Appl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Appl
Entrez Gene ID	31002
Full Name	beta amyloid protein precursor-like
Synonyms	APP,APP-like,APPL,Abeta,BcDNA:GH04413,CG7727,Dmel\CG7727,EG:65F1.5,appl,dAbeta
General protein information	Preferred Names beta amyloid protein precursor-like Names CG7727 gene product from transcript CG7727-RE APP-like Appl-PA Appl-PB Appl-PC Appl-PD Appl-PE Appl-PF CG7727-PA CG7727-PB CG7727-PC CG7727-PD CG7727-PE CG7727-PF amyloid Precursor-Like protein amyloid precursor protein amyloid precursor protein-like beta-amyloid-protein-precursor-like
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 1B9-1B10\|1-0.0 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001258519.1	NP_001245448.1	beta amyloid protein precursor-like, isoform B
NM_001258521.2	NP_001245450.1	beta amyloid protein precursor-like, isoform D
NM_001258522.2	NP_001245451.1	beta amyloid protein precursor-like, isoform E
NM_001258523.2	NP_001245452.1	beta amyloid protein precursor-like, isoform F
NM_057278.5	NP_476626.2	beta amyloid protein precursor-like, isoform A
NM_001258520.2	NP_001245449.1	beta amyloid protein precursor-like, isoform C

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Appl	NP_001245450.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002790	XP_312126.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results show that Drosophila APPL-RNAi largely mimics transgenic amyloid beta in various phenotypes which include eye degeneration, reduced longevity and motor neuron deficit functions. This is the first report showing comparable phenotypes between APPL and amyloid beta in Alzheimer's disease model of Drosophila.
Title: Knockdown of APPL mimics transgenic A? induced neurodegenerative phenotypes in Drosophila.

Appl siRNA inhibition in cortex glia resulted in longer sleep and reduced expression of genes involved in glutamate recycling.
Title: Amyloid Precursor Protein in

The results of this study showed that increased production of the endogenous Drosophila Amyloid Intracellular Domain (dAICD) caused disruption of circadian rest-activity rhythms during aging.
Title: Manipulations of amyloid precursor protein cleavage disrupt the circadian clock in aging Drosophila.

Our data demonstrate that, in addition to secreted APPL, the noncleaved form is involved in memory, raising the possibility that secreted and full-length APPL act together in memory processes.
Title: The full-length form of the Drosophila amyloid precursor protein is involved in memory formation.

memory is altered by two connected mechanisms-APPL loss-of-function and amyloid peptide toxicity-revealing in Drosophila a functional interaction between APPL and amyloid peptide.
Title: Amyloid-? Peptide Exacerbates the Memory Deficit Caused by Amyloid Precursor Protein Loss-of-Function in Drosophila.

The following Appl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Appl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa00375	NM_057278.5 Latest version!	Drosophila melanogaster beta amyloid protein precursor-like (Appl), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa03262	NM_001258522.2 Latest version!	Drosophila melanogaster beta amyloid protein precursor-like (Appl), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa03263	NM_001258521.2 Latest version!	Drosophila melanogaster beta amyloid protein precursor-like (Appl), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa03264	NM_001258523.2 Latest version!	Drosophila melanogaster beta amyloid protein precursor-like (Appl), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa03265	NM_001258520.2 Latest version!	Drosophila melanogaster beta amyloid protein precursor-like (Appl), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa03266	NM_001258519.1 Latest version!	Drosophila melanogaster beta amyloid protein precursor-like (Appl), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Appl ( NM_057278.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_476626.2 Drosophila melanogaster beta amyloid protein precursor-like (Appl), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa00375
Clone ID Related Accession (Same CDS sequence)	NM_057278.5
Accession Version	NM_057278.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	beta amyloid protein precursor-like, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_057278.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGCGCCG CGCTGCGTCG TAATTTGTTG CTGAGGAGCC TCTGGGTCGT CCTGGCCATT 
GGCACTGCTC AGGTTCAGGC CGCATCGCCG CGATGGGAGC CCCAGATTGC CGTTCTCTGT 
GAGGCCGGAC AAATCTACCA GCCGCAGTAT CTTTCCGAGG AGGGACGCTG GGTGACGGAC 
CTTAGCAAGA AGACAACCGG TCCCACATGT TTGCGTGATA AAATGGATTT GCTTGATTAC 
TGCAAGAAGG CCTATCCCAA CCGAGACATA ACCAACATTG TGGAGTCGTC CCACTACCAG 
AAGATCGGCG GCTGGTGTCG CCAGGGGGCA TTGAATGCGG CTAAGTGCAA GGGATCCCAC 
CGATGGATCA AGCCATTCCG CTGTCTCGGA CCATTCCAAT CTGACGCCCT GCTAGTTCCC 
GAGGGCTGTC TGTTCGACCA CATCCACAAC GCCTCCCGAT GCTGGCCATT TGTGAGGTGG 
AACCAAACCG GAGCAGCCGC TTGCCAGGAG CGCGGAATGC AGATGCGCAG CTTCGCCATG 
CTTCTGCCCT GCGGAATCTC GGTCTTCTCC GGAGTGGAGT TCGTCTGCTG TCCCAAGCAC 
TTCAAAACCG ATGAAATTCA TGTAAAGAAG ACCGACTTGC CGGTGATGCC GGCAGCTCAG 
ATCAATAGCG CCAATGACGA GCTGGTGATG AATGACGAAG ACGACAGCAA CGACTCGAAC 
TACTCGAAGG ATGCCAATGA AGACGATCTG GACGATGAGG ATGATTTGAT GGGTGATGAT 
GAGGAGGACG ATATGGTGGC GGATGAGGCA GCTACCGCAG GCGGAAGCCC CAACACCGGT 
TCCTCAGGTG ACTCAAATAG CGGATCTTTG GATGACATAA ATGCGGAGTA CGATAGCGGA 
GAGGAGGGTG ACAACTACGA GGAGGACGGT GCTGGTTCCG AAAGCGAGGC TGAGGTTGAG 
GCCTCCTGGG ATCAGAGCGG AGGGGCGAAG GTTGTGTCCC TAAAAAGCGA TTCCTCATCC 
CCGTCTAGCG CTCCGGTTGC TCCCGCCCCC GAGAAGGCAC CTGTGAAGTC CGAATCGGTC 
ACGAGCACTC CTCAGCTGTC TGCCTCTGCC GCTGCTTTTG TAGCTGCCAA TTCTGGAAAC 
TCGGGAACCG GAGCCGGAGC ACCACCATCC ACTGCACAAC CCACATCGGA TCCCTACTTC 
ACCCATTTTG ATCCGCACTA CGAGCACCAG AGCTACAAAG TAAGTCAGAA ACGCCTTGAG 
GAATCGCACC GCGAGAAGGT CACGCGCGTT ATGAAGGACT GGTCCGATCT CGAGGAGAAG 
TACCAGGACA TGCGCCTAGC GGATCCCAAG GCAGCCCAGT CATTTAAGCA GCGGATGACG 
GCTCGCTTCC AGACTTCTGT TCAGGCACTC GAGGAAGAAG GCAACGCCGA GAAGCACCAG 
CTGGCCGCCA TGCACCAGCA GCGCGTTTTG GCCCACATCA ACCAGCGGAA GCGCGAGGCC 
ATGACCTGCT ACACCCAGGC CCTGACTGAG CAGCCCCCGA ATGCCCACCA CGTTGAGAAG 
TGTCTACAGA AGCTGCTGCG CGCCCTGCAC AAGGACCGGG CCCACGCCTT GGCCCACTAC 
CGGCACCTAT TGAACTCTGG AGGACCTGGT GGACTAGAAG CAGCTGCTTC GGAGCGTCCA 
CGCACGCTGG AGCGCCTAAT CGACATCGAT AGAGCCGTAA ACCAGTCGAT GACCATGCTC 
AAGCGCTATC CGGAGCTCTC CGCCAAGATT GCCCAGCTGA TGAATGACTA CATTCTGGCA 
CTGCGCAGCA AGGATGACAT TCCCGGCTCC TCGCTGGGAA TGAGCGAGGA GGCGGAGGCC 
GGCATTCTGG ACAAGTACCG CGTCGAGATC GAGCGCAAGG TGGCCGAGAA AGAGCGACTA 
CGGCTGGCGG AGAAGCAGCG CAAGGAGCAG CGTGCCGCTG AGCGGGAAAA ACTGCGCGAG 
GAGAAGCTGC GCCTGGAGGC CAAAAAGGTG GACGACATGC TCAAGTCACA GGTGGCCGAG 
CAGCAGTCCC AGCCGACCCA GTCGTCAACC CAATCGCAGG CGCAGCAGCA GCAGCAGGAA 
AAGAGCCTTC CCGGCAAGGA GCTGGGTCCC GACGCAGCTC TGGTCACAGC GGCCAATCCC 
AACCTAGAGA CCACCAAAAG CGAGAAGGAT CTGTCGGACA CTGAGTATGG GGAGGCAACA 
GTGAGCAGCA CCAAGGTGCA GACCGTGCTG CCAACGGTCG ACGACGATGC CGTCCAGCGG 
GCAGTGGAGG ACGTAGCCGC CGCCGTCGCC CACCAGGAGG CCGAGCCGCA GGTGCAGCAC 
TTCATGACCC ACGACCTGGG CCACCGCGAA TCGAGCTTCT CGCTGCGCCG CGAGTTCGCG 
CAACATGCAC ACGCGGCCAA AGAGGGCCGC AACGTCTACT TCACGCTCTC CTTCGCCGGG 
ATCGCCCTGA TGGCGGCCGT TTTCGTTGGC GTGGCCGTGG CCAAATGGCG AACATCGCGC 
TCGCCGCACG CCCAGGGCTT CATCGAGGTC GACCAGAACG TCACAACACA CCATCCCATT 
GTGCGCGAGG AGAAGATCGT GCCCAACATG CAGATCAATG GGTACGAGAA TCCGACCTAC 
AAGTATTTCG AAGTGAAAGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476626.2
CDS	207..2870
Translation	MCAALRRNLLLRSLWVVLAIGTAQVQAASPRWEPQIAVLCEAGQIYQPQYLSEEGRWVTDLSKKTTGPTCLRDKMDLLDYCKKAYPNRDITNIVESSHYQKIGGWCRQGALNAAKCKGSHRWIKPFRCLGPFQSDALLVPEGCLFDHIHNASRCWPFVRWNQTGAAACQERGMQMRSFAMLLPCGISVFSGVEFVCCPKHFKTDEIHVKKTDLPVMPAAQINSANDELVMNDEDDSNDSNYSKDANEDDLDDEDDLMGDDEEDDMVADEAATAGGSPNTGSSGDSNSGSLDDINAEYDSGEEGDNYEEDGAGSESEAEVEASWDQSGGAKVVSLKSDSSSPSSAPVAPAPEKAPVKSESVTSTPQLSASAAAFVAANSGNSGTGAGAPPSTAQPTSDPYFTHFDPHYEHQSYKVSQKRLEESHREKVTRVMKDWSDLEEKYQDMRLADPKAAQSFKQRMTARFQTSVQALEEEGNAEKHQLAAMHQQRVLAHINQRKREAMTCYTQALTEQPPNAHHVEKCLQKLLRALHKDRAHALAHYRHLLNSGGPGGLEAAASERPRTLERLIDIDRAVNQSMTMLKRYPELSAKIAQLMNDYILALRSKDDIPGSSLGMSEEAEAGILDKYRVEIERKVAEKERLRLAEKQRKEQRAAEREKLREEKLRLEAKKVDDMLKSQVAEQQSQPTQSSTQSQAQQQQQEKSLPGKELGPDAALVTAANPNLETTKSEKDLSDTEYGEATVSSTKVQTVLPTVDDDAVQRAVEDVAAAVAHQEAEPQVQHFMTHDLGHRESSFSLRREFAQHAHAAKEGRNVYFTLSFAGIALMAAVFVGVAVAKWRTSRSPHAQGFIEVDQNVTTHHPIVREEKIVPNMQINGYENPTYKYFEVKE

