cmlc1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	cmlc1
Entrez Gene ID	64671
Full Name	cardiac myosin light chain-1
Synonyms	zgc:103441
General protein information	Preferred Names cardiac myosin light chain-1 Names cardiac myosin light chain-1 laz lazy susan
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_131692.1	NP_571767.1	cardiac myosin light chain-1
NM_131692.2	NP_571767.2	cardiac myosin light chain-1

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	K04C1.4	NP_510375.2
Danio rerio (zebrafish)	cmlc1	NP_571767.1

Gene Ontology
Bibliography

Related articles in PubMed

Cardio-respirometry disruption in zebrafish (Danio rerio) embryos exposed to hydraulic fracturing flowback and produced water.
Folkerts EJ, Blewett TA, He Y, Goss GG
Environmental pollution (Barking, Essex : 1987)231(Pt 2)1477-1487(2017 Dec)

Re-evaluating the functional landscape of the cardiovascular system during development.
Takada N, Omae M, Sagawa F, Chi NC, Endo S, Kozawa S, Sato TN
Biology open6(11)1756-1770(2017 Nov)

Claudin5a is required for proper inflation of Kupffer's vesicle lumen and organ laterality.
Kim JG, Bae SJ, Lee HS, Park JH, Kim KW
PloS one12(8)e0182047(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Using adult heterozygous zebrafish, show that ELC S195 phosphorylation is pivotal for adaptation of cardiac function to augmented physical stress and provide novel mechanistic insights into the pathogenesis of ELC-linked cardiomyopathy.
Title: Essential light chain S195 phosphorylation is required for cardiac adaptation under physical stress.

A single serine in the carboxyl terminus of cardiac essential myosin light chain-1 controls cardiomyocyte contractility in vivo.
Title: A single serine in the carboxyl terminus of cardiac essential myosin light chain-1 controls cardiomyocyte contractility in vivo.

There are distinct roles for cmlc1 and cmlc2 during zebrafish cardiogenesis.
Title: Depletion of zebrafish essential and regulatory myosin light chains reduces cardiac function through distinct mechanisms.

The following cmlc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cmlc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa38442	NM_131692.1 Latest version!	Danio rerio cardiac myosin light chain-1 (cmlc1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
ODa141172	NM_131692.2 Latest version!	Danio rerio cardiac myosin light chain-1 (cmlc1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cmlc1 ( NM_131692.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571767.1 Danio rerio cardiac myosin light chain-1 (cmlc1), mRNA.

Price & Availability↑

CloneID	ODa38442
Clone ID Related Accession (Same CDS sequence)	NM_131692.1
Accession Version	NM_131692.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-01-31
Organism	Danio rerio(Zebrafish)
Product	cardiac myosin light chain-1
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC083381.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC083381.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCACCAA AAAAAGTGGA ACCCAAGAAA CCAGAGCCGA AAAAACCTGA GGCCAAAAAA 
GAAGAAGCCC CTGCTCCTGC ACCAGTTCCA GAGACACCCA AAGAGCCCGA AGTGGACCTG 
AAAAGCGTCC CGCTGGATTT TAGCCCAGAC CAGATTGAAG AGTTCAGGGA TGCCTTTACT 
CTCTTTGATG AAACGCCAAC AGGAGAGATG AAGATAAGGT ATGCCCAGTG TGGAGATGTG 
ATGAGGGCTC TGGGTCACAA CCCTACAAAT GCTGATGTAT TGACGGTATT AGGGAAGCCC 
AAAGCTGAAG AAATGAACAC CAAATACCTG GACTTTGAGA CGTTCCTGCC AATGCTGCAG 
CATGTCTCAA GAGCCAAGGA CCAGGGGACG TTTGAGGACT TTGTTGAGGG TCTGAGAGTC 
TTTGACAAAG AGGGAAACGG AACAGTGATG GGTGCTGAGC TCCGTCACGT CTTGGCAACG 
CTTGGGGAGA AAATGACAGA GGATGAGGCG GACCGTCTGA TGGCCGGACA AGAAGACGCC 
AACGGCTGCA TTAACTACAC GTCCTTCATC AAGCACATCC TCTCCGGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571767.1
CDS	83..673
Translation	MAPKKVEPKKPEPKKPEAKKEEAPAPAPVPETPKEPEVDLKSVPLDFSPDQIEEFRDAFTLFDETPTGEMKIRYAQCGDVMRALGHNPTNADVLTVLGKPKAEEMNTKYLDFETFLPMLQHVSRAKDQGTFEDFVEGLRVFDKEGNGTVMGAELRHVLATLGEKMTEDEADRLMAGQEDANGCINYTSFIKHILSG

Target ORF information:

RefSeq Version	NM_131692.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio cardiac myosin light chain-1 (cmlc1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131692.1

ORF Insert Sequence:

ATGGCACCAA AAAAAGTGGA ACCCAAGAAA CCAGAGCCGA AAAAACCTGA GGCCAAAAAA 
GAAGAAGCCC CTGCTCCTGC ACCAGTTCCA GAGACACCCA AAGAGCCCGA AGTGGACCTG 
AAAAGCGTCC CGCTGGATTT TAGCCCAGAC CAGATTGAAG AGTTCAGGGA TGCCTTTACT 
CTCTTTGATG AAACGCCAAC AGGAGAGATG AAGATAAGGT ATGCCCAGTG TGGAGATGTG 
ATGAGGGCTC TGGGTCACAA CCCTACAAAT GCTGATGTAT TGACGGTATT AGGGAAGCCC 
AAAGCTGAAG AAATGAACAC CAAATACCTG GACTTTGAGA CGTTCCTGCC AATGCTGCAG 
CATGTCTCAA GAGCCAAGGA CCAGGGGACG TTTGAGGACT TTGTTGAGGG TCTGAGAGTC 
TTTGACAAAG AGGGAAACGG AACAGTGATG GGTGCTGAGC TCCGTCACGT CTTGGCAACG 
CTTGGGGAGA AAATGACAGA GGATGAGGCG GACCGTCTGA TGGCCGGACA AGAAGACGCC 
AACGGCTGCA TTAACTACAC GTCCTTCATC AAGCACATCC TCTCCGGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cmlc1 ( NM_131692.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571767.2 Danio rerio cardiac myosin light chain-1 (cmlc1), mRNA.

Price & Availability↑

CloneID	ODa141172
Clone ID Related Accession (Same CDS sequence)	NM_131692.2
Accession Version	NM_131692.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-21
Organism	Danio rerio(zebrafish)
Product	cardiac myosin light chain-1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL845516.8. On May 24, 2017 this sequence version replaced NM_131692.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: BC083381.1 [ECO:0000345] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCACCAA AAAAAGTGGA ACCCAAGAAA CCAGAGCCGA AAAAACCTGA GGCCAAAAAA 
GAAGAAGCCC CTGCTCCTGC ACCAGTTCCA GAGACACCCA AAGAGCCCGA AGTGGACCTG 
AAAAGCGTCC CGCTGGATTT TAGCCCAGAC CAGATTGAAG AGTTCAGGGA TGCCTTTACT 
CTCTTTGATG AAACGCCAAC AGGAGAGATG AAGATAAGGT ATGCCCAGTG TGGAGATGTG 
ATGAGGGCTC TGGGTCACAA CCCTACCAAT GCTGATGTAT TGACGGTATT AGGGAAGCCC 
AAAGCTGAAG AAATGAACAC CAAATACCTG GACTTTGAGA CGTTCCTGCC AATGCTGCAG 
CATATCTCAA GAGCCAAGGA CCAGGGGACG TTTGAGGACT TTGTTGAGGG TCTGAGAGTC 
TTTGACAAAG AGGGAAACGG AACAGTGATG GGTGCTGAGC TCCGTCACGT CTTGGCAACG 
CTTGGGGAGA AAATGACAGA GGATGAGGTG GACCGTCTGA TGGCCGGACA AGAAGACGCC 
AACGGCTGCA TTAACTACAC GTCCTTCATC AAGCACATCC TCTCCGGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571767.2
CDS	82..672
Translation	MAPKKVEPKKPEPKKPEAKKEEAPAPAPVPETPKEPEVDLKSVPLDFSPDQIEEFRDAFTLFDETPTGEMKIRYAQCGDVMRALGHNPTNADVLTVLGKPKAEEMNTKYLDFETFLPMLQHISRAKDQGTFEDFVEGLRVFDKEGNGTVMGAELRHVLATLGEKMTEDEVDRLMAGQEDANGCINYTSFIKHILSG

Target ORF information:

RefSeq Version	NM_131692.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio cardiac myosin light chain-1 (cmlc1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131692.2

ORF Insert Sequence:

ATGGCACCAA AAAAAGTGGA ACCCAAGAAA CCAGAGCCGA AAAAACCTGA GGCCAAAAAA 
GAAGAAGCCC CTGCTCCTGC ACCAGTTCCA GAGACACCCA AAGAGCCCGA AGTGGACCTG 
AAAAGCGTCC CGCTGGATTT TAGCCCAGAC CAGATTGAAG AGTTCAGGGA TGCCTTTACT 
CTCTTTGATG AAACGCCAAC AGGAGAGATG AAGATAAGGT ATGCCCAGTG TGGAGATGTG 
ATGAGGGCTC TGGGTCACAA CCCTACCAAT GCTGATGTAT TGACGGTATT AGGGAAGCCC 
AAAGCTGAAG AAATGAACAC CAAATACCTG GACTTTGAGA CGTTCCTGCC AATGCTGCAG 
CATATCTCAA GAGCCAAGGA CCAGGGGACG TTTGAGGACT TTGTTGAGGG TCTGAGAGTC 
TTTGACAAAG AGGGAAACGG AACAGTGATG GGTGCTGAGC TCCGTCACGT CTTGGCAACG 
CTTGGGGAGA AAATGACAGA GGATGAGGTG GACCGTCTGA TGGCCGGACA AGAAGACGCC 
AACGGCTGCA TTAACTACAC GTCCTTCATC AAGCACATCC TCTCCGGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cardio-respirometry disruption in zebrafish (Danio rerio) embryos exposed to hydraulic fracturing flowback and produced water.
Environmental pollution (Barking, Essex : 1987)231(Pt 2)1477-1487(2017 Dec)
Folkerts EJ,Blewett TA,He Y,Goss GG

Re-evaluating the functional landscape of the cardiovascular system during development.
Biology open6(11)1756-1770(2017 Nov)
Takada N,Omae M,Sagawa F,Chi NC,Endo S,Kozawa S,Sato TN

Claudin5a is required for proper inflation of Kupffer's vesicle lumen and organ laterality.
PloS one12(8)e0182047(2017)
Kim JG,Bae SJ,Lee HS,Park JH,Kim KW

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cmlc1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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