Vha100-3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vha100-3
Entrez Gene ID	246543
Full Name	Vacuolar H[+] ATPase 100kD subunit 3
Synonyms	BcDNA:AT03238,CG30329,Dmel\CG30329,vha100-3
General protein information	Preferred Names Vacuolar H[+] ATPase 100kD subunit 3 Names CG30329 gene product from transcript CG30329-RA CG30329-PA Vha100-3-PA vacuolar H[+] ATPase 100kD subunit 3 vacuolar H[+] ATPase subunit 100-3
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 55F11-55F11

mRNA and Protein(s)

mRNA	Protein	Name
NM_166313.2	NP_725837.1	vacuolar H[+] ATPase 100kD subunit 3

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show a calmodulin-binding site in the large cytosolic N-terminal region of the Drosophila protein V100, that forms a tight complex with calmodulin in a Ca(2+)-dependent manner.
Title: V-ATPase V0 sector subunit a1 in neurons is a target of calmodulin.

The following Vha100-3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vha100-3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa11661	NM_166313.2 Latest version!	Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 3 (Vha100-3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vha100-3 ( NM_166313.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725837.1 Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 3 (Vha100-3), mRNA.

Price & Availability↑

CloneID	OFa11661
Clone ID Related Accession (Same CDS sequence)	NM_166313.2
Accession Version	NM_166313.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2715bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar H[+] ATPase 100kD subunit 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_166313.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGAGTAT TTAAGAAACG GCAGACGAAA GTCAAGTCCT TTTTCCGCAG CGAGGACATG 
GATTTGTGCC AACTGCTGCT GCACACGGAA AACGCCTTCG ATTGCCTCAT AGAGGTGGGA 
CACCATGGAG CAGTGCAGTT CAACAATGTC TACGATGAGG ACCGATTGCT GAACAACCTC 
TACTCCAAAA AGGTGACGCA GTGCTATGAG CTACTGCGCA TCGTGGACAG TCTGCACACT 
TACATCGTCC AATTGCATGT GAACGAAATA TTCTATCCGG ATGTCGATCG AGAGAATCGG 
CTTAAGGAGA AGGATCTGGC CAAGTATAGT GATAGTTTGA AACGCATCCA TGTGGAGGCC 
AGTGCCGTGA CGGAGCACTA CTACCGACTG GACAGTCGTC GAAATCGGAT GATGGAGCAC 
AGCTTCGCTT TGAACAAGGC CAACAAGTAT ATGGTTTCCG ACATGGGCAG TGAACTGCTC 
TATTCGGAGA GCACAGTGAT TGGTTTGGTT CAGGATGCCA CGACCACCTC TGGAGCTTAT 
CCGGCTCATC TGAACTATAT GATCGGCTGC ATCCGAGCTG ATAAATTCTA TAGCTTTGAG 
CTGCTGCTCT ACCGCTTGTG CTCCTTCAAC CTGATCATTC GATTTTCCGA AATGCCTAGT 
CCGGTCTACG AATATCATTA TGGGTATAAG CCGGAAAGGG TGCGCAAGTT TGCAATTCTC 
ATGATGGCCA GTTCAACGAT GATCTGGCCC AAGGTGCTTA AGATATGTGC TCACTATCAT 
GTTAACATCT ACGACTGCCC TAGTTCTGCC AGCCAGCGGG AGGATAAGGT TAAGGAGCTG 
AGCCAGGAGA TAGTGAACGT GGAGAAGGTC CTCAAGGAGG CCGAGCTGAT GCGCCGCCAG 
ATCCTGGAGG TTGCTGGTCG GGATCTATTC ATTATACGTG TGAATCTGCG CAAAGCGCTG 
AAGGTGTACG ATCTGATGAA TCGCCTGAGA TTGGTGGGTG GAGTAGAGGT ACCTCGTTAT 
