
TACGAGACGC GCCGTTACGG CAGTGTTTCG GACACGGGCA ACGACCTGGA CCTCCTGTTC 
CCTGATCGGG TGCGCCACAA GAACCTGCAG AGTCCGCAGA ACTCACCGCT GCACCAAACA 
GTGCCCCTGG AGTTTGACAA CGAGGGTCCC AGCACCTCCA ACCAGGCCCA GTCTTCGGAA 
CGGAGAACGG GGCACGAGGC GGACACCACA CTGGCCGTGG AGCAGGCAGT CGCGCAGGCC 
GGCGTCGAAC AGGACCAGGG CGACGAGGCC GAACCGATAG CCCCGATCCG TCCGCCCATC 
CACTCCAAGA CGAAAAACTG GTACTCCGGC TACTGTAATC GCTGGGCCTC TCTGCTGGGG 
GCCTTCACTA AACTGCTGGT TATGCTGCTA GTCAACTGGT ACTACGCCCT CACCTGCTTC 
TTGGTGGTGT TCGTCGTATG GTTCTATGTG GGCACAGCCA ATCCGGCCGT GAAGCCGGGT 
CTCACTGCGG AGTTTAACTT CTTCGCCTGG CTGAAGAGCG TCATATTCCG GTGCTTCGGC 
AAACGGATGA ACGAGTACGA GCAGATAGTC GTGACACCCT CGTGCCCAGG CGTTGATCTC 
TCACCCACGC AGCTCAACGA GCAGAACGAG GACTTCCGTC CGCGGCCCAA CTACCACCAC 
TCGTCCGTTA TCGAGGGTCG CCTCATAGAT GACATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_569905.1
CDS	494..2632
Translation	MTNSSEGNGNGGRRTVDWQRFGLGGDEDGDVAVTSFRQRTNNNTGGFVDLGNEYTYAAGGHHASGANEIFAGEQGDKPWWRSNFFISQPVLFGTWDGVFTSCLINVFGVIVFLRSGWIVAQAGILNAVLIIFCTVVIALVSVLSAIGICERCRVESGGVYFLIAHTLGSRFGGALGLLYCFGQAVGCALNVMGFGESMAGLVGLEGSKWAIRGFATAAVLLLGCINVAGVKWVIKLQFILLMILLISALDFMVGSFTSEASGGFNGWASGNFVENLWPKYDDGYSWFRVFGVFFPTVTGVLSGINMSGDLRAPSTDIPNGTLAAFGTSTFLYLVFVLFLGATCQRSFLYTDYMISVKVSAVHFLLLAGIYVSSMSSCLGAMYGTPRVLQSIAKESVIPGIDILGKGRGPNKVPLYAMAIVALVTVTFIIVGDINFLAPIVTMPFLLTYACIDYAYFALAQTFDIQEQREERFRIQASSPSYETRRYGSVSDTGNDLDLLFPDRVRHKNLQSPQNSPLHQTVPLEFDNEGPSTSNQAQSSERRTGHEADTTLAVEQAVAQAGVEQDQGDEAEPIAPIRPPIHSKTKNWYSGYCNRWASLLGAFTKLLVMLLVNWYYALTCFLVVFVVWFYVGTANPAVKPGLTAEFNFFAWLKSVIFRCFGKRMNEYEQIVVTPSCPGVDLSPTQLNEQNEDFRPRPNYHHSSVIEGRLIDDI

Target ORF information:

RefSeq Version	NM_130549.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant A (CG12773), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_130549.3

ORF Insert Sequence:

