LOC109546665 cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC109546665
Entrez Gene ID	109546665
Full Name	mediator of DNA damage checkpoint protein 1-like
Gene Type	protein-coding
Organism	Dendroctonus ponderosae(mountain pine beetle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019917715.1	XP_019773274.1	mediator of DNA damage checkpoint protein 1-like isoform X2
XM_019917713.1	XP_019773272.1	mediator of DNA damage checkpoint protein 1-like isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC109546665 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC109546665 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODe35667	XM_019917713.1 Latest version!	Dendroctonus ponderosae mediator of DNA damage checkpoint protein 1-like (LOC109546665), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODe35668	XM_019917715.1 Latest version!	Dendroctonus ponderosae mediator of DNA damage checkpoint protein 1-like (LOC109546665), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC109546665 ( XM_019917713.1 ) cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle) -> XP_019773272.1 Dendroctonus ponderosae mediator of DNA damage checkpoint protein 1-like (LOC109546665), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODe35667
Clone ID Related Accession (Same CDS sequence)	XM_019917713.1
Accession Version	XM_019917713.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-09
Organism	Dendroctonus ponderosae(mountain pine beetle)
Product	mediator of DNA damage checkpoint protein 1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017851173.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Dendroctonus ponderosae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCGC CGGGCATTGG CCCGCAGATG GGCCTTTTTA CAATAAACAA TCAGAAATTT 
CCTCTTTACG AGGGTCTAAA CACAATAGGA CGCAACAGAG AGGCCGTGGT GAATATCAAG 
CATGTTCTCA TTAGTCAACA TCATGCGCTT ATTACGGTAC TGGACGGGAA GTTTTTCATC 
AGCGACCTGA AATCCAGCAA CGGCAGTTTC CTGGAAGGTA TGCGACTGGT TCCCTTGAAA 
ATGTATCCTT TCAATAACGA CGCCAAAATC ACCCTGGGGA ACTTGCACGG AACCTTCACT 
ATCACATCGG ATCCCCCCCT TGAGGAGCGA TTGATTGAGA CTCAAACTCA GTCATTATTT 
GATGGAGGCA CCCAACTGAT GCAGTCCACT GGGAATGTTC AGGAAGAACT CACTCAGAAG 
ATTTTGTGGC CCCCGTCGGT TCCCACGTAT GTACCAGGGC TTCAAATCGG CGAAATCCAT 
GATGAGCCTT TGTCGGTTTT GGAAAATTAT GTTGAACTCG AACCAGATCC ACAGTTGGAA 
AGGGTTGAAG ATCTGCCTTC TTCGCCTGTG GTGATCAGCG ACGATGAAGT CACGGACTGT 
GAGGAAAACC CTGGTCCTAG TGGAGAGCAT GATGCAAATA ACCATGAGGC CACGTTTTGT 
GACAAAAGTA ATGAATCTCT GGAGTTTTTG AACCAGTCCC CAACCACGGC ACAGCCTAAT 