Target ORF information:

RefSeq Version	NM_057278.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster beta amyloid protein precursor-like (Appl), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057278.5

ORF Insert Sequence:

ATGTGCGCCG CGCTGCGTCG TAATTTGTTG CTGAGGAGCC TCTGGGTCGT CCTGGCCATT 
GGCACTGCTC AGGTTCAGGC CGCATCGCCG CGATGGGAGC CCCAGATTGC CGTTCTCTGT 
GAGGCCGGAC AAATCTACCA GCCGCAGTAT CTTTCCGAGG AGGGACGCTG GGTGACGGAC 
CTTAGCAAGA AGACAACCGG TCCCACATGT TTGCGTGATA AAATGGATTT GCTTGATTAC 
TGCAAGAAGG CCTATCCCAA CCGAGACATA ACCAACATTG TGGAGTCGTC CCACTACCAG 
AAGATCGGCG GCTGGTGTCG CCAGGGGGCA TTGAATGCGG CTAAGTGCAA GGGATCCCAC 
CGATGGATCA AGCCATTCCG CTGTCTCGGA CCATTCCAAT CTGACGCCCT GCTAGTTCCC 
GAGGGCTGTC TGTTCGACCA CATCCACAAC GCCTCCCGAT GCTGGCCATT TGTGAGGTGG 
AACCAAACCG GAGCAGCCGC TTGCCAGGAG CGCGGAATGC AGATGCGCAG CTTCGCCATG 
CTTCTGCCCT GCGGAATCTC GGTCTTCTCC GGAGTGGAGT TCGTCTGCTG TCCCAAGCAC 
TTCAAAACCG ATGAAATTCA TGTAAAGAAG ACCGACTTGC CGGTGATGCC GGCAGCTCAG 
ATCAATAGCG CCAATGACGA GCTGGTGATG AATGACGAAG ACGACAGCAA CGACTCGAAC 
TACTCGAAGG ATGCCAATGA AGACGATCTG GACGATGAGG ATGATTTGAT GGGTGATGAT 
GAGGAGGACG ATATGGTGGC GGATGAGGCA GCTACCGCAG GCGGAAGCCC CAACACCGGT 
TCCTCAGGTG ACTCAAATAG CGGATCTTTG GATGACATAA ATGCGGAGTA CGATAGCGGA 
GAGGAGGGTG ACAACTACGA GGAGGACGGT GCTGGTTCCG AAAGCGAGGC TGAGGTTGAG 
GCCTCCTGGG ATCAGAGCGG AGGGGCGAAG GTTGTGTCCC TAAAAAGCGA TTCCTCATCC 
CCGTCTAGCG CTCCGGTTGC TCCCGCCCCC GAGAAGGCAC CTGTGAAGTC CGAATCGGTC 
ACGAGCACTC CTCAGCTGTC TGCCTCTGCC GCTGCTTTTG TAGCTGCCAA TTCTGGAAAC 
TCGGGAACCG GAGCCGGAGC ACCACCATCC ACTGCACAAC CCACATCGGA TCCCTACTTC 
ACCCATTTTG ATCCGCACTA CGAGCACCAG AGCTACAAAG TAAGTCAGAA ACGCCTTGAG 
GAATCGCACC GCGAGAAGGT CACGCGCGTT ATGAAGGACT GGTCCGATCT CGAGGAGAAG 
TACCAGGACA TGCGCCTAGC GGATCCCAAG GCAGCCCAGT CATTTAAGCA GCGGATGACG 
GCTCGCTTCC AGACTTCTGT TCAGGCACTC GAGGAAGAAG GCAACGCCGA GAAGCACCAG 
CTGGCCGCCA TGCACCAGCA GCGCGTTTTG GCCCACATCA ACCAGCGGAA GCGCGAGGCC 
ATGACCTGCT ACACCCAGGC CCTGACTGAG CAGCCCCCGA ATGCCCACCA CGTTGAGAAG 
TGTCTACAGA AGCTGCTGCG CGCCCTGCAC AAGGACCGGG CCCACGCCTT GGCCCACTAC 
CGGCACCTAT TGAACTCTGG AGGACCTGGT GGACTAGAAG CAGCTGCTTC GGAGCGTCCA 
CGCACGCTGG AGCGCCTAAT CGACATCGAT AGAGCCGTAA ACCAGTCGAT GACCATGCTC 
AAGCGCTATC CGGAGCTCTC CGCCAAGATT GCCCAGCTGA TGAATGACTA CATTCTGGCA 
CTGCGCAGCA AGGATGACAT TCCCGGCTCC TCGCTGGGAA TGAGCGAGGA GGCGGAGGCC 
GGCATTCTGG ACAAGTACCG CGTCGAGATC GAGCGCAAGG TGGCCGAGAA AGAGCGACTA 
CGGCTGGCGG AGAAGCAGCG CAAGGAGCAG CGTGCCGCTG AGCGGGAAAA ACTGCGCGAG 
GAGAAGCTGC GCCTGGAGGC CAAAAAGGTG GACGACATGC TCAAGTCACA GGTGGCCGAG 
CAGCAGTCCC AGCCGACCCA GTCGTCAACC CAATCGCAGG CGCAGCAGCA GCAGCAGGAA 
AAGAGCCTTC CCGGCAAGGA GCTGGGTCCC GACGCAGCTC TGGTCACAGC GGCCAATCCC 
AACCTAGAGA CCACCAAAAG CGAGAAGGAT CTGTCGGACA CTGAGTATGG GGAGGCAACA 
GTGAGCAGCA CCAAGGTGCA GACCGTGCTG CCAACGGTCG ACGACGATGC CGTCCAGCGG 
GCAGTGGAGG ACGTAGCCGC CGCCGTCGCC CACCAGGAGG CCGAGCCGCA GGTGCAGCAC 
TTCATGACCC ACGACCTGGG CCACCGCGAA TCGAGCTTCT CGCTGCGCCG CGAGTTCGCG 
CAACATGCAC ACGCGGCCAA AGAGGGCCGC AACGTCTACT TCACGCTCTC CTTCGCCGGG 
ATCGCCCTGA TGGCGGCCGT TTTCGTTGGC GTGGCCGTGG CCAAATGGCG AACATCGCGC 
TCGCCGCACG CCCAGGGCTT CATCGAGGTC GACCAGAACG TCACAACACA CCATCCCATT 
GTGCGCGAGG AGAAGATCGT GCCCAACATG CAGATCAATG GGTACGAGAA TCCGACCTAC 
AAGTATTTCG AAGTGAAAGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Appl ( NM_001258522.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245451.1 Drosophila melanogaster beta amyloid protein precursor-like (Appl), transcript variant E, mRNA.

Price & Availability↑

CloneID	OFa03262
Clone ID Related Accession (Same CDS sequence)	NM_001258522.2
Accession Version	NM_001258522.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	beta amyloid protein precursor-like, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001258522.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGCGCCG CGCTGCGTCG TAATTTGTTG CTGAGGAGCC TCTGGGTCGT CCTGGCCATT 
GGCACTGCTC AGGTTCAGGC CGCATCGCCG CGATGGGAGC CCCAGATTGC CGTTCTCTGT 
GAGGCCGGAC AAATCTACCA GCCGCAGTAT CTTTCCGAGG AGGGACGCTG GGTGACGGAC 
CTTAGCAAGA AGACAACCGG TCCCACATGT TTGCGTGATA AAATGGATTT GCTTGATTAC 
TGCAAGAAGG CCTATCCCAA CCGAGACATA ACCAACATTG TGGAGTCGTC CCACTACCAG 
AAGATCGGCG GCTGGTGTCG CCAGGGGGCA TTGAATGCGG CTAAGTGCAA GGGATCCCAC 
CGATGGATCA AGCCATTCCG CTGTCTCGGA CCATTCCAAT CTGACGCCCT GCTAGTTCCC 
GAGGGCTGTC TGTTCGACCA CATCCACAAC GCCTCCCGAT GCTGGCCATT TGTGAGGTGG 
AACCAAACCG GAGCAGCCGC TTGCCAGGAG CGCGGAATGC AGATGCGCAG CTTCGCCATG 
CTTCTGCCCT GCGGAATCTC GGTCTTCTCC GGAGTGGAGT TCGTCTGCTG TCCCAAGCAC 
TTCAAAACCG ATGAAATTCA TGTAAAGAAG ACCGACTTGC CGGTGATGCC GGCAGCTCAG 
ATCAATAGCG CCAATGACGA GCTGGTGATG AATGACGAAG ACGACAGCAA CGACTCGAAC 
TACTCGAAGG ATGCCAATGA AGACGATCTG GACGATGAGG ATGATTTGAT GGGTGATGAT 
GAGGAGGACG ATATGGTGGC GGATGAGGCA GCTACCGCAG GCGGAAGCCC CAACACCGGT 
TCCTCAGGTG ACTCAAATAG CGGATCTTTG GATGACATAA ATGCGGAGTA CGATAGCGGA 
GAGGAGGGTG ACAACTACGA GGAGGACGGT GCTGGTTCCG AAAGCGAGGC TGAGGTTGAG 
GCCTCCTGGG ATCAGAGCGG AGGGGCGAAG GTTGTGTCCC TAAAAAGCGA TTCCTCATCC 
CCGTCTAGCG CTCCGGTTGC TCCCGCCCCC GAGAAGGCAC CTGTGAAGTC CGAATCGGTC 
ACGAGCACTC CTCAGCTGTC TGCCTCTGCC GCTGCTTTTG TAGCTGCCAA TTCTGGAAAC 
TCGGGAACCG GAGCCGGAGC ACCACCATCC ACTGCACAAC CCACATCGGA TCCCTACTTC 
ACCCATTTTG ATCCGCACTA CGAGCACCAG AGCTACAAAC GCCTTGAGGA ATCGCACCGC 
GAGAAGGTCA CGCGCGTTAT GAAGGACTGG TCCGATCTCG AGGAGAAGTA CCAGGACATG 
CGCCTAGCGG ATCCCAAGGC AGCCCAGTCA TTTAAGCAGC GGATGACGGC TCGCTTCCAG 
ACTTCTGTTC AGGCACTCGA GGAAGAAGGC AACGCCGAGA AGCACCAGCT GGCCGCCATG 
CACCAGCAGC GCGTTTTGGC CCACATCAAC CAGCGGAAGC GCGAGGCCAT GACCTGCTAC 
ACCCAGGCCC TGACTGAGCA GCCCCCGAAT GCCCACCACG TTGAGAAGTG TCTACAGAAG 
CTGCTGCGCG CCCTGCACAA GGACCGGGCC CACGCCTTGG CCCACTACCG GCACCTATTG 
AACTCTGGAG GACCTGGTGG ACTAGAAGCA GCTGCTTCGG AGCGTCCACG CACGCTGGAG 
CGCCTAATCG ACATCGATAG AGCCGTAAAC CAGTCGATGA CCATGCTCAA GCGCTATCCG 
GAGCTCTCCG CCAAGATTGC CCAGCTGATG AATGACTACA TTCTGGCACT GCGCAGCAAG 
GATGACATTC CCGGCTCCTC GCTGGGAATG AGCGAGGAGG CGGAGGCCGG CATTCTGGAC 
AAGTACCGCG TCGAGATCGA GCGCAAGGTG GCCGAGAAAG AGCGACTACG GCTGGCGGAG 
AAGCAGCGCA AGGAGCAGCG TGCCGCTGAG CGGGAAAAAC TGCGCGAGGA GAAGCTGCGC 
CTGGAGGCCA AAAAGGTGGA CGACATGCTC AAGTCACAGG TGGCCGAGCA GCAGTCCCAG 
CCGACCCAGT CGTCAACCCA ATCGCAGGCG CAGCAGCAGC AGCAGGAAAA GAGCCTTCCC 
GGCAAGGAGC TGGGTCCCGA CGCAGCTCTG GTCACAGCGG CCAATCCCAA CCTAGAGACC 
ACCAAAAGCG AGAAGGATCT GTCGGACACT GAGTATGGGG AGGCAACAGT GAGCAGCACC 
AAGGTGCAGA CCGTGCTGCC AACGGTCGAC GACGATGCCG TCCAGCGGGC AGTGGAGGAC 
GTAGCCGCCG CCGTCGCCCA CCAGGAGGCC GAGCCGCAGG TGCAGCACTT CATGACCCAC 
GACCTGGGCC ACCGCGAATC GAGCTTCTCG CTGCGCCGCG AGTTCGCGCA ACATGCACAC 
GCGGCCAAAG AGGGCCGCAA CGTCTACTTC ACGCTCTCCT TCGCCGGGAT CGCCCTGATG 
GCGGCCGTTT TCGTTGGCGT GGCCGTGGCC AAATGGCGAA CATCGCGCTC GCCGCACGCC 
CAGGGCTTCA TCGAGGTCGA CCAGAACGTC ACAACACACC ATCCCATTGT GCGCGAGGAG 
AAGATCGTGC CCAACATGCA GATCAATGGG TACGAGAATC CGACCTACAA GTATTTCGAA 
GTGAAAGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245451.1
CDS	207..2858
Translation	MCAALRRNLLLRSLWVVLAIGTAQVQAASPRWEPQIAVLCEAGQIYQPQYLSEEGRWVTDLSKKTTGPTCLRDKMDLLDYCKKAYPNRDITNIVESSHYQKIGGWCRQGALNAAKCKGSHRWIKPFRCLGPFQSDALLVPEGCLFDHIHNASRCWPFVRWNQTGAAACQERGMQMRSFAMLLPCGISVFSGVEFVCCPKHFKTDEIHVKKTDLPVMPAAQINSANDELVMNDEDDSNDSNYSKDANEDDLDDEDDLMGDDEEDDMVADEAATAGGSPNTGSSGDSNSGSLDDINAEYDSGEEGDNYEEDGAGSESEAEVEASWDQSGGAKVVSLKSDSSSPSSAPVAPAPEKAPVKSESVTSTPQLSASAAAFVAANSGNSGTGAGAPPSTAQPTSDPYFTHFDPHYEHQSYKRLEESHREKVTRVMKDWSDLEEKYQDMRLADPKAAQSFKQRMTARFQTSVQALEEEGNAEKHQLAAMHQQRVLAHINQRKREAMTCYTQALTEQPPNAHHVEKCLQKLLRALHKDRAHALAHYRHLLNSGGPGGLEAAASERPRTLERLIDIDRAVNQSMTMLKRYPELSAKIAQLMNDYILALRSKDDIPGSSLGMSEEAEAGILDKYRVEIERKVAEKERLRLAEKQRKEQRAAEREKLREEKLRLEAKKVDDMLKSQVAEQQSQPTQSSTQSQAQQQQQEKSLPGKELGPDAALVTAANPNLETTKSEKDLSDTEYGEATVSSTKVQTVLPTVDDDAVQRAVEDVAAAVAHQEAEPQVQHFMTHDLGHRESSFSLRREFAQHAHAAKEGRNVYFTLSFAGIALMAAVFVGVAVAKWRTSRSPHAQGFIEVDQNVTTHHPIVREEKIVPNMQINGYENPTYKYFEVKE