ATGACTAATA GCTCTGAGGG CAATGGCAAC GGCGGCCGAC GCACTGTCGA CTGGCAGAGA 
TTTGGACTTG GCGGCGACGA GGACGGGGAT GTGGCGGTGA CCAGCTTTCG CCAGCGGACC 
AACAACAACA CCGGCGGATT TGTGGATTTA GGTAACGAGT ACACGTACGC GGCTGGTGGC 
CACCATGCGT CCGGGGCCAA CGAGATCTTT GCGGGCGAGC AGGGCGACAA GCCGTGGTGG 
CGCTCGAACT TCTTCATCTC CCAGCCCGTG CTATTCGGCA CCTGGGACGG AGTGTTCACC 
TCCTGCCTAA TCAACGTGTT TGGAGTGATC GTGTTCCTGC GATCCGGATG GATCGTCGCA 
CAGGCAGGCA TTCTGAACGC GGTGCTGATC ATCTTTTGCA CGGTGGTCAT AGCTCTCGTC 
AGCGTCCTCT CGGCAATCGG TATCTGCGAG CGGTGCCGTG TGGAAAGCGG TGGCGTGTAC 
TTTCTCATAG CCCACACGCT TGGTTCCCGA TTCGGAGGCG CCTTGGGTTT GCTCTACTGC 
TTTGGCCAGG CGGTGGGATG CGCCCTTAAC GTAATGGGAT TTGGCGAATC AATGGCTGGA 
CTAGTGGGAC TCGAGGGCAG CAAGTGGGCC ATCCGCGGGT TCGCTACGGC AGCGGTGCTC 
CTGTTGGGCT GCATCAACGT GGCCGGCGTC AAGTGGGTCA TCAAGCTTCA GTTTATACTG 
CTCATGATAC TGCTGATCTC GGCGCTGGAC TTCATGGTGG GCAGCTTCAC TAGTGAGGCC 
AGTGGCGGCT TTAATGGCTG GGCGAGCGGA AACTTCGTGG AGAACCTATG GCCAAAGTAC 
GACGACGGCT ACTCCTGGTT CCGTGTGTTC GGCGTCTTCT TTCCCACCGT CACCGGAGTG 
CTGTCGGGCA TTAACATGAG CGGCGACTTG CGCGCGCCGT CCACGGACAT TCCCAATGGC 
ACCCTGGCCG CCTTTGGCAC CTCCACGTTC CTGTACTTGG TGTTCGTCCT GTTTTTGGGG 
GCCACCTGCC AACGAAGCTT CCTGTATACT GACTACATGA TCTCTGTCAA GGTGTCTGCC 
GTGCATTTCC TGCTGTTGGC CGGCATTTAT GTGTCCAGCA TGTCCTCCTG CCTGGGCGCC 
ATGTACGGCA CTCCGCGCGT CCTTCAGAGC ATCGCCAAAG AGAGCGTCAT TCCGGGCATC 
GATATCCTGG GAAAGGGTCG TGGTCCCAAC AAAGTGCCTT TATACGCAAT GGCCATCGTG 
GCCCTAGTCA CTGTCACCTT CATTATCGTG GGAGACATCA ACTTCTTGGC GCCGATCGTG 
ACCATGCCCT TCCTGCTCAC ATATGCCTGT ATCGACTACG CCTACTTTGC GCTGGCCCAG 
ACCTTCGACA TTCAGGAGCA GCGCGAGGAG CGTTTCCGCA TCCAGGCGTC GAGTCCCAGC 
TACGAGACGC GCCGTTACGG CAGTGTTTCG GACACGGGCA ACGACCTGGA CCTCCTGTTC 
CCTGATCGGG TGCGCCACAA GAACCTGCAG AGTCCGCAGA ACTCACCGCT GCACCAAACA 
GTGCCCCTGG AGTTTGACAA CGAGGGTCCC AGCACCTCCA ACCAGGCCCA GTCTTCGGAA 
CGGAGAACGG GGCACGAGGC GGACACCACA CTGGCCGTGG AGCAGGCAGT CGCGCAGGCC 
GGCGTCGAAC AGGACCAGGG CGACGAGGCC GAACCGATAG CCCCGATCCG TCCGCCCATC 
CACTCCAAGA CGAAAAACTG GTACTCCGGC TACTGTAATC GCTGGGCCTC TCTGCTGGGG 
GCCTTCACTA AACTGCTGGT TATGCTGCTA GTCAACTGGT ACTACGCCCT CACCTGCTTC 
TTGGTGGTGT TCGTCGTATG GTTCTATGTG GGCACAGCCA ATCCGGCCGT GAAGCCGGGT 
CTCACTGCGG AGTTTAACTT CTTCGCCTGG CTGAAGAGCG TCATATTCCG GTGCTTCGGC 
AAACGGATGA ACGAGTACGA GCAGATAGTC GTGACACCCT CGTGCCCAGG CGTTGATCTC 
TCACCCACGC AGCTCAACGA GCAGAACGAG GACTTCCGTC CGCGGCCCAA CTACCACCAC 
TCGTCCGTTA TCGAGGGTCG CCTCATAGAT GACATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG12773 ( NM_001297844.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284773.1 Drosophila melanogaster uncharacterized protein, transcript variant C (CG12773), mRNA.

