Actn cDNA ORF clone, Drosophila melanogaster(fruit fly)

The following Actn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Actn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OFa00542 NM_001272238.1
Latest version!
Drosophila melanogaster alpha actinin (Actn), transcript variant J, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa00542 NM_001272237.1
Latest version!
Drosophila melanogaster alpha actinin (Actn), transcript variant H, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa00542 NM_058137.6
Latest version!
Drosophila melanogaster alpha actinin (Actn), transcript variant C, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa00542 NM_001272236.1
Latest version!
Drosophila melanogaster alpha actinin (Actn), transcript variant G, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa00543 NM_058136.4
Latest version!
Drosophila melanogaster alpha actinin (Actn), transcript variant A, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa00544 NM_001201604.2
Latest version!
Drosophila melanogaster alpha actinin (Actn), transcript variant I, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa00544 NM_166920.2
Latest version!
Drosophila melanogaster alpha actinin (Actn), transcript variant B, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa00544 NM_001201603.2
Latest version!
Drosophila melanogaster alpha actinin (Actn), transcript variant D, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OFa03775 NM_001272235.1
Latest version!
Drosophila melanogaster alpha actinin (Actn), transcript variant F, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OFa00542
    Clone ID Related Accession (Same CDS sequence) NM_001272236.1 , NM_001272237.1 , NM_001272238.1 , NM_058137.6
    Accession Version NM_001272236.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2754bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product alpha actinin, isoform G
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGATGATGG AGAACGGACT CTCCATGGAG TACGGAGATG GCTACATGGA GCAGGAGGAG 
    GAGTGGGAGC GCGAGGGCCT GCTCGATCCT GCGTGGGAGA AGCAACAGAA GAAGACATTT
    ACCGCCTGGT GTAACAGCCA TTTGCGCAAG GCCGGTACAG CCATCGACAA CATCGAGGAG
    GACTTCCGCA ACGGCCTCAA GCTGATGCTG CTGCTGGAGG TGATCTCCGG CGAGACGCTC
    CCCAAGCCCG ATCGCGGCAA GATGCGCTTC CACAAGATCG CCAACGTGAA CAAGGCTCTC
    GACTTCATCG CCTCCAAGGG CGTCCACCTG GTCTCTATCG GCGCCGAGGA GATCGTCGAT
    GGAAACTTGA AGATGACTCT GGGTATGATC TGGACGATTA TCCTGCGCTT CGCCATTCAG
    GACATCTCCG TGGAGGAGAT GACCGCCAAG GAGGGTCTGC TGCTGTGGTG CCAGCGCAAG
    ACGGCTCCCT ACAAGAACGT CAACGTACAG AACTTCCATC TGTCGTTCAA GGATGGTCTG
    GCCTTCTGCG CCCTTATCCA TCGCCATCGT CCAGATCTAA TCGACTACGC CAAGCTGTCC
    AAAGACAATC CTCTGGAGAA TCTTAATACT GCCTTCGATG TGGCCGAGAA GTACCTGGAT
    ATTCCGCGCA TGTTGGATCC AGATGATTTG ATCAACACTC CGAAACCGGA TGAACGTGCC
    ATCATGACGT ACGTCTCCTG CTACTACCAC GCCTTCCAGG GAGCTCAACA GGTTGGAAAT
    GTGACGCATG TACCCGAACC CACAAGACAA TACACCTACG TCCCGAATAA TTACAATGCG
    GAAACCGCTG CCAACCGGAT CTGCAAGGTG CTCAAGGTCA ACCAGGAGAA CGAACGCCTC
    ATGGAGGAGT ACGAGCGTCT GGCCAGTGAT TTGCTGGAGT GGATCCGTCG CACCATGCCC
    TGGTTGAACT CGCGCCAGGC CGACAACTCG CTGGCAGGTG TGCAGAAGAA GCTGGAGGAG
    TACCGCACCT ACCGTCGCAA GCACAAGCCA CCACGTGTGG AGCAGAAGGC CAAGCTGGAG
    ACCAACTTCA ACACGCTGCA GACCAAGCTG CGCCTGTCCA ACCGGCCCGC CTACCTGCCC
    ACCGAGGGCA AGACCGTGTC CGACATCTCC AACTCGTGGA AGGGCCTGGA GCTGGCCGAG
    AAGGCCTTTG AGGAATGGCT GCTGGCCGAG ACCATGCGCC TGGAGCGCCT TGAACATCTG
    GCCCAGAAGT TCAAGCACAA GGCCGATGCC CACGAGGACT GGACGCGTGG CAAGGAGGAG
    ATGCTGCAGT CGCAGGACTT CCGCCAGTGC AAGCTCAACG AGCTGAAGGC CCTAAAGAAG
    AAGCACGAAG CCTTTGAGTC TGACCTGGCC GCCCACCAGG ATCGTGTGGA GCAGATTGCC
    GCCATCGCCC AGGAGCTTAA CACTCTGGAG TACCATGACT GTGTGTCTGT GAACGCCAGG
    TGCCAGCGCA TCTGCGACCA ATGGGATCGC TTGGGTGCTC TTACCCAGCG CAGGCGCACC
    GCCTTAGACG AGGCCGAGCG AATCCTGGAG AAGATCGACA TCTTGCATTT GGAGTTCGCG
    AAGCGTGCGG CGCCGTTCAA CAACTGGCTG GATGGTACGC GCGAGGATCT TGTGGACATG
    TTTATTGTAC ACACCATGGA GGAAATCCAA GGCCTGATCC AGGCGCACGA CCAGTTCAAG
    GCGACCCTGG GTGAGGCCGA CAAGGAGTTC AACCTGATCG TGAACCTGGT CCGCGAAGTG
    GAGTCGATTG TGAAGCAGCA CCAGATCCCC GGCGGCCTAG AGAACCCCTA CACCACCCTG
    ACCGCCAACG ACATGACGCG CAAGTGGAGC GACGTCCGCC AACTGGTGCC CCAACGCGAC
    CAGACTTTGG CCAACGAGCT GCGCAAGCAA CAGAACAACG AAATGCTCCG CCGTCAGTTT
    GCCGAGAAGG CCAACATTGT CGGCCCCTGG ATCGAGCGGC AAATGGATGC TGTTACGGCC
    ATCGGTATGG GACTGCAGGG ATCGCTGGAG GACCAACTGC ACCGGCTCAA GGAGTACGAG
    CAGGCCGTCT ACGCCTACAA GCCGAACATT GAGGAGCTGG AGAAGATCCA CCAAGCGGTG
    CAGGAATCGA TGATCTTCGA GAACCGATAC ACCAACTACA CGATGGAAAC GCTGCGCGTC
    GGCTGGGAGC AGCTGCTTAC ATCTATCAAC CGCAACATCA ACGAGGTGGA GAACCAGATT
    CTCACCCGCG ACTCCAAGGG CATTAGCCAG GAGCAGCTGA ACGAATTCCG CAGCAGCTTC
    AACCACTTCG ACAAGAACCG CACCGGCCGA CTGTCGCCCG AGGAGTTCAA GTCGTGCTTG
    GTCTCGTTGG GCTACTCGAT TGGCAAGGAT CGCCAGGGTG ATTTGGACTT CCAGCGCATC
    CTCGCCGTCG TCGATCCCAA CAACACTGGC TACGTGCACT TCGACGCCTT CCTCGACTTC
    ATGACCCGCG AGAGCACCGA TACCGACACT GCCGAACAAG TCATCGACTC CTTCCGAATC
    CTGGCAGCCG ACAAGCCATA CATTTTGCCC GACGAACTGC GTCGCGAGTT GCCGCCAGAC
    CAGGCCGAGT ACTGCATCCA GCGCATGCCG CCCTATAAGG GACCCAACGG AGTGCCCGGC
    GCCCTGGACT ACATGTCCTT CAGCACAGCT CTCTACGGCG AGACCGACTT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001259165.1
    CDS191..2944
    Translation

    Target ORF information:

    RefSeq Version NM_001272236.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster alpha actinin (Actn), transcript variant G, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001272236.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGATGATGG AGAACGGACT CTCCATGGAG TACGGAGATG GCTACATGGA GCAGGAGGAG 
    GAGTGGGAGC GCGAGGGCCT GCTCGATCCT GCGTGGGAGA AGCAACAGAA GAAGACATTT
    ACCGCCTGGT GTAACAGCCA TTTGCGCAAG GCCGGTACAG CCATCGACAA CATCGAGGAG
    GACTTCCGCA ACGGCCTCAA GCTGATGCTG CTGCTGGAGG TGATCTCCGG CGAGACGCTC
    CCCAAGCCCG ATCGCGGCAA GATGCGCTTC CACAAGATCG CCAACGTGAA CAAGGCTCTC
    GACTTCATCG CCTCCAAGGG CGTCCACCTG GTCTCTATCG GCGCCGAGGA GATCGTCGAT
    GGAAACTTGA AGATGACTCT GGGTATGATC TGGACGATTA TCCTGCGCTT CGCCATTCAG
    GACATCTCCG TGGAGGAGAT GACCGCCAAG GAGGGTCTGC TGCTGTGGTG CCAGCGCAAG
    ACGGCTCCCT ACAAGAACGT CAACGTACAG AACTTCCATC TGTCGTTCAA GGATGGTCTG
    GCCTTCTGCG CCCTTATCCA TCGCCATCGT CCAGATCTAA TCGACTACGC CAAGCTGTCC
    AAAGACAATC CTCTGGAGAA TCTTAATACT GCCTTCGATG TGGCCGAGAA GTACCTGGAT
    ATTCCGCGCA TGTTGGATCC AGATGATTTG ATCAACACTC CGAAACCGGA TGAACGTGCC
    ATCATGACGT ACGTCTCCTG CTACTACCAC GCCTTCCAGG GAGCTCAACA GGTTGGAAAT
    GTGACGCATG TACCCGAACC CACAAGACAA TACACCTACG TCCCGAATAA TTACAATGCG
    GAAACCGCTG CCAACCGGAT CTGCAAGGTG CTCAAGGTCA ACCAGGAGAA CGAACGCCTC
    ATGGAGGAGT ACGAGCGTCT GGCCAGTGAT TTGCTGGAGT GGATCCGTCG CACCATGCCC
    TGGTTGAACT CGCGCCAGGC CGACAACTCG CTGGCAGGTG TGCAGAAGAA GCTGGAGGAG
    TACCGCACCT ACCGTCGCAA GCACAAGCCA CCACGTGTGG AGCAGAAGGC CAAGCTGGAG
    ACCAACTTCA ACACGCTGCA GACCAAGCTG CGCCTGTCCA ACCGGCCCGC CTACCTGCCC
    ACCGAGGGCA AGACCGTGTC CGACATCTCC AACTCGTGGA AGGGCCTGGA GCTGGCCGAG
    AAGGCCTTTG AGGAATGGCT GCTGGCCGAG ACCATGCGCC TGGAGCGCCT TGAACATCTG
    GCCCAGAAGT TCAAGCACAA GGCCGATGCC CACGAGGACT GGACGCGTGG CAAGGAGGAG
    ATGCTGCAGT CGCAGGACTT CCGCCAGTGC AAGCTCAACG AGCTGAAGGC CCTAAAGAAG
    AAGCACGAAG CCTTTGAGTC TGACCTGGCC GCCCACCAGG ATCGTGTGGA GCAGATTGCC
    GCCATCGCCC AGGAGCTTAA CACTCTGGAG TACCATGACT GTGTGTCTGT GAACGCCAGG
    TGCCAGCGCA TCTGCGACCA ATGGGATCGC TTGGGTGCTC TTACCCAGCG CAGGCGCACC
    GCCTTAGACG AGGCCGAGCG AATCCTGGAG AAGATCGACA TCTTGCATTT GGAGTTCGCG
    AAGCGTGCGG CGCCGTTCAA CAACTGGCTG GATGGTACGC GCGAGGATCT TGTGGACATG
    TTTATTGTAC ACACCATGGA GGAAATCCAA GGCCTGATCC AGGCGCACGA CCAGTTCAAG
    GCGACCCTGG GTGAGGCCGA CAAGGAGTTC AACCTGATCG TGAACCTGGT CCGCGAAGTG
    GAGTCGATTG TGAAGCAGCA CCAGATCCCC GGCGGCCTAG AGAACCCCTA CACCACCCTG
    ACCGCCAACG ACATGACGCG CAAGTGGAGC GACGTCCGCC AACTGGTGCC CCAACGCGAC
    CAGACTTTGG CCAACGAGCT GCGCAAGCAA CAGAACAACG AAATGCTCCG CCGTCAGTTT
    GCCGAGAAGG CCAACATTGT CGGCCCCTGG ATCGAGCGGC AAATGGATGC TGTTACGGCC
    ATCGGTATGG GACTGCAGGG ATCGCTGGAG GACCAACTGC ACCGGCTCAA GGAGTACGAG
    CAGGCCGTCT ACGCCTACAA GCCGAACATT GAGGAGCTGG AGAAGATCCA CCAAGCGGTG
    CAGGAATCGA TGATCTTCGA GAACCGATAC ACCAACTACA CGATGGAAAC GCTGCGCGTC
    GGCTGGGAGC AGCTGCTTAC ATCTATCAAC CGCAACATCA ACGAGGTGGA GAACCAGATT
    CTCACCCGCG ACTCCAAGGG CATTAGCCAG GAGCAGCTGA ACGAATTCCG CAGCAGCTTC
    AACCACTTCG ACAAGAACCG CACCGGCCGA CTGTCGCCCG AGGAGTTCAA GTCGTGCTTG
    GTCTCGTTGG GCTACTCGAT TGGCAAGGAT CGCCAGGGTG ATTTGGACTT CCAGCGCATC
    CTCGCCGTCG TCGATCCCAA CAACACTGGC TACGTGCACT TCGACGCCTT CCTCGACTTC
    ATGACCCGCG AGAGCACCGA TACCGACACT GCCGAACAAG TCATCGACTC CTTCCGAATC
    CTGGCAGCCG ACAAGCCATA CATTTTGCCC GACGAACTGC GTCGCGAGTT GCCGCCAGAC
    CAGGCCGAGT ACTGCATCCA GCGCATGCCG CCCTATAAGG GACCCAACGG AGTGCCCGGC
    GCCCTGGACT ACATGTCCTT CAGCACAGCT CTCTACGGCG AGACCGACTT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa00542
    Clone ID Related Accession (Same CDS sequence) NM_001272236.1 , NM_001272237.1 , NM_001272238.1 , NM_058137.6
    Accession Version NM_001272237.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2754bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product alpha actinin, isoform H
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGATGATGG AGAACGGACT CTCCATGGAG TACGGAGATG GCTACATGGA GCAGGAGGAG 
    GAGTGGGAGC GCGAGGGCCT GCTCGATCCT GCGTGGGAGA AGCAACAGAA GAAGACATTT
    ACCGCCTGGT GTAACAGCCA TTTGCGCAAG GCCGGTACAG CCATCGACAA CATCGAGGAG
    GACTTCCGCA ACGGCCTCAA GCTGATGCTG CTGCTGGAGG TGATCTCCGG CGAGACGCTC
    CCCAAGCCCG ATCGCGGCAA GATGCGCTTC CACAAGATCG CCAACGTGAA CAAGGCTCTC
    GACTTCATCG CCTCCAAGGG CGTCCACCTG GTCTCTATCG GCGCCGAGGA GATCGTCGAT
    GGAAACTTGA AGATGACTCT GGGTATGATC TGGACGATTA TCCTGCGCTT CGCCATTCAG
    GACATCTCCG TGGAGGAGAT GACCGCCAAG GAGGGTCTGC TGCTGTGGTG CCAGCGCAAG
    ACGGCTCCCT ACAAGAACGT CAACGTACAG AACTTCCATC TGTCGTTCAA GGATGGTCTG
    GCCTTCTGCG CCCTTATCCA TCGCCATCGT CCAGATCTAA TCGACTACGC CAAGCTGTCC
    AAAGACAATC CTCTGGAGAA TCTTAATACT GCCTTCGATG TGGCCGAGAA GTACCTGGAT
    ATTCCGCGCA TGTTGGATCC AGATGATTTG ATCAACACTC CGAAACCGGA TGAACGTGCC
    ATCATGACGT ACGTCTCCTG CTACTACCAC GCCTTCCAGG GAGCTCAACA GGTTGGAAAT
    GTGACGCATG TACCCGAACC CACAAGACAA TACACCTACG TCCCGAATAA TTACAATGCG
    GAAACCGCTG CCAACCGGAT CTGCAAGGTG CTCAAGGTCA ACCAGGAGAA CGAACGCCTC
    ATGGAGGAGT ACGAGCGTCT GGCCAGTGAT TTGCTGGAGT GGATCCGTCG CACCATGCCC
    TGGTTGAACT CGCGCCAGGC CGACAACTCG CTGGCAGGTG TGCAGAAGAA GCTGGAGGAG
    TACCGCACCT ACCGTCGCAA GCACAAGCCA CCACGTGTGG AGCAGAAGGC CAAGCTGGAG
    ACCAACTTCA ACACGCTGCA GACCAAGCTG CGCCTGTCCA ACCGGCCCGC CTACCTGCCC
    ACCGAGGGCA AGACCGTGTC CGACATCTCC AACTCGTGGA AGGGCCTGGA GCTGGCCGAG
    AAGGCCTTTG AGGAATGGCT GCTGGCCGAG ACCATGCGCC TGGAGCGCCT TGAACATCTG
    GCCCAGAAGT TCAAGCACAA GGCCGATGCC CACGAGGACT GGACGCGTGG CAAGGAGGAG
    ATGCTGCAGT CGCAGGACTT CCGCCAGTGC AAGCTCAACG AGCTGAAGGC CCTAAAGAAG
    AAGCACGAAG CCTTTGAGTC TGACCTGGCC GCCCACCAGG ATCGTGTGGA GCAGATTGCC
    GCCATCGCCC AGGAGCTTAA CACTCTGGAG TACCATGACT GTGTGTCTGT GAACGCCAGG
    TGCCAGCGCA TCTGCGACCA ATGGGATCGC TTGGGTGCTC TTACCCAGCG CAGGCGCACC
    GCCTTAGACG AGGCCGAGCG AATCCTGGAG AAGATCGACA TCTTGCATTT GGAGTTCGCG
    AAGCGTGCGG CGCCGTTCAA CAACTGGCTG GATGGTACGC GCGAGGATCT TGTGGACATG
    TTTATTGTAC ACACCATGGA GGAAATCCAA GGCCTGATCC AGGCGCACGA CCAGTTCAAG
    GCGACCCTGG GTGAGGCCGA CAAGGAGTTC AACCTGATCG TGAACCTGGT CCGCGAAGTG
    GAGTCGATTG TGAAGCAGCA CCAGATCCCC GGCGGCCTAG AGAACCCCTA CACCACCCTG
    ACCGCCAACG ACATGACGCG CAAGTGGAGC GACGTCCGCC AACTGGTGCC CCAACGCGAC
    CAGACTTTGG CCAACGAGCT GCGCAAGCAA CAGAACAACG AAATGCTCCG CCGTCAGTTT
    GCCGAGAAGG CCAACATTGT CGGCCCCTGG ATCGAGCGGC AAATGGATGC TGTTACGGCC
    ATCGGTATGG GACTGCAGGG ATCGCTGGAG GACCAACTGC ACCGGCTCAA GGAGTACGAG
    CAGGCCGTCT ACGCCTACAA GCCGAACATT GAGGAGCTGG AGAAGATCCA CCAAGCGGTG
    CAGGAATCGA TGATCTTCGA GAACCGATAC ACCAACTACA CGATGGAAAC GCTGCGCGTC
    GGCTGGGAGC AGCTGCTTAC ATCTATCAAC CGCAACATCA ACGAGGTGGA GAACCAGATT
    CTCACCCGCG ACTCCAAGGG CATTAGCCAG GAGCAGCTGA ACGAATTCCG CAGCAGCTTC
    AACCACTTCG ACAAGAACCG CACCGGCCGA CTGTCGCCCG AGGAGTTCAA GTCGTGCTTG
    GTCTCGTTGG GCTACTCGAT TGGCAAGGAT CGCCAGGGTG ATTTGGACTT CCAGCGCATC
    CTCGCCGTCG TCGATCCCAA CAACACTGGC TACGTGCACT TCGACGCCTT CCTCGACTTC
    ATGACCCGCG AGAGCACCGA TACCGACACT GCCGAACAAG TCATCGACTC CTTCCGAATC
    CTGGCAGCCG ACAAGCCATA CATTTTGCCC GACGAACTGC GTCGCGAGTT GCCGCCAGAC
    CAGGCCGAGT ACTGCATCCA GCGCATGCCG CCCTATAAGG GACCCAACGG AGTGCCCGGC
    GCCCTGGACT ACATGTCCTT CAGCACAGCT CTCTACGGCG AGACCGACTT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001259166.1
    CDS173..2926
    Translation

