prom cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	prom
Entrez Gene ID	246493
Full Name	prominin
Synonyms	CG30164,CG30165,CG30166,CG42310,CG4556,Dmel\CG42310,Dmel_CG30164,Dmel_CG30165,Dmel_CG30166,Prom,eyc
General protein information	Preferred Names prominin Names CG42310 gene product from transcript CG42310-RB CG42310-PB CG42310-PC CG42310-PD CG42310-PE eyes closed prom-PB prom-PC prom-PD prom-PE
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 60C8-60C8

mRNA and Protein(s)

mRNA	Protein	Name
NM_001144281.2	NP_001137753.1	prominin, isoform B
NM_001144282.2	NP_001137754.1	prominin, isoform D
NM_001299914.1	NP_001286843.1	prominin, isoform E
NM_166671.2	NP_726444.2	prominin, isoform C

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the physiological role of Prominin is conserved between rhabdomeric and ciliated photoreceptor cells
Title: Cross species analysis of Prominin reveals a conserved cellular role in invertebrate and vertebrate photoreceptor cells.

The following prom gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the prom cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa11621	NM_166671.2 Latest version!	Drosophila melanogaster prominin (prom), transcript variant C, partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa12061	NM_001144281.2 Latest version!	Drosophila melanogaster prominin (prom), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa12062	NM_001144282.2 Latest version!	Drosophila melanogaster prominin (prom), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa22295	NM_001299914.1 Latest version!	Drosophila melanogaster prominin (prom), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

prom ( NM_166671.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726444.2 Drosophila melanogaster prominin (prom), transcript variant C, partial mRNA.

Price & Availability↑

CloneID	OFa11621
Clone ID Related Accession (Same CDS sequence)	NM_166671.2
Accession Version	NM_166671.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	prominin, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jan 26, 2009 this sequence version replaced NM_166671.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on the 3' end.

ATGGACCAGC GCAATCTAAC ACTGCCAACG CCAGCCGCAA CAACAATTAT GTCCTCGTCA 
TCAGCAGCGA TGTCGGCGGG CTTCGCATCC CTTGGAGGAC CCACCACGTC CGCCCTGACC 
GGATCCACAA TAGCATTAGC CACAACAACA ACAACGGCGC CGCCAACAAC GACGCCAGCC 
ACATTTTTTA CCTACAGCAT ACCAGGCATG GACATTGCGC CAGGACCATT TATATTCACC 
TACGTTAGTG AATTTTTATC GCTTATCACG CCCGAGGAAT TGCCGCTGGA TTCCATACGC 
GATGTTCTAC ACAAAAATAC CAGTCTGCTG GACTTTGCCT CCAATGCCAT TAAAATCGAG 
TCAGGCTTCA TTGCTCTGAT GAGCGTGTTC GCCATCCTGG CCCTGGTTCC CCTGATTGCC 
ACGTGTGCCT GGTGCTGCGG TCGTCGCTCC ACCCAGGAGG AGGTCGACCA CATACGGAAT 
GCACCCTGTC ACATCCGGGA CGAGTCGCTG GAGGAGAGCT TACGATGTCG CAAGAGCGCC 
GGACTGGTCA CCCTGTGGGT CGTCTTCATC CTGCTGACGC TGAGCGACTT CAGCATTTTT 
TACGCCAACA GTCGCTTGTC CGCCGGAATC GAGATGACGC CCGAGATGGT CAAGTCAGCC 
GTGCACGACG TGGAGGTCTT CCTGAAGGAC ACGCATCTAC AAATCAAGCA AAAACTGGAT 
AATGGCTTCC ACGTTTCCGT GGAGCGGGTC GTCAAAGACC TGGAAGACGT GGATGTTCTC 
CTTGGCGAGC CGATTCAAGC GGAAATATCC GCACACACTG GCCTTGAGCT GGCGTATGAT 
TCACTGACGA CACTTAGCCT GGCGAACGCG GAGCTCATCT ACCGCGTGCT CATGCTGCAG 
GAGACGGTGG GCAGGGCCCT GAAGCTCTCC CAGGAGGCGG CCGCCAGGAT GGAGGAGCTG 
CAGATCCAGC TGTCCGTGCT GCAGCGCCAG TGCACGTACC GCGACCGCCC GCTCTGCGAC 
ACGTTGCGTA TACGCAGCTT CGAGGAGAAC GGCGTCGTCG ACGCCCTGAA AACTCTGCAA 
GAGGACCAGA GCATCTTCCG TATGCGCTAC TTGGGCGAAA TTGAATTCGG TGCCACTGTC 
AAAAATCTGA CATCGGAAGT TGGCGTGTCA CGTTCCATTT TCAGCAATTT CCCGCAACAA 
GTTAAACATG ACACGGCTTA TGAGCGGAAC TATACATTGG AGCAGTTGGC CAGCATTCGC 
CACCGGACGA CTGCTAATGC CCAGCAACTG ACCTCTACGG TAAATGGACT GCTCCGGCGG 
CTGGAGGACA CCTGGTCATC CCTGCAGCCC GCCTATAACG AGCTGAAGGA GTGGGCAGAC 
GCCTACTGGA GTGTGGGCTG GATTGCCGGC ACGGTGATAG TGTGGGTAAT GCTCTTCATG 
CTGATGTCCT ACTGCTGCTT CCTCTGCGAA TCGAATGCCA AGTCCGGCGT GGTCTTCTTC 
ATCGCCGTGA TGCTCATCTG CTTGGCCAGC ATTTGCCTCA CCATTTTCGG GGTGTTTTCC 
CTGGCCGTGG GCGGTAATGC CGAGGTCTTT GTCTGCCGAT CACTCTACGA CGAGGACTAC 
AACATGCTGG GTAAGCTGCT GGACAAGCCG GGTTACGCAT ACGCCCGGGA GCCACAGACC 
GGCATCATCG GAGAGCTACT GCGTCCTGTC GGCGTCAATC GGTCGGTGGT CAACGTTGGT 
CTGGGCCAGG CCCTCAGGGG CTGCGAGCAA AATCAAGCAT CGTACAATGT CTTTCAATTG 
GATGCGTTCG TGAACACCAG CAAAATCGCC GACCTGAGGC AGTTCCCCAA AGTGTCCACC 
GCCATCGATG CCATCTCCGT GTCCGGCCGC ACGCTCCTGT CGCTCACGCA ATCCGTGCAG 
AACATTTTGG ATAACATGCT GCAGACGTCC TCGTTCAATT TGACCACGTA CCGCAGCAGC 
GTTGGTGCGC CCACGCCCGA AAAGGATTTG GCCACGTTCA TTGATCAGAT GCAGCGGGTG 
GCCTTGCAGA TTCAGGACGT GGCTACCTCC TCGCGTATGA CCACTCTGGG CAGCCGATCC 
AAGCGGCTGC AGGCCTCGAT TCTCCAGCCG CTGGAGAACC TGCAGAACGA GATTCTCTAC 
CACCTGACGG CCCTGGAGCT ACAGCGCGAT CCCTGGGCCA AGCAGGTCAA CCAGACTCTG 
AACCACCTCA AGAGCATCCA GAGCTACCTA GAGAACAAGG CCGGGGAGAT ATGCCACAAC 
ATGACACGGG ACTACAGGGA TCGCTTGAAG GCCTACTTGA CCACCAGTAA GGCAACCATG 
GAGTCCCAGC TGGGCGACAC TACGACCAAG TGCCGACCCT TCTACGACAC CTTCGACGCC 
AGCCGCACCT TCCTCTGCCG CAACATGGTG GATTTCATCA ACGGCCTCAC CTTCTTCATC 
TTCCTTACAC TGCTTCTCTG GGCTATTGGC ACTCCGGTGG GCCTCAACCT GATCACGGTT 
CAAACGCGGC TGAATGTGAT GCAGGCGGCT CAGGCGCGGA GCAAGGGCAG GCACAGGAGG 
AGCGACCGGA GTCCGGAGCA TGAGCAGAGG AGCGGAAGTC GCGGCCGGAA GAAGCAACGG 
ACGAGCAGTT CAGCGACAAG CGGGCGGAGC GGAAAGAGGG ACCAGAGCCG GGAGAGGAGC 
ACCAGCAGCA CGGCGAGACC GAAACCACCT TTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726444.2
CDS	507..3242
Translation	MDQRNLTLPTPAATTIMSSSSAAMSAGFASLGGPTTSALTGSTIALATTTTTAPPTTTPATFFTYSIPGMDIAPGPFIFTYVSEFLSLITPEELPLDSIRDVLHKNTSLLDFASNAIKIESGFIALMSVFAILALVPLIATCAWCCGRRSTQEEVDHIRNAPCHIRDESLEESLRCRKSAGLVTLWVVFILLTLSDFSIFYANSRLSAGIEMTPEMVKSAVHDVEVFLKDTHLQIKQKLDNGFHVSVERVVKDLEDVDVLLGEPIQAEISAHTGLELAYDSLTTLSLANAELIYRVLMLQETVGRALKLSQEAAARMEELQIQLSVLQRQCTYRDRPLCDTLRIRSFEENGVVDALKTLQEDQSIFRMRYLGEIEFGATVKNLTSEVGVSRSIFSNFPQQVKHDTAYERNYTLEQLASIRHRTTANAQQLTSTVNGLLRRLEDTWSSLQPAYNELKEWADAYWSVGWIAGTVIVWVMLFMLMSYCCFLCESNAKSGVVFFIAVMLICLASICLTIFGVFSLAVGGNAEVFVCRSLYDEDYNMLGKLLDKPGYAYAREPQTGIIGELLRPVGVNRSVVNVGLGQALRGCEQNQASYNVFQLDAFVNTSKIADLRQFPKVSTAIDAISVSGRTLLSLTQSVQNILDNMLQTSSFNLTTYRSSVGAPTPEKDLATFIDQMQRVALQIQDVATSSRMTTLGSRSKRLQASILQPLENLQNEILYHLTALELQRDPWAKQVNQTLNHLKSIQSYLENKAGEICHNMTRDYRDRLKAYLTTSKATMESQLGDTTTKCRPFYDTFDASRTFLCRNMVDFINGLTFFIFLTLLLWAIGTPVGLNLITVQTRLNVMQAAQARSKGRHRRSDRSPEHEQRSGSRGRKKQRTSSSATSGRSGKRDQSRERSTSSTARPKPPL