Price & Availability↑

CloneID	OFa00460
Clone ID Related Accession (Same CDS sequence)	NM_130549.3 , NM_001297844.1
Accession Version	NM_001297844.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTAATA GCTCTGAGGG CAATGGCAAC GGCGGCCGAC GCACTGTCGA CTGGCAGAGA 
TTTGGACTTG GCGGCGACGA GGACGGGGAT GTGGCGGTGA CCAGCTTTCG CCAGCGGACC 
AACAACAACA CCGGCGGATT TGTGGATTTA GGTAACGAGT ACACGTACGC GGCTGGTGGC 
CACCATGCGT CCGGGGCCAA CGAGATCTTT GCGGGCGAGC AGGGCGACAA GCCGTGGTGG 
CGCTCGAACT TCTTCATCTC CCAGCCCGTG CTATTCGGCA CCTGGGACGG AGTGTTCACC 
TCCTGCCTAA TCAACGTGTT TGGAGTGATC GTGTTCCTGC GATCCGGATG GATCGTCGCA 
CAGGCAGGCA TTCTGAACGC GGTGCTGATC ATCTTTTGCA CGGTGGTCAT AGCTCTCGTC 
AGCGTCCTCT CGGCAATCGG TATCTGCGAG CGGTGCCGTG TGGAAAGCGG TGGCGTGTAC 
TTTCTCATAG CCCACACGCT TGGTTCCCGA TTCGGAGGCG CCTTGGGTTT GCTCTACTGC 
TTTGGCCAGG CGGTGGGATG CGCCCTTAAC GTAATGGGAT TTGGCGAATC AATGGCTGGA 
CTAGTGGGAC TCGAGGGCAG CAAGTGGGCC ATCCGCGGGT TCGCTACGGC AGCGGTGCTC 
CTGTTGGGCT GCATCAACGT GGCCGGCGTC AAGTGGGTCA TCAAGCTTCA GTTTATACTG 
CTCATGATAC TGCTGATCTC GGCGCTGGAC TTCATGGTGG GCAGCTTCAC TAGTGAGGCC 
AGTGGCGGCT TTAATGGCTG GGCGAGCGGA AACTTCGTGG AGAACCTATG GCCAAAGTAC 
GACGACGGCT ACTCCTGGTT CCGTGTGTTC GGCGTCTTCT TTCCCACCGT CACCGGAGTG 
CTGTCGGGCA TTAACATGAG CGGCGACTTG CGCGCGCCGT CCACGGACAT TCCCAATGGC 
ACCCTGGCCG CCTTTGGCAC CTCCACGTTC CTGTACTTGG TGTTCGTCCT GTTTTTGGGG 
GCCACCTGCC AACGAAGCTT CCTGTATACT GACTACATGA TCTCTGTCAA GGTGTCTGCC 
GTGCATTTCC TGCTGTTGGC CGGCATTTAT GTGTCCAGCA TGTCCTCCTG CCTGGGCGCC 
ATGTACGGCA CTCCGCGCGT CCTTCAGAGC ATCGCCAAAG AGAGCGTCAT TCCGGGCATC 
GATATCCTGG GAAAGGGTCG TGGTCCCAAC AAAGTGCCTT TATACGCAAT GGCCATCGTG 
GCCCTAGTCA CTGTCACCTT CATTATCGTG GGAGACATCA ACTTCTTGGC GCCGATCGTG 
