mld cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	mld
Entrez Gene ID	2768685
Full Name	molting defective
Synonyms	CG13620,CG31312,CG33343,CG34100,CG9469,DTS-3,Dmel\CG34100,L(3)3[DTS],Mld,l(3)3[DTS],l(3)DTS3
General protein information	Preferred Names molting defective Names CG34100 gene product from transcript CG34100-RD CG34100-PA CG34100-PC CG34100-PD CG34100-PE CG34100-PF defective dominant temperature-sensitive 3 lethal (3) DTS3 mld-PA mld-PC mld-PD mld-PE mld-PF molting
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 96A9-96A12\|3-84.5 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001043289.2	NP_001036754.1	molting defective, isoform A
NM_001260359.1	NP_001247288.1	molting defective, isoform F
NM_001043290.2	NP_001036755.2	molting defective, isoform C
NM_001260360.1	NP_001247289.1	molting defective, isoform D
NM_143016.2	NP_651273.2	molting defective, isoform E

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

spok expression is eliminated in larvae carrying mutations in molting defective (mld), a gene encoding a nuclear zinc finger protein that is required for production of ecdysone during Drosophila larval development.
Title: Spook and Spookier code for stage-specific components of the ecdysone biosynthetic pathway in Diptera.

molting defective encodes a nuclear zinc finger protein required for ecdysone biosynthesis
Title: molting defective is required for ecdysone biosynthesis.

The following mld gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mld cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa07277	NM_001043290.2 Latest version!	Drosophila melanogaster molting defective (mld), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OFa07277	NM_001043289.2 Latest version!	Drosophila melanogaster molting defective (mld), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OFa07278	NM_143016.2 Latest version!	Drosophila melanogaster molting defective (mld), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OFa08035	NM_001260360.1 Latest version!	Drosophila melanogaster molting defective (mld), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OFa08036	NM_001260359.1 Latest version!	Drosophila melanogaster molting defective (mld), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mld ( NM_001043289.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036754.1 Drosophila melanogaster molting defective (mld), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa07277
Clone ID Related Accession (Same CDS sequence)	NM_001043289.2 , NM_001043290.2
Accession Version	NM_001043289.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	molting defective, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On May 8, 2012 this sequence version replaced NM_001043289.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGCCA ACCGAAGAAG ACGCAGTGCA TCAGCTGCGA GTTCAATTGC AGCAGAAACA 
GCAACAGCAG CTACAGCAGC AACAGCAGAA GCGATATCAA CTGCACCCTC CAAAGAGCAA 
CTTTCTCCAC CGATTGCTGG TGGCAGTGCT TCAGCTGATG AGGATTTACT GGAAATGCCT 
CGCATTTGCC GATGCTGTTG CAAACGGGAT TTGAAATTAC TCGGCTTATT CGAGGCCAGC 
CAACCAACAC TGGCCAACAC AACAGTGGCA TCAGCATCAG CAGCAGCATC AGTGGCATCG 
AACAAATCAT CGGGAACATT AGCAAAAACA TGTGCGACAG AAGCAGAAAC CGAAACTCCA 
CAGACATTGT CCGCAAATCC AGTAAATCCC GGGACTAAAG CTACATCATC TCCATCAGAA 
GCAGCTACAA CGTTGTCCAC ATCCACACGC CCCGCGTCCG CCTCATTCTC ATCCACCTCC 
TTTTCCACAT CCACGTCTCC CATGAGGCGT CGCACCACTA ACACCACCGC TCCATCCGCT 
TCGCCCGCTC CACGTGGTCG TAGCAGCGAT AATGCCGTCG ACTACACCCT CAGCGGGGCC 
CACAGCAGCA TGGACATTGT CCTCGAGGAG ATGACCATTT GGATGCTCAA TATAAATCGC 
GACGATGGAC TGCCGCAAGA AATCTGCCGG CAGTGCATGG CCCAGTTTTT GATGGTGGCC 
AAGTTCCGGC GGAAATGTGT ACGGATGCAG CAGCGCCTGC AGGACTTTGC CCAGGAGATA 
TCCGACCGCC AGACGGCCAG ACAAGCGAAG CGTCAGCAAA AAGTCCAAAA GAAGACACAC 
GATAGGTGGC TGCAGTTGGA GCCAACCAGC CACGAGGAGC AGCAGCCCAT ACGTAAGCGC 
CGCATGGTAT CCGAATCGGA CGGAGATTCG ACAGCAGCAG CAGCAGCAGC AGAAGCAGCA 
ACAGCAACAG CAACAACACC AGCCAAACAG GTGCGAACGG ATTTCGAGAG TTTGCCACCG 
CCCAAACCAA GTCCTCAACA GCACCGAGTG CGCAAGTATC GAACCCTCTC CATTGACTGT 
GAAATGGAGC GTCCCGCCGC TTCCATCAAT GCCAGCAGCA TGCCCTGGAA GACGAAGGCT 
GCCCGCAAAG AACTTTCGGA GAATTGGGCG TCCAGTTGCT CGACGGCGGT TATGTCGCCC 
AATCGCCAAC GCAGCAGCAC CAGCAGCAGT GAGCACATGA GCGACTTGTC CGTCGGTCCA 
TGCTGTGGCT TCGAAAGCGA CAGCAATCCG TCCTCTACCT CCAACTCGGC GCGGAGAACG 
GTGATACTCG TTAAGCAGCA ATCGCCGGAG AAGACATCGC CCATGTATGA TTTAATGCCG 
CGCAGAGGAC GGCGGCCCAG GAAAGCACCG ATATACATGC CGGCCAAAAG ACAGAGGCGA 
TCGCTGGTTA GAAAGGAGCC GCTATACCGA AAAGCACAGC CAGTCAACGA AGAGAACAAA 
AAGTCTGATC AAAAGGATAT ATATCCGAAG GAATCAAGGA TGGAAGGCCT TGCACCGTCC 
TGTGATGAGG CCATCGACAG CACTTCCGAT GCGCCAATGG AAGTAGCCTC TAATGTGGTC 
CAAAAGAAGT GCGATGAAAT CTGTTTAAAT GGTTTGATAG ACAGTCGCAA CCAGTTAGGC 
GCCATGGAAA TGGAGCCGGT TAATGAAATG ATAGCGTCCA ATGGTGTAGA TAAAGAACTA 
ACCCTCAGCG TTCAAAATAA TAAGGAAACA ACATCCGAAG AGCAAATATT TGAAGCCACC 
AGCGTTATAA AAGAATCAGT TCAACCAGAA CAATCTGCTG AAGATATTCA GGTTAGAACG 
GAAATCGAGT CAATTGCGAA TGACATCAGG GCTGAGGCTG ATCTAGTGCA GTCCGAGGAA 
CTGACCTCAT CCACAGAGAC CAGCGAGTCG CAACCGAAAG CAGTTGAGCA GCTGGAATCT 
ATGGGAAAGC AGGAATCGTA TGCGTTACCA CAAAACATGG AGGCGCATGA ATCCGTCCAA 
CAAACGCAAA CTGTGGACGA ATCAGACTCT GCTGACCAGA GGGAGATCAT AGTGAGCATA 
CCGCTGGAGG TTCTGGATGA GAATCTGCAG CGTCGCCTTT GCGTGGATCC GGAAACTGAG 
GCGGCCAATC GTCAGTCCGC TGATCCCAAG GAGTTTGCCC TGCGTCCCGA TGACGTGAAC 
AACAACGAAA ACGATGAAAA TTTTTGCCAG AGTGCTACAG CAGATAGCTT CAATTCTGCT 
GTGGCACTCC GAACCAATAA AATTGAAGCA ACACCGCCTG GGGGAACGGA TTCAGATGAC 
ACGGACACGG ATGAGGTGCC GATAGTGCCA TCTGGGCCAA CGCCCATGGA TTTCATGGCC 
GAGTTTGCCA AGCACTGTGC GAACGGAATT GAACAGCTCA AGGTTACTAC ACCGGTGCCA 
TCACCGACTC CCTCGGATTT GGTACCCCTC AACGATCTGG AGGCCAAGCA GAAACTGGAG 
GTGGAGATGA ACGACGTGGA GACCACACTC AATGGCATCC TCAATGAGAT GCAGGACCAG 
CACATGTACA CACCAAACTG TGCCCACATT GATGAGTTCC TCACTCCAGC CGATTATGCC 
ACACTGACTG AGCAGGAGTC GTCCGTTTCG TCGCCACCAA ATCCTTTCGA GCAGAGTTCT 
GTTGCCGAAC CGAAGGACGA AAAGCCCAGT GCCACTGAGG ATCCATTTGA TAATAGCAAC 
TTTACCAATG AACTGATTGG TTTTCAAAAC GATATTCCCT GTTTCGAGAA CATTGAGACA 
CCAGAGGAGG GACAAAATTT GCATTTAGAA CTGACCCAGT TCTTAAGGGA TCTGCAGCCG 
TCACAATCCG ATCCGCCGGC AGGGGAGCAG GTGGAAAAAA TCGAAATGGA GACACAGCCA 
AGCACCGTCC AAATGGATGT CCAATCAAAC CTACCAGTCC AAGCTGCTGA TACACAGCCT 
CAAAATGAGT CCGCTAATCA TTCACCATTT GAATCTCCCA TATCATCACC TCAAGTTCAG 
TCTCAGATCG AATTACAAAT GGAACCTCAA ATCGAGACTC AAATGCAACC TCAAATTGAG 
ACTCGAATGC AACACGATGT GCTAAGCGTG CAATCGCCAC AAAACCATCA GCCTCAAGTA 
CAATCTCATG ACCAATCTCA GATGCAACAC CAAATCCCAC CGCAAATTGA GCCTCAAATT 
GAAGTCCAAA ACCAGGAGCA AAATACTTTA CAGCTCGTGC CGCAAGCACA GCATCAAGTG 
GTTCCACATG TACAACTGCA GTCCCAGTTG GTGCCTCACG TCCAGACGCA GGGCACGACG 
ACGACAGTCA CCTACGAACA GATTTACCAG CAGCACATTG TCCAGCAGAC GGTGCAGCAA 
TCCTCCAACA AGATGCAGGT CATCTCGCTG CCCTCGGCTG GCTCGGAAAC AACACGACAG 
TGGCAGACGC AGCAGTCGCA GCAACAGCAG CAAGTCCAGT GGTCATCGGT TGGCGGACTT 
GAGGCCATAA AGAGTGCACC CGTCGAGAGT GTTGCACCCA CGACCACAAC GTACTACATC 
AGTGCGAGCG ATCTTTATCC GCAGCAGACT GAAACATACA CGATGCTTCA ACCAGCATCC 
AATGAAAGCT ACGTCATTGA GCAGGTGAAT CATCAGCAGC AACATTGCCA GCAACAGATT 
CAGATGTCGC AACAGCAACA TCAACGCCAG CATCAGCAAC AGCAGGCGCA ACAGCAGCAG 
CAACCGATTA TGATACTGAT CCAGCAGCCA GAGATTCAGC AGCCAGTGGC CTCGGCCAGC 
AATCCGCAGC AGGCACCGCT GCACATCTAT AATGCTGCCA CCAGCAACGA TGTGCATCAA 
ATGCAGCACA CGCAACGCCA ACAGATACGG CAGCAGTATC AACACCAGCA GTATCAGCGG 
CTGCAACAGC CGCAACAAAC TCGCGTCGTA CAAAGCCATC CAGTATTGGG AATGCCGGGA 
GCCACTTCCA TATCAACGAA AGTGACCCCC ATTCCCGTCA CAGCGGCCAG GGGTCGGCCG 
CTCACGCTGA AGTGCCGCTT CTGCCATAAC GGGCCGCGAT TCTCCAGCAG CCTGGAGTAC 
AGCCGGCATA TCATCGATCT GCATCCGGCG GTGGCGCCCT TCAATTGTCC ACACTGTCCG 
ATGGCCTTTG CCGGCCGAAC GAAGCGATCG CAGCACATCC TCAGCCATCA TGTGGTGCAG 
CAGTACCAGT GCGGTCAGTG CTCACATGTG TTTCCCGCCC AGAAGGCACT GGACGTTCAC 
ATCCAGCGCT TTCATATGAC CCTGAAAACG GATCCATCGG GCGCTGTGAA GGTGGAGGAC 
GTTCAGCTGC AGATCACCAG CGAACGCAGG CCGCGGGGTA GGCCGTATAA GCCAAGGCTA 
CAGCTTCAAC AGCATCAACT GCAGCCGCAG CAGAAGTTAC AAGTGCAGCA GCATCAGCAG 
CAGCAGAAGG CACTTCAGCA ACTACCCCAA GCACAACACC ACCAACAGCA ACAGCAACCT 
CAACTTCAAC AAGAAGTGCT ACAGCAGGTG CAGCAACTGC AAGTGCAAGT GCAACCGCAG 
CAACACCAGC AACACCAACA AGTGTTGCAA GTGCAGCAAC CACAGCAACA ACCGTTAATG 
CAGCCACAGT CGCCGCATCC ATCAACAGTG GAAATGCAAG CAAGCCCAAC AACGCCACGA 
AAGATTCTTT GCTGTCCCGA TTGCGAAGAC TGCACTTCCG GCCACAGCCA TGCCAACGAA 
CAGTTCGAGG AGCTGCAGAC ACTGCAGGCG CCGCCAACGG TGTTGACTCC GCCATCGACC 
ATAGTCTCGG TACCATCGCC ACAGCCCATG GTGTACTCGC AGCACATAAC AATGCCGTCG 
CCGGAACAAT CCGAACCAGA TTCCACAACC ACGTTGCGAC AGTATCGCAA GCGAGGCGTG 
ATCGTTGGGC CACAGGGTCC GCTGCATTTG GCCACACCGG TGGCCTCGCC ATCGCCCTCG 
TCGTCACCCT CCTCATCGAC GGTGGATCAC ATACCGCCGG CATCTCCGGC TACGCCCGCA 
TCACCAGCTC CGCCTCCGTC GCCCGCCGTC GCGTCCACGG TGCAAGTGAG CGAGCTGCGA 
ACGAGTCACC ATTGTCTGTA CTGCGAGGAG CGATTCACCA ACGAAATTTC CCTGAAGAAG 
CACCATCAGC TGGCGCACGG AGCGCTGACA ACAATGCCAT ATGTGTGCAC AATCTGCAAG 
CGAGGGTATC GCATGCGTAC GGCGCTGCAT CGGCACATGG AGTCGCACGA TGTGGAGGGA 
CGGCCGTACG AGTGCAACAT TTGTCGCGTC CGGTTCCCGC GACCATCGCA ACTGACGCTG 
CACAAGATCA CGGTGCACCT GCTCTCCAAG CCGCACACCT GCGACGAGTG TGGCAAACAG 
TTTGGCACGG AGAGCGCCCT CAAGACGCAC ATTAAGTTCC ACGGAGCTCA CATGAAAACC 
CATTTGCCGC TGGGCGTATT CCGCAACGAG GATGCCGCAT CGAAGTCACC CAGCAACAAC 
AATAGCCACG CCAGCAGTGA CAAGCTGGAG AATCCAGCTA CGCCCATCGA GGAGACACCT 
TTGACGCCGA TGAGCAGTGG CGGCCACTTG TACCACAGCC CAGATGAATA CCCCAATTCG 
GTGGAGAGCT GCGCTGGCAA CAGTGTGGCT CTGGAATCCT ATGCCACAAC GCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036754.1
CDS	474..6230
Translation	MSANRRRRSASAASSIAAETATAATAATAEAISTAPSKEQLSPPIAGGSASADEDLLEMPRICRCCCKRDLKLLGLFEASQPTLANTTVASASAAASVASNKSSGTLAKTCATEAETETPQTLSANPVNPGTKATSSPSEAATTLSTSTRPASASFSSTSFSTSTSPMRRRTTNTTAPSASPAPRGRSSDNAVDYTLSGAHSSMDIVLEEMTIWMLNINRDDGLPQEICRQCMAQFLMVAKFRRKCVRMQQRLQDFAQEISDRQTARQAKRQQKVQKKTHDRWLQLEPTSHEEQQPIRKRRMVSESDGDSTAAAAAAEAATATATTPAKQVRTDFESLPPPKPSPQQHRVRKYRTLSIDCEMERPAASINASSMPWKTKAARKELSENWASSCSTAVMSPNRQRSSTSSSEHMSDLSVGPCCGFESDSNPSSTSNSARRTVILVKQQSPEKTSPMYDLMPRRGRRPRKAPIYMPAKRQRRSLVRKEPLYRKAQPVNEENKKSDQKDIYPKESRMEGLAPSCDEAIDSTSDAPMEVASNVVQKKCDEICLNGLIDSRNQLGAMEMEPVNEMIASNGVDKELTLSVQNNKETTSEEQIFEATSVIKESVQPEQSAEDIQVRTEIESIANDIRAEADLVQSEELTSSTETSESQPKAVEQLESMGKQESYALPQNMEAHESVQQTQTVDESDSADQREIIVSIPLEVLDENLQRRLCVDPETEAANRQSADPKEFALRPDDVNNNENDENFCQSATADSFNSAVALRTNKIEATPPGGTDSDDTDTDEVPIVPSGPTPMDFMAEFAKHCANGIEQLKVTTPVPSPTPSDLVPLNDLEAKQKLEVEMNDVETTLNGILNEMQDQHMYTPNCAHIDEFLTPADYATLTEQESSVSSPPNPFEQSSVAEPKDEKPSATEDPFDNSNFTNELIGFQNDIPCFENIETPEEGQNLHLELTQFLRDLQPSQSDPPAGEQVEKIEMETQPSTVQMDVQSNLPVQAADTQPQNESANHSPFESPISSPQVQSQIELQMEPQIETQMQPQIETRMQHDVLSVQSPQNHQPQVQSHDQSQMQHQIPPQIEPQIEVQNQEQNTLQLVPQAQHQVVPHVQLQSQLVPHVQTQGTTTTVTYEQIYQQHIVQQTVQQSSNKMQVISLPSAGSETTRQWQTQQSQQQQQVQWSSVGGLEAIKSAPVESVAPTTTTYYISASDLYPQQTETYTMLQPASNESYVIEQVNHQQQHCQQQIQMSQQQHQRQHQQQQAQQQQQPIMILIQQPEIQQPVASASNPQQAPLHIYNAATSNDVHQMQHTQRQQIRQQYQHQQYQRLQQPQQTRVVQSHPVLGMPGATSISTKVTPIPVTAARGRPLTLKCRFCHNGPRFSSSLEYSRHIIDLHPAVAPFNCPHCPMAFAGRTKRSQHILSHHVVQQYQCGQCSHVFPAQKALDVHIQRFHMTLKTDPSGAVKVEDVQLQITSERRPRGRPYKPRLQLQQHQLQPQQKLQVQQHQQQQKALQQLPQAQHHQQQQQPQLQQEVLQQVQQLQVQVQPQQHQQHQQVLQVQQPQQQPLMQPQSPHPSTVEMQASPTTPRKILCCPDCEDCTSGHSHANEQFEELQTLQAPPTVLTPPSTIVSVPSPQPMVYSQHITMPSPEQSEPDSTTTLRQYRKRGVIVGPQGPLHLATPVASPSPSSSPSSSTVDHIPPASPATPASPAPPPSPAVASTVQVSELRTSHHCLYCEERFTNEISLKKHHQLAHGALTTMPYVCTICKRGYRMRTALHRHMESHDVEGRPYECNICRVRFPRPSQLTLHKITVHLLSKPHTCDECGKQFGTESALKTHIKFHGAHMKTHLPLGVFRNEDAASKSPSNNNSHASSDKLENPATPIEETPLTPMSSGGHLYHSPDEYPNSVESCAGNSVALESYATTP

Target ORF information:

RefSeq Version	NM_001043289.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster molting defective (mld), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001043289.2

