LOC569025 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC569025
Entrez Gene ID	569025
Full Name	alpha-2-macroglobulin-like protein 1
Synonyms	Sb:cb37 protein-like,a2ml1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009291711.3	XP_009289986.1	alpha-2-macroglobulin-like protein 1 isoform X3
XM_692385.8	XP_697477.6	alpha-2-macroglobulin-like protein 1 isoform X1
XM_009291710.3	XP_009289985.1	alpha-2-macroglobulin-like protein 1 isoform X2
XM_009291710.2	XP_009289985.1	alpha-2-macroglobulin-like protein 1 isoform X2
XM_009291711.2	XP_009289986.1	alpha-2-macroglobulin-like protein 1 isoform X3
XM_692385.9	XP_697477.6	alpha-2-macroglobulin-like protein 1 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	LOC100911545	XP_003749876.1
Danio rerio (zebrafish)	LOC100003785	XP_005157599.1
Danio rerio (zebrafish)	a2ml	NP_001132951.1
Danio rerio (zebrafish)	LOC100006925	XP_001921577.3
Danio rerio (zebrafish)	LOC100006895	XP_001923676.1
Danio rerio (zebrafish)	LOC100006782	XP_001345438.5
Danio rerio (zebrafish)	a2ml1	XP_697477.6
Danio rerio (zebrafish)	zgc:171446	NP_001108028.1
Danio rerio (zebrafish)	sb:cb37	XP_001345541.5
Homo sapiens (human)	A2M	NP_000005.2
	A2M	XP_001114328.1
Canis lupus familiaris (dog)	A2M	XP_534893.3
Bos taurus (cattle)	A2M	NP_001103265.1
Danio rerio (zebrafish)	zgc:171426	XP_003198073.1
Danio rerio (zebrafish)	zgc:165453	XP_001918704.3
Pan troglodytes (chimpanzee)	A2M	XP_001139819.1
Danio rerio (zebrafish)	zgc:171445	NP_001107101.1
Mus musculus (house mouse)	A2m	NP_783327.2
Rattus norvegicus (Norway rat)	A2m	NP_036620.2
Gallus gallus (chicken)	A2M	XP_416476.3
Danio rerio (zebrafish)	zgc:165518	NP_001093623.2
Danio rerio (zebrafish)	si:dkey-52d15.2	XP_001345569.3
Danio rerio (zebrafish)	si:dkey-46g23.5	NP_001188334.1
Danio rerio (zebrafish)	si:dkey-46g23.2	XP_001332356.1
Xenopus tropicalis (tropical clawed frog)	LOC100489060	XP_002938549.2
Xenopus tropicalis (tropical clawed frog)	a2m	XP_002938550.2

Gene Ontology

The following LOC569025 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC569025 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa20419	XM_692385.9 Latest version!	Danio rerio alpha-2-macroglobulin-like protein 1 (LOC569025), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa45635	XM_009291710.3 Latest version!	Danio rerio alpha-2-macroglobulin-like protein 1 (LOC569025), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa45636	XM_009291711.3 Latest version!	Danio rerio alpha-2-macroglobulin-like protein 1 (LOC569025), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
ODa45635	XM_009291710.2	Danio rerio alpha-2-macroglobulin-like protein 1 (LOC569025), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa45636	XM_009291711.2	Danio rerio alpha-2-macroglobulin-like protein 1 (LOC569025), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa20419	XM_692385.8	Danio rerio alpha-2-macroglobulin-like protein 1 (LOC569025), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC569025 ( XM_692385.8 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_697477.6 Danio rerio alpha-2-macroglobulin-like protein 1 (LOC569025), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa20419
Clone ID Related Accession (Same CDS sequence)	XM_692385.8 , XM_692385.9
Accession Version	XM_692385.8	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	alpha-2-macroglobulin-like protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_692385.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTT TGATTCACAA AAGAGGACTA TTAATAACAT GTTTTTTGTT GTTTGTCAGT 
CAAGGAACAT CTAAACCGAA TTTTATGGTG ACATTTCCAG CCATTATAAG ATCAGGCTCA 
GAGGCTAAAT TATGTATGAG CCTTCTGAAA CCAGAAAAAA ATCTGCAGCT TACCATTACT 
CTGGTTAGCA GTAACCAGAA CCGAACACTT CTGCAGGAGA AAACAGAGAA GGAATTCCAT 
CGCTGCCTTG ACTTTCAAGC TCCTGAGTCA GCGTCTGTGC AGGAGATTTA TGTGGAAGCT 
CAAGGGAAAT ACACACATCT GATCGAAAAG AGAAAAGTCA AGTTTGTTTC ACATAATCCA 
CTGACTCTAA TAAAAACTGA TAAACCAATC TACAATCCTG GAAAAACAGT TCAATTCAGG 
ATTTTCACAA TTGACTTTGA CTTTAAACCA GTTGATGAAA CCTACAAACT GATAGCAATC 
CAGGACAATC GAGGGAACAG AATTAATCAG TGGATTAATG CCACATCCAC CCATGGCAAA 
ATTCTGCAGC TTTCCCATTC ATTGAACCCA GAGGCTTCTG TTGGTTTATA TGAGCTGTCA 
GTTGAAACCA ACAAGGGAAA ATTTCTAGAA TTTTTTCAGG TTAAAGAATA TGTTCTACCG 
AAATTCGAAG TGAAAATACA TAAGCCAAAG TTAGTAAGAG GTAATGAAAA GGAAGTAAAG 
CTGGGGGTGT GCGGAAGATA CACCCATGGA CGACCTGTTC TGGGAAGAGT AAAGATGACA 
CTCTGCTCAT CCAGAATAAT CAATTCTTAT TTCGGTTCCC ATTATCAAAT TCCATTTTGT 
GTTACTGGGT CAGCAGTACT GAACAAGACT GGGTGCGCCT TTGAGATGTT CCCCTTGTCA 
TCTTTTATTC AAAAATCAGT AGTGGAATAC ATTACATCGC AGTCTCTCGA GTTCACTGCA 
TCCATTACTG AGGAGGGAAC AGGCATCACT GAGGGGGGGA CCGAAAAAGT CACATTGTCA 
TATATTATTG GTACAGTCAA ATTTATTGAC GTGCCTGATT TTTATGAGGA AGGATCAGTC 
ATAAAGGCCA AGGTTAAAGT TGAGCTATTC AATGGACAAC CAGTTTCTAA CATCAAGTTA 
GTTGTCTACG TCCCATCTGG GATTGGAGAA CTAATCACAG ACATCAATGG GCTGGCTAAT 
TTCACCATTG ATACGTCCTT TGGGATAGGT ACTACTTTAA TTTTAACGGC AAGTTTATTA 
AGCACACCAG AGTTTGTTCC ATTCAGTTTT GATTCTGCGC ACAAAACCCT GCAAAAAAAG 
CCCAAACAAG ATTCATTTAA TAAGAGCCAA CTGGATATAA AGGCACTGGA GGAACCGCTG 
AAGTGTGGGG TGGAGATTTC AGTGACCGTG CAGTACACTA TTGCTCGAGA GACTGCTGAA 
AGTGTTACCA TAGTCTATCT GGTTTTATCA AGAGCACAAA TAGTCTCCCA CGGATATGAG 
AGCATTGAGC TGAAAGGCTC TGACAGAGTA GTGAAGGGAG AAGTGACGTT TAAAGTCCCT 
TTTCGCTCTC GGATGGCTCC AGAAATACAG TTTTTGGCAT ACTGTGTGCT GCCCAGTGAG 
AATGTACTTG CTGCGTCCAA AACATTTCAA ACTGAGAAAT GTTTTGAAAA CAAGGTCTCG 
CTGCGGTTTT CACCAACTAC TGCAGTTCCA GGTGAAGAAA ACACTCTCCA GCTCTCAGCT 
CAGCCTGGTT CACTGTGTGG TATCAGTGCT GTAGATCAGA GTGTCCTGAT CCTGGAGTCA 
GGACAACGCC TGGATGCTGA CAAAGTGTTT AAAATGTTGC CAACAATGAC TTTTACATAC 
GATGTTGAAG ATCAGATGGA ATGCTTGAAA TTTAGATCCA AAAGAAGTTT GCGGTTTATG 
CCAGCTATTT CTCAATATGG AGCAGTACCA TCCTACCATA ATATGTACCC CCCACTTTCA 
GGGAGTACAG GAGATGCTGT GTTTGAGACT TTCAAGAACA TGGGATTAAA GGTGGCAACA 
AATTTGGTTA TTAGAATCCC ACATTGTATT AAATTCAGAG ATGTATTGTT TCAGAGAGCA 
TATGGTGGTT ATGGTGTTGG TTTTGGTTAT GGAGCTGTTC CACGTGGAAT AGATCCTCCT 
GCAATTCCGG CTTTAGCAGG AGCACCAGGT GCAGGAGCCC TTATTCCAGC AAGAGGTGGA 
GTCGTAGCTG AAGCTGAAAG ACTTATACCA AGCCCACTTC CAACTTTGAG CATCTCTCTC 
TTGCCTGAGG AGACAATTCG CACATTCTTT CCAGAAACTT GGATCTGGGA AATTGTGGAG 
GTTGGAGACT CTGGATCAGC TGAGGTTCCT GTCACAGTTC CTGACACCAT CACATCTTGG 
GCGACGGAGG CCTTCTGTCT GTCCTCCACA GGTCTGGGTT TGGCTCCTCC TGCTAAGCTG 
ATAGTTTTCC AGCCCTTCTT CCTGGAGCTC TCTCTGCCTT ACTCCATTGT TCGTGGGGAG 
ATCTTTGAGC TCAAGGCCAC TGTCTTCAAC TATCAGTCCA AGTGCATCAT GGTTAAAGTG 
AGTCCAGCTC CTTCCTCAGA CTACACTCTC AAAGCCTCCT CTGATGATCA GTATTCATCC 
TGTCTCTGTG CTAATGGAAG AAAAACCTTT AAATGGATCC TTACTCCTTC TGTTCTTGGA 
GTGGTGAATG TTACAGTCAG TGCAGAGGCA GAGGCGTCCC AGACTGTGTG TGACAATGAG 
ATTGTGAGTG TGCCAGAGAG AGGACGCATT GACACCGTCA CACGGAGTCT GCGTGTCAAT 
GCTGAAGGAA TTGAAAAAAC TAAAAGCCAC AGCTGGCTGC TTTGCCCCAA AGGGCAAAAT 
CACTTGGAGG AGGTAGAGCT GGCTTTTCCA CAAAATGTGA TAGAGGGTTC AGGAAGAGCC 
ACTGTTTCAG TGCTTGGAGA CTTATTGGGC CGTGCTCTGA AGAACCTTGA TGGTTTATTA 
CGAATGCCGT ACGGCTGTGG AGAACAAAAT ATTGCTGTTC TTTCTCCCAA CATTTACATT 
CTGCAGTATC TGGAGAACAC AAATCAGCTC ACATCCGCCA TTCGAGAGAG AGCCTCTGGC 
TTCCTTAAGA GCGGTTATCA GAGGCAACTG AACTATTTGA ATTTGGATGG GTCATATACA 
ACATTTGGTC AAGGTGAAGG CAACACATGG CTGACCGCGT TTGTGCTGAG GACTTTCGGG 
AAAGCACAAG ATTACATCTT CATTGACCCC CCAAAAATCG AGAAATCAAA GGAATGGCTG 
GTGAGCCATC AACGACCAGA TGGTTCATAT CAAATCAAGG GAAAACTCTT TAATAACAGA 
ATGAAGGGTG GTGTAAGTGA CAGCGTGACC ATCACTGCCT ATATAACCGC ATCACTACTT 
GAGCTGAACA TACCAGTTAC GGATCCGGCT GTCAGTAAAG GCCTTTCACA CTTAAAGCAG 
TATGCTGGTG ATATTCGTAA CCTTTACACC TCTGCACTGC TAAGCTATAC TTTCAGTTTG 
GCAAAAGACA AAGAGGTTCG GGACAGCCTT TTGAACAAGC TTAAGAACAT TGCGATTTCA 
GAAGGTCCTC TTGTCCACTG GTCTCAGTCT GCATCTGCTG ATGACTCTGA TTCTCTGGAT 
GTGGAGATCA GCTCATATGT GCTGCTAGCT GTTCTCACTG CAGATTCACT CACTACAGCT 
GATCTGGGCT TTGCTAACAG GATTGTCAGC TGGCTTGTGA AGCAGCAGAA TGCCTATGGA 
GGATTCTCCT CCACGCAGGA CACAGTGGTG GCTCTTCAGG CTCTGTCTTT GTACGCCACC 
AAAGTGTTCA GCTCTGACGG CTCCAGCACA GTGACTGTAC AGTCAGCAGG AGACTCTCAC 
CACTTTGATG TCAATCAGGA CAACAAGTTA CTGTACCAGG AGAAGCAGCT GGCCAATGTG 
CCGGGCAAAT ACAGCATTGA AGTCAAGGGC TCAGCCTGTG TGTCTGTGCA GATGTCTCTT 
TTCTACAATA TCCCTACTCC AAAGAAATCT AATAAATTAG AATTCTCTGT TAAGACTGAG 
GGAACTTGCC AGAACACAGA TTGGCAAATT CTGGACGTCA TATTAAACAT AGTATATAAG 
GGAATACAAT ATATAGAGAC CTCCAACATG ATCATTGTAG ATATGAAACT CTTGTCTGGT 
TTCAGCGCAG AACCGATTTT TATGGATGAG GTAAAGAAAG CAGTAAGTCG TGTTGAATCC 
AGTGATGAGC ATATCATCGT ATATCTAAAC GAGATTCGGA ATAACATAAA CTACCGGCTT 
CCGTTTCGTA TTAAAAGGAT CTTCTCTGTT AAAAACCTCA AGCCAGCAGT TATAAAAGTC 
TACGACTACT ACCAGACCAG TGAGCGAGCT GAAGCAGAAT ACACAAATCA CTGTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_697477.6
CDS	32..4471
Translation	MMALIHKRGLLITCFLLFVSQGTSKPNFMVTFPAIIRSGSEAKLCMSLLKPEKNLQLTITLVSSNQNRTLLQEKTEKEFHRCLDFQAPESASVQEIYVEAQGKYTHLIEKRKVKFVSHNPLTLIKTDKPIYNPGKTVQFRIFTIDFDFKPVDETYKLIAIQDNRGNRINQWINATSTHGKILQLSHSLNPEASVGLYELSVETNKGKFLEFFQVKEYVLPKFEVKIHKPKLVRGNEKEVKLGVCGRYTHGRPVLGRVKMTLCSSRIINSYFGSHYQIPFCVTGSAVLNKTGCAFEMFPLSSFIQKSVVEYITSQSLEFTASITEEGTGITEGGTEKVTLSYIIGTVKFIDVPDFYEEGSVIKAKVKVELFNGQPVSNIKLVVYVPSGIGELITDINGLANFTIDTSFGIGTTLILTASLLSTPEFVPFSFDSAHKTLQKKPKQDSFNKSQLDIKALEEPLKCGVEISVTVQYTIARETAESVTIVYLVLSRAQIVSHGYESIELKGSDRVVKGEVTFKVPFRSRMAPEIQFLAYCVLPSENVLAASKTFQTEKCFENKVSLRFSPTTAVPGEENTLQLSAQPGSLCGISAVDQSVLILESGQRLDADKVFKMLPTMTFTYDVEDQMECLKFRSKRSLRFMPAISQYGAVPSYHNMYPPLSGSTGDAVFETFKNMGLKVATNLVIRIPHCIKFRDVLFQRAYGGYGVGFGYGAVPRGIDPPAIPALAGAPGAGALIPARGGVVAEAERLIPSPLPTLSISLLPEETIRTFFPETWIWEIVEVGDSGSAEVPVTVPDTITSWATEAFCLSSTGLGLAPPAKLIVFQPFFLELSLPYSIVRGEIFELKATVFNYQSKCIMVKVSPAPSSDYTLKASSDDQYSSCLCANGRKTFKWILTPSVLGVVNVTVSAEAEASQTVCDNEIVSVPERGRIDTVTRSLRVNAEGIEKTKSHSWLLCPKGQNHLEEVELAFPQNVIEGSGRATVSVLGDLLGRALKNLDGLLRMPYGCGEQNIAVLSPNIYILQYLENTNQLTSAIRERASGFLKSGYQRQLNYLNLDGSYTTFGQGEGNTWLTAFVLRTFGKAQDYIFIDPPKIEKSKEWLVSHQRPDGSYQIKGKLFNNRMKGGVSDSVTITAYITASLLELNIPVTDPAVSKGLSHLKQYAGDIRNLYTSALLSYTFSLAKDKEVRDSLLNKLKNIAISEGPLVHWSQSASADDSDSLDVEISSYVLLAVLTADSLTTADLGFANRIVSWLVKQQNAYGGFSSTQDTVVALQALSLYATKVFSSDGSSTVTVQSAGDSHHFDVNQDNKLLYQEKQLANVPGKYSIEVKGSACVSVQMSLFYNIPTPKKSNKLEFSVKTEGTCQNTDWQILDVILNIVYKGIQYIETSNMIIVDMKLLSGFSAEPIFMDEVKKAVSRVESSDEHIIVYLNEIRNNINYRLPFRIKRIFSVKNLKPAVIKVYDYYQTSERAEAEYTNHCA

Target ORF information:

RefSeq Version	XM_692385.8
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio alpha-2-macroglobulin-like protein 1 (LOC569025), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_692385.8

ORF Insert Sequence:

