Pgant4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pgant4
Entrez Gene ID	261610
Full Name	polypeptide GalNAc transferase 4
Synonyms	CG31956,CG8845,CG8845b,Dmel\CG31956
General protein information	Preferred Names polypeptide GalNAc transferase 4 Names CG31956 gene product from transcript CG31956-RC CG31956-PB CG31956-PC pgant4-PB pgant4-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 23F3-23F3

mRNA and Protein(s)

mRNA	Protein	Name
NM_164539.2	NP_722910.2	polypeptide N-Acetylgalactosaminyltransferase 4, isoform C
NM_001144307.2	NP_001137779.1	polypeptide N-Acetylgalactosaminyltransferase 4, isoform B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PGANT4 is an O-glycosyltransferase that functions as a regulator of secretion and secretory vesicle formation in vivo by glycosylating the essential Golgi/endoplasmic reticulum protein, Tango1 and conferring protection from furin-mediated proteolysis
Title: O-glycosylation regulates polarized secretion by modulating Tango1 stability.

The following Pgant4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pgant4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa15393	NM_001144307.2 Latest version!	Drosophila melanogaster polypeptide N-Acetylgalactosaminyltransferase 4, transcript variant B (Pgant4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa15393	NM_164539.2 Latest version!	Drosophila melanogaster polypeptide N-Acetylgalactosaminyltransferase 4, transcript variant C (Pgant4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pgant4 ( NM_164539.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_722910.2 Drosophila melanogaster polypeptide N-Acetylgalactosaminyltransferase 4, transcript variant C (Pgant4), mRNA.