ORF Insert Sequence:

ATGAGTGCCA ACCGAAGAAG ACGCAGTGCA TCAGCTGCGA GTTCAATTGC AGCAGAAACA 
GCAACAGCAG CTACAGCAGC AACAGCAGAA GCGATATCAA CTGCACCCTC CAAAGAGCAA 
CTTTCTCCAC CGATTGCTGG TGGCAGTGCT TCAGCTGATG AGGATTTACT GGAAATGCCT 
CGCATTTGCC GATGCTGTTG CAAACGGGAT TTGAAATTAC TCGGCTTATT CGAGGCCAGC 
CAACCAACAC TGGCCAACAC AACAGTGGCA TCAGCATCAG CAGCAGCATC AGTGGCATCG 
AACAAATCAT CGGGAACATT AGCAAAAACA TGTGCGACAG AAGCAGAAAC CGAAACTCCA 
CAGACATTGT CCGCAAATCC AGTAAATCCC GGGACTAAAG CTACATCATC TCCATCAGAA 
GCAGCTACAA CGTTGTCCAC ATCCACACGC CCCGCGTCCG CCTCATTCTC ATCCACCTCC 
TTTTCCACAT CCACGTCTCC CATGAGGCGT CGCACCACTA ACACCACCGC TCCATCCGCT 
TCGCCCGCTC CACGTGGTCG TAGCAGCGAT AATGCCGTCG ACTACACCCT CAGCGGGGCC 
CACAGCAGCA TGGACATTGT CCTCGAGGAG ATGACCATTT GGATGCTCAA TATAAATCGC 
GACGATGGAC TGCCGCAAGA AATCTGCCGG CAGTGCATGG CCCAGTTTTT GATGGTGGCC 
AAGTTCCGGC GGAAATGTGT ACGGATGCAG CAGCGCCTGC AGGACTTTGC CCAGGAGATA 
TCCGACCGCC AGACGGCCAG ACAAGCGAAG CGTCAGCAAA AAGTCCAAAA GAAGACACAC 
GATAGGTGGC TGCAGTTGGA GCCAACCAGC CACGAGGAGC AGCAGCCCAT ACGTAAGCGC 
CGCATGGTAT CCGAATCGGA CGGAGATTCG ACAGCAGCAG CAGCAGCAGC AGAAGCAGCA 
ACAGCAACAG CAACAACACC AGCCAAACAG GTGCGAACGG ATTTCGAGAG TTTGCCACCG 
CCCAAACCAA GTCCTCAACA GCACCGAGTG CGCAAGTATC GAACCCTCTC CATTGACTGT 
GAAATGGAGC GTCCCGCCGC TTCCATCAAT GCCAGCAGCA TGCCCTGGAA GACGAAGGCT 
GCCCGCAAAG AACTTTCGGA GAATTGGGCG TCCAGTTGCT CGACGGCGGT TATGTCGCCC 
AATCGCCAAC GCAGCAGCAC CAGCAGCAGT GAGCACATGA GCGACTTGTC CGTCGGTCCA 
TGCTGTGGCT TCGAAAGCGA CAGCAATCCG TCCTCTACCT CCAACTCGGC GCGGAGAACG 
GTGATACTCG TTAAGCAGCA ATCGCCGGAG AAGACATCGC CCATGTATGA TTTAATGCCG 
CGCAGAGGAC GGCGGCCCAG GAAAGCACCG ATATACATGC CGGCCAAAAG ACAGAGGCGA 
TCGCTGGTTA GAAAGGAGCC GCTATACCGA AAAGCACAGC CAGTCAACGA AGAGAACAAA 
AAGTCTGATC AAAAGGATAT ATATCCGAAG GAATCAAGGA TGGAAGGCCT TGCACCGTCC 
TGTGATGAGG CCATCGACAG CACTTCCGAT GCGCCAATGG AAGTAGCCTC TAATGTGGTC 
CAAAAGAAGT GCGATGAAAT CTGTTTAAAT GGTTTGATAG ACAGTCGCAA CCAGTTAGGC 
GCCATGGAAA TGGAGCCGGT TAATGAAATG ATAGCGTCCA ATGGTGTAGA TAAAGAACTA 
ACCCTCAGCG TTCAAAATAA TAAGGAAACA ACATCCGAAG AGCAAATATT TGAAGCCACC 
AGCGTTATAA AAGAATCAGT TCAACCAGAA CAATCTGCTG AAGATATTCA GGTTAGAACG 
GAAATCGAGT CAATTGCGAA TGACATCAGG GCTGAGGCTG ATCTAGTGCA GTCCGAGGAA 
CTGACCTCAT CCACAGAGAC CAGCGAGTCG CAACCGAAAG CAGTTGAGCA GCTGGAATCT 
ATGGGAAAGC AGGAATCGTA TGCGTTACCA CAAAACATGG AGGCGCATGA ATCCGTCCAA 
CAAACGCAAA CTGTGGACGA ATCAGACTCT GCTGACCAGA GGGAGATCAT AGTGAGCATA 
CCGCTGGAGG TTCTGGATGA GAATCTGCAG CGTCGCCTTT GCGTGGATCC GGAAACTGAG 
GCGGCCAATC GTCAGTCCGC TGATCCCAAG GAGTTTGCCC TGCGTCCCGA TGACGTGAAC 
AACAACGAAA ACGATGAAAA TTTTTGCCAG AGTGCTACAG CAGATAGCTT CAATTCTGCT 
GTGGCACTCC GAACCAATAA AATTGAAGCA ACACCGCCTG GGGGAACGGA TTCAGATGAC 
ACGGACACGG ATGAGGTGCC GATAGTGCCA TCTGGGCCAA CGCCCATGGA TTTCATGGCC 
GAGTTTGCCA AGCACTGTGC GAACGGAATT GAACAGCTCA AGGTTACTAC ACCGGTGCCA 
TCACCGACTC CCTCGGATTT GGTACCCCTC AACGATCTGG AGGCCAAGCA GAAACTGGAG 
GTGGAGATGA ACGACGTGGA GACCACACTC AATGGCATCC TCAATGAGAT GCAGGACCAG 
CACATGTACA CACCAAACTG TGCCCACATT GATGAGTTCC TCACTCCAGC CGATTATGCC 
ACACTGACTG AGCAGGAGTC GTCCGTTTCG TCGCCACCAA ATCCTTTCGA GCAGAGTTCT 
GTTGCCGAAC CGAAGGACGA AAAGCCCAGT GCCACTGAGG ATCCATTTGA TAATAGCAAC 
TTTACCAATG AACTGATTGG TTTTCAAAAC GATATTCCCT GTTTCGAGAA CATTGAGACA 
CCAGAGGAGG GACAAAATTT GCATTTAGAA CTGACCCAGT TCTTAAGGGA TCTGCAGCCG 
TCACAATCCG ATCCGCCGGC AGGGGAGCAG GTGGAAAAAA TCGAAATGGA GACACAGCCA 
AGCACCGTCC AAATGGATGT CCAATCAAAC CTACCAGTCC AAGCTGCTGA TACACAGCCT 
CAAAATGAGT CCGCTAATCA TTCACCATTT GAATCTCCCA TATCATCACC TCAAGTTCAG 
TCTCAGATCG AATTACAAAT GGAACCTCAA ATCGAGACTC AAATGCAACC TCAAATTGAG 
ACTCGAATGC AACACGATGT GCTAAGCGTG CAATCGCCAC AAAACCATCA GCCTCAAGTA 
CAATCTCATG ACCAATCTCA GATGCAACAC CAAATCCCAC CGCAAATTGA GCCTCAAATT 
GAAGTCCAAA ACCAGGAGCA AAATACTTTA CAGCTCGTGC CGCAAGCACA GCATCAAGTG 
GTTCCACATG TACAACTGCA GTCCCAGTTG GTGCCTCACG TCCAGACGCA GGGCACGACG 
ACGACAGTCA CCTACGAACA GATTTACCAG CAGCACATTG TCCAGCAGAC GGTGCAGCAA 
TCCTCCAACA AGATGCAGGT CATCTCGCTG CCCTCGGCTG GCTCGGAAAC AACACGACAG 
TGGCAGACGC AGCAGTCGCA GCAACAGCAG CAAGTCCAGT GGTCATCGGT TGGCGGACTT 
GAGGCCATAA AGAGTGCACC CGTCGAGAGT GTTGCACCCA CGACCACAAC GTACTACATC 
AGTGCGAGCG ATCTTTATCC GCAGCAGACT GAAACATACA CGATGCTTCA ACCAGCATCC 
AATGAAAGCT ACGTCATTGA GCAGGTGAAT CATCAGCAGC AACATTGCCA GCAACAGATT 
CAGATGTCGC AACAGCAACA TCAACGCCAG CATCAGCAAC AGCAGGCGCA ACAGCAGCAG 
CAACCGATTA TGATACTGAT CCAGCAGCCA GAGATTCAGC AGCCAGTGGC CTCGGCCAGC 
AATCCGCAGC AGGCACCGCT GCACATCTAT AATGCTGCCA CCAGCAACGA TGTGCATCAA 
ATGCAGCACA CGCAACGCCA ACAGATACGG CAGCAGTATC AACACCAGCA GTATCAGCGG 
CTGCAACAGC CGCAACAAAC TCGCGTCGTA CAAAGCCATC CAGTATTGGG AATGCCGGGA 
GCCACTTCCA TATCAACGAA AGTGACCCCC ATTCCCGTCA CAGCGGCCAG GGGTCGGCCG 
CTCACGCTGA AGTGCCGCTT CTGCCATAAC GGGCCGCGAT TCTCCAGCAG CCTGGAGTAC 
AGCCGGCATA TCATCGATCT GCATCCGGCG GTGGCGCCCT TCAATTGTCC ACACTGTCCG 
ATGGCCTTTG CCGGCCGAAC GAAGCGATCG CAGCACATCC TCAGCCATCA TGTGGTGCAG 
CAGTACCAGT GCGGTCAGTG CTCACATGTG TTTCCCGCCC AGAAGGCACT GGACGTTCAC 
ATCCAGCGCT TTCATATGAC CCTGAAAACG GATCCATCGG GCGCTGTGAA GGTGGAGGAC 
GTTCAGCTGC AGATCACCAG CGAACGCAGG CCGCGGGGTA GGCCGTATAA GCCAAGGCTA 
CAGCTTCAAC AGCATCAACT GCAGCCGCAG CAGAAGTTAC AAGTGCAGCA GCATCAGCAG 
CAGCAGAAGG CACTTCAGCA ACTACCCCAA GCACAACACC ACCAACAGCA ACAGCAACCT 
CAACTTCAAC AAGAAGTGCT ACAGCAGGTG CAGCAACTGC AAGTGCAAGT GCAACCGCAG 
CAACACCAGC AACACCAACA AGTGTTGCAA GTGCAGCAAC CACAGCAACA ACCGTTAATG 
CAGCCACAGT CGCCGCATCC ATCAACAGTG GAAATGCAAG CAAGCCCAAC AACGCCACGA 
AAGATTCTTT GCTGTCCCGA TTGCGAAGAC TGCACTTCCG GCCACAGCCA TGCCAACGAA 
CAGTTCGAGG AGCTGCAGAC ACTGCAGGCG CCGCCAACGG TGTTGACTCC GCCATCGACC 
ATAGTCTCGG TACCATCGCC ACAGCCCATG GTGTACTCGC AGCACATAAC AATGCCGTCG 
CCGGAACAAT CCGAACCAGA TTCCACAACC ACGTTGCGAC AGTATCGCAA GCGAGGCGTG 
ATCGTTGGGC CACAGGGTCC GCTGCATTTG GCCACACCGG TGGCCTCGCC ATCGCCCTCG 
TCGTCACCCT CCTCATCGAC GGTGGATCAC ATACCGCCGG CATCTCCGGC TACGCCCGCA 
TCACCAGCTC CGCCTCCGTC GCCCGCCGTC GCGTCCACGG TGCAAGTGAG CGAGCTGCGA 
ACGAGTCACC ATTGTCTGTA CTGCGAGGAG CGATTCACCA ACGAAATTTC CCTGAAGAAG 
CACCATCAGC TGGCGCACGG AGCGCTGACA ACAATGCCAT ATGTGTGCAC AATCTGCAAG 
CGAGGGTATC GCATGCGTAC GGCGCTGCAT CGGCACATGG AGTCGCACGA TGTGGAGGGA 
CGGCCGTACG AGTGCAACAT TTGTCGCGTC CGGTTCCCGC GACCATCGCA ACTGACGCTG 
CACAAGATCA CGGTGCACCT GCTCTCCAAG CCGCACACCT GCGACGAGTG TGGCAAACAG 
TTTGGCACGG AGAGCGCCCT CAAGACGCAC ATTAAGTTCC ACGGAGCTCA CATGAAAACC 
CATTTGCCGC TGGGCGTATT CCGCAACGAG GATGCCGCAT CGAAGTCACC CAGCAACAAC 
AATAGCCACG CCAGCAGTGA CAAGCTGGAG AATCCAGCTA CGCCCATCGA GGAGACACCT 
TTGACGCCGA TGAGCAGTGG CGGCCACTTG TACCACAGCC CAGATGAATA CCCCAATTCG 
GTGGAGAGCT GCGCTGGCAA CAGTGTGGCT CTGGAATCCT ATGCCACAAC GCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mld ( NM_001043290.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001036755.2 Drosophila melanogaster molting defective (mld), transcript variant C, mRNA.