ATGATGGCTT TGATTCACAA AAGAGGACTA TTAATAACAT GTTTTTTGTT GTTTGTCAGT 
CAAGGAACAT CTAAACCGAA TTTTATGGTG ACATTTCCAG CCATTATAAG ATCAGGCTCA 
GAGGCTAAAT TATGTATGAG CCTTCTGAAA CCAGAAAAAA ATCTGCAGCT TACCATTACT 
CTGGTTAGCA GTAACCAGAA CCGAACACTT CTGCAGGAGA AAACAGAGAA GGAATTCCAT 
CGCTGCCTTG ACTTTCAAGC TCCTGAGTCA GCGTCTGTGC AGGAGATTTA TGTGGAAGCT 
CAAGGGAAAT ACACACATCT GATCGAAAAG AGAAAAGTCA AGTTTGTTTC ACATAATCCA 
CTGACTCTAA TAAAAACTGA TAAACCAATC TACAATCCTG GAAAAACAGT TCAATTCAGG 
ATTTTCACAA TTGACTTTGA CTTTAAACCA GTTGATGAAA CCTACAAACT GATAGCAATC 
CAGGACAATC GAGGGAACAG AATTAATCAG TGGATTAATG CCACATCCAC CCATGGCAAA 
ATTCTGCAGC TTTCCCATTC ATTGAACCCA GAGGCTTCTG TTGGTTTATA TGAGCTGTCA 
GTTGAAACCA ACAAGGGAAA ATTTCTAGAA TTTTTTCAGG TTAAAGAATA TGTTCTACCG 
AAATTCGAAG TGAAAATACA TAAGCCAAAG TTAGTAAGAG GTAATGAAAA GGAAGTAAAG 
CTGGGGGTGT GCGGAAGATA CACCCATGGA CGACCTGTTC TGGGAAGAGT AAAGATGACA 
CTCTGCTCAT CCAGAATAAT CAATTCTTAT TTCGGTTCCC ATTATCAAAT TCCATTTTGT 
GTTACTGGGT CAGCAGTACT GAACAAGACT GGGTGCGCCT TTGAGATGTT CCCCTTGTCA 
TCTTTTATTC AAAAATCAGT AGTGGAATAC ATTACATCGC AGTCTCTCGA GTTCACTGCA 
TCCATTACTG AGGAGGGAAC AGGCATCACT GAGGGGGGGA CCGAAAAAGT CACATTGTCA 
TATATTATTG GTACAGTCAA ATTTATTGAC GTGCCTGATT TTTATGAGGA AGGATCAGTC 
ATAAAGGCCA AGGTTAAAGT TGAGCTATTC AATGGACAAC CAGTTTCTAA CATCAAGTTA 
GTTGTCTACG TCCCATCTGG GATTGGAGAA CTAATCACAG ACATCAATGG GCTGGCTAAT 
TTCACCATTG ATACGTCCTT TGGGATAGGT ACTACTTTAA TTTTAACGGC AAGTTTATTA 
AGCACACCAG AGTTTGTTCC ATTCAGTTTT GATTCTGCGC ACAAAACCCT GCAAAAAAAG 
CCCAAACAAG ATTCATTTAA TAAGAGCCAA CTGGATATAA AGGCACTGGA GGAACCGCTG 
AAGTGTGGGG TGGAGATTTC AGTGACCGTG CAGTACACTA TTGCTCGAGA GACTGCTGAA 
AGTGTTACCA TAGTCTATCT GGTTTTATCA AGAGCACAAA TAGTCTCCCA CGGATATGAG 
AGCATTGAGC TGAAAGGCTC TGACAGAGTA GTGAAGGGAG AAGTGACGTT TAAAGTCCCT 
TTTCGCTCTC GGATGGCTCC AGAAATACAG TTTTTGGCAT ACTGTGTGCT GCCCAGTGAG 
AATGTACTTG CTGCGTCCAA AACATTTCAA ACTGAGAAAT GTTTTGAAAA CAAGGTCTCG 
CTGCGGTTTT CACCAACTAC TGCAGTTCCA GGTGAAGAAA ACACTCTCCA GCTCTCAGCT 
CAGCCTGGTT CACTGTGTGG TATCAGTGCT GTAGATCAGA GTGTCCTGAT CCTGGAGTCA 
GGACAACGCC TGGATGCTGA CAAAGTGTTT AAAATGTTGC CAACAATGAC TTTTACATAC 
GATGTTGAAG ATCAGATGGA ATGCTTGAAA TTTAGATCCA AAAGAAGTTT GCGGTTTATG 
CCAGCTATTT CTCAATATGG AGCAGTACCA TCCTACCATA ATATGTACCC CCCACTTTCA 
GGGAGTACAG GAGATGCTGT GTTTGAGACT TTCAAGAACA TGGGATTAAA GGTGGCAACA 
AATTTGGTTA TTAGAATCCC ACATTGTATT AAATTCAGAG ATGTATTGTT TCAGAGAGCA 
TATGGTGGTT ATGGTGTTGG TTTTGGTTAT GGAGCTGTTC CACGTGGAAT AGATCCTCCT 
GCAATTCCGG CTTTAGCAGG AGCACCAGGT GCAGGAGCCC TTATTCCAGC AAGAGGTGGA 
GTCGTAGCTG AAGCTGAAAG ACTTATACCA AGCCCACTTC CAACTTTGAG CATCTCTCTC 
TTGCCTGAGG AGACAATTCG CACATTCTTT CCAGAAACTT GGATCTGGGA AATTGTGGAG 
GTTGGAGACT CTGGATCAGC TGAGGTTCCT GTCACAGTTC CTGACACCAT CACATCTTGG 
GCGACGGAGG CCTTCTGTCT GTCCTCCACA GGTCTGGGTT TGGCTCCTCC TGCTAAGCTG 
ATAGTTTTCC AGCCCTTCTT CCTGGAGCTC TCTCTGCCTT ACTCCATTGT TCGTGGGGAG 
ATCTTTGAGC TCAAGGCCAC TGTCTTCAAC TATCAGTCCA AGTGCATCAT GGTTAAAGTG 
AGTCCAGCTC CTTCCTCAGA CTACACTCTC AAAGCCTCCT CTGATGATCA GTATTCATCC 
TGTCTCTGTG CTAATGGAAG AAAAACCTTT AAATGGATCC TTACTCCTTC TGTTCTTGGA 
GTGGTGAATG TTACAGTCAG TGCAGAGGCA GAGGCGTCCC AGACTGTGTG TGACAATGAG 
ATTGTGAGTG TGCCAGAGAG AGGACGCATT GACACCGTCA CACGGAGTCT GCGTGTCAAT 
GCTGAAGGAA TTGAAAAAAC TAAAAGCCAC AGCTGGCTGC TTTGCCCCAA AGGGCAAAAT 
CACTTGGAGG AGGTAGAGCT GGCTTTTCCA CAAAATGTGA TAGAGGGTTC AGGAAGAGCC 
ACTGTTTCAG TGCTTGGAGA CTTATTGGGC CGTGCTCTGA AGAACCTTGA TGGTTTATTA 
CGAATGCCGT ACGGCTGTGG AGAACAAAAT ATTGCTGTTC TTTCTCCCAA CATTTACATT 
CTGCAGTATC TGGAGAACAC AAATCAGCTC ACATCCGCCA TTCGAGAGAG AGCCTCTGGC 
TTCCTTAAGA GCGGTTATCA GAGGCAACTG AACTATTTGA ATTTGGATGG GTCATATACA 
ACATTTGGTC AAGGTGAAGG CAACACATGG CTGACCGCGT TTGTGCTGAG GACTTTCGGG 
AAAGCACAAG ATTACATCTT CATTGACCCC CCAAAAATCG AGAAATCAAA GGAATGGCTG 
GTGAGCCATC AACGACCAGA TGGTTCATAT CAAATCAAGG GAAAACTCTT TAATAACAGA 
ATGAAGGGTG GTGTAAGTGA CAGCGTGACC ATCACTGCCT ATATAACCGC ATCACTACTT 
GAGCTGAACA TACCAGTTAC GGATCCGGCT GTCAGTAAAG GCCTTTCACA CTTAAAGCAG 
TATGCTGGTG ATATTCGTAA CCTTTACACC TCTGCACTGC TAAGCTATAC TTTCAGTTTG 
GCAAAAGACA AAGAGGTTCG GGACAGCCTT TTGAACAAGC TTAAGAACAT TGCGATTTCA 
GAAGGTCCTC TTGTCCACTG GTCTCAGTCT GCATCTGCTG ATGACTCTGA TTCTCTGGAT 
GTGGAGATCA GCTCATATGT GCTGCTAGCT GTTCTCACTG CAGATTCACT CACTACAGCT 
GATCTGGGCT TTGCTAACAG GATTGTCAGC TGGCTTGTGA AGCAGCAGAA TGCCTATGGA 
GGATTCTCCT CCACGCAGGA CACAGTGGTG GCTCTTCAGG CTCTGTCTTT GTACGCCACC 
AAAGTGTTCA GCTCTGACGG CTCCAGCACA GTGACTGTAC AGTCAGCAGG AGACTCTCAC 
CACTTTGATG TCAATCAGGA CAACAAGTTA CTGTACCAGG AGAAGCAGCT GGCCAATGTG 
CCGGGCAAAT ACAGCATTGA AGTCAAGGGC TCAGCCTGTG TGTCTGTGCA GATGTCTCTT 
TTCTACAATA TCCCTACTCC AAAGAAATCT AATAAATTAG AATTCTCTGT TAAGACTGAG 
GGAACTTGCC AGAACACAGA TTGGCAAATT CTGGACGTCA TATTAAACAT AGTATATAAG 
GGAATACAAT ATATAGAGAC CTCCAACATG ATCATTGTAG ATATGAAACT CTTGTCTGGT 
TTCAGCGCAG AACCGATTTT TATGGATGAG GTAAAGAAAG CAGTAAGTCG TGTTGAATCC 
AGTGATGAGC ATATCATCGT ATATCTAAAC GAGATTCGGA ATAACATAAA CTACCGGCTT 
CCGTTTCGTA TTAAAAGGAT CTTCTCTGTT AAAAACCTCA AGCCAGCAGT TATAAAAGTC 
TACGACTACT ACCAGACCAG TGAGCGAGCT GAAGCAGAAT ACACAAATCA CTGTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC569025 ( XM_692385.9 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_697477.6 Danio rerio alpha-2-macroglobulin-like protein 1 (LOC569025), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa20419
Clone ID Related Accession (Same CDS sequence)	XM_692385.8 , XM_692385.9
Accession Version	XM_692385.9 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	alpha-2-macroglobulin-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_692385.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTT TGATTCACAA AAGAGGACTA TTAATAACAT GTTTTTTGTT GTTTGTCAGT 
CAAGGAACAT CTAAACCGAA TTTTATGGTG ACATTTCCAG CCATTATAAG ATCAGGCTCA 
GAGGCTAAAT TATGTATGAG CCTTCTGAAA CCAGAAAAAA ATCTGCAGCT TACCATTACT 
CTGGTTAGCA GTAACCAGAA CCGAACACTT CTGCAGGAGA AAACAGAGAA GGAATTCCAT 
CGCTGCCTTG ACTTTCAAGC TCCTGAGTCA GCGTCTGTGC AGGAGATTTA TGTGGAAGCT 
CAAGGGAAAT ACACACATCT GATCGAAAAG AGAAAAGTCA AGTTTGTTTC ACATAATCCA 
CTGACTCTAA TAAAAACTGA TAAACCAATC TACAATCCTG GAAAAACAGT TCAATTCAGG 
ATTTTCACAA TTGACTTTGA CTTTAAACCA GTTGATGAAA CCTACAAACT GATAGCAATC 
CAGGACAATC GAGGGAACAG AATTAATCAG TGGATTAATG CCACATCCAC CCATGGCAAA 
ATTCTGCAGC TTTCCCATTC ATTGAACCCA GAGGCTTCTG TTGGTTTATA TGAGCTGTCA 
GTTGAAACCA ACAAGGGAAA ATTTCTAGAA TTTTTTCAGG TTAAAGAATA TGTTCTACCG 
AAATTCGAAG TGAAAATACA TAAGCCAAAG TTAGTAAGAG GTAATGAAAA GGAAGTAAAG 
CTGGGGGTGT GCGGAAGATA CACCCATGGA CGACCTGTTC TGGGAAGAGT AAAGATGACA 
CTCTGCTCAT CCAGAATAAT CAATTCTTAT TTCGGTTCCC ATTATCAAAT TCCATTTTGT 
GTTACTGGGT CAGCAGTACT GAACAAGACT GGGTGCGCCT TTGAGATGTT CCCCTTGTCA 
TCTTTTATTC AAAAATCAGT AGTGGAATAC ATTACATCGC AGTCTCTCGA GTTCACTGCA 
TCCATTACTG AGGAGGGAAC AGGCATCACT GAGGGGGGGA CCGAAAAAGT CACATTGTCA 
TATATTATTG GTACAGTCAA ATTTATTGAC GTGCCTGATT TTTATGAGGA AGGATCAGTC 
ATAAAGGCCA AGGTTAAAGT TGAGCTATTC AATGGACAAC CAGTTTCTAA CATCAAGTTA 
GTTGTCTACG TCCCATCTGG GATTGGAGAA CTAATCACAG ACATCAATGG GCTGGCTAAT 
TTCACCATTG ATACGTCCTT TGGGATAGGT ACTACTTTAA TTTTAACGGC AAGTTTATTA 
AGCACACCAG AGTTTGTTCC ATTCAGTTTT GATTCTGCGC ACAAAACCCT GCAAAAAAAG 
CCCAAACAAG ATTCATTTAA TAAGAGCCAA CTGGATATAA AGGCACTGGA GGAACCGCTG 
AAGTGTGGGG TGGAGATTTC AGTGACCGTG CAGTACACTA TTGCTCGAGA GACTGCTGAA 
AGTGTTACCA TAGTCTATCT GGTTTTATCA AGAGCACAAA TAGTCTCCCA CGGATATGAG 
AGCATTGAGC TGAAAGGCTC TGACAGAGTA GTGAAGGGAG AAGTGACGTT TAAAGTCCCT 
TTTCGCTCTC GGATGGCTCC AGAAATACAG TTTTTGGCAT ACTGTGTGCT GCCCAGTGAG 
AATGTACTTG CTGCGTCCAA AACATTTCAA ACTGAGAAAT GTTTTGAAAA CAAGGTCTCG 
CTGCGGTTTT CACCAACTAC TGCAGTTCCA GGTGAAGAAA ACACTCTCCA GCTCTCAGCT 
CAGCCTGGTT CACTGTGTGG TATCAGTGCT GTAGATCAGA GTGTCCTGAT CCTGGAGTCA 
GGACAACGCC TGGATGCTGA CAAAGTGTTT AAAATGTTGC CAACAATGAC TTTTACATAC 
GATGTTGAAG ATCAGATGGA ATGCTTGAAA TTTAGATCCA AAAGAAGTTT GCGGTTTATG 
CCAGCTATTT CTCAATATGG AGCAGTACCA TCCTACCATA ATATGTACCC CCCACTTTCA 
GGGAGTACAG GAGATGCTGT GTTTGAGACT TTCAAGAACA TGGGATTAAA GGTGGCAACA 
AATTTGGTTA TTAGAATCCC ACATTGTATT AAATTCAGAG ATGTATTGTT TCAGAGAGCA 
TATGGTGGTT ATGGTGTTGG TTTTGGTTAT GGAGCTGTTC CACGTGGAAT AGATCCTCCT 
GCAATTCCGG CTTTAGCAGG AGCACCAGGT GCAGGAGCCC TTATTCCAGC AAGAGGTGGA 
GTCGTAGCTG AAGCTGAAAG ACTTATACCA AGCCCACTTC CAACTTTGAG CATCTCTCTC 
TTGCCTGAGG AGACAATTCG CACATTCTTT CCAGAAACTT GGATCTGGGA AATTGTGGAG 
GTTGGAGACT CTGGATCAGC TGAGGTTCCT GTCACAGTTC CTGACACCAT CACATCTTGG 
GCGACGGAGG CCTTCTGTCT GTCCTCCACA GGTCTGGGTT TGGCTCCTCC TGCTAAGCTG 
ATAGTTTTCC AGCCCTTCTT CCTGGAGCTC TCTCTGCCTT ACTCCATTGT TCGTGGGGAG 
ATCTTTGAGC TCAAGGCCAC TGTCTTCAAC TATCAGTCCA AGTGCATCAT GGTTAAAGTG 
AGTCCAGCTC CTTCCTCAGA CTACACTCTC AAAGCCTCCT CTGATGATCA GTATTCATCC 
TGTCTCTGTG CTAATGGAAG AAAAACCTTT AAATGGATCC TTACTCCTTC TGTTCTTGGA 
GTGGTGAATG TTACAGTCAG TGCAGAGGCA GAGGCGTCCC AGACTGTGTG TGACAATGAG 
ATTGTGAGTG TGCCAGAGAG AGGACGCATT GACACCGTCA CACGGAGTCT GCGTGTCAAT 
GCTGAAGGAA TTGAAAAAAC TAAAAGCCAC AGCTGGCTGC TTTGCCCCAA AGGGCAAAAT 
CACTTGGAGG AGGTAGAGCT GGCTTTTCCA CAAAATGTGA TAGAGGGTTC AGGAAGAGCC 
ACTGTTTCAG TGCTTGGAGA CTTATTGGGC CGTGCTCTGA AGAACCTTGA TGGTTTATTA 
CGAATGCCGT ACGGCTGTGG AGAACAAAAT ATTGCTGTTC TTTCTCCCAA CATTTACATT 
CTGCAGTATC TGGAGAACAC AAATCAGCTC ACATCCGCCA TTCGAGAGAG AGCCTCTGGC 
TTCCTTAAGA GCGGTTATCA GAGGCAACTG AACTATTTGA ATTTGGATGG GTCATATACA 
ACATTTGGTC AAGGTGAAGG CAACACATGG CTGACCGCGT TTGTGCTGAG GACTTTCGGG 
AAAGCACAAG ATTACATCTT CATTGACCCC CCAAAAATCG AGAAATCAAA GGAATGGCTG 
GTGAGCCATC AACGACCAGA TGGTTCATAT CAAATCAAGG GAAAACTCTT TAATAACAGA 
ATGAAGGGTG GTGTAAGTGA CAGCGTGACC ATCACTGCCT ATATAACCGC ATCACTACTT 
GAGCTGAACA TACCAGTTAC GGATCCGGCT GTCAGTAAAG GCCTTTCACA CTTAAAGCAG 
TATGCTGGTG ATATTCGTAA CCTTTACACC TCTGCACTGC TAAGCTATAC TTTCAGTTTG 
GCAAAAGACA AAGAGGTTCG GGACAGCCTT TTGAACAAGC TTAAGAACAT TGCGATTTCA 
GAAGGTCCTC TTGTCCACTG GTCTCAGTCT GCATCTGCTG ATGACTCTGA TTCTCTGGAT 
GTGGAGATCA GCTCATATGT GCTGCTAGCT GTTCTCACTG CAGATTCACT CACTACAGCT 
GATCTGGGCT TTGCTAACAG GATTGTCAGC TGGCTTGTGA AGCAGCAGAA TGCCTATGGA 
GGATTCTCCT CCACGCAGGA CACAGTGGTG GCTCTTCAGG CTCTGTCTTT GTACGCCACC 
AAAGTGTTCA GCTCTGACGG CTCCAGCACA GTGACTGTAC AGTCAGCAGG AGACTCTCAC 
CACTTTGATG TCAATCAGGA CAACAAGTTA CTGTACCAGG AGAAGCAGCT GGCCAATGTG 
CCGGGCAAAT ACAGCATTGA AGTCAAGGGC TCAGCCTGTG TGTCTGTGCA GATGTCTCTT 
TTCTACAATA TCCCTACTCC AAAGAAATCT AATAAATTAG AATTCTCTGT TAAGACTGAG 
GGAACTTGCC AGAACACAGA TTGGCAAATT CTGGACGTCA TATTAAACAT AGTATATAAG 
GGAATACAAT ATATAGAGAC CTCCAACATG ATCATTGTAG ATATGAAACT CTTGTCTGGT 
TTCAGCGCAG AACCGATTTT TATGGATGAG GTAAAGAAAG CAGTAAGTCG TGTTGAATCC 
AGTGATGAGC ATATCATCGT ATATCTAAAC GAGATTCGGA ATAACATAAA CTACCGGCTT 
CCGTTTCGTA TTAAAAGGAT CTTCTCTGTT AAAAACCTCA AGCCAGCAGT TATAAAAGTC 
TACGACTACT ACCAGACCAG TGAGCGAGCT GAAGCAGAAT ACACAAATCA CTGTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_697477.6
CDS	45..4484
Translation	MMALIHKRGLLITCFLLFVSQGTSKPNFMVTFPAIIRSGSEAKLCMSLLKPEKNLQLTITLVSSNQNRTLLQEKTEKEFHRCLDFQAPESASVQEIYVEAQGKYTHLIEKRKVKFVSHNPLTLIKTDKPIYNPGKTVQFRIFTIDFDFKPVDETYKLIAIQDNRGNRINQWINATSTHGKILQLSHSLNPEASVGLYELSVETNKGKFLEFFQVKEYVLPKFEVKIHKPKLVRGNEKEVKLGVCGRYTHGRPVLGRVKMTLCSSRIINSYFGSHYQIPFCVTGSAVLNKTGCAFEMFPLSSFIQKSVVEYITSQSLEFTASITEEGTGITEGGTEKVTLSYIIGTVKFIDVPDFYEEGSVIKAKVKVELFNGQPVSNIKLVVYVPSGIGELITDINGLANFTIDTSFGIGTTLILTASLLSTPEFVPFSFDSAHKTLQKKPKQDSFNKSQLDIKALEEPLKCGVEISVTVQYTIARETAESVTIVYLVLSRAQIVSHGYESIELKGSDRVVKGEVTFKVPFRSRMAPEIQFLAYCVLPSENVLAASKTFQTEKCFENKVSLRFSPTTAVPGEENTLQLSAQPGSLCGISAVDQSVLILESGQRLDADKVFKMLPTMTFTYDVEDQMECLKFRSKRSLRFMPAISQYGAVPSYHNMYPPLSGSTGDAVFETFKNMGLKVATNLVIRIPHCIKFRDVLFQRAYGGYGVGFGYGAVPRGIDPPAIPALAGAPGAGALIPARGGVVAEAERLIPSPLPTLSISLLPEETIRTFFPETWIWEIVEVGDSGSAEVPVTVPDTITSWATEAFCLSSTGLGLAPPAKLIVFQPFFLELSLPYSIVRGEIFELKATVFNYQSKCIMVKVSPAPSSDYTLKASSDDQYSSCLCANGRKTFKWILTPSVLGVVNVTVSAEAEASQTVCDNEIVSVPERGRIDTVTRSLRVNAEGIEKTKSHSWLLCPKGQNHLEEVELAFPQNVIEGSGRATVSVLGDLLGRALKNLDGLLRMPYGCGEQNIAVLSPNIYILQYLENTNQLTSAIRERASGFLKSGYQRQLNYLNLDGSYTTFGQGEGNTWLTAFVLRTFGKAQDYIFIDPPKIEKSKEWLVSHQRPDGSYQIKGKLFNNRMKGGVSDSVTITAYITASLLELNIPVTDPAVSKGLSHLKQYAGDIRNLYTSALLSYTFSLAKDKEVRDSLLNKLKNIAISEGPLVHWSQSASADDSDSLDVEISSYVLLAVLTADSLTTADLGFANRIVSWLVKQQNAYGGFSSTQDTVVALQALSLYATKVFSSDGSSTVTVQSAGDSHHFDVNQDNKLLYQEKQLANVPGKYSIEVKGSACVSVQMSLFYNIPTPKKSNKLEFSVKTEGTCQNTDWQILDVILNIVYKGIQYIETSNMIIVDMKLLSGFSAEPIFMDEVKKAVSRVESSDEHIIVYLNEIRNNINYRLPFRIKRIFSVKNLKPAVIKVYDYYQTSERAEAEYTNHCA

