actn3a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	actn3a
Entrez Gene ID	58037
Full Name	actinin alpha 3a
Synonyms	ACTN2,actn3,actnl,zgc:63559
General protein information	Preferred Names actinin alpha 3a Names actinin alpha 3a actinin alpha 3
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_131522.2	NP_571597.1	actinin alpha 3a

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ACTN3	XP_005631486.1
Anopheles gambiae (African malaria mosquito)	ACTN_ANOGA	XP_321625.2
Bos taurus (cattle)	ACTN3	NP_001069625.1
Mus musculus (house mouse)	Actn3	NP_038484.1
Pan troglodytes (chimpanzee)	ACTN3	XP_003313215.1
	ACTN3	XP_001109839.1
Danio rerio (zebrafish)	actn3b	NP_991107.1
Drosophila melanogaster (fruit fly)	Actn	NP_477485.1
Rattus norvegicus (Norway rat)	Actn3	NP_596915.1
Danio rerio (zebrafish)	actn3a	NP_571597.1
Caenorhabditis elegans (roundworm)	atn-1	NP_506128.1
Xenopus tropicalis (tropical clawed frog)	actn3	NP_001135513.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RNA splicing regulated by RBFOX1 is essential for cardiac function in zebrafish.
Frese KS, Meder B, Keller A, Just S, Haas J, Vogel B, Fischer S, Backes C, Matzas M, K?hler D, Benes V, Katus HA, Rottbauer W
Journal of cell science128(16)3030-40(2015 Aug)

New classes of mind bomb-interacting proteins identified from yeast two-hybrid screens.
Tseng LC, Zhang C, Cheng CM, Xu H, Hsu CH, Jiang YJ
PloS one9(4)e93394(2014)

?-Actinin2 is required for the lateral alignment of Z discs and ventricular chamber enlargement during zebrafish cardiogenesis.
Yang J, Xu X
FASEB journal : official publication of the Federation of American Societies for Experimental Biology26(10)4230-42(2012 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Duplication of actn3 in the zebrafish indicates that variation in actn3 expression may promote physiological diversity in muscle function among vertebrates.
Title: Sequence and expression of the zebrafish alpha-actinin gene family reveals conservation and diversification among vertebrates.

The following actn3a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the actn3a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa33365	NM_131522.2 Latest version!	Danio rerio actinin alpha 3a (actn3a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

actn3a ( NM_131522.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_571597.1 Danio rerio actinin alpha 3a (actn3a), mRNA.

Price & Availability↑

CloneID	ODa33365
Clone ID Related Accession (Same CDS sequence)	NM_131522.2
Accession Version	NM_131522.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2691bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	actinin alpha 3a
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC054911.1. On Aug 31, 2012 this sequence version replaced NM_131522.1. ##Evidence-Data-START## Transcript exon combination :: BC054911.1, AY391405.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505370, SAMEA3505371 [ECO:0000348] ##Evidence-Data-END##