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CloneID	OFa07277
Clone ID Related Accession (Same CDS sequence)	NM_001043289.2 , NM_001043290.2
Accession Version	NM_001043290.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	molting defective, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On May 8, 2012 this sequence version replaced NM_001043290.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGCCA ACCGAAGAAG ACGCAGTGCA TCAGCTGCGA GTTCAATTGC AGCAGAAACA 
GCAACAGCAG CTACAGCAGC AACAGCAGAA GCGATATCAA CTGCACCCTC CAAAGAGCAA 
CTTTCTCCAC CGATTGCTGG TGGCAGTGCT TCAGCTGATG AGGATTTACT GGAAATGCCT 
CGCATTTGCC GATGCTGTTG CAAACGGGAT TTGAAATTAC TCGGCTTATT CGAGGCCAGC 
CAACCAACAC TGGCCAACAC AACAGTGGCA TCAGCATCAG CAGCAGCATC AGTGGCATCG 
AACAAATCAT CGGGAACATT AGCAAAAACA TGTGCGACAG AAGCAGAAAC CGAAACTCCA 
CAGACATTGT CCGCAAATCC AGTAAATCCC GGGACTAAAG CTACATCATC TCCATCAGAA 
GCAGCTACAA CGTTGTCCAC ATCCACACGC CCCGCGTCCG CCTCATTCTC ATCCACCTCC 
TTTTCCACAT CCACGTCTCC CATGAGGCGT CGCACCACTA ACACCACCGC TCCATCCGCT 
TCGCCCGCTC CACGTGGTCG TAGCAGCGAT AATGCCGTCG ACTACACCCT CAGCGGGGCC 
CACAGCAGCA TGGACATTGT CCTCGAGGAG ATGACCATTT GGATGCTCAA TATAAATCGC 
GACGATGGAC TGCCGCAAGA AATCTGCCGG CAGTGCATGG CCCAGTTTTT GATGGTGGCC 
AAGTTCCGGC GGAAATGTGT ACGGATGCAG CAGCGCCTGC AGGACTTTGC CCAGGAGATA 
TCCGACCGCC AGACGGCCAG ACAAGCGAAG CGTCAGCAAA AAGTCCAAAA GAAGACACAC 
GATAGGTGGC TGCAGTTGGA GCCAACCAGC CACGAGGAGC AGCAGCCCAT ACGTAAGCGC 
CGCATGGTAT CCGAATCGGA CGGAGATTCG ACAGCAGCAG CAGCAGCAGC AGAAGCAGCA 
ACAGCAACAG CAACAACACC AGCCAAACAG GTGCGAACGG ATTTCGAGAG TTTGCCACCG 
CCCAAACCAA GTCCTCAACA GCACCGAGTG CGCAAGTATC GAACCCTCTC CATTGACTGT 
GAAATGGAGC GTCCCGCCGC TTCCATCAAT GCCAGCAGCA TGCCCTGGAA GACGAAGGCT 
GCCCGCAAAG AACTTTCGGA GAATTGGGCG TCCAGTTGCT CGACGGCGGT TATGTCGCCC 
AATCGCCAAC GCAGCAGCAC CAGCAGCAGT GAGCACATGA GCGACTTGTC CGTCGGTCCA 
TGCTGTGGCT TCGAAAGCGA CAGCAATCCG TCCTCTACCT CCAACTCGGC GCGGAGAACG 
GTGATACTCG TTAAGCAGCA ATCGCCGGAG AAGACATCGC CCATGTATGA TTTAATGCCG 
CGCAGAGGAC GGCGGCCCAG GAAAGCACCG ATATACATGC CGGCCAAAAG ACAGAGGCGA 
TCGCTGGTTA GAAAGGAGCC GCTATACCGA AAAGCACAGC CAGTCAACGA AGAGAACAAA 
AAGTCTGATC AAAAGGATAT ATATCCGAAG GAATCAAGGA TGGAAGGCCT TGCACCGTCC 
TGTGATGAGG CCATCGACAG CACTTCCGAT GCGCCAATGG AAGTAGCCTC TAATGTGGTC 
CAAAAGAAGT GCGATGAAAT CTGTTTAAAT GGTTTGATAG ACAGTCGCAA CCAGTTAGGC 
GCCATGGAAA TGGAGCCGGT TAATGAAATG ATAGCGTCCA ATGGTGTAGA TAAAGAACTA 
ACCCTCAGCG TTCAAAATAA TAAGGAAACA ACATCCGAAG AGCAAATATT TGAAGCCACC 
AGCGTTATAA AAGAATCAGT TCAACCAGAA CAATCTGCTG AAGATATTCA GGTTAGAACG 
GAAATCGAGT CAATTGCGAA TGACATCAGG GCTGAGGCTG ATCTAGTGCA GTCCGAGGAA 
CTGACCTCAT CCACAGAGAC CAGCGAGTCG CAACCGAAAG CAGTTGAGCA GCTGGAATCT 
ATGGGAAAGC AGGAATCGTA TGCGTTACCA CAAAACATGG AGGCGCATGA ATCCGTCCAA 
CAAACGCAAA CTGTGGACGA ATCAGACTCT GCTGACCAGA GGGAGATCAT AGTGAGCATA 
CCGCTGGAGG TTCTGGATGA GAATCTGCAG CGTCGCCTTT GCGTGGATCC GGAAACTGAG 
GCGGCCAATC GTCAGTCCGC TGATCCCAAG GAGTTTGCCC TGCGTCCCGA TGACGTGAAC 
AACAACGAAA ACGATGAAAA TTTTTGCCAG AGTGCTACAG CAGATAGCTT CAATTCTGCT 
GTGGCACTCC GAACCAATAA AATTGAAGCA ACACCGCCTG GGGGAACGGA TTCAGATGAC 
ACGGACACGG ATGAGGTGCC GATAGTGCCA TCTGGGCCAA CGCCCATGGA TTTCATGGCC 
GAGTTTGCCA AGCACTGTGC GAACGGAATT GAACAGCTCA AGGTTACTAC ACCGGTGCCA 
TCACCGACTC CCTCGGATTT GGTACCCCTC AACGATCTGG AGGCCAAGCA GAAACTGGAG 
GTGGAGATGA ACGACGTGGA GACCACACTC AATGGCATCC TCAATGAGAT GCAGGACCAG 
CACATGTACA CACCAAACTG TGCCCACATT GATGAGTTCC TCACTCCAGC CGATTATGCC 
ACACTGACTG AGCAGGAGTC GTCCGTTTCG TCGCCACCAA ATCCTTTCGA GCAGAGTTCT 
GTTGCCGAAC CGAAGGACGA AAAGCCCAGT GCCACTGAGG ATCCATTTGA TAATAGCAAC 
TTTACCAATG AACTGATTGG TTTTCAAAAC GATATTCCCT GTTTCGAGAA CATTGAGACA 
CCAGAGGAGG GACAAAATTT GCATTTAGAA CTGACCCAGT TCTTAAGGGA TCTGCAGCCG 
TCACAATCCG ATCCGCCGGC AGGGGAGCAG GTGGAAAAAA TCGAAATGGA GACACAGCCA 
AGCACCGTCC AAATGGATGT CCAATCAAAC CTACCAGTCC AAGCTGCTGA TACACAGCCT 
CAAAATGAGT CCGCTAATCA TTCACCATTT GAATCTCCCA TATCATCACC TCAAGTTCAG 
TCTCAGATCG AATTACAAAT GGAACCTCAA ATCGAGACTC AAATGCAACC TCAAATTGAG 
ACTCGAATGC AACACGATGT GCTAAGCGTG CAATCGCCAC AAAACCATCA GCCTCAAGTA 
CAATCTCATG ACCAATCTCA GATGCAACAC CAAATCCCAC CGCAAATTGA GCCTCAAATT 
GAAGTCCAAA ACCAGGAGCA AAATACTTTA CAGCTCGTGC CGCAAGCACA GCATCAAGTG 
GTTCCACATG TACAACTGCA GTCCCAGTTG GTGCCTCACG TCCAGACGCA GGGCACGACG 
ACGACAGTCA CCTACGAACA GATTTACCAG CAGCACATTG TCCAGCAGAC GGTGCAGCAA 
TCCTCCAACA AGATGCAGGT CATCTCGCTG CCCTCGGCTG GCTCGGAAAC AACACGACAG 
TGGCAGACGC AGCAGTCGCA GCAACAGCAG CAAGTCCAGT GGTCATCGGT TGGCGGACTT 
GAGGCCATAA AGAGTGCACC CGTCGAGAGT GTTGCACCCA CGACCACAAC GTACTACATC 
AGTGCGAGCG ATCTTTATCC GCAGCAGACT GAAACATACA CGATGCTTCA ACCAGCATCC 
AATGAAAGCT ACGTCATTGA GCAGGTGAAT CATCAGCAGC AACATTGCCA GCAACAGATT 
CAGATGTCGC AACAGCAACA TCAACGCCAG CATCAGCAAC AGCAGGCGCA ACAGCAGCAG 
CAACCGATTA TGATACTGAT CCAGCAGCCA GAGATTCAGC AGCCAGTGGC CTCGGCCAGC 
AATCCGCAGC AGGCACCGCT GCACATCTAT AATGCTGCCA CCAGCAACGA TGTGCATCAA 
ATGCAGCACA CGCAACGCCA ACAGATACGG CAGCAGTATC AACACCAGCA GTATCAGCGG 
CTGCAACAGC CGCAACAAAC TCGCGTCGTA CAAAGCCATC CAGTATTGGG AATGCCGGGA 
GCCACTTCCA TATCAACGAA AGTGACCCCC ATTCCCGTCA CAGCGGCCAG GGGTCGGCCG 
CTCACGCTGA AGTGCCGCTT CTGCCATAAC GGGCCGCGAT TCTCCAGCAG CCTGGAGTAC 
AGCCGGCATA TCATCGATCT GCATCCGGCG GTGGCGCCCT TCAATTGTCC ACACTGTCCG 
ATGGCCTTTG CCGGCCGAAC GAAGCGATCG CAGCACATCC TCAGCCATCA TGTGGTGCAG 
CAGTACCAGT GCGGTCAGTG CTCACATGTG TTTCCCGCCC AGAAGGCACT GGACGTTCAC 
ATCCAGCGCT TTCATATGAC CCTGAAAACG GATCCATCGG GCGCTGTGAA GGTGGAGGAC 
GTTCAGCTGC AGATCACCAG CGAACGCAGG CCGCGGGGTA GGCCGTATAA GCCAAGGCTA 
CAGCTTCAAC AGCATCAACT GCAGCCGCAG CAGAAGTTAC AAGTGCAGCA GCATCAGCAG 
CAGCAGAAGG CACTTCAGCA ACTACCCCAA GCACAACACC ACCAACAGCA ACAGCAACCT 
CAACTTCAAC AAGAAGTGCT ACAGCAGGTG CAGCAACTGC AAGTGCAAGT GCAACCGCAG 
CAACACCAGC AACACCAACA AGTGTTGCAA GTGCAGCAAC CACAGCAACA ACCGTTAATG 
CAGCCACAGT CGCCGCATCC ATCAACAGTG GAAATGCAAG CAAGCCCAAC AACGCCACGA 
AAGATTCTTT GCTGTCCCGA TTGCGAAGAC TGCACTTCCG GCCACAGCCA TGCCAACGAA 
CAGTTCGAGG AGCTGCAGAC ACTGCAGGCG CCGCCAACGG TGTTGACTCC GCCATCGACC 
ATAGTCTCGG TACCATCGCC ACAGCCCATG GTGTACTCGC AGCACATAAC AATGCCGTCG 
CCGGAACAAT CCGAACCAGA TTCCACAACC ACGTTGCGAC AGTATCGCAA GCGAGGCGTG 
ATCGTTGGGC CACAGGGTCC GCTGCATTTG GCCACACCGG TGGCCTCGCC ATCGCCCTCG 
TCGTCACCCT CCTCATCGAC GGTGGATCAC ATACCGCCGG CATCTCCGGC TACGCCCGCA 
TCACCAGCTC CGCCTCCGTC GCCCGCCGTC GCGTCCACGG TGCAAGTGAG CGAGCTGCGA 
ACGAGTCACC ATTGTCTGTA CTGCGAGGAG CGATTCACCA ACGAAATTTC CCTGAAGAAG 
CACCATCAGC TGGCGCACGG AGCGCTGACA ACAATGCCAT ATGTGTGCAC AATCTGCAAG 
CGAGGGTATC GCATGCGTAC GGCGCTGCAT CGGCACATGG AGTCGCACGA TGTGGAGGGA 
CGGCCGTACG AGTGCAACAT TTGTCGCGTC CGGTTCCCGC GACCATCGCA ACTGACGCTG 
CACAAGATCA CGGTGCACCT GCTCTCCAAG CCGCACACCT GCGACGAGTG TGGCAAACAG 
TTTGGCACGG AGAGCGCCCT CAAGACGCAC ATTAAGTTCC ACGGAGCTCA CATGAAAACC 
CATTTGCCGC TGGGCGTATT CCGCAACGAG GATGCCGCAT CGAAGTCACC CAGCAACAAC 
AATAGCCACG CCAGCAGTGA CAAGCTGGAG AATCCAGCTA CGCCCATCGA GGAGACACCT 
TTGACGCCGA TGAGCAGTGG CGGCCACTTG TACCACAGCC CAGATGAATA CCCCAATTCG 
GTGGAGAGCT GCGCTGGCAA CAGTGTGGCT CTGGAATCCT ATGCCACAAC GCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036755.2
CDS	471..6227
Translation	MSANRRRRSASAASSIAAETATAATAATAEAISTAPSKEQLSPPIAGGSASADEDLLEMPRICRCCCKRDLKLLGLFEASQPTLANTTVASASAAASVASNKSSGTLAKTCATEAETETPQTLSANPVNPGTKATSSPSEAATTLSTSTRPASASFSSTSFSTSTSPMRRRTTNTTAPSASPAPRGRSSDNAVDYTLSGAHSSMDIVLEEMTIWMLNINRDDGLPQEICRQCMAQFLMVAKFRRKCVRMQQRLQDFAQEISDRQTARQAKRQQKVQKKTHDRWLQLEPTSHEEQQPIRKRRMVSESDGDSTAAAAAAEAATATATTPAKQVRTDFESLPPPKPSPQQHRVRKYRTLSIDCEMERPAASINASSMPWKTKAARKELSENWASSCSTAVMSPNRQRSSTSSSEHMSDLSVGPCCGFESDSNPSSTSNSARRTVILVKQQSPEKTSPMYDLMPRRGRRPRKAPIYMPAKRQRRSLVRKEPLYRKAQPVNEENKKSDQKDIYPKESRMEGLAPSCDEAIDSTSDAPMEVASNVVQKKCDEICLNGLIDSRNQLGAMEMEPVNEMIASNGVDKELTLSVQNNKETTSEEQIFEATSVIKESVQPEQSAEDIQVRTEIESIANDIRAEADLVQSEELTSSTETSESQPKAVEQLESMGKQESYALPQNMEAHESVQQTQTVDESDSADQREIIVSIPLEVLDENLQRRLCVDPETEAANRQSADPKEFALRPDDVNNNENDENFCQSATADSFNSAVALRTNKIEATPPGGTDSDDTDTDEVPIVPSGPTPMDFMAEFAKHCANGIEQLKVTTPVPSPTPSDLVPLNDLEAKQKLEVEMNDVETTLNGILNEMQDQHMYTPNCAHIDEFLTPADYATLTEQESSVSSPPNPFEQSSVAEPKDEKPSATEDPFDNSNFTNELIGFQNDIPCFENIETPEEGQNLHLELTQFLRDLQPSQSDPPAGEQVEKIEMETQPSTVQMDVQSNLPVQAADTQPQNESANHSPFESPISSPQVQSQIELQMEPQIETQMQPQIETRMQHDVLSVQSPQNHQPQVQSHDQSQMQHQIPPQIEPQIEVQNQEQNTLQLVPQAQHQVVPHVQLQSQLVPHVQTQGTTTTVTYEQIYQQHIVQQTVQQSSNKMQVISLPSAGSETTRQWQTQQSQQQQQVQWSSVGGLEAIKSAPVESVAPTTTTYYISASDLYPQQTETYTMLQPASNESYVIEQVNHQQQHCQQQIQMSQQQHQRQHQQQQAQQQQQPIMILIQQPEIQQPVASASNPQQAPLHIYNAATSNDVHQMQHTQRQQIRQQYQHQQYQRLQQPQQTRVVQSHPVLGMPGATSISTKVTPIPVTAARGRPLTLKCRFCHNGPRFSSSLEYSRHIIDLHPAVAPFNCPHCPMAFAGRTKRSQHILSHHVVQQYQCGQCSHVFPAQKALDVHIQRFHMTLKTDPSGAVKVEDVQLQITSERRPRGRPYKPRLQLQQHQLQPQQKLQVQQHQQQQKALQQLPQAQHHQQQQQPQLQQEVLQQVQQLQVQVQPQQHQQHQQVLQVQQPQQQPLMQPQSPHPSTVEMQASPTTPRKILCCPDCEDCTSGHSHANEQFEELQTLQAPPTVLTPPSTIVSVPSPQPMVYSQHITMPSPEQSEPDSTTTLRQYRKRGVIVGPQGPLHLATPVASPSPSSSPSSSTVDHIPPASPATPASPAPPPSPAVASTVQVSELRTSHHCLYCEERFTNEISLKKHHQLAHGALTTMPYVCTICKRGYRMRTALHRHMESHDVEGRPYECNICRVRFPRPSQLTLHKITVHLLSKPHTCDECGKQFGTESALKTHIKFHGAHMKTHLPLGVFRNEDAASKSPSNNNSHASSDKLENPATPIEETPLTPMSSGGHLYHSPDEYPNSVESCAGNSVALESYATTP

Target ORF information:

RefSeq Version	NM_001043290.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster molting defective (mld), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001043290.2

ORF Insert Sequence:

ATGAGTGCCA ACCGAAGAAG ACGCAGTGCA TCAGCTGCGA GTTCAATTGC AGCAGAAACA 
GCAACAGCAG CTACAGCAGC AACAGCAGAA GCGATATCAA CTGCACCCTC CAAAGAGCAA 
CTTTCTCCAC CGATTGCTGG TGGCAGTGCT TCAGCTGATG AGGATTTACT GGAAATGCCT 
CGCATTTGCC GATGCTGTTG CAAACGGGAT TTGAAATTAC TCGGCTTATT CGAGGCCAGC 
CAACCAACAC TGGCCAACAC AACAGTGGCA TCAGCATCAG CAGCAGCATC AGTGGCATCG 
AACAAATCAT CGGGAACATT AGCAAAAACA TGTGCGACAG AAGCAGAAAC CGAAACTCCA 
CAGACATTGT CCGCAAATCC AGTAAATCCC GGGACTAAAG CTACATCATC TCCATCAGAA 
GCAGCTACAA CGTTGTCCAC ATCCACACGC CCCGCGTCCG CCTCATTCTC ATCCACCTCC 
TTTTCCACAT CCACGTCTCC CATGAGGCGT CGCACCACTA ACACCACCGC TCCATCCGCT 
TCGCCCGCTC CACGTGGTCG TAGCAGCGAT AATGCCGTCG ACTACACCCT CAGCGGGGCC 
CACAGCAGCA TGGACATTGT CCTCGAGGAG ATGACCATTT GGATGCTCAA TATAAATCGC 
GACGATGGAC TGCCGCAAGA AATCTGCCGG CAGTGCATGG CCCAGTTTTT GATGGTGGCC 
AAGTTCCGGC GGAAATGTGT ACGGATGCAG CAGCGCCTGC AGGACTTTGC CCAGGAGATA 
TCCGACCGCC AGACGGCCAG ACAAGCGAAG CGTCAGCAAA AAGTCCAAAA GAAGACACAC 
GATAGGTGGC TGCAGTTGGA GCCAACCAGC CACGAGGAGC AGCAGCCCAT ACGTAAGCGC 
CGCATGGTAT CCGAATCGGA CGGAGATTCG ACAGCAGCAG CAGCAGCAGC AGAAGCAGCA 
ACAGCAACAG CAACAACACC AGCCAAACAG GTGCGAACGG ATTTCGAGAG TTTGCCACCG 
CCCAAACCAA GTCCTCAACA GCACCGAGTG CGCAAGTATC GAACCCTCTC CATTGACTGT 
GAAATGGAGC GTCCCGCCGC TTCCATCAAT GCCAGCAGCA TGCCCTGGAA GACGAAGGCT 
GCCCGCAAAG AACTTTCGGA GAATTGGGCG TCCAGTTGCT CGACGGCGGT TATGTCGCCC 
AATCGCCAAC GCAGCAGCAC CAGCAGCAGT GAGCACATGA GCGACTTGTC CGTCGGTCCA 
TGCTGTGGCT TCGAAAGCGA CAGCAATCCG TCCTCTACCT CCAACTCGGC GCGGAGAACG 
GTGATACTCG TTAAGCAGCA ATCGCCGGAG AAGACATCGC CCATGTATGA TTTAATGCCG 
CGCAGAGGAC GGCGGCCCAG GAAAGCACCG ATATACATGC CGGCCAAAAG ACAGAGGCGA 
TCGCTGGTTA GAAAGGAGCC GCTATACCGA AAAGCACAGC CAGTCAACGA AGAGAACAAA 
AAGTCTGATC AAAAGGATAT ATATCCGAAG GAATCAAGGA TGGAAGGCCT TGCACCGTCC 
TGTGATGAGG CCATCGACAG CACTTCCGAT GCGCCAATGG AAGTAGCCTC TAATGTGGTC 
CAAAAGAAGT GCGATGAAAT CTGTTTAAAT GGTTTGATAG ACAGTCGCAA CCAGTTAGGC 
GCCATGGAAA TGGAGCCGGT TAATGAAATG ATAGCGTCCA ATGGTGTAGA TAAAGAACTA 
ACCCTCAGCG TTCAAAATAA TAAGGAAACA ACATCCGAAG AGCAAATATT TGAAGCCACC 
AGCGTTATAA AAGAATCAGT TCAACCAGAA CAATCTGCTG AAGATATTCA GGTTAGAACG 
GAAATCGAGT CAATTGCGAA TGACATCAGG GCTGAGGCTG ATCTAGTGCA GTCCGAGGAA 
CTGACCTCAT CCACAGAGAC CAGCGAGTCG CAACCGAAAG CAGTTGAGCA GCTGGAATCT 
ATGGGAAAGC AGGAATCGTA TGCGTTACCA CAAAACATGG AGGCGCATGA ATCCGTCCAA 
CAAACGCAAA CTGTGGACGA ATCAGACTCT GCTGACCAGA GGGAGATCAT AGTGAGCATA 
CCGCTGGAGG TTCTGGATGA GAATCTGCAG CGTCGCCTTT GCGTGGATCC GGAAACTGAG 
GCGGCCAATC GTCAGTCCGC TGATCCCAAG GAGTTTGCCC TGCGTCCCGA TGACGTGAAC 
AACAACGAAA ACGATGAAAA TTTTTGCCAG AGTGCTACAG CAGATAGCTT CAATTCTGCT 
GTGGCACTCC GAACCAATAA AATTGAAGCA ACACCGCCTG GGGGAACGGA TTCAGATGAC 
ACGGACACGG ATGAGGTGCC GATAGTGCCA TCTGGGCCAA CGCCCATGGA TTTCATGGCC 
GAGTTTGCCA AGCACTGTGC GAACGGAATT GAACAGCTCA AGGTTACTAC ACCGGTGCCA 
TCACCGACTC CCTCGGATTT GGTACCCCTC AACGATCTGG AGGCCAAGCA GAAACTGGAG 
GTGGAGATGA ACGACGTGGA GACCACACTC AATGGCATCC TCAATGAGAT GCAGGACCAG 
CACATGTACA CACCAAACTG TGCCCACATT GATGAGTTCC TCACTCCAGC CGATTATGCC 
ACACTGACTG AGCAGGAGTC GTCCGTTTCG TCGCCACCAA ATCCTTTCGA GCAGAGTTCT 
GTTGCCGAAC CGAAGGACGA AAAGCCCAGT GCCACTGAGG ATCCATTTGA TAATAGCAAC 
TTTACCAATG AACTGATTGG TTTTCAAAAC GATATTCCCT GTTTCGAGAA CATTGAGACA 
CCAGAGGAGG GACAAAATTT GCATTTAGAA CTGACCCAGT TCTTAAGGGA TCTGCAGCCG 
TCACAATCCG ATCCGCCGGC AGGGGAGCAG GTGGAAAAAA TCGAAATGGA GACACAGCCA 
AGCACCGTCC AAATGGATGT CCAATCAAAC CTACCAGTCC AAGCTGCTGA TACACAGCCT 
CAAAATGAGT CCGCTAATCA TTCACCATTT GAATCTCCCA TATCATCACC TCAAGTTCAG 
TCTCAGATCG AATTACAAAT GGAACCTCAA ATCGAGACTC AAATGCAACC TCAAATTGAG 
ACTCGAATGC AACACGATGT GCTAAGCGTG CAATCGCCAC AAAACCATCA GCCTCAAGTA 
CAATCTCATG ACCAATCTCA GATGCAACAC CAAATCCCAC CGCAAATTGA GCCTCAAATT 
GAAGTCCAAA ACCAGGAGCA AAATACTTTA CAGCTCGTGC CGCAAGCACA GCATCAAGTG 
GTTCCACATG TACAACTGCA GTCCCAGTTG GTGCCTCACG TCCAGACGCA GGGCACGACG 
ACGACAGTCA CCTACGAACA GATTTACCAG CAGCACATTG TCCAGCAGAC GGTGCAGCAA 
TCCTCCAACA AGATGCAGGT CATCTCGCTG CCCTCGGCTG GCTCGGAAAC AACACGACAG 
TGGCAGACGC AGCAGTCGCA GCAACAGCAG CAAGTCCAGT GGTCATCGGT TGGCGGACTT 
GAGGCCATAA AGAGTGCACC CGTCGAGAGT GTTGCACCCA CGACCACAAC GTACTACATC 
AGTGCGAGCG ATCTTTATCC GCAGCAGACT GAAACATACA CGATGCTTCA ACCAGCATCC 
AATGAAAGCT ACGTCATTGA GCAGGTGAAT CATCAGCAGC AACATTGCCA GCAACAGATT 
CAGATGTCGC AACAGCAACA TCAACGCCAG CATCAGCAAC AGCAGGCGCA ACAGCAGCAG 
CAACCGATTA TGATACTGAT CCAGCAGCCA GAGATTCAGC AGCCAGTGGC CTCGGCCAGC 
AATCCGCAGC AGGCACCGCT GCACATCTAT AATGCTGCCA CCAGCAACGA TGTGCATCAA 
ATGCAGCACA CGCAACGCCA ACAGATACGG CAGCAGTATC AACACCAGCA GTATCAGCGG 
CTGCAACAGC CGCAACAAAC TCGCGTCGTA CAAAGCCATC CAGTATTGGG AATGCCGGGA 
GCCACTTCCA TATCAACGAA AGTGACCCCC ATTCCCGTCA CAGCGGCCAG GGGTCGGCCG 
CTCACGCTGA AGTGCCGCTT CTGCCATAAC GGGCCGCGAT TCTCCAGCAG CCTGGAGTAC 
AGCCGGCATA TCATCGATCT GCATCCGGCG GTGGCGCCCT TCAATTGTCC ACACTGTCCG 
ATGGCCTTTG CCGGCCGAAC GAAGCGATCG CAGCACATCC TCAGCCATCA TGTGGTGCAG 
CAGTACCAGT GCGGTCAGTG CTCACATGTG TTTCCCGCCC AGAAGGCACT GGACGTTCAC 
ATCCAGCGCT TTCATATGAC CCTGAAAACG GATCCATCGG GCGCTGTGAA GGTGGAGGAC 
GTTCAGCTGC AGATCACCAG CGAACGCAGG CCGCGGGGTA GGCCGTATAA GCCAAGGCTA 
CAGCTTCAAC AGCATCAACT GCAGCCGCAG CAGAAGTTAC AAGTGCAGCA GCATCAGCAG 
CAGCAGAAGG CACTTCAGCA ACTACCCCAA GCACAACACC ACCAACAGCA ACAGCAACCT 
CAACTTCAAC AAGAAGTGCT ACAGCAGGTG CAGCAACTGC AAGTGCAAGT GCAACCGCAG 
CAACACCAGC AACACCAACA AGTGTTGCAA GTGCAGCAAC CACAGCAACA ACCGTTAATG 
CAGCCACAGT CGCCGCATCC ATCAACAGTG GAAATGCAAG CAAGCCCAAC AACGCCACGA 
AAGATTCTTT GCTGTCCCGA TTGCGAAGAC TGCACTTCCG GCCACAGCCA TGCCAACGAA 
CAGTTCGAGG AGCTGCAGAC ACTGCAGGCG CCGCCAACGG TGTTGACTCC GCCATCGACC 
ATAGTCTCGG TACCATCGCC ACAGCCCATG GTGTACTCGC AGCACATAAC AATGCCGTCG 
CCGGAACAAT CCGAACCAGA TTCCACAACC ACGTTGCGAC AGTATCGCAA GCGAGGCGTG 
ATCGTTGGGC CACAGGGTCC GCTGCATTTG GCCACACCGG TGGCCTCGCC ATCGCCCTCG 
TCGTCACCCT CCTCATCGAC GGTGGATCAC ATACCGCCGG CATCTCCGGC TACGCCCGCA 
TCACCAGCTC CGCCTCCGTC GCCCGCCGTC GCGTCCACGG TGCAAGTGAG CGAGCTGCGA 
ACGAGTCACC ATTGTCTGTA CTGCGAGGAG CGATTCACCA ACGAAATTTC CCTGAAGAAG 
CACCATCAGC TGGCGCACGG AGCGCTGACA ACAATGCCAT ATGTGTGCAC AATCTGCAAG 
CGAGGGTATC GCATGCGTAC GGCGCTGCAT CGGCACATGG AGTCGCACGA TGTGGAGGGA 
CGGCCGTACG AGTGCAACAT TTGTCGCGTC CGGTTCCCGC GACCATCGCA ACTGACGCTG 
CACAAGATCA CGGTGCACCT GCTCTCCAAG CCGCACACCT GCGACGAGTG TGGCAAACAG 
TTTGGCACGG AGAGCGCCCT CAAGACGCAC ATTAAGTTCC ACGGAGCTCA CATGAAAACC 
CATTTGCCGC TGGGCGTATT CCGCAACGAG GATGCCGCAT CGAAGTCACC CAGCAACAAC 
AATAGCCACG CCAGCAGTGA CAAGCTGGAG AATCCAGCTA CGCCCATCGA GGAGACACCT 
TTGACGCCGA TGAGCAGTGG CGGCCACTTG TACCACAGCC CAGATGAATA CCCCAATTCG 
GTGGAGAGCT GCGCTGGCAA CAGTGTGGCT CTGGAATCCT ATGCCACAAC GCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mld ( NM_143016.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651273.2 Drosophila melanogaster molting defective (mld), transcript variant E, mRNA.

