vas cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	vas
Entrez Gene ID	26067080
Full Name	vasa
Synonyms	BG:DS00929.14,CG3506,CG43081,CG46283,DmRH25,Dmel\CG46283,Dmel_CG33678,Dmel_CG3506,Dmel_CG43081,EP(2)0812,VAS,VASA,Vas,Vasa,cgt,fs(2)ltoRJ36
General protein information	Preferred Names vasa Names CG46283 gene product from transcript CG46283-RB CG46283-PA CG46283-PB CG46283-PC VASA Vas courgette female sterile(2)ltoRJ36 no-relish vas-PA vas-PB vas-PC
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 35C1-35C1

mRNA and Protein(s)

mRNA	Protein	Name
NM_165103.3	NP_723899.1	vasa, isoform A
NM_001273529.2	NP_001260458.1	vasa, isoform B
NM_001316393.1	NP_001303322.1	vasa, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study shows that, in vivo, the localization of Drosophila Vasa to the nuage and germ plasm depends on its interaction with LOTUS domain proteins. The binding and stimulation of Vasa DEAD-box helicases by LOTUS domains are widely conserved.
Title: The LOTUS domain is a conserved DEAD-box RNA helicase regulator essential for the recruitment of Vasa to the germ plasm and nuage.

The study suggests that both the localization and the translation of gurken mRNA in the vitellarium is independent from Vasa.
Title: Combining the auxin-inducible degradation system with CRISPR/Cas9-based genome editing for the conditional depletion of endogenous Drosophila melanogaster proteins.

Replacing Trp660 by Glu (W660E) abolishes the ability of Vas to support germ cell formation and embryonic patterning and greatly reduces Vas activity in piRNA biogenesis, as measured by transposon silencing, and in activating Grk translation; results indicate that the unique C-terminus of Vas is essential for its function in piRNA biogenesis and that the conserved Trp660 residue has an important functional role.
Title: C-terminal residues specific to Vasa among DEAD-box helicases are required for its functions in piRNA biogenesis and embryonic patterning.

The following vas gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the vas cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa14703	NM_001273529.2 Latest version!	Drosophila melanogaster vasa (vas), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OFa14703	NM_165103.3 Latest version!	Drosophila melanogaster vasa (vas), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OFa14703	NM_001316393.1 Latest version!	Drosophila melanogaster vasa (vas), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