Price & Availability↑

CloneID	OFa15393
Clone ID Related Accession (Same CDS sequence)	NM_164539.2 , NM_001144307.2
Accession Version	NM_164539.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	polypeptide N-Acetylgalactosaminyltransferase 4, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_164539.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCATAA AAAAGAGATA TGTAAAGCGC CTGTTGCGCA AGGTGGTCCT CCTCTTGGTG 
GTCATCGTCA CTGTGTCCTT GGTCACCACT TTGGTGGTCG AACGGCGAAT GAAAAATGCG 
GCGGAGCTGA CGGAACAACT CGATCCGAAT GGAGATCCCA TAACGCCCGT TTTTAGGGCT 
GCCAATATAC ATCCAACTCG GAAAGCGCCA CGACCACCAT TTCAGGATCG CAACTCCGTG 
GTAGATATTC CGAGAAGTGA CAAACTCCAG GGATTCCGAC TACCGGAACC GAAGGGAGAG 
CGCAAGGATT GGCACGACTA TGCGGCCATG GAGGCGGATA GAAAGCGATC CGGTTTCGGT 
GAGCACGGAG TAGCCGTAAA GATCGAAAAT CCCGATGAGA AGCAGTTGGA AAAGGAGCAC 
TACGAGATGA ACGGCTTTAA TGGCCTCATA TCGGATAGAA TCTCCGTCAA TCGATCTGTG 
CCAGACCTCA GACTCGAGGC TTGCAAGACC CGGAAGTATC TGGCCAAGTT GCCCAATATC 
AGCGTGATTT TCATCTTCTT CAACGAGCAC TTTAACACCC TGTTGAGATC GATATACAGT 
GTGATAAATC GCACTCCACC GGAATTACTT AAGCAAATTG TGCTCGTGGA CGATGGCAGC 
GAGTGGGATG TTCTGAAGCA GCCTTTGGAC GACTACGTCC AGCAGCACTT TCCACATCTG 
GTGACTATAG TGCGCAATCC GGAGAGACAA GGTCTAATTG GAGCCAGAAT AGCTGGAGCC 
AAGGTGGCAG TGGGACAAGT GATGGTGTTC TTTGATTCTC ACATAGAAGT CAACTACAAT 
TGGCTGCCTC CCCTGATCGA ACCCATTGCC ATTAATCCCA AGATCTCCAC CTGCCCCATG 
GTGGATACTA TTTCGCATGA AGATTTCTCT TACTTTAGCG GAAATAAGGA CGGAGCACGA 
GGTGGATTCG ATTGGAAGAT GCTGTACAAA CAGCTTCCGG TTCTGCCCGA AGATGCGCTG 
GACAAGTCCA TGCCGTACCG GAGTCCCGTG ATGATGGGCG GTCTATTTGC CATCAACACG 
GACTTCTTTT GGGATCTGGG CGGATACGAC GACCAGTTGG ACATCTGGGG CGGTGAGCAG 
TACGAGCTGA GTTTCAAGAT CTGGATGTGT GGCGGTATGC TGCTGGATGT TCCCTGCTCG 
AGGGTGGCAC ACATATTCCG GGGACCCATG AAGCCAAGGG GCAATCCAAG GGGACACAAC 
TTTGTGGCCA AGAACCACAA GCGCGTGGCA GAGGTTTGGA TGGATGAGTA TAAGCAATAT 
GTGTACAAAC GCGATCCGAA GACCTATGAC AACCTGGATG CCGGAGATCT GACCCGCCAG 
CGTGGAGTTC GAGAGCGACT CAAGTGCAAG AGTTTCCACT GGTTCATGAC GGAGGTGGCG 
CCTGATTTCC TCGTCAAATT CCCGCCCGTG GAACCACCTA GCTACGCCGC CGGCATCATT 
CAGAACGTGG CCAACCCGGT CTATTGCCTG GACAACATGG GCAAGTCCAC CGAGGAGGCG 
GTGGGTATGT TCAGCTGTGC GGACAATAGG ACCCATCCGC AACCGAATCA GTTTTGGGAG 
CTCTCCATAT TCCGAGACCT CCGGATGAAG GGCTTCGATT CAGTGTGCCT GGATGTGCAC 
GAGGGACCGC CGAATGCCAC CGTTTGGATG TGGAGCTGCC ACAGTCAGGG TGGCAATCAG 
TTCTGGTACT ATGACAGGCA AACCCAAAGG TTGGTTCACG GAGAGAACAA CAAGCGCTGC 
CTGGAGGGAT TTGTGGAGAA CGGCATAGCC AAGGTGGTGG CCAATTCCTG CGAGAATGGC 
AACGATCGCC AGCGCTGGGA GTTCGGATTC GTGAACCACA CTATGCTGGA CACCTTCTAC 
GATGGATTAA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_722910.2
CDS	201..2135
Translation	MAIKKRYVKRLLRKVVLLLVVIVTVSLVTTLVVERRMKNAAELTEQLDPNGDPITPVFRAANIHPTRKAPRPPFQDRNSVVDIPRSDKLQGFRLPEPKGERKDWHDYAAMEADRKRSGFGEHGVAVKIENPDEKQLEKEHYEMNGFNGLISDRISVNRSVPDLRLEACKTRKYLAKLPNISVIFIFFNEHFNTLLRSIYSVINRTPPELLKQIVLVDDGSEWDVLKQPLDDYVQQHFPHLVTIVRNPERQGLIGARIAGAKVAVGQVMVFFDSHIEVNYNWLPPLIEPIAINPKISTCPMVDTISHEDFSYFSGNKDGARGGFDWKMLYKQLPVLPEDALDKSMPYRSPVMMGGLFAINTDFFWDLGGYDDQLDIWGGEQYELSFKIWMCGGMLLDVPCSRVAHIFRGPMKPRGNPRGHNFVAKNHKRVAEVWMDEYKQYVYKRDPKTYDNLDAGDLTRQRGVRERLKCKSFHWFMTEVAPDFLVKFPPVEPPSYAAGIIQNVANPVYCLDNMGKSTEEAVGMFSCADNRTHPQPNQFWELSIFRDLRMKGFDSVCLDVHEGPPNATVWMWSCHSQGGNQFWYYDRQTQRLVHGENNKRCLEGFVENGIAKVVANSCENGNDRQRWEFGFVNHTMLDTFYDGLK

Target ORF information:

RefSeq Version	NM_164539.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster polypeptide N-Acetylgalactosaminyltransferase 4, transcript variant C (Pgant4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_164539.2

ORF Insert Sequence:

ATGGCCATAA AAAAGAGATA TGTAAAGCGC CTGTTGCGCA AGGTGGTCCT CCTCTTGGTG 
GTCATCGTCA CTGTGTCCTT GGTCACCACT TTGGTGGTCG AACGGCGAAT GAAAAATGCG 
GCGGAGCTGA CGGAACAACT CGATCCGAAT GGAGATCCCA TAACGCCCGT TTTTAGGGCT 
GCCAATATAC ATCCAACTCG GAAAGCGCCA CGACCACCAT TTCAGGATCG CAACTCCGTG 
GTAGATATTC CGAGAAGTGA CAAACTCCAG GGATTCCGAC TACCGGAACC GAAGGGAGAG 
CGCAAGGATT GGCACGACTA TGCGGCCATG GAGGCGGATA GAAAGCGATC CGGTTTCGGT 
GAGCACGGAG TAGCCGTAAA GATCGAAAAT CCCGATGAGA AGCAGTTGGA AAAGGAGCAC 
TACGAGATGA ACGGCTTTAA TGGCCTCATA TCGGATAGAA TCTCCGTCAA TCGATCTGTG 
CCAGACCTCA GACTCGAGGC TTGCAAGACC CGGAAGTATC TGGCCAAGTT GCCCAATATC 
AGCGTGATTT TCATCTTCTT CAACGAGCAC TTTAACACCC TGTTGAGATC GATATACAGT 
GTGATAAATC GCACTCCACC GGAATTACTT AAGCAAATTG TGCTCGTGGA CGATGGCAGC 
GAGTGGGATG TTCTGAAGCA GCCTTTGGAC GACTACGTCC AGCAGCACTT TCCACATCTG 
GTGACTATAG TGCGCAATCC GGAGAGACAA GGTCTAATTG GAGCCAGAAT AGCTGGAGCC 
AAGGTGGCAG TGGGACAAGT GATGGTGTTC TTTGATTCTC ACATAGAAGT CAACTACAAT 
TGGCTGCCTC CCCTGATCGA ACCCATTGCC ATTAATCCCA AGATCTCCAC CTGCCCCATG 
GTGGATACTA TTTCGCATGA AGATTTCTCT TACTTTAGCG GAAATAAGGA CGGAGCACGA 
GGTGGATTCG ATTGGAAGAT GCTGTACAAA CAGCTTCCGG TTCTGCCCGA AGATGCGCTG 
GACAAGTCCA TGCCGTACCG GAGTCCCGTG ATGATGGGCG GTCTATTTGC CATCAACACG 
GACTTCTTTT GGGATCTGGG CGGATACGAC GACCAGTTGG ACATCTGGGG CGGTGAGCAG 
TACGAGCTGA GTTTCAAGAT CTGGATGTGT GGCGGTATGC TGCTGGATGT TCCCTGCTCG 
AGGGTGGCAC ACATATTCCG GGGACCCATG AAGCCAAGGG GCAATCCAAG GGGACACAAC 
TTTGTGGCCA AGAACCACAA GCGCGTGGCA GAGGTTTGGA TGGATGAGTA TAAGCAATAT 
GTGTACAAAC GCGATCCGAA GACCTATGAC AACCTGGATG CCGGAGATCT GACCCGCCAG 
CGTGGAGTTC GAGAGCGACT CAAGTGCAAG AGTTTCCACT GGTTCATGAC GGAGGTGGCG 
CCTGATTTCC TCGTCAAATT CCCGCCCGTG GAACCACCTA GCTACGCCGC CGGCATCATT 
CAGAACGTGG CCAACCCGGT CTATTGCCTG GACAACATGG GCAAGTCCAC CGAGGAGGCG 
GTGGGTATGT TCAGCTGTGC GGACAATAGG ACCCATCCGC AACCGAATCA GTTTTGGGAG 
CTCTCCATAT TCCGAGACCT CCGGATGAAG GGCTTCGATT CAGTGTGCCT GGATGTGCAC 
GAGGGACCGC CGAATGCCAC CGTTTGGATG TGGAGCTGCC ACAGTCAGGG TGGCAATCAG 
TTCTGGTACT ATGACAGGCA AACCCAAAGG TTGGTTCACG GAGAGAACAA CAAGCGCTGC 
CTGGAGGGAT TTGTGGAGAA CGGCATAGCC AAGGTGGTGG CCAATTCCTG CGAGAATGGC 
AACGATCGCC AGCGCTGGGA GTTCGGATTC GTGAACCACA CTATGCTGGA CACCTTCTAC 
GATGGATTAA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pgant4 ( NM_001144307.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137779.1 Drosophila melanogaster polypeptide N-Acetylgalactosaminyltransferase 4, transcript variant B (Pgant4), mRNA.