GGAGAAGAAA GCCGCGATTC CAGTGATGAT GATTCTTTTG CGTTCAAGCG AACCCGGAAG 
TCTTTTATGG TGATTTCCAC TCAGGATCTT CAAGAAAACG CGAGTGAGTC CAGTAGCCAT 
CAGTTGGAGG AAACGATTCC AGCCACCCAA TATCCATCAG CCCTGGAGTT GAATGAAAGC 
GACATTATTG CGGCCACTCA AGCTTTGCGC CCGGATAGTC AAAGCAGCGA GTCGGAGTTT 
TTCAAATTGG GGCTAACGCA GTTGATGGAT TCTAGCCTGA ACGGGATCAC AAGTCAGGCC 
GCAGAACCAG CACCAGAAGA AAACGAATCA ATCGACACAA CCCACGTGAG CCTCGCTCAG 
GCCGTGCAGA ACAACGAAGA CATCTATGAG GTAGCCACTC AAAAAGTTGC GTCCACTGCC 
GACGAGACGT TCAATATCTT TGACGCTCCC ACTCAAAAAC TCCGCACAGT GGAGGATTCA 
TTTCTTCAAC CCACACAACC GATGTTCTCC GAAGCGCTAG AAGACGTTGA CTATGACAGC 
CCCACTCAGA AAGTCTCTGA CGATGATGAT ACTTTTCTGG CACCCACTCA GAAGCTGAGC 
TTCAGCGCGG CTGCACCCAA AGAGAATGAG GACTTTTTCT TGGCTCCAAC GCAGAGGGTT 
CTGCGTCCGA AACGGGTGTA TTCCTTCAAG AAAACCAAGT GCAATCTAGA TGCTTCGCTT 
TCGGTTTTGG CCGTAGGTGA AGCTGAAACT GGCCAAGCGA AACTCGATTC TGCAGCAACG 
GGGAAGAAAA CCGACGACGA AGTCATTGCT CAACTAGATA AAGAAACATT CATGGATAGT 
CCAACTAGGG ATATTTCCAA GCCTCAAGAT GCCGAAGATG TTGAGGGTTT GCAGGCGAAA 
CAGGACACAG CCACGGGGGC CAAAGGCGAG GATTATTTCA AAACAAGTAA TGCGGTTAAA 
CTGCAAGCGA AGAAAAGTGA AAGCCATGTA AAAATACGCG AGTCTTTGAA TGATGAAGAA 
ATCGAAGCCA AGCCTAAACA GGCCTCTGGA AACCGTGAAA CTTTTGCGGC TGGTGAAAAT 
ACATGCAGCA AGCCCCAGCT GCCGAACGGT TCCCACTCGT CTGTAGAGGC CAAATCTGAA 
GAAACGATCG ATAGAACATC TATGCCCTCT AAACGCGAAC GAGCTGCAAA TTCTATTAAC 
CCTTTTATCA TCGAAGACGC TAAAGAAAGA TCTGACAAAA CCTTTAAATG CCCTGAAAGT 
AGTTATGTAG CTTCTGATGC TAATCAGGAA ACGGTTCGAA GTGACTGCAA AAACTCCCAA 
ATAAATGGCG AAATTCCCGT TGAGGCCAAT AATGATGAAC CAGCCAAGTC GGCACACAAT 
TTTGCTAGCG AAGATATTGA AAAACCCCCC AAAAGGCTGA AGAGACAGTG CCGAATTAAA 
CCATCTGTGG AGGCTAAAAG TGGGGATAAA CCTAAGAAAC CGGCTAATCT CGAGGAAGAT 
GTAGCAGTAG TCTGTGTAGA GGAAACAAGC AAAGAAGAAA TAGCAAACCC GACTCACATC 
GACCAATCGA TGGCATTGCT GGCTTCAAAA ATGATCAAAA CGAGGAAGAC GACCAAAACT 
GAGGAAATCC GAATCAACTT TCCGAAAGAG AGGAAAAGCA TGGCGTTAAG TGTGGATGAA 
GAGGAGATGC CAAGGAGCCG GCGAGCGGCA GCAGAAGGAA AGCGCGCCAA GACCTCAAAG 
CCGAATGAAA AACCACCAGC AACAAAAGCG GCCAAACGAG CCAATAAAGA ACGGGCCGCA 
GATGAAGGGA GCTTATCCAA GAAACAGAGG CTTCAATCAG AAGACTCACC GAGAGGAAAC 
CTGCGGAAAC AACGCCACAA AGTGGTCTTC ACCATGCTGA ACAGCCCCCA ATTGGAATCT 
CTGATCAAGC AACTAGGCGG CAGCATTGTG GACTCGGTGG ACTCTTGCAC CGTTCTGATC 
ACTGAGCACG TGAAAAGGTC TCAGAAGCTC CTAGAAGCCA TTGGCAGGGG ACGACCGATC 
TGCTCACCTC AATGGGTATT GGACTCGAAA CGCGCCAAGC AGTTCCTAGA TCCTTGGGAC 
TATATCCTGA CCGATGCGGA AGCGGAGACA AATTGGGAAT TTTCTCTGAA CGAGTCGTTG 
CAACGGAGCA TGGCGCAGAA CCTGCTGAAG GACTACAACT TCTATATCCA AGTGACCAAT 