CTGCTGGCCG AGGTGTATAT ACCATCGTCG GATGTTCCCG AAGTGGAGGT AATCCTACGA 
AATGCGTCCC GAATAAGTGG TGGTGCGGAT AATATTGATT CATCCGATGA AGACGAGATG 
AATGATATGA AAACAATGCC CAATACTACT CCCTATCCCA TTGAAGCCGA CTTCCAGCCG 
CTCGAGGATA TGAGTGCTGG AGCCATATTG CTAAAGAAAA ACAGACTGGT CAATCATATG 
CCACCGACGT ACTTTCGGCT AAACAAGTTC ACCAGGGGAT TCCAGAACCT AATAGACGCC 
TATGGTATGG CCGATTACAA GGAGTTGAAT CCCGCTCCGT ACACCATCAT CACATTCCCC 
TTCCTGTTTG CCGTTATGTT TGGCGATCTG GGTCATGGAA TTCTGCTAAT TTTGTTCTCC 
TCGCTCATGA TTTGGAAGCA CAGGGAGATC GAAAAGTATC AGATTAACGC GACCTCGGAG 
AACGAGATCC TGAATATCCT ATATGCGGGA CGCTACATAA TCTTACTGAT GGGTGTATTT 
TCCGTCTACA TGGGCCTAGT CTACAACATA GTCATGGCCA AGGGCTTCAA TCTTTTTGGT 
TCGAGCTGGA GTTGTCGATA CAATGAGACC ACTGTGTATG ATCCCGCTTT TCATGTGACC 
CTGGACTCTT CGCATCCTCA CTTCTACTCG GGCCATCCGT ATCCATTGGG AATGGATCCA 
GTGTGGGCCG TGTGCGGACA GGACTCGATA ACCACGACCA ATTCGTTGAA GATGAAGATG 
GCCATTGTCC TGGGCATATC ACAAATGATG TTTGGACTGG GCCTGGCTGC CGCTAATTGT 
GTGCTGATGA ATCGGAAAGC AGATCTCATT CTGGTGGTCA TTCCGCAGAT GATCTTCATG 
CTGTGCCTCT TCGGCTACCT CGTCTTTCTG ATCTTTTACA AGTGGATGTC GTACGGAGGC 
CATAAGCCGG CGCCCTACAA TGCTGCGTGT GCTCCCTCCG TTCTGATCAC CTTCATTAAC 
ATGATGCTGA TGAAAAAGGA AGACCCGGTG GAAAACTGCC TGGATTATAT GTATCCTAAT 
GAGCGGATGA TAGAGTTCGC CCTGGTAGGA ATAGCCTTTT GTACGATACC CATTTTATTG 
GCCGGCAAGC CGATATATCT CATGCGTCGT CGCAGAAAAA TGCAACAGGA ACGGGAACGG 
GATTTTAAGA GGATGCGACG TCAGACGATC GCGGAAATGC GATCCACAAT GCGGTACACG 
GATGATGATA ACAGTGAGAC CAGCAGGCAG AAAAGCGTTG ACAACGAGGA GGAGCACGAG 
ATGTCGGAGA TTTGGATTCA CTCTGGAATT CACACTATCG AAACGGTATT GGGCTCAGTA 
TCGCATACAG CCTCCTATCT TCGATTGTGG GCCCTCTCGT TGGCACACGA TCAGCTGTCC 
GATGTCCTGT GGCACATGGT TCTGACCAAG GGATTCGCCA ACACTCTGCC ATTGTACTAT 
GGTGTGCCCG TGCTCATGGC CACTTTCTTC GCCTGGGCCA TTCTCACAGT GGCCATACTG 
GTGATGATGG AGGGATTGAG CGCCTTCCTG CACACCCTGC GGCTCCACTG GGTGGAGTTC 
CAGTCGAAGT TCTTCGGCGG AGCGGGCGAG TCGTTCAAGG CATTCAATTT CCCGACGTCA 
AACCAGAGGA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725837.1
CDS	182..2896
Translation	MRVFKKRQTKVKSFFRSEDMDLCQLLLHTENAFDCLIEVGHHGAVQFNNVYDEDRLLNNLYSKKVTQCYELLRIVDSLHTYIVQLHVNEIFYPDVDRENRLKEKDLAKYSDSLKRIHVEASAVTEHYYRLDSRRNRMMEHSFALNKANKYMVSDMGSELLYSESTVIGLVQDATTTSGAYPAHLNYMIGCIRADKFYSFELLLYRLCSFNLIIRFSEMPSPVYEYHYGYKPERVRKFAILMMASSTMIWPKVLKICAHYHVNIYDCPSSASQREDKVKELSQEIVNVEKVLKEAELMRRQILEVAGRDLFIIRVNLRKALKVYDLMNRLRLVGGVEVPRYLLAEVYIPSSDVPEVEVILRNASRISGGADNIDSSDEDEMNDMKTMPNTTPYPIEADFQPLEDMSAGAILLKKNRLVNHMPPTYFRLNKFTRGFQNLIDAYGMADYKELNPAPYTIITFPFLFAVMFGDLGHGILLILFSSLMIWKHREIEKYQINATSENEILNILYAGRYIILLMGVFSVYMGLVYNIVMAKGFNLFGSSWSCRYNETTVYDPAFHVTLDSSHPHFYSGHPYPLGMDPVWAVCGQDSITTTNSLKMKMAIVLGISQMMFGLGLAAANCVLMNRKADLILVVIPQMIFMLCLFGYLVFLIFYKWMSYGGHKPAPYNAACAPSVLITFINMMLMKKEDPVENCLDYMYPNERMIEFALVGIAFCTIPILLAGKPIYLMRRRRKMQQERERDFKRMRRQTIAEMRSTMRYTDDDNSETSRQKSVDNEEEHEMSEIWIHSGIHTIETVLGSVSHTASYLRLWALSLAHDQLSDVLWHMVLTKGFANTLPLYYGVPVLMATFFAWAILTVAILVMMEGLSAFLHTLRLHWVEFQSKFFGGAGESFKAFNFPTSNQRS