vas ( NM_165103.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_723899.1 Drosophila melanogaster vasa (vas), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa14703
Clone ID Related Accession (Same CDS sequence)	NM_165103.3 , NM_001273529.2 , NM_001316393.1
Accession Version	NM_165103.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vasa, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_165103.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGACG ACTGGGATGA TGAGCCCATT GTTGATACTC GCGGCGCCCG CGGTGGAGAT 
TGGAGCGATG ATGAGGACAC GGCCAAGAGC TTCAGCGGCG AAGCTGAAGG CGATGGTGTT 
GGAGGGAGCG GTGGTGAAGG CGGCGGCTAC CAAGGAGGAA ATCGAGATGT GTTCGGAAGG 
ATCGGCGGAG GCAGAGGAGG AGGAGCTGGA GGTTATCGAG GAGGAAATCG AGATGGAGGG 
GGCTTCCACG GTGGACGTCG CGAGGGAGAA AGGGACTTCC GCGGTGGAGA AGGCGGCTTC 
CGCGGTGGAC AAGGCGGCTC CCGCGGTGGA CAAGGCGGCT CCCGCGGGGG ACAAGGCGGC 
TTCCGTGGTG GAGAAGGCGG CTTCCGCGGT CGGCTGTACG AAAACGAGGA TGGTGATGAA 
CGTCGTGGAA GACTAGATCG GGAGGAACGC GGCGGTGAAC GTCGTGGAAG ACTAGATCGG 
GAGGAACGCG GCGGTGAACG TGGTGAAAGA GGGGACGGTG GTTTTGCGAG GCGCCGTCGA 
AATGAAGATG ATATAAATAA TAATAATAAT ATCGTCGAAG ATGTCGAAAG GAAGCGAGAG 
TTTTATATTC CTCCCGAGCC GTCCAACGAT GCAATTGAGA TATTCAGCAG TGGCATAGCT 
TCCGGGATTC ATTTTTCGAA ATACAACAAC ATACCGGTTA AGGTGACCGG CAGCGATGTT 
CCACAACCTA TACAACATTT TACGAGCGCT GATTTGAGAG ATATTATTAT AGACAATGTA 
AACAAATCTG GATACAAAAT ACCGACACCA ATTCAAAAGT GTTCCATACC AGTAATATCC 
TCTGGTCGAG ACCTAATGGC GTGTGCCCAG ACGGGTTCTG GAAAAACGGC AGCTTTTTTG 
TTGCCCATTT TGAGCAAGTT GTTGGAGGAT CCCCATGAAC TGGAGCTTGG AAGACCCCAG 
GTAGTGATTG TATCCCCAAC AAGGGAGCTG GCAATTCAGA TCTTTAACGA GGCGAGGAAG 
TTTGCTTTCG AGTCATATCT AAAGATCGGT ATTGTTTACG GAGGCACCTC GTTCAGACAT 
CAAAACGAGT GCATTACCAG AGGCTGCCAT GTAGTGATCG CCACTCCGGG ACGACTTCTG 
GATTTCGTGG ATCGGACCTT TATCACGTTT GAAGACACTC GATTCGTTGT GCTGGATGAG 
GCCGATCGCA TGCTAGATAT GGGTTTCTCA GAAGACATGC GAAGAATCAT GACGCATGTA 
ACTATGCGTC CAGAACATCA GACATTGATG TTTTCCGCCA CGTTTCCAGA AGAAATCCAA 
AGAATGGCCG GCGAATTCCT TAAAAATTAC GTTTTCGTCG CCATTGGCAT TGTAGGCGGA 
GCTTGCTCTG ATGTGAAGCA GACCATATAC GAAGTTAATA AGTACGCCAA GCGATCCAAG 
CTAATAGAAA TCCTTTCCGA GCAAGCAGAT GGCACCATTG TGTTTGTGGA GACAAAGCGT 
GGCGCCGACT TTTTAGCTTC CTTCCTGTCA GAAAAGGAGT TTCCGACGAC CTCCATTCAT 
GGCGATCGTC TCCAGAGTCA ACGCGAGCAG GCCTTGCGTG ATTTCAAGAA CGGCTCTATG 
AAGGTTCTCA TAGCCACTTC AGTGGCTTCT CGTGGATTAG ACATTAAAAA CATCAAGCAT 
GTGATCAACT ATGACATGCC CAGCAAGATC GATGACTACG TACATCGCAT TGGACGTACA 
GGTCGTGTAG GCAATAATGG ACGAGCCACA AGCTTCTTTG ACCCTGAAAA GGATCGAGCT 
ATTGCTGCGG ACTTGGTAAA AATCTTGGAG GGATCTGGCC AGACTGTTCC GGACTTTCTA 
CGCACCTGTG GTGCCGGCGG TGATGGGGGT TACTCCAATC AAAATTTCGG TGGGGTGGAT 
GTGCGTGGCA GGGGAAATTA CGTCGGCGAT GCCACCAATG TCGAGGAAGA AGAGCAATGG 
GATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_723899.1
CDS	152..2137
Translation	MSDDWDDEPIVDTRGARGGDWSDDEDTAKSFSGEAEGDGVGGSGGEGGGYQGGNRDVFGRIGGGRGGGAGGYRGGNRDGGGFHGGRREGERDFRGGEGGFRGGQGGSRGGQGGSRGGQGGFRGGEGGFRGRLYENEDGDERRGRLDREERGGERRGRLDREERGGERGERGDGGFARRRRNEDDINNNNNIVEDVERKREFYIPPEPSNDAIEIFSSGIASGIHFSKYNNIPVKVTGSDVPQPIQHFTSADLRDIIIDNVNKSGYKIPTPIQKCSIPVISSGRDLMACAQTGSGKTAAFLLPILSKLLEDPHELELGRPQVVIVSPTRELAIQIFNEARKFAFESYLKIGIVYGGTSFRHQNECITRGCHVVIATPGRLLDFVDRTFITFEDTRFVVLDEADRMLDMGFSEDMRRIMTHVTMRPEHQTLMFSATFPEEIQRMAGEFLKNYVFVAIGIVGGACSDVKQTIYEVNKYAKRSKLIEILSEQADGTIVFVETKRGADFLASFLSEKEFPTTSIHGDRLQSQREQALRDFKNGSMKVLIATSVASRGLDIKNIKHVINYDMPSKIDDYVHRIGRTGRVGNNGRATSFFDPEKDRAIAADLVKILEGSGQTVPDFLRTCGAGGDGGYSNQNFGGVDVRGRGNYVGDATNVEEEEQWD

Target ORF information:

RefSeq Version	NM_165103.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vasa (vas), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165103.3

ORF Insert Sequence:

ATGTCTGACG ACTGGGATGA TGAGCCCATT GTTGATACTC GCGGCGCCCG CGGTGGAGAT 
TGGAGCGATG ATGAGGACAC GGCCAAGAGC TTCAGCGGCG AAGCTGAAGG CGATGGTGTT 
GGAGGGAGCG GTGGTGAAGG CGGCGGCTAC CAAGGAGGAA ATCGAGATGT GTTCGGAAGG 
ATCGGCGGAG GCAGAGGAGG AGGAGCTGGA GGTTATCGAG GAGGAAATCG AGATGGAGGG 
GGCTTCCACG GTGGACGTCG CGAGGGAGAA AGGGACTTCC GCGGTGGAGA AGGCGGCTTC 
CGCGGTGGAC AAGGCGGCTC CCGCGGTGGA CAAGGCGGCT CCCGCGGGGG ACAAGGCGGC 
TTCCGTGGTG GAGAAGGCGG CTTCCGCGGT CGGCTGTACG AAAACGAGGA TGGTGATGAA 
CGTCGTGGAA GACTAGATCG GGAGGAACGC GGCGGTGAAC GTCGTGGAAG ACTAGATCGG 
GAGGAACGCG GCGGTGAACG TGGTGAAAGA GGGGACGGTG GTTTTGCGAG GCGCCGTCGA 
AATGAAGATG ATATAAATAA TAATAATAAT ATCGTCGAAG ATGTCGAAAG GAAGCGAGAG 
TTTTATATTC CTCCCGAGCC GTCCAACGAT GCAATTGAGA TATTCAGCAG TGGCATAGCT 
TCCGGGATTC ATTTTTCGAA ATACAACAAC ATACCGGTTA AGGTGACCGG CAGCGATGTT 
CCACAACCTA TACAACATTT TACGAGCGCT GATTTGAGAG ATATTATTAT AGACAATGTA 
AACAAATCTG GATACAAAAT ACCGACACCA ATTCAAAAGT GTTCCATACC AGTAATATCC 
TCTGGTCGAG ACCTAATGGC GTGTGCCCAG ACGGGTTCTG GAAAAACGGC AGCTTTTTTG 
TTGCCCATTT TGAGCAAGTT GTTGGAGGAT CCCCATGAAC TGGAGCTTGG AAGACCCCAG 
GTAGTGATTG TATCCCCAAC AAGGGAGCTG GCAATTCAGA TCTTTAACGA GGCGAGGAAG 
TTTGCTTTCG AGTCATATCT AAAGATCGGT ATTGTTTACG GAGGCACCTC GTTCAGACAT 
CAAAACGAGT GCATTACCAG AGGCTGCCAT GTAGTGATCG CCACTCCGGG ACGACTTCTG 
GATTTCGTGG ATCGGACCTT TATCACGTTT GAAGACACTC GATTCGTTGT GCTGGATGAG 
GCCGATCGCA TGCTAGATAT GGGTTTCTCA GAAGACATGC GAAGAATCAT GACGCATGTA 
ACTATGCGTC CAGAACATCA GACATTGATG TTTTCCGCCA CGTTTCCAGA AGAAATCCAA 
AGAATGGCCG GCGAATTCCT TAAAAATTAC GTTTTCGTCG CCATTGGCAT TGTAGGCGGA 
GCTTGCTCTG ATGTGAAGCA GACCATATAC GAAGTTAATA AGTACGCCAA GCGATCCAAG 
CTAATAGAAA TCCTTTCCGA GCAAGCAGAT GGCACCATTG TGTTTGTGGA GACAAAGCGT 
GGCGCCGACT TTTTAGCTTC CTTCCTGTCA GAAAAGGAGT TTCCGACGAC CTCCATTCAT 
GGCGATCGTC TCCAGAGTCA ACGCGAGCAG GCCTTGCGTG ATTTCAAGAA CGGCTCTATG 
AAGGTTCTCA TAGCCACTTC AGTGGCTTCT CGTGGATTAG ACATTAAAAA CATCAAGCAT 
GTGATCAACT ATGACATGCC CAGCAAGATC GATGACTACG TACATCGCAT TGGACGTACA 
GGTCGTGTAG GCAATAATGG ACGAGCCACA AGCTTCTTTG ACCCTGAAAA GGATCGAGCT 
ATTGCTGCGG ACTTGGTAAA AATCTTGGAG GGATCTGGCC AGACTGTTCC GGACTTTCTA 
CGCACCTGTG GTGCCGGCGG TGATGGGGGT TACTCCAATC AAAATTTCGG TGGGGTGGAT 
GTGCGTGGCA GGGGAAATTA CGTCGGCGAT GCCACCAATG TCGAGGAAGA AGAGCAATGG 
GATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vas ( NM_001273529.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260458.1 Drosophila melanogaster vasa (vas), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa14703
Clone ID Related Accession (Same CDS sequence)	NM_165103.3 , NM_001273529.2 , NM_001316393.1
Accession Version	NM_001273529.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vasa, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Oct 19, 2015 this sequence version replaced NM_001273529.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGACG ACTGGGATGA TGAGCCCATT GTTGATACTC GCGGCGCCCG CGGTGGAGAT 