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CloneID	OFa07278
Clone ID Related Accession (Same CDS sequence)	NM_143016.2
Accession Version	NM_143016.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	molting defective, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On May 8, 2012 this sequence version replaced NM_143016.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGCCA ACCGAAGAAG ACGCAGTGCA TCAGCTGCGA GTTCAATTGC AGCAGAAACA 
GCAACAGCAG CTACAGCAGC AACAGCAGAA GCGATATCAA CTGCACCCTC CAAAGAGCAA 
CTTTCTCCAC CGATTGCTGG TGGCAGTGCT TCAGCTGATG AGGATTTACT GGAAATGCCT 
CGCATTTGCC GATGCTGTTG CAAACGGGAT TTGAAATTAC TCGGCTTATT CGAGGCCAGC 
CAACCAACAC TGGCCAACAC AACAGTGGCA TCAGCATCAG CAGCAGCATC AGTGGCATCG 
AACAAATCAT CGGGAACATT AGCAAAAACA TGTGCGACAG AAGCAGAAAC CGAAACTCCA 
CAGACATTGT CCGCAAATCC AGTAAATCCC GGGACTAAAG CTACATCATC TCCATCAGAA 
GCAGCTACAA CGTTGTCCAC ATCCACACGC CCCGCGTCCG CCTCATTCTC ATCCACCTCC 
TTTTCCACAT CCACGTCTCC CATGAGGCGT CGCACCACTA ACACCACCGC TCCATCCGCT 
TCGCCCGCTC CACGTGGTCG TAGCAGCGAT AATGCCGTCG ACTACACCCT CAGCGGGGCC 
CACAGCAGCA TGGACATTGT CCTCGAGGAG ATGACCATTT GGATGCTCAA TATAAATCGC 
GACGATGGAC TGCCGCAAGA AATCTGCCGG CAGTGCATGG CCCAGTTTTT GATGGTGGCC 
AAGTTCCGGC GGAAATGTGT ACGGATGCAG CAGCGCCTGC AGGACTTTGC CCAGGAGATA 
TCCGACCGCC AGACGGCCAG ACAAGCGAAG CGTCAGCAAA AAGTCCAAAA GAAGACACAC 
GATAGGTGGC TGCAGTTGGA GCCAACCAGC CACGAGGAGC AGCAGCCCAT ACGTAAGCGC 
CGCATGGTAT CCGAATCGGA CGGAGATTCG ACAGCAGCAG CAGCAGCAGC AGAAGCAGCA 
ACAGCAACAG CAACAACACC AGCCAAACAG GTGCGAACGG ATTTCGAGAG TTTGCCACCG 
CCCAAACCAA GTCCTCAACA GCACCGAGTG CGCAAGTATC GAACCCTCTC CATTGACTGT 
GAAATGGAGC GTCCCGCCGC TTCCATCAAT GCCAGCAGCA TGCCCTGGAA GACGAAGGCT 
GCCCGCAAAG AACTTTCGGA GAATTGGGCG TCCAGTTGCT CGACGGCGGT TATGTCGCCC 
AATCGCCAAC GCAGCAGCAC CAGCAGCAGT GAGCACATGA GCGACTTGTC CGTCGGTCCA 
TGCTGTGGCT TCGAAAGCGA CAGCAATCCG TCCTCTACCT CCAACTCGGC GCGGAGAACG 
GTGATACTCG TTAAGCAGCA ATCGCCGGAG AAGACATCGC CCATGTATGA TTTAATGCCG 
CGCAGAGGAC GGCGGCCCAG GAAAGCACCG ATATACATGC CGGCCAAAAG ACAGAGGCGA 
TCGCTGGTTA GAAAGGAGCC GCTATACCGA AAAGCACAGC CAGTCAACGA AGAGAACAAA 
AAGTCTGATC AAAAGGATAT ATATCCGAAG GAATCAAGGA TGGAAGGCCT TGCACCGTCC 
TGTGATGAGG CCATCGACAG CACTTCCGAT GCGCCAATGG AAGTAGCCTC TAATGTGGTC 
CAAAAGAAGT GCGATGAAAT CTGTTTAAAT GGTTTGATAG ACAGTCGCAA CCAGTTAGGC 
GCCATGGAAA TGGAGCCGGT TAATGAAATG ATAGCGTCCA ATGGTGTAGA TAAAGAACTA 
ACCCTCAGCG TTCAAAATAA TAAGGAAACA ACATCCGAAG AGCAAATATT TGAAGCCACC 
AGCGTTATAA AAGAATCAGT TCAACCAGAA CAATCTGCTG AAGATATTCA GGTTAGAACG 
GAAATCGAGT CAATTGCGAA TGACATCAGG GCTGAGGCTG ATCTAGTGCA GTCCGAGGAA 
CTGACCTCAT CCACAGAGAC CAGCGAGTCG CAACCGAAAG CAGTTGAGCA GCTGGAATCT 
ATGGGAAAGC AGGAATCGTA TGCGTTACCA CAAAACATGG AGGCGCATGA ATCCGTCCAA 
CAAACGCAAA CTGTGGACGA ATCAGACTCT GCTGACCAGA GGGAGATCAT AGTGAGCATA 
CCGCTGGAGG TTCTGGATGA GAATCTGCAG CGTCGCCTTT GCGTGGATCC GGAAACTGAG 
GCGGCCAATC GTCAGTCCGC TGATCCCAAG GAGTTTGCCC TGCGTCCCGA TGACGTGAAC 
AACAACGAAA ACGATGAAAA TTTTTGCCAG AGTGCTACAG CAGATAGCTT CAATTCTGCT 
GTGGCACTCC GAACCAATAA AATTGAAGCA ACACCGCCTG GGGGAACGGA TTCAGATGAC 
ACGGACACGG ATGAGGTGCC GATAGTGCCA TCTGGGCCAA CGCCCATGGA TTTCATGGCC 
GAGTTTGCCA AGCACTGTGC GAACGGAATT GAACAGCTCA AGGTTACTAC ACCGGTGCCA 
TCACCGACTC CCTCGGATTT GGTACCCCTC AACGATCTGG AGGCCAAGCA GAAACTGGAG 
GTGGAGATGA ACGACGTGGA GACCACACTC AATGGCATCC TCAATGAGAT GCAGGACCAG 
CACATGTACA CACCAAACTG TGCCCACATT GATGAGTTCC TCACTCCAGC CGATTATGCC 
ACACTGACTG AGCAGGAGTC GTCCGTTTCG TCGCCACCAA ATCCTTTCGA GCAGAGTTCT 
GTTGCCGAAC CGAAGGACGA AAAGCCCAGT GCCACTGAGG ATCCATTTGA TAATAGCAAC 
TTTACCAATG AACTGATTGG TTTTCAAAAC GATATTCCCT GTTTCGAGAA CATTGAGACA 
CCAGAGGAGG GACAAAATTT GCATTTAGAA CTGACCCAGT TCTTAAGGGA TCTGCAGCCG 
TCACAATCCG ATCCGCCGGC AGGGGAGCAG GTGGAAAAAA TCGAAATGGA GACACAGCCA 
AGCACCGTCC AAATGGATGT CCAATCAAAC CTACCAGTCC AAGCTGCTGA TACACAGCCT 
CAAAATGAGT CCGCTAATCA TTCACCATTT GAATCTCCCA TATCATCACC TCAAGTTCAG 
TCTCAGATCG AATTACAAAT GGAACCTCAA ATCGAGACTC AAATGCAACC TCAAATTGAG 
ACTCGAATGC AACACGATGT GCTAAGCGTG CAATCGCCAC AAAACCATCA GCCTCAAGTA 
CAATCTCATG ACCAATCTCA GATGCAACAC CAAATCCCAC CGCAAATTGA GCCTCAAATT 
GAAGTCCAAA ACCAGGAGCA AAATACTTTA CAGCTCGTGC CGCAAGCACA GCATCAAGTG 
GTTCCACATG TACAACTGCA GTCCCAGTTG GTGCCTCACG TCCAGACGCA GGGCACGACG 
ACGACAGTCA CCTACGAACA GATTTACCAG CAGCACATTG TCCAGCAGAC GGTGCAGCAA 
TCCTCCAACA AGATGCAGGT CATCTCGCTG CCCTCGGCTG GCTCGGAAAC AACACGACAG 
TGGCAGACGC AGCAGTCGCA GCAACAGCAG CAAGTCCAGT GGTCATCGGT TGGCGGACTT 
GAGGCCATAA AGAGTGCACC CGTCGAGAGT GTTGCACCCA CGACCACAAC GTACTACATC 
AGTGCGAGCG ATCTTTATCC GCAGCAGACT GAAACATACA CGATGCTTCA ACCAGCATCC 
AATGAAAGCT ACGTCATTGA GCAGGTGAAT CATCAGCAGC AACATTGCCA GCAACAGATT 
CAGATGTCGC AACAGCAACA TCAACGCCAG CATCAGCAAC AGCAGGCGCA ACAGCAGCAG 
CAACCGATTA TGATACTGAT CCAGCAGCCA GAGATTCAGC AGCCAGTGGC CTCGGCCAGC 
AATCCGCAGC AGGCACCGCT GCACATCTAT AATGCTGCCA CCAGCAACGA TGTGCATCAA 
ATGCAGCACA CGCAACGCCA ACAGATACGG CAGCAGTATC AACACCAGCA GTATCAGCGG 
CTGCAACAGC CGCAACAAAC TCGCGTCGTA CAAAGCCATC CAGTATTGGG AATGCCGGGA 
GCCACTTCCA TATCAACGAA AGTGACCCCC ATTCCCGTCA CAGCGGCCAG GGGTCGGCCG 
CTCACGCTGA AGTGCCGCTT CTGCCATAAC GGGCCGCGAT TCTCCAGCAG CCTGGAGTAC 
AGCCGGCATA TCATCGATCT GCATCCGGCG GTGGCGCCCT TCAATTGTCC ACACTGTCCG 
ATGGCCTTTG CCGGCCGAAC GAAGCGATCG CAGCACATCC TCAGCCATCA TGTGGTGCAG 
CAGTACCAGT GCGGTCAGTG CTCACATGTG TTTCCCGCCC AGAAGGCACT GGACGTTCAC 
ATCCAGCGCT TTCATATGAC CCTGAAAACG GATCCATCGG GCGCTGTGAA GGTGGAGGAC 
GTTCAGCTGC AGATCACCAG CGAACGCAGG CCGCGGGGTA GGCCGTATAA GCCAAGGCTA 
CAGCTTCAAC AGCATCAACT GCAGCCGCAG CAGAAGTTAC AAGTGCAGCA GCATCAGCAG 
CAGCAGAAGG CACTTCAGCA ACTACCCCAA GCACAACACC ACCAACAGCA ACAGCAACCT 
CAACTTCAAC AAGAAGTGCT ACAGCAGGTG CAGCAACTGC AAGTGCAAGT GCAACCGCAG 
CAACACCAGC AACACCAACA AGTGTTGCAA GTGCAGCAAC CACAGCAACA ACCGTTAATG 
CAGCCACAGT CGCCGCATCC ATCAAGCACT TCCGGCCACA GCCATGCCAA CGAACAGTTC 
GAGGAGCTGC AGACACTGCA GGCGCCGCCA ACGGTGTTGA CTCCGCCATC GACCATAGTC 
TCGGTACCAT CGCCACAGCC CATGGTGTAC TCGCAGCACA TAACAATGCC GTCGCCGGAA 
CAATCCGAAC CAGATTCCAC AACCACGTTG CGACAGTATC GCAAGCGAGG CGTGATCGTT 
GGGCCACAGG GTCCGCTGCA TTTGGCCACA CCGGTGGCCT CGCCATCGCC CTCGTCGTCA 
CCCTCCTCAT CGACGGTGGA TCACATACCG CCGGCATCTC CGGCTACGCC CGCATCACCA 
GCTCCGCCTC CGTCGCCCGC CGTCGCGTCC ACGGTGCAAG TGAGCGAGCT GCGAACGAGT 
CACCATTGTC TGTACTGCGA GGAGCGATTC ACCAACGAAA TTTCCCTGAA GAAGCACCAT 
CAGCTGGCGC ACGGAGCGCT GACAACAATG CCATATGTGT GCACAATCTG CAAGCGAGGG 
TATCGCATGC GTACGGCGCT GCATCGGCAC ATGGAGTCGC ACGATGTGGA GGGACGGCCG 
TACGAGTGCA ACATTTGTCG CGTCCGGTTC CCGCGACCAT CGCAACTGAC GCTGCACAAG 
ATCACGGTGC ACCTGCTCTC CAAGCCGCAC ACCTGCGACG AGTGTGGCAA ACAGTTTGGC 
ACGGAGAGCG CCCTCAAGAC GCACATTAAG TTCCACGGAG AGCTCGGTTA CCAGTGCGAC 
GGGTGCGATC GGACGTTCGA GTATCTGAAA GAATTGCGCA AACATCGACG CACCCACAGC 
GAGATGTTTT ACAAATGCAA ATTCTGTCCA AGCTCCTTTA TGCGTTTCAC GAATTTTCGG 
GCTCACATGA AAACCCATTT GCCGCTGGGC GTATTCCGCA ACGAGGATGC CGCATCGAAG 
TCACCCAGCA ACAACAATAG CCACGCCAGC AGTGACAAGC TGGAGAATCC AGCTACGCCC 
ATCGAGGAGA CACCTTTGAC GCCGATGAGC AGTGGCGGCC ACTTGTACCA CAGCCCAGAT 
GAATACCCCA ATTCGGTGGA GAGCTGCGCT GGCAACAGTG TGGCTCTGGA ATCCTATGCC 
ACAACGCCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651273.2
CDS	471..6302
Translation	MSANRRRRSASAASSIAAETATAATAATAEAISTAPSKEQLSPPIAGGSASADEDLLEMPRICRCCCKRDLKLLGLFEASQPTLANTTVASASAAASVASNKSSGTLAKTCATEAETETPQTLSANPVNPGTKATSSPSEAATTLSTSTRPASASFSSTSFSTSTSPMRRRTTNTTAPSASPAPRGRSSDNAVDYTLSGAHSSMDIVLEEMTIWMLNINRDDGLPQEICRQCMAQFLMVAKFRRKCVRMQQRLQDFAQEISDRQTARQAKRQQKVQKKTHDRWLQLEPTSHEEQQPIRKRRMVSESDGDSTAAAAAAEAATATATTPAKQVRTDFESLPPPKPSPQQHRVRKYRTLSIDCEMERPAASINASSMPWKTKAARKELSENWASSCSTAVMSPNRQRSSTSSSEHMSDLSVGPCCGFESDSNPSSTSNSARRTVILVKQQSPEKTSPMYDLMPRRGRRPRKAPIYMPAKRQRRSLVRKEPLYRKAQPVNEENKKSDQKDIYPKESRMEGLAPSCDEAIDSTSDAPMEVASNVVQKKCDEICLNGLIDSRNQLGAMEMEPVNEMIASNGVDKELTLSVQNNKETTSEEQIFEATSVIKESVQPEQSAEDIQVRTEIESIANDIRAEADLVQSEELTSSTETSESQPKAVEQLESMGKQESYALPQNMEAHESVQQTQTVDESDSADQREIIVSIPLEVLDENLQRRLCVDPETEAANRQSADPKEFALRPDDVNNNENDENFCQSATADSFNSAVALRTNKIEATPPGGTDSDDTDTDEVPIVPSGPTPMDFMAEFAKHCANGIEQLKVTTPVPSPTPSDLVPLNDLEAKQKLEVEMNDVETTLNGILNEMQDQHMYTPNCAHIDEFLTPADYATLTEQESSVSSPPNPFEQSSVAEPKDEKPSATEDPFDNSNFTNELIGFQNDIPCFENIETPEEGQNLHLELTQFLRDLQPSQSDPPAGEQVEKIEMETQPSTVQMDVQSNLPVQAADTQPQNESANHSPFESPISSPQVQSQIELQMEPQIETQMQPQIETRMQHDVLSVQSPQNHQPQVQSHDQSQMQHQIPPQIEPQIEVQNQEQNTLQLVPQAQHQVVPHVQLQSQLVPHVQTQGTTTTVTYEQIYQQHIVQQTVQQSSNKMQVISLPSAGSETTRQWQTQQSQQQQQVQWSSVGGLEAIKSAPVESVAPTTTTYYISASDLYPQQTETYTMLQPASNESYVIEQVNHQQQHCQQQIQMSQQQHQRQHQQQQAQQQQQPIMILIQQPEIQQPVASASNPQQAPLHIYNAATSNDVHQMQHTQRQQIRQQYQHQQYQRLQQPQQTRVVQSHPVLGMPGATSISTKVTPIPVTAARGRPLTLKCRFCHNGPRFSSSLEYSRHIIDLHPAVAPFNCPHCPMAFAGRTKRSQHILSHHVVQQYQCGQCSHVFPAQKALDVHIQRFHMTLKTDPSGAVKVEDVQLQITSERRPRGRPYKPRLQLQQHQLQPQQKLQVQQHQQQQKALQQLPQAQHHQQQQQPQLQQEVLQQVQQLQVQVQPQQHQQHQQVLQVQQPQQQPLMQPQSPHPSSTSGHSHANEQFEELQTLQAPPTVLTPPSTIVSVPSPQPMVYSQHITMPSPEQSEPDSTTTLRQYRKRGVIVGPQGPLHLATPVASPSPSSSPSSSTVDHIPPASPATPASPAPPPSPAVASTVQVSELRTSHHCLYCEERFTNEISLKKHHQLAHGALTTMPYVCTICKRGYRMRTALHRHMESHDVEGRPYECNICRVRFPRPSQLTLHKITVHLLSKPHTCDECGKQFGTESALKTHIKFHGELGYQCDGCDRTFEYLKELRKHRRTHSEMFYKCKFCPSSFMRFTNFRAHMKTHLPLGVFRNEDAASKSPSNNNSHASSDKLENPATPIEETPLTPMSSGGHLYHSPDEYPNSVESCAGNSVALESYATTP