Target ORF information:

RefSeq Version	NM_166671.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster prominin (prom), transcript variant C, partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166671.2

ORF Insert Sequence:

ATGGACCAGC GCAATCTAAC ACTGCCAACG CCAGCCGCAA CAACAATTAT GTCCTCGTCA 
TCAGCAGCGA TGTCGGCGGG CTTCGCATCC CTTGGAGGAC CCACCACGTC CGCCCTGACC 
GGATCCACAA TAGCATTAGC CACAACAACA ACAACGGCGC CGCCAACAAC GACGCCAGCC 
ACATTTTTTA CCTACAGCAT ACCAGGCATG GACATTGCGC CAGGACCATT TATATTCACC 
TACGTTAGTG AATTTTTATC GCTTATCACG CCCGAGGAAT TGCCGCTGGA TTCCATACGC 
GATGTTCTAC ACAAAAATAC CAGTCTGCTG GACTTTGCCT CCAATGCCAT TAAAATCGAG 
TCAGGCTTCA TTGCTCTGAT GAGCGTGTTC GCCATCCTGG CCCTGGTTCC CCTGATTGCC 
ACGTGTGCCT GGTGCTGCGG TCGTCGCTCC ACCCAGGAGG AGGTCGACCA CATACGGAAT 
GCACCCTGTC ACATCCGGGA CGAGTCGCTG GAGGAGAGCT TACGATGTCG CAAGAGCGCC 
GGACTGGTCA CCCTGTGGGT CGTCTTCATC CTGCTGACGC TGAGCGACTT CAGCATTTTT 
TACGCCAACA GTCGCTTGTC CGCCGGAATC GAGATGACGC CCGAGATGGT CAAGTCAGCC 
GTGCACGACG TGGAGGTCTT CCTGAAGGAC ACGCATCTAC AAATCAAGCA AAAACTGGAT 
AATGGCTTCC ACGTTTCCGT GGAGCGGGTC GTCAAAGACC TGGAAGACGT GGATGTTCTC 
CTTGGCGAGC CGATTCAAGC GGAAATATCC GCACACACTG GCCTTGAGCT GGCGTATGAT 
TCACTGACGA CACTTAGCCT GGCGAACGCG GAGCTCATCT ACCGCGTGCT CATGCTGCAG 
GAGACGGTGG GCAGGGCCCT GAAGCTCTCC CAGGAGGCGG CCGCCAGGAT GGAGGAGCTG 
CAGATCCAGC TGTCCGTGCT GCAGCGCCAG TGCACGTACC GCGACCGCCC GCTCTGCGAC 
ACGTTGCGTA TACGCAGCTT CGAGGAGAAC GGCGTCGTCG ACGCCCTGAA AACTCTGCAA 
GAGGACCAGA GCATCTTCCG TATGCGCTAC TTGGGCGAAA TTGAATTCGG TGCCACTGTC 
AAAAATCTGA CATCGGAAGT TGGCGTGTCA CGTTCCATTT TCAGCAATTT CCCGCAACAA 
GTTAAACATG ACACGGCTTA TGAGCGGAAC TATACATTGG AGCAGTTGGC CAGCATTCGC 
CACCGGACGA CTGCTAATGC CCAGCAACTG ACCTCTACGG TAAATGGACT GCTCCGGCGG 
CTGGAGGACA CCTGGTCATC CCTGCAGCCC GCCTATAACG AGCTGAAGGA GTGGGCAGAC 
GCCTACTGGA GTGTGGGCTG GATTGCCGGC ACGGTGATAG TGTGGGTAAT GCTCTTCATG 
CTGATGTCCT ACTGCTGCTT CCTCTGCGAA TCGAATGCCA AGTCCGGCGT GGTCTTCTTC 
ATCGCCGTGA TGCTCATCTG CTTGGCCAGC ATTTGCCTCA CCATTTTCGG GGTGTTTTCC 
CTGGCCGTGG GCGGTAATGC CGAGGTCTTT GTCTGCCGAT CACTCTACGA CGAGGACTAC 
AACATGCTGG GTAAGCTGCT GGACAAGCCG GGTTACGCAT ACGCCCGGGA GCCACAGACC 
GGCATCATCG GAGAGCTACT GCGTCCTGTC GGCGTCAATC GGTCGGTGGT CAACGTTGGT 
CTGGGCCAGG CCCTCAGGGG CTGCGAGCAA AATCAAGCAT CGTACAATGT CTTTCAATTG 
GATGCGTTCG TGAACACCAG CAAAATCGCC GACCTGAGGC AGTTCCCCAA AGTGTCCACC 
GCCATCGATG CCATCTCCGT GTCCGGCCGC ACGCTCCTGT CGCTCACGCA ATCCGTGCAG 
AACATTTTGG ATAACATGCT GCAGACGTCC TCGTTCAATT TGACCACGTA CCGCAGCAGC 
GTTGGTGCGC CCACGCCCGA AAAGGATTTG GCCACGTTCA TTGATCAGAT GCAGCGGGTG 
GCCTTGCAGA TTCAGGACGT GGCTACCTCC TCGCGTATGA CCACTCTGGG CAGCCGATCC 
AAGCGGCTGC AGGCCTCGAT TCTCCAGCCG CTGGAGAACC TGCAGAACGA GATTCTCTAC 
CACCTGACGG CCCTGGAGCT ACAGCGCGAT CCCTGGGCCA AGCAGGTCAA CCAGACTCTG 
AACCACCTCA AGAGCATCCA GAGCTACCTA GAGAACAAGG CCGGGGAGAT ATGCCACAAC 
ATGACACGGG ACTACAGGGA TCGCTTGAAG GCCTACTTGA CCACCAGTAA GGCAACCATG 
GAGTCCCAGC TGGGCGACAC TACGACCAAG TGCCGACCCT TCTACGACAC CTTCGACGCC 
AGCCGCACCT TCCTCTGCCG CAACATGGTG GATTTCATCA ACGGCCTCAC CTTCTTCATC 
TTCCTTACAC TGCTTCTCTG GGCTATTGGC ACTCCGGTGG GCCTCAACCT GATCACGGTT 
CAAACGCGGC TGAATGTGAT GCAGGCGGCT CAGGCGCGGA GCAAGGGCAG GCACAGGAGG 
AGCGACCGGA GTCCGGAGCA TGAGCAGAGG AGCGGAAGTC GCGGCCGGAA GAAGCAACGG 
ACGAGCAGTT CAGCGACAAG CGGGCGGAGC GGAAAGAGGG ACCAGAGCCG GGAGAGGAGC 
ACCAGCAGCA CGGCGAGACC GAAACCACCT TTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prom ( NM_001144281.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137753.1 Drosophila melanogaster prominin (prom), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa12061
Clone ID Related Accession (Same CDS sequence)	NM_001144281.2
Accession Version	NM_001144281.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	prominin, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001144281.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACCAGC GCAATCTAAC ACTGCCAACG CCAGCCGCAA CAACAATTAT GTCCTCGTCA 
TCAGCAGCGA TGTCGGCGGG CTTCGCATCC CTTGGAGGAC CCACCACGTC CGCCCTGACC 
GGATCCACAA TAGCATTAGC CACAACAACA ACAACGGCGC CGCCAACAAC GACGCCAGCC 
ACATTTTTTA CCTACAGCAT ACCAGGCATG GACATTGCGC CAGGACCATT TATATTCACC 
TACGTTAGTG AATTTTTATC GCTTATCACG CCCGAGGAAT TGCCGCTGGA TTCCATACGC 
GATGTTCTAC ACAAAAATAC CAGTCTGCTG GACTTTGCCT CCAATGCCAT TAAAATCGAG 
TCAGGCTTCA TTGCTCTGAT GAGCGTGTTC GCCATCCTGG CCCTGGTTCC CCTGATTGCC 
ACGTGTGCCT GGTGCTGCGG TCGTCGCTCC ACCCAGGAGG AGGTCGACCA CATACGGAAT 
GCACCCTGTC ACATCCGGGA CGAGTCGCTG GAGGAGAGCT TACGATGTCG CAAGAGCGCC 