TGGAGCGATG ATGAGGACAC GGCCAAGAGC TTCAGCGGCG AAGCTGAAGG CGATGGTGTT 
GGAGGGAGCG GTGGTGAAGG CGGCGGCTAC CAAGGAGGAA ATCGAGATGT GTTCGGAAGG 
ATCGGCGGAG GCAGAGGAGG AGGAGCTGGA GGTTATCGAG GAGGAAATCG AGATGGAGGG 
GGCTTCCACG GTGGACGTCG CGAGGGAGAA AGGGACTTCC GCGGTGGAGA AGGCGGCTTC 
CGCGGTGGAC AAGGCGGCTC CCGCGGTGGA CAAGGCGGCT CCCGCGGGGG ACAAGGCGGC 
TTCCGTGGTG GAGAAGGCGG CTTCCGCGGT CGGCTGTACG AAAACGAGGA TGGTGATGAA 
CGTCGTGGAA GACTAGATCG GGAGGAACGC GGCGGTGAAC GTCGTGGAAG ACTAGATCGG 
GAGGAACGCG GCGGTGAACG TGGTGAAAGA GGGGACGGTG GTTTTGCGAG GCGCCGTCGA 
AATGAAGATG ATATAAATAA TAATAATAAT ATCGTCGAAG ATGTCGAAAG GAAGCGAGAG 
TTTTATATTC CTCCCGAGCC GTCCAACGAT GCAATTGAGA TATTCAGCAG TGGCATAGCT 
TCCGGGATTC ATTTTTCGAA ATACAACAAC ATACCGGTTA AGGTGACCGG CAGCGATGTT 
CCACAACCTA TACAACATTT TACGAGCGCT GATTTGAGAG ATATTATTAT AGACAATGTA 
AACAAATCTG GATACAAAAT ACCGACACCA ATTCAAAAGT GTTCCATACC AGTAATATCC 
TCTGGTCGAG ACCTAATGGC GTGTGCCCAG ACGGGTTCTG GAAAAACGGC AGCTTTTTTG 
TTGCCCATTT TGAGCAAGTT GTTGGAGGAT CCCCATGAAC TGGAGCTTGG AAGACCCCAG 
GTAGTGATTG TATCCCCAAC AAGGGAGCTG GCAATTCAGA TCTTTAACGA GGCGAGGAAG 
TTTGCTTTCG AGTCATATCT AAAGATCGGT ATTGTTTACG GAGGCACCTC GTTCAGACAT 
CAAAACGAGT GCATTACCAG AGGCTGCCAT GTAGTGATCG CCACTCCGGG ACGACTTCTG 
GATTTCGTGG ATCGGACCTT TATCACGTTT GAAGACACTC GATTCGTTGT GCTGGATGAG 
GCCGATCGCA TGCTAGATAT GGGTTTCTCA GAAGACATGC GAAGAATCAT GACGCATGTA 
ACTATGCGTC CAGAACATCA GACATTGATG TTTTCCGCCA CGTTTCCAGA AGAAATCCAA 
AGAATGGCCG GCGAATTCCT TAAAAATTAC GTTTTCGTCG CCATTGGCAT TGTAGGCGGA 
GCTTGCTCTG ATGTGAAGCA GACCATATAC GAAGTTAATA AGTACGCCAA GCGATCCAAG 
CTAATAGAAA TCCTTTCCGA GCAAGCAGAT GGCACCATTG TGTTTGTGGA GACAAAGCGT 
GGCGCCGACT TTTTAGCTTC CTTCCTGTCA GAAAAGGAGT TTCCGACGAC CTCCATTCAT 
GGCGATCGTC TCCAGAGTCA ACGCGAGCAG GCCTTGCGTG ATTTCAAGAA CGGCTCTATG 
AAGGTTCTCA TAGCCACTTC AGTGGCTTCT CGTGGATTAG ACATTAAAAA CATCAAGCAT 
GTGATCAACT ATGACATGCC CAGCAAGATC GATGACTACG TACATCGCAT TGGACGTACA 
GGTCGTGTAG GCAATAATGG ACGAGCCACA AGCTTCTTTG ACCCTGAAAA GGATCGAGCT 
ATTGCTGCGG ACTTGGTAAA AATCTTGGAG GGATCTGGCC AGACTGTTCC GGACTTTCTA 
CGCACCTGTG GTGCCGGCGG TGATGGGGGT TACTCCAATC AAAATTTCGG TGGGGTGGAT 
GTGCGTGGCA GGGGAAATTA CGTCGGCGAT GCCACCAATG TCGAGGAAGA AGAGCAATGG 
GATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260458.1
CDS	152..2137
Translation	MSDDWDDEPIVDTRGARGGDWSDDEDTAKSFSGEAEGDGVGGSGGEGGGYQGGNRDVFGRIGGGRGGGAGGYRGGNRDGGGFHGGRREGERDFRGGEGGFRGGQGGSRGGQGGSRGGQGGFRGGEGGFRGRLYENEDGDERRGRLDREERGGERRGRLDREERGGERGERGDGGFARRRRNEDDINNNNNIVEDVERKREFYIPPEPSNDAIEIFSSGIASGIHFSKYNNIPVKVTGSDVPQPIQHFTSADLRDIIIDNVNKSGYKIPTPIQKCSIPVISSGRDLMACAQTGSGKTAAFLLPILSKLLEDPHELELGRPQVVIVSPTRELAIQIFNEARKFAFESYLKIGIVYGGTSFRHQNECITRGCHVVIATPGRLLDFVDRTFITFEDTRFVVLDEADRMLDMGFSEDMRRIMTHVTMRPEHQTLMFSATFPEEIQRMAGEFLKNYVFVAIGIVGGACSDVKQTIYEVNKYAKRSKLIEILSEQADGTIVFVETKRGADFLASFLSEKEFPTTSIHGDRLQSQREQALRDFKNGSMKVLIATSVASRGLDIKNIKHVINYDMPSKIDDYVHRIGRTGRVGNNGRATSFFDPEKDRAIAADLVKILEGSGQTVPDFLRTCGAGGDGGYSNQNFGGVDVRGRGNYVGDATNVEEEEQWD

