nusap1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	nusap1
Entrez Gene ID	567446
Full Name	nucleolar and spindle associated protein 1
Synonyms	YF-9,ankt,fb76a01,fi37a11,sb:cb490,wu:fb76a01,wu:fi37a11
General protein information	Preferred Names nucleolar and spindle associated protein 1 Names nucleolar and spindle-associated protein 1 nuSAP
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017358779.1	XP_017214268.1	nucleolar and spindle-associated protein 1 isoform X1
XM_017358779.2	XP_017214268.1	nucleolar and spindle-associated protein 1 isoform X1
NM_001145694.1	NP_001139166.1	nucleolar and spindle-associated protein 1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Nusap1	NP_001101232.1
Mus musculus (house mouse)	Nusap1	NP_598612.1
Gallus gallus (chicken)	NUSAP1	NP_001026364.1
Xenopus tropicalis (tropical clawed frog)	nusap1	NP_001136374.1
Canis lupus familiaris (dog)	NUSAP1	XP_005638329.1
Homo sapiens (human)	NUSAP1	NP_057443.2
Pan troglodytes (chimpanzee)	NUSAP1	XP_001149079.1
Bos taurus (cattle)	NUSAP1	NP_001040036.1
Danio rerio (zebrafish)	nusap1	NP_001139166.1

Gene Ontology
Bibliography

Related articles in PubMed

The Vascular Niche Regulates Hematopoietic Stem and Progenitor Cell Lodgment and Expansion via klf6a-ccl25b.
Xue Y, Lv J, Zhang C, Wang L, Ma D, Liu F
Developmental cell42(4)349-362.e4(2017 08)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Zebrafish midbrain slow-amplifying progenitors exhibit high levels of transcripts for nucleotide and ribosome biogenesis.
Recher G, Jouralet J, Brombin A, Heuz? A, Mugniery E, Hermel JM, Desnoulez S, Savy T, Herbomel P, Bourrat F, Peyri?ras N, Jamen F, Joly JS
Development (Cambridge, England)140(24)4860-9(2013 Dec)

Insm1a-mediated gene repression is essential for the formation and differentiation of M?ller glia-derived progenitors in the injured retina.
Ramachandran R, Zhao XF, Goldman D
Nature cell biology14(10)1013-23(2012 Oct)

Nusap1 is essential for neural crest cell migration in zebrafish.
Nie J, Wang H, He F, Huang H
Protein & cell1(3)259-66(2010 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

When the in vivo expression of nusap1 was knocked down through antisense oligonucleotide morpholino technology, the morphants of nusap1 showed impaired morphogenesis in the trunk and yolk extension, implying the involvement of Nusap1 in cell migration.
Title: Nusap1 is essential for neural crest cell migration in zebrafish.

The following nusap1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nusap1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa00571	NM_001145694.1 Latest version!	Danio rerio nucleolar and spindle associated protein 1 (nusap1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa54725	XM_017358779.2 Latest version!	Danio rerio nucleolar and spindle associated protein 1 (nusap1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
View Old Accession Versions >>
ODa54725	XM_017358779.1	Danio rerio nucleolar and spindle associated protein 1 (nusap1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nusap1 ( NM_001145694.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001139166.1 Danio rerio nucleolar and spindle associated protein 1 (nusap1), mRNA.