    Target ORF information:

    RefSeq Version NM_001272237.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster alpha actinin (Actn), transcript variant H, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001272237.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGATGATGG AGAACGGACT CTCCATGGAG TACGGAGATG GCTACATGGA GCAGGAGGAG 
    GAGTGGGAGC GCGAGGGCCT GCTCGATCCT GCGTGGGAGA AGCAACAGAA GAAGACATTT
    ACCGCCTGGT GTAACAGCCA TTTGCGCAAG GCCGGTACAG CCATCGACAA CATCGAGGAG
    GACTTCCGCA ACGGCCTCAA GCTGATGCTG CTGCTGGAGG TGATCTCCGG CGAGACGCTC
    CCCAAGCCCG ATCGCGGCAA GATGCGCTTC CACAAGATCG CCAACGTGAA CAAGGCTCTC
    GACTTCATCG CCTCCAAGGG CGTCCACCTG GTCTCTATCG GCGCCGAGGA GATCGTCGAT
    GGAAACTTGA AGATGACTCT GGGTATGATC TGGACGATTA TCCTGCGCTT CGCCATTCAG
    GACATCTCCG TGGAGGAGAT GACCGCCAAG GAGGGTCTGC TGCTGTGGTG CCAGCGCAAG
    ACGGCTCCCT ACAAGAACGT CAACGTACAG AACTTCCATC TGTCGTTCAA GGATGGTCTG
    GCCTTCTGCG CCCTTATCCA TCGCCATCGT CCAGATCTAA TCGACTACGC CAAGCTGTCC
    AAAGACAATC CTCTGGAGAA TCTTAATACT GCCTTCGATG TGGCCGAGAA GTACCTGGAT
    ATTCCGCGCA TGTTGGATCC AGATGATTTG ATCAACACTC CGAAACCGGA TGAACGTGCC
    ATCATGACGT ACGTCTCCTG CTACTACCAC GCCTTCCAGG GAGCTCAACA GGTTGGAAAT
    GTGACGCATG TACCCGAACC CACAAGACAA TACACCTACG TCCCGAATAA TTACAATGCG
    GAAACCGCTG CCAACCGGAT CTGCAAGGTG CTCAAGGTCA ACCAGGAGAA CGAACGCCTC
    ATGGAGGAGT ACGAGCGTCT GGCCAGTGAT TTGCTGGAGT GGATCCGTCG CACCATGCCC
    TGGTTGAACT CGCGCCAGGC CGACAACTCG CTGGCAGGTG TGCAGAAGAA GCTGGAGGAG
    TACCGCACCT ACCGTCGCAA GCACAAGCCA CCACGTGTGG AGCAGAAGGC CAAGCTGGAG
    ACCAACTTCA ACACGCTGCA GACCAAGCTG CGCCTGTCCA ACCGGCCCGC CTACCTGCCC
    ACCGAGGGCA AGACCGTGTC CGACATCTCC AACTCGTGGA AGGGCCTGGA GCTGGCCGAG
    AAGGCCTTTG AGGAATGGCT GCTGGCCGAG ACCATGCGCC TGGAGCGCCT TGAACATCTG
    GCCCAGAAGT TCAAGCACAA GGCCGATGCC CACGAGGACT GGACGCGTGG CAAGGAGGAG
    ATGCTGCAGT CGCAGGACTT CCGCCAGTGC AAGCTCAACG AGCTGAAGGC CCTAAAGAAG
    AAGCACGAAG CCTTTGAGTC TGACCTGGCC GCCCACCAGG ATCGTGTGGA GCAGATTGCC
    GCCATCGCCC AGGAGCTTAA CACTCTGGAG TACCATGACT GTGTGTCTGT GAACGCCAGG
    TGCCAGCGCA TCTGCGACCA ATGGGATCGC TTGGGTGCTC TTACCCAGCG CAGGCGCACC
    GCCTTAGACG AGGCCGAGCG AATCCTGGAG AAGATCGACA TCTTGCATTT GGAGTTCGCG
    AAGCGTGCGG CGCCGTTCAA CAACTGGCTG GATGGTACGC GCGAGGATCT TGTGGACATG
    TTTATTGTAC ACACCATGGA GGAAATCCAA GGCCTGATCC AGGCGCACGA CCAGTTCAAG
    GCGACCCTGG GTGAGGCCGA CAAGGAGTTC AACCTGATCG TGAACCTGGT CCGCGAAGTG
    GAGTCGATTG TGAAGCAGCA CCAGATCCCC GGCGGCCTAG AGAACCCCTA CACCACCCTG
    ACCGCCAACG ACATGACGCG CAAGTGGAGC GACGTCCGCC AACTGGTGCC CCAACGCGAC
    CAGACTTTGG CCAACGAGCT GCGCAAGCAA CAGAACAACG AAATGCTCCG CCGTCAGTTT
    GCCGAGAAGG CCAACATTGT CGGCCCCTGG ATCGAGCGGC AAATGGATGC TGTTACGGCC
    ATCGGTATGG GACTGCAGGG ATCGCTGGAG GACCAACTGC ACCGGCTCAA GGAGTACGAG
    CAGGCCGTCT ACGCCTACAA GCCGAACATT GAGGAGCTGG AGAAGATCCA CCAAGCGGTG
    CAGGAATCGA TGATCTTCGA GAACCGATAC ACCAACTACA CGATGGAAAC GCTGCGCGTC
    GGCTGGGAGC AGCTGCTTAC ATCTATCAAC CGCAACATCA ACGAGGTGGA GAACCAGATT
    CTCACCCGCG ACTCCAAGGG CATTAGCCAG GAGCAGCTGA ACGAATTCCG CAGCAGCTTC
    AACCACTTCG ACAAGAACCG CACCGGCCGA CTGTCGCCCG AGGAGTTCAA GTCGTGCTTG
    GTCTCGTTGG GCTACTCGAT TGGCAAGGAT CGCCAGGGTG ATTTGGACTT CCAGCGCATC
    CTCGCCGTCG TCGATCCCAA CAACACTGGC TACGTGCACT TCGACGCCTT CCTCGACTTC
    ATGACCCGCG AGAGCACCGA TACCGACACT GCCGAACAAG TCATCGACTC CTTCCGAATC
    CTGGCAGCCG ACAAGCCATA CATTTTGCCC GACGAACTGC GTCGCGAGTT GCCGCCAGAC
    CAGGCCGAGT ACTGCATCCA GCGCATGCCG CCCTATAAGG GACCCAACGG AGTGCCCGGC
    GCCCTGGACT ACATGTCCTT CAGCACAGCT CTCTACGGCG AGACCGACTT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa00542
    Clone ID Related Accession (Same CDS sequence) NM_001272236.1 , NM_001272237.1 , NM_001272238.1 , NM_058137.6
    Accession Version NM_001272238.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2754bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product alpha actinin, isoform J
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGATGATGG AGAACGGACT CTCCATGGAG TACGGAGATG GCTACATGGA GCAGGAGGAG 
    GAGTGGGAGC GCGAGGGCCT GCTCGATCCT GCGTGGGAGA AGCAACAGAA GAAGACATTT
    ACCGCCTGGT GTAACAGCCA TTTGCGCAAG GCCGGTACAG CCATCGACAA CATCGAGGAG
    GACTTCCGCA ACGGCCTCAA GCTGATGCTG CTGCTGGAGG TGATCTCCGG CGAGACGCTC
    CCCAAGCCCG ATCGCGGCAA GATGCGCTTC CACAAGATCG CCAACGTGAA CAAGGCTCTC
    GACTTCATCG CCTCCAAGGG CGTCCACCTG GTCTCTATCG GCGCCGAGGA GATCGTCGAT
    GGAAACTTGA AGATGACTCT GGGTATGATC TGGACGATTA TCCTGCGCTT CGCCATTCAG
    GACATCTCCG TGGAGGAGAT GACCGCCAAG GAGGGTCTGC TGCTGTGGTG CCAGCGCAAG
    ACGGCTCCCT ACAAGAACGT CAACGTACAG AACTTCCATC TGTCGTTCAA GGATGGTCTG
    GCCTTCTGCG CCCTTATCCA TCGCCATCGT CCAGATCTAA TCGACTACGC CAAGCTGTCC
    AAAGACAATC CTCTGGAGAA TCTTAATACT GCCTTCGATG TGGCCGAGAA GTACCTGGAT
    ATTCCGCGCA TGTTGGATCC AGATGATTTG ATCAACACTC CGAAACCGGA TGAACGTGCC
    ATCATGACGT ACGTCTCCTG CTACTACCAC GCCTTCCAGG GAGCTCAACA GGTTGGAAAT
    GTGACGCATG TACCCGAACC CACAAGACAA TACACCTACG TCCCGAATAA TTACAATGCG
    GAAACCGCTG CCAACCGGAT CTGCAAGGTG CTCAAGGTCA ACCAGGAGAA CGAACGCCTC
    ATGGAGGAGT ACGAGCGTCT GGCCAGTGAT TTGCTGGAGT GGATCCGTCG CACCATGCCC
    TGGTTGAACT CGCGCCAGGC CGACAACTCG CTGGCAGGTG TGCAGAAGAA GCTGGAGGAG
    TACCGCACCT ACCGTCGCAA GCACAAGCCA CCACGTGTGG AGCAGAAGGC CAAGCTGGAG
    ACCAACTTCA ACACGCTGCA GACCAAGCTG CGCCTGTCCA ACCGGCCCGC CTACCTGCCC
    ACCGAGGGCA AGACCGTGTC CGACATCTCC AACTCGTGGA AGGGCCTGGA GCTGGCCGAG
    AAGGCCTTTG AGGAATGGCT GCTGGCCGAG ACCATGCGCC TGGAGCGCCT TGAACATCTG
    GCCCAGAAGT TCAAGCACAA GGCCGATGCC CACGAGGACT GGACGCGTGG CAAGGAGGAG
    ATGCTGCAGT CGCAGGACTT CCGCCAGTGC AAGCTCAACG AGCTGAAGGC CCTAAAGAAG
    AAGCACGAAG CCTTTGAGTC TGACCTGGCC GCCCACCAGG ATCGTGTGGA GCAGATTGCC
    GCCATCGCCC AGGAGCTTAA CACTCTGGAG TACCATGACT GTGTGTCTGT GAACGCCAGG
    TGCCAGCGCA TCTGCGACCA ATGGGATCGC TTGGGTGCTC TTACCCAGCG CAGGCGCACC
    GCCTTAGACG AGGCCGAGCG AATCCTGGAG AAGATCGACA TCTTGCATTT GGAGTTCGCG
    AAGCGTGCGG CGCCGTTCAA CAACTGGCTG GATGGTACGC GCGAGGATCT TGTGGACATG
    TTTATTGTAC ACACCATGGA GGAAATCCAA GGCCTGATCC AGGCGCACGA CCAGTTCAAG
    GCGACCCTGG GTGAGGCCGA CAAGGAGTTC AACCTGATCG TGAACCTGGT CCGCGAAGTG
    GAGTCGATTG TGAAGCAGCA CCAGATCCCC GGCGGCCTAG AGAACCCCTA CACCACCCTG
    ACCGCCAACG ACATGACGCG CAAGTGGAGC GACGTCCGCC AACTGGTGCC CCAACGCGAC
    CAGACTTTGG CCAACGAGCT GCGCAAGCAA CAGAACAACG AAATGCTCCG CCGTCAGTTT
    GCCGAGAAGG CCAACATTGT CGGCCCCTGG ATCGAGCGGC AAATGGATGC TGTTACGGCC
    ATCGGTATGG GACTGCAGGG ATCGCTGGAG GACCAACTGC ACCGGCTCAA GGAGTACGAG
    CAGGCCGTCT ACGCCTACAA GCCGAACATT GAGGAGCTGG AGAAGATCCA CCAAGCGGTG
    CAGGAATCGA TGATCTTCGA GAACCGATAC ACCAACTACA CGATGGAAAC GCTGCGCGTC
    GGCTGGGAGC AGCTGCTTAC ATCTATCAAC CGCAACATCA ACGAGGTGGA GAACCAGATT
    CTCACCCGCG ACTCCAAGGG CATTAGCCAG GAGCAGCTGA ACGAATTCCG CAGCAGCTTC
    AACCACTTCG ACAAGAACCG CACCGGCCGA CTGTCGCCCG AGGAGTTCAA GTCGTGCTTG
    GTCTCGTTGG GCTACTCGAT TGGCAAGGAT CGCCAGGGTG ATTTGGACTT CCAGCGCATC
    CTCGCCGTCG TCGATCCCAA CAACACTGGC TACGTGCACT TCGACGCCTT CCTCGACTTC
    ATGACCCGCG AGAGCACCGA TACCGACACT GCCGAACAAG TCATCGACTC CTTCCGAATC
    CTGGCAGCCG ACAAGCCATA CATTTTGCCC GACGAACTGC GTCGCGAGTT GCCGCCAGAC
    CAGGCCGAGT ACTGCATCCA GCGCATGCCG CCCTATAAGG GACCCAACGG AGTGCCCGGC
    GCCCTGGACT ACATGTCCTT CAGCACAGCT CTCTACGGCG AGACCGACTT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001259167.1
    CDS188..2941
    Translation