Target ORF information:

RefSeq Version	NM_001273529.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vasa (vas), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273529.2

ORF Insert Sequence:

ATGTCTGACG ACTGGGATGA TGAGCCCATT GTTGATACTC GCGGCGCCCG CGGTGGAGAT 
TGGAGCGATG ATGAGGACAC GGCCAAGAGC TTCAGCGGCG AAGCTGAAGG CGATGGTGTT 
GGAGGGAGCG GTGGTGAAGG CGGCGGCTAC CAAGGAGGAA ATCGAGATGT GTTCGGAAGG 
ATCGGCGGAG GCAGAGGAGG AGGAGCTGGA GGTTATCGAG GAGGAAATCG AGATGGAGGG 
GGCTTCCACG GTGGACGTCG CGAGGGAGAA AGGGACTTCC GCGGTGGAGA AGGCGGCTTC 
CGCGGTGGAC AAGGCGGCTC CCGCGGTGGA CAAGGCGGCT CCCGCGGGGG ACAAGGCGGC 
TTCCGTGGTG GAGAAGGCGG CTTCCGCGGT CGGCTGTACG AAAACGAGGA TGGTGATGAA 
CGTCGTGGAA GACTAGATCG GGAGGAACGC GGCGGTGAAC GTCGTGGAAG ACTAGATCGG 
GAGGAACGCG GCGGTGAACG TGGTGAAAGA GGGGACGGTG GTTTTGCGAG GCGCCGTCGA 
AATGAAGATG ATATAAATAA TAATAATAAT ATCGTCGAAG ATGTCGAAAG GAAGCGAGAG 
TTTTATATTC CTCCCGAGCC GTCCAACGAT GCAATTGAGA TATTCAGCAG TGGCATAGCT 
TCCGGGATTC ATTTTTCGAA ATACAACAAC ATACCGGTTA AGGTGACCGG CAGCGATGTT 
CCACAACCTA TACAACATTT TACGAGCGCT GATTTGAGAG ATATTATTAT AGACAATGTA 
AACAAATCTG GATACAAAAT ACCGACACCA ATTCAAAAGT GTTCCATACC AGTAATATCC 
TCTGGTCGAG ACCTAATGGC GTGTGCCCAG ACGGGTTCTG GAAAAACGGC AGCTTTTTTG 
TTGCCCATTT TGAGCAAGTT GTTGGAGGAT CCCCATGAAC TGGAGCTTGG AAGACCCCAG 
GTAGTGATTG TATCCCCAAC AAGGGAGCTG GCAATTCAGA TCTTTAACGA GGCGAGGAAG 
TTTGCTTTCG AGTCATATCT AAAGATCGGT ATTGTTTACG GAGGCACCTC GTTCAGACAT 
CAAAACGAGT GCATTACCAG AGGCTGCCAT GTAGTGATCG CCACTCCGGG ACGACTTCTG 
GATTTCGTGG ATCGGACCTT TATCACGTTT GAAGACACTC GATTCGTTGT GCTGGATGAG 
GCCGATCGCA TGCTAGATAT GGGTTTCTCA GAAGACATGC GAAGAATCAT GACGCATGTA 
ACTATGCGTC CAGAACATCA GACATTGATG TTTTCCGCCA CGTTTCCAGA AGAAATCCAA 
AGAATGGCCG GCGAATTCCT TAAAAATTAC GTTTTCGTCG CCATTGGCAT TGTAGGCGGA 
GCTTGCTCTG ATGTGAAGCA GACCATATAC GAAGTTAATA AGTACGCCAA GCGATCCAAG 
CTAATAGAAA TCCTTTCCGA GCAAGCAGAT GGCACCATTG TGTTTGTGGA GACAAAGCGT 
GGCGCCGACT TTTTAGCTTC CTTCCTGTCA GAAAAGGAGT TTCCGACGAC CTCCATTCAT 