Target ORF information:

RefSeq Version	NM_143016.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster molting defective (mld), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143016.2

ORF Insert Sequence:

ATGAGTGCCA ACCGAAGAAG ACGCAGTGCA TCAGCTGCGA GTTCAATTGC AGCAGAAACA 
GCAACAGCAG CTACAGCAGC AACAGCAGAA GCGATATCAA CTGCACCCTC CAAAGAGCAA 
CTTTCTCCAC CGATTGCTGG TGGCAGTGCT TCAGCTGATG AGGATTTACT GGAAATGCCT 
CGCATTTGCC GATGCTGTTG CAAACGGGAT TTGAAATTAC TCGGCTTATT CGAGGCCAGC 
CAACCAACAC TGGCCAACAC AACAGTGGCA TCAGCATCAG CAGCAGCATC AGTGGCATCG 
AACAAATCAT CGGGAACATT AGCAAAAACA TGTGCGACAG AAGCAGAAAC CGAAACTCCA 
CAGACATTGT CCGCAAATCC AGTAAATCCC GGGACTAAAG CTACATCATC TCCATCAGAA 
GCAGCTACAA CGTTGTCCAC ATCCACACGC CCCGCGTCCG CCTCATTCTC ATCCACCTCC 
TTTTCCACAT CCACGTCTCC CATGAGGCGT CGCACCACTA ACACCACCGC TCCATCCGCT 
TCGCCCGCTC CACGTGGTCG TAGCAGCGAT AATGCCGTCG ACTACACCCT CAGCGGGGCC 
CACAGCAGCA TGGACATTGT CCTCGAGGAG ATGACCATTT GGATGCTCAA TATAAATCGC 
GACGATGGAC TGCCGCAAGA AATCTGCCGG CAGTGCATGG CCCAGTTTTT GATGGTGGCC 
AAGTTCCGGC GGAAATGTGT ACGGATGCAG CAGCGCCTGC AGGACTTTGC CCAGGAGATA 
TCCGACCGCC AGACGGCCAG ACAAGCGAAG CGTCAGCAAA AAGTCCAAAA GAAGACACAC 
GATAGGTGGC TGCAGTTGGA GCCAACCAGC CACGAGGAGC AGCAGCCCAT ACGTAAGCGC 
CGCATGGTAT CCGAATCGGA CGGAGATTCG ACAGCAGCAG CAGCAGCAGC AGAAGCAGCA 
ACAGCAACAG CAACAACACC AGCCAAACAG GTGCGAACGG ATTTCGAGAG TTTGCCACCG 
CCCAAACCAA GTCCTCAACA GCACCGAGTG CGCAAGTATC GAACCCTCTC CATTGACTGT 
GAAATGGAGC GTCCCGCCGC TTCCATCAAT GCCAGCAGCA TGCCCTGGAA GACGAAGGCT 
GCCCGCAAAG AACTTTCGGA GAATTGGGCG TCCAGTTGCT CGACGGCGGT TATGTCGCCC 
AATCGCCAAC GCAGCAGCAC CAGCAGCAGT GAGCACATGA GCGACTTGTC CGTCGGTCCA 
TGCTGTGGCT TCGAAAGCGA CAGCAATCCG TCCTCTACCT CCAACTCGGC GCGGAGAACG 
GTGATACTCG TTAAGCAGCA ATCGCCGGAG AAGACATCGC CCATGTATGA TTTAATGCCG 
CGCAGAGGAC GGCGGCCCAG GAAAGCACCG ATATACATGC CGGCCAAAAG ACAGAGGCGA 
TCGCTGGTTA GAAAGGAGCC GCTATACCGA AAAGCACAGC CAGTCAACGA AGAGAACAAA 
AAGTCTGATC AAAAGGATAT ATATCCGAAG GAATCAAGGA TGGAAGGCCT TGCACCGTCC 
TGTGATGAGG CCATCGACAG CACTTCCGAT GCGCCAATGG AAGTAGCCTC TAATGTGGTC 
CAAAAGAAGT GCGATGAAAT CTGTTTAAAT GGTTTGATAG ACAGTCGCAA CCAGTTAGGC 
GCCATGGAAA TGGAGCCGGT TAATGAAATG ATAGCGTCCA ATGGTGTAGA TAAAGAACTA 
ACCCTCAGCG TTCAAAATAA TAAGGAAACA ACATCCGAAG AGCAAATATT TGAAGCCACC 
AGCGTTATAA AAGAATCAGT TCAACCAGAA CAATCTGCTG AAGATATTCA GGTTAGAACG 
GAAATCGAGT CAATTGCGAA TGACATCAGG GCTGAGGCTG ATCTAGTGCA GTCCGAGGAA 
CTGACCTCAT CCACAGAGAC CAGCGAGTCG CAACCGAAAG CAGTTGAGCA GCTGGAATCT 
ATGGGAAAGC AGGAATCGTA TGCGTTACCA CAAAACATGG AGGCGCATGA ATCCGTCCAA 
CAAACGCAAA CTGTGGACGA ATCAGACTCT GCTGACCAGA GGGAGATCAT AGTGAGCATA 
CCGCTGGAGG TTCTGGATGA GAATCTGCAG CGTCGCCTTT GCGTGGATCC GGAAACTGAG 
GCGGCCAATC GTCAGTCCGC TGATCCCAAG GAGTTTGCCC TGCGTCCCGA TGACGTGAAC 
AACAACGAAA ACGATGAAAA TTTTTGCCAG AGTGCTACAG CAGATAGCTT CAATTCTGCT 
GTGGCACTCC GAACCAATAA AATTGAAGCA ACACCGCCTG GGGGAACGGA TTCAGATGAC 
ACGGACACGG ATGAGGTGCC GATAGTGCCA TCTGGGCCAA CGCCCATGGA TTTCATGGCC 
GAGTTTGCCA AGCACTGTGC GAACGGAATT GAACAGCTCA AGGTTACTAC ACCGGTGCCA 
TCACCGACTC CCTCGGATTT GGTACCCCTC AACGATCTGG AGGCCAAGCA GAAACTGGAG 
GTGGAGATGA ACGACGTGGA GACCACACTC AATGGCATCC TCAATGAGAT GCAGGACCAG 
CACATGTACA CACCAAACTG TGCCCACATT GATGAGTTCC TCACTCCAGC CGATTATGCC 
ACACTGACTG AGCAGGAGTC GTCCGTTTCG TCGCCACCAA ATCCTTTCGA GCAGAGTTCT 
GTTGCCGAAC CGAAGGACGA AAAGCCCAGT GCCACTGAGG ATCCATTTGA TAATAGCAAC 
TTTACCAATG AACTGATTGG TTTTCAAAAC GATATTCCCT GTTTCGAGAA CATTGAGACA 
CCAGAGGAGG GACAAAATTT GCATTTAGAA CTGACCCAGT TCTTAAGGGA TCTGCAGCCG 
TCACAATCCG ATCCGCCGGC AGGGGAGCAG GTGGAAAAAA TCGAAATGGA GACACAGCCA 
AGCACCGTCC AAATGGATGT CCAATCAAAC CTACCAGTCC AAGCTGCTGA TACACAGCCT 
CAAAATGAGT CCGCTAATCA TTCACCATTT GAATCTCCCA TATCATCACC TCAAGTTCAG 
TCTCAGATCG AATTACAAAT GGAACCTCAA ATCGAGACTC AAATGCAACC TCAAATTGAG 
ACTCGAATGC AACACGATGT GCTAAGCGTG CAATCGCCAC AAAACCATCA GCCTCAAGTA 
CAATCTCATG ACCAATCTCA GATGCAACAC CAAATCCCAC CGCAAATTGA GCCTCAAATT 
GAAGTCCAAA ACCAGGAGCA AAATACTTTA CAGCTCGTGC CGCAAGCACA GCATCAAGTG 
GTTCCACATG TACAACTGCA GTCCCAGTTG GTGCCTCACG TCCAGACGCA GGGCACGACG 
ACGACAGTCA CCTACGAACA GATTTACCAG CAGCACATTG TCCAGCAGAC GGTGCAGCAA 
TCCTCCAACA AGATGCAGGT CATCTCGCTG CCCTCGGCTG GCTCGGAAAC AACACGACAG 
TGGCAGACGC AGCAGTCGCA GCAACAGCAG CAAGTCCAGT GGTCATCGGT TGGCGGACTT 
GAGGCCATAA AGAGTGCACC CGTCGAGAGT GTTGCACCCA CGACCACAAC GTACTACATC 
AGTGCGAGCG ATCTTTATCC GCAGCAGACT GAAACATACA CGATGCTTCA ACCAGCATCC 
AATGAAAGCT ACGTCATTGA GCAGGTGAAT CATCAGCAGC AACATTGCCA GCAACAGATT 
CAGATGTCGC AACAGCAACA TCAACGCCAG CATCAGCAAC AGCAGGCGCA ACAGCAGCAG 
CAACCGATTA TGATACTGAT CCAGCAGCCA GAGATTCAGC AGCCAGTGGC CTCGGCCAGC 
AATCCGCAGC AGGCACCGCT GCACATCTAT AATGCTGCCA CCAGCAACGA TGTGCATCAA 
ATGCAGCACA CGCAACGCCA ACAGATACGG CAGCAGTATC AACACCAGCA GTATCAGCGG 
CTGCAACAGC CGCAACAAAC TCGCGTCGTA CAAAGCCATC CAGTATTGGG AATGCCGGGA 
GCCACTTCCA TATCAACGAA AGTGACCCCC ATTCCCGTCA CAGCGGCCAG GGGTCGGCCG 
CTCACGCTGA AGTGCCGCTT CTGCCATAAC GGGCCGCGAT TCTCCAGCAG CCTGGAGTAC 
AGCCGGCATA TCATCGATCT GCATCCGGCG GTGGCGCCCT TCAATTGTCC ACACTGTCCG 
ATGGCCTTTG CCGGCCGAAC GAAGCGATCG CAGCACATCC TCAGCCATCA TGTGGTGCAG 
CAGTACCAGT GCGGTCAGTG CTCACATGTG TTTCCCGCCC AGAAGGCACT GGACGTTCAC 
ATCCAGCGCT TTCATATGAC CCTGAAAACG GATCCATCGG GCGCTGTGAA GGTGGAGGAC 
GTTCAGCTGC AGATCACCAG CGAACGCAGG CCGCGGGGTA GGCCGTATAA GCCAAGGCTA 
CAGCTTCAAC AGCATCAACT GCAGCCGCAG CAGAAGTTAC AAGTGCAGCA GCATCAGCAG 
CAGCAGAAGG CACTTCAGCA ACTACCCCAA GCACAACACC ACCAACAGCA ACAGCAACCT 
CAACTTCAAC AAGAAGTGCT ACAGCAGGTG CAGCAACTGC AAGTGCAAGT GCAACCGCAG 
CAACACCAGC AACACCAACA AGTGTTGCAA GTGCAGCAAC CACAGCAACA ACCGTTAATG 
CAGCCACAGT CGCCGCATCC ATCAAGCACT TCCGGCCACA GCCATGCCAA CGAACAGTTC 
GAGGAGCTGC AGACACTGCA GGCGCCGCCA ACGGTGTTGA CTCCGCCATC GACCATAGTC 
TCGGTACCAT CGCCACAGCC CATGGTGTAC TCGCAGCACA TAACAATGCC GTCGCCGGAA 
CAATCCGAAC CAGATTCCAC AACCACGTTG CGACAGTATC GCAAGCGAGG CGTGATCGTT 
GGGCCACAGG GTCCGCTGCA TTTGGCCACA CCGGTGGCCT CGCCATCGCC CTCGTCGTCA 
CCCTCCTCAT CGACGGTGGA TCACATACCG CCGGCATCTC CGGCTACGCC CGCATCACCA 
GCTCCGCCTC CGTCGCCCGC CGTCGCGTCC ACGGTGCAAG TGAGCGAGCT GCGAACGAGT 
CACCATTGTC TGTACTGCGA GGAGCGATTC ACCAACGAAA TTTCCCTGAA GAAGCACCAT 
CAGCTGGCGC ACGGAGCGCT GACAACAATG CCATATGTGT GCACAATCTG CAAGCGAGGG 
TATCGCATGC GTACGGCGCT GCATCGGCAC ATGGAGTCGC ACGATGTGGA GGGACGGCCG 
TACGAGTGCA ACATTTGTCG CGTCCGGTTC CCGCGACCAT CGCAACTGAC GCTGCACAAG 
ATCACGGTGC ACCTGCTCTC CAAGCCGCAC ACCTGCGACG AGTGTGGCAA ACAGTTTGGC 
ACGGAGAGCG CCCTCAAGAC GCACATTAAG TTCCACGGAG AGCTCGGTTA CCAGTGCGAC 
GGGTGCGATC GGACGTTCGA GTATCTGAAA GAATTGCGCA AACATCGACG CACCCACAGC 
GAGATGTTTT ACAAATGCAA ATTCTGTCCA AGCTCCTTTA TGCGTTTCAC GAATTTTCGG 
GCTCACATGA AAACCCATTT GCCGCTGGGC GTATTCCGCA ACGAGGATGC CGCATCGAAG 
TCACCCAGCA ACAACAATAG CCACGCCAGC AGTGACAAGC TGGAGAATCC AGCTACGCCC 
ATCGAGGAGA CACCTTTGAC GCCGATGAGC AGTGGCGGCC ACTTGTACCA CAGCCCAGAT 
GAATACCCCA ATTCGGTGGA GAGCTGCGCT GGCAACAGTG TGGCTCTGGA ATCCTATGCC 
ACAACGCCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mld ( NM_001260360.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247289.1 Drosophila melanogaster molting defective (mld), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa08035
Clone ID Related Accession (Same CDS sequence)	NM_001260360.1
Accession Version	NM_001260360.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5898bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	molting defective, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGCCA ACCGAAGAAG ACGCAGTGCA TCAGCTGCGA GTTCAATTGC AGCAGAAACA 
GCAACAGCAG CTACAGCAGC AACAGCAGAA GCGATATCAA CTGCACCCTC CAAAGAGCAA 
CTTTCTCCAC CGATTGCTGG TGGCAGTGCT TCAGCTGATG AGGATTTACT GGAAATGCCT 
CGCATTTGCC GATGCTGTTG CAAACGGGAT TTGAAATTAC TCGGCTTATT CGAGGCCAGC 
CAACCAACAC TGGCCAACAC AACAGTGGCA TCAGCATCAG CAGCAGCATC AGTGGCATCG 
AACAAATCAT CGGGAACATT AGCAAAAACA TGTGCGACAG AAGCAGAAAC CGAAACTCCA 
CAGACATTGT CCGCAAATCC AGTAAATCCC GGGACTAAAG CTACATCATC TCCATCAGAA 
GCAGCTACAA CGTTGTCCAC ATCCACACGC CCCGCGTCCG CCTCATTCTC ATCCACCTCC 
TTTTCCACAT CCACGTCTCC CATGAGGCGT CGCACCACTA ACACCACCGC TCCATCCGCT 
TCGCCCGCTC CACGTGGTCG TAGCAGCGAT AATGCCGTCG ACTACACCCT CAGCGGGGCC 