GGAGTGGACG TCCTTAAAGG AGGAATTAAG GCCTGCGGCG GCAAATGTCT GGCGCGGATC 
CCATCCGGGC CCCTTCAGCA CGCCTATGTG ATTTCGACGG TCGAGAATCG CACAAAACAC 
AACAAAATCC TGCAGAAACA TCCGCATTTC CAGGCCATAG AAGCCGAAGC TGTGTTTGAT 
GCGATTTTGA GGCAGGAAAT CCGATTTACT CGGCATATTA TAATTCTCGA TCACTCCGCA 
ATCGTACATC AACCCACTTG CTTCTGGATA TGGAAACACC CTGTAGATGA TGTTAAATGG 
CCAATAAGCA CCGGGAAATT AATGAATATT AATATGACAA TGGATCCGGC CTATAAGAAA 
TCTGCAAAGG TCGCCCCAGT TAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019773272.1
CDS	134..3460
Translation	MQPPGIGPQMGLFTINNQKFPLYEGLNTIGRNREAVVNIKHVLISQHHALITVLDGKFFISDLKSSNGSFLEGMRLVPLKMYPFNNDAKITLGNLHGTFTITSDPPLEERLIETQTQSLFDGGTQLMQSTGNVQEELTQKILWPPSVPTYVPGLQIGEIHDEPLSVLENYVELEPDPQLERVEDLPSSPVVISDDEVTDCEENPGPSGEHDANNHEATFCDKSNESLEFLNQSPTTAQPNGEESRDSSDDDSFAFKRTRKSFMVISTQDLQENASESSSHQLEETIPATQYPSALELNESDIIAATQALRPDSQSSESEFFKLGLTQLMDSSLNGITSQAAEPAPEENESIDTTHVSLAQAVQNNEDIYEVATQKVASTADETFNIFDAPTQKLRTVEDSFLQPTQPMFSEALEDVDYDSPTQKVSDDDDTFLAPTQKLSFSAAAPKENEDFFLAPTQRVLRPKRVYSFKKTKCNLDASLSVLAVGEAETGQAKLDSAATGKKTDDEVIAQLDKETFMDSPTRDISKPQDAEDVEGLQAKQDTATGAKGEDYFKTSNAVKLQAKKSESHVKIRESLNDEEIEAKPKQASGNRETFAAGENTCSKPQLPNGSHSSVEAKSEETIDRTSMPSKRERAANSINPFIIEDAKERSDKTFKCPESSYVASDANQETVRSDCKNSQINGEIPVEANNDEPAKSAHNFASEDIEKPPKRLKRQCRIKPSVEAKSGDKPKKPANLEEDVAVVCVEETSKEEIANPTHIDQSMALLASKMIKTRKTTKTEEIRINFPKERKSMALSVDEEEMPRSRRAAAEGKRAKTSKPNEKPPATKAAKRANKERAADEGSLSKKQRLQSEDSPRGNLRKQRHKVVFTMLNSPQLESLIKQLGGSIVDSVDSCTVLITEHVKRSQKLLEAIGRGRPICSPQWVLDSKRAKQFLDPWDYILTDAEAETNWEFSLNESLQRSMAQNLLKDYNFYIQVTNGVDVLKGGIKACGGKCLARIPSGPLQHAYVISTVENRTKHNKILQKHPHFQAIEAEAVFDAILRQEIRFTRHIIILDHSAIVHQPTCFWIWKHPVDDVKWPISTGKLMNINMTMDPAYKKSAKVAPVN

Target ORF information:

RefSeq Version	XM_019917713.1
Organism	Dendroctonus ponderosae(mountain pine beetle)
Definition	Dendroctonus ponderosae mediator of DNA damage checkpoint protein 1-like (LOC109546665), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019917713.1

ORF Insert Sequence:

ATGCAGCCGC CGGGCATTGG CCCGCAGATG GGCCTTTTTA CAATAAACAA TCAGAAATTT 
CCTCTTTACG AGGGTCTAAA CACAATAGGA CGCAACAGAG AGGCCGTGGT GAATATCAAG 
CATGTTCTCA TTAGTCAACA TCATGCGCTT ATTACGGTAC TGGACGGGAA GTTTTTCATC 
AGCGACCTGA AATCCAGCAA CGGCAGTTTC CTGGAAGGTA TGCGACTGGT TCCCTTGAAA 
ATGTATCCTT TCAATAACGA CGCCAAAATC ACCCTGGGGA ACTTGCACGG AACCTTCACT 
ATCACATCGG ATCCCCCCCT TGAGGAGCGA TTGATTGAGA CTCAAACTCA GTCATTATTT 
GATGGAGGCA CCCAACTGAT GCAGTCCACT GGGAATGTTC AGGAAGAACT CACTCAGAAG 
ATTTTGTGGC CCCCGTCGGT TCCCACGTAT GTACCAGGGC TTCAAATCGG CGAAATCCAT 
GATGAGCCTT TGTCGGTTTT GGAAAATTAT GTTGAACTCG AACCAGATCC ACAGTTGGAA 