ACCATGCCCT TCCTGCTCAC ATATGCCTGT ATCGACTACG CCTACTTTGC GCTGGCCCAG 
ACCTTCGACA TTCAGGAGCA GCGCGAGGAG CGTTTCCGCA TCCAGGCGTC GAGTCCCAGC 
TACGAGACGC GCCGTTACGG CAGTGTTTCG GACACGGGCA ACGACCTGGA CCTCCTGTTC 
CCTGATCGGG TGCGCCACAA GAACCTGCAG AGTCCGCAGA ACTCACCGCT GCACCAAACA 
GTGCCCCTGG AGTTTGACAA CGAGGGTCCC AGCACCTCCA ACCAGGCCCA GTCTTCGGAA 
CGGAGAACGG GGCACGAGGC GGACACCACA CTGGCCGTGG AGCAGGCAGT CGCGCAGGCC 
GGCGTCGAAC AGGACCAGGG CGACGAGGCC GAACCGATAG CCCCGATCCG TCCGCCCATC 
CACTCCAAGA CGAAAAACTG GTACTCCGGC TACTGTAATC GCTGGGCCTC TCTGCTGGGG 
GCCTTCACTA AACTGCTGGT TATGCTGCTA GTCAACTGGT ACTACGCCCT CACCTGCTTC 
TTGGTGGTGT TCGTCGTATG GTTCTATGTG GGCACAGCCA ATCCGGCCGT GAAGCCGGGT 
CTCACTGCGG AGTTTAACTT CTTCGCCTGG CTGAAGAGCG TCATATTCCG GTGCTTCGGC 
AAACGGATGA ACGAGTACGA GCAGATAGTC GTGACACCCT CGTGCCCAGG CGTTGATCTC 
TCACCCACGC AGCTCAACGA GCAGAACGAG GACTTCCGTC CGCGGCCCAA CTACCACCAC 
TCGTCCGTTA TCGAGGGTCG CCTCATAGAT GACATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284773.1
CDS	494..2632
Translation	MTNSSEGNGNGGRRTVDWQRFGLGGDEDGDVAVTSFRQRTNNNTGGFVDLGNEYTYAAGGHHASGANEIFAGEQGDKPWWRSNFFISQPVLFGTWDGVFTSCLINVFGVIVFLRSGWIVAQAGILNAVLIIFCTVVIALVSVLSAIGICERCRVESGGVYFLIAHTLGSRFGGALGLLYCFGQAVGCALNVMGFGESMAGLVGLEGSKWAIRGFATAAVLLLGCINVAGVKWVIKLQFILLMILLISALDFMVGSFTSEASGGFNGWASGNFVENLWPKYDDGYSWFRVFGVFFPTVTGVLSGINMSGDLRAPSTDIPNGTLAAFGTSTFLYLVFVLFLGATCQRSFLYTDYMISVKVSAVHFLLLAGIYVSSMSSCLGAMYGTPRVLQSIAKESVIPGIDILGKGRGPNKVPLYAMAIVALVTVTFIIVGDINFLAPIVTMPFLLTYACIDYAYFALAQTFDIQEQREERFRIQASSPSYETRRYGSVSDTGNDLDLLFPDRVRHKNLQSPQNSPLHQTVPLEFDNEGPSTSNQAQSSERRTGHEADTTLAVEQAVAQAGVEQDQGDEAEPIAPIRPPIHSKTKNWYSGYCNRWASLLGAFTKLLVMLLVNWYYALTCFLVVFVVWFYVGTANPAVKPGLTAEFNFFAWLKSVIFRCFGKRMNEYEQIVVTPSCPGVDLSPTQLNEQNEDFRPRPNYHHSSVIEGRLIDDI