CACAGCAGCA TGGACATTGT CCTCGAGGAG ATGACCATTT GGATGCTCAA TATAAATCGC 
GACGATGGAC TGCCGCAAGA AATCTGCCGG CAGTGCATGG CCCAGTTTTT GATGGTGGCC 
AAGTTCCGGC GGAAATGTGT ACGGATGCAG CAGCGCCTGC AGGACTTTGC CCAGGAGATA 
TCCGACCGCC AGACGGCCAG ACAAGCGAAG CGTCAGCAAA AAGTCCAAAA GAAGACACAC 
GATAGGTGGC TGCAGTTGGA GCCAACCAGC CACGAGGAGC AGCAGCCCAT ACGTAAGCGC 
CGCATGGTAT CCGAATCGGA CGGAGATTCG ACAGCAGCAG CAGCAGCAGC AGAAGCAGCA 
ACAGCAACAG CAACAACACC AGCCAAACAG GTGCGAACGG ATTTCGAGAG TTTGCCACCG 
CCCAAACCAA GTCCTCAACA GCACCGAGTG CGCAAGTATC GAACCCTCTC CATTGACTGT 
GAAATGGAGC GTCCCGCCGC TTCCATCAAT GCCAGCAGCA TGCCCTGGAA GACGAAGGCT 
GCCCGCAAAG AACTTTCGGA GAATTGGGCG TCCAGTTGCT CGACGGCGGT TATGTCGCCC 
AATCGCCAAC GCAGCAGCAC CAGCAGCAGT GAGCACATGA GCGACTTGTC CGTCGGTCCA 
TGCTGTGGCT TCGAAAGCGA CAGCAATCCG TCCTCTACCT CCAACTCGGC GCGGAGAACG 
GTGATACTCG TTAAGCAGCA ATCGCCGGAG AAGACATCGC CCATGTATGA TTTAATGCCG 
CGCAGAGGAC GGCGGCCCAG GAAAGCACCG ATATACATGC CGGCCAAAAG ACAGAGGCGA 
TCGCTGGTTA GAAAGGAGCC GCTATACCGA AAAGCACAGC CAGTCAACGA AGAGAACAAA 
AAGTCTGATC AAAAGGATAT ATATCCGAAG GAATCAAGGA TGGAAGGCCT TGCACCGTCC 
TGTGATGAGG CCATCGACAG CACTTCCGAT GCGCCAATGG AAGTAGCCTC TAATGTGGTC 
CAAAAGAAGT GCGATGAAAT CTGTTTAAAT GGTTTGATAG ACAGTCGCAA CCAGTTAGGC 
GCCATGGAAA TGGAGCCGGT TAATGAAATG ATAGCGTCCA ATGGTGTAGA TAAAGAACTA 
ACCCTCAGCG TTCAAAATAA TAAGGAAACA ACATCCGAAG AGCAAATATT TGAAGCCACC 
AGCGTTATAA AAGAATCAGT TCAACCAGAA CAATCTGCTG AAGATATTCA GGTTAGAACG 
GAAATCGAGT CAATTGCGAA TGACATCAGG GCTGAGGCTG ATCTAGTGCA GTCCGAGGAA 
CTGACCTCAT CCACAGAGAC CAGCGAGTCG CAACCGAAAG CAGTTGAGCA GCTGGAATCT 
ATGGGAAAGC AGGAATCGTA TGCGTTACCA CAAAACATGG AGGCGCATGA ATCCGTCCAA 
CAAACGCAAA CTGTGGACGA ATCAGACTCT GCTGACCAGA GGGAGATCAT AGTGAGCATA 
CCGCTGGAGG TTCTGGATGA GAATCTGCAG CGTCGCCTTT GCGTGGATCC GGAAACTGAG 
GCGGCCAATC GTCAGTCCGC TGATCCCAAG GAGTTTGCCC TGCGTCCCGA TGACGTGAAC 
AACAACGAAA ACGATGAAAA TTTTTGCCAG AGTGCTACAG CAGATAGCTT CAATTCTGCT 
GTGGCACTCC GAACCAATAA AATTGAAGCA ACACCGCCTG GGGGAACGGA TTCAGATGAC 
ACGGACACGG ATGAGGTGCC GATAGTGCCA TCTGGGCCAA CGCCCATGGA TTTCATGGCC 
GAGTTTGCCA AGCACTGTGC GAACGGAATT GAACAGCTCA AGGTTACTAC ACCGGTGCCA 
TCACCGACTC CCTCGGATTT GGTACCCCTC AACGATCTGG AGGCCAAGCA GAAACTGGAG 
GTGGAGATGA ACGACGTGGA GACCACACTC AATGGCATCC TCAATGAGAT GCAGGACCAG 
CACATGTACA CACCAAACTG TGCCCACATT GATGAGTTCC TCACTCCAGC CGATTATGCC 
ACACTGACTG AGCAGGAGTC GTCCGTTTCG TCGCCACCAA ATCCTTTCGA GCAGAGTTCT 
GTTGCCGAAC CGAAGGACGA AAAGCCCAGT GCCACTGAGG ATCCATTTGA TAATAGCAAC 
TTTACCAATG AACTGATTGG TTTTCAAAAC GATATTCCCT GTTTCGAGAA CATTGAGACA 
CCAGAGGAGG GACAAAATTT GCATTTAGAA CTGACCCAGT TCTTAAGGGA TCTGCAGCCG 
TCACAATCCG ATCCGCCGGC AGGGGAGCAG GTGGAAAAAA TCGAAATGGA GACACAGCCA 
AGCACCGTCC AAATGGATGT CCAATCAAAC CTACCAGTCC AAGCTGCTGA TACACAGCCT 
CAAAATGAGT CCGCTAATCA TTCACCATTT GAATCTCCCA TATCATCACC TCAAGTTCAG 
TCTCAGATCG AATTACAAAT GGAACCTCAA ATCGAGACTC AAATGCAACC TCAAATTGAG 
ACTCGAATGC AACACGATGT GCTAAGCGTG CAATCGCCAC AAAACCATCA GCCTCAAGTA 
CAATCTCATG ACCAATCTCA GATGCAACAC CAAATCCCAC CGCAAATTGA GCCTCAAATT 
GAAGTCCAAA ACCAGGAGCA AAATACTTTA CAGCTCGTGC CGCAAGCACA GCATCAAGTG 
GTTCCACATG TACAACTGCA GTCCCAGTTG GTGCCTCACG TCCAGACGCA GGGCACGACG 
ACGACAGTCA CCTACGAACA GATTTACCAG CAGCACATTG TCCAGCAGAC GGTGCAGCAA 
TCCTCCAACA AGATGCAGGT CATCTCGCTG CCCTCGGCTG GCTCGGAAAC AACACGACAG 
TGGCAGACGC AGCAGTCGCA GCAACAGCAG CAAGTCCAGT GGTCATCGGT TGGCGGACTT 
GAGGCCATAA AGAGTGCACC CGTCGAGAGT GTTGCACCCA CGACCACAAC GTACTACATC 
AGTGCGAGCG ATCTTTATCC GCAGCAGACT GAAACATACA CGATGCTTCA ACCAGCATCC 
AATGAAAGCT ACGTCATTGA GCAGGTGAAT CATCAGCAGC AACATTGCCA GCAACAGATT 
CAGATGTCGC AACAGCAACA TCAACGCCAG CATCAGCAAC AGCAGGCGCA ACAGCAGCAG 
CAACCGATTA TGATACTGAT CCAGCAGCCA GAGATTCAGC AGCCAGTGGC CTCGGCCAGC 
AATCCGCAGC AGGCACCGCT GCACATCTAT AATGCTGCCA CCAGCAACGA TGTGCATCAA 
ATGCAGCACA CGCAACGCCA ACAGATACGG CAGCAGTATC AACACCAGCA GTATCAGCGG 
CTGCAACAGC CGCAACAAAC TCGCGTCGTA CAAAGCCATC CAGTATTGGG AATGCCGGGA 
GCCACTTCCA TATCAACGAA AGTGACCCCC ATTCCCGTCA CAGCGGCCAG GGGTCGGCCG 
CTCACGCTGA AGTGCCGCTT CTGCCATAAC GGGCCGCGAT TCTCCAGCAG CCTGGAGTAC 
AGCCGGCATA TCATCGATCT GCATCCGGCG GTGGCGCCCT TCAATTGTCC ACACTGTCCG 
ATGGCCTTTG CCGGCCGAAC GAAGCGATCG CAGCACATCC TCAGCCATCA TGTGGTGCAG 
CAGTACCAGT GCGGTCAGTG CTCACATGTG TTTCCCGCCC AGAAGGCACT GGACGTTCAC 
ATCCAGCGCT TTCATATGAC CCTGAAAACG GATCCATCGG GCGCTGTGAA GGTGGAGGAC 
GTTCAGCTGC AGATCACCAG CGAACGCAGG CCGCGGGGTA GGCCGTATAA GCCAAGGCTA 
CAGCTTCAAC AGCATCAACT GCAGCCGCAG CAGAAGTTAC AAGTGCAGCA GCATCAGCAG 
CAGCAGAAGG CACTTCAGCA ACTACCCCAA GCACAACACC ACCAACAGCA ACAGCAACCT 
CAACTTCAAC AAGAAGTGCT ACAGCAGGTG CAGCAACTGC AAGTGCAAGT GCAACCGCAG 
CAACACCAGC AACACCAACA AGTGTTGCAA GTGCAGCAAC CACAGCAACA ACCGTTAATG 
CAGCCACAGT CGCCGCATCC ATCAACAGTG GAAATGCAAG CAAGCCCAAC AACGCCACGA 
AAGATTCTTT GCTGTCCCGA TTGCGAAGAC TGCACTTCCG GCCACAGCCA TGCCAACGAA 
CAGTTCGAGG AGCTGCAGAC ACTGCAGGCG CCGCCAACGG TGTTGACTCC GCCATCGACC 
ATAGTCTCGG TACCATCGCC ACAGCCCATG GTGTACTCGC AGCACATAAC AATGCCGTCG 
CCGGAACAAT CCGAACCAGA TTCCACAACC ACGTTGCGAC AGTATCGCAA GCGAGGCGTG 
ATCGTTGGGC CACAGGGTCC GCTGCATTTG GCCACACCGG TGGCCTCGCC ATCGCCCTCG 
TCGTCACCCT CCTCATCGAC GGTGGATCAC ATACCGCCGG CATCTCCGGC TACGCCCGCA 
TCACCAGCTC CGCCTCCGTC GCCCGCCGTC GCGTCCACGG TGCAAGTGAG CGAGCTGCGA 
ACGAGTCACC ATTGTCTGTA CTGCGAGGAG CGATTCACCA ACGAAATTTC CCTGAAGAAG 
CACCATCAGC TGGCGCACGG AGCGCTGACA ACAATGCCAT ATGTGTGCAC AATCTGCAAG 
CGAGGGTATC GCATGCGTAC GGCGCTGCAT CGGCACATGG AGTCGCACGA TGTGGAGGGA 
CGGCCGTACG AGTGCAACAT TTGTCGCGTC CGGTTCCCGC GACCATCGCA ACTGACGCTG 
CACAAGATCA CGGTGCACCT GCTCTCCAAG CCGCACACCT GCGACGAGTG TGGCAAACAG 
TTTGGCACGG AGAGCGCCCT CAAGACGCAC ATTAAGTTCC ACGGAGAGCT CGGTTACCAG 
TGCGACGGGT GCGATCGGAC GTTCGAGTAT CTGAAAGAAT TGCGCAAACA TCGACGCACC 
CACAGCGAGA TGTTTTACAA ATGCAAATTC TGTCCAAGCT CCTTTATGCG TTTCACGAAT 
TTTCGGGCTC ACATGAAAAC CCATTTGCCG CTGGGCGTAT TCCGCAACGA GGATGCCGCA 
TCGAAGTCAC CCAGCAACAA CAATAGCCAC GCCAGCAGTG ACAAGCTGGA GAATCCAGCT 
ACGCCCATCG AGGAGACACC TTTGACGCCG ATGAGCAGTG GCGGCCACTT GTACCACAGC 
CCAGATGAAT ACCCCAATTC GGTGGAGAGC TGCGCTGGCA ACAGTGTGGC TCTGGAATCC 
TATGCCACAA CGCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247289.1
CDS	474..6371
Translation	MSANRRRRSASAASSIAAETATAATAATAEAISTAPSKEQLSPPIAGGSASADEDLLEMPRICRCCCKRDLKLLGLFEASQPTLANTTVASASAAASVASNKSSGTLAKTCATEAETETPQTLSANPVNPGTKATSSPSEAATTLSTSTRPASASFSSTSFSTSTSPMRRRTTNTTAPSASPAPRGRSSDNAVDYTLSGAHSSMDIVLEEMTIWMLNINRDDGLPQEICRQCMAQFLMVAKFRRKCVRMQQRLQDFAQEISDRQTARQAKRQQKVQKKTHDRWLQLEPTSHEEQQPIRKRRMVSESDGDSTAAAAAAEAATATATTPAKQVRTDFESLPPPKPSPQQHRVRKYRTLSIDCEMERPAASINASSMPWKTKAARKELSENWASSCSTAVMSPNRQRSSTSSSEHMSDLSVGPCCGFESDSNPSSTSNSARRTVILVKQQSPEKTSPMYDLMPRRGRRPRKAPIYMPAKRQRRSLVRKEPLYRKAQPVNEENKKSDQKDIYPKESRMEGLAPSCDEAIDSTSDAPMEVASNVVQKKCDEICLNGLIDSRNQLGAMEMEPVNEMIASNGVDKELTLSVQNNKETTSEEQIFEATSVIKESVQPEQSAEDIQVRTEIESIANDIRAEADLVQSEELTSSTETSESQPKAVEQLESMGKQESYALPQNMEAHESVQQTQTVDESDSADQREIIVSIPLEVLDENLQRRLCVDPETEAANRQSADPKEFALRPDDVNNNENDENFCQSATADSFNSAVALRTNKIEATPPGGTDSDDTDTDEVPIVPSGPTPMDFMAEFAKHCANGIEQLKVTTPVPSPTPSDLVPLNDLEAKQKLEVEMNDVETTLNGILNEMQDQHMYTPNCAHIDEFLTPADYATLTEQESSVSSPPNPFEQSSVAEPKDEKPSATEDPFDNSNFTNELIGFQNDIPCFENIETPEEGQNLHLELTQFLRDLQPSQSDPPAGEQVEKIEMETQPSTVQMDVQSNLPVQAADTQPQNESANHSPFESPISSPQVQSQIELQMEPQIETQMQPQIETRMQHDVLSVQSPQNHQPQVQSHDQSQMQHQIPPQIEPQIEVQNQEQNTLQLVPQAQHQVVPHVQLQSQLVPHVQTQGTTTTVTYEQIYQQHIVQQTVQQSSNKMQVISLPSAGSETTRQWQTQQSQQQQQVQWSSVGGLEAIKSAPVESVAPTTTTYYISASDLYPQQTETYTMLQPASNESYVIEQVNHQQQHCQQQIQMSQQQHQRQHQQQQAQQQQQPIMILIQQPEIQQPVASASNPQQAPLHIYNAATSNDVHQMQHTQRQQIRQQYQHQQYQRLQQPQQTRVVQSHPVLGMPGATSISTKVTPIPVTAARGRPLTLKCRFCHNGPRFSSSLEYSRHIIDLHPAVAPFNCPHCPMAFAGRTKRSQHILSHHVVQQYQCGQCSHVFPAQKALDVHIQRFHMTLKTDPSGAVKVEDVQLQITSERRPRGRPYKPRLQLQQHQLQPQQKLQVQQHQQQQKALQQLPQAQHHQQQQQPQLQQEVLQQVQQLQVQVQPQQHQQHQQVLQVQQPQQQPLMQPQSPHPSTVEMQASPTTPRKILCCPDCEDCTSGHSHANEQFEELQTLQAPPTVLTPPSTIVSVPSPQPMVYSQHITMPSPEQSEPDSTTTLRQYRKRGVIVGPQGPLHLATPVASPSPSSSPSSSTVDHIPPASPATPASPAPPPSPAVASTVQVSELRTSHHCLYCEERFTNEISLKKHHQLAHGALTTMPYVCTICKRGYRMRTALHRHMESHDVEGRPYECNICRVRFPRPSQLTLHKITVHLLSKPHTCDECGKQFGTESALKTHIKFHGELGYQCDGCDRTFEYLKELRKHRRTHSEMFYKCKFCPSSFMRFTNFRAHMKTHLPLGVFRNEDAASKSPSNNNSHASSDKLENPATPIEETPLTPMSSGGHLYHSPDEYPNSVESCAGNSVALESYATTP