Price & Availability↑

CloneID	OFa15393
Clone ID Related Accession (Same CDS sequence)	NM_164539.2 , NM_001144307.2
Accession Version	NM_001144307.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	polypeptide N-Acetylgalactosaminyltransferase 4, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_001144307.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCATAA AAAAGAGATA TGTAAAGCGC CTGTTGCGCA AGGTGGTCCT CCTCTTGGTG 
GTCATCGTCA CTGTGTCCTT GGTCACCACT TTGGTGGTCG AACGGCGAAT GAAAAATGCG 
GCGGAGCTGA CGGAACAACT CGATCCGAAT GGAGATCCCA TAACGCCCGT TTTTAGGGCT 
GCCAATATAC ATCCAACTCG GAAAGCGCCA CGACCACCAT TTCAGGATCG CAACTCCGTG 
GTAGATATTC CGAGAAGTGA CAAACTCCAG GGATTCCGAC TACCGGAACC GAAGGGAGAG 
CGCAAGGATT GGCACGACTA TGCGGCCATG GAGGCGGATA GAAAGCGATC CGGTTTCGGT 
GAGCACGGAG TAGCCGTAAA GATCGAAAAT CCCGATGAGA AGCAGTTGGA AAAGGAGCAC 
TACGAGATGA ACGGCTTTAA TGGCCTCATA TCGGATAGAA TCTCCGTCAA TCGATCTGTG 
CCAGACCTCA GACTCGAGGC TTGCAAGACC CGGAAGTATC TGGCCAAGTT GCCCAATATC 
AGCGTGATTT TCATCTTCTT CAACGAGCAC TTTAACACCC TGTTGAGATC GATATACAGT 
GTGATAAATC GCACTCCACC GGAATTACTT AAGCAAATTG TGCTCGTGGA CGATGGCAGC 
GAGTGGGATG TTCTGAAGCA GCCTTTGGAC GACTACGTCC AGCAGCACTT TCCACATCTG 
GTGACTATAG TGCGCAATCC GGAGAGACAA GGTCTAATTG GAGCCAGAAT AGCTGGAGCC 
AAGGTGGCAG TGGGACAAGT GATGGTGTTC TTTGATTCTC ACATAGAAGT CAACTACAAT 
TGGCTGCCTC CCCTGATCGA ACCCATTGCC ATTAATCCCA AGATCTCCAC CTGCCCCATG 
GTGGATACTA TTTCGCATGA AGATTTCTCT TACTTTAGCG GAAATAAGGA CGGAGCACGA 
GGTGGATTCG ATTGGAAGAT GCTGTACAAA CAGCTTCCGG TTCTGCCCGA AGATGCGCTG 
GACAAGTCCA TGCCGTACCG GAGTCCCGTG ATGATGGGCG GTCTATTTGC CATCAACACG 
GACTTCTTTT GGGATCTGGG CGGATACGAC GACCAGTTGG ACATCTGGGG CGGTGAGCAG 
TACGAGCTGA GTTTCAAGAT CTGGATGTGT GGCGGTATGC TGCTGGATGT TCCCTGCTCG 
AGGGTGGCAC ACATATTCCG GGGACCCATG AAGCCAAGGG GCAATCCAAG GGGACACAAC 