AGGGTTGAAG ATCTGCCTTC TTCGCCTGTG GTGATCAGCG ACGATGAAGT CACGGACTGT 
GAGGAAAACC CTGGTCCTAG TGGAGAGCAT GATGCAAATA ACCATGAGGC CACGTTTTGT 
GACAAAAGTA ATGAATCTCT GGAGTTTTTG AACCAGTCCC CAACCACGGC ACAGCCTAAT 
GGAGAAGAAA GCCGCGATTC CAGTGATGAT GATTCTTTTG CGTTCAAGCG AACCCGGAAG 
TCTTTTATGG TGATTTCCAC TCAGGATCTT CAAGAAAACG CGAGTGAGTC CAGTAGCCAT 
CAGTTGGAGG AAACGATTCC AGCCACCCAA TATCCATCAG CCCTGGAGTT GAATGAAAGC 
GACATTATTG CGGCCACTCA AGCTTTGCGC CCGGATAGTC AAAGCAGCGA GTCGGAGTTT 
TTCAAATTGG GGCTAACGCA GTTGATGGAT TCTAGCCTGA ACGGGATCAC AAGTCAGGCC 
GCAGAACCAG CACCAGAAGA AAACGAATCA ATCGACACAA CCCACGTGAG CCTCGCTCAG 
GCCGTGCAGA ACAACGAAGA CATCTATGAG GTAGCCACTC AAAAAGTTGC GTCCACTGCC 
GACGAGACGT TCAATATCTT TGACGCTCCC ACTCAAAAAC TCCGCACAGT GGAGGATTCA 
TTTCTTCAAC CCACACAACC GATGTTCTCC GAAGCGCTAG AAGACGTTGA CTATGACAGC 
CCCACTCAGA AAGTCTCTGA CGATGATGAT ACTTTTCTGG CACCCACTCA GAAGCTGAGC 
TTCAGCGCGG CTGCACCCAA AGAGAATGAG GACTTTTTCT TGGCTCCAAC GCAGAGGGTT 
CTGCGTCCGA AACGGGTGTA TTCCTTCAAG AAAACCAAGT GCAATCTAGA TGCTTCGCTT 
TCGGTTTTGG CCGTAGGTGA AGCTGAAACT GGCCAAGCGA AACTCGATTC TGCAGCAACG 
GGGAAGAAAA CCGACGACGA AGTCATTGCT CAACTAGATA AAGAAACATT CATGGATAGT 
CCAACTAGGG ATATTTCCAA GCCTCAAGAT GCCGAAGATG TTGAGGGTTT GCAGGCGAAA 
CAGGACACAG CCACGGGGGC CAAAGGCGAG GATTATTTCA AAACAAGTAA TGCGGTTAAA 
CTGCAAGCGA AGAAAAGTGA AAGCCATGTA AAAATACGCG AGTCTTTGAA TGATGAAGAA 
ATCGAAGCCA AGCCTAAACA GGCCTCTGGA AACCGTGAAA CTTTTGCGGC TGGTGAAAAT 
ACATGCAGCA AGCCCCAGCT GCCGAACGGT TCCCACTCGT CTGTAGAGGC CAAATCTGAA 
GAAACGATCG ATAGAACATC TATGCCCTCT AAACGCGAAC GAGCTGCAAA TTCTATTAAC 
CCTTTTATCA TCGAAGACGC TAAAGAAAGA TCTGACAAAA CCTTTAAATG CCCTGAAAGT 
AGTTATGTAG CTTCTGATGC TAATCAGGAA ACGGTTCGAA GTGACTGCAA AAACTCCCAA 
ATAAATGGCG AAATTCCCGT TGAGGCCAAT AATGATGAAC CAGCCAAGTC GGCACACAAT 
TTTGCTAGCG AAGATATTGA AAAACCCCCC AAAAGGCTGA AGAGACAGTG CCGAATTAAA 
CCATCTGTGG AGGCTAAAAG TGGGGATAAA CCTAAGAAAC CGGCTAATCT CGAGGAAGAT 
GTAGCAGTAG TCTGTGTAGA GGAAACAAGC AAAGAAGAAA TAGCAAACCC GACTCACATC 
GACCAATCGA TGGCATTGCT GGCTTCAAAA ATGATCAAAA CGAGGAAGAC GACCAAAACT 
GAGGAAATCC GAATCAACTT TCCGAAAGAG AGGAAAAGCA TGGCGTTAAG TGTGGATGAA 
GAGGAGATGC CAAGGAGCCG GCGAGCGGCA GCAGAAGGAA AGCGCGCCAA GACCTCAAAG 
CCGAATGAAA AACCACCAGC AACAAAAGCG GCCAAACGAG CCAATAAAGA ACGGGCCGCA 
GATGAAGGGA GCTTATCCAA GAAACAGAGG CTTCAATCAG AAGACTCACC GAGAGGAAAC 
CTGCGGAAAC AACGCCACAA AGTGGTCTTC ACCATGCTGA ACAGCCCCCA ATTGGAATCT 
CTGATCAAGC AACTAGGCGG CAGCATTGTG GACTCGGTGG ACTCTTGCAC CGTTCTGATC 
ACTGAGCACG TGAAAAGGTC TCAGAAGCTC CTAGAAGCCA TTGGCAGGGG ACGACCGATC 