GGACTGGTCA CCCTGTGGGT CGTCTTCATC CTGCTGACGC TGAGCGACTT CAGCATTTTT 
TACGCCAACA GTCGCTTGTC CGCCGGAATC GAGATGACGC CCGAGATGGT CAAGTCAGCC 
GTGCACGACG TGGAGGTCTT CCTGAAGGAC ACGCATCTAC AAATCAAGCA AAAACTGGAT 
AATGGCTTCC ACGTTTCCGT GGAGCGGGTC GTCAAAGACC TGGAAGACGT GGATGTTCTC 
CTTGGCGAGC CGATTCAAGC GGAAATATCC GCACACACTG GCCTTGAGCT GGCGTATGAT 
TCACTGACGA CACTTAGCCT GGCGAACGCG GAGCTCATCT ACCGCGTGCT CATGCTGCAG 
GAGACGGTGG GCAGGGCCCT GAAGCTCTCC CAGGAGGCGG CCGCCAGGAT GGAGGAGCTG 
CAGATCCAGC TGTCCGTGCT GCAGCGCCAG TGCACGTACC GCGACCGCCC GCTCTGCGAC 
ACGTTGCGTA TACGCAGCTT CGAGGAGAAC GGCGTCGTCG ACGCCCTGAA AACTCTGCAA 
GAGGACCAGA GCATCTTCCG TATGCGCTAC TTGGGCGAAA TTGAATTCGG TGCCACTGTC 
AAAAATCTGA CATCGGAAGT TGGCGTGTCA CGTTCCATTT TCAGCAATTT CCCGCAACAA 
GTTAAACATG ACACGGCTTA TGAGCGGAAC TATACATTGG AGCAGTTGGC CAGCATTCGC 
CACCGGACGA CTGCTAATGC CCAGCAACTG ACCTCTACGG TAAATGGACT GCTCCGGCGG 
CTGGAGGACA CCTGGTCATC CCTGCAGCCC GCCTATAACG AGCTGAAGGA GTGGGCAGAC 
GCCTACTGGA GTGTGGGCTG GATTGCCGGC ACGGTGATAG TGTGGGTAAT GCTCTTCATG 
CTGATGTCCT ACTGCTGCTT CCTCTGCGAA TCGAATGCCA AGTCCGGCGT GGTCTTCTTC 
ATCGCCGTGA TGCTCATCTG CTTGGCCAGC ATTTGCCTCA CCATTTTCGG GGTGTTTTCC 
CTGGCCGTGG GCGGTAATGC CGAGGTCTTT GTCTGCCGAT CACTCTACGA CGAGGACTAC 
AACATGCTGG GTAAGCTGCT GGACAAGCCG GGTTACGCAT ACGCCCGGGA GCCACAGACC 
GGCATCATCG GAGAGCTACT GCGTCCTGTC GGCGTCAATC GGTCGGTGGT CAACGTTGGT 
CTGGGCCAGG CCCTCAGGGG CTGCGAGCAA AATCAAGCAT CGTACAATGT CTTTCAATTG 
GATGCGTTCG TGAACACCAG CAAAATCGCC GACCTGAGGC AGTTCCCCAA AGTGTCCACC 
GCCATCGATG CCATCTCCGT GTCCGGCCGC ACGCTCCTGT CGCTCACGCA ATCCGTGCAG 
AACATTTTGG ATAACATGCT GCAGACGTCC TCGTTCAATT TGACCACGTA CCGCAGCAGC 
GTTGGTGCGC CCACGCCCGA AAAGGATTTG GCCACGTTCA TTGATCAGAT GCAGCGGGTG 
GCCTTGCAGA TTCAGGACGT GGCTACCTCC TCGCGTATGA CCACTCTGGG CAGCCGATCC 
AAGCGGCTGC AGGCCTCGAT TCTCCAGCCG CTGGAGAACC TGCAGAACGA GATTCTCTAC 
CACCTGACGG CCCTGGAGCT ACAGCGCGAT CCCTGGGCCA AGCAGGTCAA CCAGACTCTG 
AACCACCTCA AGAGCATCCA GAGCTACCTA GAGAACAAGG CCGGGGAGAT ATGCCACAAC 
ATGACACGGG ACTACAGGGA TCGCTTGAAG GCCTACTTGA CCACCAGTAA GGCAACCATG 
GAGTCCCAGC TGGGCGACAC TACGACCAAG TGCCGACCCT TCTACGACAC CTTCGACGCC 
AGCCGCACCT TCCTCTGCCG CAACATGGTG GATTTCATCA ACGGCCTCAC CTTCTTCATC 
TTCCTTACAC TGCTTCTCTG GGCTATTGGC ACTCCGGTGG GCCTCAACCT GATCACGGTT 
CAAACGCGGC TGAATGTGAT GCAGGCGGCT CAGGCGCGGA GCAAGGGCAG GCACAGGAGG 
AGCGACCGGA GTCCGGAGCA TGAGCAGAGG AGCGGAAGTC GCGGCCGGAA GAAGCAACGG 
ACGAGCAGTT CAGCGACAAG CGGGCGGAGC GGAAAGAGGG ACCAGAGCCG GGAGAGGAGC 
ACCAGCAGCA CGGCGAGACC GAAACCACCT TTAAGACGGA CTGCCACTGA AGAAACACTT 
GATCTGGCAG ACGAAGAATC CACCCCGTCA AGACAGCAGC AGCAGCAGCC GCCGCCACAG 
CGACGCCAGG AATCGGACAG GGAGCGGGTG CGGGAGCGGG ATCAGCGAAG TCGGGAGCGG 
GAATTGAGCA GGGGCCGTGA CGCGTCCGCC ATGAACACGC CAGTGCGCAG CCGCAGTCGC 
CAGCAACTCC AGCGAACCGG CACCGATGAG TTCGACTTGG ACGACAGCGA TGCATACGGT 
GCTGGAGGCC ATTTGGATGC GGACGTGGCG GCCGCGCGAC GCACTCCGCC CAGGGGCGCA 
CCTCCACCCC CTCCTCCGCC AGCGGCCGGC TTGAACAGTG ATCGCACGGT GCGCTGGAAC 
GAGGACGAAA CCGAGGACTT TGACTTCGAG GAGGTCGACG CGCCGAAGAG TCCCATGTAT 
CTGAGCCACA ATCTCCAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137753.1
CDS	507..3707
Translation	MDQRNLTLPTPAATTIMSSSSAAMSAGFASLGGPTTSALTGSTIALATTTTTAPPTTTPATFFTYSIPGMDIAPGPFIFTYVSEFLSLITPEELPLDSIRDVLHKNTSLLDFASNAIKIESGFIALMSVFAILALVPLIATCAWCCGRRSTQEEVDHIRNAPCHIRDESLEESLRCRKSAGLVTLWVVFILLTLSDFSIFYANSRLSAGIEMTPEMVKSAVHDVEVFLKDTHLQIKQKLDNGFHVSVERVVKDLEDVDVLLGEPIQAEISAHTGLELAYDSLTTLSLANAELIYRVLMLQETVGRALKLSQEAAARMEELQIQLSVLQRQCTYRDRPLCDTLRIRSFEENGVVDALKTLQEDQSIFRMRYLGEIEFGATVKNLTSEVGVSRSIFSNFPQQVKHDTAYERNYTLEQLASIRHRTTANAQQLTSTVNGLLRRLEDTWSSLQPAYNELKEWADAYWSVGWIAGTVIVWVMLFMLMSYCCFLCESNAKSGVVFFIAVMLICLASICLTIFGVFSLAVGGNAEVFVCRSLYDEDYNMLGKLLDKPGYAYAREPQTGIIGELLRPVGVNRSVVNVGLGQALRGCEQNQASYNVFQLDAFVNTSKIADLRQFPKVSTAIDAISVSGRTLLSLTQSVQNILDNMLQTSSFNLTTYRSSVGAPTPEKDLATFIDQMQRVALQIQDVATSSRMTTLGSRSKRLQASILQPLENLQNEILYHLTALELQRDPWAKQVNQTLNHLKSIQSYLENKAGEICHNMTRDYRDRLKAYLTTSKATMESQLGDTTTKCRPFYDTFDASRTFLCRNMVDFINGLTFFIFLTLLLWAIGTPVGLNLITVQTRLNVMQAAQARSKGRHRRSDRSPEHEQRSGSRGRKKQRTSSSATSGRSGKRDQSRERSTSSTARPKPPLRRTATEETLDLADEESTPSRQQQQQPPPQRRQESDRERVRERDQRSRERELSRGRDASAMNTPVRSRSRQQLQRTGTDEFDLDDSDAYGAGGHLDADVAAARRTPPRGAPPPPPPPAAGLNSDRTVRWNEDETEDFDFEEVDAPKSPMYLSHNLQ

Target ORF information:

RefSeq Version	NM_001144281.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster prominin (prom), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144281.2

ORF Insert Sequence:

ATGGACCAGC GCAATCTAAC ACTGCCAACG CCAGCCGCAA CAACAATTAT GTCCTCGTCA 
TCAGCAGCGA TGTCGGCGGG CTTCGCATCC CTTGGAGGAC CCACCACGTC CGCCCTGACC 
GGATCCACAA TAGCATTAGC CACAACAACA ACAACGGCGC CGCCAACAAC GACGCCAGCC 
ACATTTTTTA CCTACAGCAT ACCAGGCATG GACATTGCGC CAGGACCATT TATATTCACC 
TACGTTAGTG AATTTTTATC GCTTATCACG CCCGAGGAAT TGCCGCTGGA TTCCATACGC 
GATGTTCTAC ACAAAAATAC CAGTCTGCTG GACTTTGCCT CCAATGCCAT TAAAATCGAG 
TCAGGCTTCA TTGCTCTGAT GAGCGTGTTC GCCATCCTGG CCCTGGTTCC CCTGATTGCC 
ACGTGTGCCT GGTGCTGCGG TCGTCGCTCC ACCCAGGAGG AGGTCGACCA CATACGGAAT 
GCACCCTGTC ACATCCGGGA CGAGTCGCTG GAGGAGAGCT TACGATGTCG CAAGAGCGCC 
GGACTGGTCA CCCTGTGGGT CGTCTTCATC CTGCTGACGC TGAGCGACTT CAGCATTTTT 
TACGCCAACA GTCGCTTGTC CGCCGGAATC GAGATGACGC CCGAGATGGT CAAGTCAGCC 
GTGCACGACG TGGAGGTCTT CCTGAAGGAC ACGCATCTAC AAATCAAGCA AAAACTGGAT 
AATGGCTTCC ACGTTTCCGT GGAGCGGGTC GTCAAAGACC TGGAAGACGT GGATGTTCTC 
CTTGGCGAGC CGATTCAAGC GGAAATATCC GCACACACTG GCCTTGAGCT GGCGTATGAT 
TCACTGACGA CACTTAGCCT GGCGAACGCG GAGCTCATCT ACCGCGTGCT CATGCTGCAG 
GAGACGGTGG GCAGGGCCCT GAAGCTCTCC CAGGAGGCGG CCGCCAGGAT GGAGGAGCTG 
CAGATCCAGC TGTCCGTGCT GCAGCGCCAG TGCACGTACC GCGACCGCCC GCTCTGCGAC 
ACGTTGCGTA TACGCAGCTT CGAGGAGAAC GGCGTCGTCG ACGCCCTGAA AACTCTGCAA 
GAGGACCAGA GCATCTTCCG TATGCGCTAC TTGGGCGAAA TTGAATTCGG TGCCACTGTC 
AAAAATCTGA CATCGGAAGT TGGCGTGTCA CGTTCCATTT TCAGCAATTT CCCGCAACAA 
GTTAAACATG ACACGGCTTA TGAGCGGAAC TATACATTGG AGCAGTTGGC CAGCATTCGC 
CACCGGACGA CTGCTAATGC CCAGCAACTG ACCTCTACGG TAAATGGACT GCTCCGGCGG 
CTGGAGGACA CCTGGTCATC CCTGCAGCCC GCCTATAACG AGCTGAAGGA GTGGGCAGAC 
GCCTACTGGA GTGTGGGCTG GATTGCCGGC ACGGTGATAG TGTGGGTAAT GCTCTTCATG 
CTGATGTCCT ACTGCTGCTT CCTCTGCGAA TCGAATGCCA AGTCCGGCGT GGTCTTCTTC 
ATCGCCGTGA TGCTCATCTG CTTGGCCAGC ATTTGCCTCA CCATTTTCGG GGTGTTTTCC 
CTGGCCGTGG GCGGTAATGC CGAGGTCTTT GTCTGCCGAT CACTCTACGA CGAGGACTAC 
AACATGCTGG GTAAGCTGCT GGACAAGCCG GGTTACGCAT ACGCCCGGGA GCCACAGACC 
GGCATCATCG GAGAGCTACT GCGTCCTGTC GGCGTCAATC GGTCGGTGGT CAACGTTGGT 
CTGGGCCAGG CCCTCAGGGG CTGCGAGCAA AATCAAGCAT CGTACAATGT CTTTCAATTG 
GATGCGTTCG TGAACACCAG CAAAATCGCC GACCTGAGGC AGTTCCCCAA AGTGTCCACC 
GCCATCGATG CCATCTCCGT GTCCGGCCGC ACGCTCCTGT CGCTCACGCA ATCCGTGCAG 
AACATTTTGG ATAACATGCT GCAGACGTCC TCGTTCAATT TGACCACGTA CCGCAGCAGC 
GTTGGTGCGC CCACGCCCGA AAAGGATTTG GCCACGTTCA TTGATCAGAT GCAGCGGGTG 
GCCTTGCAGA TTCAGGACGT GGCTACCTCC TCGCGTATGA CCACTCTGGG CAGCCGATCC 
AAGCGGCTGC AGGCCTCGAT TCTCCAGCCG CTGGAGAACC TGCAGAACGA GATTCTCTAC 
CACCTGACGG CCCTGGAGCT ACAGCGCGAT CCCTGGGCCA AGCAGGTCAA CCAGACTCTG 
AACCACCTCA AGAGCATCCA GAGCTACCTA GAGAACAAGG CCGGGGAGAT ATGCCACAAC 
ATGACACGGG ACTACAGGGA TCGCTTGAAG GCCTACTTGA CCACCAGTAA GGCAACCATG 
GAGTCCCAGC TGGGCGACAC TACGACCAAG TGCCGACCCT TCTACGACAC CTTCGACGCC 
AGCCGCACCT TCCTCTGCCG CAACATGGTG GATTTCATCA ACGGCCTCAC CTTCTTCATC 
TTCCTTACAC TGCTTCTCTG GGCTATTGGC ACTCCGGTGG GCCTCAACCT GATCACGGTT 
CAAACGCGGC TGAATGTGAT GCAGGCGGCT CAGGCGCGGA GCAAGGGCAG GCACAGGAGG 
AGCGACCGGA GTCCGGAGCA TGAGCAGAGG AGCGGAAGTC GCGGCCGGAA GAAGCAACGG 
ACGAGCAGTT CAGCGACAAG CGGGCGGAGC GGAAAGAGGG ACCAGAGCCG GGAGAGGAGC 
ACCAGCAGCA CGGCGAGACC GAAACCACCT TTAAGACGGA CTGCCACTGA AGAAACACTT 
GATCTGGCAG ACGAAGAATC CACCCCGTCA AGACAGCAGC AGCAGCAGCC GCCGCCACAG 
CGACGCCAGG AATCGGACAG GGAGCGGGTG CGGGAGCGGG ATCAGCGAAG TCGGGAGCGG 
GAATTGAGCA GGGGCCGTGA CGCGTCCGCC ATGAACACGC CAGTGCGCAG CCGCAGTCGC 
CAGCAACTCC AGCGAACCGG CACCGATGAG TTCGACTTGG ACGACAGCGA TGCATACGGT 
GCTGGAGGCC ATTTGGATGC GGACGTGGCG GCCGCGCGAC GCACTCCGCC CAGGGGCGCA 
CCTCCACCCC CTCCTCCGCC AGCGGCCGGC TTGAACAGTG ATCGCACGGT GCGCTGGAAC 
GAGGACGAAA CCGAGGACTT TGACTTCGAG GAGGTCGACG CGCCGAAGAG TCCCATGTAT 
CTGAGCCACA ATCTCCAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prom ( NM_001144282.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001137754.1 Drosophila melanogaster prominin (prom), transcript variant D, mRNA.