Price & Availability↑

CloneID	ODa00571
Clone ID Related Accession (Same CDS sequence)	NM_001145694.1
Accession Version	NM_001145694.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	nucleolar and spindle-associated protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX284673.9, BC124066.1 and AW777914.1. On May 9, 2009 this sequence version replaced XM_690742.3. ##Evidence-Data-START## Transcript exon combination :: BC124066.1, BC139863.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATTTAG ACTCGCTAAA ATATGCGGAA TTGCAGCGCC TCGCCAAAGA AGTGGGACTT 
AAAGCGAATA TGAAGGCTGA AAGGCTGTTG AAGGCTTTAA AAGAACACTT TTCACAACAG 
CAGCTTTCTG AGAACGGCAA TGACGCTGCC GCTTCGGACG CGCACGATTC TGTTCCTCCT 
GTGGACGCGC ACGCTGCAAC ATATGTGACC GAGCGCAGAG GAAAGGGCCG CACCGCCAAG 
AGGAAGCTCA GTGAACCAAC ACCGATCATT TCTCCGACTG AAGAAAAAAC ACCACAAGAT 
GAGGGTCGAA GGGGCTCCAA GAGAAGAAAG GTGTCTTCAG CCAAGGATAC ACAGACTCCA 
GTTCCAGCTG AAGCTTTCGC TAAAGAGAAT ACTATGGAGA AAGAATCTGG AGATCTAAAT 
CCAGATTCTG AAAGTGGTCC AACAGAAACG GGTGTGAAAG GGAGAAGAAC AGTTGGTAAA 
ATTCCCCGCC ATAAAGGTCT GATGAAGAGA AAACCTGCAA TGCGGCCGAC AACTCCTAAC 
TTCAAGAAAC TCCACGAGGC TCACTTCAAA AAGATGGAGT CCATCGATTC GTACGTCCAA 
AGAAAGAACA AACAAATGGA AGTGCTCAGG AATTCAGTTA AAGAGCTGAA GACCCTGTCA 
GAAAAGACAC TGAAGAAACC CGAGGCAAAG GCTCAAACTA AAGTGCCTTC TAGCCGAGCA 
TCTCTCTTCA GTCCTGCAGT GCAGAAGACA GCAGTGCCAG AAAGACGCAG ACTCACTCAG 
CAGCCGGTCA GCAAGTCCGC TTTAAAGGAC ACCGCCACCT TCAGGCCAAC TGTCCTCACC 
ACCAATAAAA TCAATGTCCG GTTTTCTCAA GCCACTCAAG ATAATGAATA CAAGCGTTCG 
CTGGTGAAGA CTCCAGCTCG TATGTCTCCT CACATGCCTC TCACCTCCAC TCCAGGGAGA 
AAATCTGATG TTCGTTCCAA AGCTGAACTA AACAGAAGTG CTAGCACCAT CACTAAAACA 
CCCGGGGCTA CGCCATTTGT TTTTAATGGC AACAACAGCA TTTCCACCAC GCCTGGCACC 
ACCAAGAAAA ACACATTTGA CCTGAAGGCC AGTCTCTCTC GTCCACTGAC CTACAAACCC 
CACAAAGGAA AACTGAAGCC TTTTGGAGAT GCACAGGAGA ACACGGCACT TAACAAATCC 
CAGACTGTCC CTTCACATCA GAAAAACTAC AAACAGCACC AGGTTCAAAC CCGAGAGGAA 
CGACGTGTCA AGCACACAGA AGATCGAAAG CAGAAGAAAG AGAAGATGTT GGGTGCCAGA 
CGAGGTTTAG TTATGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139166.1
CDS	121..1461
Translation	MDLDSLKYAELQRLAKEVGLKANMKAERLLKALKEHFSQQQLSENGNDAAASDAHDSVPPVDAHAATYVTERRGKGRTAKRKLSEPTPIISPTEEKTPQDEGRRGSKRRKVSSAKDTQTPVPAEAFAKENTMEKESGDLNPDSESGPTETGVKGRRTVGKIPRHKGLMKRKPAMRPTTPNFKKLHEAHFKKMESIDSYVQRKNKQMEVLRNSVKELKTLSEKTLKKPEAKAQTKVPSSRASLFSPAVQKTAVPERRRLTQQPVSKSALKDTATFRPTVLTTNKINVRFSQATQDNEYKRSLVKTPARMSPHMPLTSTPGRKSDVRSKAELNRSASTITKTPGATPFVFNGNNSISTTPGTTKKNTFDLKASLSRPLTYKPHKGKLKPFGDAQENTALNKSQTVPSHQKNYKQHQVQTREERRVKHTEDRKQKKEKMLGARRGLVMA

Target ORF information:

RefSeq Version	NM_001145694.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nucleolar and spindle associated protein 1 (nusap1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001145694.1

ORF Insert Sequence:

ATGGATTTAG ACTCGCTAAA ATATGCGGAA TTGCAGCGCC TCGCCAAAGA AGTGGGACTT 
AAAGCGAATA TGAAGGCTGA AAGGCTGTTG AAGGCTTTAA AAGAACACTT TTCACAACAG 
CAGCTTTCTG AGAACGGCAA TGACGCTGCC GCTTCGGACG CGCACGATTC TGTTCCTCCT 
GTGGACGCGC ACGCTGCAAC ATATGTGACC GAGCGCAGAG GAAAGGGCCG CACCGCCAAG 
AGGAAGCTCA GTGAACCAAC ACCGATCATT TCTCCGACTG AAGAAAAAAC ACCACAAGAT 
GAGGGTCGAA GGGGCTCCAA GAGAAGAAAG GTGTCTTCAG CCAAGGATAC ACAGACTCCA 
GTTCCAGCTG AAGCTTTCGC TAAAGAGAAT ACTATGGAGA AAGAATCTGG AGATCTAAAT 
CCAGATTCTG AAAGTGGTCC AACAGAAACG GGTGTGAAAG GGAGAAGAAC AGTTGGTAAA 
ATTCCCCGCC ATAAAGGTCT GATGAAGAGA AAACCTGCAA TGCGGCCGAC AACTCCTAAC 
TTCAAGAAAC TCCACGAGGC TCACTTCAAA AAGATGGAGT CCATCGATTC GTACGTCCAA 
AGAAAGAACA AACAAATGGA AGTGCTCAGG AATTCAGTTA AAGAGCTGAA GACCCTGTCA 
GAAAAGACAC TGAAGAAACC CGAGGCAAAG GCTCAAACTA AAGTGCCTTC TAGCCGAGCA 
TCTCTCTTCA GTCCTGCAGT GCAGAAGACA GCAGTGCCAG AAAGACGCAG ACTCACTCAG 
CAGCCGGTCA GCAAGTCCGC TTTAAAGGAC ACCGCCACCT TCAGGCCAAC TGTCCTCACC 
ACCAATAAAA TCAATGTCCG GTTTTCTCAA GCCACTCAAG ATAATGAATA CAAGCGTTCG 
CTGGTGAAGA CTCCAGCTCG TATGTCTCCT CACATGCCTC TCACCTCCAC TCCAGGGAGA 
AAATCTGATG TTCGTTCCAA AGCTGAACTA AACAGAAGTG CTAGCACCAT CACTAAAACA 
CCCGGGGCTA CGCCATTTGT TTTTAATGGC AACAACAGCA TTTCCACCAC GCCTGGCACC 
ACCAAGAAAA ACACATTTGA CCTGAAGGCC AGTCTCTCTC GTCCACTGAC CTACAAACCC 
CACAAAGGAA AACTGAAGCC TTTTGGAGAT GCACAGGAGA ACACGGCACT TAACAAATCC 
CAGACTGTCC CTTCACATCA GAAAAACTAC AAACAGCACC AGGTTCAAAC CCGAGAGGAA 
CGACGTGTCA AGCACACAGA AGATCGAAAG CAGAAGAAAG AGAAGATGTT GGGTGCCAGA 
CGAGGTTTAG TTATGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nusap1 ( XM_017358779.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214268.1 Danio rerio nucleolar and spindle associated protein 1 (nusap1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa54725
Clone ID Related Accession (Same CDS sequence)	XM_017358779.1 , XM_017358779.2
Accession Version	XM_017358779.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1263bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	nucleolar and spindle-associated protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTAG ACTCGCTAAA ATATGCGGAA TTGCAGCGCC TCGCCAAAGA AGTGGGACTT 
AAAGCGAATA TGAAGGCTGA AAGGCTGTTG AAGGCTTTAA AAGAACACTT TTCACAACAG 
CAGCTTTCTG AGAACGGCAA TGACGCTGCC GCTTCGGACG CGCACGATTC TGTTCCTCCT 
GTGGACGCGC ACGCTGCAAC ATATGTGACC GAGCGCAGAG GAAAGGGCCG CACCGCCAAG 
AGGAAGCTCA GTGAACCAAC ACCGATCATT TCTCCGACTG AAGAAAAAAC ACCACAAGAT 
GAGGGTCGAA GGGGCTCCAA GAGAAGAAAG GTGTCTTCAG CCAAGGATAC ACAGACTCCA 
GTTCCAGCTG AAGCTTTCGC TAAAGAGAAT ACTATGGAGA AAGAATCTGG AGATCTAAAT 
CCAGATTCTG AAAGTGGTCC AACAGAAACG GGTGTGAAAG ACTTCAAGAA ACTCCACGAG 
GCTCACTTCA AAAAGATGGA GTCCATCGAT TCGTACGTCC AAAGAAAGAA CAAACAAATG 
GAAGTGCTCA GGAATTCAGT TAAAGAGCTG AAGACCCTGT CAGAAAAGAC ACTGAAGAAA 
CCCGAGGCAA AGGCTCAAAC TAAAGTGCCT TCTAGCCGAG CATCTCTCTT CAGTCCTGCA 
GTGCAGAAGA CAGCAGTGCC AGAAAGACGC AGACTCACTC AGCAGCCGGT CAGCAAGTCC 
GCTTTAAAGG ACACCGCCAC CTTCAGGCCA ACTGTCCTCA CCACCAATAA AATCAATGTC 
CGGTTTTCTC AAGCCACTCA AGATAATGAA TACAAGCGTT CGCTGGTGAA GACTCCAGCT 
CGTATGTCTC CTCACATGCC TCTCACCTCC ACTCCAGGGA GAAAATCTGA TGTTCGTTCC 
AAAGCTGAAC TAAACAGAAG TGCTAGCACC ATCACTAAAA CACCCGGGGC TACGCCATTT 
GTTTTTAATG GCAACAACAG CATTTCCACC ACGCCTGGCA CCACCAAGAA AAACACATTT 
GACCTGAAGG CCAGTCTCTC TCGTCCACTG ACCTACAAAC CCCACAAAGG AAAACTGAAG 
CCTTTTGGAG ATGCACAGGA GAACACGGCA CTTAACAAAT CCCAGACTGT CCCTTCACAT 
CAGAAAAACT ACAAACAGCA CCAGGTTCAA ACCCGAGAGG AACGACGTGT CAAGCACACA 
GAAGATCGAA AGCAGAAGAA AGAGAAGATG TTGGGTGCCA GACGAGGTTT AGTTATGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214268.1
CDS	102..1364
Translation	MDLDSLKYAELQRLAKEVGLKANMKAERLLKALKEHFSQQQLSENGNDAAASDAHDSVPPVDAHAATYVTERRGKGRTAKRKLSEPTPIISPTEEKTPQDEGRRGSKRRKVSSAKDTQTPVPAEAFAKENTMEKESGDLNPDSESGPTETGVKDFKKLHEAHFKKMESIDSYVQRKNKQMEVLRNSVKELKTLSEKTLKKPEAKAQTKVPSSRASLFSPAVQKTAVPERRRLTQQPVSKSALKDTATFRPTVLTTNKINVRFSQATQDNEYKRSLVKTPARMSPHMPLTSTPGRKSDVRSKAELNRSASTITKTPGATPFVFNGNNSISTTPGTTKKNTFDLKASLSRPLTYKPHKGKLKPFGDAQENTALNKSQTVPSHQKNYKQHQVQTREERRVKHTEDRKQKKEKMLGARRGLVMA