TGCTCACCTC AATGGGTATT GGACTCGAAA CGCGCCAAGC AGTTCCTAGA TCCTTGGGAC 
TATATCCTGA CCGATGCGGA AGCGGAGACA AATTGGGAAT TTTCTCTGAA CGAGTCGTTG 
CAACGGAGCA TGGCGCAGAA CCTGCTGAAG GACTACAACT TCTATATCCA AGTGACCAAT 
GGAGTGGACG TCCTTAAAGG AGGAATTAAG GCCTGCGGCG GCAAATGTCT GGCGCGGATC 
CCATCCGGGC CCCTTCAGCA CGCCTATGTG ATTTCGACGG TCGAGAATCG CACAAAACAC 
AACAAAATCC TGCAGAAACA TCCGCATTTC CAGGCCATAG AAGCCGAAGC TGTGTTTGAT 
GCGATTTTGA GGCAGGAAAT CCGATTTACT CGGCATATTA TAATTCTCGA TCACTCCGCA 
ATCGTACATC AACCCACTTG CTTCTGGATA TGGAAACACC CTGTAGATGA TGTTAAATGG 
CCAATAAGCA CCGGGAAATT AATGAATATT AATATGACAA TGGATCCGGC CTATAAGAAA 
TCTGCAAAGG TCGCCCCAGT TAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC109546665 ( XM_019917715.1 ) cDNA ORF clone, Dendroctonus ponderosae(mountain pine beetle) -> XP_019773274.1 Dendroctonus ponderosae mediator of DNA damage checkpoint protein 1-like (LOC109546665), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODe35668
Clone ID Related Accession (Same CDS sequence)	XM_019917715.1
Accession Version	XM_019917715.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3168bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-09
Organism	Dendroctonus ponderosae(mountain pine beetle)
Product	mediator of DNA damage checkpoint protein 1-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017851173.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Dendroctonus ponderosae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCGC CGGGCATTGG CCCGCAGATG GGCCTTTTTA CAATAAACAA TCAGAAATTT 
CCTCTTTACG AGGGTCTAAA CACAATAGGA CGCAACAGAG AGGCCGTGGT GAATATCAAG 
CATGTTCTCA TTAGTCAACA TCATGCGCTT ATTACGGTAC TGGACGGGAA GTTTTTCATC 
AGCGACCTGA AATCCAGCAA CGGCAGTTTC CTGGAAGGTA TGCGACTGGT TCCCTTGAAA 
ATGTATCCTT TCAATAACGA CGCCAAAATC ACCCTGGGGA ACTTGCACGG AACCTTCACT 
ATCACATCGG ATCCCCCCCT TGAGGAGCGA TTGATTGAGA CTCAAACTCA GTCATTATTT 
GATGGAGGCA CCCAACTGAT GCAGTCCACT GGGAATGTTC AGGAAGAACT CACTCAGAAG 
ATTTTGTGGC CCCCGTCGGT TCCCACGTAT GTACCAGGGC TTCAAATCGG CGAAATCCAT 
GATGAGCCTT TGTCGGTTTT GGAAAATTAT GTTGAACTCG AACCAGATCC ACAGTTGGAA 
AGGGTTGAAG ATCTGCCTTC TTCGCCTGTG GTGATCAGCG ACGATGAAGT CACGGACTGT 
GAGGAAAACC CTGGTCCTAG TGGAGAGCAT GATGCAAATA ACCATGAGGC CACGTTTTGT 
GACAAAAGTA ATGAATCTCT GGAGTTTTTG AACCAGTCCC CAACCACGGC ACAGCCTAAT 
GGAGAAGAAA GCCGCGATTC CAGTGATGAT GATTCTTTTG CGTTCAAGCG AACCCGGAAG 
TCTTTTATGG TGATTTCCAC TCAGGATCTT CAAGAAAACG CGAGTGAGTC CAGTAGCCAT 
CAGTTGGAGG AAACGATTCC AGCCACCCAA TATCCATCAG CCCTGGAGTT GAATGAAAGC 