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CloneID	OFa12062
Clone ID Related Accession (Same CDS sequence)	NM_001144282.2
Accession Version	NM_001144282.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	prominin, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001144282.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACCAGC GCAATCTAAC ACTGCCAACG CCAGCCGCAA CAACAATTAT GTCCTCGTCA 
TCAGCAGCGA TGTCGGCGGG CTTCGCATCC CTTGGAGGAC CCACCACGTC CGCCCTGACC 
GGATCCACAA TAGCATTAGC CACAACAACA ACAACGGCGC CGCCAACAAC GACGCCAGCC 
ACATTTTTTA CCTACAGCAT ACCAGGCATG GACATTGCGC CAGGACCATT TATATTCACC 
TACGTTAGTG AATTTTTATC GCTTATCACG CCCGAGGAAT TGCCGCTGGA TTCCATACGC 
GATGTTCTAC ACAAAAATAC CAGTCTGCTG GACTTTGCCT CCAATGCCAT TAAAATCGAG 
TCAGGCTTCA TTGCTCTGAT GAGCGTGTTC GCCATCCTGG CCCTGGTTCC CCTGATTGCC 
ACGTGTGCCT GGTGCTGCGG TCGTCGCTCC ACCCAGGAGG AGGTCGACCA CATACGGAAT 
GCACCCTGTC ACATCCGGGA CGAGTCGCTG GAGGAGAGCT TACGATGTCG CAAGAGCGCC 
GGACTGGTCA CCCTGTGGGT CGTCTTCATC CTGCTGACGC TGAGCGACTT CAGCATTTTT 
TACGCCAACA GTCGCTTGTC CGCCGGAATC GAGATGACGC CCGAGATGGT CAAGTCAGCC 
GTGCACGACG TGGAGGTCTT CCTGAAGGAC ACGCATCTAC AAATCAAGCA AAAACTGGAT 
AATGGCTTCC ACGTTTCCGT GGAGCGGGTC GTCAAAGACC TGGAAGACGT GGATGTTCTC 
CTTGGCGAGC CGATTCAAGC GGAAATATCC GCACACACTG GCCTTGAGCT GGCGTATGAT 
TCACTGACGA CACTTAGCCT GGCGAACGCG GAGCTCATCT ACCGCGTGCT CATGCTGCAG 
GAGACGGTGG GCAGGGCCCT GAAGCTCTCC CAGGAGGCGG CCGCCAGGAT GGAGGAGCTG 
CAGATCCAGC TGTCCGTGCT GCAGCGCCAG TGCACGTACC GCGACCGCCC GCTCTGCGAC 
ACGTTGCGTA TACGCAGCTT CGAGGAGAAC GGCGTCGTCG ACGCCCTGAA AACTCTGCAA 
GAGGACCAGA GCATCTTCCG TATGCGCTAC TTGGGCGAAA TTGAATTCGG TGCCACTGTC 
AAAAATCTGA CATCGGAAGT TGGCGTGTCA CGTTCCATTT TCAGCAATTT CCCGCAACAA 
GTTAAACATG ACACGGCTTA TGAGCGGAAC TATACATTGG AGCAGTTGGC CAGCATTCGC 
CACCGGACGA CTGCTAATGC CCAGCAACTG ACCTCTACGG TAAATGGACT GCTCCGGCGG 
CTGGAGGACA CCTGGTCATC CCTGCAGCCC GCCTATAACG AGCTGAAGGA GTGGGCAGAC 
GCCTACTGGA GTGTGGGCTG GATTGCCGGC ACGGTGATAG TGTGGGTAAT GCTCTTCATG 
CTGATGTCCT ACTGCTGCTT CCTCTGCGAA TCGAATGCCA AGTCCGGCGT GGTCTTCTTC 
ATCGCCGTGA TGCTCATCTG CTTGGCCAGC ATTTGCCTCA CCATTTTCGG GGTGTTTTCC 
CTGGCCGTGG GCGGTAATGC CGAGGTCTTT GTCTGCCGAT CACTCTACGA CGAGGACTAC 
AACATGCTGG GTAAGCTGCT GGACAAGCCG GGTTACGCAT ACGCCCGGGA GCCACAGACC 
GGCATCATCG GAGAGCTACT GCGTCCTGTC GGCGTCAATC GGTCGGTGGT CAACGTTGGT 
CTGGGCCAGG CCCTCAGGGG CTGCGAGCAA AATCAAGCAT CGTACAATGT CTTTCAATTG 
GATGCGTTCG TGAACACCAG CAAAATCGCC GACCTGAGGC AGTTCCCCAA AGTGTCCACC 
GCCATCGATG CCATCTCCGT GTCCGGCCGC ACGCTCCTGT CGCTCACGCA ATCCGTGCAG 
AACATTTTGG ATAACATGCT GCAGACGTCC TCGTTCAATT TGACCACGTA CCGCAGCAGC 
GTTGGTGCGC CCACGCCCGA AAAGGATTTG GCCACGTTCA TTGATCAGAT GCAGCGGGTG 
GCCTTGCAGA TTCAGGACGT GGCTACCTCC TCGCGTATGA CCACTCTGGG CAGCCGATCC 
AAGCGGCTGC AGGCCTCGAT TCTCCAGCCG CTGGAGAACC TGCAGAACGA GATTCTCTAC 
CACCTGACGG CCCTGGAGCT ACAGCGCGAT CCCTGGGCCA AGCAGGTCAA CCAGACTCTG 
AACCACCTCA AGAGCATCCA GAGCTACCTA GAGAACAAGG CCGGGGAGAT ATGCCACAAC 
ATGACACGGG ACTACAGGGA TCGCTTGAAG GCCTACTTGA CCACCAGTAA GGCAACCATG 
GAGTCCCAGC TGGGCGACAC TACGACCAAG TGCCGACCCT TCTACGACAC CTTCGACGCC 
AGCCGCACCT TCCTCTGCCG CAACATGGTG GATTTCATCA ACGGCCTCAC CTTCTTCATC 
TTCCTTACAC TGCTTCTCTG GGCTATTGGC ACTCCGGTGG GCCTCAACCT GATCACGGTT 
CAAACGCGGC TGAATGTGAT GCAGGCGGCT CAGGCGCGGA GCAAGGGCAG GCACAGGAGG 
AGCGACCGGA GTCCGGAGCA TGAGCAGAGG AGCGGAAGTC GCGGCCGGAA GAAGCAACGG 
ACGAGCAGTT CAGCGACAAG CGGGCGGAGC GGAAAGAGGG ACCAGAGCCG GGAGAGGAGC 
ACCAGCAGCA CGGCGAGACC GAAACCACCT TTAAGACGGA CTGCCACTGA AGAAACACTT 
GATCTGGCAG ACGAAGAATC CACCCCGTCA AGACAGCAGC AGCAGCAGCC GCCGCCACAG 
CGACGCCAGG AATCGGACAG GGAGCGGGTG CGGGAGCGGG ATCAGCGAAG TCGGGAGCGG 
GAATTGAGCA GGGGCCGTGA CGCGTCCGCC ATGAACACGC CAGTGCGCAG CCGCAGTCGC 
CAGCAACTGC GCCATGACCC CGAGGATGAC AATTGGGGCT CGTCCAGCGG ACCCAGTAGG 
GCCAACTCTC GAGAGCGAGA TCGCGAGAGC AGTCAGACTA GAAAGATACT CAATATTTGG 
CAATCGCGCA ATAAGGCCAA GACGCCCCTT CGCCGTCAAG GCACCGAAGA GTTCGACTTT 
GAGGATGCCC ACGACGACAG AGGCTGGCAA GCCCAGACCC AAAACCAGGT TCAGAACCAA 
AGCCGCGCTC ATAACCAGTC CAGTGTCAGC CCCGAACAAA GGCCCGATCC GCGCAGCCAT 
CGCTCCAATC TCCGTACTAA GCCCAAGTTA CGTAGTACCA ATGCGGATAG AGCTAGCAGT 
GAAGCGATCC GGCGACCCAT AACTCCCGTG CTCAGCGTAA ATTCGGACAT TCCGGCCGCC 
ATTCCGATGC CCCGGACGCC GATGCGTCTC AAGAGCAAGG TGTCGTTGAC CGCCCGAGCT 
GTGCAGGACA TTGTCAACAA GCGGAATCAG CGCCTCCAGC GAACCGGCAC CGATGAGTTC 
GACTTGGACG ACAGCGATGC ATACGGTGCT GGAGGCCATT TGGATGCGGA CGTGGCGGCC 
GCGCGACGCA CTCCGCCCAG GGGCGCACCT CCACCCCCTC CTCCGCCAGC GGCCGGCTTG 
AACAGTGATC GCACGGTGCG CTGGAACGAG GACGAAACCG AGGACTTTGA CTTCGAGGAG 
GTCGACGCGC CGAAGAGTCC CATGTATCTG AGCCACAATC TCCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137754.1
CDS	507..4214
Translation	MDQRNLTLPTPAATTIMSSSSAAMSAGFASLGGPTTSALTGSTIALATTTTTAPPTTTPATFFTYSIPGMDIAPGPFIFTYVSEFLSLITPEELPLDSIRDVLHKNTSLLDFASNAIKIESGFIALMSVFAILALVPLIATCAWCCGRRSTQEEVDHIRNAPCHIRDESLEESLRCRKSAGLVTLWVVFILLTLSDFSIFYANSRLSAGIEMTPEMVKSAVHDVEVFLKDTHLQIKQKLDNGFHVSVERVVKDLEDVDVLLGEPIQAEISAHTGLELAYDSLTTLSLANAELIYRVLMLQETVGRALKLSQEAAARMEELQIQLSVLQRQCTYRDRPLCDTLRIRSFEENGVVDALKTLQEDQSIFRMRYLGEIEFGATVKNLTSEVGVSRSIFSNFPQQVKHDTAYERNYTLEQLASIRHRTTANAQQLTSTVNGLLRRLEDTWSSLQPAYNELKEWADAYWSVGWIAGTVIVWVMLFMLMSYCCFLCESNAKSGVVFFIAVMLICLASICLTIFGVFSLAVGGNAEVFVCRSLYDEDYNMLGKLLDKPGYAYAREPQTGIIGELLRPVGVNRSVVNVGLGQALRGCEQNQASYNVFQLDAFVNTSKIADLRQFPKVSTAIDAISVSGRTLLSLTQSVQNILDNMLQTSSFNLTTYRSSVGAPTPEKDLATFIDQMQRVALQIQDVATSSRMTTLGSRSKRLQASILQPLENLQNEILYHLTALELQRDPWAKQVNQTLNHLKSIQSYLENKAGEICHNMTRDYRDRLKAYLTTSKATMESQLGDTTTKCRPFYDTFDASRTFLCRNMVDFINGLTFFIFLTLLLWAIGTPVGLNLITVQTRLNVMQAAQARSKGRHRRSDRSPEHEQRSGSRGRKKQRTSSSATSGRSGKRDQSRERSTSSTARPKPPLRRTATEETLDLADEESTPSRQQQQQPPPQRRQESDRERVRERDQRSRERELSRGRDASAMNTPVRSRSRQQLRHDPEDDNWGSSSGPSRANSRERDRESSQTRKILNIWQSRNKAKTPLRRQGTEEFDFEDAHDDRGWQAQTQNQVQNQSRAHNQSSVSPEQRPDPRSHRSNLRTKPKLRSTNADRASSEAIRRPITPVLSVNSDIPAAIPMPRTPMRLKSKVSLTARAVQDIVNKRNQRLQRTGTDEFDLDDSDAYGAGGHLDADVAAARRTPPRGAPPPPPPPAAGLNSDRTVRWNEDETEDFDFEEVDAPKSPMYLSHNLQ

Target ORF information:

RefSeq Version	NM_001144282.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster prominin (prom), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144282.2

ORF Insert Sequence:

ATGGACCAGC GCAATCTAAC ACTGCCAACG CCAGCCGCAA CAACAATTAT GTCCTCGTCA 
TCAGCAGCGA TGTCGGCGGG CTTCGCATCC CTTGGAGGAC CCACCACGTC CGCCCTGACC 
GGATCCACAA TAGCATTAGC CACAACAACA ACAACGGCGC CGCCAACAAC GACGCCAGCC 
ACATTTTTTA CCTACAGCAT ACCAGGCATG GACATTGCGC CAGGACCATT TATATTCACC 
TACGTTAGTG AATTTTTATC GCTTATCACG CCCGAGGAAT TGCCGCTGGA TTCCATACGC 
GATGTTCTAC ACAAAAATAC CAGTCTGCTG GACTTTGCCT CCAATGCCAT TAAAATCGAG 
TCAGGCTTCA TTGCTCTGAT GAGCGTGTTC GCCATCCTGG CCCTGGTTCC CCTGATTGCC 
ACGTGTGCCT GGTGCTGCGG TCGTCGCTCC ACCCAGGAGG AGGTCGACCA CATACGGAAT 
GCACCCTGTC ACATCCGGGA CGAGTCGCTG GAGGAGAGCT TACGATGTCG CAAGAGCGCC 
GGACTGGTCA CCCTGTGGGT CGTCTTCATC CTGCTGACGC TGAGCGACTT CAGCATTTTT 
TACGCCAACA GTCGCTTGTC CGCCGGAATC GAGATGACGC CCGAGATGGT CAAGTCAGCC 
GTGCACGACG TGGAGGTCTT CCTGAAGGAC ACGCATCTAC AAATCAAGCA AAAACTGGAT 
AATGGCTTCC ACGTTTCCGT GGAGCGGGTC GTCAAAGACC TGGAAGACGT GGATGTTCTC 
CTTGGCGAGC CGATTCAAGC GGAAATATCC GCACACACTG GCCTTGAGCT GGCGTATGAT 
TCACTGACGA CACTTAGCCT GGCGAACGCG GAGCTCATCT ACCGCGTGCT CATGCTGCAG 
GAGACGGTGG GCAGGGCCCT GAAGCTCTCC CAGGAGGCGG CCGCCAGGAT GGAGGAGCTG 
CAGATCCAGC TGTCCGTGCT GCAGCGCCAG TGCACGTACC GCGACCGCCC GCTCTGCGAC 
ACGTTGCGTA TACGCAGCTT CGAGGAGAAC GGCGTCGTCG ACGCCCTGAA AACTCTGCAA 
GAGGACCAGA GCATCTTCCG TATGCGCTAC TTGGGCGAAA TTGAATTCGG TGCCACTGTC 
AAAAATCTGA CATCGGAAGT TGGCGTGTCA CGTTCCATTT TCAGCAATTT CCCGCAACAA 
GTTAAACATG ACACGGCTTA TGAGCGGAAC TATACATTGG AGCAGTTGGC CAGCATTCGC 
CACCGGACGA CTGCTAATGC CCAGCAACTG ACCTCTACGG TAAATGGACT GCTCCGGCGG 
CTGGAGGACA CCTGGTCATC CCTGCAGCCC GCCTATAACG AGCTGAAGGA GTGGGCAGAC 
GCCTACTGGA GTGTGGGCTG GATTGCCGGC ACGGTGATAG TGTGGGTAAT GCTCTTCATG 
CTGATGTCCT ACTGCTGCTT CCTCTGCGAA TCGAATGCCA AGTCCGGCGT GGTCTTCTTC 
ATCGCCGTGA TGCTCATCTG CTTGGCCAGC ATTTGCCTCA CCATTTTCGG GGTGTTTTCC 
CTGGCCGTGG GCGGTAATGC CGAGGTCTTT GTCTGCCGAT CACTCTACGA CGAGGACTAC 
AACATGCTGG GTAAGCTGCT GGACAAGCCG GGTTACGCAT ACGCCCGGGA GCCACAGACC 
GGCATCATCG GAGAGCTACT GCGTCCTGTC GGCGTCAATC GGTCGGTGGT CAACGTTGGT 
CTGGGCCAGG CCCTCAGGGG CTGCGAGCAA AATCAAGCAT CGTACAATGT CTTTCAATTG 
GATGCGTTCG TGAACACCAG CAAAATCGCC GACCTGAGGC AGTTCCCCAA AGTGTCCACC 
GCCATCGATG CCATCTCCGT GTCCGGCCGC ACGCTCCTGT CGCTCACGCA ATCCGTGCAG 
AACATTTTGG ATAACATGCT GCAGACGTCC TCGTTCAATT TGACCACGTA CCGCAGCAGC 
GTTGGTGCGC CCACGCCCGA AAAGGATTTG GCCACGTTCA TTGATCAGAT GCAGCGGGTG 
GCCTTGCAGA TTCAGGACGT GGCTACCTCC TCGCGTATGA CCACTCTGGG CAGCCGATCC 
AAGCGGCTGC AGGCCTCGAT TCTCCAGCCG CTGGAGAACC TGCAGAACGA GATTCTCTAC 
CACCTGACGG CCCTGGAGCT ACAGCGCGAT CCCTGGGCCA AGCAGGTCAA CCAGACTCTG 
AACCACCTCA AGAGCATCCA GAGCTACCTA GAGAACAAGG CCGGGGAGAT ATGCCACAAC 
ATGACACGGG ACTACAGGGA TCGCTTGAAG GCCTACTTGA CCACCAGTAA GGCAACCATG 
GAGTCCCAGC TGGGCGACAC TACGACCAAG TGCCGACCCT TCTACGACAC CTTCGACGCC 
AGCCGCACCT TCCTCTGCCG CAACATGGTG GATTTCATCA ACGGCCTCAC CTTCTTCATC 
TTCCTTACAC TGCTTCTCTG GGCTATTGGC ACTCCGGTGG GCCTCAACCT GATCACGGTT 
CAAACGCGGC TGAATGTGAT GCAGGCGGCT CAGGCGCGGA GCAAGGGCAG GCACAGGAGG 
AGCGACCGGA GTCCGGAGCA TGAGCAGAGG AGCGGAAGTC GCGGCCGGAA GAAGCAACGG 
ACGAGCAGTT CAGCGACAAG CGGGCGGAGC GGAAAGAGGG ACCAGAGCCG GGAGAGGAGC 
ACCAGCAGCA CGGCGAGACC GAAACCACCT TTAAGACGGA CTGCCACTGA AGAAACACTT 
GATCTGGCAG ACGAAGAATC CACCCCGTCA AGACAGCAGC AGCAGCAGCC GCCGCCACAG 
CGACGCCAGG AATCGGACAG GGAGCGGGTG CGGGAGCGGG ATCAGCGAAG TCGGGAGCGG 
GAATTGAGCA GGGGCCGTGA CGCGTCCGCC ATGAACACGC CAGTGCGCAG CCGCAGTCGC 
CAGCAACTGC GCCATGACCC CGAGGATGAC AATTGGGGCT CGTCCAGCGG ACCCAGTAGG 
GCCAACTCTC GAGAGCGAGA TCGCGAGAGC AGTCAGACTA GAAAGATACT CAATATTTGG 
CAATCGCGCA ATAAGGCCAA GACGCCCCTT CGCCGTCAAG GCACCGAAGA GTTCGACTTT 
GAGGATGCCC ACGACGACAG AGGCTGGCAA GCCCAGACCC AAAACCAGGT TCAGAACCAA 
AGCCGCGCTC ATAACCAGTC CAGTGTCAGC CCCGAACAAA GGCCCGATCC GCGCAGCCAT 
CGCTCCAATC TCCGTACTAA GCCCAAGTTA CGTAGTACCA ATGCGGATAG AGCTAGCAGT 
GAAGCGATCC GGCGACCCAT AACTCCCGTG CTCAGCGTAA ATTCGGACAT TCCGGCCGCC 
ATTCCGATGC CCCGGACGCC GATGCGTCTC AAGAGCAAGG TGTCGTTGAC CGCCCGAGCT 
GTGCAGGACA TTGTCAACAA GCGGAATCAG CGCCTCCAGC GAACCGGCAC CGATGAGTTC 
GACTTGGACG ACAGCGATGC ATACGGTGCT GGAGGCCATT TGGATGCGGA CGTGGCGGCC 
GCGCGACGCA CTCCGCCCAG GGGCGCACCT CCACCCCCTC CTCCGCCAGC GGCCGGCTTG 
AACAGTGATC GCACGGTGCG CTGGAACGAG GACGAAACCG AGGACTTTGA CTTCGAGGAG 
GTCGACGCGC CGAAGAGTCC CATGTATCTG AGCCACAATC TCCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prom ( NM_001299914.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286843.1 Drosophila melanogaster prominin (prom), transcript variant E, mRNA.