GGCGATCGTC TCCAGAGTCA ACGCGAGCAG GCCTTGCGTG ATTTCAAGAA CGGCTCTATG 
AAGGTTCTCA TAGCCACTTC AGTGGCTTCT CGTGGATTAG ACATTAAAAA CATCAAGCAT 
GTGATCAACT ATGACATGCC CAGCAAGATC GATGACTACG TACATCGCAT TGGACGTACA 
GGTCGTGTAG GCAATAATGG ACGAGCCACA AGCTTCTTTG ACCCTGAAAA GGATCGAGCT 
ATTGCTGCGG ACTTGGTAAA AATCTTGGAG GGATCTGGCC AGACTGTTCC GGACTTTCTA 
CGCACCTGTG GTGCCGGCGG TGATGGGGGT TACTCCAATC AAAATTTCGG TGGGGTGGAT 
GTGCGTGGCA GGGGAAATTA CGTCGGCGAT GCCACCAATG TCGAGGAAGA AGAGCAATGG 
GATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vas ( NM_001316393.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001303322.1 Drosophila melanogaster vasa (vas), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa14703
Clone ID Related Accession (Same CDS sequence)	NM_165103.3 , NM_001273529.2 , NM_001316393.1
Accession Version	NM_001316393.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vasa, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCTGACG ACTGGGATGA TGAGCCCATT GTTGATACTC GCGGCGCCCG CGGTGGAGAT 
TGGAGCGATG ATGAGGACAC GGCCAAGAGC TTCAGCGGCG AAGCTGAAGG CGATGGTGTT 
GGAGGGAGCG GTGGTGAAGG CGGCGGCTAC CAAGGAGGAA ATCGAGATGT GTTCGGAAGG 
ATCGGCGGAG GCAGAGGAGG AGGAGCTGGA GGTTATCGAG GAGGAAATCG AGATGGAGGG 
GGCTTCCACG GTGGACGTCG CGAGGGAGAA AGGGACTTCC GCGGTGGAGA AGGCGGCTTC 
CGCGGTGGAC AAGGCGGCTC CCGCGGTGGA CAAGGCGGCT CCCGCGGGGG ACAAGGCGGC 
TTCCGTGGTG GAGAAGGCGG CTTCCGCGGT CGGCTGTACG AAAACGAGGA TGGTGATGAA 
CGTCGTGGAA GACTAGATCG GGAGGAACGC GGCGGTGAAC GTCGTGGAAG ACTAGATCGG 
GAGGAACGCG GCGGTGAACG TGGTGAAAGA GGGGACGGTG GTTTTGCGAG GCGCCGTCGA 
AATGAAGATG ATATAAATAA TAATAATAAT ATCGTCGAAG ATGTCGAAAG GAAGCGAGAG 
TTTTATATTC CTCCCGAGCC GTCCAACGAT GCAATTGAGA TATTCAGCAG TGGCATAGCT 
TCCGGGATTC ATTTTTCGAA ATACAACAAC ATACCGGTTA AGGTGACCGG CAGCGATGTT 
CCACAACCTA TACAACATTT TACGAGCGCT GATTTGAGAG ATATTATTAT AGACAATGTA 
AACAAATCTG GATACAAAAT ACCGACACCA ATTCAAAAGT GTTCCATACC AGTAATATCC 
TCTGGTCGAG ACCTAATGGC GTGTGCCCAG ACGGGTTCTG GAAAAACGGC AGCTTTTTTG 