GACATTATTG CGGCCACTCA AGCTTTGCGC CCGGATAGTC AAAGCAGCGA GTCGGAGTTT 
TTCAAATTGG GGCTAACGCA GTTGATGGAT TCTAGCCTGA ACGGGATCAC AAGTCAGGCC 
GCAGAACCAG CACCAGAAGA AAACGAATCA ATCGACACAA CCCACGTGAG CCTCGCTCAG 
GCCGTGCAGA ACAACGAAGA CATCTATGAG GTAGCCACTC AAAAAGTTGC GTCCACTGCC 
GACGAGACGT TCAATATCTT TGACGCTCCC ACTCAAAAAC TCCGCACAGT GGAGGATTCA 
TTTCTTCAAC CCACACAACC GATGTTCTCC GAAGCGCTAG AAGACGTTGA CTATGACAGC 
CCCACTCAGA AAGTCTCTGA CGATGATGAT ACTTTTCTGG CACCCACTCA GAAGCTGAGC 
TTCAGCGCGG CTGCACCCAA AGAGAATGAG GACTTTTTCT TGGCTCCAAC GCAGAGGGTT 
CTGCGTCCGA AACGGGTGTA TTCCTTCAAG AAAACCAAGT GCAATCTAGA TGCTTCGCTT 
TCGGTTTTGG CCGTAGGTGA AGCTGAAACT GGCCAAGCGA AACTCGATTC TGCAGCAACG 
GGGAAGAAAA CCGACGACGA AGTCATTGCT CAACTAGATA AAGAAACATT CATGGATAGT 
CCAACTAGGG ATATTTCCAA GCCTCAAGAT GCCGAAGATG TTGAGGGTTT GCAGGCGAAA 
CAGGACACAG CCACGGGGGC CAAAGGCGAG GATTATTTCA AAACAAGTAA TGCGGTTAAA 
CTGCAAGCGA AGAAAAGTGA AAGCCATGTA AAAATACGCG AGTCTTTGAA TGATGAAGAA 
ATCGAAGCCA AGCCTAAACA GGCCTCTGGA AACCGTGAAA CTTTTGCGGC TGGTGAAAAT 
ACATGCAGCA AGCCCCAGCT GCCGAACGGT TCCCACTCGT CTGTAGAGGC CAAATCTGAA 
GAAACGATCG ATAGAACATC TATGCCCTCT AAACGCGAAC GAGCTGCAAA TTCTATTAAC 
CCTTTTATCA TCGAAGACGC TAAAGAAAGA TCTGACAAAA CCTTTAAATG CCCTGAAAGT 
AGTTATGTAG CTTCTGATGC TAATCAGGAA ACGGTTCGAA GTGACTGCAA AAACTCCCAA 
ATAAATGGCG AAATTCCCGT TGAGGCCAAT AATGATGAAC CAGCCAAGTC GGCACACAAT 
TTTGCTAGCG AAGATATTGA AAAACCCCCC AAAAGGCTGA AGAGACAGTG CCGAATTAAA 
CCATCTGTGG AGGCTAAAAG TGGGGATAAA CCTAAGAAAC CGGCTAATCT CGAGGAAGAT 
GTAGCAGTAG TCTGTGTAGA GGAAACAAGC AAAGAAGAAA TAGCAAACCC GACTCACATC 
GACCAATCGA TGGCATTGCT GGCTTCAAAA ATGATCAAAA CGAGGAAGAC GACCAAAACT 
GAGGAAATCC GAATCAACTT TCCGAAAGAG AGGAAAAGCA TGGCGTTAAG TGTGGATGAA 
GAGGAGATGC CAAGGAGCCG GCGAGCGGCA GCAGAAGGAA AGCGCGCCAA GACCTCAAAG 
CCGAATGAAA AACCACCAGC AACAAAAGCG GCCAAACGAG CCAATAAAGA ACGGGCCGCA 
GATGAAGGGA GCTTATCCAA GAAACAGAGG CTTCAATCAG AAGACTCACC GAGAGGAAAC 
CTGCGGAAAC AACGCCACAA AGTGGTCTTC ACCATGCTGA ACAGCCCCCA ATTGGAATCT 
CTGATCAAGC AACTAGGCGG CAGCATTGTG GACTCGGTGG ACTCTTGCAC CGTTCTGATC 
ACTGAGCACG TGAAAAGGTC TCAGAAGCTC CTAGAAGCCA TTGGCAGGGG ACGACCGATC 
TGCTCACCTC AATGGGTATT GGACTCGAAA CGCGCCAAGC AGTTCCTAGA TCCTTGGGAC 
TATATCCTGA CCGATGCGGA AGCGGAGACA AATTGGGAAT TTTCTCTGAA CGAGTCGTTG 
CAACGGAGCA TGGCGCAGAA CCTGCTGAAG GACTACAACT TCTATATCCA AGTGACCAAT 
GGAGTGGACG TCCTTAAAGG AGGAATTAAG GCCTGCGGCG GCAAATGTCT GGCGCGGATC 
CCATCCGGGC CCCTTCAGCA CGCCTATGTG ATTTCGACGG TCGAGAATCG CACAAAACAC 
AACAAAATCC TGCAGAAACA TCCGCATTTC CAGGCCATAG AAGCCGAAGC TGTGTTTGAT 