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CloneID	OFa22295
Clone ID Related Accession (Same CDS sequence)	NM_001299914.1
Accession Version	NM_001299914.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	prominin, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGACCAGC GCAATCTAAC ACTGCCAACG CCAGCCGCAA CAACAATTAT GTCCTCGTCA 
TCAGCAGCGA TGTCGGCGGG CTTCGCATCC CTTGGAGGAC CCACCACGTC CGCCCTGACC 
GGATCCACAA TAGCATTAGC CACAACAACA ACAACGGCGC CGCCAACAAC GACGCCAGCC 
ACATTTTTTA CCTACAGCAT ACCAGGCATG GACATTGCGC CAGGACCATT TATATTCACC 
TACGTTAGTG AATTTTTATC GCTTATCACG CCCGAGGAAT TGCCGCTGGA TTCCATACGC 
GATGTTCTAC ACAAAAATAC CAGTCTGCTG GACTTTGCCT CCAATGCCAT TAAAATCGAG 
TCAGGCTTCA TTGCTCTGAT GAGCGTGTTC GCCATCCTGG CCCTGGTTCC CCTGATTGCC 
ACGTGTGCCT GGTGCTGCGG TCGTCGCTCC ACCCAGGAGG AGGTCGACCA CATACGGAAT 
GCACCCTGTC ACATCCGGGA CGAGTCGCTG GAGGAGAGCT TACGATGTCG CAAGAGCGCC 
GGACTGGTCA CCCTGTGGGT CGTCTTCATC CTGCTGACGC TGAGCGACTT CAGCATTTTT 
TACGCCAACA GTCGCTTGTC CGCCGGAATC GAGATGACGC CCGAGATGGT CAAGTCAGCC 
GTGCACGACG TGGAGGTCTT CCTGAAGGAC ACGCATCTAC AAATCAAGCA AAAACTGGAT 
AATGGCTTCC ACGTTTCCGT GGAGCGGGTC GTCAAAGACC TGGAAGACGT GGATGTTCTC 
CTTGGCGAGC CGATTCAAGC GGAAATATCC GCACACACTG GCCTTGAGCT GGCGTATGAT 
TCACTGACGA CACTTAGCCT GGCGAACGCG GAGCTCATCT ACCGCGTGCT CATGCTGCAG 
GAGACGGTGG GCAGGGCCCT GAAGCTCTCC CAGGAGGCGG CCGCCAGGAT GGAGGAGCTG 
CAGATCCAGC TGTCCGTGCT GCAGCGCCAG TGCACGTACC GCGACCGCCC GCTCTGCGAC 
ACGTTGCGTA TACGCAGCTT CGAGGAGAAC GGCGTCGTCG ACGCCCTGAA AACTCTGCAA 
GAGGACCAGA GCATCTTCCG TATGCGCTAC TTGGGCGAAA TTGAATTCGG TGCCACTGTC 
AAAAATCTGA CATCGGAAGT TGGCGTGTCA CGTTCCATTT TCAGCAATTT CCCGCAACAA 
GTTAAACATG ACACGGCTTA TGAGCGGAAC TATACATTGG AGCAGTTGGC CAGCATTCGC 
CACCGGACGA CTGCTAATGC CCAGCAACTG ACCTCTACGG TAAATGGACT GCTCCGGCGG 
CTGGAGGACA CCTGGTCATC CCTGCAGCCC GCCTATAACG AGCTGAAGGA GTGGGCAGAC 
GCCTACTGGA GTGTGGGCTG GATTGCCGGC ACGGTGATAG TGTGGGTAAT GCTCTTCATG 
CTGATGTCCT ACTGCTGCTT CCTCTGCGAA TCGAATGCCA AGTCCGGCGT GGTCTTCTTC 
ATCGCCGTGA TGCTCATCTG CTTGGCCAGC ATTTGCCTCA CCATTTTCGG GGTGTTTTCC 
CTGGCCGTGG GCGGTAATGC CGAGGTCTTT GTCTGCCGAT CACTCTACGA CGAGGACTAC 
AACATGCTGG GTAAGCTGCT GGACAAGCCG GGTTACGCAT ACGCCCGGGA GCCACAGACC 
GGCATCATCG GAGAGCTACT GCGTCCTGTC GGCGTCAATC GGTCGGTGGT CAACGTTGGT 
CTGGGCCAGG CCCTCAGGGG CTGCGAGCAA AATCAAGCAT CGTACAATGT CTTTCAATTG 
GATGCGTTCG TGAACACCAG CAAAATCGCC GACCTGAGGC AGTTCCCCAA AGTGTCCACC 
GCCATCGATG CCATCTCCGT GTCCGGCCGC ACGCTCCTGT CGCTCACGCA ATCCGTGCAG 
AACATTTTGG ATAACATGCT GCAGACGTCC TCGTTCAATT TGACCACGTA CCGCAGCAGC 
GTTGGTGCGC CCACGCCCGA AAAGGATTTG GCCACGTTCA TTGATCAGAT GCAGCGGGTG 
GCCTTGCAGA TTCAGGACGT GGCTACCTCC TCGCGTATGA CCACTCTGGG CAGCCGATCC 
AAGCGGCTGC AGGCCTCGAT TCTCCAGCCG CTGGAGAACC TGCAGAACGA GATTCTCTAC 
CACCTGACGG CCCTGGAGCT ACAGCGCGAT CCCTGGGCCA AGCAGGTCAA CCAGACTCTG 
AACCACCTCA AGAGCATCCA GAGCTACCTA GAGAACAAGG CCGGGGAGAT ATGCCACAAC 
ATGACACGGG ACTACAGGGA TCGCTTGAAG GCCTACTTGA CCACCAGTAA GGCAACCATG 
GAGTCCCAGC TGGGCGACAC TACGACCAAG TGCCGACCCT TCTACGACAC CTTCGACGCC 
AGCCGCACCT TCCTCTGCCG CAACATGGTG GATTTCATCA ACGGCCTCAC CTTCTTCATC 
TTCCTTACAC TGCTTCTCTG GGCTATTGGC ACTCCGGTGG GCCTCAACCT GATCACGGTT 
CAAACGCGGC TGAATGTGAT GCAGGCGGCT CAGGCGCGGA GCAAGGGCAG GCACAGGAGG 
AGCGACCGGA GTCCGGAGCA TGAGCAGAGG AGCGGAAGTC GCGGCCGGAA GAAGCAACGG 
ACGAGCAGTT CAGCGACAAG CGGGCGGAGC GGAAAGAGGG ACCAGAGCCG GGAGAGGAGC 
ACCAGCAGCA CGGCGAGACC GAAACCACCT TTAAGACGGA CTGCCACTGA AGAAACACTT 
GATCTGGCAG ACGAAGAATC CACCCCGTCA AGACAGCAGC AGCAGCAGCC GCCGCCACAG 
CGACGCCAGG AATCGGACAG GGAGCGGGTG CGGGAGCGGG ATCAGCGAAG TCGGGAGCGG 
GAATTGAGCA GGGGCCGTGA CGCGTCCGCC ATGAACACGC CAGTGCGCAG CCGCAGTCGC 
CAGCAACTGC GCCATGACCC CGAGGATGAC AATTGGGGCT CGTCCAGCGG ACCCAGTAGG 
GCCAACTCTC GAGAGCGAGA TCGCGAGAGC AGTCAGACTA GAAAGATACT CAATATTTGG 
CAATCGCGCA ATAAGGCCAA GACGCCCCTT CGCCGTCAAG GCACCGAAGA GTTCGACTTT 
GAGGATGCCC ACGACGACAG AGGCTGGCAA GCCCAGACCC AAAACCAGGT TCAGAACCAA 
AGCCGCGCTC ATAACCAGTC CAGTGTCAGC CCCGAACAAA GGCCCGATCC GCGCAGCCAT 
CGCTCCAATC TCCGTACTAA GCCCAAGTTC CAGCGAACCG GCACCGATGA GTTCGACTTG 
GACGACAGCG ATGCATACGG TGCTGGAGGC CATTTGGATG CGGACGTGGC GGCCGCGCGA 
CGCACTCCGC CCAGGGGCGC ACCTCCACCC CCTCCTCCGC CAGCGGCCGG CTTGAACAGT 
GATCGCACGG TGCGCTGGAA CGAGGACGAA ACCGAGGACT TTGACTTCGA GGAGGTCGAC 
GCGCCGAAGA GTCCCATGTA TCTGAGCCAC AATCTCCAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286843.1
CDS	507..4028
Translation	MDQRNLTLPTPAATTIMSSSSAAMSAGFASLGGPTTSALTGSTIALATTTTTAPPTTTPATFFTYSIPGMDIAPGPFIFTYVSEFLSLITPEELPLDSIRDVLHKNTSLLDFASNAIKIESGFIALMSVFAILALVPLIATCAWCCGRRSTQEEVDHIRNAPCHIRDESLEESLRCRKSAGLVTLWVVFILLTLSDFSIFYANSRLSAGIEMTPEMVKSAVHDVEVFLKDTHLQIKQKLDNGFHVSVERVVKDLEDVDVLLGEPIQAEISAHTGLELAYDSLTTLSLANAELIYRVLMLQETVGRALKLSQEAAARMEELQIQLSVLQRQCTYRDRPLCDTLRIRSFEENGVVDALKTLQEDQSIFRMRYLGEIEFGATVKNLTSEVGVSRSIFSNFPQQVKHDTAYERNYTLEQLASIRHRTTANAQQLTSTVNGLLRRLEDTWSSLQPAYNELKEWADAYWSVGWIAGTVIVWVMLFMLMSYCCFLCESNAKSGVVFFIAVMLICLASICLTIFGVFSLAVGGNAEVFVCRSLYDEDYNMLGKLLDKPGYAYAREPQTGIIGELLRPVGVNRSVVNVGLGQALRGCEQNQASYNVFQLDAFVNTSKIADLRQFPKVSTAIDAISVSGRTLLSLTQSVQNILDNMLQTSSFNLTTYRSSVGAPTPEKDLATFIDQMQRVALQIQDVATSSRMTTLGSRSKRLQASILQPLENLQNEILYHLTALELQRDPWAKQVNQTLNHLKSIQSYLENKAGEICHNMTRDYRDRLKAYLTTSKATMESQLGDTTTKCRPFYDTFDASRTFLCRNMVDFINGLTFFIFLTLLLWAIGTPVGLNLITVQTRLNVMQAAQARSKGRHRRSDRSPEHEQRSGSRGRKKQRTSSSATSGRSGKRDQSRERSTSSTARPKPPLRRTATEETLDLADEESTPSRQQQQQPPPQRRQESDRERVRERDQRSRERELSRGRDASAMNTPVRSRSRQQLRHDPEDDNWGSSSGPSRANSRERDRESSQTRKILNIWQSRNKAKTPLRRQGTEEFDFEDAHDDRGWQAQTQNQVQNQSRAHNQSSVSPEQRPDPRSHRSNLRTKPKFQRTGTDEFDLDDSDAYGAGGHLDADVAAARRTPPRGAPPPPPPPAAGLNSDRTVRWNEDETEDFDFEEVDAPKSPMYLSHNLQ