ATGACAGCGG TTGAATCGCA AGTGCAGTAC GGGTCTTACA TGATGACCGC GACTGAGGAA 
TACATGATCC AAGAGGACGA CTGGGACCGA GACCTGTTCC TGGACCCGGC CTGGGAGAAG 
CAGCAGAGGA AGACCTTCAC TGCTTGGTGC AACTCTCACC TGCGTAAGGC AGGGACACAG 
ATTGAAAATA TTGAGGAGGA CTTCAGGAAT GGACTCAAGC TCATGCTGCT GCTGGAGGCT 
ATCTCAGGTG AGAGACTCCC TAAACCAGAC AAAGGCAAAA TGCGTTTTCA CAAGATCGCC 
AATGTGAACA AGGCCCTAGA CTTCATTAGC AGCAAGGGAG TCAAGCTGGT GTCCATCGGT 
GCTGAGGAGA TTGTGGATGG GAATCTGAAA ATGACCCTAG GTATGATCTG GACCATCATC 
CTGCGCTTTG CAATCCAGGA CATTTCAGTG GAAGAGACCT CTGCTAAGGA GGGCTTGCTG 
CTGTGGTGTC AGAGGAAGAC TGCCCCCTAC AGGAACGTCA ACGTGCAGAA CTTCCACATC 
AGTTGGAAGG ATGGCCTGGC ACTTTGTGCC CTCATCCACA GACACAGACC TGACCTCATC 
GACTACTCCA AACTGCGCAA GGATGACCCA ATTGGCAATC TCAACACTGC TTTTGATATT 
GCAGAGAAAT TCCTTGACAT CCCTAAAATG CTTGATGCAG ATGACATTGT GAACACTCCA 
AAACCTGATG AGAAGGCCAT CATGACCTAT GTGTCATGTT TCTACCATGC TTTTGCTGGT 
GCAGAGCAGG CTGAGACAGC AGCTAACAGG ATCTGCAAAG TCCTGGCAGT GAACCAGGAA 
AATGAGAAGC TGATGGAAGA ATACGAGAAA CTGGCCAGCG AGCTTCTGGA GTGGATTCAA 
AGGACCATTC CCTGGTTAGA GAACCGTGTG GCTGAGCAGA CCATGCATGC CATGCAGCAG 
AAGCTAGAGG ATTTTAGGGA TTATCGTCGT GTGCATAAGC CACCCAAGGT TCAAGAGAAG 
TGTCAGCTGG AGATCAACTT CAACACCCTA CAGACCAAGC TAAGGCTCAG CAACAGGCCT 
GCCTTCATGC CTTCCGAGGG CAAAATGGTT TCAGATATCG CCGATGCTTG GAAGGGTCTG 
GAGCAGGTTG AAAAGGGCTA TGAGGAGTGG CTGCTTACAG AAATTCGTCG TCTAGAGAGA 
CTGGACCACC TGGCTGAGAA GTTTAGGCAG AAGTCAACCC TACACCAATC GTGGACCACT 
GGAAAGGAGG AGCTTCTGTC TCAAAAGGAC TACGAAACAG CCTCTCTGAT GGAGATTCGT 
GCTTTGATGA GGAAACACGA GGCCTTTGAG AGTGACTTGG CTGCTCACCA GGACAGAGTA 
GAGCAGATCG CTGCCATTGC ACAGGAGCTC AATGAATTGG ACTTTTATGA TGCTGCCACC 
ATCAACGCCC AATGCCAGGG CATCTGTGAC CAGTGGGACA ATCTGGGCAC CCTGACTCAG 
AAAAGAAGAG AATCACTGGA ACGTGTGGAG AAACTTTGGG AGACAATTGA CCAGTTGTAT 
TTGGAGTTTG CCAAGAGGGC AGCACCCTTC AACAACTGGA TGGATGGGGC TATGGAAGAT 
CTGCAGGACA TGTTCATTGT GCACAGTATT GAGGAGATTC AGAGTTTGAT CACAGCACAT 
GACCAATTCA AGGCAACCCT ACCAGAAGCT GATAAGGAGC GCATGGCTAT CATGGGCATT 
CATAGTGAAG TCCTGAAGAT AGCTCAGACT TATGGAATCA AGATAGTGAG TGAGAACCCC 