GCGATTTTGA GGCAGGAAAT CCGATTTACT CGGCATATTA TAAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019773274.1
CDS	134..3301
Translation	MQPPGIGPQMGLFTINNQKFPLYEGLNTIGRNREAVVNIKHVLISQHHALITVLDGKFFISDLKSSNGSFLEGMRLVPLKMYPFNNDAKITLGNLHGTFTITSDPPLEERLIETQTQSLFDGGTQLMQSTGNVQEELTQKILWPPSVPTYVPGLQIGEIHDEPLSVLENYVELEPDPQLERVEDLPSSPVVISDDEVTDCEENPGPSGEHDANNHEATFCDKSNESLEFLNQSPTTAQPNGEESRDSSDDDSFAFKRTRKSFMVISTQDLQENASESSSHQLEETIPATQYPSALELNESDIIAATQALRPDSQSSESEFFKLGLTQLMDSSLNGITSQAAEPAPEENESIDTTHVSLAQAVQNNEDIYEVATQKVASTADETFNIFDAPTQKLRTVEDSFLQPTQPMFSEALEDVDYDSPTQKVSDDDDTFLAPTQKLSFSAAAPKENEDFFLAPTQRVLRPKRVYSFKKTKCNLDASLSVLAVGEAETGQAKLDSAATGKKTDDEVIAQLDKETFMDSPTRDISKPQDAEDVEGLQAKQDTATGAKGEDYFKTSNAVKLQAKKSESHVKIRESLNDEEIEAKPKQASGNRETFAAGENTCSKPQLPNGSHSSVEAKSEETIDRTSMPSKRERAANSINPFIIEDAKERSDKTFKCPESSYVASDANQETVRSDCKNSQINGEIPVEANNDEPAKSAHNFASEDIEKPPKRLKRQCRIKPSVEAKSGDKPKKPANLEEDVAVVCVEETSKEEIANPTHIDQSMALLASKMIKTRKTTKTEEIRINFPKERKSMALSVDEEEMPRSRRAAAEGKRAKTSKPNEKPPATKAAKRANKERAADEGSLSKKQRLQSEDSPRGNLRKQRHKVVFTMLNSPQLESLIKQLGGSIVDSVDSCTVLITEHVKRSQKLLEAIGRGRPICSPQWVLDSKRAKQFLDPWDYILTDAEAETNWEFSLNESLQRSMAQNLLKDYNFYIQVTNGVDVLKGGIKACGGKCLARIPSGPLQHAYVISTVENRTKHNKILQKHPHFQAIEAEAVFDAILRQEIRFTRHIIS

Target ORF information:

RefSeq Version	XM_019917715.1
Organism	Dendroctonus ponderosae(mountain pine beetle)
Definition	Dendroctonus ponderosae mediator of DNA damage checkpoint protein 1-like (LOC109546665), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019917715.1

ORF Insert Sequence:

ATGCAGCCGC CGGGCATTGG CCCGCAGATG GGCCTTTTTA CAATAAACAA TCAGAAATTT 
CCTCTTTACG AGGGTCTAAA CACAATAGGA CGCAACAGAG AGGCCGTGGT GAATATCAAG 
CATGTTCTCA TTAGTCAACA TCATGCGCTT ATTACGGTAC TGGACGGGAA GTTTTTCATC 
AGCGACCTGA AATCCAGCAA CGGCAGTTTC CTGGAAGGTA TGCGACTGGT TCCCTTGAAA 
ATGTATCCTT TCAATAACGA CGCCAAAATC ACCCTGGGGA ACTTGCACGG AACCTTCACT 
ATCACATCGG ATCCCCCCCT TGAGGAGCGA TTGATTGAGA CTCAAACTCA GTCATTATTT 
GATGGAGGCA CCCAACTGAT GCAGTCCACT GGGAATGTTC AGGAAGAACT CACTCAGAAG 
ATTTTGTGGC CCCCGTCGGT TCCCACGTAT GTACCAGGGC TTCAAATCGG CGAAATCCAT 
GATGAGCCTT TGTCGGTTTT GGAAAATTAT GTTGAACTCG AACCAGATCC ACAGTTGGAA 
AGGGTTGAAG ATCTGCCTTC TTCGCCTGTG GTGATCAGCG ACGATGAAGT CACGGACTGT 
GAGGAAAACC CTGGTCCTAG TGGAGAGCAT GATGCAAATA ACCATGAGGC CACGTTTTGT 
GACAAAAGTA ATGAATCTCT GGAGTTTTTG AACCAGTCCC CAACCACGGC ACAGCCTAAT 
GGAGAAGAAA GCCGCGATTC CAGTGATGAT GATTCTTTTG CGTTCAAGCG AACCCGGAAG 
TCTTTTATGG TGATTTCCAC TCAGGATCTT CAAGAAAACG CGAGTGAGTC CAGTAGCCAT 
CAGTTGGAGG AAACGATTCC AGCCACCCAA TATCCATCAG CCCTGGAGTT GAATGAAAGC 
GACATTATTG CGGCCACTCA AGCTTTGCGC CCGGATAGTC AAAGCAGCGA GTCGGAGTTT 