    Target ORF information:

    RefSeq Version NM_001272238.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster alpha actinin (Actn), transcript variant J, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001272238.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGATGATGG AGAACGGACT CTCCATGGAG TACGGAGATG GCTACATGGA GCAGGAGGAG 
    GAGTGGGAGC GCGAGGGCCT GCTCGATCCT GCGTGGGAGA AGCAACAGAA GAAGACATTT
    ACCGCCTGGT GTAACAGCCA TTTGCGCAAG GCCGGTACAG CCATCGACAA CATCGAGGAG
    GACTTCCGCA ACGGCCTCAA GCTGATGCTG CTGCTGGAGG TGATCTCCGG CGAGACGCTC
    CCCAAGCCCG ATCGCGGCAA GATGCGCTTC CACAAGATCG CCAACGTGAA CAAGGCTCTC
    GACTTCATCG CCTCCAAGGG CGTCCACCTG GTCTCTATCG GCGCCGAGGA GATCGTCGAT
    GGAAACTTGA AGATGACTCT GGGTATGATC TGGACGATTA TCCTGCGCTT CGCCATTCAG
    GACATCTCCG TGGAGGAGAT GACCGCCAAG GAGGGTCTGC TGCTGTGGTG CCAGCGCAAG
    ACGGCTCCCT ACAAGAACGT CAACGTACAG AACTTCCATC TGTCGTTCAA GGATGGTCTG
    GCCTTCTGCG CCCTTATCCA TCGCCATCGT CCAGATCTAA TCGACTACGC CAAGCTGTCC
    AAAGACAATC CTCTGGAGAA TCTTAATACT GCCTTCGATG TGGCCGAGAA GTACCTGGAT
    ATTCCGCGCA TGTTGGATCC AGATGATTTG ATCAACACTC CGAAACCGGA TGAACGTGCC
    ATCATGACGT ACGTCTCCTG CTACTACCAC GCCTTCCAGG GAGCTCAACA GGTTGGAAAT
    GTGACGCATG TACCCGAACC CACAAGACAA TACACCTACG TCCCGAATAA TTACAATGCG
    GAAACCGCTG CCAACCGGAT CTGCAAGGTG CTCAAGGTCA ACCAGGAGAA CGAACGCCTC
    ATGGAGGAGT ACGAGCGTCT GGCCAGTGAT TTGCTGGAGT GGATCCGTCG CACCATGCCC
    TGGTTGAACT CGCGCCAGGC CGACAACTCG CTGGCAGGTG TGCAGAAGAA GCTGGAGGAG
    TACCGCACCT ACCGTCGCAA GCACAAGCCA CCACGTGTGG AGCAGAAGGC CAAGCTGGAG
    ACCAACTTCA ACACGCTGCA GACCAAGCTG CGCCTGTCCA ACCGGCCCGC CTACCTGCCC
    ACCGAGGGCA AGACCGTGTC CGACATCTCC AACTCGTGGA AGGGCCTGGA GCTGGCCGAG
    AAGGCCTTTG AGGAATGGCT GCTGGCCGAG ACCATGCGCC TGGAGCGCCT TGAACATCTG
    GCCCAGAAGT TCAAGCACAA GGCCGATGCC CACGAGGACT GGACGCGTGG CAAGGAGGAG
    ATGCTGCAGT CGCAGGACTT CCGCCAGTGC AAGCTCAACG AGCTGAAGGC CCTAAAGAAG
    AAGCACGAAG CCTTTGAGTC TGACCTGGCC GCCCACCAGG ATCGTGTGGA GCAGATTGCC
    GCCATCGCCC AGGAGCTTAA CACTCTGGAG TACCATGACT GTGTGTCTGT GAACGCCAGG
    TGCCAGCGCA TCTGCGACCA ATGGGATCGC TTGGGTGCTC TTACCCAGCG CAGGCGCACC
    GCCTTAGACG AGGCCGAGCG AATCCTGGAG AAGATCGACA TCTTGCATTT GGAGTTCGCG
    AAGCGTGCGG CGCCGTTCAA CAACTGGCTG GATGGTACGC GCGAGGATCT TGTGGACATG
    TTTATTGTAC ACACCATGGA GGAAATCCAA GGCCTGATCC AGGCGCACGA CCAGTTCAAG
    GCGACCCTGG GTGAGGCCGA CAAGGAGTTC AACCTGATCG TGAACCTGGT CCGCGAAGTG
    GAGTCGATTG TGAAGCAGCA CCAGATCCCC GGCGGCCTAG AGAACCCCTA CACCACCCTG
    ACCGCCAACG ACATGACGCG CAAGTGGAGC GACGTCCGCC AACTGGTGCC CCAACGCGAC
    CAGACTTTGG CCAACGAGCT GCGCAAGCAA CAGAACAACG AAATGCTCCG CCGTCAGTTT
    GCCGAGAAGG CCAACATTGT CGGCCCCTGG ATCGAGCGGC AAATGGATGC TGTTACGGCC
    ATCGGTATGG GACTGCAGGG ATCGCTGGAG GACCAACTGC ACCGGCTCAA GGAGTACGAG
    CAGGCCGTCT ACGCCTACAA GCCGAACATT GAGGAGCTGG AGAAGATCCA CCAAGCGGTG
    CAGGAATCGA TGATCTTCGA GAACCGATAC ACCAACTACA CGATGGAAAC GCTGCGCGTC
    GGCTGGGAGC AGCTGCTTAC ATCTATCAAC CGCAACATCA ACGAGGTGGA GAACCAGATT
    CTCACCCGCG ACTCCAAGGG CATTAGCCAG GAGCAGCTGA ACGAATTCCG CAGCAGCTTC
    AACCACTTCG ACAAGAACCG CACCGGCCGA CTGTCGCCCG AGGAGTTCAA GTCGTGCTTG
    GTCTCGTTGG GCTACTCGAT TGGCAAGGAT CGCCAGGGTG ATTTGGACTT CCAGCGCATC
    CTCGCCGTCG TCGATCCCAA CAACACTGGC TACGTGCACT TCGACGCCTT CCTCGACTTC
    ATGACCCGCG AGAGCACCGA TACCGACACT GCCGAACAAG TCATCGACTC CTTCCGAATC
    CTGGCAGCCG ACAAGCCATA CATTTTGCCC GACGAACTGC GTCGCGAGTT GCCGCCAGAC
    CAGGCCGAGT ACTGCATCCA GCGCATGCCG CCCTATAAGG GACCCAACGG AGTGCCCGGC
    GCCCTGGACT ACATGTCCTT CAGCACAGCT CTCTACGGCG AGACCGACTT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa00542
    Clone ID Related Accession (Same CDS sequence) NM_001272236.1 , NM_001272237.1 , NM_001272238.1 , NM_058137.6
    Accession Version NM_058137.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2754bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product alpha actinin, isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_058137.5. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGATGATGG AGAACGGACT CTCCATGGAG TACGGAGATG GCTACATGGA GCAGGAGGAG 
    GAGTGGGAGC GCGAGGGCCT GCTCGATCCT GCGTGGGAGA AGCAACAGAA GAAGACATTT
    ACCGCCTGGT GTAACAGCCA TTTGCGCAAG GCCGGTACAG CCATCGACAA CATCGAGGAG
    GACTTCCGCA ACGGCCTCAA GCTGATGCTG CTGCTGGAGG TGATCTCCGG CGAGACGCTC
    CCCAAGCCCG ATCGCGGCAA GATGCGCTTC CACAAGATCG CCAACGTGAA CAAGGCTCTC
    GACTTCATCG CCTCCAAGGG CGTCCACCTG GTCTCTATCG GCGCCGAGGA GATCGTCGAT
    GGAAACTTGA AGATGACTCT GGGTATGATC TGGACGATTA TCCTGCGCTT CGCCATTCAG
    GACATCTCCG TGGAGGAGAT GACCGCCAAG GAGGGTCTGC TGCTGTGGTG CCAGCGCAAG
    ACGGCTCCCT ACAAGAACGT CAACGTACAG AACTTCCATC TGTCGTTCAA GGATGGTCTG
    GCCTTCTGCG CCCTTATCCA TCGCCATCGT CCAGATCTAA TCGACTACGC CAAGCTGTCC
    AAAGACAATC CTCTGGAGAA TCTTAATACT GCCTTCGATG TGGCCGAGAA GTACCTGGAT
    ATTCCGCGCA TGTTGGATCC AGATGATTTG ATCAACACTC CGAAACCGGA TGAACGTGCC
    ATCATGACGT ACGTCTCCTG CTACTACCAC GCCTTCCAGG GAGCTCAACA GGTTGGAAAT
    GTGACGCATG TACCCGAACC CACAAGACAA TACACCTACG TCCCGAATAA TTACAATGCG
    GAAACCGCTG CCAACCGGAT CTGCAAGGTG CTCAAGGTCA ACCAGGAGAA CGAACGCCTC
    ATGGAGGAGT ACGAGCGTCT GGCCAGTGAT TTGCTGGAGT GGATCCGTCG CACCATGCCC
    TGGTTGAACT CGCGCCAGGC CGACAACTCG CTGGCAGGTG TGCAGAAGAA GCTGGAGGAG
    TACCGCACCT ACCGTCGCAA GCACAAGCCA CCACGTGTGG AGCAGAAGGC CAAGCTGGAG
    ACCAACTTCA ACACGCTGCA GACCAAGCTG CGCCTGTCCA ACCGGCCCGC CTACCTGCCC
    ACCGAGGGCA AGACCGTGTC CGACATCTCC AACTCGTGGA AGGGCCTGGA GCTGGCCGAG
    AAGGCCTTTG AGGAATGGCT GCTGGCCGAG ACCATGCGCC TGGAGCGCCT TGAACATCTG
    GCCCAGAAGT TCAAGCACAA GGCCGATGCC CACGAGGACT GGACGCGTGG CAAGGAGGAG
    ATGCTGCAGT CGCAGGACTT CCGCCAGTGC AAGCTCAACG AGCTGAAGGC CCTAAAGAAG
    AAGCACGAAG CCTTTGAGTC TGACCTGGCC GCCCACCAGG ATCGTGTGGA GCAGATTGCC
    GCCATCGCCC AGGAGCTTAA CACTCTGGAG TACCATGACT GTGTGTCTGT GAACGCCAGG
    TGCCAGCGCA TCTGCGACCA ATGGGATCGC TTGGGTGCTC TTACCCAGCG CAGGCGCACC
    GCCTTAGACG AGGCCGAGCG AATCCTGGAG AAGATCGACA TCTTGCATTT GGAGTTCGCG
    AAGCGTGCGG CGCCGTTCAA CAACTGGCTG GATGGTACGC GCGAGGATCT TGTGGACATG
    TTTATTGTAC ACACCATGGA GGAAATCCAA GGCCTGATCC AGGCGCACGA CCAGTTCAAG
    GCGACCCTGG GTGAGGCCGA CAAGGAGTTC AACCTGATCG TGAACCTGGT CCGCGAAGTG
    GAGTCGATTG TGAAGCAGCA CCAGATCCCC GGCGGCCTAG AGAACCCCTA CACCACCCTG
    ACCGCCAACG ACATGACGCG CAAGTGGAGC GACGTCCGCC AACTGGTGCC CCAACGCGAC
    CAGACTTTGG CCAACGAGCT GCGCAAGCAA CAGAACAACG AAATGCTCCG CCGTCAGTTT
    GCCGAGAAGG CCAACATTGT CGGCCCCTGG ATCGAGCGGC AAATGGATGC TGTTACGGCC
    ATCGGTATGG GACTGCAGGG ATCGCTGGAG GACCAACTGC ACCGGCTCAA GGAGTACGAG
    CAGGCCGTCT ACGCCTACAA GCCGAACATT GAGGAGCTGG AGAAGATCCA CCAAGCGGTG
    CAGGAATCGA TGATCTTCGA GAACCGATAC ACCAACTACA CGATGGAAAC GCTGCGCGTC
    GGCTGGGAGC AGCTGCTTAC ATCTATCAAC CGCAACATCA ACGAGGTGGA GAACCAGATT
    CTCACCCGCG ACTCCAAGGG CATTAGCCAG GAGCAGCTGA ACGAATTCCG CAGCAGCTTC
    AACCACTTCG ACAAGAACCG CACCGGCCGA CTGTCGCCCG AGGAGTTCAA GTCGTGCTTG
    GTCTCGTTGG GCTACTCGAT TGGCAAGGAT CGCCAGGGTG ATTTGGACTT CCAGCGCATC
    CTCGCCGTCG TCGATCCCAA CAACACTGGC TACGTGCACT TCGACGCCTT CCTCGACTTC
    ATGACCCGCG AGAGCACCGA TACCGACACT GCCGAACAAG TCATCGACTC CTTCCGAATC
    CTGGCAGCCG ACAAGCCATA CATTTTGCCC GACGAACTGC GTCGCGAGTT GCCGCCAGAC
    CAGGCCGAGT ACTGCATCCA GCGCATGCCG CCCTATAAGG GACCCAACGG AGTGCCCGGC
    GCCCTGGACT ACATGTCCTT CAGCACAGCT CTCTACGGCG AGACCGACTT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_477485.1
    CDS396..3149
    Translation