Target ORF information:

RefSeq Version	XM_692385.9
Organism	Danio rerio(zebrafish)
Definition	Danio rerio alpha-2-macroglobulin-like protein 1 (LOC569025), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_692385.9

ORF Insert Sequence:

ATGATGGCTT TGATTCACAA AAGAGGACTA TTAATAACAT GTTTTTTGTT GTTTGTCAGT 
CAAGGAACAT CTAAACCGAA TTTTATGGTG ACATTTCCAG CCATTATAAG ATCAGGCTCA 
GAGGCTAAAT TATGTATGAG CCTTCTGAAA CCAGAAAAAA ATCTGCAGCT TACCATTACT 
CTGGTTAGCA GTAACCAGAA CCGAACACTT CTGCAGGAGA AAACAGAGAA GGAATTCCAT 
CGCTGCCTTG ACTTTCAAGC TCCTGAGTCA GCGTCTGTGC AGGAGATTTA TGTGGAAGCT 
CAAGGGAAAT ACACACATCT GATCGAAAAG AGAAAAGTCA AGTTTGTTTC ACATAATCCA 
CTGACTCTAA TAAAAACTGA TAAACCAATC TACAATCCTG GAAAAACAGT TCAATTCAGG 
ATTTTCACAA TTGACTTTGA CTTTAAACCA GTTGATGAAA CCTACAAACT GATAGCAATC 
CAGGACAATC GAGGGAACAG AATTAATCAG TGGATTAATG CCACATCCAC CCATGGCAAA 
ATTCTGCAGC TTTCCCATTC ATTGAACCCA GAGGCTTCTG TTGGTTTATA TGAGCTGTCA 
GTTGAAACCA ACAAGGGAAA ATTTCTAGAA TTTTTTCAGG TTAAAGAATA TGTTCTACCG 
AAATTCGAAG TGAAAATACA TAAGCCAAAG TTAGTAAGAG GTAATGAAAA GGAAGTAAAG 
CTGGGGGTGT GCGGAAGATA CACCCATGGA CGACCTGTTC TGGGAAGAGT AAAGATGACA 
CTCTGCTCAT CCAGAATAAT CAATTCTTAT TTCGGTTCCC ATTATCAAAT TCCATTTTGT 
GTTACTGGGT CAGCAGTACT GAACAAGACT GGGTGCGCCT TTGAGATGTT CCCCTTGTCA 
TCTTTTATTC AAAAATCAGT AGTGGAATAC ATTACATCGC AGTCTCTCGA GTTCACTGCA 
TCCATTACTG AGGAGGGAAC AGGCATCACT GAGGGGGGGA CCGAAAAAGT CACATTGTCA 
TATATTATTG GTACAGTCAA ATTTATTGAC GTGCCTGATT TTTATGAGGA AGGATCAGTC 
ATAAAGGCCA AGGTTAAAGT TGAGCTATTC AATGGACAAC CAGTTTCTAA CATCAAGTTA 
GTTGTCTACG TCCCATCTGG GATTGGAGAA CTAATCACAG ACATCAATGG GCTGGCTAAT 
TTCACCATTG ATACGTCCTT TGGGATAGGT ACTACTTTAA TTTTAACGGC AAGTTTATTA 
AGCACACCAG AGTTTGTTCC ATTCAGTTTT GATTCTGCGC ACAAAACCCT GCAAAAAAAG 
CCCAAACAAG ATTCATTTAA TAAGAGCCAA CTGGATATAA AGGCACTGGA GGAACCGCTG 
AAGTGTGGGG TGGAGATTTC AGTGACCGTG CAGTACACTA TTGCTCGAGA GACTGCTGAA 
AGTGTTACCA TAGTCTATCT GGTTTTATCA AGAGCACAAA TAGTCTCCCA CGGATATGAG 
AGCATTGAGC TGAAAGGCTC TGACAGAGTA GTGAAGGGAG AAGTGACGTT TAAAGTCCCT 
TTTCGCTCTC GGATGGCTCC AGAAATACAG TTTTTGGCAT ACTGTGTGCT GCCCAGTGAG 
AATGTACTTG CTGCGTCCAA AACATTTCAA ACTGAGAAAT GTTTTGAAAA CAAGGTCTCG 
CTGCGGTTTT CACCAACTAC TGCAGTTCCA GGTGAAGAAA ACACTCTCCA GCTCTCAGCT 
CAGCCTGGTT CACTGTGTGG TATCAGTGCT GTAGATCAGA GTGTCCTGAT CCTGGAGTCA 
GGACAACGCC TGGATGCTGA CAAAGTGTTT AAAATGTTGC CAACAATGAC TTTTACATAC 
GATGTTGAAG ATCAGATGGA ATGCTTGAAA TTTAGATCCA AAAGAAGTTT GCGGTTTATG 
CCAGCTATTT CTCAATATGG AGCAGTACCA TCCTACCATA ATATGTACCC CCCACTTTCA 
GGGAGTACAG GAGATGCTGT GTTTGAGACT TTCAAGAACA TGGGATTAAA GGTGGCAACA 
AATTTGGTTA TTAGAATCCC ACATTGTATT AAATTCAGAG ATGTATTGTT TCAGAGAGCA 
TATGGTGGTT ATGGTGTTGG TTTTGGTTAT GGAGCTGTTC CACGTGGAAT AGATCCTCCT 
GCAATTCCGG CTTTAGCAGG AGCACCAGGT GCAGGAGCCC TTATTCCAGC AAGAGGTGGA 
GTCGTAGCTG AAGCTGAAAG ACTTATACCA AGCCCACTTC CAACTTTGAG CATCTCTCTC 
TTGCCTGAGG AGACAATTCG CACATTCTTT CCAGAAACTT GGATCTGGGA AATTGTGGAG 
GTTGGAGACT CTGGATCAGC TGAGGTTCCT GTCACAGTTC CTGACACCAT CACATCTTGG 
GCGACGGAGG CCTTCTGTCT GTCCTCCACA GGTCTGGGTT TGGCTCCTCC TGCTAAGCTG 
ATAGTTTTCC AGCCCTTCTT CCTGGAGCTC TCTCTGCCTT ACTCCATTGT TCGTGGGGAG 
ATCTTTGAGC TCAAGGCCAC TGTCTTCAAC TATCAGTCCA AGTGCATCAT GGTTAAAGTG 
AGTCCAGCTC CTTCCTCAGA CTACACTCTC AAAGCCTCCT CTGATGATCA GTATTCATCC 
TGTCTCTGTG CTAATGGAAG AAAAACCTTT AAATGGATCC TTACTCCTTC TGTTCTTGGA 
GTGGTGAATG TTACAGTCAG TGCAGAGGCA GAGGCGTCCC AGACTGTGTG TGACAATGAG 
ATTGTGAGTG TGCCAGAGAG AGGACGCATT GACACCGTCA CACGGAGTCT GCGTGTCAAT 
GCTGAAGGAA TTGAAAAAAC TAAAAGCCAC AGCTGGCTGC TTTGCCCCAA AGGGCAAAAT 
CACTTGGAGG AGGTAGAGCT GGCTTTTCCA CAAAATGTGA TAGAGGGTTC AGGAAGAGCC 
ACTGTTTCAG TGCTTGGAGA CTTATTGGGC CGTGCTCTGA AGAACCTTGA TGGTTTATTA 
CGAATGCCGT ACGGCTGTGG AGAACAAAAT ATTGCTGTTC TTTCTCCCAA CATTTACATT 
CTGCAGTATC TGGAGAACAC AAATCAGCTC ACATCCGCCA TTCGAGAGAG AGCCTCTGGC 
TTCCTTAAGA GCGGTTATCA GAGGCAACTG AACTATTTGA ATTTGGATGG GTCATATACA 
ACATTTGGTC AAGGTGAAGG CAACACATGG CTGACCGCGT TTGTGCTGAG GACTTTCGGG 
AAAGCACAAG ATTACATCTT CATTGACCCC CCAAAAATCG AGAAATCAAA GGAATGGCTG 
GTGAGCCATC AACGACCAGA TGGTTCATAT CAAATCAAGG GAAAACTCTT TAATAACAGA 
ATGAAGGGTG GTGTAAGTGA CAGCGTGACC ATCACTGCCT ATATAACCGC ATCACTACTT 
GAGCTGAACA TACCAGTTAC GGATCCGGCT GTCAGTAAAG GCCTTTCACA CTTAAAGCAG 
TATGCTGGTG ATATTCGTAA CCTTTACACC TCTGCACTGC TAAGCTATAC TTTCAGTTTG 
GCAAAAGACA AAGAGGTTCG GGACAGCCTT TTGAACAAGC TTAAGAACAT TGCGATTTCA 
GAAGGTCCTC TTGTCCACTG GTCTCAGTCT GCATCTGCTG ATGACTCTGA TTCTCTGGAT 
GTGGAGATCA GCTCATATGT GCTGCTAGCT GTTCTCACTG CAGATTCACT CACTACAGCT 
GATCTGGGCT TTGCTAACAG GATTGTCAGC TGGCTTGTGA AGCAGCAGAA TGCCTATGGA 
GGATTCTCCT CCACGCAGGA CACAGTGGTG GCTCTTCAGG CTCTGTCTTT GTACGCCACC 
AAAGTGTTCA GCTCTGACGG CTCCAGCACA GTGACTGTAC AGTCAGCAGG AGACTCTCAC 
CACTTTGATG TCAATCAGGA CAACAAGTTA CTGTACCAGG AGAAGCAGCT GGCCAATGTG 
CCGGGCAAAT ACAGCATTGA AGTCAAGGGC TCAGCCTGTG TGTCTGTGCA GATGTCTCTT 
TTCTACAATA TCCCTACTCC AAAGAAATCT AATAAATTAG AATTCTCTGT TAAGACTGAG 
GGAACTTGCC AGAACACAGA TTGGCAAATT CTGGACGTCA TATTAAACAT AGTATATAAG 
GGAATACAAT ATATAGAGAC CTCCAACATG ATCATTGTAG ATATGAAACT CTTGTCTGGT 
TTCAGCGCAG AACCGATTTT TATGGATGAG GTAAAGAAAG CAGTAAGTCG TGTTGAATCC 
AGTGATGAGC ATATCATCGT ATATCTAAAC GAGATTCGGA ATAACATAAA CTACCGGCTT 
CCGTTTCGTA TTAAAAGGAT CTTCTCTGTT AAAAACCTCA AGCCAGCAGT TATAAAAGTC 
TACGACTACT ACCAGACCAG TGAGCGAGCT GAAGCAGAAT ACACAAATCA CTGTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC569025 ( XM_009291710.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009289985.1 Danio rerio alpha-2-macroglobulin-like protein 1 (LOC569025), transcript variant X2, mRNA.