Target ORF information:

RefSeq Version	NM_001258522.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster beta amyloid protein precursor-like (Appl), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258522.2

ORF Insert Sequence:

ATGTGCGCCG CGCTGCGTCG TAATTTGTTG CTGAGGAGCC TCTGGGTCGT CCTGGCCATT 
GGCACTGCTC AGGTTCAGGC CGCATCGCCG CGATGGGAGC CCCAGATTGC CGTTCTCTGT 
GAGGCCGGAC AAATCTACCA GCCGCAGTAT CTTTCCGAGG AGGGACGCTG GGTGACGGAC 
CTTAGCAAGA AGACAACCGG TCCCACATGT TTGCGTGATA AAATGGATTT GCTTGATTAC 
TGCAAGAAGG CCTATCCCAA CCGAGACATA ACCAACATTG TGGAGTCGTC CCACTACCAG 
AAGATCGGCG GCTGGTGTCG CCAGGGGGCA TTGAATGCGG CTAAGTGCAA GGGATCCCAC 
CGATGGATCA AGCCATTCCG CTGTCTCGGA CCATTCCAAT CTGACGCCCT GCTAGTTCCC 
GAGGGCTGTC TGTTCGACCA CATCCACAAC GCCTCCCGAT GCTGGCCATT TGTGAGGTGG 
AACCAAACCG GAGCAGCCGC TTGCCAGGAG CGCGGAATGC AGATGCGCAG CTTCGCCATG 
CTTCTGCCCT GCGGAATCTC GGTCTTCTCC GGAGTGGAGT TCGTCTGCTG TCCCAAGCAC 
TTCAAAACCG ATGAAATTCA TGTAAAGAAG ACCGACTTGC CGGTGATGCC GGCAGCTCAG 
ATCAATAGCG CCAATGACGA GCTGGTGATG AATGACGAAG ACGACAGCAA CGACTCGAAC 
TACTCGAAGG ATGCCAATGA AGACGATCTG GACGATGAGG ATGATTTGAT GGGTGATGAT 
GAGGAGGACG ATATGGTGGC GGATGAGGCA GCTACCGCAG GCGGAAGCCC CAACACCGGT 
TCCTCAGGTG ACTCAAATAG CGGATCTTTG GATGACATAA ATGCGGAGTA CGATAGCGGA 
GAGGAGGGTG ACAACTACGA GGAGGACGGT GCTGGTTCCG AAAGCGAGGC TGAGGTTGAG 
GCCTCCTGGG ATCAGAGCGG AGGGGCGAAG GTTGTGTCCC TAAAAAGCGA TTCCTCATCC 
CCGTCTAGCG CTCCGGTTGC TCCCGCCCCC GAGAAGGCAC CTGTGAAGTC CGAATCGGTC 
ACGAGCACTC CTCAGCTGTC TGCCTCTGCC GCTGCTTTTG TAGCTGCCAA TTCTGGAAAC 
TCGGGAACCG GAGCCGGAGC ACCACCATCC ACTGCACAAC CCACATCGGA TCCCTACTTC 
ACCCATTTTG ATCCGCACTA CGAGCACCAG AGCTACAAAC GCCTTGAGGA ATCGCACCGC 
GAGAAGGTCA CGCGCGTTAT GAAGGACTGG TCCGATCTCG AGGAGAAGTA CCAGGACATG 
CGCCTAGCGG ATCCCAAGGC AGCCCAGTCA TTTAAGCAGC GGATGACGGC TCGCTTCCAG 
ACTTCTGTTC AGGCACTCGA GGAAGAAGGC AACGCCGAGA AGCACCAGCT GGCCGCCATG 
CACCAGCAGC GCGTTTTGGC CCACATCAAC CAGCGGAAGC GCGAGGCCAT GACCTGCTAC 
ACCCAGGCCC TGACTGAGCA GCCCCCGAAT GCCCACCACG TTGAGAAGTG TCTACAGAAG 
CTGCTGCGCG CCCTGCACAA GGACCGGGCC CACGCCTTGG CCCACTACCG GCACCTATTG 
AACTCTGGAG GACCTGGTGG ACTAGAAGCA GCTGCTTCGG AGCGTCCACG CACGCTGGAG 
CGCCTAATCG ACATCGATAG AGCCGTAAAC CAGTCGATGA CCATGCTCAA GCGCTATCCG 
GAGCTCTCCG CCAAGATTGC CCAGCTGATG AATGACTACA TTCTGGCACT GCGCAGCAAG 
GATGACATTC CCGGCTCCTC GCTGGGAATG AGCGAGGAGG CGGAGGCCGG CATTCTGGAC 
AAGTACCGCG TCGAGATCGA GCGCAAGGTG GCCGAGAAAG AGCGACTACG GCTGGCGGAG 
AAGCAGCGCA AGGAGCAGCG TGCCGCTGAG CGGGAAAAAC TGCGCGAGGA GAAGCTGCGC 
CTGGAGGCCA AAAAGGTGGA CGACATGCTC AAGTCACAGG TGGCCGAGCA GCAGTCCCAG 
CCGACCCAGT CGTCAACCCA ATCGCAGGCG CAGCAGCAGC AGCAGGAAAA GAGCCTTCCC 
GGCAAGGAGC TGGGTCCCGA CGCAGCTCTG GTCACAGCGG CCAATCCCAA CCTAGAGACC 
ACCAAAAGCG AGAAGGATCT GTCGGACACT GAGTATGGGG AGGCAACAGT GAGCAGCACC 
AAGGTGCAGA CCGTGCTGCC AACGGTCGAC GACGATGCCG TCCAGCGGGC AGTGGAGGAC 
GTAGCCGCCG CCGTCGCCCA CCAGGAGGCC GAGCCGCAGG TGCAGCACTT CATGACCCAC 
GACCTGGGCC ACCGCGAATC GAGCTTCTCG CTGCGCCGCG AGTTCGCGCA ACATGCACAC 
GCGGCCAAAG AGGGCCGCAA CGTCTACTTC ACGCTCTCCT TCGCCGGGAT CGCCCTGATG 
GCGGCCGTTT TCGTTGGCGT GGCCGTGGCC AAATGGCGAA CATCGCGCTC GCCGCACGCC 
CAGGGCTTCA TCGAGGTCGA CCAGAACGTC ACAACACACC ATCCCATTGT GCGCGAGGAG 
AAGATCGTGC CCAACATGCA GATCAATGGG TACGAGAATC CGACCTACAA GTATTTCGAA 
GTGAAAGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Appl ( NM_001258521.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245450.1 Drosophila melanogaster beta amyloid protein precursor-like (Appl), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa03263
Clone ID Related Accession (Same CDS sequence)	NM_001258521.2
Accession Version	NM_001258521.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	beta amyloid protein precursor-like, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001258521.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGCGCCG CGCTGCGTCG TAATTTGTTG CTGAGGAGCC TCTGGGTCGT CCTGGCCATT 
GGCACTGCTC AGGTTCAGGC CGCATCGCCG CGATGGGAGC CCCAGATTGC CGTTCTCTGT 
GAGGCCGGAC AAATCTACCA GCCGCAGTAT CTTTCCGAGG AGGGACGCTG GGTGACGGAC 
CTTAGCAAGA AGACAACCGG TCCCACATGT TTGCGTGATA AAATGGATTT GCTTGATTAC 
TGCAAGAAGG CCTATCCCAA CCGAGACATA ACCAACATTG TGGAGTCGTC CCACTACCAG 
AAGATCGGCG GCTGGTGTCG CCAGGGGGCA TTGAATGCGG CTAAGTGCAA GGGATCCCAC 
CGATGGATCA AGCCATTCCG CTGTCTCGAA GGACCATTCC AATCTGACGC CCTGCTAGTT 
CCCGAGGGCT GTCTGTTCGA CCACATCCAC AACGCCTCCC GATGCTGGCC ATTTGTGAGG 
TGGAACCAAA CCGGAGCAGC CGCTTGCCAG GAGCGCGGAA TGCAGATGCG CAGCTTCGCC 
ATGCTTCTGC CCTGCGGAAT CTCGGTCTTC TCCGGAGTGG AGTTCGTCTG CTGTCCCAAG 
CACTTCAAAA CCGATGAAAT TCATGTAAAG AAGACCGACT TGCCGGTGAT GCCGGCAGCT 
CAGATCAATA GCGCCAATGA CGAGCTGGTG ATGAATGACG AAGACGACAG CAACGACTCG 
AACTACTCGA AGGATGCCAA TGAAGACGAT CTGGACGATG AGGATGATTT GATGGGTGAT 
GATGAGGAGG ACGATATGGT GGCGGATGAG GCAGCTACCG CAGGCGGAAG CCCCAACACC 
GGTTCCTCAG GTGACTCAAA TAGCGGATCT TTGGATGACA TAAATGCGGA GTACGATAGC 
GGAGAGGAGG GTGACAACTA CGAGGAGGAC GGTGCTGGTT CCGAAAGCGA GGCTGAGGTT 
GAGGCCTCCT GGGATCAGAG CGGAGGGGCG AAGGTTGTGT CCCTAAAAAG CGATTCCTCA 
TCCCCGTCTA GCGCTCCGGT TGCTCCCGCC CCCGAGAAGG CACCTGTGAA GTCCGAATCG 
GTCACGAGCA CTCCTCAGCT GTCTGCCTCT GCCGCTGCTT TTGTAGCTGC CAATTCTGGA 
AACTCGGGAA CCGGAGCCGG AGCACCACCA TCCACTGCAC AACCCACATC GGATCCCTAC 
TTCACCCATT TTGATCCGCA CTACGAGCAC CAGAGCTACA AAGTAAGTCA GAAAGAAGCC 
CAACAGCGCC TTGAGGAATC GCACCGCGAG AAGGTCACGC GCGTTATGAA GGACTGGTCC 
GATCTCGAGG AGAAGTACCA GGACATGCGC CTAGCGGATC CCAAGGCAGC CCAGTCATTT 
AAGCAGCGGA TGACGGCTCG CTTCCAGGCA CTCGAGGAAG AAGGCAACGC CGAGAAGCAC 
CAGCTGGCCG CCATGCACCA GCAGCGCGTT TTGGCCCACA TCAACCAGCG GAAGCGCGAG 
GCCATGACCT GCTACACCCA GGCCCTGACT GAGCAGCCCC CGAATGCCCA CCACGTTGAG 
AAGTGTCTAC AGAAGCTGCT GCGCGCCCTG CACAAGGACC GGGCCCACGC CTTGGCCCAC 
TACCGGCACC TATTGAACTC TGGAGGACCT GGTGGACTAG AAGCAGCTGC TTCGGAGCGT 
CCACGCACGC TGGAGCGCCT AATCGACATC GATAGAGCCG TAAACCAGTC GATGACCATG 
CTCAAGCGCT ATCCGGAGCT CTCCGCCAAG ATTGCCCAGC TGATGAATGA CTACATTCTG 
GCACTGCGCA GCAAGGATGA CATTCCCGGC TCCTCGCTGG GAATGAGCGA GGAGGCGGAG 
GCCGGCATTC TGGACAAGTA CCGCGTCGAG ATCGAGCGCA AGGTGGCCGA GAAAGAGCGA 
CTACGGCTGG CGGAGAAGCA GCGCAAGGAG CAGCGTGCCG CTGAGCGGGA AAAACTGCGC 
GAGGAGAAGC TGCGCCTGGA GGCCAAAAAG GTGGACGACA TGCTCAAGTC ACAGGTGGCC 
GAGCAGCAGT CCCAGCCGAC CCAGTCGTCA ACCCAATCGC AGGCGCAGCA GCAGCAGCAG 
GAAAAGAGCC TTCCCGGCAA GGAGCTGGGT CCCGACGCAG CTCTGGTCAC AGCGGCCAAT 
CCCAACCTAG AGACCACCAA AAGCGAGAAG GATCTGTCGG ACACTGAGTA TGGGGAGGCA 
ACAGTGAGCA GCACCAAGGT GCAGACCGTG CTGCCAACGG TCGACGACGA TGCCGTCCAG 
CGGGCAGTGG AGGACGTAGC CGCCGCCGTC GCCCACCAGG AGGCCGAGCC GCAGGTGCAG 
CACTTCATGA CCCACGACCT GGGCCACCGC GAATCGAGCT TCTCGCTGCG CCGCGAGTTC 
GCGCAACATG CACACGCGGC CAAAGAGGGC CGCAACGTCT ACTTCACGCT CTCCTTCGCC 
GGGATCGCCC TGATGGCGGC CGTTTTCGTT GGCGTGGCCG TGGCCAAATG GCGAACATCG 
CGCTCGCCGC ACGCCCAGGG CTTCATCGAG GTCGACCAGA ACGTCACAAC ACACCATCCC 
ATTGTGCGCG AGGAGAAGAT CGTGCCCAAC ATGCAGATCA ATGGGTACGA GAATCCGACC 
TACAAGTATT TCGAAGTGAA AGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245450.1
CDS	207..2873
Translation	MCAALRRNLLLRSLWVVLAIGTAQVQAASPRWEPQIAVLCEAGQIYQPQYLSEEGRWVTDLSKKTTGPTCLRDKMDLLDYCKKAYPNRDITNIVESSHYQKIGGWCRQGALNAAKCKGSHRWIKPFRCLEGPFQSDALLVPEGCLFDHIHNASRCWPFVRWNQTGAAACQERGMQMRSFAMLLPCGISVFSGVEFVCCPKHFKTDEIHVKKTDLPVMPAAQINSANDELVMNDEDDSNDSNYSKDANEDDLDDEDDLMGDDEEDDMVADEAATAGGSPNTGSSGDSNSGSLDDINAEYDSGEEGDNYEEDGAGSESEAEVEASWDQSGGAKVVSLKSDSSSPSSAPVAPAPEKAPVKSESVTSTPQLSASAAAFVAANSGNSGTGAGAPPSTAQPTSDPYFTHFDPHYEHQSYKVSQKEAQQRLEESHREKVTRVMKDWSDLEEKYQDMRLADPKAAQSFKQRMTARFQALEEEGNAEKHQLAAMHQQRVLAHINQRKREAMTCYTQALTEQPPNAHHVEKCLQKLLRALHKDRAHALAHYRHLLNSGGPGGLEAAASERPRTLERLIDIDRAVNQSMTMLKRYPELSAKIAQLMNDYILALRSKDDIPGSSLGMSEEAEAGILDKYRVEIERKVAEKERLRLAEKQRKEQRAAEREKLREEKLRLEAKKVDDMLKSQVAEQQSQPTQSSTQSQAQQQQQEKSLPGKELGPDAALVTAANPNLETTKSEKDLSDTEYGEATVSSTKVQTVLPTVDDDAVQRAVEDVAAAVAHQEAEPQVQHFMTHDLGHRESSFSLRREFAQHAHAAKEGRNVYFTLSFAGIALMAAVFVGVAVAKWRTSRSPHAQGFIEVDQNVTTHHPIVREEKIVPNMQINGYENPTYKYFEVKE