    Target ORF information:

    RefSeq Version NM_058137.6
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster alpha actinin (Actn), transcript variant C, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_058137.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGATGATGG AGAACGGACT CTCCATGGAG TACGGAGATG GCTACATGGA GCAGGAGGAG 
    GAGTGGGAGC GCGAGGGCCT GCTCGATCCT GCGTGGGAGA AGCAACAGAA GAAGACATTT
    ACCGCCTGGT GTAACAGCCA TTTGCGCAAG GCCGGTACAG CCATCGACAA CATCGAGGAG
    GACTTCCGCA ACGGCCTCAA GCTGATGCTG CTGCTGGAGG TGATCTCCGG CGAGACGCTC
    CCCAAGCCCG ATCGCGGCAA GATGCGCTTC CACAAGATCG CCAACGTGAA CAAGGCTCTC
    GACTTCATCG CCTCCAAGGG CGTCCACCTG GTCTCTATCG GCGCCGAGGA GATCGTCGAT
    GGAAACTTGA AGATGACTCT GGGTATGATC TGGACGATTA TCCTGCGCTT CGCCATTCAG
    GACATCTCCG TGGAGGAGAT GACCGCCAAG GAGGGTCTGC TGCTGTGGTG CCAGCGCAAG
    ACGGCTCCCT ACAAGAACGT CAACGTACAG AACTTCCATC TGTCGTTCAA GGATGGTCTG
    GCCTTCTGCG CCCTTATCCA TCGCCATCGT CCAGATCTAA TCGACTACGC CAAGCTGTCC
    AAAGACAATC CTCTGGAGAA TCTTAATACT GCCTTCGATG TGGCCGAGAA GTACCTGGAT
    ATTCCGCGCA TGTTGGATCC AGATGATTTG ATCAACACTC CGAAACCGGA TGAACGTGCC
    ATCATGACGT ACGTCTCCTG CTACTACCAC GCCTTCCAGG GAGCTCAACA GGTTGGAAAT
    GTGACGCATG TACCCGAACC CACAAGACAA TACACCTACG TCCCGAATAA TTACAATGCG
    GAAACCGCTG CCAACCGGAT CTGCAAGGTG CTCAAGGTCA ACCAGGAGAA CGAACGCCTC
    ATGGAGGAGT ACGAGCGTCT GGCCAGTGAT TTGCTGGAGT GGATCCGTCG CACCATGCCC
    TGGTTGAACT CGCGCCAGGC CGACAACTCG CTGGCAGGTG TGCAGAAGAA GCTGGAGGAG
    TACCGCACCT ACCGTCGCAA GCACAAGCCA CCACGTGTGG AGCAGAAGGC CAAGCTGGAG
    ACCAACTTCA ACACGCTGCA GACCAAGCTG CGCCTGTCCA ACCGGCCCGC CTACCTGCCC
    ACCGAGGGCA AGACCGTGTC CGACATCTCC AACTCGTGGA AGGGCCTGGA GCTGGCCGAG
    AAGGCCTTTG AGGAATGGCT GCTGGCCGAG ACCATGCGCC TGGAGCGCCT TGAACATCTG
    GCCCAGAAGT TCAAGCACAA GGCCGATGCC CACGAGGACT GGACGCGTGG CAAGGAGGAG
    ATGCTGCAGT CGCAGGACTT CCGCCAGTGC AAGCTCAACG AGCTGAAGGC CCTAAAGAAG
    AAGCACGAAG CCTTTGAGTC TGACCTGGCC GCCCACCAGG ATCGTGTGGA GCAGATTGCC
    GCCATCGCCC AGGAGCTTAA CACTCTGGAG TACCATGACT GTGTGTCTGT GAACGCCAGG
    TGCCAGCGCA TCTGCGACCA ATGGGATCGC TTGGGTGCTC TTACCCAGCG CAGGCGCACC
    GCCTTAGACG AGGCCGAGCG AATCCTGGAG AAGATCGACA TCTTGCATTT GGAGTTCGCG
    AAGCGTGCGG CGCCGTTCAA CAACTGGCTG GATGGTACGC GCGAGGATCT TGTGGACATG
    TTTATTGTAC ACACCATGGA GGAAATCCAA GGCCTGATCC AGGCGCACGA CCAGTTCAAG
    GCGACCCTGG GTGAGGCCGA CAAGGAGTTC AACCTGATCG TGAACCTGGT CCGCGAAGTG
    GAGTCGATTG TGAAGCAGCA CCAGATCCCC GGCGGCCTAG AGAACCCCTA CACCACCCTG
    ACCGCCAACG ACATGACGCG CAAGTGGAGC GACGTCCGCC AACTGGTGCC CCAACGCGAC
    CAGACTTTGG CCAACGAGCT GCGCAAGCAA CAGAACAACG AAATGCTCCG CCGTCAGTTT
    GCCGAGAAGG CCAACATTGT CGGCCCCTGG ATCGAGCGGC AAATGGATGC TGTTACGGCC
    ATCGGTATGG GACTGCAGGG ATCGCTGGAG GACCAACTGC ACCGGCTCAA GGAGTACGAG
    CAGGCCGTCT ACGCCTACAA GCCGAACATT GAGGAGCTGG AGAAGATCCA CCAAGCGGTG
    CAGGAATCGA TGATCTTCGA GAACCGATAC ACCAACTACA CGATGGAAAC GCTGCGCGTC
    GGCTGGGAGC AGCTGCTTAC ATCTATCAAC CGCAACATCA ACGAGGTGGA GAACCAGATT
    CTCACCCGCG ACTCCAAGGG CATTAGCCAG GAGCAGCTGA ACGAATTCCG CAGCAGCTTC
    AACCACTTCG ACAAGAACCG CACCGGCCGA CTGTCGCCCG AGGAGTTCAA GTCGTGCTTG
    GTCTCGTTGG GCTACTCGAT TGGCAAGGAT CGCCAGGGTG ATTTGGACTT CCAGCGCATC
    CTCGCCGTCG TCGATCCCAA CAACACTGGC TACGTGCACT TCGACGCCTT CCTCGACTTC
    ATGACCCGCG AGAGCACCGA TACCGACACT GCCGAACAAG TCATCGACTC CTTCCGAATC
    CTGGCAGCCG ACAAGCCATA CATTTTGCCC GACGAACTGC GTCGCGAGTT GCCGCCAGAC
    CAGGCCGAGT ACTGCATCCA GCGCATGCCG CCCTATAAGG GACCCAACGG AGTGCCCGGC
    GCCCTGGACT ACATGTCCTT CAGCACAGCT CTCTACGGCG AGACCGACTT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa00543
    Clone ID Related Accession (Same CDS sequence) NM_058136.4
    Accession Version NM_058136.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2688bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product alpha actinin, isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 31, 2011 this sequence version replaced NM_058136.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGATGATGG AGAACGGACT CTCCATGGAG TACGGAGATG GCTACATGGA GCAGGAGGAG 
    GAGTGGGAGC GCGAGGGCCT GCTCGATCCT GCGTGGGAGA AGCAACAGAA GAAGACATTT
    ACCGCCTGGT GTAACAGCCA TTTGCGCAAG GCCGGTACAG CCATCGACAA CATCGAGGAG
    GACTTCCGCA ACGGCCTCAA GCTGATGCTG CTGCTGGAGG TGATCTCCGG CGAGACGCTC
    CCCAAGCCCG ATCGCGGCAA GATGCGCTTC CACAAGATCG CCAACGTGAA CAAGGCTCTC
    GACTTCATCG CCTCCAAGGG CGTCCACCTG GTCTCTATCG GCGCCGAGGA GATCGTCGAT
    GGAAACTTGA AGATGACTCT GGGTATGATC TGGACGATTA TCCTGCGCTT CGCCATTCAG
    GACATCTCCG TGGAGGAGAT GACCGCCAAG GAGGGTCTGC TGCTGTGGTG CCAGCGCAAG
    ACGGCTCCCT ACAAGAACGT CAACGTACAG AACTTCCATC TGTCGTTCAA GGATGGTCTG
    GCCTTCTGCG CCCTTATCCA TCGCCATCGT CCAGATCTAA TCGACTACGC CAAGCTGTCC
    AAAGACAATC CTCTGGAGAA TCTTAATACT GCCTTCGATG TGGCCGAGAA GTACCTGGAT
    ATTCCGCGCA TGTTGGATCC AGATGATCTC CAGAATACAG CTTTGCCCGA TGAGCGGGCG
    GTGATGACGT ATGTGTCCTC GTACTATCAC TGCTTCAGTG GCGCCCAAAA GGCGGAAACC
    GCTGCCAACC GGATCTGCAA GGTGCTCAAG GTCAACCAGG AGAACGAACG CCTCATGGAG
    GAGTACGAGC GTCTGGCCAG TGATTTGCTG GAGTGGATCC GTCGCACCAT GCCCTGGTTG
    AACTCGCGCC AGGCCGACAA CTCGCTGGCA GGTGTGCAGA AGAAGCTGGA GGAGTACCGC
    ACCTACCGTC GCAAGCACAA GCCACCACGT GTGGAGCAGA AGGCCAAGCT GGAGACCAAC
    TTCAACACGC TGCAGACCAA GCTGCGCCTG TCCAACCGGC CCGCCTACCT GCCCACCGAG
    GGCAAGACCG TGTCCGACAT CTCCAACTCG TGGAAGGGCC TGGAGCTGGC CGAGAAGGCC
    TTTGAGGAAT GGCTGCTGGC CGAGACCATG CGCCTGGAGC GCCTTGAACA TCTGGCCCAG
    AAGTTCAAGC ACAAGGCCGA TGCCCACGAG GACTGGACGC GTGGCAAGGA GGAGATGCTG
    CAGTCGCAGG ACTTCCGCCA GTGCAAGCTC AACGAGCTGA AGGCCCTAAA GAAGAAGCAC
    GAAGCCTTTG AGTCTGACCT GGCCGCCCAC CAGGATCGTG TGGAGCAGAT TGCCGCCATC
    GCCCAGGAGC TTAACACTCT GGAGTACCAT GACTGTGTGT CTGTGAACGC CAGGTGCCAG
    CGCATCTGCG ACCAATGGGA TCGCTTGGGT GCTCTTACCC AGCGCAGGCG CACCGCCTTA
    GACGAGGCCG AGCGAATCCT GGAGAAGATC GACATCTTGC ATTTGGAGTT CGCGAAGCGT
    GCGGCGCCGT TCAACAACTG GCTGGATGGT ACGCGCGAGG ATCTTGTGGA CATGTTTATT
    GTACACACCA TGGAGGAAAT CCAAGGCCTG ATCCAGGCGC ACGACCAGTT CAAGGCGACC
    CTGGGTGAGG CCGACAAGGA GTTCAACCTG ATCGTGAACC TGGTCCGCGA AGTGGAGTCG
    ATTGTGAAGC AGCACCAGAT CCCCGGCGGC CTAGAGAACC CCTACACCAC CCTGACCGCC
    AACGACATGA CGCGCAAGTG GAGCGACGTC CGCCAACTGG TGCCCCAACG CGACCAGACT
    TTGGCCAACG AGCTGCGCAA GCAACAGAAC AACGAAATGC TCCGCCGTCA GTTTGCCGAG
    AAGGCCAACA TTGTCGGCCC CTGGATCGAG CGGCAAATGG ATGCTGTTAC GGCCATCGGT
    ATGGGACTGC AGGGATCGCT GGAGGACCAA CTGCACCGGC TCAAGGAGTA CGAGCAGGCC
    GTCTACGCCT ACAAGCCGAA CATTGAGGAG CTGGAGAAGA TCCACCAAGC GGTGCAGGAA
    TCGATGATCT TCGAGAACCG ATACACCAAC TACACGATGG AAACGCTGCG CGTCGGCTGG
    GAGCAGCTGC TTACATCTAT CAACCGCAAC ATCAACGAGG TGGAGAACCA GATTCTCACC
    CGCGACTCCA AGGGCATTAG CCAGGAGCAG CTGAACGAAT TCCGCAGCAG CTTCAACCAC
    TTCGACAAGA ACCGCACCGG CCGACTGTCG CCCGAGGAGT TCAAGTCGTG CTTGGTCTCG
    TTGGGCTACT CGATTGGCAA GGATCGCCAG GGTGATTTGG ACTTCCAGCG CATCCTCGCC
    GTCGTCGATC CCAACAACAC TGGCTACGTG CACTTCGACG CCTTCCTCGA CTTCATGACC
    CGCGAGAGCA CCGATACCGA CACTGCCGAA CAAGTCATCG ACTCCTTCCG AATCCTGGCA
    GCCGACAAGC CATACATTTT GCCCGACGAA CTGCGTCGCG AGTTGCCGCC AGACCAGGCC
    GAGTACTGCA TCCAGCGCAT GCCGCCCTAT AAGGGACCCA ACGGAGTGCC CGGCGCCCTG
    GACTACATGT CCTTCAGCAC AGCTCTCTAC GGCGAGACCG ACTTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_477484.2
    CDS399..3086
    Translation