Target ORF information:

RefSeq Version	NM_166313.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 3 (Vha100-3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166313.2

ORF Insert Sequence:

ATGAGAGTAT TTAAGAAACG GCAGACGAAA GTCAAGTCCT TTTTCCGCAG CGAGGACATG 
GATTTGTGCC AACTGCTGCT GCACACGGAA AACGCCTTCG ATTGCCTCAT AGAGGTGGGA 
CACCATGGAG CAGTGCAGTT CAACAATGTC TACGATGAGG ACCGATTGCT GAACAACCTC 
TACTCCAAAA AGGTGACGCA GTGCTATGAG CTACTGCGCA TCGTGGACAG TCTGCACACT 
TACATCGTCC AATTGCATGT GAACGAAATA TTCTATCCGG ATGTCGATCG AGAGAATCGG 
CTTAAGGAGA AGGATCTGGC CAAGTATAGT GATAGTTTGA AACGCATCCA TGTGGAGGCC 
AGTGCCGTGA CGGAGCACTA CTACCGACTG GACAGTCGTC GAAATCGGAT GATGGAGCAC 
AGCTTCGCTT TGAACAAGGC CAACAAGTAT ATGGTTTCCG ACATGGGCAG TGAACTGCTC 
TATTCGGAGA GCACAGTGAT TGGTTTGGTT CAGGATGCCA CGACCACCTC TGGAGCTTAT 
CCGGCTCATC TGAACTATAT GATCGGCTGC ATCCGAGCTG ATAAATTCTA TAGCTTTGAG 
CTGCTGCTCT ACCGCTTGTG CTCCTTCAAC CTGATCATTC GATTTTCCGA AATGCCTAGT 
CCGGTCTACG AATATCATTA TGGGTATAAG CCGGAAAGGG TGCGCAAGTT TGCAATTCTC 
ATGATGGCCA GTTCAACGAT GATCTGGCCC AAGGTGCTTA AGATATGTGC TCACTATCAT 
GTTAACATCT ACGACTGCCC TAGTTCTGCC AGCCAGCGGG AGGATAAGGT TAAGGAGCTG 
AGCCAGGAGA TAGTGAACGT GGAGAAGGTC CTCAAGGAGG CCGAGCTGAT GCGCCGCCAG 
ATCCTGGAGG TTGCTGGTCG GGATCTATTC ATTATACGTG TGAATCTGCG CAAAGCGCTG 
AAGGTGTACG ATCTGATGAA TCGCCTGAGA TTGGTGGGTG GAGTAGAGGT ACCTCGTTAT 
CTGCTGGCCG AGGTGTATAT ACCATCGTCG GATGTTCCCG AAGTGGAGGT AATCCTACGA 
AATGCGTCCC GAATAAGTGG TGGTGCGGAT AATATTGATT CATCCGATGA AGACGAGATG 
AATGATATGA AAACAATGCC CAATACTACT CCCTATCCCA TTGAAGCCGA CTTCCAGCCG 
CTCGAGGATA TGAGTGCTGG AGCCATATTG CTAAAGAAAA ACAGACTGGT CAATCATATG 
CCACCGACGT ACTTTCGGCT AAACAAGTTC ACCAGGGGAT TCCAGAACCT AATAGACGCC 