TTTGTGGCCA AGAACCACAA GCGCGTGGCA GAGGTTTGGA TGGATGAGTA TAAGCAATAT 
GTGTACAAAC GCGATCCGAA GACCTATGAC AACCTGGATG CCGGAGATCT GACCCGCCAG 
CGTGGAGTTC GAGAGCGACT CAAGTGCAAG AGTTTCCACT GGTTCATGAC GGAGGTGGCG 
CCTGATTTCC TCGTCAAATT CCCGCCCGTG GAACCACCTA GCTACGCCGC CGGCATCATT 
CAGAACGTGG CCAACCCGGT CTATTGCCTG GACAACATGG GCAAGTCCAC CGAGGAGGCG 
GTGGGTATGT TCAGCTGTGC GGACAATAGG ACCCATCCGC AACCGAATCA GTTTTGGGAG 
CTCTCCATAT TCCGAGACCT CCGGATGAAG GGCTTCGATT CAGTGTGCCT GGATGTGCAC 
GAGGGACCGC CGAATGCCAC CGTTTGGATG TGGAGCTGCC ACAGTCAGGG TGGCAATCAG 
TTCTGGTACT ATGACAGGCA AACCCAAAGG TTGGTTCACG GAGAGAACAA CAAGCGCTGC 
CTGGAGGGAT TTGTGGAGAA CGGCATAGCC AAGGTGGTGG CCAATTCCTG CGAGAATGGC 
AACGATCGCC AGCGCTGGGA GTTCGGATTC GTGAACCACA CTATGCTGGA CACCTTCTAC 
GATGGATTAA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137779.1
CDS	69..2003
Translation	MAIKKRYVKRLLRKVVLLLVVIVTVSLVTTLVVERRMKNAAELTEQLDPNGDPITPVFRAANIHPTRKAPRPPFQDRNSVVDIPRSDKLQGFRLPEPKGERKDWHDYAAMEADRKRSGFGEHGVAVKIENPDEKQLEKEHYEMNGFNGLISDRISVNRSVPDLRLEACKTRKYLAKLPNISVIFIFFNEHFNTLLRSIYSVINRTPPELLKQIVLVDDGSEWDVLKQPLDDYVQQHFPHLVTIVRNPERQGLIGARIAGAKVAVGQVMVFFDSHIEVNYNWLPPLIEPIAINPKISTCPMVDTISHEDFSYFSGNKDGARGGFDWKMLYKQLPVLPEDALDKSMPYRSPVMMGGLFAINTDFFWDLGGYDDQLDIWGGEQYELSFKIWMCGGMLLDVPCSRVAHIFRGPMKPRGNPRGHNFVAKNHKRVAEVWMDEYKQYVYKRDPKTYDNLDAGDLTRQRGVRERLKCKSFHWFMTEVAPDFLVKFPPVEPPSYAAGIIQNVANPVYCLDNMGKSTEEAVGMFSCADNRTHPQPNQFWELSIFRDLRMKGFDSVCLDVHEGPPNATVWMWSCHSQGGNQFWYYDRQTQRLVHGENNKRCLEGFVENGIAKVVANSCENGNDRQRWEFGFVNHTMLDTFYDGLK

Target ORF information:

RefSeq Version	NM_001144307.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster polypeptide N-Acetylgalactosaminyltransferase 4, transcript variant B (Pgant4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144307.2

ORF Insert Sequence:

ATGGCCATAA AAAAGAGATA TGTAAAGCGC CTGTTGCGCA AGGTGGTCCT CCTCTTGGTG 
GTCATCGTCA CTGTGTCCTT GGTCACCACT TTGGTGGTCG AACGGCGAAT GAAAAATGCG 
GCGGAGCTGA CGGAACAACT CGATCCGAAT GGAGATCCCA TAACGCCCGT TTTTAGGGCT 
GCCAATATAC ATCCAACTCG GAAAGCGCCA CGACCACCAT TTCAGGATCG CAACTCCGTG 
GTAGATATTC CGAGAAGTGA CAAACTCCAG GGATTCCGAC TACCGGAACC GAAGGGAGAG 
CGCAAGGATT GGCACGACTA TGCGGCCATG GAGGCGGATA GAAAGCGATC CGGTTTCGGT 
GAGCACGGAG TAGCCGTAAA GATCGAAAAT CCCGATGAGA AGCAGTTGGA AAAGGAGCAC 
TACGAGATGA ACGGCTTTAA TGGCCTCATA TCGGATAGAA TCTCCGTCAA TCGATCTGTG 
CCAGACCTCA GACTCGAGGC TTGCAAGACC CGGAAGTATC TGGCCAAGTT GCCCAATATC 
AGCGTGATTT TCATCTTCTT CAACGAGCAC TTTAACACCC TGTTGAGATC GATATACAGT 
GTGATAAATC GCACTCCACC GGAATTACTT AAGCAAATTG TGCTCGTGGA CGATGGCAGC 
GAGTGGGATG TTCTGAAGCA GCCTTTGGAC GACTACGTCC AGCAGCACTT TCCACATCTG 
GTGACTATAG TGCGCAATCC GGAGAGACAA GGTCTAATTG GAGCCAGAAT AGCTGGAGCC 
AAGGTGGCAG TGGGACAAGT GATGGTGTTC TTTGATTCTC ACATAGAAGT CAACTACAAT 
TGGCTGCCTC CCCTGATCGA ACCCATTGCC ATTAATCCCA AGATCTCCAC CTGCCCCATG 
GTGGATACTA TTTCGCATGA AGATTTCTCT TACTTTAGCG GAAATAAGGA CGGAGCACGA 
GGTGGATTCG ATTGGAAGAT GCTGTACAAA CAGCTTCCGG TTCTGCCCGA AGATGCGCTG 
GACAAGTCCA TGCCGTACCG GAGTCCCGTG ATGATGGGCG GTCTATTTGC CATCAACACG 
GACTTCTTTT GGGATCTGGG CGGATACGAC GACCAGTTGG ACATCTGGGG CGGTGAGCAG 
TACGAGCTGA GTTTCAAGAT CTGGATGTGT GGCGGTATGC TGCTGGATGT TCCCTGCTCG 
AGGGTGGCAC ACATATTCCG GGGACCCATG AAGCCAAGGG GCAATCCAAG GGGACACAAC 
TTTGTGGCCA AGAACCACAA GCGCGTGGCA GAGGTTTGGA TGGATGAGTA TAAGCAATAT 
GTGTACAAAC GCGATCCGAA GACCTATGAC AACCTGGATG CCGGAGATCT GACCCGCCAG 
CGTGGAGTTC GAGAGCGACT CAAGTGCAAG AGTTTCCACT GGTTCATGAC GGAGGTGGCG 
CCTGATTTCC TCGTCAAATT CCCGCCCGTG GAACCACCTA GCTACGCCGC CGGCATCATT 
CAGAACGTGG CCAACCCGGT CTATTGCCTG GACAACATGG GCAAGTCCAC CGAGGAGGCG 
GTGGGTATGT TCAGCTGTGC GGACAATAGG ACCCATCCGC AACCGAATCA GTTTTGGGAG 
CTCTCCATAT TCCGAGACCT CCGGATGAAG GGCTTCGATT CAGTGTGCCT GGATGTGCAC 
GAGGGACCGC CGAATGCCAC CGTTTGGATG TGGAGCTGCC ACAGTCAGGG TGGCAATCAG 
TTCTGGTACT ATGACAGGCA AACCCAAAGG TTGGTTCACG GAGAGAACAA CAAGCGCTGC 
CTGGAGGGAT TTGTGGAGAA CGGCATAGCC AAGGTGGTGG CCAATTCCTG CGAGAATGGC 
AACGATCGCC AGCGCTGGGA GTTCGGATTC GTGAACCACA CTATGCTGGA CACCTTCTAC 
GATGGATTAA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Pgant4 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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