    Target ORF information:

    RefSeq Version NM_058136.4
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster alpha actinin (Actn), transcript variant A, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_058136.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGATGATGG AGAACGGACT CTCCATGGAG TACGGAGATG GCTACATGGA GCAGGAGGAG 
    GAGTGGGAGC GCGAGGGCCT GCTCGATCCT GCGTGGGAGA AGCAACAGAA GAAGACATTT
    ACCGCCTGGT GTAACAGCCA TTTGCGCAAG GCCGGTACAG CCATCGACAA CATCGAGGAG
    GACTTCCGCA ACGGCCTCAA GCTGATGCTG CTGCTGGAGG TGATCTCCGG CGAGACGCTC
    CCCAAGCCCG ATCGCGGCAA GATGCGCTTC CACAAGATCG CCAACGTGAA CAAGGCTCTC
    GACTTCATCG CCTCCAAGGG CGTCCACCTG GTCTCTATCG GCGCCGAGGA GATCGTCGAT
    GGAAACTTGA AGATGACTCT GGGTATGATC TGGACGATTA TCCTGCGCTT CGCCATTCAG
    GACATCTCCG TGGAGGAGAT GACCGCCAAG GAGGGTCTGC TGCTGTGGTG CCAGCGCAAG
    ACGGCTCCCT ACAAGAACGT CAACGTACAG AACTTCCATC TGTCGTTCAA GGATGGTCTG
    GCCTTCTGCG CCCTTATCCA TCGCCATCGT CCAGATCTAA TCGACTACGC CAAGCTGTCC
    AAAGACAATC CTCTGGAGAA TCTTAATACT GCCTTCGATG TGGCCGAGAA GTACCTGGAT
    ATTCCGCGCA TGTTGGATCC AGATGATCTC CAGAATACAG CTTTGCCCGA TGAGCGGGCG
    GTGATGACGT ATGTGTCCTC GTACTATCAC TGCTTCAGTG GCGCCCAAAA GGCGGAAACC
    GCTGCCAACC GGATCTGCAA GGTGCTCAAG GTCAACCAGG AGAACGAACG CCTCATGGAG
    GAGTACGAGC GTCTGGCCAG TGATTTGCTG GAGTGGATCC GTCGCACCAT GCCCTGGTTG
    AACTCGCGCC AGGCCGACAA CTCGCTGGCA GGTGTGCAGA AGAAGCTGGA GGAGTACCGC
    ACCTACCGTC GCAAGCACAA GCCACCACGT GTGGAGCAGA AGGCCAAGCT GGAGACCAAC
    TTCAACACGC TGCAGACCAA GCTGCGCCTG TCCAACCGGC CCGCCTACCT GCCCACCGAG
    GGCAAGACCG TGTCCGACAT CTCCAACTCG TGGAAGGGCC TGGAGCTGGC CGAGAAGGCC
    TTTGAGGAAT GGCTGCTGGC CGAGACCATG CGCCTGGAGC GCCTTGAACA TCTGGCCCAG
    AAGTTCAAGC ACAAGGCCGA TGCCCACGAG GACTGGACGC GTGGCAAGGA GGAGATGCTG
    CAGTCGCAGG ACTTCCGCCA GTGCAAGCTC AACGAGCTGA AGGCCCTAAA GAAGAAGCAC
    GAAGCCTTTG AGTCTGACCT GGCCGCCCAC CAGGATCGTG TGGAGCAGAT TGCCGCCATC
    GCCCAGGAGC TTAACACTCT GGAGTACCAT GACTGTGTGT CTGTGAACGC CAGGTGCCAG
    CGCATCTGCG ACCAATGGGA TCGCTTGGGT GCTCTTACCC AGCGCAGGCG CACCGCCTTA
    GACGAGGCCG AGCGAATCCT GGAGAAGATC GACATCTTGC ATTTGGAGTT CGCGAAGCGT
    GCGGCGCCGT TCAACAACTG GCTGGATGGT ACGCGCGAGG ATCTTGTGGA CATGTTTATT
    GTACACACCA TGGAGGAAAT CCAAGGCCTG ATCCAGGCGC ACGACCAGTT CAAGGCGACC
    CTGGGTGAGG CCGACAAGGA GTTCAACCTG ATCGTGAACC TGGTCCGCGA AGTGGAGTCG
    ATTGTGAAGC AGCACCAGAT CCCCGGCGGC CTAGAGAACC CCTACACCAC CCTGACCGCC
    AACGACATGA CGCGCAAGTG GAGCGACGTC CGCCAACTGG TGCCCCAACG CGACCAGACT
    TTGGCCAACG AGCTGCGCAA GCAACAGAAC AACGAAATGC TCCGCCGTCA GTTTGCCGAG
    AAGGCCAACA TTGTCGGCCC CTGGATCGAG CGGCAAATGG ATGCTGTTAC GGCCATCGGT
    ATGGGACTGC AGGGATCGCT GGAGGACCAA CTGCACCGGC TCAAGGAGTA CGAGCAGGCC
    GTCTACGCCT ACAAGCCGAA CATTGAGGAG CTGGAGAAGA TCCACCAAGC GGTGCAGGAA
    TCGATGATCT TCGAGAACCG ATACACCAAC TACACGATGG AAACGCTGCG CGTCGGCTGG
    GAGCAGCTGC TTACATCTAT CAACCGCAAC ATCAACGAGG TGGAGAACCA GATTCTCACC
    CGCGACTCCA AGGGCATTAG CCAGGAGCAG CTGAACGAAT TCCGCAGCAG CTTCAACCAC
    TTCGACAAGA ACCGCACCGG CCGACTGTCG CCCGAGGAGT TCAAGTCGTG CTTGGTCTCG
    TTGGGCTACT CGATTGGCAA GGATCGCCAG GGTGATTTGG ACTTCCAGCG CATCCTCGCC
    GTCGTCGATC CCAACAACAC TGGCTACGTG CACTTCGACG CCTTCCTCGA CTTCATGACC
    CGCGAGAGCA CCGATACCGA CACTGCCGAA CAAGTCATCG ACTCCTTCCG AATCCTGGCA
    GCCGACAAGC CATACATTTT GCCCGACGAA CTGCGTCGCG AGTTGCCGCC AGACCAGGCC
    GAGTACTGCA TCCAGCGCAT GCCGCCCTAT AAGGGACCCA ACGGAGTGCC CGGCGCCCTG
    GACTACATGT CCTTCAGCAC AGCTCTCTAC GGCGAGACCG ACTTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa00544
    Clone ID Related Accession (Same CDS sequence) NM_166920.2 , NM_001201603.2 , NM_001201604.2
    Accession Version NM_166920.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2688bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product alpha actinin, isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 31, 2011 this sequence version replaced NM_166920.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGATGATGG AGAACGGACT CTCCATGGAG TACGGAGATG GCTACATGGA GCAGGAGGAG 
    GAGTGGGAGC GCGAGGGCCT GCTCGATCCT GCGTGGGAGA AGCAACAGAA GAAGACATTT
    ACCGCCTGGT GTAACAGCCA TTTGCGCAAG GCCGGTACAG CCATCGACAA CATCGAGGAG
    GACTTCCGCA ACGGCCTCAA GCTGATGCTG CTGCTGGAGG TGATCTCCGG CGAGACGCTC
    CCCAAGCCCG ATCGCGGCAA GATGCGCTTC CACAAGATCG CCAACGTGAA CAAGGCTCTC
    GACTTCATCG CCTCCAAGGG CGTCCACCTG GTCTCTATCG GCGCCGAGGA GATCGTCGAT
    GGAAACTTGA AGATGACTCT GGGTATGATC TGGACGATTA TCCTGCGCTT CGCCATTCAG
    GACATCTCCG TGGAGGAGAT GACCGCCAAG GAGGGTCTGC TGCTGTGGTG CCAGCGCAAG
    ACGGCTCCCT ACAAGAACGT CAACGTACAG AACTTCCATC TGTCGTTCAA GGATGGTCTG
    GCCTTCTGCG CCCTTATCCA TCGCCATCGT CCAGATCTAA TCGACTACGC CAAGCTGTCC
    AAAGACAATC CTCTGGAGAA TCTTAATACT GCCTTCGATG TGGCCGAGAA GTACCTGGAT
    ATTCCGCGCA TGTTGGATCC AGATGATTTG ATCAACACTC CGAAACCGGA TGAACGTGCC
    ATCATGACGT ACGTCTCCTG CTACTACCAC GCCTTCCAGG GAGCTCAACA GGCGGAAACC
    GCTGCCAACC GGATCTGCAA GGTGCTCAAG GTCAACCAGG AGAACGAACG CCTCATGGAG
    GAGTACGAGC GTCTGGCCAG TGATTTGCTG GAGTGGATCC GTCGCACCAT GCCCTGGTTG
    AACTCGCGCC AGGCCGACAA CTCGCTGGCA GGTGTGCAGA AGAAGCTGGA GGAGTACCGC
    ACCTACCGTC GCAAGCACAA GCCACCACGT GTGGAGCAGA AGGCCAAGCT GGAGACCAAC
    TTCAACACGC TGCAGACCAA GCTGCGCCTG TCCAACCGGC CCGCCTACCT GCCCACCGAG
    GGCAAGACCG TGTCCGACAT CTCCAACTCG TGGAAGGGCC TGGAGCTGGC CGAGAAGGCC
    TTTGAGGAAT GGCTGCTGGC CGAGACCATG CGCCTGGAGC GCCTTGAACA TCTGGCCCAG
    AAGTTCAAGC ACAAGGCCGA TGCCCACGAG GACTGGACGC GTGGCAAGGA GGAGATGCTG
    CAGTCGCAGG ACTTCCGCCA GTGCAAGCTC AACGAGCTGA AGGCCCTAAA GAAGAAGCAC
    GAAGCCTTTG AGTCTGACCT GGCCGCCCAC CAGGATCGTG TGGAGCAGAT TGCCGCCATC
    GCCCAGGAGC TTAACACTCT GGAGTACCAT GACTGTGTGT CTGTGAACGC CAGGTGCCAG
    CGCATCTGCG ACCAATGGGA TCGCTTGGGT GCTCTTACCC AGCGCAGGCG CACCGCCTTA
    GACGAGGCCG AGCGAATCCT GGAGAAGATC GACATCTTGC ATTTGGAGTT CGCGAAGCGT
    GCGGCGCCGT TCAACAACTG GCTGGATGGT ACGCGCGAGG ATCTTGTGGA CATGTTTATT
    GTACACACCA TGGAGGAAAT CCAAGGCCTG ATCCAGGCGC ACGACCAGTT CAAGGCGACC
    CTGGGTGAGG CCGACAAGGA GTTCAACCTG ATCGTGAACC TGGTCCGCGA AGTGGAGTCG
    ATTGTGAAGC AGCACCAGAT CCCCGGCGGC CTAGAGAACC CCTACACCAC CCTGACCGCC
    AACGACATGA CGCGCAAGTG GAGCGACGTC CGCCAACTGG TGCCCCAACG CGACCAGACT
    TTGGCCAACG AGCTGCGCAA GCAACAGAAC AACGAAATGC TCCGCCGTCA GTTTGCCGAG
    AAGGCCAACA TTGTCGGCCC CTGGATCGAG CGGCAAATGG ATGCTGTTAC GGCCATCGGT
    ATGGGACTGC AGGGATCGCT GGAGGACCAA CTGCACCGGC TCAAGGAGTA CGAGCAGGCC
    GTCTACGCCT ACAAGCCGAA CATTGAGGAG CTGGAGAAGA TCCACCAAGC GGTGCAGGAA
    TCGATGATCT TCGAGAACCG ATACACCAAC TACACGATGG AAACGCTGCG CGTCGGCTGG
    GAGCAGCTGC TTACATCTAT CAACCGCAAC ATCAACGAGG TGGAGAACCA GATTCTCACC
    CGCGACTCCA AGGGCATTAG CCAGGAGCAG CTGAACGAAT TCCGCAGCAG CTTCAACCAC
    TTCGACAAGA ACCGCACCGG CCGACTGTCG CCCGAGGAGT TCAAGTCGTG CTTGGTCTCG
    TTGGGCTACT CGATTGGCAA GGATCGCCAG GGTGATTTGG ACTTCCAGCG CATCCTCGCC
    GTCGTCGATC CCAACAACAC TGGCTACGTG CACTTCGACG CCTTCCTCGA CTTCATGACC
    CGCGAGAGCA CCGATACCGA CACTGCCGAA CAAGTCATCG ACTCCTTCCG AATCCTGGCA
    GCCGACAAGC CATACATTTT GCCCGACGAA CTGCGTCGCG AGTTGCCGCC AGACCAGGCC
    GAGTACTGCA TCCAGCGCAT GCCGCCCTAT AAGGGACCCA ACGGAGTGCC CGGCGCCCTG
    GACTACATGT CCTTCAGCAC AGCTCTCTAC GGCGAGACCG ACTTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_726784.1
    CDS399..3086
    Translation

    Target ORF information:

    RefSeq Version NM_166920.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster alpha actinin (Actn), transcript variant B, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_166920.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGATGATGG AGAACGGACT CTCCATGGAG TACGGAGATG GCTACATGGA GCAGGAGGAG 
    GAGTGGGAGC GCGAGGGCCT GCTCGATCCT GCGTGGGAGA AGCAACAGAA GAAGACATTT
    ACCGCCTGGT GTAACAGCCA TTTGCGCAAG GCCGGTACAG CCATCGACAA CATCGAGGAG
    GACTTCCGCA ACGGCCTCAA GCTGATGCTG CTGCTGGAGG TGATCTCCGG CGAGACGCTC
    CCCAAGCCCG ATCGCGGCAA GATGCGCTTC CACAAGATCG CCAACGTGAA CAAGGCTCTC
    GACTTCATCG CCTCCAAGGG CGTCCACCTG GTCTCTATCG GCGCCGAGGA GATCGTCGAT
    GGAAACTTGA AGATGACTCT GGGTATGATC TGGACGATTA TCCTGCGCTT CGCCATTCAG
    GACATCTCCG TGGAGGAGAT GACCGCCAAG GAGGGTCTGC TGCTGTGGTG CCAGCGCAAG
    ACGGCTCCCT ACAAGAACGT CAACGTACAG AACTTCCATC TGTCGTTCAA GGATGGTCTG
    GCCTTCTGCG CCCTTATCCA TCGCCATCGT CCAGATCTAA TCGACTACGC CAAGCTGTCC
    AAAGACAATC CTCTGGAGAA TCTTAATACT GCCTTCGATG TGGCCGAGAA GTACCTGGAT
    ATTCCGCGCA TGTTGGATCC AGATGATTTG ATCAACACTC CGAAACCGGA TGAACGTGCC
    ATCATGACGT ACGTCTCCTG CTACTACCAC GCCTTCCAGG GAGCTCAACA GGCGGAAACC
    GCTGCCAACC GGATCTGCAA GGTGCTCAAG GTCAACCAGG AGAACGAACG CCTCATGGAG
    GAGTACGAGC GTCTGGCCAG TGATTTGCTG GAGTGGATCC GTCGCACCAT GCCCTGGTTG
    AACTCGCGCC AGGCCGACAA CTCGCTGGCA GGTGTGCAGA AGAAGCTGGA GGAGTACCGC
    ACCTACCGTC GCAAGCACAA GCCACCACGT GTGGAGCAGA AGGCCAAGCT GGAGACCAAC
    TTCAACACGC TGCAGACCAA GCTGCGCCTG TCCAACCGGC CCGCCTACCT GCCCACCGAG
    GGCAAGACCG TGTCCGACAT CTCCAACTCG TGGAAGGGCC TGGAGCTGGC CGAGAAGGCC
    TTTGAGGAAT GGCTGCTGGC CGAGACCATG CGCCTGGAGC GCCTTGAACA TCTGGCCCAG
    AAGTTCAAGC ACAAGGCCGA TGCCCACGAG GACTGGACGC GTGGCAAGGA GGAGATGCTG
    CAGTCGCAGG ACTTCCGCCA GTGCAAGCTC AACGAGCTGA AGGCCCTAAA GAAGAAGCAC
    GAAGCCTTTG AGTCTGACCT GGCCGCCCAC CAGGATCGTG TGGAGCAGAT TGCCGCCATC
    GCCCAGGAGC TTAACACTCT GGAGTACCAT GACTGTGTGT CTGTGAACGC CAGGTGCCAG
    CGCATCTGCG ACCAATGGGA TCGCTTGGGT GCTCTTACCC AGCGCAGGCG CACCGCCTTA
    GACGAGGCCG AGCGAATCCT GGAGAAGATC GACATCTTGC ATTTGGAGTT CGCGAAGCGT
    GCGGCGCCGT TCAACAACTG GCTGGATGGT ACGCGCGAGG ATCTTGTGGA CATGTTTATT
    GTACACACCA TGGAGGAAAT CCAAGGCCTG ATCCAGGCGC ACGACCAGTT CAAGGCGACC
    CTGGGTGAGG CCGACAAGGA GTTCAACCTG ATCGTGAACC TGGTCCGCGA AGTGGAGTCG
    ATTGTGAAGC AGCACCAGAT CCCCGGCGGC CTAGAGAACC CCTACACCAC CCTGACCGCC
    AACGACATGA CGCGCAAGTG GAGCGACGTC CGCCAACTGG TGCCCCAACG CGACCAGACT
    TTGGCCAACG AGCTGCGCAA GCAACAGAAC AACGAAATGC TCCGCCGTCA GTTTGCCGAG
    AAGGCCAACA TTGTCGGCCC CTGGATCGAG CGGCAAATGG ATGCTGTTAC GGCCATCGGT
    ATGGGACTGC AGGGATCGCT GGAGGACCAA CTGCACCGGC TCAAGGAGTA CGAGCAGGCC
    GTCTACGCCT ACAAGCCGAA CATTGAGGAG CTGGAGAAGA TCCACCAAGC GGTGCAGGAA
    TCGATGATCT TCGAGAACCG ATACACCAAC TACACGATGG AAACGCTGCG CGTCGGCTGG
    GAGCAGCTGC TTACATCTAT CAACCGCAAC ATCAACGAGG TGGAGAACCA GATTCTCACC
    CGCGACTCCA AGGGCATTAG CCAGGAGCAG CTGAACGAAT TCCGCAGCAG CTTCAACCAC
    TTCGACAAGA ACCGCACCGG CCGACTGTCG CCCGAGGAGT TCAAGTCGTG CTTGGTCTCG
    TTGGGCTACT CGATTGGCAA GGATCGCCAG GGTGATTTGG ACTTCCAGCG CATCCTCGCC
    GTCGTCGATC CCAACAACAC TGGCTACGTG CACTTCGACG CCTTCCTCGA CTTCATGACC
    CGCGAGAGCA CCGATACCGA CACTGCCGAA CAAGTCATCG ACTCCTTCCG AATCCTGGCA
    GCCGACAAGC CATACATTTT GCCCGACGAA CTGCGTCGCG AGTTGCCGCC AGACCAGGCC
    GAGTACTGCA TCCAGCGCAT GCCGCCCTAT AAGGGACCCA ACGGAGTGCC CGGCGCCCTG
    GACTACATGT CCTTCAGCAC AGCTCTCTAC GGCGAGACCG ACTTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa00544
    Clone ID Related Accession (Same CDS sequence) NM_166920.2 , NM_001201603.2 , NM_001201604.2
    Accession Version NM_001201603.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2688bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product alpha actinin, isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_001201603.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGATGATGG AGAACGGACT CTCCATGGAG TACGGAGATG GCTACATGGA GCAGGAGGAG 
    GAGTGGGAGC GCGAGGGCCT GCTCGATCCT GCGTGGGAGA AGCAACAGAA GAAGACATTT
    ACCGCCTGGT GTAACAGCCA TTTGCGCAAG GCCGGTACAG CCATCGACAA CATCGAGGAG
    GACTTCCGCA ACGGCCTCAA GCTGATGCTG CTGCTGGAGG TGATCTCCGG CGAGACGCTC
    CCCAAGCCCG ATCGCGGCAA GATGCGCTTC CACAAGATCG CCAACGTGAA CAAGGCTCTC
    GACTTCATCG CCTCCAAGGG CGTCCACCTG GTCTCTATCG GCGCCGAGGA GATCGTCGAT
    GGAAACTTGA AGATGACTCT GGGTATGATC TGGACGATTA TCCTGCGCTT CGCCATTCAG
    GACATCTCCG TGGAGGAGAT GACCGCCAAG GAGGGTCTGC TGCTGTGGTG CCAGCGCAAG
    ACGGCTCCCT ACAAGAACGT CAACGTACAG AACTTCCATC TGTCGTTCAA GGATGGTCTG
    GCCTTCTGCG CCCTTATCCA TCGCCATCGT CCAGATCTAA TCGACTACGC CAAGCTGTCC
    AAAGACAATC CTCTGGAGAA TCTTAATACT GCCTTCGATG TGGCCGAGAA GTACCTGGAT
    ATTCCGCGCA TGTTGGATCC AGATGATTTG ATCAACACTC CGAAACCGGA TGAACGTGCC
    ATCATGACGT ACGTCTCCTG CTACTACCAC GCCTTCCAGG GAGCTCAACA GGCGGAAACC
    GCTGCCAACC GGATCTGCAA GGTGCTCAAG GTCAACCAGG AGAACGAACG CCTCATGGAG
    GAGTACGAGC GTCTGGCCAG TGATTTGCTG GAGTGGATCC GTCGCACCAT GCCCTGGTTG
    AACTCGCGCC AGGCCGACAA CTCGCTGGCA GGTGTGCAGA AGAAGCTGGA GGAGTACCGC
    ACCTACCGTC GCAAGCACAA GCCACCACGT GTGGAGCAGA AGGCCAAGCT GGAGACCAAC
    TTCAACACGC TGCAGACCAA GCTGCGCCTG TCCAACCGGC CCGCCTACCT GCCCACCGAG
    GGCAAGACCG TGTCCGACAT CTCCAACTCG TGGAAGGGCC TGGAGCTGGC CGAGAAGGCC
    TTTGAGGAAT GGCTGCTGGC CGAGACCATG CGCCTGGAGC GCCTTGAACA TCTGGCCCAG
    AAGTTCAAGC ACAAGGCCGA TGCCCACGAG GACTGGACGC GTGGCAAGGA GGAGATGCTG
    CAGTCGCAGG ACTTCCGCCA GTGCAAGCTC AACGAGCTGA AGGCCCTAAA GAAGAAGCAC
    GAAGCCTTTG AGTCTGACCT GGCCGCCCAC CAGGATCGTG TGGAGCAGAT TGCCGCCATC
    GCCCAGGAGC TTAACACTCT GGAGTACCAT GACTGTGTGT CTGTGAACGC CAGGTGCCAG
    CGCATCTGCG ACCAATGGGA TCGCTTGGGT GCTCTTACCC AGCGCAGGCG CACCGCCTTA
    GACGAGGCCG AGCGAATCCT GGAGAAGATC GACATCTTGC ATTTGGAGTT CGCGAAGCGT
    GCGGCGCCGT TCAACAACTG GCTGGATGGT ACGCGCGAGG ATCTTGTGGA CATGTTTATT
    GTACACACCA TGGAGGAAAT CCAAGGCCTG ATCCAGGCGC ACGACCAGTT CAAGGCGACC
    CTGGGTGAGG CCGACAAGGA GTTCAACCTG ATCGTGAACC TGGTCCGCGA AGTGGAGTCG
    ATTGTGAAGC AGCACCAGAT CCCCGGCGGC CTAGAGAACC CCTACACCAC CCTGACCGCC
    AACGACATGA CGCGCAAGTG GAGCGACGTC CGCCAACTGG TGCCCCAACG CGACCAGACT
    TTGGCCAACG AGCTGCGCAA GCAACAGAAC AACGAAATGC TCCGCCGTCA GTTTGCCGAG
    AAGGCCAACA TTGTCGGCCC CTGGATCGAG CGGCAAATGG ATGCTGTTAC GGCCATCGGT
    ATGGGACTGC AGGGATCGCT GGAGGACCAA CTGCACCGGC TCAAGGAGTA CGAGCAGGCC
    GTCTACGCCT ACAAGCCGAA CATTGAGGAG CTGGAGAAGA TCCACCAAGC GGTGCAGGAA
    TCGATGATCT TCGAGAACCG ATACACCAAC TACACGATGG AAACGCTGCG CGTCGGCTGG
    GAGCAGCTGC TTACATCTAT CAACCGCAAC ATCAACGAGG TGGAGAACCA GATTCTCACC
    CGCGACTCCA AGGGCATTAG CCAGGAGCAG CTGAACGAAT TCCGCAGCAG CTTCAACCAC
    TTCGACAAGA ACCGCACCGG CCGACTGTCG CCCGAGGAGT TCAAGTCGTG CTTGGTCTCG
    TTGGGCTACT CGATTGGCAA GGATCGCCAG GGTGATTTGG ACTTCCAGCG CATCCTCGCC
    GTCGTCGATC CCAACAACAC TGGCTACGTG CACTTCGACG CCTTCCTCGA CTTCATGACC
    CGCGAGAGCA CCGATACCGA CACTGCCGAA CAAGTCATCG ACTCCTTCCG AATCCTGGCA
    GCCGACAAGC CATACATTTT GCCCGACGAA CTGCGTCGCG AGTTGCCGCC AGACCAGGCC
    GAGTACTGCA TCCAGCGCAT GCCGCCCTAT AAGGGACCCA ACGGAGTGCC CGGCGCCCTG
    GACTACATGT CCTTCAGCAC AGCTCTCTAC GGCGAGACCG ACTTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001188532.1
    CDS194..2881
    Translation

    Target ORF information:

    RefSeq Version NM_001201603.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster alpha actinin (Actn), transcript variant D, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001201603.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGATGATGG AGAACGGACT CTCCATGGAG TACGGAGATG GCTACATGGA GCAGGAGGAG 
    GAGTGGGAGC GCGAGGGCCT GCTCGATCCT GCGTGGGAGA AGCAACAGAA GAAGACATTT
    ACCGCCTGGT GTAACAGCCA TTTGCGCAAG GCCGGTACAG CCATCGACAA CATCGAGGAG
    GACTTCCGCA ACGGCCTCAA GCTGATGCTG CTGCTGGAGG TGATCTCCGG CGAGACGCTC
    CCCAAGCCCG ATCGCGGCAA GATGCGCTTC CACAAGATCG CCAACGTGAA CAAGGCTCTC
    GACTTCATCG CCTCCAAGGG CGTCCACCTG GTCTCTATCG GCGCCGAGGA GATCGTCGAT
    GGAAACTTGA AGATGACTCT GGGTATGATC TGGACGATTA TCCTGCGCTT CGCCATTCAG
    GACATCTCCG TGGAGGAGAT GACCGCCAAG GAGGGTCTGC TGCTGTGGTG CCAGCGCAAG
    ACGGCTCCCT ACAAGAACGT CAACGTACAG AACTTCCATC TGTCGTTCAA GGATGGTCTG
    GCCTTCTGCG CCCTTATCCA TCGCCATCGT CCAGATCTAA TCGACTACGC CAAGCTGTCC
    AAAGACAATC CTCTGGAGAA TCTTAATACT GCCTTCGATG TGGCCGAGAA GTACCTGGAT
    ATTCCGCGCA TGTTGGATCC AGATGATTTG ATCAACACTC CGAAACCGGA TGAACGTGCC
    ATCATGACGT ACGTCTCCTG CTACTACCAC GCCTTCCAGG GAGCTCAACA GGCGGAAACC
    GCTGCCAACC GGATCTGCAA GGTGCTCAAG GTCAACCAGG AGAACGAACG CCTCATGGAG
    GAGTACGAGC GTCTGGCCAG TGATTTGCTG GAGTGGATCC GTCGCACCAT GCCCTGGTTG
    AACTCGCGCC AGGCCGACAA CTCGCTGGCA GGTGTGCAGA AGAAGCTGGA GGAGTACCGC
    ACCTACCGTC GCAAGCACAA GCCACCACGT GTGGAGCAGA AGGCCAAGCT GGAGACCAAC
    TTCAACACGC TGCAGACCAA GCTGCGCCTG TCCAACCGGC CCGCCTACCT GCCCACCGAG
    GGCAAGACCG TGTCCGACAT CTCCAACTCG TGGAAGGGCC TGGAGCTGGC CGAGAAGGCC
    TTTGAGGAAT GGCTGCTGGC CGAGACCATG CGCCTGGAGC GCCTTGAACA TCTGGCCCAG
    AAGTTCAAGC ACAAGGCCGA TGCCCACGAG GACTGGACGC GTGGCAAGGA GGAGATGCTG
    CAGTCGCAGG ACTTCCGCCA GTGCAAGCTC AACGAGCTGA AGGCCCTAAA GAAGAAGCAC
    GAAGCCTTTG AGTCTGACCT GGCCGCCCAC CAGGATCGTG TGGAGCAGAT TGCCGCCATC
    GCCCAGGAGC TTAACACTCT GGAGTACCAT GACTGTGTGT CTGTGAACGC CAGGTGCCAG
    CGCATCTGCG ACCAATGGGA TCGCTTGGGT GCTCTTACCC AGCGCAGGCG CACCGCCTTA
    GACGAGGCCG AGCGAATCCT GGAGAAGATC GACATCTTGC ATTTGGAGTT CGCGAAGCGT
    GCGGCGCCGT TCAACAACTG GCTGGATGGT ACGCGCGAGG ATCTTGTGGA CATGTTTATT
    GTACACACCA TGGAGGAAAT CCAAGGCCTG ATCCAGGCGC ACGACCAGTT CAAGGCGACC
    CTGGGTGAGG CCGACAAGGA GTTCAACCTG ATCGTGAACC TGGTCCGCGA AGTGGAGTCG
    ATTGTGAAGC AGCACCAGAT CCCCGGCGGC CTAGAGAACC CCTACACCAC CCTGACCGCC
    AACGACATGA CGCGCAAGTG GAGCGACGTC CGCCAACTGG TGCCCCAACG CGACCAGACT
    TTGGCCAACG AGCTGCGCAA GCAACAGAAC AACGAAATGC TCCGCCGTCA GTTTGCCGAG
    AAGGCCAACA TTGTCGGCCC CTGGATCGAG CGGCAAATGG ATGCTGTTAC GGCCATCGGT
    ATGGGACTGC AGGGATCGCT GGAGGACCAA CTGCACCGGC TCAAGGAGTA CGAGCAGGCC
    GTCTACGCCT ACAAGCCGAA CATTGAGGAG CTGGAGAAGA TCCACCAAGC GGTGCAGGAA
    TCGATGATCT TCGAGAACCG ATACACCAAC TACACGATGG AAACGCTGCG CGTCGGCTGG
    GAGCAGCTGC TTACATCTAT CAACCGCAAC ATCAACGAGG TGGAGAACCA GATTCTCACC
    CGCGACTCCA AGGGCATTAG CCAGGAGCAG CTGAACGAAT TCCGCAGCAG CTTCAACCAC
    TTCGACAAGA ACCGCACCGG CCGACTGTCG CCCGAGGAGT TCAAGTCGTG CTTGGTCTCG
    TTGGGCTACT CGATTGGCAA GGATCGCCAG GGTGATTTGG ACTTCCAGCG CATCCTCGCC
    GTCGTCGATC CCAACAACAC TGGCTACGTG CACTTCGACG CCTTCCTCGA CTTCATGACC
    CGCGAGAGCA CCGATACCGA CACTGCCGAA CAAGTCATCG ACTCCTTCCG AATCCTGGCA
    GCCGACAAGC CATACATTTT GCCCGACGAA CTGCGTCGCG AGTTGCCGCC AGACCAGGCC
    GAGTACTGCA TCCAGCGCAT GCCGCCCTAT AAGGGACCCA ACGGAGTGCC CGGCGCCCTG
    GACTACATGT CCTTCAGCAC AGCTCTCTAC GGCGAGACCG ACTTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa00544
    Clone ID Related Accession (Same CDS sequence) NM_166920.2 , NM_001201603.2 , NM_001201604.2
    Accession Version NM_001201604.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2688bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product alpha actinin, isoform I
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jan 16, 2013 this sequence version replaced NM_001201604.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGATGATGG AGAACGGACT CTCCATGGAG TACGGAGATG GCTACATGGA GCAGGAGGAG 
    GAGTGGGAGC GCGAGGGCCT GCTCGATCCT GCGTGGGAGA AGCAACAGAA GAAGACATTT
    ACCGCCTGGT GTAACAGCCA TTTGCGCAAG GCCGGTACAG CCATCGACAA CATCGAGGAG
    GACTTCCGCA ACGGCCTCAA GCTGATGCTG CTGCTGGAGG TGATCTCCGG CGAGACGCTC
    CCCAAGCCCG ATCGCGGCAA GATGCGCTTC CACAAGATCG CCAACGTGAA CAAGGCTCTC
    GACTTCATCG CCTCCAAGGG CGTCCACCTG GTCTCTATCG GCGCCGAGGA GATCGTCGAT
    GGAAACTTGA AGATGACTCT GGGTATGATC TGGACGATTA TCCTGCGCTT CGCCATTCAG
    GACATCTCCG TGGAGGAGAT GACCGCCAAG GAGGGTCTGC TGCTGTGGTG CCAGCGCAAG
    ACGGCTCCCT ACAAGAACGT CAACGTACAG AACTTCCATC TGTCGTTCAA GGATGGTCTG
    GCCTTCTGCG CCCTTATCCA TCGCCATCGT CCAGATCTAA TCGACTACGC CAAGCTGTCC
    AAAGACAATC CTCTGGAGAA TCTTAATACT GCCTTCGATG TGGCCGAGAA GTACCTGGAT
    ATTCCGCGCA TGTTGGATCC AGATGATTTG ATCAACACTC CGAAACCGGA TGAACGTGCC
    ATCATGACGT ACGTCTCCTG CTACTACCAC GCCTTCCAGG GAGCTCAACA GGCGGAAACC
    GCTGCCAACC GGATCTGCAA GGTGCTCAAG GTCAACCAGG AGAACGAACG CCTCATGGAG
    GAGTACGAGC GTCTGGCCAG TGATTTGCTG GAGTGGATCC GTCGCACCAT GCCCTGGTTG
    AACTCGCGCC AGGCCGACAA CTCGCTGGCA GGTGTGCAGA AGAAGCTGGA GGAGTACCGC
    ACCTACCGTC GCAAGCACAA GCCACCACGT GTGGAGCAGA AGGCCAAGCT GGAGACCAAC
    TTCAACACGC TGCAGACCAA GCTGCGCCTG TCCAACCGGC CCGCCTACCT GCCCACCGAG
    GGCAAGACCG TGTCCGACAT CTCCAACTCG TGGAAGGGCC TGGAGCTGGC CGAGAAGGCC
    TTTGAGGAAT GGCTGCTGGC CGAGACCATG CGCCTGGAGC GCCTTGAACA TCTGGCCCAG
    AAGTTCAAGC ACAAGGCCGA TGCCCACGAG GACTGGACGC GTGGCAAGGA GGAGATGCTG
    CAGTCGCAGG ACTTCCGCCA GTGCAAGCTC AACGAGCTGA AGGCCCTAAA GAAGAAGCAC
    GAAGCCTTTG AGTCTGACCT GGCCGCCCAC CAGGATCGTG TGGAGCAGAT TGCCGCCATC
    GCCCAGGAGC TTAACACTCT GGAGTACCAT GACTGTGTGT CTGTGAACGC CAGGTGCCAG
    CGCATCTGCG ACCAATGGGA TCGCTTGGGT GCTCTTACCC AGCGCAGGCG CACCGCCTTA
    GACGAGGCCG AGCGAATCCT GGAGAAGATC GACATCTTGC ATTTGGAGTT CGCGAAGCGT
    GCGGCGCCGT TCAACAACTG GCTGGATGGT ACGCGCGAGG ATCTTGTGGA CATGTTTATT
    GTACACACCA TGGAGGAAAT CCAAGGCCTG ATCCAGGCGC ACGACCAGTT CAAGGCGACC
    CTGGGTGAGG CCGACAAGGA GTTCAACCTG ATCGTGAACC TGGTCCGCGA AGTGGAGTCG
    ATTGTGAAGC AGCACCAGAT CCCCGGCGGC CTAGAGAACC CCTACACCAC CCTGACCGCC
    AACGACATGA CGCGCAAGTG GAGCGACGTC CGCCAACTGG TGCCCCAACG CGACCAGACT
    TTGGCCAACG AGCTGCGCAA GCAACAGAAC AACGAAATGC TCCGCCGTCA GTTTGCCGAG
    AAGGCCAACA TTGTCGGCCC CTGGATCGAG CGGCAAATGG ATGCTGTTAC GGCCATCGGT
    ATGGGACTGC AGGGATCGCT GGAGGACCAA CTGCACCGGC TCAAGGAGTA CGAGCAGGCC
    GTCTACGCCT ACAAGCCGAA CATTGAGGAG CTGGAGAAGA TCCACCAAGC GGTGCAGGAA
    TCGATGATCT TCGAGAACCG ATACACCAAC TACACGATGG AAACGCTGCG CGTCGGCTGG
    GAGCAGCTGC TTACATCTAT CAACCGCAAC ATCAACGAGG TGGAGAACCA GATTCTCACC
    CGCGACTCCA AGGGCATTAG CCAGGAGCAG CTGAACGAAT TCCGCAGCAG CTTCAACCAC
    TTCGACAAGA ACCGCACCGG CCGACTGTCG CCCGAGGAGT TCAAGTCGTG CTTGGTCTCG
    TTGGGCTACT CGATTGGCAA GGATCGCCAG GGTGATTTGG ACTTCCAGCG CATCCTCGCC
    GTCGTCGATC CCAACAACAC TGGCTACGTG CACTTCGACG CCTTCCTCGA CTTCATGACC
    CGCGAGAGCA CCGATACCGA CACTGCCGAA CAAGTCATCG ACTCCTTCCG AATCCTGGCA
    GCCGACAAGC CATACATTTT GCCCGACGAA CTGCGTCGCG AGTTGCCGCC AGACCAGGCC
    GAGTACTGCA TCCAGCGCAT GCCGCCCTAT AAGGGACCCA ACGGAGTGCC CGGCGCCCTG
    GACTACATGT CCTTCAGCAC AGCTCTCTAC GGCGAGACCG ACTTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001188533.1
    CDS404..3091
    Translation

    Target ORF information:

    RefSeq Version NM_001201604.2
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster alpha actinin (Actn), transcript variant I, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001201604.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGATGATGG AGAACGGACT CTCCATGGAG TACGGAGATG GCTACATGGA GCAGGAGGAG 
    GAGTGGGAGC GCGAGGGCCT GCTCGATCCT GCGTGGGAGA AGCAACAGAA GAAGACATTT
    ACCGCCTGGT GTAACAGCCA TTTGCGCAAG GCCGGTACAG CCATCGACAA CATCGAGGAG
    GACTTCCGCA ACGGCCTCAA GCTGATGCTG CTGCTGGAGG TGATCTCCGG CGAGACGCTC
    CCCAAGCCCG ATCGCGGCAA GATGCGCTTC CACAAGATCG CCAACGTGAA CAAGGCTCTC
    GACTTCATCG CCTCCAAGGG CGTCCACCTG GTCTCTATCG GCGCCGAGGA GATCGTCGAT
    GGAAACTTGA AGATGACTCT GGGTATGATC TGGACGATTA TCCTGCGCTT CGCCATTCAG
    GACATCTCCG TGGAGGAGAT GACCGCCAAG GAGGGTCTGC TGCTGTGGTG CCAGCGCAAG
    ACGGCTCCCT ACAAGAACGT CAACGTACAG AACTTCCATC TGTCGTTCAA GGATGGTCTG
    GCCTTCTGCG CCCTTATCCA TCGCCATCGT CCAGATCTAA TCGACTACGC CAAGCTGTCC
    AAAGACAATC CTCTGGAGAA TCTTAATACT GCCTTCGATG TGGCCGAGAA GTACCTGGAT
    ATTCCGCGCA TGTTGGATCC AGATGATTTG ATCAACACTC CGAAACCGGA TGAACGTGCC
    ATCATGACGT ACGTCTCCTG CTACTACCAC GCCTTCCAGG GAGCTCAACA GGCGGAAACC
    GCTGCCAACC GGATCTGCAA GGTGCTCAAG GTCAACCAGG AGAACGAACG CCTCATGGAG
    GAGTACGAGC GTCTGGCCAG TGATTTGCTG GAGTGGATCC GTCGCACCAT GCCCTGGTTG
    AACTCGCGCC AGGCCGACAA CTCGCTGGCA GGTGTGCAGA AGAAGCTGGA GGAGTACCGC
    ACCTACCGTC GCAAGCACAA GCCACCACGT GTGGAGCAGA AGGCCAAGCT GGAGACCAAC
    TTCAACACGC TGCAGACCAA GCTGCGCCTG TCCAACCGGC CCGCCTACCT GCCCACCGAG
    GGCAAGACCG TGTCCGACAT CTCCAACTCG TGGAAGGGCC TGGAGCTGGC CGAGAAGGCC
    TTTGAGGAAT GGCTGCTGGC CGAGACCATG CGCCTGGAGC GCCTTGAACA TCTGGCCCAG
    AAGTTCAAGC ACAAGGCCGA TGCCCACGAG GACTGGACGC GTGGCAAGGA GGAGATGCTG
    CAGTCGCAGG ACTTCCGCCA GTGCAAGCTC AACGAGCTGA AGGCCCTAAA GAAGAAGCAC
    GAAGCCTTTG AGTCTGACCT GGCCGCCCAC CAGGATCGTG TGGAGCAGAT TGCCGCCATC
    GCCCAGGAGC TTAACACTCT GGAGTACCAT GACTGTGTGT CTGTGAACGC CAGGTGCCAG
    CGCATCTGCG ACCAATGGGA TCGCTTGGGT GCTCTTACCC AGCGCAGGCG CACCGCCTTA
    GACGAGGCCG AGCGAATCCT GGAGAAGATC GACATCTTGC ATTTGGAGTT CGCGAAGCGT
    GCGGCGCCGT TCAACAACTG GCTGGATGGT ACGCGCGAGG ATCTTGTGGA CATGTTTATT
    GTACACACCA TGGAGGAAAT CCAAGGCCTG ATCCAGGCGC ACGACCAGTT CAAGGCGACC
    CTGGGTGAGG CCGACAAGGA GTTCAACCTG ATCGTGAACC TGGTCCGCGA AGTGGAGTCG
    ATTGTGAAGC AGCACCAGAT CCCCGGCGGC CTAGAGAACC CCTACACCAC CCTGACCGCC
    AACGACATGA CGCGCAAGTG GAGCGACGTC CGCCAACTGG TGCCCCAACG CGACCAGACT
    TTGGCCAACG AGCTGCGCAA GCAACAGAAC AACGAAATGC TCCGCCGTCA GTTTGCCGAG
    AAGGCCAACA TTGTCGGCCC CTGGATCGAG CGGCAAATGG ATGCTGTTAC GGCCATCGGT
    ATGGGACTGC AGGGATCGCT GGAGGACCAA CTGCACCGGC TCAAGGAGTA CGAGCAGGCC
    GTCTACGCCT ACAAGCCGAA CATTGAGGAG CTGGAGAAGA TCCACCAAGC GGTGCAGGAA
    TCGATGATCT TCGAGAACCG ATACACCAAC TACACGATGG AAACGCTGCG CGTCGGCTGG
    GAGCAGCTGC TTACATCTAT CAACCGCAAC ATCAACGAGG TGGAGAACCA GATTCTCACC
    CGCGACTCCA AGGGCATTAG CCAGGAGCAG CTGAACGAAT TCCGCAGCAG CTTCAACCAC
    TTCGACAAGA ACCGCACCGG CCGACTGTCG CCCGAGGAGT TCAAGTCGTG CTTGGTCTCG
    TTGGGCTACT CGATTGGCAA GGATCGCCAG GGTGATTTGG ACTTCCAGCG CATCCTCGCC
    GTCGTCGATC CCAACAACAC TGGCTACGTG CACTTCGACG CCTTCCTCGA CTTCATGACC
    CGCGAGAGCA CCGATACCGA CACTGCCGAA CAAGTCATCG ACTCCTTCCG AATCCTGGCA
    GCCGACAAGC CATACATTTT GCCCGACGAA CTGCGTCGCG AGTTGCCGCC AGACCAGGCC
    GAGTACTGCA TCCAGCGCAT GCCGCCCTAT AAGGGACCCA ACGGAGTGCC CGGCGCCCTG
    GACTACATGT CCTTCAGCAC AGCTCTCTAC GGCGAGACCG ACTTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OFa03775
    Clone ID Related Accession (Same CDS sequence) NM_001272235.1
    Accession Version NM_001272235.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2694bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Drosophila melanogaster(fruit fly)
    Product alpha actinin, isoform F
    Comment Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGATGATGG AGAACGGACT CTCCATGGAG TACGGAGATG GCTACATGGA GCAGGAGGAG 
    GAGTGGGAGC GCGAGGGCCT GCTCGATCCT GCGTGGGAGA AGCAACAGAA GAAGACATTT
    ACCGCCTGGT GTAACAGCCA TTTGCGCAAG GCCGGTACAG CCATCGACAA CATCGAGGAG
    GACTTCCGCA ACGGCCTCAA GCTGATGCTG CTGCTGGAGG TGATCTCCGG CGAGACGCTC
    CCCAAGCCCG ATCGCGGCAA GATGCGCTTC CACAAGATCG CCAACGTGAA CAAGGCTCTC
    GACTTCATCG CCTCCAAGGG CGTCCACCTG GTCTCTATCG GCGCCGAGGA GATCGTCGAT
    GGAAACTTGA AGATGACTCT GGGTATGATC TGGACGATTA TCCTGCGCTT CGCCATTCAG
    GACATCTCCG TGGAGGAGAT GACCGCCAAG GAGGGTCTGC TGCTGTGGTG CCAGCGCAAG
    ACGGCTCCCT ACAAGAACGT CAACGTACAG AACTTCCATC TGTCGTTCAA GGATGGTCTG
    GCCTTCTGCG CCCTTATCCA TCGCCATCGT CCAGATCTAA TCGACTACGC CAAGCTGTCC
    AAAGACAATC CTCTGGAGAA TCTTAATACT GCCTTCGATG TGGCCGAGAA GTACCTGGAT
    ATTCCGCGCA TGTTGGATCC AGATGATCTC CAGAATACAG CTTTGCCCGA TGAGCGGGCG
    GTGATGACGT ATGTGTCCTC GTACTATCAC TGCTTCAGTG GCGCCCAAAA GGCGGAAACC
    GCTGCCAACC GGATCTGCAA GGTGCTCAAG GTCAACCAGG AGAACGAACG CCTCATGGAG
    GAGTACGAGC GTCTGGCCAG TGATTTGCTG GAGTGGATCC GTCGCACCAT GCCCTGGTTG
    AACTCGCGCC AGGCCGACAA CTCGCTGGCA GGTGTGCAGA AGAAGCTGGA GGAGTACCGC
    ACCTACCGTC GCAAGCACAA GCCACCACGT GTGGAGCAGA AGGCCAAGCT GGAGACCAAC
    TTCAACACGC TGCAGACCAA GCTGCGCCTG TCCAACCGGC CCGCCTACCT GCCCACCGAG
    GGCAAGACCG TGTCCGACAT CTCCAACTCG TGGAAGGGCC TGGAGCTGGC CGAGAAGGCC
    TTTGAGGAAT GGCTGCTGGC CGAGACCATG CGCCTGGAGC GCCTTGAACA TCTGGCCCAG
    AAGTTCAAGC ACAAGGCCGA TGCCCACGAG GACTGGACGC GTGGCAAGGA GGAGATGCTG
    CAGTCGCAGG ACTTCCGCCA GTGCAAGCTC AACGAGCTGA AGGCCCTAAA GAAGAAGCAC
    GAAGCCTTTG AGTCTGACCT GGCCGCCCAC CAGGATCGTG TGGAGCAGAT TGCCGCCATC
    GCCCAGGAGC TTAACGTGAG CACTCTGGAG TACCATGACT GTGTGTCTGT GAACGCCAGG
    TGCCAGCGCA TCTGCGACCA ATGGGATCGC TTGGGTGCTC TTACCCAGCG CAGGCGCACC
    GCCTTAGACG AGGCCGAGCG AATCCTGGAG AAGATCGACA TCTTGCATTT GGAGTTCGCG
    AAGCGTGCGG CGCCGTTCAA CAACTGGCTG GATGGTACGC GCGAGGATCT TGTGGACATG
    TTTATTGTAC ACACCATGGA GGAAATCCAA GGCCTGATCC AGGCGCACGA CCAGTTCAAG
    GCGACCCTGG GTGAGGCCGA CAAGGAGTTC AACCTGATCG TGAACCTGGT CCGCGAAGTG
    GAGTCGATTG TGAAGCAGCA CCAGATCCCC GGCGGCCTAG AGAACCCCTA CACCACCCTG
    ACCGCCAACG ACATGACGCG CAAGTGGAGC GACGTCCGCC AACTGGTGCC CCAACGCGAC
    CAGACTTTGG CCAACGAGCT GCGCAAGCAA CAGAACAACG AAATGCTCCG CCGTCAGTTT
    GCCGAGAAGG CCAACATTGT CGGCCCCTGG ATCGAGCGGC AAATGGATGC TGTTACGGCC
    ATCGGTATGG GACTGCAGGG ATCGCTGGAG GACCAACTGC ACCGGCTCAA GGAGTACGAG
    CAGGCCGTCT ACGCCTACAA GCCGAACATT GAGGAGCTGG AGAAGATCCA CCAAGCGGTG
    CAGGAATCGA TGATCTTCGA GAACCGATAC ACCAACTACA CGATGGAAAC GCTGCGCGTC
    GGCTGGGAGC AGCTGCTTAC ATCTATCAAC CGCAACATCA ACGAGGTGGA GAACCAGATT
    CTCACCCGCG ACTCCAAGGG CATTAGCCAG GAGCAGCTGA ACGAATTCCG CAGCAGCTTC
    AACCACTTCG ACAAGAACCG CACCGGCCGA CTGTCGCCCG AGGAGTTCAA GTCGTGCTTG
    GTCTCGTTGG GCTACTCGAT TGGCAAGGAT CGCCAGGGTG ATTTGGACTT CCAGCGCATC
    CTCGCCGTCG TCGATCCCAA CAACACTGGC TACGTGCACT TCGACGCCTT CCTCGACTTC
    ATGACCCGCG AGAGCACCGA TACCGACACT GCCGAACAAG TCATCGACTC CTTCCGAATC
    CTGGCAGCCG ACAAGCCATA CATTTTGCCC GACGAACTGC GTCGCGAGTT GCCGCCAGAC
    CAGGCCGAGT ACTGCATCCA GCGCATGCCG CCCTATAAGG GACCCAACGG AGTGCCCGGC
    GCCCTGGACT ACATGTCCTT CAGCACAGCT CTCTACGGCG AGACCGACTT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001259164.1
    CDS399..3092
    Translation

    Target ORF information:

    RefSeq Version NM_001272235.1
    Organism Drosophila melanogaster(fruit fly)
    Definition Drosophila melanogaster alpha actinin (Actn), transcript variant F, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001272235.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGATGATGG AGAACGGACT CTCCATGGAG TACGGAGATG GCTACATGGA GCAGGAGGAG 
    GAGTGGGAGC GCGAGGGCCT GCTCGATCCT GCGTGGGAGA AGCAACAGAA GAAGACATTT
    ACCGCCTGGT GTAACAGCCA TTTGCGCAAG GCCGGTACAG CCATCGACAA CATCGAGGAG
    GACTTCCGCA ACGGCCTCAA GCTGATGCTG CTGCTGGAGG TGATCTCCGG CGAGACGCTC
    CCCAAGCCCG ATCGCGGCAA GATGCGCTTC CACAAGATCG CCAACGTGAA CAAGGCTCTC
    GACTTCATCG CCTCCAAGGG CGTCCACCTG GTCTCTATCG GCGCCGAGGA GATCGTCGAT
    GGAAACTTGA AGATGACTCT GGGTATGATC TGGACGATTA TCCTGCGCTT CGCCATTCAG
    GACATCTCCG TGGAGGAGAT GACCGCCAAG GAGGGTCTGC TGCTGTGGTG CCAGCGCAAG
    ACGGCTCCCT ACAAGAACGT CAACGTACAG AACTTCCATC TGTCGTTCAA GGATGGTCTG
    GCCTTCTGCG CCCTTATCCA TCGCCATCGT CCAGATCTAA TCGACTACGC CAAGCTGTCC
    AAAGACAATC CTCTGGAGAA TCTTAATACT GCCTTCGATG TGGCCGAGAA GTACCTGGAT
    ATTCCGCGCA TGTTGGATCC AGATGATCTC CAGAATACAG CTTTGCCCGA TGAGCGGGCG
    GTGATGACGT ATGTGTCCTC GTACTATCAC TGCTTCAGTG GCGCCCAAAA GGCGGAAACC
    GCTGCCAACC GGATCTGCAA GGTGCTCAAG GTCAACCAGG AGAACGAACG CCTCATGGAG
    GAGTACGAGC GTCTGGCCAG TGATTTGCTG GAGTGGATCC GTCGCACCAT GCCCTGGTTG
    AACTCGCGCC AGGCCGACAA CTCGCTGGCA GGTGTGCAGA AGAAGCTGGA GGAGTACCGC
    ACCTACCGTC GCAAGCACAA GCCACCACGT GTGGAGCAGA AGGCCAAGCT GGAGACCAAC
    TTCAACACGC TGCAGACCAA GCTGCGCCTG TCCAACCGGC CCGCCTACCT GCCCACCGAG
    GGCAAGACCG TGTCCGACAT CTCCAACTCG TGGAAGGGCC TGGAGCTGGC CGAGAAGGCC
    TTTGAGGAAT GGCTGCTGGC CGAGACCATG CGCCTGGAGC GCCTTGAACA TCTGGCCCAG
    AAGTTCAAGC ACAAGGCCGA TGCCCACGAG GACTGGACGC GTGGCAAGGA GGAGATGCTG
    CAGTCGCAGG ACTTCCGCCA GTGCAAGCTC AACGAGCTGA AGGCCCTAAA GAAGAAGCAC
    GAAGCCTTTG AGTCTGACCT GGCCGCCCAC CAGGATCGTG TGGAGCAGAT TGCCGCCATC
    GCCCAGGAGC TTAACGTGAG CACTCTGGAG TACCATGACT GTGTGTCTGT GAACGCCAGG
    TGCCAGCGCA TCTGCGACCA ATGGGATCGC TTGGGTGCTC TTACCCAGCG CAGGCGCACC
    GCCTTAGACG AGGCCGAGCG AATCCTGGAG AAGATCGACA TCTTGCATTT GGAGTTCGCG
    AAGCGTGCGG CGCCGTTCAA CAACTGGCTG GATGGTACGC GCGAGGATCT TGTGGACATG
    TTTATTGTAC ACACCATGGA GGAAATCCAA GGCCTGATCC AGGCGCACGA CCAGTTCAAG
    GCGACCCTGG GTGAGGCCGA CAAGGAGTTC AACCTGATCG TGAACCTGGT CCGCGAAGTG
    GAGTCGATTG TGAAGCAGCA CCAGATCCCC GGCGGCCTAG AGAACCCCTA CACCACCCTG
    ACCGCCAACG ACATGACGCG CAAGTGGAGC GACGTCCGCC AACTGGTGCC CCAACGCGAC
    CAGACTTTGG CCAACGAGCT GCGCAAGCAA CAGAACAACG AAATGCTCCG CCGTCAGTTT
    GCCGAGAAGG CCAACATTGT CGGCCCCTGG ATCGAGCGGC AAATGGATGC TGTTACGGCC
    ATCGGTATGG GACTGCAGGG ATCGCTGGAG GACCAACTGC ACCGGCTCAA GGAGTACGAG
    CAGGCCGTCT ACGCCTACAA GCCGAACATT GAGGAGCTGG AGAAGATCCA CCAAGCGGTG
    CAGGAATCGA TGATCTTCGA GAACCGATAC ACCAACTACA CGATGGAAAC GCTGCGCGTC
    GGCTGGGAGC AGCTGCTTAC ATCTATCAAC CGCAACATCA ACGAGGTGGA GAACCAGATT
    CTCACCCGCG ACTCCAAGGG CATTAGCCAG GAGCAGCTGA ACGAATTCCG CAGCAGCTTC
    AACCACTTCG ACAAGAACCG CACCGGCCGA CTGTCGCCCG AGGAGTTCAA GTCGTGCTTG
    GTCTCGTTGG GCTACTCGAT TGGCAAGGAT CGCCAGGGTG ATTTGGACTT CCAGCGCATC
    CTCGCCGTCG TCGATCCCAA CAACACTGGC TACGTGCACT TCGACGCCTT CCTCGACTTC
    ATGACCCGCG AGAGCACCGA TACCGACACT GCCGAACAAG TCATCGACTC CTTCCGAATC
    CTGGCAGCCG ACAAGCCATA CATTTTGCCC GACGAACTGC GTCGCGAGTT GCCGCCAGAC
    CAGGCCGAGT ACTGCATCCA GCGCATGCCG CCCTATAAGG GACCCAACGG AGTGCCCGGC
    GCCCTGGACT ACATGTCCTT CAGCACAGCT CTCTACGGCG AGACCGACTT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.