Target ORF information:

RefSeq Version	NM_001297844.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant C (CG12773), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297844.1

ORF Insert Sequence:

ATGACTAATA GCTCTGAGGG CAATGGCAAC GGCGGCCGAC GCACTGTCGA CTGGCAGAGA 
TTTGGACTTG GCGGCGACGA GGACGGGGAT GTGGCGGTGA CCAGCTTTCG CCAGCGGACC 
AACAACAACA CCGGCGGATT TGTGGATTTA GGTAACGAGT ACACGTACGC GGCTGGTGGC 
CACCATGCGT CCGGGGCCAA CGAGATCTTT GCGGGCGAGC AGGGCGACAA GCCGTGGTGG 
CGCTCGAACT TCTTCATCTC CCAGCCCGTG CTATTCGGCA CCTGGGACGG AGTGTTCACC 
TCCTGCCTAA TCAACGTGTT TGGAGTGATC GTGTTCCTGC GATCCGGATG GATCGTCGCA 
CAGGCAGGCA TTCTGAACGC GGTGCTGATC ATCTTTTGCA CGGTGGTCAT AGCTCTCGTC 
AGCGTCCTCT CGGCAATCGG TATCTGCGAG CGGTGCCGTG TGGAAAGCGG TGGCGTGTAC 
TTTCTCATAG CCCACACGCT TGGTTCCCGA TTCGGAGGCG CCTTGGGTTT GCTCTACTGC 
TTTGGCCAGG CGGTGGGATG CGCCCTTAAC GTAATGGGAT TTGGCGAATC AATGGCTGGA 
CTAGTGGGAC TCGAGGGCAG CAAGTGGGCC ATCCGCGGGT TCGCTACGGC AGCGGTGCTC 
CTGTTGGGCT GCATCAACGT GGCCGGCGTC AAGTGGGTCA TCAAGCTTCA GTTTATACTG 
CTCATGATAC TGCTGATCTC GGCGCTGGAC TTCATGGTGG GCAGCTTCAC TAGTGAGGCC 
AGTGGCGGCT TTAATGGCTG GGCGAGCGGA AACTTCGTGG AGAACCTATG GCCAAAGTAC 
GACGACGGCT ACTCCTGGTT CCGTGTGTTC GGCGTCTTCT TTCCCACCGT CACCGGAGTG 
CTGTCGGGCA TTAACATGAG CGGCGACTTG CGCGCGCCGT CCACGGACAT TCCCAATGGC 
ACCCTGGCCG CCTTTGGCAC CTCCACGTTC CTGTACTTGG TGTTCGTCCT GTTTTTGGGG 
GCCACCTGCC AACGAAGCTT CCTGTATACT GACTACATGA TCTCTGTCAA GGTGTCTGCC 
GTGCATTTCC TGCTGTTGGC CGGCATTTAT GTGTCCAGCA TGTCCTCCTG CCTGGGCGCC 
ATGTACGGCA CTCCGCGCGT CCTTCAGAGC ATCGCCAAAG AGAGCGTCAT TCCGGGCATC 
GATATCCTGG GAAAGGGTCG TGGTCCCAAC AAAGTGCCTT TATACGCAAT GGCCATCGTG 
GCCCTAGTCA CTGTCACCTT CATTATCGTG GGAGACATCA ACTTCTTGGC GCCGATCGTG 
ACCATGCCCT TCCTGCTCAC ATATGCCTGT ATCGACTACG CCTACTTTGC GCTGGCCCAG 
ACCTTCGACA TTCAGGAGCA GCGCGAGGAG CGTTTCCGCA TCCAGGCGTC GAGTCCCAGC 
TACGAGACGC GCCGTTACGG CAGTGTTTCG GACACGGGCA ACGACCTGGA CCTCCTGTTC 
CCTGATCGGG TGCGCCACAA GAACCTGCAG AGTCCGCAGA ACTCACCGCT GCACCAAACA 
GTGCCCCTGG AGTTTGACAA CGAGGGTCCC AGCACCTCCA ACCAGGCCCA GTCTTCGGAA 
CGGAGAACGG GGCACGAGGC GGACACCACA CTGGCCGTGG AGCAGGCAGT CGCGCAGGCC 
GGCGTCGAAC AGGACCAGGG CGACGAGGCC GAACCGATAG CCCCGATCCG TCCGCCCATC 
CACTCCAAGA CGAAAAACTG GTACTCCGGC TACTGTAATC GCTGGGCCTC TCTGCTGGGG 
GCCTTCACTA AACTGCTGGT TATGCTGCTA GTCAACTGGT ACTACGCCCT CACCTGCTTC 
TTGGTGGTGT TCGTCGTATG GTTCTATGTG GGCACAGCCA ATCCGGCCGT GAAGCCGGGT 
CTCACTGCGG AGTTTAACTT CTTCGCCTGG CTGAAGAGCG TCATATTCCG GTGCTTCGGC 
AAACGGATGA ACGAGTACGA GCAGATAGTC GTGACACCCT CGTGCCCAGG CGTTGATCTC 
TCACCCACGC AGCTCAACGA GCAGAACGAG GACTTCCGTC CGCGGCCCAA CTACCACCAC 
TCGTCCGTTA TCGAGGGTCG CCTCATAGAT GACATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CG12773 ( NM_001272202.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259131.1 Drosophila melanogaster uncharacterized protein, transcript variant B (CG12773), mRNA.