TATGGTATGG CCGATTACAA GGAGTTGAAT CCCGCTCCGT ACACCATCAT CACATTCCCC 
TTCCTGTTTG CCGTTATGTT TGGCGATCTG GGTCATGGAA TTCTGCTAAT TTTGTTCTCC 
TCGCTCATGA TTTGGAAGCA CAGGGAGATC GAAAAGTATC AGATTAACGC GACCTCGGAG 
AACGAGATCC TGAATATCCT ATATGCGGGA CGCTACATAA TCTTACTGAT GGGTGTATTT 
TCCGTCTACA TGGGCCTAGT CTACAACATA GTCATGGCCA AGGGCTTCAA TCTTTTTGGT 
TCGAGCTGGA GTTGTCGATA CAATGAGACC ACTGTGTATG ATCCCGCTTT TCATGTGACC 
CTGGACTCTT CGCATCCTCA CTTCTACTCG GGCCATCCGT ATCCATTGGG AATGGATCCA 
GTGTGGGCCG TGTGCGGACA GGACTCGATA ACCACGACCA ATTCGTTGAA GATGAAGATG 
GCCATTGTCC TGGGCATATC ACAAATGATG TTTGGACTGG GCCTGGCTGC CGCTAATTGT 
GTGCTGATGA ATCGGAAAGC AGATCTCATT CTGGTGGTCA TTCCGCAGAT GATCTTCATG 
CTGTGCCTCT TCGGCTACCT CGTCTTTCTG ATCTTTTACA AGTGGATGTC GTACGGAGGC 
CATAAGCCGG CGCCCTACAA TGCTGCGTGT GCTCCCTCCG TTCTGATCAC CTTCATTAAC 
ATGATGCTGA TGAAAAAGGA AGACCCGGTG GAAAACTGCC TGGATTATAT GTATCCTAAT 
GAGCGGATGA TAGAGTTCGC CCTGGTAGGA ATAGCCTTTT GTACGATACC CATTTTATTG 
GCCGGCAAGC CGATATATCT CATGCGTCGT CGCAGAAAAA TGCAACAGGA ACGGGAACGG 
GATTTTAAGA GGATGCGACG TCAGACGATC GCGGAAATGC GATCCACAAT GCGGTACACG 
GATGATGATA ACAGTGAGAC CAGCAGGCAG AAAAGCGTTG ACAACGAGGA GGAGCACGAG 
ATGTCGGAGA TTTGGATTCA CTCTGGAATT CACACTATCG AAACGGTATT GGGCTCAGTA 
TCGCATACAG CCTCCTATCT TCGATTGTGG GCCCTCTCGT TGGCACACGA TCAGCTGTCC 
GATGTCCTGT GGCACATGGT TCTGACCAAG GGATTCGCCA ACACTCTGCC ATTGTACTAT 
GGTGTGCCCG TGCTCATGGC CACTTTCTTC GCCTGGGCCA TTCTCACAGT GGCCATACTG 
GTGATGATGG AGGGATTGAG CGCCTTCCTG CACACCCTGC GGCTCCACTG GGTGGAGTTC 
CAGTCGAAGT TCTTCGGCGG AGCGGGCGAG TCGTTCAAGG CATTCAATTT CCCGACGTCA 
AACCAGAGGA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Vha100-3 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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