Target ORF information:

RefSeq Version	NM_001299914.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster prominin (prom), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299914.1

ORF Insert Sequence:

ATGGACCAGC GCAATCTAAC ACTGCCAACG CCAGCCGCAA CAACAATTAT GTCCTCGTCA 
TCAGCAGCGA TGTCGGCGGG CTTCGCATCC CTTGGAGGAC CCACCACGTC CGCCCTGACC 
GGATCCACAA TAGCATTAGC CACAACAACA ACAACGGCGC CGCCAACAAC GACGCCAGCC 
ACATTTTTTA CCTACAGCAT ACCAGGCATG GACATTGCGC CAGGACCATT TATATTCACC 
TACGTTAGTG AATTTTTATC GCTTATCACG CCCGAGGAAT TGCCGCTGGA TTCCATACGC 
GATGTTCTAC ACAAAAATAC CAGTCTGCTG GACTTTGCCT CCAATGCCAT TAAAATCGAG 
TCAGGCTTCA TTGCTCTGAT GAGCGTGTTC GCCATCCTGG CCCTGGTTCC CCTGATTGCC 
ACGTGTGCCT GGTGCTGCGG TCGTCGCTCC ACCCAGGAGG AGGTCGACCA CATACGGAAT 
GCACCCTGTC ACATCCGGGA CGAGTCGCTG GAGGAGAGCT TACGATGTCG CAAGAGCGCC 
GGACTGGTCA CCCTGTGGGT CGTCTTCATC CTGCTGACGC TGAGCGACTT CAGCATTTTT 
TACGCCAACA GTCGCTTGTC CGCCGGAATC GAGATGACGC CCGAGATGGT CAAGTCAGCC 
GTGCACGACG TGGAGGTCTT CCTGAAGGAC ACGCATCTAC AAATCAAGCA AAAACTGGAT 
AATGGCTTCC ACGTTTCCGT GGAGCGGGTC GTCAAAGACC TGGAAGACGT GGATGTTCTC 
CTTGGCGAGC CGATTCAAGC GGAAATATCC GCACACACTG GCCTTGAGCT GGCGTATGAT 
TCACTGACGA CACTTAGCCT GGCGAACGCG GAGCTCATCT ACCGCGTGCT CATGCTGCAG 
GAGACGGTGG GCAGGGCCCT GAAGCTCTCC CAGGAGGCGG CCGCCAGGAT GGAGGAGCTG 
CAGATCCAGC TGTCCGTGCT GCAGCGCCAG TGCACGTACC GCGACCGCCC GCTCTGCGAC 
ACGTTGCGTA TACGCAGCTT CGAGGAGAAC GGCGTCGTCG ACGCCCTGAA AACTCTGCAA 
GAGGACCAGA GCATCTTCCG TATGCGCTAC TTGGGCGAAA TTGAATTCGG TGCCACTGTC 
AAAAATCTGA CATCGGAAGT TGGCGTGTCA CGTTCCATTT TCAGCAATTT CCCGCAACAA 
GTTAAACATG ACACGGCTTA TGAGCGGAAC TATACATTGG AGCAGTTGGC CAGCATTCGC 
CACCGGACGA CTGCTAATGC CCAGCAACTG ACCTCTACGG TAAATGGACT GCTCCGGCGG 
CTGGAGGACA CCTGGTCATC CCTGCAGCCC GCCTATAACG AGCTGAAGGA GTGGGCAGAC 
GCCTACTGGA GTGTGGGCTG GATTGCCGGC ACGGTGATAG TGTGGGTAAT GCTCTTCATG 
CTGATGTCCT ACTGCTGCTT CCTCTGCGAA TCGAATGCCA AGTCCGGCGT GGTCTTCTTC 
ATCGCCGTGA TGCTCATCTG CTTGGCCAGC ATTTGCCTCA CCATTTTCGG GGTGTTTTCC 
CTGGCCGTGG GCGGTAATGC CGAGGTCTTT GTCTGCCGAT CACTCTACGA CGAGGACTAC 
AACATGCTGG GTAAGCTGCT GGACAAGCCG GGTTACGCAT ACGCCCGGGA GCCACAGACC 
GGCATCATCG GAGAGCTACT GCGTCCTGTC GGCGTCAATC GGTCGGTGGT CAACGTTGGT 
CTGGGCCAGG CCCTCAGGGG CTGCGAGCAA AATCAAGCAT CGTACAATGT CTTTCAATTG 
GATGCGTTCG TGAACACCAG CAAAATCGCC GACCTGAGGC AGTTCCCCAA AGTGTCCACC 
GCCATCGATG CCATCTCCGT GTCCGGCCGC ACGCTCCTGT CGCTCACGCA ATCCGTGCAG 
AACATTTTGG ATAACATGCT GCAGACGTCC TCGTTCAATT TGACCACGTA CCGCAGCAGC 
GTTGGTGCGC CCACGCCCGA AAAGGATTTG GCCACGTTCA TTGATCAGAT GCAGCGGGTG 
GCCTTGCAGA TTCAGGACGT GGCTACCTCC TCGCGTATGA CCACTCTGGG CAGCCGATCC 
AAGCGGCTGC AGGCCTCGAT TCTCCAGCCG CTGGAGAACC TGCAGAACGA GATTCTCTAC 
CACCTGACGG CCCTGGAGCT ACAGCGCGAT CCCTGGGCCA AGCAGGTCAA CCAGACTCTG 
AACCACCTCA AGAGCATCCA GAGCTACCTA GAGAACAAGG CCGGGGAGAT ATGCCACAAC 
ATGACACGGG ACTACAGGGA TCGCTTGAAG GCCTACTTGA CCACCAGTAA GGCAACCATG 
GAGTCCCAGC TGGGCGACAC TACGACCAAG TGCCGACCCT TCTACGACAC CTTCGACGCC 
AGCCGCACCT TCCTCTGCCG CAACATGGTG GATTTCATCA ACGGCCTCAC CTTCTTCATC 
TTCCTTACAC TGCTTCTCTG GGCTATTGGC ACTCCGGTGG GCCTCAACCT GATCACGGTT 
CAAACGCGGC TGAATGTGAT GCAGGCGGCT CAGGCGCGGA GCAAGGGCAG GCACAGGAGG 
AGCGACCGGA GTCCGGAGCA TGAGCAGAGG AGCGGAAGTC GCGGCCGGAA GAAGCAACGG 
ACGAGCAGTT CAGCGACAAG CGGGCGGAGC GGAAAGAGGG ACCAGAGCCG GGAGAGGAGC 
ACCAGCAGCA CGGCGAGACC GAAACCACCT TTAAGACGGA CTGCCACTGA AGAAACACTT 
GATCTGGCAG ACGAAGAATC CACCCCGTCA AGACAGCAGC AGCAGCAGCC GCCGCCACAG 
CGACGCCAGG AATCGGACAG GGAGCGGGTG CGGGAGCGGG ATCAGCGAAG TCGGGAGCGG 
GAATTGAGCA GGGGCCGTGA CGCGTCCGCC ATGAACACGC CAGTGCGCAG CCGCAGTCGC 
CAGCAACTGC GCCATGACCC CGAGGATGAC AATTGGGGCT CGTCCAGCGG ACCCAGTAGG 
GCCAACTCTC GAGAGCGAGA TCGCGAGAGC AGTCAGACTA GAAAGATACT CAATATTTGG 
CAATCGCGCA ATAAGGCCAA GACGCCCCTT CGCCGTCAAG GCACCGAAGA GTTCGACTTT 
GAGGATGCCC ACGACGACAG AGGCTGGCAA GCCCAGACCC AAAACCAGGT TCAGAACCAA 
AGCCGCGCTC ATAACCAGTC CAGTGTCAGC CCCGAACAAA GGCCCGATCC GCGCAGCCAT 
CGCTCCAATC TCCGTACTAA GCCCAAGTTC CAGCGAACCG GCACCGATGA GTTCGACTTG 
GACGACAGCG ATGCATACGG TGCTGGAGGC CATTTGGATG CGGACGTGGC GGCCGCGCGA 
CGCACTCCGC CCAGGGGCGC ACCTCCACCC CCTCCTCCGC CAGCGGCCGG CTTGAACAGT 
GATCGCACGG TGCGCTGGAA CGAGGACGAA ACCGAGGACT TTGACTTCGA GGAGGTCGAC 
GCGCCGAAGA GTCCCATGTA TCTGAGCCAC AATCTCCAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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prom cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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