Target ORF information:

RefSeq Version	NM_001260360.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster molting defective (mld), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260360.1

ORF Insert Sequence:

ATGAGTGCCA ACCGAAGAAG ACGCAGTGCA TCAGCTGCGA GTTCAATTGC AGCAGAAACA 
GCAACAGCAG CTACAGCAGC AACAGCAGAA GCGATATCAA CTGCACCCTC CAAAGAGCAA 
CTTTCTCCAC CGATTGCTGG TGGCAGTGCT TCAGCTGATG AGGATTTACT GGAAATGCCT 
CGCATTTGCC GATGCTGTTG CAAACGGGAT TTGAAATTAC TCGGCTTATT CGAGGCCAGC 
CAACCAACAC TGGCCAACAC AACAGTGGCA TCAGCATCAG CAGCAGCATC AGTGGCATCG 
AACAAATCAT CGGGAACATT AGCAAAAACA TGTGCGACAG AAGCAGAAAC CGAAACTCCA 
CAGACATTGT CCGCAAATCC AGTAAATCCC GGGACTAAAG CTACATCATC TCCATCAGAA 
GCAGCTACAA CGTTGTCCAC ATCCACACGC CCCGCGTCCG CCTCATTCTC ATCCACCTCC 
TTTTCCACAT CCACGTCTCC CATGAGGCGT CGCACCACTA ACACCACCGC TCCATCCGCT 
TCGCCCGCTC CACGTGGTCG TAGCAGCGAT AATGCCGTCG ACTACACCCT CAGCGGGGCC 
CACAGCAGCA TGGACATTGT CCTCGAGGAG ATGACCATTT GGATGCTCAA TATAAATCGC 
GACGATGGAC TGCCGCAAGA AATCTGCCGG CAGTGCATGG CCCAGTTTTT GATGGTGGCC 
AAGTTCCGGC GGAAATGTGT ACGGATGCAG CAGCGCCTGC AGGACTTTGC CCAGGAGATA 
TCCGACCGCC AGACGGCCAG ACAAGCGAAG CGTCAGCAAA AAGTCCAAAA GAAGACACAC 
GATAGGTGGC TGCAGTTGGA GCCAACCAGC CACGAGGAGC AGCAGCCCAT ACGTAAGCGC 
CGCATGGTAT CCGAATCGGA CGGAGATTCG ACAGCAGCAG CAGCAGCAGC AGAAGCAGCA 
ACAGCAACAG CAACAACACC AGCCAAACAG GTGCGAACGG ATTTCGAGAG TTTGCCACCG 
CCCAAACCAA GTCCTCAACA GCACCGAGTG CGCAAGTATC GAACCCTCTC CATTGACTGT 
GAAATGGAGC GTCCCGCCGC TTCCATCAAT GCCAGCAGCA TGCCCTGGAA GACGAAGGCT 
GCCCGCAAAG AACTTTCGGA GAATTGGGCG TCCAGTTGCT CGACGGCGGT TATGTCGCCC 
AATCGCCAAC GCAGCAGCAC CAGCAGCAGT GAGCACATGA GCGACTTGTC CGTCGGTCCA 
TGCTGTGGCT TCGAAAGCGA CAGCAATCCG TCCTCTACCT CCAACTCGGC GCGGAGAACG 
GTGATACTCG TTAAGCAGCA ATCGCCGGAG AAGACATCGC CCATGTATGA TTTAATGCCG 
CGCAGAGGAC GGCGGCCCAG GAAAGCACCG ATATACATGC CGGCCAAAAG ACAGAGGCGA 
TCGCTGGTTA GAAAGGAGCC GCTATACCGA AAAGCACAGC CAGTCAACGA AGAGAACAAA 
AAGTCTGATC AAAAGGATAT ATATCCGAAG GAATCAAGGA TGGAAGGCCT TGCACCGTCC 
TGTGATGAGG CCATCGACAG CACTTCCGAT GCGCCAATGG AAGTAGCCTC TAATGTGGTC 
CAAAAGAAGT GCGATGAAAT CTGTTTAAAT GGTTTGATAG ACAGTCGCAA CCAGTTAGGC 
GCCATGGAAA TGGAGCCGGT TAATGAAATG ATAGCGTCCA ATGGTGTAGA TAAAGAACTA 
ACCCTCAGCG TTCAAAATAA TAAGGAAACA ACATCCGAAG AGCAAATATT TGAAGCCACC 
AGCGTTATAA AAGAATCAGT TCAACCAGAA CAATCTGCTG AAGATATTCA GGTTAGAACG 
GAAATCGAGT CAATTGCGAA TGACATCAGG GCTGAGGCTG ATCTAGTGCA GTCCGAGGAA 
CTGACCTCAT CCACAGAGAC CAGCGAGTCG CAACCGAAAG CAGTTGAGCA GCTGGAATCT 
ATGGGAAAGC AGGAATCGTA TGCGTTACCA CAAAACATGG AGGCGCATGA ATCCGTCCAA 
CAAACGCAAA CTGTGGACGA ATCAGACTCT GCTGACCAGA GGGAGATCAT AGTGAGCATA 
CCGCTGGAGG TTCTGGATGA GAATCTGCAG CGTCGCCTTT GCGTGGATCC GGAAACTGAG 
GCGGCCAATC GTCAGTCCGC TGATCCCAAG GAGTTTGCCC TGCGTCCCGA TGACGTGAAC 
AACAACGAAA ACGATGAAAA TTTTTGCCAG AGTGCTACAG CAGATAGCTT CAATTCTGCT 
GTGGCACTCC GAACCAATAA AATTGAAGCA ACACCGCCTG GGGGAACGGA TTCAGATGAC 
ACGGACACGG ATGAGGTGCC GATAGTGCCA TCTGGGCCAA CGCCCATGGA TTTCATGGCC 
GAGTTTGCCA AGCACTGTGC GAACGGAATT GAACAGCTCA AGGTTACTAC ACCGGTGCCA 
TCACCGACTC CCTCGGATTT GGTACCCCTC AACGATCTGG AGGCCAAGCA GAAACTGGAG 
GTGGAGATGA ACGACGTGGA GACCACACTC AATGGCATCC TCAATGAGAT GCAGGACCAG 
CACATGTACA CACCAAACTG TGCCCACATT GATGAGTTCC TCACTCCAGC CGATTATGCC 
ACACTGACTG AGCAGGAGTC GTCCGTTTCG TCGCCACCAA ATCCTTTCGA GCAGAGTTCT 
GTTGCCGAAC CGAAGGACGA AAAGCCCAGT GCCACTGAGG ATCCATTTGA TAATAGCAAC 
TTTACCAATG AACTGATTGG TTTTCAAAAC GATATTCCCT GTTTCGAGAA CATTGAGACA 
CCAGAGGAGG GACAAAATTT GCATTTAGAA CTGACCCAGT TCTTAAGGGA TCTGCAGCCG 
TCACAATCCG ATCCGCCGGC AGGGGAGCAG GTGGAAAAAA TCGAAATGGA GACACAGCCA 
AGCACCGTCC AAATGGATGT CCAATCAAAC CTACCAGTCC AAGCTGCTGA TACACAGCCT 
CAAAATGAGT CCGCTAATCA TTCACCATTT GAATCTCCCA TATCATCACC TCAAGTTCAG 
TCTCAGATCG AATTACAAAT GGAACCTCAA ATCGAGACTC AAATGCAACC TCAAATTGAG 
ACTCGAATGC AACACGATGT GCTAAGCGTG CAATCGCCAC AAAACCATCA GCCTCAAGTA 
CAATCTCATG ACCAATCTCA GATGCAACAC CAAATCCCAC CGCAAATTGA GCCTCAAATT 
GAAGTCCAAA ACCAGGAGCA AAATACTTTA CAGCTCGTGC CGCAAGCACA GCATCAAGTG 
GTTCCACATG TACAACTGCA GTCCCAGTTG GTGCCTCACG TCCAGACGCA GGGCACGACG 
ACGACAGTCA CCTACGAACA GATTTACCAG CAGCACATTG TCCAGCAGAC GGTGCAGCAA 
TCCTCCAACA AGATGCAGGT CATCTCGCTG CCCTCGGCTG GCTCGGAAAC AACACGACAG 
TGGCAGACGC AGCAGTCGCA GCAACAGCAG CAAGTCCAGT GGTCATCGGT TGGCGGACTT 
GAGGCCATAA AGAGTGCACC CGTCGAGAGT GTTGCACCCA CGACCACAAC GTACTACATC 
AGTGCGAGCG ATCTTTATCC GCAGCAGACT GAAACATACA CGATGCTTCA ACCAGCATCC 
AATGAAAGCT ACGTCATTGA GCAGGTGAAT CATCAGCAGC AACATTGCCA GCAACAGATT 
CAGATGTCGC AACAGCAACA TCAACGCCAG CATCAGCAAC AGCAGGCGCA ACAGCAGCAG 
CAACCGATTA TGATACTGAT CCAGCAGCCA GAGATTCAGC AGCCAGTGGC CTCGGCCAGC 
AATCCGCAGC AGGCACCGCT GCACATCTAT AATGCTGCCA CCAGCAACGA TGTGCATCAA 
ATGCAGCACA CGCAACGCCA ACAGATACGG CAGCAGTATC AACACCAGCA GTATCAGCGG 
CTGCAACAGC CGCAACAAAC TCGCGTCGTA CAAAGCCATC CAGTATTGGG AATGCCGGGA 
GCCACTTCCA TATCAACGAA AGTGACCCCC ATTCCCGTCA CAGCGGCCAG GGGTCGGCCG 
CTCACGCTGA AGTGCCGCTT CTGCCATAAC GGGCCGCGAT TCTCCAGCAG CCTGGAGTAC 
AGCCGGCATA TCATCGATCT GCATCCGGCG GTGGCGCCCT TCAATTGTCC ACACTGTCCG 
ATGGCCTTTG CCGGCCGAAC GAAGCGATCG CAGCACATCC TCAGCCATCA TGTGGTGCAG 
CAGTACCAGT GCGGTCAGTG CTCACATGTG TTTCCCGCCC AGAAGGCACT GGACGTTCAC 
ATCCAGCGCT TTCATATGAC CCTGAAAACG GATCCATCGG GCGCTGTGAA GGTGGAGGAC 
GTTCAGCTGC AGATCACCAG CGAACGCAGG CCGCGGGGTA GGCCGTATAA GCCAAGGCTA 
CAGCTTCAAC AGCATCAACT GCAGCCGCAG CAGAAGTTAC AAGTGCAGCA GCATCAGCAG 
CAGCAGAAGG CACTTCAGCA ACTACCCCAA GCACAACACC ACCAACAGCA ACAGCAACCT 
CAACTTCAAC AAGAAGTGCT ACAGCAGGTG CAGCAACTGC AAGTGCAAGT GCAACCGCAG 
CAACACCAGC AACACCAACA AGTGTTGCAA GTGCAGCAAC CACAGCAACA ACCGTTAATG 
CAGCCACAGT CGCCGCATCC ATCAACAGTG GAAATGCAAG CAAGCCCAAC AACGCCACGA 
AAGATTCTTT GCTGTCCCGA TTGCGAAGAC TGCACTTCCG GCCACAGCCA TGCCAACGAA 
CAGTTCGAGG AGCTGCAGAC ACTGCAGGCG CCGCCAACGG TGTTGACTCC GCCATCGACC 
ATAGTCTCGG TACCATCGCC ACAGCCCATG GTGTACTCGC AGCACATAAC AATGCCGTCG 
CCGGAACAAT CCGAACCAGA TTCCACAACC ACGTTGCGAC AGTATCGCAA GCGAGGCGTG 
ATCGTTGGGC CACAGGGTCC GCTGCATTTG GCCACACCGG TGGCCTCGCC ATCGCCCTCG 
TCGTCACCCT CCTCATCGAC GGTGGATCAC ATACCGCCGG CATCTCCGGC TACGCCCGCA 
TCACCAGCTC CGCCTCCGTC GCCCGCCGTC GCGTCCACGG TGCAAGTGAG CGAGCTGCGA 
ACGAGTCACC ATTGTCTGTA CTGCGAGGAG CGATTCACCA ACGAAATTTC CCTGAAGAAG 
CACCATCAGC TGGCGCACGG AGCGCTGACA ACAATGCCAT ATGTGTGCAC AATCTGCAAG 
CGAGGGTATC GCATGCGTAC GGCGCTGCAT CGGCACATGG AGTCGCACGA TGTGGAGGGA 
CGGCCGTACG AGTGCAACAT TTGTCGCGTC CGGTTCCCGC GACCATCGCA ACTGACGCTG 
CACAAGATCA CGGTGCACCT GCTCTCCAAG CCGCACACCT GCGACGAGTG TGGCAAACAG 
TTTGGCACGG AGAGCGCCCT CAAGACGCAC ATTAAGTTCC ACGGAGAGCT CGGTTACCAG 
TGCGACGGGT GCGATCGGAC GTTCGAGTAT CTGAAAGAAT TGCGCAAACA TCGACGCACC 
CACAGCGAGA TGTTTTACAA ATGCAAATTC TGTCCAAGCT CCTTTATGCG TTTCACGAAT 
TTTCGGGCTC ACATGAAAAC CCATTTGCCG CTGGGCGTAT TCCGCAACGA GGATGCCGCA 
TCGAAGTCAC CCAGCAACAA CAATAGCCAC GCCAGCAGTG ACAAGCTGGA GAATCCAGCT 
ACGCCCATCG AGGAGACACC TTTGACGCCG ATGAGCAGTG GCGGCCACTT GTACCACAGC 
CCAGATGAAT ACCCCAATTC GGTGGAGAGC TGCGCTGGCA ACAGTGTGGC TCTGGAATCC 
TATGCCACAA CGCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mld ( NM_001260359.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001247288.1 Drosophila melanogaster molting defective (mld), transcript variant F, mRNA.

Price & Availability↑

CloneID	OFa08036
Clone ID Related Accession (Same CDS sequence)	NM_001260359.1
Accession Version	NM_001260359.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5691bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	molting defective, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGTGCCA ACCGAAGAAG ACGCAGTGCA TCAGCTGCGA GTTCAATTGC AGCAGAAACA 
GCAACAGCAG CTACAGCAGC AACAGCAGAA GCGATATCAA CTGCACCCTC CAAAGAGCAA 
CTTTCTCCAC CGATTGCTGG TGGCAGTGCT TCAGCTGATG AGGATTTACT GGAAATGCCT 
CGCATTTGCC GATGCTGTTG CAAACGGGAT TTGAAATTAC TCGGCTTATT CGAGGCCAGC 
CAACCAACAC TGGCCAACAC AACAGTGGCA TCAGCATCAG CAGCAGCATC AGTGGCATCG 
AACAAATCAT CGGGAACATT AGCAAAAACA TGTGCGACAG AAGCAGAAAC CGAAACTCCA 
CAGACATTGT CCGCAAATCC AGTAAATCCC GGGACTAAAG CTACATCATC TCCATCAGAA 
GCAGCTACAA CGTTGTCCAC ATCCACACGC CCCGCGTCCG CCTCATTCTC ATCCACCTCC 
TTTTCCACAT CCACGTCTCC CATGAGGCGT CGCACCACTA ACACCACCGC TCCATCCGCT 
TCGCCCGCTC CACGTGGTCG TAGCAGCGAT AATGCCGTCG ACTACACCCT CAGCGGGGCC 
CACAGCAGCA TGGACATTGT CCTCGAGGAG ATGACCATTT GGATGCTCAA TATAAATCGC 
GACGATGGAC TGCCGCAAGA AATCTGCCGG CAGTGCATGG CCCAGTTTTT GATGGTGGCC 
AAGTTCCGGC GGAAATGTGT ACGGATGCAG CAGCGCCTGC AGGACTTTGC CCAGGAGATA 
TCCGACCGCC AGACGGCCAG ACAAGCGAAG CGTCAGCAAA AAGTCCAAAA GAAGACACAC 
GATAGGTGGC TGCAGTTGGA GCCAACCAGC CACGAGGAGC AGCAGCCCAT ACGTAAGCGC 
CGCATGGTAT CCGAATCGGA CGGAGATTCG ACAGCAGCAG CAGCAGCAGC AGAAGCAGCA 
ACAGCAACAG CAACAACACC AGCCAAACAG GTGCGAACGG ATTTCGAGAG TTTGCCACCG 
CCCAAACCAA GTCCTCAACA GCACCGAGTG CGCAAGTATC GAACCCTCTC CATTGACTGT 
GAAATGGAGC GTCCCGCCGC TTCCATCAAT GCCAGCAGCA TGCCCTGGAA GACGAAGGCT 
GCCCGCAAAG AACTTTCGGA GAATTGGGCG TCCAGTTGCT CGACGGCGGT TATGTCGCCC 
AATCGCCAAC GCAGCAGCAC CAGCAGCAGT GAGCACATGA GCGACTTGTC CGTCGGTCCA 
TGCTGTGGCT TCGAAAGCGA CAGCAATCCG TCCTCTACCT CCAACTCGGC GCGGAGAACG 
GTGATACTCG TTAAGCAGCA ATCGCCGGAG AAGACATCGC CCATGTATGA TTTAATGCCG 
CGCAGAGGAC GGCGGCCCAG GAAAGCACCG ATATACATGC CGGCCAAAAG ACAGAGGCGA 
TCGCTGGTTA GAAAGGAGCC GCTATACCGA AAAGCACAGC CAGTCAACGA AGAGAACAAA 
AAGTCTGATC AAAAGGATAT ATATCCGAAG GAATCAAGGA TGGAAGGCCT TGCACCGTCC 
TGTGATGAGG CCATCGACAG CACTTCCGAT GCGCCAATGG AAGTAGCCTC TAATGTGGTC 
CAAAAGAAGT GCGATGAAAT CTGTTTAAAT GGTTTGATAG ACAGTCGCAA CCAGTTAGGC 
GCCATGGAAA TGGAGCCGGT TAATGAAATG ATAGCGTCCA ATGGTGTAGA TAAAGAACTA 
ACCCTCAGCG TTCAAAATAA TAAGGAAACA ACATCCGAAG AGCAAATATT TGAAGCCACC 
AGCGTTATAA AAGAATCAGT TCAACCAGAA CAATCTGCTG AAGATATTCA GGTTAGAACG 
GAAATCGAGT CAATTGCGAA TGACATCAGG GCTGAGGCTG ATCTAGTGCA GTCCGAGGAA 
CTGACCTCAT CCACAGAGAC CAGCGAGTCG CAACCGAAAG CAGTTGAGCA GCTGGAATCT 
ATGGGAAAGC AGGAATCGTA TGCGTTACCA CAAAACATGG AGGCGCATGA ATCCGTCCAA 
CAAACGCAAA CTGTGGACGA ATCAGACTCT GCTGACCAGA GGGAGATCAT AGTGAGCATA 
CCGCTGGAGG TTCTGGATGA GAATCTGCAG CGTCGCCTTT GCGTGGATCC GGAAACTGAG 
GCGGCCAATC GTCAGTCCGC TGATCCCAAG GAGTTTGCCC TGCGTCCCGA TGACGTGAAC 
AACAACGAAA ACGATGAAAA TTTTTGCCAG AGTGCTACAG CAGATAGCTT CAATTCTGCT 
GTGGCACTCC GAACCAATAA AATTGAAGCA ACACCGCCTG GGGGAACGGA TTCAGATGAC 
ACGGACACGG ATGAGGTGCC GATAGTGCCA TCTGGGCCAA CGCCCATGGA TTTCATGGCC 
GAGTTTGCCA AGCACTGTGC GAACGGAATT GAACAGCTCA AGGTTACTAC ACCGGTGCCA 
TCACCGACTC CCTCGGATTT GGTACCCCTC AACGATCTGG AGGCCAAGCA GAAACTGGAG 
GTGGAGATGA ACGACGTGGA GACCACACTC AATGGCATCC TCAATGAGAT GCAGGACCAG 
CACATGTACA CACCAAACTG TGCCCACATT GATGAGTTCC TCACTCCAGC CGATTATGCC 
ACACTGACTG AGCAGGAGTC GTCCGTTTCG TCGCCACCAA ATCCTTTCGA GCAGAGTTCT 
GTTGCCGAAC CGAAGGACGA AAAGCCCAGT GCCACTGAGG ATCCATTTGA TAATAGCAAC 
TTTACCAATG AACTGATTGG TTTTCAAAAC GATATTCCCT GTTTCGAGAA CATTGAGACA 
CCAGAGGAGG GACAAAATTT GCATTTAGAA CTGACCCAGT TCTTAAGGGA TCTGCAGCCG 
TCACAATCCG ATCCGCCGGC AGGGGAGCAG GTGGAAAAAA TCGAAATGGA GACACAGCCA 
AGCACCGTCC AAATGGATGT CCAATCAAAC CTACCAGTCC AAGCTGCTGA TACACAGCCT 
CAAAATGAGT CCGCTAATCA TTCACCATTT GAATCTCCCA TATCATCACC TCAAGTTCAG 
TCTCAGATCG AATTACAAAT GGAACCTCAA ATCGAGACTC AAATGCAACC TCAAATTGAG 
ACTCGAATGC AACACGATGT GCTAAGCGTG CAATCGCCAC AAAACCATCA GCCTCAAGTA 
CAATCTCATG ACCAATCTCA GATGCAACAC CAAATCCCAC CGCAAATTGA GCCTCAAATT 
GAAGTCCAAA ACCAGGAGCA AAATACTTTA CAGCTCGTGC CGCAAGCACA GCATCAAGTG 
GTTCCACATG TACAACTGCA GTCCCAGTTG GTGCCTCACG TCCAGACGCA GGGCACGACG 
ACGACAGTCA CCTACGAACA GATTTACCAG CAGCACATTG TCCAGCAGAC GGTGCAGCAA 
TCCTCCAACA AGATGCAGGT CATCTCGCTG CCCTCGGCTG GCTCGGAAAC AACACGACAG 
TGGCAGACGC AGCAGTCGCA GCAACAGCAG CAAGTCCAGT GGTCATCGGT TGGCGGACTT 
GAGGCCATAA AGAGTGCACC CGTCGAGAGT GTTGCACCCA CGACCACAAC GTACTACATC 
AGTGCGAGCG ATCTTTATCC GCAGCAGACT GAAACATACA CGATGCTTCA ACCAGCATCC 
AATGAAAGCT ACGTCATTGA GCAGGTGAAT CATCAGCAGC AACATTGCCA GCAACAGATT 
CAGATGTCGC AACAGCAACA TCAACGCCAG CATCAGCAAC AGCAGGCGCA ACAGCAGCAG 
CAACCGATTA TGATACTGAT CCAGCAGCCA GAGATTCAGC AGCCAGTGGC CTCGGCCAGC 
AATCCGCAGC AGGCACCGCT GCACATCTAT AATGCTGCCA CCAGCAACGA TGTGCATCAA 
ATGCAGCACA CGCAACGCCA ACAGATACGG CAGCAGTATC AACACCAGCA GTATCAGCGG 
CTGCAACAGC CGCAACAAAC TCGCGTCGTA CAAAGCCATC CAGTATTGGG AATGCCGGGA 
GCCACTTCCA TATCAACGAA AGTGACCCCC ATTCCCGTCA CAGCGGCCAG GGGTCGGCCG 
CTCACGCTGA AGTGCCGCTT CTGCCATAAC GGGCCGCGAT TCTCCAGCAG CCTGGAGTAC 
AGCCGGCATA TCATCGATCT GCATCCGGCG GTGGCGCCCT TCAATTGTCC ACACTGTCCG 
ATGGCCTTTG CCGGCCGAAC GAAGCGATCG CAGCACATCC TCAGCCATCA TGTGGTGCAG 
CAGTACCAGT GCGGTCAGTG CTCACATGTG TTTCCCGCCC AGAAGGCACT GGACGTTCAC 
ATCCAGCGCT TTCATATGAC CCTGAAAACG GATCCATCGG GCGCTGTGAA GGTGGAGGAC 
GTTCAGCTGC AGATCACCAG CGAACGCAGG CCGCGGGGTA GGCCGTATAA GCCAAGGCTA 
CAGCTTCAAC AGCATCAACT GCAGCCGCAG CAGAAGTTAC AAGTGCAGCA GCATCAGCAG 
CAGCAGAAGG CACTTCAGCA ACTACCCCAA GCACAACACC ACCAACAGCA ACAGCAACCT 
CAACTTCAAC AAGAAGTGCT ACAGCAGGTG CAGCAACTGC AAGTGCAAGT GCAACCGCAG 
CAACACCAGC AACACCAACA AGTGTTGCAA GTGCAGCAAC CACAGCAACA ACCGTTAATG 
CAGCCACAGT CGCCGCATCC ATCAAGCACT TCCGGCCACA GCCATGCCAA CGAACAGTTC 
GAGGAGCTGC AGACACTGCA GGCGCCGCCA ACGGTGTTGA CTCCGCCATC GACCATAGTC 
TCGGTACCAT CGCCACAGCC CATGGTGTAC TCGCAGCACA TAACAATGCC GTCGCCGGAA 
CAATCCGAAC CAGATTCCAC AACCACGTTG CGACAGTATC GCAAGCGAGG CGTGATCGTT 
GGGCCACAGG GTCCGCTGCA TTTGGCCACA CCGGTGGCCT CGCCATCGCC CTCGTCGTCA 
CCCTCCTCAT CGACGGTGGA TCACATACCG CCGGCATCTC CGGCTACGCC CGCATCACCA 
GCTCCGCCTC CGTCGCCCGC CGTCGCGTCC ACGGTGCAAG TGAGCGAGCT GCGAACGAGT 
CACCATTGTC TGTACTGCGA GGAGCGATTC ACCAACGAAA TTTCCCTGAA GAAGCACCAT 
CAGCTGGCGC ACGGAGCGCT GACAACAATG CCATATGTGT GCACAATCTG CAAGCGAGGG 
TATCGCATGC GTACGGCGCT GCATCGGCAC ATGGAGTCGC ACGATGTGGA GGGACGGCCG 
TACGAGTGCA ACATTTGTCG CGTCCGGTTC CCGCGACCAT CGCAACTGAC GCTGCACAAG 
ATCACGGTGC ACCTGCTCTC CAAGCCGCAC ACCTGCGACG AGTGTGGCAA ACAGTTTGGC 
ACGGAGAGCG CCCTCAAGAC GCACATTAAG TTCCACGGAG CTCACATGAA AACCCATTTG 
CCGCTGGGCG TATTCCGCAA CGAGGATGCC GCATCGAAGT CACCCAGCAA CAACAATAGC 
CACGCCAGCA GTGACAAGCT GGAGAATCCA GCTACGCCCA TCGAGGAGAC ACCTTTGACG 
CCGATGAGCA GTGGCGGCCA CTTGTACCAC AGCCCAGATG AATACCCCAA TTCGGTGGAG 
AGCTGCGCTG GCAACAGTGT GGCTCTGGAA TCCTATGCCA CAACGCCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247288.1
CDS	474..6164
Translation	MSANRRRRSASAASSIAAETATAATAATAEAISTAPSKEQLSPPIAGGSASADEDLLEMPRICRCCCKRDLKLLGLFEASQPTLANTTVASASAAASVASNKSSGTLAKTCATEAETETPQTLSANPVNPGTKATSSPSEAATTLSTSTRPASASFSSTSFSTSTSPMRRRTTNTTAPSASPAPRGRSSDNAVDYTLSGAHSSMDIVLEEMTIWMLNINRDDGLPQEICRQCMAQFLMVAKFRRKCVRMQQRLQDFAQEISDRQTARQAKRQQKVQKKTHDRWLQLEPTSHEEQQPIRKRRMVSESDGDSTAAAAAAEAATATATTPAKQVRTDFESLPPPKPSPQQHRVRKYRTLSIDCEMERPAASINASSMPWKTKAARKELSENWASSCSTAVMSPNRQRSSTSSSEHMSDLSVGPCCGFESDSNPSSTSNSARRTVILVKQQSPEKTSPMYDLMPRRGRRPRKAPIYMPAKRQRRSLVRKEPLYRKAQPVNEENKKSDQKDIYPKESRMEGLAPSCDEAIDSTSDAPMEVASNVVQKKCDEICLNGLIDSRNQLGAMEMEPVNEMIASNGVDKELTLSVQNNKETTSEEQIFEATSVIKESVQPEQSAEDIQVRTEIESIANDIRAEADLVQSEELTSSTETSESQPKAVEQLESMGKQESYALPQNMEAHESVQQTQTVDESDSADQREIIVSIPLEVLDENLQRRLCVDPETEAANRQSADPKEFALRPDDVNNNENDENFCQSATADSFNSAVALRTNKIEATPPGGTDSDDTDTDEVPIVPSGPTPMDFMAEFAKHCANGIEQLKVTTPVPSPTPSDLVPLNDLEAKQKLEVEMNDVETTLNGILNEMQDQHMYTPNCAHIDEFLTPADYATLTEQESSVSSPPNPFEQSSVAEPKDEKPSATEDPFDNSNFTNELIGFQNDIPCFENIETPEEGQNLHLELTQFLRDLQPSQSDPPAGEQVEKIEMETQPSTVQMDVQSNLPVQAADTQPQNESANHSPFESPISSPQVQSQIELQMEPQIETQMQPQIETRMQHDVLSVQSPQNHQPQVQSHDQSQMQHQIPPQIEPQIEVQNQEQNTLQLVPQAQHQVVPHVQLQSQLVPHVQTQGTTTTVTYEQIYQQHIVQQTVQQSSNKMQVISLPSAGSETTRQWQTQQSQQQQQVQWSSVGGLEAIKSAPVESVAPTTTTYYISASDLYPQQTETYTMLQPASNESYVIEQVNHQQQHCQQQIQMSQQQHQRQHQQQQAQQQQQPIMILIQQPEIQQPVASASNPQQAPLHIYNAATSNDVHQMQHTQRQQIRQQYQHQQYQRLQQPQQTRVVQSHPVLGMPGATSISTKVTPIPVTAARGRPLTLKCRFCHNGPRFSSSLEYSRHIIDLHPAVAPFNCPHCPMAFAGRTKRSQHILSHHVVQQYQCGQCSHVFPAQKALDVHIQRFHMTLKTDPSGAVKVEDVQLQITSERRPRGRPYKPRLQLQQHQLQPQQKLQVQQHQQQQKALQQLPQAQHHQQQQQPQLQQEVLQQVQQLQVQVQPQQHQQHQQVLQVQQPQQQPLMQPQSPHPSSTSGHSHANEQFEELQTLQAPPTVLTPPSTIVSVPSPQPMVYSQHITMPSPEQSEPDSTTTLRQYRKRGVIVGPQGPLHLATPVASPSPSSSPSSSTVDHIPPASPATPASPAPPPSPAVASTVQVSELRTSHHCLYCEERFTNEISLKKHHQLAHGALTTMPYVCTICKRGYRMRTALHRHMESHDVEGRPYECNICRVRFPRPSQLTLHKITVHLLSKPHTCDECGKQFGTESALKTHIKFHGAHMKTHLPLGVFRNEDAASKSPSNNNSHASSDKLENPATPIEETPLTPMSSGGHLYHSPDEYPNSVESCAGNSVALESYATTP