TACACCGTGC TCTCTCATCA GAATATTGTC AATAAATGGG AAGCTGTGAA GCAACTGGTT 
CCAATGCGTG ACCAGATGTT GCAGGAGGAA GTGGCCAGAC AGCAGTCCAA TGAGAGGCTG 
AGGCGCCAAT TTGCTGCTCA GGCCAATATC ATTGGACCTT GGATCCAAAC CAAGATGGAG 
GAGATCAGCC ATGTGTCTAT TGACATTGCT GGTTCCCTTG AAGAACAGAT GAGCAATCTT 
AAAACATATG AACAGAATAT CATTAATTAC AAATCCAACA TTGACAAACT GGAGGGCGAC 
CACCAGCTTA GCCAGGAGGG ACTTATCTTT GACAACAAGC ACACCAACTA CACTATGGAG 
CATGTCCGTG TGGGATGGGA GCAGCTCCTC ACCACCATCG CTCGCACAAT CAATGAGGTT 
GAGAACCAGA TCCTGACTCG TGATGCCAAG GGCATCAGTC AGGAGCAGCT GAATGAGTTC 
AGAGCCTCAT TCAATCACTT TGACCGGAAG AGAAATGGCA TGATGGATCC CGATGACTTC 
CGTGCTTGCC TGATTTCTAT GGGTTATGAT TTGGGTGAAG TAGAATTTGC ACGCATTATG 
ACTCTGGTGG ACCCCAACAA CACAGGAGTT GTCACCTTCC AAGCCTTCAT TGACTTCATG 
ACCAGAGAGA CAGCCGAGAC AGACACCGCT GAACAGGTCA TGGCCTCCTT CAAGATTCTG 
GCTTCTGACA AGGCGTACAT TACAGTGGAG GAGCTGAGAA GGGAACTGCC ACCAGAACAA 
GCCGAATACT GTATCAGCCG CATGACCAAG TACATGGGCG GTGATGCTCC TCCAGGCGCG 
CTTGATTATA TCTCCTTCTC AAGTGCTCTG TATGGAGAAA GTGACTTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571597.1
CDS	148..2838
Translation	MTAVESQVQYGSYMMTATEEYMIQEDDWDRDLFLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRNGLKLMLLLEAISGERLPKPDKGKMRFHKIANVNKALDFISSKGVKLVSIGAEEIVDGNLKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYRNVNVQNFHISWKDGLALCALIHRHRPDLIDYSKLRKDDPIGNLNTAFDIAEKFLDIPKMLDADDIVNTPKPDEKAIMTYVSCFYHAFAGAEQAETAANRICKVLAVNQENEKLMEEYEKLASELLEWIQRTIPWLENRVAEQTMHAMQQKLEDFRDYRRVHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGKMVSDIADAWKGLEQVEKGYEEWLLTEIRRLERLDHLAEKFRQKSTLHQSWTTGKEELLSQKDYETASLMEIRALMRKHEAFESDLAAHQDRVEQIAAIAQELNELDFYDAATINAQCQGICDQWDNLGTLTQKRRESLERVEKLWETIDQLYLEFAKRAAPFNNWMDGAMEDLQDMFIVHSIEEIQSLITAHDQFKATLPEADKERMAIMGIHSEVLKIAQTYGIKIVSENPYTVLSHQNIVNKWEAVKQLVPMRDQMLQEEVARQQSNERLRRQFAAQANIIGPWIQTKMEEISHVSIDIAGSLEEQMSNLKTYEQNIINYKSNIDKLEGDHQLSQEGLIFDNKHTNYTMEHVRVGWEQLLTTIARTINEVENQILTRDAKGISQEQLNEFRASFNHFDRKRNGMMDPDDFRACLISMGYDLGEVEFARIMTLVDPNNTGVVTFQAFIDFMTRETAETDTAEQVMASFKILASDKAYITVEELRRELPPEQAEYCISRMTKYMGGDAPPGALDYISFSSALYGESDL