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CloneID	ODa45635
Clone ID Related Accession (Same CDS sequence)	XM_009291710.3 , XM_009291710.2
Accession Version	XM_009291710.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	alpha-2-macroglobulin-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291710.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTT TGATTCACAA AAGAGGACTA TTAATAACAT GTTTTTTGTT GTTTGTCAGT 
CAAGGAACAT CTAAACCGAA TTTTATGGTG ACATTTCCAG CCATTATAAG ATCAGGCTCA 
GAGGCTAAAT TATGTATGAG CCTTCTGAAA CCAGAAAAAA ATCTGCAGCT TACCATTACT 
CTGGTTAGCA GTAACCAGAA CCGAACACTT CTGCAGGAGA AAACAGAGAA GGAATTCCAT 
CGCTGCCTTG ACTTTCAAGC TCCTGAGTCA GCGTCTGTGC AGGAGATTTA TGTGGAAGCT 
CAAGGGAAAT ACACACATCT GATCGAAAAG AGAAAAGTCA AGTTTGTTTC ACATAATCCA 
CTGACTCTAA TAAAAACTGA TAAACCAATC TACAATCCTG GAAAAACAGT TCAATTCAGG 
ATTTTCACAA TTGACTTTGA CTTTAAACCA GTTGATGAAA CCTACAAACT GATAGCAATC 
CAGGACAATC GAGGGAACAG AATTAATCAG TGGATTAATG CCACATCCAC CCATGGCAAA 
ATTCTGCAGC TTTCCCATTC ATTGAACCCA GAGGCTTCTG TTGGTTTATA TGAGCTGTCA 
GTTGAAACCA ACAAGGGAAA ATTTCTAGAA TTTTTTCAGG TTAAAGAATA TGTTCTACCG 
AAATTCGAAG TGAAAATACA TAAGCCAAAG TTAGTAAGAG GTAATGAAAA GGAAGTAAAG 
CTGGGGGTGT GCGGAAGATA CACCCATGGA CGACCTGTTC TGGGAAGAGT AAAGATGACA 
CTCTGCTCAT CCAGAATAAT CAATTCTTAT TTCGGTTCCC ATTATCAAAT TCCATTTTGT 
GTTACTGGGT CAGCAGTACT GAACAAGACT GGGTGCGCCT TTGAGATGTT CCCCTTGTCA 
TCTTTTATTC AAAAATCAGT AGTGGAATAC ATTACATCGC AGTCTCTCGA GTTCACTGCA 
TCCATTACTG AGGAGGGAAC AGGCATCACT GAGGGGGGGA CCGAAAAAGT CACATTGTCA 
TATATTATTG GTACAGTCAA ATTTATTGAC GTGCCTGATT TTTATGAGGA AGGATCAGTC 
ATAAAGGCCA AGGTTAAAGT TGAGCTATTC AATGGACAAC CAGTTTCTAA CATCAAGTTA 
GTTGTCTACG TCCCATCTGG GATTGGAGAA CTAATCACAG ACATCAATGG GCTGGCTAAT 
TTCACCATTG ATACGTCCTT TGGGATAGGT ACTACTTTAA TTTTAACGGC AAGTTTATTA 
AGCACACCAG AGTTTGTTCC ATTCAGTTTT GATTCTGCGC ACAAAACCCT GCAAAAAAAG 
CCCAAACAAG ATTCATTTAA TAAGAGCCAA CTGGATATAA AGGCACTGGA GGAACCGCTG 
AAGTGTGGGG TGGAGATTTC AGTGACCGTG CAGTACACTA TTGCTCGAGA GACTGCTGAA 
AGTGTTACCA TAGTCTATCT GGTTTTATCA AGAGCACAAA TAGTCTCCCA CGGATATGAG 
AGCATTGAGC TGAAAGGCTC TGACAGAGTA GTGAAGGGAG AAGTGACGTT TAAAGTCCCT 
TTTCGCTCTC GGATGGCTCC AGAAATACAG TTTTTGGCAT ACTGTGTGCT GCCCAGTGAG 
AATGTACTTG CTGCGTCCAA AACATTTCAA ACTGAGAAAT GTTTTGAAAA CAAGGTCTCG 
CTGCGGTTTT CACCAACTAC TGCAGTTCCA GGTGAAGAAA ACACTCTCCA GCTCTCAGCT 
CAGCCTGGTT CACTGTGTGG TATCAGTGCT GTAGATCAGA GTGTCCTGAT CCTGGAGTCA 
GGACAACGCC TGGATGCTGA CAAAGTGTTT AAAATGTTGC CAACAATGAC TTTTACATAC 
GATGTTGAAG ATCAGATGGA ATGCTTGAAA TTTAGATCCA AAAGAAGTTT GCGGTTTATG 
CCAGCTATTT CTCAATATGG AGCAGTACCA TCCTACCATA ATATGTACCC CCCACTTTCA 
GGGAGTACAG GAGATGCTGT GTTTGAGACT TTCAAGAACA TGGGATTAAA GGTGGCAACA 
AATTTGGTTA TTAGAATCCC ACATTGTATT AAATTCAGAG ATGTATTGTT TCAGAGAGCA 
TATGGTGGTT ATGGTGTTGG TTTTGGTTAT GGAGCTGTTC CACGTGGAAT AGATCCTCCT 
GCAATTCCGG CTTTAGCAGG AGCACCAGGT GCAGGAGCCC TTATTCCAGC AAGAGGTGGA 
GTCGTAGCTG AAGCTGAAAG ACTTATACCA AGCCCACTTC CAACTTTGAG CATCTCTCTC 
TTGCCTGAGG AGACAATTCG CACATTCTTT CCAGAAACTT GGATCTGGGA AATTGTGGAG 
GTTGGAGACT CTGGATCAGC TGAGGTTCCT GTCACAGTTC CTGACACCAT CACATCTTGG 
GCGACGGAGG CCTTCTGTCT GTCCTCCACA GGTCTGGGTT TGGCTCCTCC TGCTAAGCTG 
ATAGTTTTCC AGCCCTTCTT CCTGGAGCTC TCTCTGCCTT ACTCCATTGT TCGTGGGGAG 
ATCTTTGAGC TCAAGGCCAC TGTCTTCAAC TATCAGTCCA AGTGCATCAT GGTTAAAGTG 
AGTCCAGCTC CTTCCTCAGA CTACACTCTC AAAGCCTCCT CTGATGATCA GTATTCATCC 
TGTCTCTGTG CTAATGGAAG AAAAACCTTT AAATGGATCC TTACTCCTTC TGTTCTTGGA 
GTGGTGAATG TTACAGTCAG TGCAGAGGCA GAGGCGTCCC AGACTGTGTG TGACAATGAG 
ATTGTGAGTG TGCCAGAGAG AGGACGCATT GACACCGTCA CACGGAGTCT GCGTGTCAAT 
GCTGAAGGAA TTGAAAAAAC TAAAAGCCAC AGCTGGCTGC TTTGCCCCAA AGGGCAAAAT 
CACTTGGAGG AGGTAGAGCT GGCTTTTCCA CAAAATGTGA TAGAGGGTTC AGGAAGAGCC 
ACTGTTTCAG TGCTTGGAGA CTTATTGGGC CGTGCTCTGA AGAACCTTGA TGGTTTATTA 
CGAATGCCGT ACGGCTGTGG AGAACAAAAT ATTGCTGTTC TTTCTCCCAA CATTTACATT 
CTGCAGTATC TGGAGAACAC AAATCAGCTC ACATCCGCCA TTCGAGAGAG AGCCTCTGGC 
TTCCTTAAGA GCGGTTATCA GAGGCAACTG AACTATTTGA ATTTGGATGG GTCATATACA 
ACATTTGGTC AAGGTGAAGG CAACACATGG CTGACCGCGT TTGTGCTGAG GACTTTCGGG 
AAAGCACAAG ATTACATCTT CATTGACCCC CCAAAAATCG AGAAATCAAA GGAATGGCTG 
GTGAGCCATC AACGACCAGA TGGTTCATAT CAAATCAAGG GAAAACTCTT TAATAACAGA 
ATGAAGGGTG GTGTAAGTGA CAGCGTGACC ATCACTGCCT ATATAACCGC ATCACTACTT 
GAGCTGAACA TACCAGTTAC GGATCCGGCT GTCAGTAAAG GCCTTTCACA CTTAAAGCAG 
TATGCTGGTG ATATTCGTAA CCTTTACACC TCTGCACTGC TAAGCTATAC TTTCAGTTTG 
GCAAAAGACA AAGAGGTTCG GGACAGCCTT TTGAACAAGC TTAAGAACAT TGCGATTTCA 
GAAGGTCCTC TTGTCCACTG GTCTCAGTCT GCATCTGCTG ATGACTCTGA TTCTCTGGAT 
GTGGAGATCA GCTCATATGT GCTGCTAGCT GTTCTCACTG CAGATTCACT CACTACAGCT 
GATCTGGGCT TTGCTAACAG GATTGTCAGC TGGCTTGTGA AGCAGCAGAA TGCCTATGGA 
GGATTCTCCT CCACGCAGGA CACAGTGGTG GCTCTTCAGG CTCTGTCTTT GTACGCCACC 
AAAGTGTTCA GCTCTGACGG CTCCAGCACA GTGACTGTAC AGTCAGCAGG AGACTCTCAC 
CACTTTGATG TCAATCAGGA CAACAAGTTA CTGTACCAGG AGAAGCAGCT GGCCAATGTG 
CCGGGCAAAT ACAGCATTGA AGTCAAGGGC TCAGCCTGTG TGTCTGTGCA GGTCTGTGAA 
GTGCAGCAAA ATTTAAAACA CGGATATAAG GGAATACAAT ATATAGAGAC CTCCAACATG 
ATCATTGTAG ATATGAAACT CTTGTCTGGT TTCAGCGCAG AACCGATTTT TATGGATGAG 
GTAAAGAAAG CAGTAAGTCG TGTTGAATCC AGTGATGAGC ATATCATCGT ATATCTAAAC 
GAGATTCGGA ATAACATAAA CTACCGGCTT CCGTTTCGTA TTAAAAGGAT CTTCTCTGTT 
AAAAACCTCA AGCCAGCAGT TATAAAAGTC TACGACTACT ACCAGACCAG TGAGCGAGCT 
GAAGCAGAAT ACACAAATCA CTGTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289985.1
CDS	45..4394
Translation	MMALIHKRGLLITCFLLFVSQGTSKPNFMVTFPAIIRSGSEAKLCMSLLKPEKNLQLTITLVSSNQNRTLLQEKTEKEFHRCLDFQAPESASVQEIYVEAQGKYTHLIEKRKVKFVSHNPLTLIKTDKPIYNPGKTVQFRIFTIDFDFKPVDETYKLIAIQDNRGNRINQWINATSTHGKILQLSHSLNPEASVGLYELSVETNKGKFLEFFQVKEYVLPKFEVKIHKPKLVRGNEKEVKLGVCGRYTHGRPVLGRVKMTLCSSRIINSYFGSHYQIPFCVTGSAVLNKTGCAFEMFPLSSFIQKSVVEYITSQSLEFTASITEEGTGITEGGTEKVTLSYIIGTVKFIDVPDFYEEGSVIKAKVKVELFNGQPVSNIKLVVYVPSGIGELITDINGLANFTIDTSFGIGTTLILTASLLSTPEFVPFSFDSAHKTLQKKPKQDSFNKSQLDIKALEEPLKCGVEISVTVQYTIARETAESVTIVYLVLSRAQIVSHGYESIELKGSDRVVKGEVTFKVPFRSRMAPEIQFLAYCVLPSENVLAASKTFQTEKCFENKVSLRFSPTTAVPGEENTLQLSAQPGSLCGISAVDQSVLILESGQRLDADKVFKMLPTMTFTYDVEDQMECLKFRSKRSLRFMPAISQYGAVPSYHNMYPPLSGSTGDAVFETFKNMGLKVATNLVIRIPHCIKFRDVLFQRAYGGYGVGFGYGAVPRGIDPPAIPALAGAPGAGALIPARGGVVAEAERLIPSPLPTLSISLLPEETIRTFFPETWIWEIVEVGDSGSAEVPVTVPDTITSWATEAFCLSSTGLGLAPPAKLIVFQPFFLELSLPYSIVRGEIFELKATVFNYQSKCIMVKVSPAPSSDYTLKASSDDQYSSCLCANGRKTFKWILTPSVLGVVNVTVSAEAEASQTVCDNEIVSVPERGRIDTVTRSLRVNAEGIEKTKSHSWLLCPKGQNHLEEVELAFPQNVIEGSGRATVSVLGDLLGRALKNLDGLLRMPYGCGEQNIAVLSPNIYILQYLENTNQLTSAIRERASGFLKSGYQRQLNYLNLDGSYTTFGQGEGNTWLTAFVLRTFGKAQDYIFIDPPKIEKSKEWLVSHQRPDGSYQIKGKLFNNRMKGGVSDSVTITAYITASLLELNIPVTDPAVSKGLSHLKQYAGDIRNLYTSALLSYTFSLAKDKEVRDSLLNKLKNIAISEGPLVHWSQSASADDSDSLDVEISSYVLLAVLTADSLTTADLGFANRIVSWLVKQQNAYGGFSSTQDTVVALQALSLYATKVFSSDGSSTVTVQSAGDSHHFDVNQDNKLLYQEKQLANVPGKYSIEVKGSACVSVQVCEVQQNLKHGYKGIQYIETSNMIIVDMKLLSGFSAEPIFMDEVKKAVSRVESSDEHIIVYLNEIRNNINYRLPFRIKRIFSVKNLKPAVIKVYDYYQTSERAEAEYTNHCA

Target ORF information:

RefSeq Version	XM_009291710.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio alpha-2-macroglobulin-like protein 1 (LOC569025), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291710.3

ORF Insert Sequence:

ATGATGGCTT TGATTCACAA AAGAGGACTA TTAATAACAT GTTTTTTGTT GTTTGTCAGT 
CAAGGAACAT CTAAACCGAA TTTTATGGTG ACATTTCCAG CCATTATAAG ATCAGGCTCA 
GAGGCTAAAT TATGTATGAG CCTTCTGAAA CCAGAAAAAA ATCTGCAGCT TACCATTACT 
CTGGTTAGCA GTAACCAGAA CCGAACACTT CTGCAGGAGA AAACAGAGAA GGAATTCCAT 
CGCTGCCTTG ACTTTCAAGC TCCTGAGTCA GCGTCTGTGC AGGAGATTTA TGTGGAAGCT 
CAAGGGAAAT ACACACATCT GATCGAAAAG AGAAAAGTCA AGTTTGTTTC ACATAATCCA 
CTGACTCTAA TAAAAACTGA TAAACCAATC TACAATCCTG GAAAAACAGT TCAATTCAGG 
ATTTTCACAA TTGACTTTGA CTTTAAACCA GTTGATGAAA CCTACAAACT GATAGCAATC 
CAGGACAATC GAGGGAACAG AATTAATCAG TGGATTAATG CCACATCCAC CCATGGCAAA 
ATTCTGCAGC TTTCCCATTC ATTGAACCCA GAGGCTTCTG TTGGTTTATA TGAGCTGTCA 
GTTGAAACCA ACAAGGGAAA ATTTCTAGAA TTTTTTCAGG TTAAAGAATA TGTTCTACCG 
AAATTCGAAG TGAAAATACA TAAGCCAAAG TTAGTAAGAG GTAATGAAAA GGAAGTAAAG 
CTGGGGGTGT GCGGAAGATA CACCCATGGA CGACCTGTTC TGGGAAGAGT AAAGATGACA 
CTCTGCTCAT CCAGAATAAT CAATTCTTAT TTCGGTTCCC ATTATCAAAT TCCATTTTGT 
GTTACTGGGT CAGCAGTACT GAACAAGACT GGGTGCGCCT TTGAGATGTT CCCCTTGTCA 
TCTTTTATTC AAAAATCAGT AGTGGAATAC ATTACATCGC AGTCTCTCGA GTTCACTGCA 
TCCATTACTG AGGAGGGAAC AGGCATCACT GAGGGGGGGA CCGAAAAAGT CACATTGTCA 
TATATTATTG GTACAGTCAA ATTTATTGAC GTGCCTGATT TTTATGAGGA AGGATCAGTC 
ATAAAGGCCA AGGTTAAAGT TGAGCTATTC AATGGACAAC CAGTTTCTAA CATCAAGTTA 
GTTGTCTACG TCCCATCTGG GATTGGAGAA CTAATCACAG ACATCAATGG GCTGGCTAAT 
TTCACCATTG ATACGTCCTT TGGGATAGGT ACTACTTTAA TTTTAACGGC AAGTTTATTA 
AGCACACCAG AGTTTGTTCC ATTCAGTTTT GATTCTGCGC ACAAAACCCT GCAAAAAAAG 
CCCAAACAAG ATTCATTTAA TAAGAGCCAA CTGGATATAA AGGCACTGGA GGAACCGCTG 
AAGTGTGGGG TGGAGATTTC AGTGACCGTG CAGTACACTA TTGCTCGAGA GACTGCTGAA 
AGTGTTACCA TAGTCTATCT GGTTTTATCA AGAGCACAAA TAGTCTCCCA CGGATATGAG 
AGCATTGAGC TGAAAGGCTC TGACAGAGTA GTGAAGGGAG AAGTGACGTT TAAAGTCCCT 
TTTCGCTCTC GGATGGCTCC AGAAATACAG TTTTTGGCAT ACTGTGTGCT GCCCAGTGAG 
AATGTACTTG CTGCGTCCAA AACATTTCAA ACTGAGAAAT GTTTTGAAAA CAAGGTCTCG 
CTGCGGTTTT CACCAACTAC TGCAGTTCCA GGTGAAGAAA ACACTCTCCA GCTCTCAGCT 
CAGCCTGGTT CACTGTGTGG TATCAGTGCT GTAGATCAGA GTGTCCTGAT CCTGGAGTCA 
GGACAACGCC TGGATGCTGA CAAAGTGTTT AAAATGTTGC CAACAATGAC TTTTACATAC 
GATGTTGAAG ATCAGATGGA ATGCTTGAAA TTTAGATCCA AAAGAAGTTT GCGGTTTATG 
CCAGCTATTT CTCAATATGG AGCAGTACCA TCCTACCATA ATATGTACCC CCCACTTTCA 
GGGAGTACAG GAGATGCTGT GTTTGAGACT TTCAAGAACA TGGGATTAAA GGTGGCAACA 
AATTTGGTTA TTAGAATCCC ACATTGTATT AAATTCAGAG ATGTATTGTT TCAGAGAGCA 
TATGGTGGTT ATGGTGTTGG TTTTGGTTAT GGAGCTGTTC CACGTGGAAT AGATCCTCCT 
GCAATTCCGG CTTTAGCAGG AGCACCAGGT GCAGGAGCCC TTATTCCAGC AAGAGGTGGA 
GTCGTAGCTG AAGCTGAAAG ACTTATACCA AGCCCACTTC CAACTTTGAG CATCTCTCTC 
TTGCCTGAGG AGACAATTCG CACATTCTTT CCAGAAACTT GGATCTGGGA AATTGTGGAG 
GTTGGAGACT CTGGATCAGC TGAGGTTCCT GTCACAGTTC CTGACACCAT CACATCTTGG 
GCGACGGAGG CCTTCTGTCT GTCCTCCACA GGTCTGGGTT TGGCTCCTCC TGCTAAGCTG 
ATAGTTTTCC AGCCCTTCTT CCTGGAGCTC TCTCTGCCTT ACTCCATTGT TCGTGGGGAG 
ATCTTTGAGC TCAAGGCCAC TGTCTTCAAC TATCAGTCCA AGTGCATCAT GGTTAAAGTG 
AGTCCAGCTC CTTCCTCAGA CTACACTCTC AAAGCCTCCT CTGATGATCA GTATTCATCC 
TGTCTCTGTG CTAATGGAAG AAAAACCTTT AAATGGATCC TTACTCCTTC TGTTCTTGGA 
GTGGTGAATG TTACAGTCAG TGCAGAGGCA GAGGCGTCCC AGACTGTGTG TGACAATGAG 
ATTGTGAGTG TGCCAGAGAG AGGACGCATT GACACCGTCA CACGGAGTCT GCGTGTCAAT 
GCTGAAGGAA TTGAAAAAAC TAAAAGCCAC AGCTGGCTGC TTTGCCCCAA AGGGCAAAAT 
CACTTGGAGG AGGTAGAGCT GGCTTTTCCA CAAAATGTGA TAGAGGGTTC AGGAAGAGCC 
ACTGTTTCAG TGCTTGGAGA CTTATTGGGC CGTGCTCTGA AGAACCTTGA TGGTTTATTA 
CGAATGCCGT ACGGCTGTGG AGAACAAAAT ATTGCTGTTC TTTCTCCCAA CATTTACATT 
CTGCAGTATC TGGAGAACAC AAATCAGCTC ACATCCGCCA TTCGAGAGAG AGCCTCTGGC 
TTCCTTAAGA GCGGTTATCA GAGGCAACTG AACTATTTGA ATTTGGATGG GTCATATACA 
ACATTTGGTC AAGGTGAAGG CAACACATGG CTGACCGCGT TTGTGCTGAG GACTTTCGGG 
AAAGCACAAG ATTACATCTT CATTGACCCC CCAAAAATCG AGAAATCAAA GGAATGGCTG 
GTGAGCCATC AACGACCAGA TGGTTCATAT CAAATCAAGG GAAAACTCTT TAATAACAGA 
ATGAAGGGTG GTGTAAGTGA CAGCGTGACC ATCACTGCCT ATATAACCGC ATCACTACTT 
GAGCTGAACA TACCAGTTAC GGATCCGGCT GTCAGTAAAG GCCTTTCACA CTTAAAGCAG 
TATGCTGGTG ATATTCGTAA CCTTTACACC TCTGCACTGC TAAGCTATAC TTTCAGTTTG 
GCAAAAGACA AAGAGGTTCG GGACAGCCTT TTGAACAAGC TTAAGAACAT TGCGATTTCA 
GAAGGTCCTC TTGTCCACTG GTCTCAGTCT GCATCTGCTG ATGACTCTGA TTCTCTGGAT 
GTGGAGATCA GCTCATATGT GCTGCTAGCT GTTCTCACTG CAGATTCACT CACTACAGCT 
GATCTGGGCT TTGCTAACAG GATTGTCAGC TGGCTTGTGA AGCAGCAGAA TGCCTATGGA 
GGATTCTCCT CCACGCAGGA CACAGTGGTG GCTCTTCAGG CTCTGTCTTT GTACGCCACC 
AAAGTGTTCA GCTCTGACGG CTCCAGCACA GTGACTGTAC AGTCAGCAGG AGACTCTCAC 
CACTTTGATG TCAATCAGGA CAACAAGTTA CTGTACCAGG AGAAGCAGCT GGCCAATGTG 
CCGGGCAAAT ACAGCATTGA AGTCAAGGGC TCAGCCTGTG TGTCTGTGCA GGTCTGTGAA 
GTGCAGCAAA ATTTAAAACA CGGATATAAG GGAATACAAT ATATAGAGAC CTCCAACATG 
ATCATTGTAG ATATGAAACT CTTGTCTGGT TTCAGCGCAG AACCGATTTT TATGGATGAG 
GTAAAGAAAG CAGTAAGTCG TGTTGAATCC AGTGATGAGC ATATCATCGT ATATCTAAAC 
GAGATTCGGA ATAACATAAA CTACCGGCTT CCGTTTCGTA TTAAAAGGAT CTTCTCTGTT 
AAAAACCTCA AGCCAGCAGT TATAAAAGTC TACGACTACT ACCAGACCAG TGAGCGAGCT 
GAAGCAGAAT ACACAAATCA CTGTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC569025 ( XM_009291710.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009289985.1 Danio rerio alpha-2-macroglobulin-like protein 1 (LOC569025), transcript variant X2, mRNA.