Price & Availability↑

CloneID	OFa02959
Clone ID Related Accession (Same CDS sequence)	NM_001272202.1
Accession Version	NM_001272202.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	uncharacterized protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACTAATA GCTCTGAGGG CAATGGCAAC GGCGGCCGAC GCACTGTCGA CTGGCAGAGA 
TTTGGACTTG GCGGCGACGA GGACGGGGAT GTGGCGGTGA CCAGCTTTCG CCAGCGGACC 
AACAACAACA CCGGCGGATT TGTGGATTTA GGTAACGAGT ACACGTACGC GGCTGGTGGC 
CACCATGCGT CCGGGGCCAA CGAGATCTTT GCGGGCGAGC AGGGCGACAA GCCGTGGCGC 
TCGAACTTCT TCATCTCCCA GCCCGTGCTA TTCGGCACCT GGGACGGAGT GTTCACCTCC 
TGCCTAATCA ACGTGTTTGG AGTGATCGTG TTCCTGCGAT CCGGATGGAT CGTCGCACAG 
GCAGGCATTC TGAACGCGGT GCTGATCATC TTTTGCACGG TGGTCATAGC TCTCGTCAGC 
GTCCTCTCGG CAATCGGTAT CTGCGAGCGG TGCCGTGTGG AAAGCGGTGG CGTGTACTTT 
CTCATAGCCC ACACGCTTGG TTCCCGATTC GGAGGCGCCT TGGGTTTGCT CTACTGCTTT 
GGCCAGGCGG TGGGATGCGC CCTTAACGTA ATGGGATTTG GCGAATCAAT GGCTGGACTA 
GTGGGACTCG AGGGCAGCAA GTGGGCCATC CGCGGGTTCG CTACGGCAGC GGTGCTCCTG 
TTGGGCTGCA TCAACGTGGC CGGCGTCAAG TGGGTCATCA AGCTTCAGTT TATACTGCTC 
ATGATACTGC TGATCTCGGC GCTGGACTTC ATGGTGGGCA GCTTCACTAG TGAGGCCAGT 
GGCGGCTTTA ATGGCTGGGC GAGCGGAAAC TTCGTGGAGA ACCTATGGCC AAAGTACGAC 
GACGGCTACT CCTGGTTCCG TGTGTTCGGC GTCTTCTTTC CCACCGTCAC CGGAGTGCTG 
TCGGGCATTA ACATGAGCGG CGACTTGCGC GCGCCGTCCA CGGACATTCC CAATGGCACC 
CTGGCCGCCT TTGGCACCTC CACGTTCCTG TACTTGGTGT TCGTCCTGTT TTTGGGGGCC 
ACCTGCCAAC GAAGCTTCCT GTATACTGAC TACATGATCT CTGTCAAGGT GTCTGCCGTG 
CATTTCCTGC TGTTGGCCGG CATTTATGTG TCCAGCATGT CCTCCTGCCT GGGCGCCATG 
TACGGCACTC CGCGCGTCCT TCAGAGCATC GCCAAAGAGA GCGTCATTCC GGGCATCGAT 
ATCCTGGGAA AGGGTCGTGG TCCCAACAAA GTGCCTTTAT ACGCAATGGC CATCGTGGCC 
CTAGTCACTG TCACCTTCAT TATCGTGGGA GACATCAACT TCTTGGCGCC GATCGTGACC 