Target ORF information:

RefSeq Version	NM_001258521.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster beta amyloid protein precursor-like (Appl), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258521.2

ORF Insert Sequence:

ATGTGCGCCG CGCTGCGTCG TAATTTGTTG CTGAGGAGCC TCTGGGTCGT CCTGGCCATT 
GGCACTGCTC AGGTTCAGGC CGCATCGCCG CGATGGGAGC CCCAGATTGC CGTTCTCTGT 
GAGGCCGGAC AAATCTACCA GCCGCAGTAT CTTTCCGAGG AGGGACGCTG GGTGACGGAC 
CTTAGCAAGA AGACAACCGG TCCCACATGT TTGCGTGATA AAATGGATTT GCTTGATTAC 
TGCAAGAAGG CCTATCCCAA CCGAGACATA ACCAACATTG TGGAGTCGTC CCACTACCAG 
AAGATCGGCG GCTGGTGTCG CCAGGGGGCA TTGAATGCGG CTAAGTGCAA GGGATCCCAC 
CGATGGATCA AGCCATTCCG CTGTCTCGAA GGACCATTCC AATCTGACGC CCTGCTAGTT 
CCCGAGGGCT GTCTGTTCGA CCACATCCAC AACGCCTCCC GATGCTGGCC ATTTGTGAGG 
TGGAACCAAA CCGGAGCAGC CGCTTGCCAG GAGCGCGGAA TGCAGATGCG CAGCTTCGCC 
ATGCTTCTGC CCTGCGGAAT CTCGGTCTTC TCCGGAGTGG AGTTCGTCTG CTGTCCCAAG 
CACTTCAAAA CCGATGAAAT TCATGTAAAG AAGACCGACT TGCCGGTGAT GCCGGCAGCT 
CAGATCAATA GCGCCAATGA CGAGCTGGTG ATGAATGACG AAGACGACAG CAACGACTCG 
AACTACTCGA AGGATGCCAA TGAAGACGAT CTGGACGATG AGGATGATTT GATGGGTGAT 
GATGAGGAGG ACGATATGGT GGCGGATGAG GCAGCTACCG CAGGCGGAAG CCCCAACACC 
GGTTCCTCAG GTGACTCAAA TAGCGGATCT TTGGATGACA TAAATGCGGA GTACGATAGC 
GGAGAGGAGG GTGACAACTA CGAGGAGGAC GGTGCTGGTT CCGAAAGCGA GGCTGAGGTT 
GAGGCCTCCT GGGATCAGAG CGGAGGGGCG AAGGTTGTGT CCCTAAAAAG CGATTCCTCA 
TCCCCGTCTA GCGCTCCGGT TGCTCCCGCC CCCGAGAAGG CACCTGTGAA GTCCGAATCG 
GTCACGAGCA CTCCTCAGCT GTCTGCCTCT GCCGCTGCTT TTGTAGCTGC CAATTCTGGA 
AACTCGGGAA CCGGAGCCGG AGCACCACCA TCCACTGCAC AACCCACATC GGATCCCTAC 
TTCACCCATT TTGATCCGCA CTACGAGCAC CAGAGCTACA AAGTAAGTCA GAAAGAAGCC 
CAACAGCGCC TTGAGGAATC GCACCGCGAG AAGGTCACGC GCGTTATGAA GGACTGGTCC 
GATCTCGAGG AGAAGTACCA GGACATGCGC CTAGCGGATC CCAAGGCAGC CCAGTCATTT 
AAGCAGCGGA TGACGGCTCG CTTCCAGGCA CTCGAGGAAG AAGGCAACGC CGAGAAGCAC 
CAGCTGGCCG CCATGCACCA GCAGCGCGTT TTGGCCCACA TCAACCAGCG GAAGCGCGAG 
GCCATGACCT GCTACACCCA GGCCCTGACT GAGCAGCCCC CGAATGCCCA CCACGTTGAG 
AAGTGTCTAC AGAAGCTGCT GCGCGCCCTG CACAAGGACC GGGCCCACGC CTTGGCCCAC 
TACCGGCACC TATTGAACTC TGGAGGACCT GGTGGACTAG AAGCAGCTGC TTCGGAGCGT 
CCACGCACGC TGGAGCGCCT AATCGACATC GATAGAGCCG TAAACCAGTC GATGACCATG 
CTCAAGCGCT ATCCGGAGCT CTCCGCCAAG ATTGCCCAGC TGATGAATGA CTACATTCTG 
GCACTGCGCA GCAAGGATGA CATTCCCGGC TCCTCGCTGG GAATGAGCGA GGAGGCGGAG 
GCCGGCATTC TGGACAAGTA CCGCGTCGAG ATCGAGCGCA AGGTGGCCGA GAAAGAGCGA 
CTACGGCTGG CGGAGAAGCA GCGCAAGGAG CAGCGTGCCG CTGAGCGGGA AAAACTGCGC 
GAGGAGAAGC TGCGCCTGGA GGCCAAAAAG GTGGACGACA TGCTCAAGTC ACAGGTGGCC 
GAGCAGCAGT CCCAGCCGAC CCAGTCGTCA ACCCAATCGC AGGCGCAGCA GCAGCAGCAG 
GAAAAGAGCC TTCCCGGCAA GGAGCTGGGT CCCGACGCAG CTCTGGTCAC AGCGGCCAAT 
CCCAACCTAG AGACCACCAA AAGCGAGAAG GATCTGTCGG ACACTGAGTA TGGGGAGGCA 
ACAGTGAGCA GCACCAAGGT GCAGACCGTG CTGCCAACGG TCGACGACGA TGCCGTCCAG 
CGGGCAGTGG AGGACGTAGC CGCCGCCGTC GCCCACCAGG AGGCCGAGCC GCAGGTGCAG 
CACTTCATGA CCCACGACCT GGGCCACCGC GAATCGAGCT TCTCGCTGCG CCGCGAGTTC 
GCGCAACATG CACACGCGGC CAAAGAGGGC CGCAACGTCT ACTTCACGCT CTCCTTCGCC 
GGGATCGCCC TGATGGCGGC CGTTTTCGTT GGCGTGGCCG TGGCCAAATG GCGAACATCG 
CGCTCGCCGC ACGCCCAGGG CTTCATCGAG GTCGACCAGA ACGTCACAAC ACACCATCCC 
ATTGTGCGCG AGGAGAAGAT CGTGCCCAAC ATGCAGATCA ATGGGTACGA GAATCCGACC 
TACAAGTATT TCGAAGTGAA AGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Appl ( NM_001258523.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245452.1 Drosophila melanogaster beta amyloid protein precursor-like (Appl), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa03264
Clone ID Related Accession (Same CDS sequence)	NM_001258523.2
Accession Version	NM_001258523.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	beta amyloid protein precursor-like, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001258523.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGCGCCG CGCTGCGTCG TAATTTGTTG CTGAGGAGCC TCTGGGTCGT CCTGGCCATT 
GGCACTGCTC AGGTTCAGGC CGCATCGCCG CGATGGGAGC CCCAGATTGC CGTTCTCTGT 
GAGGCCGGAC AAATCTACCA GCCGCAGTAT CTTTCCGAGG AGGGACGCTG GGTGACGGAC 