Target ORF information:

RefSeq Version	NM_131522.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio actinin alpha 3a (actn3a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131522.2

ORF Insert Sequence:

ATGACAGCGG TTGAATCGCA AGTGCAGTAC GGGTCTTACA TGATGACCGC GACTGAGGAA 
TACATGATCC AAGAGGACGA CTGGGACCGA GACCTGTTCC TGGACCCGGC CTGGGAGAAG 
CAGCAGAGGA AGACCTTCAC TGCTTGGTGC AACTCTCACC TGCGTAAGGC AGGGACACAG 
ATTGAAAATA TTGAGGAGGA CTTCAGGAAT GGACTCAAGC TCATGCTGCT GCTGGAGGCT 
ATCTCAGGTG AGAGACTCCC TAAACCAGAC AAAGGCAAAA TGCGTTTTCA CAAGATCGCC 
AATGTGAACA AGGCCCTAGA CTTCATTAGC AGCAAGGGAG TCAAGCTGGT GTCCATCGGT 
GCTGAGGAGA TTGTGGATGG GAATCTGAAA ATGACCCTAG GTATGATCTG GACCATCATC 
CTGCGCTTTG CAATCCAGGA CATTTCAGTG GAAGAGACCT CTGCTAAGGA GGGCTTGCTG 
CTGTGGTGTC AGAGGAAGAC TGCCCCCTAC AGGAACGTCA ACGTGCAGAA CTTCCACATC 
AGTTGGAAGG ATGGCCTGGC ACTTTGTGCC CTCATCCACA GACACAGACC TGACCTCATC 
GACTACTCCA AACTGCGCAA GGATGACCCA ATTGGCAATC TCAACACTGC TTTTGATATT 
GCAGAGAAAT TCCTTGACAT CCCTAAAATG CTTGATGCAG ATGACATTGT GAACACTCCA 
AAACCTGATG AGAAGGCCAT CATGACCTAT GTGTCATGTT TCTACCATGC TTTTGCTGGT 
GCAGAGCAGG CTGAGACAGC AGCTAACAGG ATCTGCAAAG TCCTGGCAGT GAACCAGGAA 
AATGAGAAGC TGATGGAAGA ATACGAGAAA CTGGCCAGCG AGCTTCTGGA GTGGATTCAA 
AGGACCATTC CCTGGTTAGA GAACCGTGTG GCTGAGCAGA CCATGCATGC CATGCAGCAG 
AAGCTAGAGG ATTTTAGGGA TTATCGTCGT GTGCATAAGC CACCCAAGGT TCAAGAGAAG 
TGTCAGCTGG AGATCAACTT CAACACCCTA CAGACCAAGC TAAGGCTCAG CAACAGGCCT 
GCCTTCATGC CTTCCGAGGG CAAAATGGTT TCAGATATCG CCGATGCTTG GAAGGGTCTG 
GAGCAGGTTG AAAAGGGCTA TGAGGAGTGG CTGCTTACAG AAATTCGTCG TCTAGAGAGA 
CTGGACCACC TGGCTGAGAA GTTTAGGCAG AAGTCAACCC TACACCAATC GTGGACCACT 
GGAAAGGAGG AGCTTCTGTC TCAAAAGGAC TACGAAACAG CCTCTCTGAT GGAGATTCGT 
GCTTTGATGA GGAAACACGA GGCCTTTGAG AGTGACTTGG CTGCTCACCA GGACAGAGTA 
GAGCAGATCG CTGCCATTGC ACAGGAGCTC AATGAATTGG ACTTTTATGA TGCTGCCACC 
ATCAACGCCC AATGCCAGGG CATCTGTGAC CAGTGGGACA ATCTGGGCAC CCTGACTCAG 
AAAAGAAGAG AATCACTGGA ACGTGTGGAG AAACTTTGGG AGACAATTGA CCAGTTGTAT 
TTGGAGTTTG CCAAGAGGGC AGCACCCTTC AACAACTGGA TGGATGGGGC TATGGAAGAT 
CTGCAGGACA TGTTCATTGT GCACAGTATT GAGGAGATTC AGAGTTTGAT CACAGCACAT 
GACCAATTCA AGGCAACCCT ACCAGAAGCT GATAAGGAGC GCATGGCTAT CATGGGCATT 
CATAGTGAAG TCCTGAAGAT AGCTCAGACT TATGGAATCA AGATAGTGAG TGAGAACCCC 
TACACCGTGC TCTCTCATCA GAATATTGTC AATAAATGGG AAGCTGTGAA GCAACTGGTT 
CCAATGCGTG ACCAGATGTT GCAGGAGGAA GTGGCCAGAC AGCAGTCCAA TGAGAGGCTG 
AGGCGCCAAT TTGCTGCTCA GGCCAATATC ATTGGACCTT GGATCCAAAC CAAGATGGAG 
GAGATCAGCC ATGTGTCTAT TGACATTGCT GGTTCCCTTG AAGAACAGAT GAGCAATCTT 
AAAACATATG AACAGAATAT CATTAATTAC AAATCCAACA TTGACAAACT GGAGGGCGAC 
CACCAGCTTA GCCAGGAGGG ACTTATCTTT GACAACAAGC ACACCAACTA CACTATGGAG 
CATGTCCGTG TGGGATGGGA GCAGCTCCTC ACCACCATCG CTCGCACAAT CAATGAGGTT 
GAGAACCAGA TCCTGACTCG TGATGCCAAG GGCATCAGTC AGGAGCAGCT GAATGAGTTC 
AGAGCCTCAT TCAATCACTT TGACCGGAAG AGAAATGGCA TGATGGATCC CGATGACTTC 
CGTGCTTGCC TGATTTCTAT GGGTTATGAT TTGGGTGAAG TAGAATTTGC ACGCATTATG 
ACTCTGGTGG ACCCCAACAA CACAGGAGTT GTCACCTTCC AAGCCTTCAT TGACTTCATG 
ACCAGAGAGA CAGCCGAGAC AGACACCGCT GAACAGGTCA TGGCCTCCTT CAAGATTCTG 
GCTTCTGACA AGGCGTACAT TACAGTGGAG GAGCTGAGAA GGGAACTGCC ACCAGAACAA 
GCCGAATACT GTATCAGCCG CATGACCAAG TACATGGGCG GTGATGCTCC TCCAGGCGCG 
CTTGATTATA TCTCCTTCTC AAGTGCTCTG TATGGAGAAA GTGACTTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RNA splicing regulated by RBFOX1 is essential for cardiac function in zebrafish.
Journal of cell science128(16)3030-40(2015 Aug)
Frese KS,Meder B,Keller A,Just S,Haas J,Vogel B,Fischer S,Backes C,Matzas M,K?hler D,Benes V,Katus HA,Rottbauer W

New classes of mind bomb-interacting proteins identified from yeast two-hybrid screens.
PloS one9(4)e93394(2014)
Tseng LC,Zhang C,Cheng CM,Xu H,Hsu CH,Jiang YJ

?-Actinin2 is required for the lateral alignment of Z discs and ventricular chamber enlargement during zebrafish cardiogenesis.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology26(10)4230-42(2012 Oct)
Yang J,Xu X

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actn3a cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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