TTGCCCATTT TGAGCAAGTT GTTGGAGGAT CCCCATGAAC TGGAGCTTGG AAGACCCCAG 
GTAGTGATTG TATCCCCAAC AAGGGAGCTG GCAATTCAGA TCTTTAACGA GGCGAGGAAG 
TTTGCTTTCG AGTCATATCT AAAGATCGGT ATTGTTTACG GAGGCACCTC GTTCAGACAT 
CAAAACGAGT GCATTACCAG AGGCTGCCAT GTAGTGATCG CCACTCCGGG ACGACTTCTG 
GATTTCGTGG ATCGGACCTT TATCACGTTT GAAGACACTC GATTCGTTGT GCTGGATGAG 
GCCGATCGCA TGCTAGATAT GGGTTTCTCA GAAGACATGC GAAGAATCAT GACGCATGTA 
ACTATGCGTC CAGAACATCA GACATTGATG TTTTCCGCCA CGTTTCCAGA AGAAATCCAA 
AGAATGGCCG GCGAATTCCT TAAAAATTAC GTTTTCGTCG CCATTGGCAT TGTAGGCGGA 
GCTTGCTCTG ATGTGAAGCA GACCATATAC GAAGTTAATA AGTACGCCAA GCGATCCAAG 
CTAATAGAAA TCCTTTCCGA GCAAGCAGAT GGCACCATTG TGTTTGTGGA GACAAAGCGT 
GGCGCCGACT TTTTAGCTTC CTTCCTGTCA GAAAAGGAGT TTCCGACGAC CTCCATTCAT 
GGCGATCGTC TCCAGAGTCA ACGCGAGCAG GCCTTGCGTG ATTTCAAGAA CGGCTCTATG 
AAGGTTCTCA TAGCCACTTC AGTGGCTTCT CGTGGATTAG ACATTAAAAA CATCAAGCAT 
GTGATCAACT ATGACATGCC CAGCAAGATC GATGACTACG TACATCGCAT TGGACGTACA 
GGTCGTGTAG GCAATAATGG ACGAGCCACA AGCTTCTTTG ACCCTGAAAA GGATCGAGCT 
ATTGCTGCGG ACTTGGTAAA AATCTTGGAG GGATCTGGCC AGACTGTTCC GGACTTTCTA 
CGCACCTGTG GTGCCGGCGG TGATGGGGGT TACTCCAATC AAAATTTCGG TGGGGTGGAT 
GTGCGTGGCA GGGGAAATTA CGTCGGCGAT GCCACCAATG TCGAGGAAGA AGAGCAATGG 
GATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303322.1
CDS	106..2091
Translation	MSDDWDDEPIVDTRGARGGDWSDDEDTAKSFSGEAEGDGVGGSGGEGGGYQGGNRDVFGRIGGGRGGGAGGYRGGNRDGGGFHGGRREGERDFRGGEGGFRGGQGGSRGGQGGSRGGQGGFRGGEGGFRGRLYENEDGDERRGRLDREERGGERRGRLDREERGGERGERGDGGFARRRRNEDDINNNNNIVEDVERKREFYIPPEPSNDAIEIFSSGIASGIHFSKYNNIPVKVTGSDVPQPIQHFTSADLRDIIIDNVNKSGYKIPTPIQKCSIPVISSGRDLMACAQTGSGKTAAFLLPILSKLLEDPHELELGRPQVVIVSPTRELAIQIFNEARKFAFESYLKIGIVYGGTSFRHQNECITRGCHVVIATPGRLLDFVDRTFITFEDTRFVVLDEADRMLDMGFSEDMRRIMTHVTMRPEHQTLMFSATFPEEIQRMAGEFLKNYVFVAIGIVGGACSDVKQTIYEVNKYAKRSKLIEILSEQADGTIVFVETKRGADFLASFLSEKEFPTTSIHGDRLQSQREQALRDFKNGSMKVLIATSVASRGLDIKNIKHVINYDMPSKIDDYVHRIGRTGRVGNNGRATSFFDPEKDRAIAADLVKILEGSGQTVPDFLRTCGAGGDGGYSNQNFGGVDVRGRGNYVGDATNVEEEEQWD