CTTAGCAAGA AGACAACCGG TCCCACATGT TTGCGTGATA AAATGGATTT GCTTGATTAC 
TGCAAGAAGG CCTATCCCAA CCGAGACATA ACCAACATTG TGGAGTCGTC CCACTACCAG 
AAGATCGGCG GCTGGTGTCG CCAGGGGGCA TTGAATGCGG CTAAGTGCAA GGGATCCCAC 
CGATGGATCA AGCCATTCCG CTGTCTCGAA GGACCATTCC AATCTGACGC CCTGCTAGTT 
CCCGAGGGCT GTCTGTTCGA CCACATCCAC AACGCCTCCC GATGCTGGCC ATTTGTGAGG 
TGGAACCAAA CCGGAGCAGC CGCTTGCCAG GAGCGCGGAA TGCAGATGCG CAGCTTCGCC 
ATGCTTCTGC CCTGCGGAAT CTCGGTCTTC TCCGGAGTGG AGTTCGTCTG CTGTCCCAAG 
CACTTCAAAA CCGATGAAAT TCATGTAAAG AAGACCGACT TGCCGGTGAT GCCGGCAGCT 
CAGATCAATA GCGCCAATGA CGAGCTGGTG ATGAATGACG AAGACGACAG CAACGACTCG 
AACTACTCGA AGGATGCCAA TGAAGACGAT CTGGACGATG AGGATGATTT GATGGGTGAT 
GATGAGGAGG ACGATATGGT GGCGGATGAG GCAGCTACCG CAGGCGGAAG CCCCAACACC 
GGTTCCTCAG GTGACTCAAA TAGCGGATCT TTGGATGACA TAAATGCGGA GTACGATAGC 
GGAGAGGAGG GTGACAACTA CGAGGAGGAC GGTGCTGGTT CCGAAAGCGA GGCTGAGGTT 
GAGGCCTCCT GGGATCAGAG CGGAGGGGCG AAGGTTGTGT CCCTAAAAAG CGATTCCTCA 
TCCCCGTCTA GCGCTCCGGT TGCTCCCGCC CCCGAGAAGG CACCTGTGAA GTCCGAATCG 
GTCACGAGCA CTCCTCAGCT GTCTGCCTCT GCCGCTGCTT TTGTAGCTGC CAATTCTGGA 
AACTCGGGAA CCGGAGCCGG AGCACCACCA TCCACTGCAC AACCCACATC GGATCCCTAC 
TTCACCCATT TTGATCCGCA CTACGAGCAC CAGAGCTACA AAGAAGCCCA ACAGCGCCTT 
GAGGAATCGC ACCGCGAGAA GGTCACGCGC GTTATGAAGG ACTGGTCCGA TCTCGAGGAG 
AAGTACCAGG ACATGCGCCT AGCGGATCCC AAGGCAGCCC AGTCATTTAA GCAGCGGATG 
ACGGCTCGCT TCCAGACTTC TGTTCAGGCA CTCGAGGAAG AAGGCAACGC CGAGAAGCAC 
CAGCTGGCCG CCATGCACCA GCAGCGCGTT TTGGCCCACA TCAACCAGCG GAAGCGCGAG 
GCCATGACCT GCTACACCCA GGCCCTGACT GAGCAGCCCC CGAATGCCCA CCACGTTGAG 
AAGTGTCTAC AGAAGCTGCT GCGCGCCCTG CACAAGGACC GGGCCCACGC CTTGGCCCAC 
TACCGGCACC TATTGAACTC TGGAGGACCT GGTGGACTAG AAGCAGCTGC TTCGGAGCGT 
CCACGCACGC TGGAGCGCCT AATCGACATC GATAGAGCCG TAAACCAGTC GATGACCATG 
CTCAAGCGCT ATCCGGAGCT CTCCGCCAAG ATTGCCCAGC TGATGAATGA CTACATTCTG 
GCACTGCGCA GCAAGGATGA CATTCCCGGC TCCTCGCTGG GAATGAGCGA GGAGGCGGAG 
GCCGGCATTC TGGACAAGTA CCGCGTCGAG ATCGAGCGCA AGGTGGCCGA GAAAGAGCGA 
CTACGGCTGG CGGAGAAGCA GCGCAAGGAG CAGCGTGCCG CTGAGCGGGA AAAACTGCGC 
GAGGAGAAGC TGCGCCTGGA GGCCAAAAAG GTGGACGACA TGCTCAAGTC ACAGGTGGCC 
GAGCAGCAGT CCCAGCCGAC CCAGTCGTCA ACCCAATCGC AGGCGCAGCA GCAGCAGCAG 
GAAAAGAGCC TTCCCGGCAA GGAGCTGGGT CCCGACGCAG CTCTGGTCAC AGCGGCCAAT 
CCCAACCTAG AGACCACCAA AAGCGAGAAG GATCTGTCGG ACACTGAGTA TGGGGAGGCA 
ACAGTGAGCA GCACCAAGGT GCAGACCGTG CTGCCAACGG TCGACGACGA TGCCGTCCAG 
CGGGCAGTGG AGGACGTAGC CGCCGCCGTC GCCCACCAGG AGGCCGAGCC GCAGGTGCAG 
CACTTCATGA CCCACGACCT GGGCCACCGC GAATCGAGCT TCTCGCTGCG CCGCGAGTTC 
GCGCAACATG CACACGCGGC CAAAGAGGGC CGCAACGTCT ACTTCACGCT CTCCTTCGCC 
GGGATCGCCC TGATGGCGGC CGTTTTCGTT GGCGTGGCCG TGGCCAAATG GCGAACATCG 
CGCTCGCCGC ACGCCCAGGG CTTCATCGAG GTCGACCAGA ACGTCACAAC ACACCATCCC 
ATTGTGCGCG AGGAGAAGAT CGTGCCCAAC ATGCAGATCA ATGGGTACGA GAATCCGACC 
TACAAGTATT TCGAAGTGAA AGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245452.1
CDS	207..2873
Translation	MCAALRRNLLLRSLWVVLAIGTAQVQAASPRWEPQIAVLCEAGQIYQPQYLSEEGRWVTDLSKKTTGPTCLRDKMDLLDYCKKAYPNRDITNIVESSHYQKIGGWCRQGALNAAKCKGSHRWIKPFRCLEGPFQSDALLVPEGCLFDHIHNASRCWPFVRWNQTGAAACQERGMQMRSFAMLLPCGISVFSGVEFVCCPKHFKTDEIHVKKTDLPVMPAAQINSANDELVMNDEDDSNDSNYSKDANEDDLDDEDDLMGDDEEDDMVADEAATAGGSPNTGSSGDSNSGSLDDINAEYDSGEEGDNYEEDGAGSESEAEVEASWDQSGGAKVVSLKSDSSSPSSAPVAPAPEKAPVKSESVTSTPQLSASAAAFVAANSGNSGTGAGAPPSTAQPTSDPYFTHFDPHYEHQSYKEAQQRLEESHREKVTRVMKDWSDLEEKYQDMRLADPKAAQSFKQRMTARFQTSVQALEEEGNAEKHQLAAMHQQRVLAHINQRKREAMTCYTQALTEQPPNAHHVEKCLQKLLRALHKDRAHALAHYRHLLNSGGPGGLEAAASERPRTLERLIDIDRAVNQSMTMLKRYPELSAKIAQLMNDYILALRSKDDIPGSSLGMSEEAEAGILDKYRVEIERKVAEKERLRLAEKQRKEQRAAEREKLREEKLRLEAKKVDDMLKSQVAEQQSQPTQSSTQSQAQQQQQEKSLPGKELGPDAALVTAANPNLETTKSEKDLSDTEYGEATVSSTKVQTVLPTVDDDAVQRAVEDVAAAVAHQEAEPQVQHFMTHDLGHRESSFSLRREFAQHAHAAKEGRNVYFTLSFAGIALMAAVFVGVAVAKWRTSRSPHAQGFIEVDQNVTTHHPIVREEKIVPNMQINGYENPTYKYFEVKE

Target ORF information:

RefSeq Version	NM_001258523.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster beta amyloid protein precursor-like (Appl), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258523.2

ORF Insert Sequence:

ATGTGCGCCG CGCTGCGTCG TAATTTGTTG CTGAGGAGCC TCTGGGTCGT CCTGGCCATT 
GGCACTGCTC AGGTTCAGGC CGCATCGCCG CGATGGGAGC CCCAGATTGC CGTTCTCTGT 
GAGGCCGGAC AAATCTACCA GCCGCAGTAT CTTTCCGAGG AGGGACGCTG GGTGACGGAC 
CTTAGCAAGA AGACAACCGG TCCCACATGT TTGCGTGATA AAATGGATTT GCTTGATTAC 
TGCAAGAAGG CCTATCCCAA CCGAGACATA ACCAACATTG TGGAGTCGTC CCACTACCAG 
AAGATCGGCG GCTGGTGTCG CCAGGGGGCA TTGAATGCGG CTAAGTGCAA GGGATCCCAC 
CGATGGATCA AGCCATTCCG CTGTCTCGAA GGACCATTCC AATCTGACGC CCTGCTAGTT 
CCCGAGGGCT GTCTGTTCGA CCACATCCAC AACGCCTCCC GATGCTGGCC ATTTGTGAGG 
TGGAACCAAA CCGGAGCAGC CGCTTGCCAG GAGCGCGGAA TGCAGATGCG CAGCTTCGCC 
ATGCTTCTGC CCTGCGGAAT CTCGGTCTTC TCCGGAGTGG AGTTCGTCTG CTGTCCCAAG 
CACTTCAAAA CCGATGAAAT TCATGTAAAG AAGACCGACT TGCCGGTGAT GCCGGCAGCT 
CAGATCAATA GCGCCAATGA CGAGCTGGTG ATGAATGACG AAGACGACAG CAACGACTCG 
AACTACTCGA AGGATGCCAA TGAAGACGAT CTGGACGATG AGGATGATTT GATGGGTGAT 
GATGAGGAGG ACGATATGGT GGCGGATGAG GCAGCTACCG CAGGCGGAAG CCCCAACACC 
GGTTCCTCAG GTGACTCAAA TAGCGGATCT TTGGATGACA TAAATGCGGA GTACGATAGC 
GGAGAGGAGG GTGACAACTA CGAGGAGGAC GGTGCTGGTT CCGAAAGCGA GGCTGAGGTT 
GAGGCCTCCT GGGATCAGAG CGGAGGGGCG AAGGTTGTGT CCCTAAAAAG CGATTCCTCA 
TCCCCGTCTA GCGCTCCGGT TGCTCCCGCC CCCGAGAAGG CACCTGTGAA GTCCGAATCG 
GTCACGAGCA CTCCTCAGCT GTCTGCCTCT GCCGCTGCTT TTGTAGCTGC CAATTCTGGA 
AACTCGGGAA CCGGAGCCGG AGCACCACCA TCCACTGCAC AACCCACATC GGATCCCTAC 
TTCACCCATT TTGATCCGCA CTACGAGCAC CAGAGCTACA AAGAAGCCCA ACAGCGCCTT 
GAGGAATCGC ACCGCGAGAA GGTCACGCGC GTTATGAAGG ACTGGTCCGA TCTCGAGGAG 
AAGTACCAGG ACATGCGCCT AGCGGATCCC AAGGCAGCCC AGTCATTTAA GCAGCGGATG 
ACGGCTCGCT TCCAGACTTC TGTTCAGGCA CTCGAGGAAG AAGGCAACGC CGAGAAGCAC 
CAGCTGGCCG CCATGCACCA GCAGCGCGTT TTGGCCCACA TCAACCAGCG GAAGCGCGAG 
GCCATGACCT GCTACACCCA GGCCCTGACT GAGCAGCCCC CGAATGCCCA CCACGTTGAG 
AAGTGTCTAC AGAAGCTGCT GCGCGCCCTG CACAAGGACC GGGCCCACGC CTTGGCCCAC 
TACCGGCACC TATTGAACTC TGGAGGACCT GGTGGACTAG AAGCAGCTGC TTCGGAGCGT 
CCACGCACGC TGGAGCGCCT AATCGACATC GATAGAGCCG TAAACCAGTC GATGACCATG 
CTCAAGCGCT ATCCGGAGCT CTCCGCCAAG ATTGCCCAGC TGATGAATGA CTACATTCTG 
GCACTGCGCA GCAAGGATGA CATTCCCGGC TCCTCGCTGG GAATGAGCGA GGAGGCGGAG 
GCCGGCATTC TGGACAAGTA CCGCGTCGAG ATCGAGCGCA AGGTGGCCGA GAAAGAGCGA 
CTACGGCTGG CGGAGAAGCA GCGCAAGGAG CAGCGTGCCG CTGAGCGGGA AAAACTGCGC 
GAGGAGAAGC TGCGCCTGGA GGCCAAAAAG GTGGACGACA TGCTCAAGTC ACAGGTGGCC 
GAGCAGCAGT CCCAGCCGAC CCAGTCGTCA ACCCAATCGC AGGCGCAGCA GCAGCAGCAG 
GAAAAGAGCC TTCCCGGCAA GGAGCTGGGT CCCGACGCAG CTCTGGTCAC AGCGGCCAAT 
CCCAACCTAG AGACCACCAA AAGCGAGAAG GATCTGTCGG ACACTGAGTA TGGGGAGGCA 
ACAGTGAGCA GCACCAAGGT GCAGACCGTG CTGCCAACGG TCGACGACGA TGCCGTCCAG 
CGGGCAGTGG AGGACGTAGC CGCCGCCGTC GCCCACCAGG AGGCCGAGCC GCAGGTGCAG 
CACTTCATGA CCCACGACCT GGGCCACCGC GAATCGAGCT TCTCGCTGCG CCGCGAGTTC 
GCGCAACATG CACACGCGGC CAAAGAGGGC CGCAACGTCT ACTTCACGCT CTCCTTCGCC 
GGGATCGCCC TGATGGCGGC CGTTTTCGTT GGCGTGGCCG TGGCCAAATG GCGAACATCG 
CGCTCGCCGC ACGCCCAGGG CTTCATCGAG GTCGACCAGA ACGTCACAAC ACACCATCCC 
ATTGTGCGCG AGGAGAAGAT CGTGCCCAAC ATGCAGATCA ATGGGTACGA GAATCCGACC 
TACAAGTATT TCGAAGTGAA AGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Appl ( NM_001258520.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245449.1 Drosophila melanogaster beta amyloid protein precursor-like (Appl), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa03265
Clone ID Related Accession (Same CDS sequence)	NM_001258520.2
Accession Version	NM_001258520.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	beta amyloid protein precursor-like, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001258520.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGCGCCG CGCTGCGTCG TAATTTGTTG CTGAGGAGCC TCTGGGTCGT CCTGGCCATT 
GGCACTGCTC AGGTTCAGGC CTATCCCAAC CGAGACATAA CCAACATTGT GGAGTCGTCC 
CACTACCAGA AGATCGGCGG CTGGTGTCGC CAGGGGGCAT TGAATGCGGC TAAGTGCAAG 
GGATCCCACC GATGGATCAA GCCATTCCGC TGTCTCGGAC CATTCCAATC TGACGCCCTG 
CTAGTTCCCG AGGGCTGTCT GTTCGACCAC ATCCACAACG CCTCCCGATG CTGGCCATTT 
GTGAGGTGGA ACCAAACCGG AGCAGCCGCT TGCCAGGAGC GCGGAATGCA GATGCGCAGC 
TTCGCCATGC TTCTGCCCTG CGGAATCTCG GTCTTCTCCG GAGTGGAGTT CGTCTGCTGT 
CCCAAGCACT TCAAAACCGA TGAAATTCAT GTAAAGAAGA CCGACTTGCC GGTGATGCCG 
GCAGCTCAGA TCAATAGCGC CAATGACGAG CTGGTGATGA ATGACGAAGA CGACAGCAAC 
GACTCGAACT ACTCGAAGGA TGCCAATGAA GACGATCTGG ACGATGAGGA TGATTTGATG 
GGTGATGATG AGGAGGACGA TATGGTGGCG GATGAGGCAG CTACCGCAGG CGGAAGCCCC 
AACACCGGTT CCTCAGGTGA CTCAAATAGC GGATCTTTGG ATGACATAAA TGCGGAGTAC 
GATAGCGGAG AGGAGGGTGA CAACTACGAG GAGGACGGTG CTGGTTCCGA AAGCGAGGCT 
GAGGTTGAGG CCTCCTGGGA TCAGAGCGGA GGGGCGAAGG TTGTGTCCCT AAAAAGCGAT 
TCCTCATCCC CGTCTAGCGC TCCGGTTGCT CCCGCCCCCG AGAAGGCACC TGTGAAGTCC 
GAATCGGTCA CGAGCACTCC TCAGCTGTCT GCCTCTGCCG CTGCTTTTGT AGCTGCCAAT 
TCTGGAAACT CGGGAACCGG AGCCGGAGCA CCACCATCCA CTGCACAACC CACATCGGAT 
CCCTACTTCA CCCATTTTGA TCCGCACTAC GAGCACCAGA GCTACAAAGT AAGTCAGAAA 
CGCCTTGAGG AATCGCACCG CGAGAAGGTC ACGCGCGTTA TGAAGGACTG GTCCGATCTC 
GAGGAGAAGT ACCAGGACAT GCGCCTAGCG GATCCCAAGG CAGCCCAGTC ATTTAAGCAG 
CGGATGACGG CTCGCTTCCA GACTTCTGTT CAGGCACTCG AGGAAGAAGG CAACGCCGAG 
AAGCACCAGC TGGCCGCCAT GCACCAGCAG CGCGTTTTGG CCCACATCAA CCAGCGGAAG 
CGCGAGGCCA TGACCTGCTA CACCCAGGCC CTGACTGAGC AGCCCCCGAA TGCCCACCAC 
GTTGAGAAGT GTCTACAGAA GCTGCTGCGC GCCCTGCACA AGGACCGGGC CCACGCCTTG 
GCCCACTACC GGCACCTATT GAACTCTGGA GGACCTGGTG GACTAGAAGC AGCTGCTTCG 
GAGCGTCCAC GCACGCTGGA GCGCCTAATC GACATCGATA GAGCCGTAAA CCAGTCGATG 
ACCATGCTCA AGCGCTATCC GGAGCTCTCC GCCAAGATTG CCCAGCTGAT GAATGACTAC 
ATTCTGGCAC TGCGCAGCAA GGATGACATT CCCGGCTCCT CGCTGGGAAT GAGCGAGGAG 
GCGGAGGCCG GCATTCTGGA CAAGTACCGC GTCGAGATCG AGCGCAAGGT GGCCGAGAAA 
GAGCGACTAC GGCTGGCGGA GAAGCAGCGC AAGGAGCAGC GTGCCGCTGA GCGGGAAAAA 
CTGCGCGAGG AGAAGCTGCG CCTGGAGGCC AAAAAGGTGG ACGACATGCT CAAGTCACAG 
GTGGCCGAGC AGCAGTCCCA GCCGACCCAG TCGTCAACCC AATCGCAGGC GCAGCAGCAG 
CAGCAGGAAA AGAGCCTTCC CGGCAAGGAG CTGGGTCCCG ACGCAGCTCT GGTCACAGCG 
GCCAATCCCA ACCTAGAGAC CACCAAAAGC GAGAAGGATC TGTCGGACAC TGAGTATGGG 
GAGGCAACAG TGAGCAGCAC CAAGGTGCAG ACCGTGCTGC CAACGGTCGA CGACGATGCC 
GTCCAGCGGG CAGTGGAGGA CGTAGCCGCC GCCGTCGCCC ACCAGGAGGC CGAGCCGCAG 
GTGCAGCACT TCATGACCCA CGACCTGGGC CACCGCGAAT CGAGCTTCTC GCTGCGCCGC 
GAGTTCGCGC AACATGCACA CGCGGCCAAA GAGGGCCGCA ACGTCTACTT CACGCTCTCC 
TTCGCCGGGA TCGCCCTGAT GGCGGCCGTT TTCGTTGGCG TGGCCGTGGC CAAATGGCGA 
ACATCGCGCT CGCCGCACGC CCAGGGCTTC ATCGAGGTCG ACCAGAACGT CACAACACAC 
CATCCCATTG TGCGCGAGGA GAAGATCGTG CCCAACATGC AGATCAATGG GTACGAGAAT 
CCGACCTACA AGTATTTCGA AGTGAAAGAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245449.1
CDS	207..2699
Translation	MCAALRRNLLLRSLWVVLAIGTAQVQAYPNRDITNIVESSHYQKIGGWCRQGALNAAKCKGSHRWIKPFRCLGPFQSDALLVPEGCLFDHIHNASRCWPFVRWNQTGAAACQERGMQMRSFAMLLPCGISVFSGVEFVCCPKHFKTDEIHVKKTDLPVMPAAQINSANDELVMNDEDDSNDSNYSKDANEDDLDDEDDLMGDDEEDDMVADEAATAGGSPNTGSSGDSNSGSLDDINAEYDSGEEGDNYEEDGAGSESEAEVEASWDQSGGAKVVSLKSDSSSPSSAPVAPAPEKAPVKSESVTSTPQLSASAAAFVAANSGNSGTGAGAPPSTAQPTSDPYFTHFDPHYEHQSYKVSQKRLEESHREKVTRVMKDWSDLEEKYQDMRLADPKAAQSFKQRMTARFQTSVQALEEEGNAEKHQLAAMHQQRVLAHINQRKREAMTCYTQALTEQPPNAHHVEKCLQKLLRALHKDRAHALAHYRHLLNSGGPGGLEAAASERPRTLERLIDIDRAVNQSMTMLKRYPELSAKIAQLMNDYILALRSKDDIPGSSLGMSEEAEAGILDKYRVEIERKVAEKERLRLAEKQRKEQRAAEREKLREEKLRLEAKKVDDMLKSQVAEQQSQPTQSSTQSQAQQQQQEKSLPGKELGPDAALVTAANPNLETTKSEKDLSDTEYGEATVSSTKVQTVLPTVDDDAVQRAVEDVAAAVAHQEAEPQVQHFMTHDLGHRESSFSLRREFAQHAHAAKEGRNVYFTLSFAGIALMAAVFVGVAVAKWRTSRSPHAQGFIEVDQNVTTHHPIVREEKIVPNMQINGYENPTYKYFEVKE