Target ORF information:

RefSeq Version	NM_001260359.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster molting defective (mld), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260359.1

ORF Insert Sequence:

ATGAGTGCCA ACCGAAGAAG ACGCAGTGCA TCAGCTGCGA GTTCAATTGC AGCAGAAACA 
GCAACAGCAG CTACAGCAGC AACAGCAGAA GCGATATCAA CTGCACCCTC CAAAGAGCAA 
CTTTCTCCAC CGATTGCTGG TGGCAGTGCT TCAGCTGATG AGGATTTACT GGAAATGCCT 
CGCATTTGCC GATGCTGTTG CAAACGGGAT TTGAAATTAC TCGGCTTATT CGAGGCCAGC 
CAACCAACAC TGGCCAACAC AACAGTGGCA TCAGCATCAG CAGCAGCATC AGTGGCATCG 
AACAAATCAT CGGGAACATT AGCAAAAACA TGTGCGACAG AAGCAGAAAC CGAAACTCCA 
CAGACATTGT CCGCAAATCC AGTAAATCCC GGGACTAAAG CTACATCATC TCCATCAGAA 
GCAGCTACAA CGTTGTCCAC ATCCACACGC CCCGCGTCCG CCTCATTCTC ATCCACCTCC 
TTTTCCACAT CCACGTCTCC CATGAGGCGT CGCACCACTA ACACCACCGC TCCATCCGCT 
TCGCCCGCTC CACGTGGTCG TAGCAGCGAT AATGCCGTCG ACTACACCCT CAGCGGGGCC 
CACAGCAGCA TGGACATTGT CCTCGAGGAG ATGACCATTT GGATGCTCAA TATAAATCGC 
GACGATGGAC TGCCGCAAGA AATCTGCCGG CAGTGCATGG CCCAGTTTTT GATGGTGGCC 
AAGTTCCGGC GGAAATGTGT ACGGATGCAG CAGCGCCTGC AGGACTTTGC CCAGGAGATA 
TCCGACCGCC AGACGGCCAG ACAAGCGAAG CGTCAGCAAA AAGTCCAAAA GAAGACACAC 
GATAGGTGGC TGCAGTTGGA GCCAACCAGC CACGAGGAGC AGCAGCCCAT ACGTAAGCGC 
CGCATGGTAT CCGAATCGGA CGGAGATTCG ACAGCAGCAG CAGCAGCAGC AGAAGCAGCA 
ACAGCAACAG CAACAACACC AGCCAAACAG GTGCGAACGG ATTTCGAGAG TTTGCCACCG 
CCCAAACCAA GTCCTCAACA GCACCGAGTG CGCAAGTATC GAACCCTCTC CATTGACTGT 
GAAATGGAGC GTCCCGCCGC TTCCATCAAT GCCAGCAGCA TGCCCTGGAA GACGAAGGCT 
GCCCGCAAAG AACTTTCGGA GAATTGGGCG TCCAGTTGCT CGACGGCGGT TATGTCGCCC 
AATCGCCAAC GCAGCAGCAC CAGCAGCAGT GAGCACATGA GCGACTTGTC CGTCGGTCCA 
TGCTGTGGCT TCGAAAGCGA CAGCAATCCG TCCTCTACCT CCAACTCGGC GCGGAGAACG 
GTGATACTCG TTAAGCAGCA ATCGCCGGAG AAGACATCGC CCATGTATGA TTTAATGCCG 
CGCAGAGGAC GGCGGCCCAG GAAAGCACCG ATATACATGC CGGCCAAAAG ACAGAGGCGA 
TCGCTGGTTA GAAAGGAGCC GCTATACCGA AAAGCACAGC CAGTCAACGA AGAGAACAAA 
AAGTCTGATC AAAAGGATAT ATATCCGAAG GAATCAAGGA TGGAAGGCCT TGCACCGTCC 
TGTGATGAGG CCATCGACAG CACTTCCGAT GCGCCAATGG AAGTAGCCTC TAATGTGGTC 
CAAAAGAAGT GCGATGAAAT CTGTTTAAAT GGTTTGATAG ACAGTCGCAA CCAGTTAGGC 
GCCATGGAAA TGGAGCCGGT TAATGAAATG ATAGCGTCCA ATGGTGTAGA TAAAGAACTA 
ACCCTCAGCG TTCAAAATAA TAAGGAAACA ACATCCGAAG AGCAAATATT TGAAGCCACC 
AGCGTTATAA AAGAATCAGT TCAACCAGAA CAATCTGCTG AAGATATTCA GGTTAGAACG 
GAAATCGAGT CAATTGCGAA TGACATCAGG GCTGAGGCTG ATCTAGTGCA GTCCGAGGAA 
CTGACCTCAT CCACAGAGAC CAGCGAGTCG CAACCGAAAG CAGTTGAGCA GCTGGAATCT 
ATGGGAAAGC AGGAATCGTA TGCGTTACCA CAAAACATGG AGGCGCATGA ATCCGTCCAA 
CAAACGCAAA CTGTGGACGA ATCAGACTCT GCTGACCAGA GGGAGATCAT AGTGAGCATA 
CCGCTGGAGG TTCTGGATGA GAATCTGCAG CGTCGCCTTT GCGTGGATCC GGAAACTGAG 
GCGGCCAATC GTCAGTCCGC TGATCCCAAG GAGTTTGCCC TGCGTCCCGA TGACGTGAAC 
AACAACGAAA ACGATGAAAA TTTTTGCCAG AGTGCTACAG CAGATAGCTT CAATTCTGCT 
GTGGCACTCC GAACCAATAA AATTGAAGCA ACACCGCCTG GGGGAACGGA TTCAGATGAC 
ACGGACACGG ATGAGGTGCC GATAGTGCCA TCTGGGCCAA CGCCCATGGA TTTCATGGCC 
GAGTTTGCCA AGCACTGTGC GAACGGAATT GAACAGCTCA AGGTTACTAC ACCGGTGCCA 
TCACCGACTC CCTCGGATTT GGTACCCCTC AACGATCTGG AGGCCAAGCA GAAACTGGAG 
GTGGAGATGA ACGACGTGGA GACCACACTC AATGGCATCC TCAATGAGAT GCAGGACCAG 
CACATGTACA CACCAAACTG TGCCCACATT GATGAGTTCC TCACTCCAGC CGATTATGCC 
ACACTGACTG AGCAGGAGTC GTCCGTTTCG TCGCCACCAA ATCCTTTCGA GCAGAGTTCT 
GTTGCCGAAC CGAAGGACGA AAAGCCCAGT GCCACTGAGG ATCCATTTGA TAATAGCAAC 
TTTACCAATG AACTGATTGG TTTTCAAAAC GATATTCCCT GTTTCGAGAA CATTGAGACA 
CCAGAGGAGG GACAAAATTT GCATTTAGAA CTGACCCAGT TCTTAAGGGA TCTGCAGCCG 
TCACAATCCG ATCCGCCGGC AGGGGAGCAG GTGGAAAAAA TCGAAATGGA GACACAGCCA 
AGCACCGTCC AAATGGATGT CCAATCAAAC CTACCAGTCC AAGCTGCTGA TACACAGCCT 
CAAAATGAGT CCGCTAATCA TTCACCATTT GAATCTCCCA TATCATCACC TCAAGTTCAG 
TCTCAGATCG AATTACAAAT GGAACCTCAA ATCGAGACTC AAATGCAACC TCAAATTGAG 
ACTCGAATGC AACACGATGT GCTAAGCGTG CAATCGCCAC AAAACCATCA GCCTCAAGTA 
CAATCTCATG ACCAATCTCA GATGCAACAC CAAATCCCAC CGCAAATTGA GCCTCAAATT 
GAAGTCCAAA ACCAGGAGCA AAATACTTTA CAGCTCGTGC CGCAAGCACA GCATCAAGTG 
GTTCCACATG TACAACTGCA GTCCCAGTTG GTGCCTCACG TCCAGACGCA GGGCACGACG 
ACGACAGTCA CCTACGAACA GATTTACCAG CAGCACATTG TCCAGCAGAC GGTGCAGCAA 
TCCTCCAACA AGATGCAGGT CATCTCGCTG CCCTCGGCTG GCTCGGAAAC AACACGACAG 
TGGCAGACGC AGCAGTCGCA GCAACAGCAG CAAGTCCAGT GGTCATCGGT TGGCGGACTT 
GAGGCCATAA AGAGTGCACC CGTCGAGAGT GTTGCACCCA CGACCACAAC GTACTACATC 
AGTGCGAGCG ATCTTTATCC GCAGCAGACT GAAACATACA CGATGCTTCA ACCAGCATCC 
AATGAAAGCT ACGTCATTGA GCAGGTGAAT CATCAGCAGC AACATTGCCA GCAACAGATT 
CAGATGTCGC AACAGCAACA TCAACGCCAG CATCAGCAAC AGCAGGCGCA ACAGCAGCAG 
CAACCGATTA TGATACTGAT CCAGCAGCCA GAGATTCAGC AGCCAGTGGC CTCGGCCAGC 
AATCCGCAGC AGGCACCGCT GCACATCTAT AATGCTGCCA CCAGCAACGA TGTGCATCAA 
ATGCAGCACA CGCAACGCCA ACAGATACGG CAGCAGTATC AACACCAGCA GTATCAGCGG 
CTGCAACAGC CGCAACAAAC TCGCGTCGTA CAAAGCCATC CAGTATTGGG AATGCCGGGA 
GCCACTTCCA TATCAACGAA AGTGACCCCC ATTCCCGTCA CAGCGGCCAG GGGTCGGCCG 
CTCACGCTGA AGTGCCGCTT CTGCCATAAC GGGCCGCGAT TCTCCAGCAG CCTGGAGTAC 
AGCCGGCATA TCATCGATCT GCATCCGGCG GTGGCGCCCT TCAATTGTCC ACACTGTCCG 
ATGGCCTTTG CCGGCCGAAC GAAGCGATCG CAGCACATCC TCAGCCATCA TGTGGTGCAG 
CAGTACCAGT GCGGTCAGTG CTCACATGTG TTTCCCGCCC AGAAGGCACT GGACGTTCAC 
ATCCAGCGCT TTCATATGAC CCTGAAAACG GATCCATCGG GCGCTGTGAA GGTGGAGGAC 
GTTCAGCTGC AGATCACCAG CGAACGCAGG CCGCGGGGTA GGCCGTATAA GCCAAGGCTA 
CAGCTTCAAC AGCATCAACT GCAGCCGCAG CAGAAGTTAC AAGTGCAGCA GCATCAGCAG 
CAGCAGAAGG CACTTCAGCA ACTACCCCAA GCACAACACC ACCAACAGCA ACAGCAACCT 
CAACTTCAAC AAGAAGTGCT ACAGCAGGTG CAGCAACTGC AAGTGCAAGT GCAACCGCAG 
CAACACCAGC AACACCAACA AGTGTTGCAA GTGCAGCAAC CACAGCAACA ACCGTTAATG 
CAGCCACAGT CGCCGCATCC ATCAAGCACT TCCGGCCACA GCCATGCCAA CGAACAGTTC 
GAGGAGCTGC AGACACTGCA GGCGCCGCCA ACGGTGTTGA CTCCGCCATC GACCATAGTC 
TCGGTACCAT CGCCACAGCC CATGGTGTAC TCGCAGCACA TAACAATGCC GTCGCCGGAA 
CAATCCGAAC CAGATTCCAC AACCACGTTG CGACAGTATC GCAAGCGAGG CGTGATCGTT 
GGGCCACAGG GTCCGCTGCA TTTGGCCACA CCGGTGGCCT CGCCATCGCC CTCGTCGTCA 
CCCTCCTCAT CGACGGTGGA TCACATACCG CCGGCATCTC CGGCTACGCC CGCATCACCA 
GCTCCGCCTC CGTCGCCCGC CGTCGCGTCC ACGGTGCAAG TGAGCGAGCT GCGAACGAGT 
CACCATTGTC TGTACTGCGA GGAGCGATTC ACCAACGAAA TTTCCCTGAA GAAGCACCAT 
CAGCTGGCGC ACGGAGCGCT GACAACAATG CCATATGTGT GCACAATCTG CAAGCGAGGG 
TATCGCATGC GTACGGCGCT GCATCGGCAC ATGGAGTCGC ACGATGTGGA GGGACGGCCG 
TACGAGTGCA ACATTTGTCG CGTCCGGTTC CCGCGACCAT CGCAACTGAC GCTGCACAAG 
ATCACGGTGC ACCTGCTCTC CAAGCCGCAC ACCTGCGACG AGTGTGGCAA ACAGTTTGGC 
ACGGAGAGCG CCCTCAAGAC GCACATTAAG TTCCACGGAG CTCACATGAA AACCCATTTG 
CCGCTGGGCG TATTCCGCAA CGAGGATGCC GCATCGAAGT CACCCAGCAA CAACAATAGC 
CACGCCAGCA GTGACAAGCT GGAGAATCCA GCTACGCCCA TCGAGGAGAC ACCTTTGACG 
CCGATGAGCA GTGGCGGCCA CTTGTACCAC AGCCCAGATG AATACCCCAA TTCGGTGGAG 
AGCTGCGCTG GCAACAGTGT GGCTCTGGAA TCCTATGCCA CAACGCCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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mld cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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