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CloneID	ODa45635
Clone ID Related Accession (Same CDS sequence)	XM_009291710.3 , XM_009291710.2
Accession Version	XM_009291710.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	alpha-2-macroglobulin-like protein 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291710.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTT TGATTCACAA AAGAGGACTA TTAATAACAT GTTTTTTGTT GTTTGTCAGT 
CAAGGAACAT CTAAACCGAA TTTTATGGTG ACATTTCCAG CCATTATAAG ATCAGGCTCA 
GAGGCTAAAT TATGTATGAG CCTTCTGAAA CCAGAAAAAA ATCTGCAGCT TACCATTACT 
CTGGTTAGCA GTAACCAGAA CCGAACACTT CTGCAGGAGA AAACAGAGAA GGAATTCCAT 
CGCTGCCTTG ACTTTCAAGC TCCTGAGTCA GCGTCTGTGC AGGAGATTTA TGTGGAAGCT 
CAAGGGAAAT ACACACATCT GATCGAAAAG AGAAAAGTCA AGTTTGTTTC ACATAATCCA 
CTGACTCTAA TAAAAACTGA TAAACCAATC TACAATCCTG GAAAAACAGT TCAATTCAGG 
ATTTTCACAA TTGACTTTGA CTTTAAACCA GTTGATGAAA CCTACAAACT GATAGCAATC 
CAGGACAATC GAGGGAACAG AATTAATCAG TGGATTAATG CCACATCCAC CCATGGCAAA 
ATTCTGCAGC TTTCCCATTC ATTGAACCCA GAGGCTTCTG TTGGTTTATA TGAGCTGTCA 
GTTGAAACCA ACAAGGGAAA ATTTCTAGAA TTTTTTCAGG TTAAAGAATA TGTTCTACCG 
AAATTCGAAG TGAAAATACA TAAGCCAAAG TTAGTAAGAG GTAATGAAAA GGAAGTAAAG 
CTGGGGGTGT GCGGAAGATA CACCCATGGA CGACCTGTTC TGGGAAGAGT AAAGATGACA 
CTCTGCTCAT CCAGAATAAT CAATTCTTAT TTCGGTTCCC ATTATCAAAT TCCATTTTGT 
GTTACTGGGT CAGCAGTACT GAACAAGACT GGGTGCGCCT TTGAGATGTT CCCCTTGTCA 
TCTTTTATTC AAAAATCAGT AGTGGAATAC ATTACATCGC AGTCTCTCGA GTTCACTGCA 
TCCATTACTG AGGAGGGAAC AGGCATCACT GAGGGGGGGA CCGAAAAAGT CACATTGTCA 
TATATTATTG GTACAGTCAA ATTTATTGAC GTGCCTGATT TTTATGAGGA AGGATCAGTC 
ATAAAGGCCA AGGTTAAAGT TGAGCTATTC AATGGACAAC CAGTTTCTAA CATCAAGTTA 
GTTGTCTACG TCCCATCTGG GATTGGAGAA CTAATCACAG ACATCAATGG GCTGGCTAAT 
TTCACCATTG ATACGTCCTT TGGGATAGGT ACTACTTTAA TTTTAACGGC AAGTTTATTA 
AGCACACCAG AGTTTGTTCC ATTCAGTTTT GATTCTGCGC ACAAAACCCT GCAAAAAAAG 
CCCAAACAAG ATTCATTTAA TAAGAGCCAA CTGGATATAA AGGCACTGGA GGAACCGCTG 
AAGTGTGGGG TGGAGATTTC AGTGACCGTG CAGTACACTA TTGCTCGAGA GACTGCTGAA 
AGTGTTACCA TAGTCTATCT GGTTTTATCA AGAGCACAAA TAGTCTCCCA CGGATATGAG 
AGCATTGAGC TGAAAGGCTC TGACAGAGTA GTGAAGGGAG AAGTGACGTT TAAAGTCCCT 
TTTCGCTCTC GGATGGCTCC AGAAATACAG TTTTTGGCAT ACTGTGTGCT GCCCAGTGAG 
AATGTACTTG CTGCGTCCAA AACATTTCAA ACTGAGAAAT GTTTTGAAAA CAAGGTCTCG 
CTGCGGTTTT CACCAACTAC TGCAGTTCCA GGTGAAGAAA ACACTCTCCA GCTCTCAGCT 
CAGCCTGGTT CACTGTGTGG TATCAGTGCT GTAGATCAGA GTGTCCTGAT CCTGGAGTCA 
GGACAACGCC TGGATGCTGA CAAAGTGTTT AAAATGTTGC CAACAATGAC TTTTACATAC 
GATGTTGAAG ATCAGATGGA ATGCTTGAAA TTTAGATCCA AAAGAAGTTT GCGGTTTATG 
CCAGCTATTT CTCAATATGG AGCAGTACCA TCCTACCATA ATATGTACCC CCCACTTTCA 
GGGAGTACAG GAGATGCTGT GTTTGAGACT TTCAAGAACA TGGGATTAAA GGTGGCAACA 
AATTTGGTTA TTAGAATCCC ACATTGTATT AAATTCAGAG ATGTATTGTT TCAGAGAGCA 
TATGGTGGTT ATGGTGTTGG TTTTGGTTAT GGAGCTGTTC CACGTGGAAT AGATCCTCCT 
GCAATTCCGG CTTTAGCAGG AGCACCAGGT GCAGGAGCCC TTATTCCAGC AAGAGGTGGA 
GTCGTAGCTG AAGCTGAAAG ACTTATACCA AGCCCACTTC CAACTTTGAG CATCTCTCTC 
TTGCCTGAGG AGACAATTCG CACATTCTTT CCAGAAACTT GGATCTGGGA AATTGTGGAG 
GTTGGAGACT CTGGATCAGC TGAGGTTCCT GTCACAGTTC CTGACACCAT CACATCTTGG 
GCGACGGAGG CCTTCTGTCT GTCCTCCACA GGTCTGGGTT TGGCTCCTCC TGCTAAGCTG 
ATAGTTTTCC AGCCCTTCTT CCTGGAGCTC TCTCTGCCTT ACTCCATTGT TCGTGGGGAG 
ATCTTTGAGC TCAAGGCCAC TGTCTTCAAC TATCAGTCCA AGTGCATCAT GGTTAAAGTG 
AGTCCAGCTC CTTCCTCAGA CTACACTCTC AAAGCCTCCT CTGATGATCA GTATTCATCC 
TGTCTCTGTG CTAATGGAAG AAAAACCTTT AAATGGATCC TTACTCCTTC TGTTCTTGGA 
GTGGTGAATG TTACAGTCAG TGCAGAGGCA GAGGCGTCCC AGACTGTGTG TGACAATGAG 
ATTGTGAGTG TGCCAGAGAG AGGACGCATT GACACCGTCA CACGGAGTCT GCGTGTCAAT 
GCTGAAGGAA TTGAAAAAAC TAAAAGCCAC AGCTGGCTGC TTTGCCCCAA AGGGCAAAAT 
CACTTGGAGG AGGTAGAGCT GGCTTTTCCA CAAAATGTGA TAGAGGGTTC AGGAAGAGCC 
ACTGTTTCAG TGCTTGGAGA CTTATTGGGC CGTGCTCTGA AGAACCTTGA TGGTTTATTA 
CGAATGCCGT ACGGCTGTGG AGAACAAAAT ATTGCTGTTC TTTCTCCCAA CATTTACATT 
CTGCAGTATC TGGAGAACAC AAATCAGCTC ACATCCGCCA TTCGAGAGAG AGCCTCTGGC 
TTCCTTAAGA GCGGTTATCA GAGGCAACTG AACTATTTGA ATTTGGATGG GTCATATACA 
ACATTTGGTC AAGGTGAAGG CAACACATGG CTGACCGCGT TTGTGCTGAG GACTTTCGGG 
AAAGCACAAG ATTACATCTT CATTGACCCC CCAAAAATCG AGAAATCAAA GGAATGGCTG 
GTGAGCCATC AACGACCAGA TGGTTCATAT CAAATCAAGG GAAAACTCTT TAATAACAGA 
ATGAAGGGTG GTGTAAGTGA CAGCGTGACC ATCACTGCCT ATATAACCGC ATCACTACTT 
GAGCTGAACA TACCAGTTAC GGATCCGGCT GTCAGTAAAG GCCTTTCACA CTTAAAGCAG 
TATGCTGGTG ATATTCGTAA CCTTTACACC TCTGCACTGC TAAGCTATAC TTTCAGTTTG 
GCAAAAGACA AAGAGGTTCG GGACAGCCTT TTGAACAAGC TTAAGAACAT TGCGATTTCA 
GAAGGTCCTC TTGTCCACTG GTCTCAGTCT GCATCTGCTG ATGACTCTGA TTCTCTGGAT 
GTGGAGATCA GCTCATATGT GCTGCTAGCT GTTCTCACTG CAGATTCACT CACTACAGCT 
GATCTGGGCT TTGCTAACAG GATTGTCAGC TGGCTTGTGA AGCAGCAGAA TGCCTATGGA 
GGATTCTCCT CCACGCAGGA CACAGTGGTG GCTCTTCAGG CTCTGTCTTT GTACGCCACC 
AAAGTGTTCA GCTCTGACGG CTCCAGCACA GTGACTGTAC AGTCAGCAGG AGACTCTCAC 
CACTTTGATG TCAATCAGGA CAACAAGTTA CTGTACCAGG AGAAGCAGCT GGCCAATGTG 
CCGGGCAAAT ACAGCATTGA AGTCAAGGGC TCAGCCTGTG TGTCTGTGCA GGTCTGTGAA 
GTGCAGCAAA ATTTAAAACA CGGATATAAG GGAATACAAT ATATAGAGAC CTCCAACATG 
ATCATTGTAG ATATGAAACT CTTGTCTGGT TTCAGCGCAG AACCGATTTT TATGGATGAG 
GTAAAGAAAG CAGTAAGTCG TGTTGAATCC AGTGATGAGC ATATCATCGT ATATCTAAAC 
GAGATTCGGA ATAACATAAA CTACCGGCTT CCGTTTCGTA TTAAAAGGAT CTTCTCTGTT 
AAAAACCTCA AGCCAGCAGT TATAAAAGTC TACGACTACT ACCAGACCAG TGAGCGAGCT 
GAAGCAGAAT ACACAAATCA CTGTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289985.1
CDS	32..4381
Translation	MMALIHKRGLLITCFLLFVSQGTSKPNFMVTFPAIIRSGSEAKLCMSLLKPEKNLQLTITLVSSNQNRTLLQEKTEKEFHRCLDFQAPESASVQEIYVEAQGKYTHLIEKRKVKFVSHNPLTLIKTDKPIYNPGKTVQFRIFTIDFDFKPVDETYKLIAIQDNRGNRINQWINATSTHGKILQLSHSLNPEASVGLYELSVETNKGKFLEFFQVKEYVLPKFEVKIHKPKLVRGNEKEVKLGVCGRYTHGRPVLGRVKMTLCSSRIINSYFGSHYQIPFCVTGSAVLNKTGCAFEMFPLSSFIQKSVVEYITSQSLEFTASITEEGTGITEGGTEKVTLSYIIGTVKFIDVPDFYEEGSVIKAKVKVELFNGQPVSNIKLVVYVPSGIGELITDINGLANFTIDTSFGIGTTLILTASLLSTPEFVPFSFDSAHKTLQKKPKQDSFNKSQLDIKALEEPLKCGVEISVTVQYTIARETAESVTIVYLVLSRAQIVSHGYESIELKGSDRVVKGEVTFKVPFRSRMAPEIQFLAYCVLPSENVLAASKTFQTEKCFENKVSLRFSPTTAVPGEENTLQLSAQPGSLCGISAVDQSVLILESGQRLDADKVFKMLPTMTFTYDVEDQMECLKFRSKRSLRFMPAISQYGAVPSYHNMYPPLSGSTGDAVFETFKNMGLKVATNLVIRIPHCIKFRDVLFQRAYGGYGVGFGYGAVPRGIDPPAIPALAGAPGAGALIPARGGVVAEAERLIPSPLPTLSISLLPEETIRTFFPETWIWEIVEVGDSGSAEVPVTVPDTITSWATEAFCLSSTGLGLAPPAKLIVFQPFFLELSLPYSIVRGEIFELKATVFNYQSKCIMVKVSPAPSSDYTLKASSDDQYSSCLCANGRKTFKWILTPSVLGVVNVTVSAEAEASQTVCDNEIVSVPERGRIDTVTRSLRVNAEGIEKTKSHSWLLCPKGQNHLEEVELAFPQNVIEGSGRATVSVLGDLLGRALKNLDGLLRMPYGCGEQNIAVLSPNIYILQYLENTNQLTSAIRERASGFLKSGYQRQLNYLNLDGSYTTFGQGEGNTWLTAFVLRTFGKAQDYIFIDPPKIEKSKEWLVSHQRPDGSYQIKGKLFNNRMKGGVSDSVTITAYITASLLELNIPVTDPAVSKGLSHLKQYAGDIRNLYTSALLSYTFSLAKDKEVRDSLLNKLKNIAISEGPLVHWSQSASADDSDSLDVEISSYVLLAVLTADSLTTADLGFANRIVSWLVKQQNAYGGFSSTQDTVVALQALSLYATKVFSSDGSSTVTVQSAGDSHHFDVNQDNKLLYQEKQLANVPGKYSIEVKGSACVSVQVCEVQQNLKHGYKGIQYIETSNMIIVDMKLLSGFSAEPIFMDEVKKAVSRVESSDEHIIVYLNEIRNNINYRLPFRIKRIFSVKNLKPAVIKVYDYYQTSERAEAEYTNHCA

Target ORF information:

RefSeq Version	XM_009291710.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio alpha-2-macroglobulin-like protein 1 (LOC569025), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291710.2

ORF Insert Sequence:

ATGATGGCTT TGATTCACAA AAGAGGACTA TTAATAACAT GTTTTTTGTT GTTTGTCAGT 
CAAGGAACAT CTAAACCGAA TTTTATGGTG ACATTTCCAG CCATTATAAG ATCAGGCTCA 
GAGGCTAAAT TATGTATGAG CCTTCTGAAA CCAGAAAAAA ATCTGCAGCT TACCATTACT 
CTGGTTAGCA GTAACCAGAA CCGAACACTT CTGCAGGAGA AAACAGAGAA GGAATTCCAT 
CGCTGCCTTG ACTTTCAAGC TCCTGAGTCA GCGTCTGTGC AGGAGATTTA TGTGGAAGCT 
CAAGGGAAAT ACACACATCT GATCGAAAAG AGAAAAGTCA AGTTTGTTTC ACATAATCCA 
CTGACTCTAA TAAAAACTGA TAAACCAATC TACAATCCTG GAAAAACAGT TCAATTCAGG 
ATTTTCACAA TTGACTTTGA CTTTAAACCA GTTGATGAAA CCTACAAACT GATAGCAATC 
CAGGACAATC GAGGGAACAG AATTAATCAG TGGATTAATG CCACATCCAC CCATGGCAAA 
ATTCTGCAGC TTTCCCATTC ATTGAACCCA GAGGCTTCTG TTGGTTTATA TGAGCTGTCA 
GTTGAAACCA ACAAGGGAAA ATTTCTAGAA TTTTTTCAGG TTAAAGAATA TGTTCTACCG 
AAATTCGAAG TGAAAATACA TAAGCCAAAG TTAGTAAGAG GTAATGAAAA GGAAGTAAAG 
CTGGGGGTGT GCGGAAGATA CACCCATGGA CGACCTGTTC TGGGAAGAGT AAAGATGACA 
CTCTGCTCAT CCAGAATAAT CAATTCTTAT TTCGGTTCCC ATTATCAAAT TCCATTTTGT 
GTTACTGGGT CAGCAGTACT GAACAAGACT GGGTGCGCCT TTGAGATGTT CCCCTTGTCA 
TCTTTTATTC AAAAATCAGT AGTGGAATAC ATTACATCGC AGTCTCTCGA GTTCACTGCA 
TCCATTACTG AGGAGGGAAC AGGCATCACT GAGGGGGGGA CCGAAAAAGT CACATTGTCA 
TATATTATTG GTACAGTCAA ATTTATTGAC GTGCCTGATT TTTATGAGGA AGGATCAGTC 
ATAAAGGCCA AGGTTAAAGT TGAGCTATTC AATGGACAAC CAGTTTCTAA CATCAAGTTA 
GTTGTCTACG TCCCATCTGG GATTGGAGAA CTAATCACAG ACATCAATGG GCTGGCTAAT 
TTCACCATTG ATACGTCCTT TGGGATAGGT ACTACTTTAA TTTTAACGGC AAGTTTATTA 
AGCACACCAG AGTTTGTTCC ATTCAGTTTT GATTCTGCGC ACAAAACCCT GCAAAAAAAG 
CCCAAACAAG ATTCATTTAA TAAGAGCCAA CTGGATATAA AGGCACTGGA GGAACCGCTG 
AAGTGTGGGG TGGAGATTTC AGTGACCGTG CAGTACACTA TTGCTCGAGA GACTGCTGAA 
AGTGTTACCA TAGTCTATCT GGTTTTATCA AGAGCACAAA TAGTCTCCCA CGGATATGAG 
AGCATTGAGC TGAAAGGCTC TGACAGAGTA GTGAAGGGAG AAGTGACGTT TAAAGTCCCT 
TTTCGCTCTC GGATGGCTCC AGAAATACAG TTTTTGGCAT ACTGTGTGCT GCCCAGTGAG 
AATGTACTTG CTGCGTCCAA AACATTTCAA ACTGAGAAAT GTTTTGAAAA CAAGGTCTCG 
CTGCGGTTTT CACCAACTAC TGCAGTTCCA GGTGAAGAAA ACACTCTCCA GCTCTCAGCT 
CAGCCTGGTT CACTGTGTGG TATCAGTGCT GTAGATCAGA GTGTCCTGAT CCTGGAGTCA 
GGACAACGCC TGGATGCTGA CAAAGTGTTT AAAATGTTGC CAACAATGAC TTTTACATAC 
GATGTTGAAG ATCAGATGGA ATGCTTGAAA TTTAGATCCA AAAGAAGTTT GCGGTTTATG 
CCAGCTATTT CTCAATATGG AGCAGTACCA TCCTACCATA ATATGTACCC CCCACTTTCA 
GGGAGTACAG GAGATGCTGT GTTTGAGACT TTCAAGAACA TGGGATTAAA GGTGGCAACA 
AATTTGGTTA TTAGAATCCC ACATTGTATT AAATTCAGAG ATGTATTGTT TCAGAGAGCA 
TATGGTGGTT ATGGTGTTGG TTTTGGTTAT GGAGCTGTTC CACGTGGAAT AGATCCTCCT 
GCAATTCCGG CTTTAGCAGG AGCACCAGGT GCAGGAGCCC TTATTCCAGC AAGAGGTGGA 
GTCGTAGCTG AAGCTGAAAG ACTTATACCA AGCCCACTTC CAACTTTGAG CATCTCTCTC 
TTGCCTGAGG AGACAATTCG CACATTCTTT CCAGAAACTT GGATCTGGGA AATTGTGGAG 
GTTGGAGACT CTGGATCAGC TGAGGTTCCT GTCACAGTTC CTGACACCAT CACATCTTGG 
GCGACGGAGG CCTTCTGTCT GTCCTCCACA GGTCTGGGTT TGGCTCCTCC TGCTAAGCTG 
ATAGTTTTCC AGCCCTTCTT CCTGGAGCTC TCTCTGCCTT ACTCCATTGT TCGTGGGGAG 
ATCTTTGAGC TCAAGGCCAC TGTCTTCAAC TATCAGTCCA AGTGCATCAT GGTTAAAGTG 
AGTCCAGCTC CTTCCTCAGA CTACACTCTC AAAGCCTCCT CTGATGATCA GTATTCATCC 
TGTCTCTGTG CTAATGGAAG AAAAACCTTT AAATGGATCC TTACTCCTTC TGTTCTTGGA 
GTGGTGAATG TTACAGTCAG TGCAGAGGCA GAGGCGTCCC AGACTGTGTG TGACAATGAG 
ATTGTGAGTG TGCCAGAGAG AGGACGCATT GACACCGTCA CACGGAGTCT GCGTGTCAAT 
GCTGAAGGAA TTGAAAAAAC TAAAAGCCAC AGCTGGCTGC TTTGCCCCAA AGGGCAAAAT 
CACTTGGAGG AGGTAGAGCT GGCTTTTCCA CAAAATGTGA TAGAGGGTTC AGGAAGAGCC 
ACTGTTTCAG TGCTTGGAGA CTTATTGGGC CGTGCTCTGA AGAACCTTGA TGGTTTATTA 
CGAATGCCGT ACGGCTGTGG AGAACAAAAT ATTGCTGTTC TTTCTCCCAA CATTTACATT 
CTGCAGTATC TGGAGAACAC AAATCAGCTC ACATCCGCCA TTCGAGAGAG AGCCTCTGGC 
TTCCTTAAGA GCGGTTATCA GAGGCAACTG AACTATTTGA ATTTGGATGG GTCATATACA 
ACATTTGGTC AAGGTGAAGG CAACACATGG CTGACCGCGT TTGTGCTGAG GACTTTCGGG 
AAAGCACAAG ATTACATCTT CATTGACCCC CCAAAAATCG AGAAATCAAA GGAATGGCTG 
GTGAGCCATC AACGACCAGA TGGTTCATAT CAAATCAAGG GAAAACTCTT TAATAACAGA 
ATGAAGGGTG GTGTAAGTGA CAGCGTGACC ATCACTGCCT ATATAACCGC ATCACTACTT 
GAGCTGAACA TACCAGTTAC GGATCCGGCT GTCAGTAAAG GCCTTTCACA CTTAAAGCAG 
TATGCTGGTG ATATTCGTAA CCTTTACACC TCTGCACTGC TAAGCTATAC TTTCAGTTTG 
GCAAAAGACA AAGAGGTTCG GGACAGCCTT TTGAACAAGC TTAAGAACAT TGCGATTTCA 
GAAGGTCCTC TTGTCCACTG GTCTCAGTCT GCATCTGCTG ATGACTCTGA TTCTCTGGAT 
GTGGAGATCA GCTCATATGT GCTGCTAGCT GTTCTCACTG CAGATTCACT CACTACAGCT 
GATCTGGGCT TTGCTAACAG GATTGTCAGC TGGCTTGTGA AGCAGCAGAA TGCCTATGGA 
GGATTCTCCT CCACGCAGGA CACAGTGGTG GCTCTTCAGG CTCTGTCTTT GTACGCCACC 
AAAGTGTTCA GCTCTGACGG CTCCAGCACA GTGACTGTAC AGTCAGCAGG AGACTCTCAC 
CACTTTGATG TCAATCAGGA CAACAAGTTA CTGTACCAGG AGAAGCAGCT GGCCAATGTG 
CCGGGCAAAT ACAGCATTGA AGTCAAGGGC TCAGCCTGTG TGTCTGTGCA GGTCTGTGAA 
GTGCAGCAAA ATTTAAAACA CGGATATAAG GGAATACAAT ATATAGAGAC CTCCAACATG 
ATCATTGTAG ATATGAAACT CTTGTCTGGT TTCAGCGCAG AACCGATTTT TATGGATGAG 
GTAAAGAAAG CAGTAAGTCG TGTTGAATCC AGTGATGAGC ATATCATCGT ATATCTAAAC 
GAGATTCGGA ATAACATAAA CTACCGGCTT CCGTTTCGTA TTAAAAGGAT CTTCTCTGTT 
AAAAACCTCA AGCCAGCAGT TATAAAAGTC TACGACTACT ACCAGACCAG TGAGCGAGCT 
GAAGCAGAAT ACACAAATCA CTGTGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC569025 ( XM_009291711.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009289986.1 Danio rerio alpha-2-macroglobulin-like protein 1 (LOC569025), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa45636
Clone ID Related Accession (Same CDS sequence)	XM_009291711.3 , XM_009291711.2
Accession Version	XM_009291711.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	alpha-2-macroglobulin-like protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291711.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTT TGATTCACAA AAGAGGACTA TTAATAACAT GTTTTTTGTT GTTTGTCAGT 
CAAGGAACAT CTAAACCGAA TTTTATGGTG ACATTTCCAG CCATTATAAG ATCAGGCTCA 
GAGGCTAAAT TATGTATGAG CCTTCTGAAA CCAGAAAAAA ATCTGCAGCT TACCATTACT 
CTGGTTAGCA GTAACCAGAA CCGAACACTT CTGCAGGAGA AAACAGAGAA GGAATTCCAT 
CGCTGCCTTG ACTTTCAAGC TCCTGAGTCA GCGTCTGTGC AGGAGATTTA TGTGGAAGCT 
CAAGGGAAAT ACACACATCT GATCGAAAAG AGAAAAGTCA AGTTTGTTTC ACATAATCCA 
CTGACTCTAA TAAAAACTGA TAAACCAATC TACAATCCTG GAAAAACAGT TCAATTCAGG 
ATTTTCACAA TTGACTTTGA CTTTAAACCA GTTGATGAAA CCTACAAACT GATAGCAATC 
CAGGACAATC GAGGGAACAG AATTAATCAG TGGATTAATG CCACATCCAC CCATGGCAAA 
ATTCTGCAGC TTTCCCATTC ATTGAACCCA GAGGCTTCTG TTGGTTTATA TGAGCTGTCA 
GTTGAAACCA ACAAGGGAAA ATTTCTAGAA TTTTTTCAGG TTAAAGAATA TGTTCTACCG 
AAATTCGAAG TGAAAATACA TAAGCCAAAG TTAGTAAGAG GTAATGAAAA GGAAGTAAAG 
CTGGGGGTGT GCGGAAGATA CACCCATGGA CGACCTGTTC TGGGAAGAGT AAAGATGACA 
CTCTGCTCAT CCAGAATAAT CAATTCTTAT TTCGGTTCCC ATTATCAAAT TCCATTTTGT 
GTTACTGGGT CAGCAGTACT GAACAAGACT GGGTGCGCCT TTGAGATGTT CCCCTTGTCA 
TCTTTTATTC AAAAATCAGT AGTGGAATAC ATTACATCGC AGTCTCTCGA GTTCACTGCA 
TCCATTACTG AGGAGGGAAC AGGCATCACT GAGGGGGGGA CCGAAAAAGT CACATTGTCA 
TATATTATTG GTACAGTCAA ATTTATTGAC GTGCCTGATT TTTATGAGGA AGGATCAGTC 
ATAAAGGCCA AGGTTAAAGT TGAGCTATTC AATGGACAAC CAGTTTCTAA CATCAAGTTA 
GTTGTCTACG TCCCATCTGG GATTGGAGAA CTAATCACAG ACATCAATGG GCTGGCTAAT 
TTCACCATTG ATACGTCCTT TGGGATAGGT ACTACTTTAA TTTTAACGGC AAGTTTATTA 
AGCACACCAG AGTTTGTTCC ATTCAGTTTT GATTCTGCGC ACAAAACCCT GCAAAAAAAG 
CCCAAACAAG ATTCATTTAA TAAGAGCCAA CTGGATATAA AGGCACTGGA GGAACCGCTG 
AAGTGTGGGG TGGAGATTTC AGTGACCGTG CAGTACACTA TTGCTCGAGA GACTGCTGAA 
AGTGTTACCA TAGTCTATCT GGTTTTATCA AGAGCACAAA TAGTCTCCCA CGGATATGAG 
AGCATTGAGC TGAAAGGCTC TGACAGAGTA GTGAAGGGAG AAGTGACGTT TAAAGTCCCT 
TTTCGCTCTC GGATGGCTCC AGAAATACAG TTTTTGGCAT ACTGTGTGCT GCCCAGTGAG 
AATGTACTTG CTGCGTCCAA AACATTTCAA ACTGAGAAAT GTTTTGAAAA CAAGGTCTCG 
CTGCGGTTTT CACCAACTAC TGCAGTTCCA GGTGAAGAAA ACACTCTCCA GCTCTCAGCT 
CAGCCTGGTT CACTGTGTGG TATCAGTGCT GTAGATCAGA GTGTCCTGAT CCTGGAGTCA 
GGACAACGCC TGGATGCTGA CAAAGTGTTT AAAATGTTGC CAACAATGAC TTTTACATAC 
GATGTTGAAG ATCAGATGGA ATGCTTGAAA TTTAGATCCA AAAGAAGTTT GCGGTTTATG 
CCAGCTATTT CTCAATATGG AGCAGTACCA TCCTACCATA ATATGTACCC CCCACTTTCA 
GGGAGTACAG GAGATGCTGT GTTTGAGACT TTCAAGAACA TGGGATTAAA GGTGGCAACA 
AATTTGGTTA TTAGAATCCC ACATTGTATT AAATTCAGAG ATGTATTGTT TCAGAGAGCA 
TATGGTGGTT ATGGTGTTGG TTTTGGTTAT GGAGCTGTTC CACGTGGAAT AGATCCTCCT 
GCAATTCCGG CTTTAGCAGG AGCACCAGGT GCAGGAGCCC TTATTCCAGC AAGAGGTGGA 
GTCGTAGCTG AAGCTGAAAG ACTTATACCA AGCCCACTTC CAACTTTGAG CATCTCTCTC 
TTGCCTGAGG AGACAATTCG CACATTCTTT CCAGAAACTT GGATCTGGGA AATTGTGGAG 
GTTGGAGACT CTGGATCAGC TGAGGTTCCT GTCACAGTTC CTGACACCAT CACATCTTGG 
GCGACGGAGG CCTTCTGTCT GTCCTCCACA GGTCTGGGTT TGGCTCCTCC TGCTAAGCTG 
ATAGTTTTCC AGCCCTTCTT CCTGGAGCTC TCTCTGCCTT ACTCCATTGT TCGTGGGGAG 
ATCTTTGAGC TCAAGGCCAC TGTCTTCAAC TATCAGTCCA AGTGCATCAT GGTTAAAGTG 
AGTCCAGCTC CTTCCTCAGA CTACACTCTC AAAGCCTCCT CTGATGATCA GTATTCATCC 
TGTCTCTGTG CTAATGGAAG AAAAACCTTT AAATGGATCC TTACTCCTTC TGTTCTTGGA 
GTGGTGAATG TTACAGTCAG TGCAGAGGCA GAGGCGTCCC AGACTGTGTG TGACAATGAG 
ATTGTGAGTG TGCCAGAGAG AGGACGCATT GACACCGTCA CACGGAGTCT GCGTGTCAAT 
GCTGAAGGAA TTGAAAAAAC TAAAAGCCAC AGCTGGCTGC TTTGCCCCAA AGGGCAAAAT 
CACTTGGAGG AGGTAGAGCT GGCTTTTCCA CAAAATGTGA TAGAGGGTTC AGGAAGAGCC 
ACTGTTTCAG TGCTTGGAGA CTTATTGGGC CGTGCTCTGA AGAACCTTGA TGGTTTATTA 
CGAATGCCGT ACGGCTGTGG AGAACAAAAT ATTGCTGTTC TTTCTCCCAA CATTTACATT 
CTGCAGTATC TGGAGAACAC AAATCAGCTC ACATCCGCCA TTCGAGAGAG AGCCTCTGGC 
TTCCTTAAGA GCGGTTATCA GAGGCAACTG AACTATTTGA ATTTGGATGG GTCATATACA 
ACATTTGGTC AAGGTGAAGG CAACACATGG CTGACCGCGT TTGTGCTGAG GACTTTCGGG 
AAAGCACAAG ATTACATCTT CATTGACCCC CCAAAAATCG AGAAATCAAA GGAATGGCTG 
GTGAGCCATC AACGACCAGA TGGTTCATAT CAAATCAAGG GAAAACTCTT TAATAACAGA 
ATGAAGGGTG GTGTAAGTGA CAGCGTGACC ATCACTGCCT ATATAACCGC ATCACTACTT 
GAGCTGAACA TACCAGTTAC GGATCCGGCT GTCAGTAAAG GCCTTTCACA CTTAAAGCAG 
TATGCTGGTG ATATTCGTAA CCTTTACACC TCTGCACTGC TAAGCTATAC TTTCAGTTTG 
GCAAAAGACA AAGAGGTTCG GGACAGCCTT TTGAACAAGC TTAAGAACAT TGCGATTTCA 
GAAGGTCCTC TTGTCCACTG GTCTCAGTCT GCATCTGCTG ATGACTCTGA TTCTCTGGAT 
GTGGAGATCA GCTCATATGT GCTGCTAGCT GTTCTCACTG CAGATTCACT CACTACAGCT 
GATCTGGGCT TTGCTAACAG GATTGTCAGC TGGCTTGTGA AGCAGCAGAA TGCCTATGGA 
GGATTCTCCT CCACGCAGGA CACAGTGGTG GCTCTTCAGG CTCTGTCTTT GTACGCCACC 
AAAGTGTTCA GCTCTGACGG CTCCAGCACA GTGACTGTAC AGTCAGCAGG AGACTCTCAC 
CACTTTGATG TCAATCAGGA CAACAAGTTA CTGTACCAGG AGAAGCAGCT GGCCAATGTG 
CCGGGCAAAT ACAGCATTGA AGTCAAGGGC TCAGCCTGTG TGTCTGTGCA GATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289986.1
CDS	45..4061
Translation	MMALIHKRGLLITCFLLFVSQGTSKPNFMVTFPAIIRSGSEAKLCMSLLKPEKNLQLTITLVSSNQNRTLLQEKTEKEFHRCLDFQAPESASVQEIYVEAQGKYTHLIEKRKVKFVSHNPLTLIKTDKPIYNPGKTVQFRIFTIDFDFKPVDETYKLIAIQDNRGNRINQWINATSTHGKILQLSHSLNPEASVGLYELSVETNKGKFLEFFQVKEYVLPKFEVKIHKPKLVRGNEKEVKLGVCGRYTHGRPVLGRVKMTLCSSRIINSYFGSHYQIPFCVTGSAVLNKTGCAFEMFPLSSFIQKSVVEYITSQSLEFTASITEEGTGITEGGTEKVTLSYIIGTVKFIDVPDFYEEGSVIKAKVKVELFNGQPVSNIKLVVYVPSGIGELITDINGLANFTIDTSFGIGTTLILTASLLSTPEFVPFSFDSAHKTLQKKPKQDSFNKSQLDIKALEEPLKCGVEISVTVQYTIARETAESVTIVYLVLSRAQIVSHGYESIELKGSDRVVKGEVTFKVPFRSRMAPEIQFLAYCVLPSENVLAASKTFQTEKCFENKVSLRFSPTTAVPGEENTLQLSAQPGSLCGISAVDQSVLILESGQRLDADKVFKMLPTMTFTYDVEDQMECLKFRSKRSLRFMPAISQYGAVPSYHNMYPPLSGSTGDAVFETFKNMGLKVATNLVIRIPHCIKFRDVLFQRAYGGYGVGFGYGAVPRGIDPPAIPALAGAPGAGALIPARGGVVAEAERLIPSPLPTLSISLLPEETIRTFFPETWIWEIVEVGDSGSAEVPVTVPDTITSWATEAFCLSSTGLGLAPPAKLIVFQPFFLELSLPYSIVRGEIFELKATVFNYQSKCIMVKVSPAPSSDYTLKASSDDQYSSCLCANGRKTFKWILTPSVLGVVNVTVSAEAEASQTVCDNEIVSVPERGRIDTVTRSLRVNAEGIEKTKSHSWLLCPKGQNHLEEVELAFPQNVIEGSGRATVSVLGDLLGRALKNLDGLLRMPYGCGEQNIAVLSPNIYILQYLENTNQLTSAIRERASGFLKSGYQRQLNYLNLDGSYTTFGQGEGNTWLTAFVLRTFGKAQDYIFIDPPKIEKSKEWLVSHQRPDGSYQIKGKLFNNRMKGGVSDSVTITAYITASLLELNIPVTDPAVSKGLSHLKQYAGDIRNLYTSALLSYTFSLAKDKEVRDSLLNKLKNIAISEGPLVHWSQSASADDSDSLDVEISSYVLLAVLTADSLTTADLGFANRIVSWLVKQQNAYGGFSSTQDTVVALQALSLYATKVFSSDGSSTVTVQSAGDSHHFDVNQDNKLLYQEKQLANVPGKYSIEVKGSACVSVQI