TTCAAATTGG GGCTAACGCA GTTGATGGAT TCTAGCCTGA ACGGGATCAC AAGTCAGGCC 
GCAGAACCAG CACCAGAAGA AAACGAATCA ATCGACACAA CCCACGTGAG CCTCGCTCAG 
GCCGTGCAGA ACAACGAAGA CATCTATGAG GTAGCCACTC AAAAAGTTGC GTCCACTGCC 
GACGAGACGT TCAATATCTT TGACGCTCCC ACTCAAAAAC TCCGCACAGT GGAGGATTCA 
TTTCTTCAAC CCACACAACC GATGTTCTCC GAAGCGCTAG AAGACGTTGA CTATGACAGC 
CCCACTCAGA AAGTCTCTGA CGATGATGAT ACTTTTCTGG CACCCACTCA GAAGCTGAGC 
TTCAGCGCGG CTGCACCCAA AGAGAATGAG GACTTTTTCT TGGCTCCAAC GCAGAGGGTT 
CTGCGTCCGA AACGGGTGTA TTCCTTCAAG AAAACCAAGT GCAATCTAGA TGCTTCGCTT 
TCGGTTTTGG CCGTAGGTGA AGCTGAAACT GGCCAAGCGA AACTCGATTC TGCAGCAACG 
GGGAAGAAAA CCGACGACGA AGTCATTGCT CAACTAGATA AAGAAACATT CATGGATAGT 
CCAACTAGGG ATATTTCCAA GCCTCAAGAT GCCGAAGATG TTGAGGGTTT GCAGGCGAAA 
CAGGACACAG CCACGGGGGC CAAAGGCGAG GATTATTTCA AAACAAGTAA TGCGGTTAAA 
CTGCAAGCGA AGAAAAGTGA AAGCCATGTA AAAATACGCG AGTCTTTGAA TGATGAAGAA 
ATCGAAGCCA AGCCTAAACA GGCCTCTGGA AACCGTGAAA CTTTTGCGGC TGGTGAAAAT 
ACATGCAGCA AGCCCCAGCT GCCGAACGGT TCCCACTCGT CTGTAGAGGC CAAATCTGAA 
GAAACGATCG ATAGAACATC TATGCCCTCT AAACGCGAAC GAGCTGCAAA TTCTATTAAC 
CCTTTTATCA TCGAAGACGC TAAAGAAAGA TCTGACAAAA CCTTTAAATG CCCTGAAAGT 
AGTTATGTAG CTTCTGATGC TAATCAGGAA ACGGTTCGAA GTGACTGCAA AAACTCCCAA 
ATAAATGGCG AAATTCCCGT TGAGGCCAAT AATGATGAAC CAGCCAAGTC GGCACACAAT 
TTTGCTAGCG AAGATATTGA AAAACCCCCC AAAAGGCTGA AGAGACAGTG CCGAATTAAA 
CCATCTGTGG AGGCTAAAAG TGGGGATAAA CCTAAGAAAC CGGCTAATCT CGAGGAAGAT 
GTAGCAGTAG TCTGTGTAGA GGAAACAAGC AAAGAAGAAA TAGCAAACCC GACTCACATC 
GACCAATCGA TGGCATTGCT GGCTTCAAAA ATGATCAAAA CGAGGAAGAC GACCAAAACT 
GAGGAAATCC GAATCAACTT TCCGAAAGAG AGGAAAAGCA TGGCGTTAAG TGTGGATGAA 
GAGGAGATGC CAAGGAGCCG GCGAGCGGCA GCAGAAGGAA AGCGCGCCAA GACCTCAAAG 
CCGAATGAAA AACCACCAGC AACAAAAGCG GCCAAACGAG CCAATAAAGA ACGGGCCGCA 
GATGAAGGGA GCTTATCCAA GAAACAGAGG CTTCAATCAG AAGACTCACC GAGAGGAAAC 
CTGCGGAAAC AACGCCACAA AGTGGTCTTC ACCATGCTGA ACAGCCCCCA ATTGGAATCT 
CTGATCAAGC AACTAGGCGG CAGCATTGTG GACTCGGTGG ACTCTTGCAC CGTTCTGATC 
ACTGAGCACG TGAAAAGGTC TCAGAAGCTC CTAGAAGCCA TTGGCAGGGG ACGACCGATC 
TGCTCACCTC AATGGGTATT GGACTCGAAA CGCGCCAAGC AGTTCCTAGA TCCTTGGGAC 
TATATCCTGA CCGATGCGGA AGCGGAGACA AATTGGGAAT TTTCTCTGAA CGAGTCGTTG 
CAACGGAGCA TGGCGCAGAA CCTGCTGAAG GACTACAACT TCTATATCCA AGTGACCAAT 
GGAGTGGACG TCCTTAAAGG AGGAATTAAG GCCTGCGGCG GCAAATGTCT GGCGCGGATC 
CCATCCGGGC CCCTTCAGCA CGCCTATGTG ATTTCGACGG TCGAGAATCG CACAAAACAC 
AACAAAATCC TGCAGAAACA TCCGCATTTC CAGGCCATAG AAGCCGAAGC TGTGTTTGAT 
GCGATTTTGA GGCAGGAAAT CCGATTTACT CGGCATATTA TAAGTTAG