Target ORF information:

RefSeq Version	NM_001316393.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vasa (vas), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316393.1

ORF Insert Sequence:

ATGTCTGACG ACTGGGATGA TGAGCCCATT GTTGATACTC GCGGCGCCCG CGGTGGAGAT 
TGGAGCGATG ATGAGGACAC GGCCAAGAGC TTCAGCGGCG AAGCTGAAGG CGATGGTGTT 
GGAGGGAGCG GTGGTGAAGG CGGCGGCTAC CAAGGAGGAA ATCGAGATGT GTTCGGAAGG 
ATCGGCGGAG GCAGAGGAGG AGGAGCTGGA GGTTATCGAG GAGGAAATCG AGATGGAGGG 
GGCTTCCACG GTGGACGTCG CGAGGGAGAA AGGGACTTCC GCGGTGGAGA AGGCGGCTTC 
CGCGGTGGAC AAGGCGGCTC CCGCGGTGGA CAAGGCGGCT CCCGCGGGGG ACAAGGCGGC 
TTCCGTGGTG GAGAAGGCGG CTTCCGCGGT CGGCTGTACG AAAACGAGGA TGGTGATGAA 
CGTCGTGGAA GACTAGATCG GGAGGAACGC GGCGGTGAAC GTCGTGGAAG ACTAGATCGG 
GAGGAACGCG GCGGTGAACG TGGTGAAAGA GGGGACGGTG GTTTTGCGAG GCGCCGTCGA 
AATGAAGATG ATATAAATAA TAATAATAAT ATCGTCGAAG ATGTCGAAAG GAAGCGAGAG 
TTTTATATTC CTCCCGAGCC GTCCAACGAT GCAATTGAGA TATTCAGCAG TGGCATAGCT 
TCCGGGATTC ATTTTTCGAA ATACAACAAC ATACCGGTTA AGGTGACCGG CAGCGATGTT 
CCACAACCTA TACAACATTT TACGAGCGCT GATTTGAGAG ATATTATTAT AGACAATGTA 
AACAAATCTG GATACAAAAT ACCGACACCA ATTCAAAAGT GTTCCATACC AGTAATATCC 
TCTGGTCGAG ACCTAATGGC GTGTGCCCAG ACGGGTTCTG GAAAAACGGC AGCTTTTTTG 
TTGCCCATTT TGAGCAAGTT GTTGGAGGAT CCCCATGAAC TGGAGCTTGG AAGACCCCAG 
GTAGTGATTG TATCCCCAAC AAGGGAGCTG GCAATTCAGA TCTTTAACGA GGCGAGGAAG 
TTTGCTTTCG AGTCATATCT AAAGATCGGT ATTGTTTACG GAGGCACCTC GTTCAGACAT 
CAAAACGAGT GCATTACCAG AGGCTGCCAT GTAGTGATCG CCACTCCGGG ACGACTTCTG 
GATTTCGTGG ATCGGACCTT TATCACGTTT GAAGACACTC GATTCGTTGT GCTGGATGAG 
GCCGATCGCA TGCTAGATAT GGGTTTCTCA GAAGACATGC GAAGAATCAT GACGCATGTA 
ACTATGCGTC CAGAACATCA GACATTGATG TTTTCCGCCA CGTTTCCAGA AGAAATCCAA 
AGAATGGCCG GCGAATTCCT TAAAAATTAC GTTTTCGTCG CCATTGGCAT TGTAGGCGGA 
GCTTGCTCTG ATGTGAAGCA GACCATATAC GAAGTTAATA AGTACGCCAA GCGATCCAAG 
CTAATAGAAA TCCTTTCCGA GCAAGCAGAT GGCACCATTG TGTTTGTGGA GACAAAGCGT 
GGCGCCGACT TTTTAGCTTC CTTCCTGTCA GAAAAGGAGT TTCCGACGAC CTCCATTCAT 
GGCGATCGTC TCCAGAGTCA ACGCGAGCAG GCCTTGCGTG ATTTCAAGAA CGGCTCTATG 
AAGGTTCTCA TAGCCACTTC AGTGGCTTCT CGTGGATTAG ACATTAAAAA CATCAAGCAT 
GTGATCAACT ATGACATGCC CAGCAAGATC GATGACTACG TACATCGCAT TGGACGTACA 
GGTCGTGTAG GCAATAATGG ACGAGCCACA AGCTTCTTTG ACCCTGAAAA GGATCGAGCT 
ATTGCTGCGG ACTTGGTAAA AATCTTGGAG GGATCTGGCC AGACTGTTCC GGACTTTCTA 
CGCACCTGTG GTGCCGGCGG TGATGGGGGT TACTCCAATC AAAATTTCGG TGGGGTGGAT 
GTGCGTGGCA GGGGAAATTA CGTCGGCGAT GCCACCAATG TCGAGGAAGA AGAGCAATGG 
GATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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vas cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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