Target ORF information:

RefSeq Version	XM_009291711.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio alpha-2-macroglobulin-like protein 1 (LOC569025), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291711.3

ORF Insert Sequence:

ATGATGGCTT TGATTCACAA AAGAGGACTA TTAATAACAT GTTTTTTGTT GTTTGTCAGT 
CAAGGAACAT CTAAACCGAA TTTTATGGTG ACATTTCCAG CCATTATAAG ATCAGGCTCA 
GAGGCTAAAT TATGTATGAG CCTTCTGAAA CCAGAAAAAA ATCTGCAGCT TACCATTACT 
CTGGTTAGCA GTAACCAGAA CCGAACACTT CTGCAGGAGA AAACAGAGAA GGAATTCCAT 
CGCTGCCTTG ACTTTCAAGC TCCTGAGTCA GCGTCTGTGC AGGAGATTTA TGTGGAAGCT 
CAAGGGAAAT ACACACATCT GATCGAAAAG AGAAAAGTCA AGTTTGTTTC ACATAATCCA 
CTGACTCTAA TAAAAACTGA TAAACCAATC TACAATCCTG GAAAAACAGT TCAATTCAGG 
ATTTTCACAA TTGACTTTGA CTTTAAACCA GTTGATGAAA CCTACAAACT GATAGCAATC 
CAGGACAATC GAGGGAACAG AATTAATCAG TGGATTAATG CCACATCCAC CCATGGCAAA 
ATTCTGCAGC TTTCCCATTC ATTGAACCCA GAGGCTTCTG TTGGTTTATA TGAGCTGTCA 
GTTGAAACCA ACAAGGGAAA ATTTCTAGAA TTTTTTCAGG TTAAAGAATA TGTTCTACCG 
AAATTCGAAG TGAAAATACA TAAGCCAAAG TTAGTAAGAG GTAATGAAAA GGAAGTAAAG 
CTGGGGGTGT GCGGAAGATA CACCCATGGA CGACCTGTTC TGGGAAGAGT AAAGATGACA 
CTCTGCTCAT CCAGAATAAT CAATTCTTAT TTCGGTTCCC ATTATCAAAT TCCATTTTGT 
GTTACTGGGT CAGCAGTACT GAACAAGACT GGGTGCGCCT TTGAGATGTT CCCCTTGTCA 
TCTTTTATTC AAAAATCAGT AGTGGAATAC ATTACATCGC AGTCTCTCGA GTTCACTGCA 
TCCATTACTG AGGAGGGAAC AGGCATCACT GAGGGGGGGA CCGAAAAAGT CACATTGTCA 
TATATTATTG GTACAGTCAA ATTTATTGAC GTGCCTGATT TTTATGAGGA AGGATCAGTC 
ATAAAGGCCA AGGTTAAAGT TGAGCTATTC AATGGACAAC CAGTTTCTAA CATCAAGTTA 
GTTGTCTACG TCCCATCTGG GATTGGAGAA CTAATCACAG ACATCAATGG GCTGGCTAAT 
TTCACCATTG ATACGTCCTT TGGGATAGGT ACTACTTTAA TTTTAACGGC AAGTTTATTA 
AGCACACCAG AGTTTGTTCC ATTCAGTTTT GATTCTGCGC ACAAAACCCT GCAAAAAAAG 
CCCAAACAAG ATTCATTTAA TAAGAGCCAA CTGGATATAA AGGCACTGGA GGAACCGCTG 
AAGTGTGGGG TGGAGATTTC AGTGACCGTG CAGTACACTA TTGCTCGAGA GACTGCTGAA 
AGTGTTACCA TAGTCTATCT GGTTTTATCA AGAGCACAAA TAGTCTCCCA CGGATATGAG 
AGCATTGAGC TGAAAGGCTC TGACAGAGTA GTGAAGGGAG AAGTGACGTT TAAAGTCCCT 
TTTCGCTCTC GGATGGCTCC AGAAATACAG TTTTTGGCAT ACTGTGTGCT GCCCAGTGAG 
AATGTACTTG CTGCGTCCAA AACATTTCAA ACTGAGAAAT GTTTTGAAAA CAAGGTCTCG 
CTGCGGTTTT CACCAACTAC TGCAGTTCCA GGTGAAGAAA ACACTCTCCA GCTCTCAGCT 
CAGCCTGGTT CACTGTGTGG TATCAGTGCT GTAGATCAGA GTGTCCTGAT CCTGGAGTCA 
GGACAACGCC TGGATGCTGA CAAAGTGTTT AAAATGTTGC CAACAATGAC TTTTACATAC 
GATGTTGAAG ATCAGATGGA ATGCTTGAAA TTTAGATCCA AAAGAAGTTT GCGGTTTATG 
CCAGCTATTT CTCAATATGG AGCAGTACCA TCCTACCATA ATATGTACCC CCCACTTTCA 
GGGAGTACAG GAGATGCTGT GTTTGAGACT TTCAAGAACA TGGGATTAAA GGTGGCAACA 
AATTTGGTTA TTAGAATCCC ACATTGTATT AAATTCAGAG ATGTATTGTT TCAGAGAGCA 
TATGGTGGTT ATGGTGTTGG TTTTGGTTAT GGAGCTGTTC CACGTGGAAT AGATCCTCCT 
GCAATTCCGG CTTTAGCAGG AGCACCAGGT GCAGGAGCCC TTATTCCAGC AAGAGGTGGA 
GTCGTAGCTG AAGCTGAAAG ACTTATACCA AGCCCACTTC CAACTTTGAG CATCTCTCTC 
TTGCCTGAGG AGACAATTCG CACATTCTTT CCAGAAACTT GGATCTGGGA AATTGTGGAG 
GTTGGAGACT CTGGATCAGC TGAGGTTCCT GTCACAGTTC CTGACACCAT CACATCTTGG 
GCGACGGAGG CCTTCTGTCT GTCCTCCACA GGTCTGGGTT TGGCTCCTCC TGCTAAGCTG 
ATAGTTTTCC AGCCCTTCTT CCTGGAGCTC TCTCTGCCTT ACTCCATTGT TCGTGGGGAG 
ATCTTTGAGC TCAAGGCCAC TGTCTTCAAC TATCAGTCCA AGTGCATCAT GGTTAAAGTG 
AGTCCAGCTC CTTCCTCAGA CTACACTCTC AAAGCCTCCT CTGATGATCA GTATTCATCC 
TGTCTCTGTG CTAATGGAAG AAAAACCTTT AAATGGATCC TTACTCCTTC TGTTCTTGGA 
GTGGTGAATG TTACAGTCAG TGCAGAGGCA GAGGCGTCCC AGACTGTGTG TGACAATGAG 
ATTGTGAGTG TGCCAGAGAG AGGACGCATT GACACCGTCA CACGGAGTCT GCGTGTCAAT 
GCTGAAGGAA TTGAAAAAAC TAAAAGCCAC AGCTGGCTGC TTTGCCCCAA AGGGCAAAAT 
CACTTGGAGG AGGTAGAGCT GGCTTTTCCA CAAAATGTGA TAGAGGGTTC AGGAAGAGCC 
ACTGTTTCAG TGCTTGGAGA CTTATTGGGC CGTGCTCTGA AGAACCTTGA TGGTTTATTA 
CGAATGCCGT ACGGCTGTGG AGAACAAAAT ATTGCTGTTC TTTCTCCCAA CATTTACATT 
CTGCAGTATC TGGAGAACAC AAATCAGCTC ACATCCGCCA TTCGAGAGAG AGCCTCTGGC 
TTCCTTAAGA GCGGTTATCA GAGGCAACTG AACTATTTGA ATTTGGATGG GTCATATACA 
ACATTTGGTC AAGGTGAAGG CAACACATGG CTGACCGCGT TTGTGCTGAG GACTTTCGGG 
AAAGCACAAG ATTACATCTT CATTGACCCC CCAAAAATCG AGAAATCAAA GGAATGGCTG 
GTGAGCCATC AACGACCAGA TGGTTCATAT CAAATCAAGG GAAAACTCTT TAATAACAGA 
ATGAAGGGTG GTGTAAGTGA CAGCGTGACC ATCACTGCCT ATATAACCGC ATCACTACTT 
GAGCTGAACA TACCAGTTAC GGATCCGGCT GTCAGTAAAG GCCTTTCACA CTTAAAGCAG 
TATGCTGGTG ATATTCGTAA CCTTTACACC TCTGCACTGC TAAGCTATAC TTTCAGTTTG 
GCAAAAGACA AAGAGGTTCG GGACAGCCTT TTGAACAAGC TTAAGAACAT TGCGATTTCA 
GAAGGTCCTC TTGTCCACTG GTCTCAGTCT GCATCTGCTG ATGACTCTGA TTCTCTGGAT 
GTGGAGATCA GCTCATATGT GCTGCTAGCT GTTCTCACTG CAGATTCACT CACTACAGCT 
GATCTGGGCT TTGCTAACAG GATTGTCAGC TGGCTTGTGA AGCAGCAGAA TGCCTATGGA 
GGATTCTCCT CCACGCAGGA CACAGTGGTG GCTCTTCAGG CTCTGTCTTT GTACGCCACC 
AAAGTGTTCA GCTCTGACGG CTCCAGCACA GTGACTGTAC AGTCAGCAGG AGACTCTCAC 
CACTTTGATG TCAATCAGGA CAACAAGTTA CTGTACCAGG AGAAGCAGCT GGCCAATGTG 
CCGGGCAAAT ACAGCATTGA AGTCAAGGGC TCAGCCTGTG TGTCTGTGCA GATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC569025 ( XM_009291711.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009289986.1 Danio rerio alpha-2-macroglobulin-like protein 1 (LOC569025), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa45636
Clone ID Related Accession (Same CDS sequence)	XM_009291711.3 , XM_009291711.2
Accession Version	XM_009291711.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	alpha-2-macroglobulin-like protein 1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291711.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTT TGATTCACAA AAGAGGACTA TTAATAACAT GTTTTTTGTT GTTTGTCAGT 
CAAGGAACAT CTAAACCGAA TTTTATGGTG ACATTTCCAG CCATTATAAG ATCAGGCTCA 
GAGGCTAAAT TATGTATGAG CCTTCTGAAA CCAGAAAAAA ATCTGCAGCT TACCATTACT 
CTGGTTAGCA GTAACCAGAA CCGAACACTT CTGCAGGAGA AAACAGAGAA GGAATTCCAT 
CGCTGCCTTG ACTTTCAAGC TCCTGAGTCA GCGTCTGTGC AGGAGATTTA TGTGGAAGCT 
CAAGGGAAAT ACACACATCT GATCGAAAAG AGAAAAGTCA AGTTTGTTTC ACATAATCCA 
CTGACTCTAA TAAAAACTGA TAAACCAATC TACAATCCTG GAAAAACAGT TCAATTCAGG 
ATTTTCACAA TTGACTTTGA CTTTAAACCA GTTGATGAAA CCTACAAACT GATAGCAATC 
CAGGACAATC GAGGGAACAG AATTAATCAG TGGATTAATG CCACATCCAC CCATGGCAAA 
ATTCTGCAGC TTTCCCATTC ATTGAACCCA GAGGCTTCTG TTGGTTTATA TGAGCTGTCA 
GTTGAAACCA ACAAGGGAAA ATTTCTAGAA TTTTTTCAGG TTAAAGAATA TGTTCTACCG 
AAATTCGAAG TGAAAATACA TAAGCCAAAG TTAGTAAGAG GTAATGAAAA GGAAGTAAAG 
CTGGGGGTGT GCGGAAGATA CACCCATGGA CGACCTGTTC TGGGAAGAGT AAAGATGACA 
CTCTGCTCAT CCAGAATAAT CAATTCTTAT TTCGGTTCCC ATTATCAAAT TCCATTTTGT 
GTTACTGGGT CAGCAGTACT GAACAAGACT GGGTGCGCCT TTGAGATGTT CCCCTTGTCA 
TCTTTTATTC AAAAATCAGT AGTGGAATAC ATTACATCGC AGTCTCTCGA GTTCACTGCA 
TCCATTACTG AGGAGGGAAC AGGCATCACT GAGGGGGGGA CCGAAAAAGT CACATTGTCA 
TATATTATTG GTACAGTCAA ATTTATTGAC GTGCCTGATT TTTATGAGGA AGGATCAGTC 
ATAAAGGCCA AGGTTAAAGT TGAGCTATTC AATGGACAAC CAGTTTCTAA CATCAAGTTA 
GTTGTCTACG TCCCATCTGG GATTGGAGAA CTAATCACAG ACATCAATGG GCTGGCTAAT 
TTCACCATTG ATACGTCCTT TGGGATAGGT ACTACTTTAA TTTTAACGGC AAGTTTATTA 
AGCACACCAG AGTTTGTTCC ATTCAGTTTT GATTCTGCGC ACAAAACCCT GCAAAAAAAG 
CCCAAACAAG ATTCATTTAA TAAGAGCCAA CTGGATATAA AGGCACTGGA GGAACCGCTG 
AAGTGTGGGG TGGAGATTTC AGTGACCGTG CAGTACACTA TTGCTCGAGA GACTGCTGAA 
AGTGTTACCA TAGTCTATCT GGTTTTATCA AGAGCACAAA TAGTCTCCCA CGGATATGAG 
AGCATTGAGC TGAAAGGCTC TGACAGAGTA GTGAAGGGAG AAGTGACGTT TAAAGTCCCT 
TTTCGCTCTC GGATGGCTCC AGAAATACAG TTTTTGGCAT ACTGTGTGCT GCCCAGTGAG 
AATGTACTTG CTGCGTCCAA AACATTTCAA ACTGAGAAAT GTTTTGAAAA CAAGGTCTCG 
CTGCGGTTTT CACCAACTAC TGCAGTTCCA GGTGAAGAAA ACACTCTCCA GCTCTCAGCT 
CAGCCTGGTT CACTGTGTGG TATCAGTGCT GTAGATCAGA GTGTCCTGAT CCTGGAGTCA 
GGACAACGCC TGGATGCTGA CAAAGTGTTT AAAATGTTGC CAACAATGAC TTTTACATAC 
GATGTTGAAG ATCAGATGGA ATGCTTGAAA TTTAGATCCA AAAGAAGTTT GCGGTTTATG 
CCAGCTATTT CTCAATATGG AGCAGTACCA TCCTACCATA ATATGTACCC CCCACTTTCA 
GGGAGTACAG GAGATGCTGT GTTTGAGACT TTCAAGAACA TGGGATTAAA GGTGGCAACA 
AATTTGGTTA TTAGAATCCC ACATTGTATT AAATTCAGAG ATGTATTGTT TCAGAGAGCA 
TATGGTGGTT ATGGTGTTGG TTTTGGTTAT GGAGCTGTTC CACGTGGAAT AGATCCTCCT 
GCAATTCCGG CTTTAGCAGG AGCACCAGGT GCAGGAGCCC TTATTCCAGC AAGAGGTGGA 
GTCGTAGCTG AAGCTGAAAG ACTTATACCA AGCCCACTTC CAACTTTGAG CATCTCTCTC 
TTGCCTGAGG AGACAATTCG CACATTCTTT CCAGAAACTT GGATCTGGGA AATTGTGGAG 
GTTGGAGACT CTGGATCAGC TGAGGTTCCT GTCACAGTTC CTGACACCAT CACATCTTGG 
GCGACGGAGG CCTTCTGTCT GTCCTCCACA GGTCTGGGTT TGGCTCCTCC TGCTAAGCTG 
ATAGTTTTCC AGCCCTTCTT CCTGGAGCTC TCTCTGCCTT ACTCCATTGT TCGTGGGGAG 
ATCTTTGAGC TCAAGGCCAC TGTCTTCAAC TATCAGTCCA AGTGCATCAT GGTTAAAGTG 
AGTCCAGCTC CTTCCTCAGA CTACACTCTC AAAGCCTCCT CTGATGATCA GTATTCATCC 
TGTCTCTGTG CTAATGGAAG AAAAACCTTT AAATGGATCC TTACTCCTTC TGTTCTTGGA 
GTGGTGAATG TTACAGTCAG TGCAGAGGCA GAGGCGTCCC AGACTGTGTG TGACAATGAG 
ATTGTGAGTG TGCCAGAGAG AGGACGCATT GACACCGTCA CACGGAGTCT GCGTGTCAAT 
GCTGAAGGAA TTGAAAAAAC TAAAAGCCAC AGCTGGCTGC TTTGCCCCAA AGGGCAAAAT 
CACTTGGAGG AGGTAGAGCT GGCTTTTCCA CAAAATGTGA TAGAGGGTTC AGGAAGAGCC 
ACTGTTTCAG TGCTTGGAGA CTTATTGGGC CGTGCTCTGA AGAACCTTGA TGGTTTATTA 
CGAATGCCGT ACGGCTGTGG AGAACAAAAT ATTGCTGTTC TTTCTCCCAA CATTTACATT 
CTGCAGTATC TGGAGAACAC AAATCAGCTC ACATCCGCCA TTCGAGAGAG AGCCTCTGGC 
TTCCTTAAGA GCGGTTATCA GAGGCAACTG AACTATTTGA ATTTGGATGG GTCATATACA 
ACATTTGGTC AAGGTGAAGG CAACACATGG CTGACCGCGT TTGTGCTGAG GACTTTCGGG 
AAAGCACAAG ATTACATCTT CATTGACCCC CCAAAAATCG AGAAATCAAA GGAATGGCTG 
GTGAGCCATC AACGACCAGA TGGTTCATAT CAAATCAAGG GAAAACTCTT TAATAACAGA 
ATGAAGGGTG GTGTAAGTGA CAGCGTGACC ATCACTGCCT ATATAACCGC ATCACTACTT 
GAGCTGAACA TACCAGTTAC GGATCCGGCT GTCAGTAAAG GCCTTTCACA CTTAAAGCAG 
TATGCTGGTG ATATTCGTAA CCTTTACACC TCTGCACTGC TAAGCTATAC TTTCAGTTTG 
GCAAAAGACA AAGAGGTTCG GGACAGCCTT TTGAACAAGC TTAAGAACAT TGCGATTTCA 
GAAGGTCCTC TTGTCCACTG GTCTCAGTCT GCATCTGCTG ATGACTCTGA TTCTCTGGAT 
GTGGAGATCA GCTCATATGT GCTGCTAGCT GTTCTCACTG CAGATTCACT CACTACAGCT 
GATCTGGGCT TTGCTAACAG GATTGTCAGC TGGCTTGTGA AGCAGCAGAA TGCCTATGGA 
GGATTCTCCT CCACGCAGGA CACAGTGGTG GCTCTTCAGG CTCTGTCTTT GTACGCCACC 
AAAGTGTTCA GCTCTGACGG CTCCAGCACA GTGACTGTAC AGTCAGCAGG AGACTCTCAC 
CACTTTGATG TCAATCAGGA CAACAAGTTA CTGTACCAGG AGAAGCAGCT GGCCAATGTG 
CCGGGCAAAT ACAGCATTGA AGTCAAGGGC TCAGCCTGTG TGTCTGTGCA GATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289986.1
CDS	32..4048
Translation	MMALIHKRGLLITCFLLFVSQGTSKPNFMVTFPAIIRSGSEAKLCMSLLKPEKNLQLTITLVSSNQNRTLLQEKTEKEFHRCLDFQAPESASVQEIYVEAQGKYTHLIEKRKVKFVSHNPLTLIKTDKPIYNPGKTVQFRIFTIDFDFKPVDETYKLIAIQDNRGNRINQWINATSTHGKILQLSHSLNPEASVGLYELSVETNKGKFLEFFQVKEYVLPKFEVKIHKPKLVRGNEKEVKLGVCGRYTHGRPVLGRVKMTLCSSRIINSYFGSHYQIPFCVTGSAVLNKTGCAFEMFPLSSFIQKSVVEYITSQSLEFTASITEEGTGITEGGTEKVTLSYIIGTVKFIDVPDFYEEGSVIKAKVKVELFNGQPVSNIKLVVYVPSGIGELITDINGLANFTIDTSFGIGTTLILTASLLSTPEFVPFSFDSAHKTLQKKPKQDSFNKSQLDIKALEEPLKCGVEISVTVQYTIARETAESVTIVYLVLSRAQIVSHGYESIELKGSDRVVKGEVTFKVPFRSRMAPEIQFLAYCVLPSENVLAASKTFQTEKCFENKVSLRFSPTTAVPGEENTLQLSAQPGSLCGISAVDQSVLILESGQRLDADKVFKMLPTMTFTYDVEDQMECLKFRSKRSLRFMPAISQYGAVPSYHNMYPPLSGSTGDAVFETFKNMGLKVATNLVIRIPHCIKFRDVLFQRAYGGYGVGFGYGAVPRGIDPPAIPALAGAPGAGALIPARGGVVAEAERLIPSPLPTLSISLLPEETIRTFFPETWIWEIVEVGDSGSAEVPVTVPDTITSWATEAFCLSSTGLGLAPPAKLIVFQPFFLELSLPYSIVRGEIFELKATVFNYQSKCIMVKVSPAPSSDYTLKASSDDQYSSCLCANGRKTFKWILTPSVLGVVNVTVSAEAEASQTVCDNEIVSVPERGRIDTVTRSLRVNAEGIEKTKSHSWLLCPKGQNHLEEVELAFPQNVIEGSGRATVSVLGDLLGRALKNLDGLLRMPYGCGEQNIAVLSPNIYILQYLENTNQLTSAIRERASGFLKSGYQRQLNYLNLDGSYTTFGQGEGNTWLTAFVLRTFGKAQDYIFIDPPKIEKSKEWLVSHQRPDGSYQIKGKLFNNRMKGGVSDSVTITAYITASLLELNIPVTDPAVSKGLSHLKQYAGDIRNLYTSALLSYTFSLAKDKEVRDSLLNKLKNIAISEGPLVHWSQSASADDSDSLDVEISSYVLLAVLTADSLTTADLGFANRIVSWLVKQQNAYGGFSSTQDTVVALQALSLYATKVFSSDGSSTVTVQSAGDSHHFDVNQDNKLLYQEKQLANVPGKYSIEVKGSACVSVQI