Target ORF information:

RefSeq Version	NM_001258520.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster beta amyloid protein precursor-like (Appl), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258520.2

ORF Insert Sequence:

ATGTGCGCCG CGCTGCGTCG TAATTTGTTG CTGAGGAGCC TCTGGGTCGT CCTGGCCATT 
GGCACTGCTC AGGTTCAGGC CTATCCCAAC CGAGACATAA CCAACATTGT GGAGTCGTCC 
CACTACCAGA AGATCGGCGG CTGGTGTCGC CAGGGGGCAT TGAATGCGGC TAAGTGCAAG 
GGATCCCACC GATGGATCAA GCCATTCCGC TGTCTCGGAC CATTCCAATC TGACGCCCTG 
CTAGTTCCCG AGGGCTGTCT GTTCGACCAC ATCCACAACG CCTCCCGATG CTGGCCATTT 
GTGAGGTGGA ACCAAACCGG AGCAGCCGCT TGCCAGGAGC GCGGAATGCA GATGCGCAGC 
TTCGCCATGC TTCTGCCCTG CGGAATCTCG GTCTTCTCCG GAGTGGAGTT CGTCTGCTGT 
CCCAAGCACT TCAAAACCGA TGAAATTCAT GTAAAGAAGA CCGACTTGCC GGTGATGCCG 
GCAGCTCAGA TCAATAGCGC CAATGACGAG CTGGTGATGA ATGACGAAGA CGACAGCAAC 
GACTCGAACT ACTCGAAGGA TGCCAATGAA GACGATCTGG ACGATGAGGA TGATTTGATG 
GGTGATGATG AGGAGGACGA TATGGTGGCG GATGAGGCAG CTACCGCAGG CGGAAGCCCC 
AACACCGGTT CCTCAGGTGA CTCAAATAGC GGATCTTTGG ATGACATAAA TGCGGAGTAC 
GATAGCGGAG AGGAGGGTGA CAACTACGAG GAGGACGGTG CTGGTTCCGA AAGCGAGGCT 
GAGGTTGAGG CCTCCTGGGA TCAGAGCGGA GGGGCGAAGG TTGTGTCCCT AAAAAGCGAT 
TCCTCATCCC CGTCTAGCGC TCCGGTTGCT CCCGCCCCCG AGAAGGCACC TGTGAAGTCC 
GAATCGGTCA CGAGCACTCC TCAGCTGTCT GCCTCTGCCG CTGCTTTTGT AGCTGCCAAT 
TCTGGAAACT CGGGAACCGG AGCCGGAGCA CCACCATCCA CTGCACAACC CACATCGGAT 
CCCTACTTCA CCCATTTTGA TCCGCACTAC GAGCACCAGA GCTACAAAGT AAGTCAGAAA 
CGCCTTGAGG AATCGCACCG CGAGAAGGTC ACGCGCGTTA TGAAGGACTG GTCCGATCTC 
GAGGAGAAGT ACCAGGACAT GCGCCTAGCG GATCCCAAGG CAGCCCAGTC ATTTAAGCAG 
CGGATGACGG CTCGCTTCCA GACTTCTGTT CAGGCACTCG AGGAAGAAGG CAACGCCGAG 
AAGCACCAGC TGGCCGCCAT GCACCAGCAG CGCGTTTTGG CCCACATCAA CCAGCGGAAG 
CGCGAGGCCA TGACCTGCTA CACCCAGGCC CTGACTGAGC AGCCCCCGAA TGCCCACCAC 
GTTGAGAAGT GTCTACAGAA GCTGCTGCGC GCCCTGCACA AGGACCGGGC CCACGCCTTG 
GCCCACTACC GGCACCTATT GAACTCTGGA GGACCTGGTG GACTAGAAGC AGCTGCTTCG 
GAGCGTCCAC GCACGCTGGA GCGCCTAATC GACATCGATA GAGCCGTAAA CCAGTCGATG 
ACCATGCTCA AGCGCTATCC GGAGCTCTCC GCCAAGATTG CCCAGCTGAT GAATGACTAC 
ATTCTGGCAC TGCGCAGCAA GGATGACATT CCCGGCTCCT CGCTGGGAAT GAGCGAGGAG 
GCGGAGGCCG GCATTCTGGA CAAGTACCGC GTCGAGATCG AGCGCAAGGT GGCCGAGAAA 
GAGCGACTAC GGCTGGCGGA GAAGCAGCGC AAGGAGCAGC GTGCCGCTGA GCGGGAAAAA 
CTGCGCGAGG AGAAGCTGCG CCTGGAGGCC AAAAAGGTGG ACGACATGCT CAAGTCACAG 
GTGGCCGAGC AGCAGTCCCA GCCGACCCAG TCGTCAACCC AATCGCAGGC GCAGCAGCAG 
CAGCAGGAAA AGAGCCTTCC CGGCAAGGAG CTGGGTCCCG ACGCAGCTCT GGTCACAGCG 
GCCAATCCCA ACCTAGAGAC CACCAAAAGC GAGAAGGATC TGTCGGACAC TGAGTATGGG 
GAGGCAACAG TGAGCAGCAC CAAGGTGCAG ACCGTGCTGC CAACGGTCGA CGACGATGCC 
GTCCAGCGGG CAGTGGAGGA CGTAGCCGCC GCCGTCGCCC ACCAGGAGGC CGAGCCGCAG 
GTGCAGCACT TCATGACCCA CGACCTGGGC CACCGCGAAT CGAGCTTCTC GCTGCGCCGC 
GAGTTCGCGC AACATGCACA CGCGGCCAAA GAGGGCCGCA ACGTCTACTT CACGCTCTCC 
TTCGCCGGGA TCGCCCTGAT GGCGGCCGTT TTCGTTGGCG TGGCCGTGGC CAAATGGCGA 
ACATCGCGCT CGCCGCACGC CCAGGGCTTC ATCGAGGTCG ACCAGAACGT CACAACACAC 
CATCCCATTG TGCGCGAGGA GAAGATCGTG CCCAACATGC AGATCAATGG GTACGAGAAT 
CCGACCTACA AGTATTTCGA AGTGAAAGAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Appl ( NM_001258519.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245448.1 Drosophila melanogaster beta amyloid protein precursor-like (Appl), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa03266
Clone ID Related Accession (Same CDS sequence)	NM_001258519.1
Accession Version	NM_001258519.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2673bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	beta amyloid protein precursor-like, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTGCGCCG CGCTGCGTCG TAATTTGTTG CTGAGGAGCC TCTGGGTCGT CCTGGCCATT 
GGCACTGCTC AGGTTCAGGC CGCATCGCCG CGATGGGAGC CCCAGATTGC CGTTCTCTGT 
GAGGCCGGAC AAATCTACCA GCCGCAGTAT CTTTCCGAGG AGGGACGCTG GGTGACGGAC 
CTTAGCAAGA AGACAACCGG TCCCACATGT TTGCGTGATA AAATGGATTT GCTTGATTAC 
TGCAAGAAGG CCTATCCCAA CCGAGACATA ACCAACATTG TGGAGTCGTC CCACTACCAG 
AAGATCGGCG GCTGGTGTCG CCAGGGGGCA TTGAATGCGG CTAAGTGCAA GGGATCCCAC 
CGATGGATCA AGCCATTCCG CTGTCTCGGA CCATTCCAAT CTGACGCCCT GCTAGTTCCC 
GAGGGCTGTC TGTTCGACCA CATCCACAAC GCCTCCCGAT GCTGGCCATT TGTGAGGTGG 
AACCAAACCG GAGCAGCCGC TTGCCAGGAG CGCGGAATGC AGATGCGCAG CTTCGCCATG 
CTTCTGCCCT GCGGAATCTC GGTCTTCTCC GGAGTGGAGT TCGTCTGCTG TCCCAAGCAC 
TTCAAAACCG ATGAAATTCA TGTAAAGAAG ACCGACTTGC CGGTGATGCC GGCAGCTCAG 
ATCAATAGCG CCAATGACGA GCTGGTGATG AATGACGAAG ACGACAGCAA CGACTCGAAC 
TACTCGAAGG ATGCCAATGA AGACGATCTG GACGATGAGG ATGATTTGAT GGGTGATGAT 
GAGGAGGACG ATATGGTGGC GGATGAGGCA GCTACCGCAG GCGGAAGCCC CAACACCGGT 
TCCTCAGGTG ACTCAAATAG CGGATCTTTG GATGACATAA ATGCGGAGTA CGATAGCGGA 
GAGGAGGGTG ACAACTACGA GGAGGACGGT GCTGGTTCCG AAAGCGAGGC TGAGGTTGAG 
GCCTCCTGGG ATCAGAGCGG AGGGGCGAAG GTTGTGTCCC TAAAAAGCGA TTCCTCATCC 
CCGTCTAGCG CTCCGGTTGC TCCCGCCCCC GAGAAGGCAC CTGTGAAGTC CGAATCGGTC 
ACGAGCACTC CTCAGCTGTC TGCCTCTGCC GCTGCTTTTG TAGCTGCCAA TTCTGGAAAC 
TCGGGAACCG GAGCCGGAGC ACCACCATCC ACTGCACAAC CCACATCGGA TCCCTACTTC 
ACCCATTTTG ATCCGCACTA CGAGCACCAG AGCTACAAAG TAAGTCAGAA ACGCCTTGAG 
GAATCGCACC GCGAGAAGGT CACGCGCGTT ATGAAGGACT GGTCCGATCT CGAGGAGAAG 
TACCAGGACA TGCGCCTAGC GGATCCCAAG GCAGCCCAGT CATTTAAGCA GCGGATGACG 
GCTCGCTTCC AGACTTCTGT TCAGGCACTC GAGGAAGAAG GCAACGCCGA GAAGCACCAG 
CTGGCCGCCA TGCACCAGCA GCGCGTTTTG GCCCACATCA ACCAGCGGAA GCGCGAGGCC 
ATGACCTGCT ACACCCAGGC CCTGACTGAG CAGCCCCCGA ATGCCCACCA CGTTGAGAAG 
TGTCTACAGA AGCTGCTGCG CGCCCTGCAC AAGGACCGGG CCCACGCCTT GGCCCACTAC 
CGGCACCTAT TGAACTCTGG AGGACCTGGT GGACTAGAAG CAGCTGCTTC GGAGCGTCCA 
CGCACGCTGG AGCGCCTAAT CGACATCGAT AGAGCCGTAA ACCAGTCGAT GACCATGCTC 
AAGCGCTATC CGGAGCTCTC CGCCAAGATT GCCCAGCTGA TGAATGACTA CATTCTGGCA 
CTGCGCAGCA AGGATGACAT TCCCGGCTCC TCGCTGGGAA TGAGCGAGGA GGCGGAGGCC 
GGCATTCTGG ACAAGTACCG CGTCGAGATC GAGCGCAAGG TGGCCGAGAA AGAGCGACTA 
CGGCTGGCGG AGAAGCAGCG CAAGGAGCAG CGTGCCGCTG AGCGGGAAAA ACTGCGCGAG 
GAGAAGCTGC GCCTGGAGGC CAAAAAGGTG GACGACATGC TCAAGTCACA GGTGGCCGAG 
CAGCAGTCCC AGCCGACCCA GTCGTCAACC CAATCGCAGG CGCAGCAGCA GCAGCAGGAA 
AAGAGCCTTC CCGGCAAGGA GCTGGGTCCC GACGCAGCTC TGGTCACAGC GGCCAATCCC 
AACCTAGAGA CCACCAAAAG CGAGAAGGAT CTGTCGGACA CTGAGTATGG GGAGGCAACA 
GTGAGCACCA AGGTGCAGAC CGTGCTGCCA ACGGTCGACG ACGATGCCGT CCAGCGGGCA 
GTGGAGGACG TAGCCGCCGC CGTCGCCCAC CAGGAGGCCG AGCCGCAGGT GCAGCACTTC 
ATGACCCACG ACCTGGGCCA CCGCGAATCG AGCTTCTCGC TGCGCCGCGA GTTCGCGCAA 
CATGCACACG CGGCCAAAGA GGGCCGCAAC GTCTACTTCA CGCTCTCCTT CGCCGGGATC 
GCCCTGATGG CGGCCGTTTT CGTTGGCGTG GCCGTGGCCA AATGGCGAAC ATCGCGCTCG 
CCGCACGCCC AGGGCTTCAT CGAGGTCGAC CAGGTGGGTT GTCAGAACGT CACAACACAC 
CATCCCATTG TGCGCGAGGA GAAGATCGTG CCCAACATGC AGATCAATGG GTACGAGAAT 
CCGACCTACA AGTATTTCGA AGTGAAAGAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245448.1
CDS	193..2865
Translation	MCAALRRNLLLRSLWVVLAIGTAQVQAASPRWEPQIAVLCEAGQIYQPQYLSEEGRWVTDLSKKTTGPTCLRDKMDLLDYCKKAYPNRDITNIVESSHYQKIGGWCRQGALNAAKCKGSHRWIKPFRCLGPFQSDALLVPEGCLFDHIHNASRCWPFVRWNQTGAAACQERGMQMRSFAMLLPCGISVFSGVEFVCCPKHFKTDEIHVKKTDLPVMPAAQINSANDELVMNDEDDSNDSNYSKDANEDDLDDEDDLMGDDEEDDMVADEAATAGGSPNTGSSGDSNSGSLDDINAEYDSGEEGDNYEEDGAGSESEAEVEASWDQSGGAKVVSLKSDSSSPSSAPVAPAPEKAPVKSESVTSTPQLSASAAAFVAANSGNSGTGAGAPPSTAQPTSDPYFTHFDPHYEHQSYKVSQKRLEESHREKVTRVMKDWSDLEEKYQDMRLADPKAAQSFKQRMTARFQTSVQALEEEGNAEKHQLAAMHQQRVLAHINQRKREAMTCYTQALTEQPPNAHHVEKCLQKLLRALHKDRAHALAHYRHLLNSGGPGGLEAAASERPRTLERLIDIDRAVNQSMTMLKRYPELSAKIAQLMNDYILALRSKDDIPGSSLGMSEEAEAGILDKYRVEIERKVAEKERLRLAEKQRKEQRAAEREKLREEKLRLEAKKVDDMLKSQVAEQQSQPTQSSTQSQAQQQQQEKSLPGKELGPDAALVTAANPNLETTKSEKDLSDTEYGEATVSTKVQTVLPTVDDDAVQRAVEDVAAAVAHQEAEPQVQHFMTHDLGHRESSFSLRREFAQHAHAAKEGRNVYFTLSFAGIALMAAVFVGVAVAKWRTSRSPHAQGFIEVDQVGCQNVTTHHPIVREEKIVPNMQINGYENPTYKYFEVKE