ATGCCCTTCC TGCTCACATA TGCCTGTATC GACTACGCCT ACTTTGCGCT GGCCCAGACC 
TTCGACATTC AGGAGCAGCG CGAGGAGCGT TTCCGCATCC AGGCGTCGAG TCCCAGCTAC 
GAGACGCGCC GTTACGGCAG TGTTTCGGAC ACGGGCAACG ACCTGGACCT CCTGTTCCCT 
GATCGGGTGC GCCACAAGAA CCTGCAGAGT CCGCAGAACT CACCGCTGCA CCAAACAGTG 
CCCCTGGAGT TTGACAACGA GGGTCCCAGC ACCTCCAACC AGGCCCAGTC TTCGGAACGG 
AGAACGGGGC ACGAGGCGGA CACCACACTG GCCGTGGAGC AGGCAGTCGC GCAGGCCGGC 
GTCGAACAGG ACCAGGGCGA CGAGGCCGAA CCGATAGCCC CGATCCGTCC GCCCATCCAC 
TCCAAGACGA AAAACTGGTA CTCCGGCTAC TGTAATCGCT GGGCCTCTCT GCTGGGGGCC 
TTCACTAAAC TGCTGGTTAT GCTGCTAGTC AACTGGTACT ACGCCCTCAC CTGCTTCTTG 
GTGGTGTTCG TCGTATGGTT CTATGTGGGC ACAGCCAATC CGGCCGTGAA GCCGGGTCTC 
ACTGCGGAGT TTAACTTCTT CGCCTGGCTG AAGAGCGTCA TATTCCGGTG CTTCGGCAAA 
CGGATGAACG AGTACGAGCA GATAGTCGTG ACACCCTCGT GCCCAGGCGT TGATCTCTCA 
CCCACGCAGC TCAACGAGCA GAACGAGGAC TTCCGTCCGC GGCCCAACTA CCACCACTCG 
TCCGTTATCG AGGGTCGCCT CATAGATGAC ATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259131.1
CDS	494..2629
Translation	MTNSSEGNGNGGRRTVDWQRFGLGGDEDGDVAVTSFRQRTNNNTGGFVDLGNEYTYAAGGHHASGANEIFAGEQGDKPWRSNFFISQPVLFGTWDGVFTSCLINVFGVIVFLRSGWIVAQAGILNAVLIIFCTVVIALVSVLSAIGICERCRVESGGVYFLIAHTLGSRFGGALGLLYCFGQAVGCALNVMGFGESMAGLVGLEGSKWAIRGFATAAVLLLGCINVAGVKWVIKLQFILLMILLISALDFMVGSFTSEASGGFNGWASGNFVENLWPKYDDGYSWFRVFGVFFPTVTGVLSGINMSGDLRAPSTDIPNGTLAAFGTSTFLYLVFVLFLGATCQRSFLYTDYMISVKVSAVHFLLLAGIYVSSMSSCLGAMYGTPRVLQSIAKESVIPGIDILGKGRGPNKVPLYAMAIVALVTVTFIIVGDINFLAPIVTMPFLLTYACIDYAYFALAQTFDIQEQREERFRIQASSPSYETRRYGSVSDTGNDLDLLFPDRVRHKNLQSPQNSPLHQTVPLEFDNEGPSTSNQAQSSERRTGHEADTTLAVEQAVAQAGVEQDQGDEAEPIAPIRPPIHSKTKNWYSGYCNRWASLLGAFTKLLVMLLVNWYYALTCFLVVFVVWFYVGTANPAVKPGLTAEFNFFAWLKSVIFRCFGKRMNEYEQIVVTPSCPGVDLSPTQLNEQNEDFRPRPNYHHSSVIEGRLIDDI