Target ORF information:

RefSeq Version	XM_009291711.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio alpha-2-macroglobulin-like protein 1 (LOC569025), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291711.2

ORF Insert Sequence:

ATGATGGCTT TGATTCACAA AAGAGGACTA TTAATAACAT GTTTTTTGTT GTTTGTCAGT 
CAAGGAACAT CTAAACCGAA TTTTATGGTG ACATTTCCAG CCATTATAAG ATCAGGCTCA 
GAGGCTAAAT TATGTATGAG CCTTCTGAAA CCAGAAAAAA ATCTGCAGCT TACCATTACT 
CTGGTTAGCA GTAACCAGAA CCGAACACTT CTGCAGGAGA AAACAGAGAA GGAATTCCAT 
CGCTGCCTTG ACTTTCAAGC TCCTGAGTCA GCGTCTGTGC AGGAGATTTA TGTGGAAGCT 
CAAGGGAAAT ACACACATCT GATCGAAAAG AGAAAAGTCA AGTTTGTTTC ACATAATCCA 
CTGACTCTAA TAAAAACTGA TAAACCAATC TACAATCCTG GAAAAACAGT TCAATTCAGG 
ATTTTCACAA TTGACTTTGA CTTTAAACCA GTTGATGAAA CCTACAAACT GATAGCAATC 
CAGGACAATC GAGGGAACAG AATTAATCAG TGGATTAATG CCACATCCAC CCATGGCAAA 
ATTCTGCAGC TTTCCCATTC ATTGAACCCA GAGGCTTCTG TTGGTTTATA TGAGCTGTCA 
GTTGAAACCA ACAAGGGAAA ATTTCTAGAA TTTTTTCAGG TTAAAGAATA TGTTCTACCG 
AAATTCGAAG TGAAAATACA TAAGCCAAAG TTAGTAAGAG GTAATGAAAA GGAAGTAAAG 
CTGGGGGTGT GCGGAAGATA CACCCATGGA CGACCTGTTC TGGGAAGAGT AAAGATGACA 
CTCTGCTCAT CCAGAATAAT CAATTCTTAT TTCGGTTCCC ATTATCAAAT TCCATTTTGT 
GTTACTGGGT CAGCAGTACT GAACAAGACT GGGTGCGCCT TTGAGATGTT CCCCTTGTCA 
TCTTTTATTC AAAAATCAGT AGTGGAATAC ATTACATCGC AGTCTCTCGA GTTCACTGCA 
TCCATTACTG AGGAGGGAAC AGGCATCACT GAGGGGGGGA CCGAAAAAGT CACATTGTCA 
TATATTATTG GTACAGTCAA ATTTATTGAC GTGCCTGATT TTTATGAGGA AGGATCAGTC 
ATAAAGGCCA AGGTTAAAGT TGAGCTATTC AATGGACAAC CAGTTTCTAA CATCAAGTTA 
GTTGTCTACG TCCCATCTGG GATTGGAGAA CTAATCACAG ACATCAATGG GCTGGCTAAT 
TTCACCATTG ATACGTCCTT TGGGATAGGT ACTACTTTAA TTTTAACGGC AAGTTTATTA 
AGCACACCAG AGTTTGTTCC ATTCAGTTTT GATTCTGCGC ACAAAACCCT GCAAAAAAAG 
CCCAAACAAG ATTCATTTAA TAAGAGCCAA CTGGATATAA AGGCACTGGA GGAACCGCTG 
AAGTGTGGGG TGGAGATTTC AGTGACCGTG CAGTACACTA TTGCTCGAGA GACTGCTGAA 
AGTGTTACCA TAGTCTATCT GGTTTTATCA AGAGCACAAA TAGTCTCCCA CGGATATGAG 
AGCATTGAGC TGAAAGGCTC TGACAGAGTA GTGAAGGGAG AAGTGACGTT TAAAGTCCCT 
TTTCGCTCTC GGATGGCTCC AGAAATACAG TTTTTGGCAT ACTGTGTGCT GCCCAGTGAG 
AATGTACTTG CTGCGTCCAA AACATTTCAA ACTGAGAAAT GTTTTGAAAA CAAGGTCTCG 
CTGCGGTTTT CACCAACTAC TGCAGTTCCA GGTGAAGAAA ACACTCTCCA GCTCTCAGCT 
CAGCCTGGTT CACTGTGTGG TATCAGTGCT GTAGATCAGA GTGTCCTGAT CCTGGAGTCA 
GGACAACGCC TGGATGCTGA CAAAGTGTTT AAAATGTTGC CAACAATGAC TTTTACATAC 
GATGTTGAAG ATCAGATGGA ATGCTTGAAA TTTAGATCCA AAAGAAGTTT GCGGTTTATG 
CCAGCTATTT CTCAATATGG AGCAGTACCA TCCTACCATA ATATGTACCC CCCACTTTCA 
GGGAGTACAG GAGATGCTGT GTTTGAGACT TTCAAGAACA TGGGATTAAA GGTGGCAACA 
AATTTGGTTA TTAGAATCCC ACATTGTATT AAATTCAGAG ATGTATTGTT TCAGAGAGCA 
TATGGTGGTT ATGGTGTTGG TTTTGGTTAT GGAGCTGTTC CACGTGGAAT AGATCCTCCT 
GCAATTCCGG CTTTAGCAGG AGCACCAGGT GCAGGAGCCC TTATTCCAGC AAGAGGTGGA 
GTCGTAGCTG AAGCTGAAAG ACTTATACCA AGCCCACTTC CAACTTTGAG CATCTCTCTC 
TTGCCTGAGG AGACAATTCG CACATTCTTT CCAGAAACTT GGATCTGGGA AATTGTGGAG 
GTTGGAGACT CTGGATCAGC TGAGGTTCCT GTCACAGTTC CTGACACCAT CACATCTTGG 
GCGACGGAGG CCTTCTGTCT GTCCTCCACA GGTCTGGGTT TGGCTCCTCC TGCTAAGCTG 
ATAGTTTTCC AGCCCTTCTT CCTGGAGCTC TCTCTGCCTT ACTCCATTGT TCGTGGGGAG 
ATCTTTGAGC TCAAGGCCAC TGTCTTCAAC TATCAGTCCA AGTGCATCAT GGTTAAAGTG 
AGTCCAGCTC CTTCCTCAGA CTACACTCTC AAAGCCTCCT CTGATGATCA GTATTCATCC 
TGTCTCTGTG CTAATGGAAG AAAAACCTTT AAATGGATCC TTACTCCTTC TGTTCTTGGA 
GTGGTGAATG TTACAGTCAG TGCAGAGGCA GAGGCGTCCC AGACTGTGTG TGACAATGAG 
ATTGTGAGTG TGCCAGAGAG AGGACGCATT GACACCGTCA CACGGAGTCT GCGTGTCAAT 
GCTGAAGGAA TTGAAAAAAC TAAAAGCCAC AGCTGGCTGC TTTGCCCCAA AGGGCAAAAT 
CACTTGGAGG AGGTAGAGCT GGCTTTTCCA CAAAATGTGA TAGAGGGTTC AGGAAGAGCC 
ACTGTTTCAG TGCTTGGAGA CTTATTGGGC CGTGCTCTGA AGAACCTTGA TGGTTTATTA 
CGAATGCCGT ACGGCTGTGG AGAACAAAAT ATTGCTGTTC TTTCTCCCAA CATTTACATT 
CTGCAGTATC TGGAGAACAC AAATCAGCTC ACATCCGCCA TTCGAGAGAG AGCCTCTGGC 
TTCCTTAAGA GCGGTTATCA GAGGCAACTG AACTATTTGA ATTTGGATGG GTCATATACA 
ACATTTGGTC AAGGTGAAGG CAACACATGG CTGACCGCGT TTGTGCTGAG GACTTTCGGG 
AAAGCACAAG ATTACATCTT CATTGACCCC CCAAAAATCG AGAAATCAAA GGAATGGCTG 
GTGAGCCATC AACGACCAGA TGGTTCATAT CAAATCAAGG GAAAACTCTT TAATAACAGA 
ATGAAGGGTG GTGTAAGTGA CAGCGTGACC ATCACTGCCT ATATAACCGC ATCACTACTT 
GAGCTGAACA TACCAGTTAC GGATCCGGCT GTCAGTAAAG GCCTTTCACA CTTAAAGCAG 
TATGCTGGTG ATATTCGTAA CCTTTACACC TCTGCACTGC TAAGCTATAC TTTCAGTTTG 
GCAAAAGACA AAGAGGTTCG GGACAGCCTT TTGAACAAGC TTAAGAACAT TGCGATTTCA 
GAAGGTCCTC TTGTCCACTG GTCTCAGTCT GCATCTGCTG ATGACTCTGA TTCTCTGGAT 
GTGGAGATCA GCTCATATGT GCTGCTAGCT GTTCTCACTG CAGATTCACT CACTACAGCT 
GATCTGGGCT TTGCTAACAG GATTGTCAGC TGGCTTGTGA AGCAGCAGAA TGCCTATGGA 
GGATTCTCCT CCACGCAGGA CACAGTGGTG GCTCTTCAGG CTCTGTCTTT GTACGCCACC 
AAAGTGTTCA GCTCTGACGG CTCCAGCACA GTGACTGTAC AGTCAGCAGG AGACTCTCAC 
CACTTTGATG TCAATCAGGA CAACAAGTTA CTGTACCAGG AGAAGCAGCT GGCCAATGTG 
CCGGGCAAAT ACAGCATTGA AGTCAAGGGC TCAGCCTGTG TGTCTGTGCA GATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC569025 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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