Target ORF information:

RefSeq Version	NM_001258519.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster beta amyloid protein precursor-like (Appl), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258519.1

ORF Insert Sequence:

ATGTGCGCCG CGCTGCGTCG TAATTTGTTG CTGAGGAGCC TCTGGGTCGT CCTGGCCATT 
GGCACTGCTC AGGTTCAGGC CGCATCGCCG CGATGGGAGC CCCAGATTGC CGTTCTCTGT 
GAGGCCGGAC AAATCTACCA GCCGCAGTAT CTTTCCGAGG AGGGACGCTG GGTGACGGAC 
CTTAGCAAGA AGACAACCGG TCCCACATGT TTGCGTGATA AAATGGATTT GCTTGATTAC 
TGCAAGAAGG CCTATCCCAA CCGAGACATA ACCAACATTG TGGAGTCGTC CCACTACCAG 
AAGATCGGCG GCTGGTGTCG CCAGGGGGCA TTGAATGCGG CTAAGTGCAA GGGATCCCAC 
CGATGGATCA AGCCATTCCG CTGTCTCGGA CCATTCCAAT CTGACGCCCT GCTAGTTCCC 
GAGGGCTGTC TGTTCGACCA CATCCACAAC GCCTCCCGAT GCTGGCCATT TGTGAGGTGG 
AACCAAACCG GAGCAGCCGC TTGCCAGGAG CGCGGAATGC AGATGCGCAG CTTCGCCATG 
CTTCTGCCCT GCGGAATCTC GGTCTTCTCC GGAGTGGAGT TCGTCTGCTG TCCCAAGCAC 
TTCAAAACCG ATGAAATTCA TGTAAAGAAG ACCGACTTGC CGGTGATGCC GGCAGCTCAG 
ATCAATAGCG CCAATGACGA GCTGGTGATG AATGACGAAG ACGACAGCAA CGACTCGAAC 
TACTCGAAGG ATGCCAATGA AGACGATCTG GACGATGAGG ATGATTTGAT GGGTGATGAT 
GAGGAGGACG ATATGGTGGC GGATGAGGCA GCTACCGCAG GCGGAAGCCC CAACACCGGT 
TCCTCAGGTG ACTCAAATAG CGGATCTTTG GATGACATAA ATGCGGAGTA CGATAGCGGA 
GAGGAGGGTG ACAACTACGA GGAGGACGGT GCTGGTTCCG AAAGCGAGGC TGAGGTTGAG 
GCCTCCTGGG ATCAGAGCGG AGGGGCGAAG GTTGTGTCCC TAAAAAGCGA TTCCTCATCC 
CCGTCTAGCG CTCCGGTTGC TCCCGCCCCC GAGAAGGCAC CTGTGAAGTC CGAATCGGTC 
ACGAGCACTC CTCAGCTGTC TGCCTCTGCC GCTGCTTTTG TAGCTGCCAA TTCTGGAAAC 
TCGGGAACCG GAGCCGGAGC ACCACCATCC ACTGCACAAC CCACATCGGA TCCCTACTTC 
ACCCATTTTG ATCCGCACTA CGAGCACCAG AGCTACAAAG TAAGTCAGAA ACGCCTTGAG 
GAATCGCACC GCGAGAAGGT CACGCGCGTT ATGAAGGACT GGTCCGATCT CGAGGAGAAG 
TACCAGGACA TGCGCCTAGC GGATCCCAAG GCAGCCCAGT CATTTAAGCA GCGGATGACG 
GCTCGCTTCC AGACTTCTGT TCAGGCACTC GAGGAAGAAG GCAACGCCGA GAAGCACCAG 
CTGGCCGCCA TGCACCAGCA GCGCGTTTTG GCCCACATCA ACCAGCGGAA GCGCGAGGCC 
ATGACCTGCT ACACCCAGGC CCTGACTGAG CAGCCCCCGA ATGCCCACCA CGTTGAGAAG 
TGTCTACAGA AGCTGCTGCG CGCCCTGCAC AAGGACCGGG CCCACGCCTT GGCCCACTAC 
CGGCACCTAT TGAACTCTGG AGGACCTGGT GGACTAGAAG CAGCTGCTTC GGAGCGTCCA 
CGCACGCTGG AGCGCCTAAT CGACATCGAT AGAGCCGTAA ACCAGTCGAT GACCATGCTC 
AAGCGCTATC CGGAGCTCTC CGCCAAGATT GCCCAGCTGA TGAATGACTA CATTCTGGCA 
CTGCGCAGCA AGGATGACAT TCCCGGCTCC TCGCTGGGAA TGAGCGAGGA GGCGGAGGCC 
GGCATTCTGG ACAAGTACCG CGTCGAGATC GAGCGCAAGG TGGCCGAGAA AGAGCGACTA 
CGGCTGGCGG AGAAGCAGCG CAAGGAGCAG CGTGCCGCTG AGCGGGAAAA ACTGCGCGAG 
GAGAAGCTGC GCCTGGAGGC CAAAAAGGTG GACGACATGC TCAAGTCACA GGTGGCCGAG 
CAGCAGTCCC AGCCGACCCA GTCGTCAACC CAATCGCAGG CGCAGCAGCA GCAGCAGGAA 
AAGAGCCTTC CCGGCAAGGA GCTGGGTCCC GACGCAGCTC TGGTCACAGC GGCCAATCCC 
AACCTAGAGA CCACCAAAAG CGAGAAGGAT CTGTCGGACA CTGAGTATGG GGAGGCAACA 
GTGAGCACCA AGGTGCAGAC CGTGCTGCCA ACGGTCGACG ACGATGCCGT CCAGCGGGCA 
GTGGAGGACG TAGCCGCCGC CGTCGCCCAC CAGGAGGCCG AGCCGCAGGT GCAGCACTTC 
ATGACCCACG ACCTGGGCCA CCGCGAATCG AGCTTCTCGC TGCGCCGCGA GTTCGCGCAA 
CATGCACACG CGGCCAAAGA GGGCCGCAAC GTCTACTTCA CGCTCTCCTT CGCCGGGATC 
GCCCTGATGG CGGCCGTTTT CGTTGGCGTG GCCGTGGCCA AATGGCGAAC ATCGCGCTCG 
CCGCACGCCC AGGGCTTCAT CGAGGTCGAC CAGGTGGGTT GTCAGAACGT CACAACACAC 
CATCCCATTG TGCGCGAGGA GAAGATCGTG CCCAACATGC AGATCAATGG GTACGAGAAT 
CCGACCTACA AGTATTTCGA AGTGAAAGAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Appl cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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