Target ORF information:

RefSeq Version	NM_001272202.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster uncharacterized protein, transcript variant B (CG12773), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272202.1

ORF Insert Sequence:

ATGACTAATA GCTCTGAGGG CAATGGCAAC GGCGGCCGAC GCACTGTCGA CTGGCAGAGA 
TTTGGACTTG GCGGCGACGA GGACGGGGAT GTGGCGGTGA CCAGCTTTCG CCAGCGGACC 
AACAACAACA CCGGCGGATT TGTGGATTTA GGTAACGAGT ACACGTACGC GGCTGGTGGC 
CACCATGCGT CCGGGGCCAA CGAGATCTTT GCGGGCGAGC AGGGCGACAA GCCGTGGCGC 
TCGAACTTCT TCATCTCCCA GCCCGTGCTA TTCGGCACCT GGGACGGAGT GTTCACCTCC 
TGCCTAATCA ACGTGTTTGG AGTGATCGTG TTCCTGCGAT CCGGATGGAT CGTCGCACAG 
GCAGGCATTC TGAACGCGGT GCTGATCATC TTTTGCACGG TGGTCATAGC TCTCGTCAGC 
GTCCTCTCGG CAATCGGTAT CTGCGAGCGG TGCCGTGTGG AAAGCGGTGG CGTGTACTTT 
CTCATAGCCC ACACGCTTGG TTCCCGATTC GGAGGCGCCT TGGGTTTGCT CTACTGCTTT 
GGCCAGGCGG TGGGATGCGC CCTTAACGTA ATGGGATTTG GCGAATCAAT GGCTGGACTA 
GTGGGACTCG AGGGCAGCAA GTGGGCCATC CGCGGGTTCG CTACGGCAGC GGTGCTCCTG 
TTGGGCTGCA TCAACGTGGC CGGCGTCAAG TGGGTCATCA AGCTTCAGTT TATACTGCTC 
ATGATACTGC TGATCTCGGC GCTGGACTTC ATGGTGGGCA GCTTCACTAG TGAGGCCAGT 
GGCGGCTTTA ATGGCTGGGC GAGCGGAAAC TTCGTGGAGA ACCTATGGCC AAAGTACGAC 
GACGGCTACT CCTGGTTCCG TGTGTTCGGC GTCTTCTTTC CCACCGTCAC CGGAGTGCTG 
TCGGGCATTA ACATGAGCGG CGACTTGCGC GCGCCGTCCA CGGACATTCC CAATGGCACC 
CTGGCCGCCT TTGGCACCTC CACGTTCCTG TACTTGGTGT TCGTCCTGTT TTTGGGGGCC 
ACCTGCCAAC GAAGCTTCCT GTATACTGAC TACATGATCT CTGTCAAGGT GTCTGCCGTG 
CATTTCCTGC TGTTGGCCGG CATTTATGTG TCCAGCATGT CCTCCTGCCT GGGCGCCATG 
TACGGCACTC CGCGCGTCCT TCAGAGCATC GCCAAAGAGA GCGTCATTCC GGGCATCGAT 
ATCCTGGGAA AGGGTCGTGG TCCCAACAAA GTGCCTTTAT ACGCAATGGC CATCGTGGCC 
CTAGTCACTG TCACCTTCAT TATCGTGGGA GACATCAACT TCTTGGCGCC GATCGTGACC 
ATGCCCTTCC TGCTCACATA TGCCTGTATC GACTACGCCT ACTTTGCGCT GGCCCAGACC 
TTCGACATTC AGGAGCAGCG CGAGGAGCGT TTCCGCATCC AGGCGTCGAG TCCCAGCTAC 
GAGACGCGCC GTTACGGCAG TGTTTCGGAC ACGGGCAACG ACCTGGACCT CCTGTTCCCT 
GATCGGGTGC GCCACAAGAA CCTGCAGAGT CCGCAGAACT CACCGCTGCA CCAAACAGTG 
CCCCTGGAGT TTGACAACGA GGGTCCCAGC ACCTCCAACC AGGCCCAGTC TTCGGAACGG 
AGAACGGGGC ACGAGGCGGA CACCACACTG GCCGTGGAGC AGGCAGTCGC GCAGGCCGGC 
GTCGAACAGG ACCAGGGCGA CGAGGCCGAA CCGATAGCCC CGATCCGTCC GCCCATCCAC 
TCCAAGACGA AAAACTGGTA CTCCGGCTAC TGTAATCGCT GGGCCTCTCT GCTGGGGGCC 
TTCACTAAAC TGCTGGTTAT GCTGCTAGTC AACTGGTACT ACGCCCTCAC CTGCTTCTTG 
GTGGTGTTCG TCGTATGGTT CTATGTGGGC ACAGCCAATC CGGCCGTGAA GCCGGGTCTC 
ACTGCGGAGT TTAACTTCTT CGCCTGGCTG AAGAGCGTCA TATTCCGGTG CTTCGGCAAA 
CGGATGAACG AGTACGAGCA GATAGTCGTG ACACCCTCGT GCCCAGGCGT TGATCTCTCA 
CCCACGCAGC TCAACGAGCA GAACGAGGAC TTCCGTCCGC GGCCCAACTA CCACCACTCG 
TCCGTTATCG AGGGTCGCCT CATAGATGAC ATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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CG12773 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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