selenok cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	selenok
Entrez Gene ID	791738
Full Name	selenoprotein K
Synonyms	selk,si:ch1073-219o6.1,zgc:103591
General protein information	Preferred Names selenoprotein K Names selenoprotein K
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)
Summary	The protein encoded by this gene belongs to the selenoprotein K family. It is a transmembrane protein that is localized in the endoplasmic reticulum (ER), and is involved in ER-associated degradation (ERAD) of misfolded, glycosylated proteins. It also has a role in the protection of cells from ER stress-induced apoptosis. Like all selenoproteins, this protein contains the rare amino acid selenocysteine (Sec), which is encoded by the UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. [provided by RefSeq, Aug 2017].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001004681.3	NP_001004681.2	selenoprotein K

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Selk	NP_064363.2
Rattus norvegicus (Norway rat)	Selk	NP_997472.2
Gallus gallus (chicken)	SELK	NP_001020612.1
Canis lupus familiaris (dog)	SELK	NP_001108350.1
Bos taurus (cattle)	SELK	NP_001032566.2
	SELK	NP_001152853.1
Homo sapiens (human)	SELK	NP_067060.2
Pan troglodytes (chimpanzee)	SELK	NP_001108349.1
Danio rerio (zebrafish)	selk	NP_001004681.2

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Selenoprotein Gene Nomenclature.
Gladyshev VN, Arn?r ES, Berry MJ, Brigelius-Floh? R, Bruford EA, Burk RF, Carlson BA, Castellano S, Chavatte L, Conrad M, Copeland PR, Diamond AM, Driscoll DM, Ferreiro A, Floh? L, Green FR, Guig? R, Handy DE, Hatfield DL, Hesketh J, Hoffmann PR, Holmgren A, Hondal RJ, Howard MT, Huang K, Kim HY, Kim IY, K?hrle J, Krol A, Kryukov GV, Lee BJ, Lee BC, Lei XG, Liu Q, Lescure A, Lobanov AV, Loscalzo J, Maiorino M, Mariotti M, Sandeep Prabhu K, Rayman MP, Rozovsky S, Salinas G, Schmidt EE, Schomburg L, Schweizer U, Simonovi? M, Sunde RA, Tsuji PA, Tweedie S, Ursini F, Whanger PD, Zhang Y
The Journal of biological chemistry291(46)24036-24040(2016 Nov)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Selenium prevents downregulation of antioxidant selenoprotein genes by methylmercury.
Penglase S, Hamre K, Ellingsen S
Free radical biology & medicine7595-104(2014 Oct)

Composition and evolution of the vertebrate and mammalian selenoproteomes.
Mariotti M, Ridge PG, Zhang Y, Lobanov AV, Pringle TH, Guigo R, Hatfield DL, Gladyshev VN
PloS one7(3)e33066(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following selenok gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the selenok cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa00832	NM_001004681.3 Latest version!	Danio rerio selenoprotein K (selenok), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

selenok ( NM_001004681.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001004681.2 Danio rerio selenoprotein K (selenok), mRNA.

Price & Availability↑

CloneID	ODa00832
Clone ID Related Accession (Same CDS sequence)	NM_001004681.3
Accession Version	NM_001004681.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 285bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Danio rerio(zebrafish)
Product	selenoprotein K
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EH505888.1, EH506691.1, EH471010.1 and CU855933.8. On Dec 17, 2014 this sequence version replaced NM_001004681.2. ##Evidence-Data-START## Transcript exon combination :: GFIL01034747.1, GFIL01034746.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505373, SAMEA3505384 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGTTTATG TGTCAAATGG TCAGGTTCTG GACAGCAGGA GCCGTTCGCC CTGGAGTCTG 
TCCTTCCTGA CCGACTTCTT CTGGGGTGCA GTCGAGTTCA TCGGCCTGTT CTTCCAGACT 
CTGGTCCAGC CGGATCTGTC TAAAGATGGA AACAACAGCG CATCGTCTCG CTTCAGTGAC 
GGCAGAGGTC CTCCTGGGTT CCCAGGCCGC CGACGGATGG GTAGAATAAA CCACGGTGCA 
GGTCCCACAC CTCCACCCAT GGGCGGAGGA GGATGAGGAA GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001004681.2
CDS	38..322
Translation	MVYVSNGQVLDSRSRSPWSLSFLTDFFWGAVEFIGLFFQTLVQPDLSKDGNNSASSRFSDGRGPPGFPGRRRMGRINHGAGPTPPPMGGGG*GR

Target ORF information:

RefSeq Version	NM_001004681.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio selenoprotein K (selenok), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001004681.3

ORF Insert Sequence:

ATGGTTTATG TGTCAAATGG TCAGGTTCTG GACAGCAGGA GCCGTTCGCC CTGGAGTCTG 
TCCTTCCTGA CCGACTTCTT CTGGGGTGCA GTCGAGTTCA TCGGCCTGTT CTTCCAGACT 
CTGGTCCAGC CGGATCTGTC TAAAGATGGA AACAACAGCG CATCGTCTCG CTTCAGTGAC 
GGCAGAGGTC CTCCTGGGTT CCCAGGCCGC CGACGGATGG GTAGAATAAA CCACGGTGCA 
GGTCCCACAC CTCCACCCAT GGGCGGAGGA GGATGAGGAA GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Selenoprotein Gene Nomenclature.
The Journal of biological chemistry291(46)24036-24040(2016 Nov)
Gladyshev VN,Arn?r ES,Berry MJ,Brigelius-Floh? R,Bruford EA,Burk RF,Carlson BA,Castellano S,Chavatte L,Conrad M,Copeland PR,Diamond AM,Driscoll DM,Ferreiro A,Floh? L,Green FR,Guig? R,Handy DE,Hatfield DL,Hesketh J,Hoffmann PR,Holmgren A,Hondal RJ,Howard MT,Huang K,Kim HY,Kim IY,K?hrle J,Krol A,Kryukov GV,Lee BJ,Lee BC,Lei XG,Liu Q,Lescure A,Lobanov AV,Loscalzo J,Maiorino M,Mariotti M,Sandeep Prabhu K,Rayman MP,Rozovsky S,Salinas G,Schmidt EE,Schomburg L,Schweizer U,Simonovi? M,Sunde RA,Tsuji PA,Tweedie S,Ursini F,Whanger PD,Zhang Y

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Selenium prevents downregulation of antioxidant selenoprotein genes by methylmercury.
Free radical biology & medicine7595-104(2014 Oct)
Penglase S,Hamre K,Ellingsen S

Composition and evolution of the vertebrate and mammalian selenoproteomes.
PloS one7(3)e33066(2012)
Mariotti M,Ridge PG,Zhang Y,Lobanov AV,Pringle TH,Guigo R,Hatfield DL,Gladyshev VN

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

selenok cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報