Target ORF information:

RefSeq Version	XM_017358779.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio nucleolar and spindle associated protein 1 (nusap1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358779.1

ORF Insert Sequence:

ATGGATTTAG ACTCGCTAAA ATATGCGGAA TTGCAGCGCC TCGCCAAAGA AGTGGGACTT 
AAAGCGAATA TGAAGGCTGA AAGGCTGTTG AAGGCTTTAA AAGAACACTT TTCACAACAG 
CAGCTTTCTG AGAACGGCAA TGACGCTGCC GCTTCGGACG CGCACGATTC TGTTCCTCCT 
GTGGACGCGC ACGCTGCAAC ATATGTGACC GAGCGCAGAG GAAAGGGCCG CACCGCCAAG 
AGGAAGCTCA GTGAACCAAC ACCGATCATT TCTCCGACTG AAGAAAAAAC ACCACAAGAT 
GAGGGTCGAA GGGGCTCCAA GAGAAGAAAG GTGTCTTCAG CCAAGGATAC ACAGACTCCA 
GTTCCAGCTG AAGCTTTCGC TAAAGAGAAT ACTATGGAGA AAGAATCTGG AGATCTAAAT 
CCAGATTCTG AAAGTGGTCC AACAGAAACG GGTGTGAAAG ACTTCAAGAA ACTCCACGAG 
GCTCACTTCA AAAAGATGGA GTCCATCGAT TCGTACGTCC AAAGAAAGAA CAAACAAATG 
GAAGTGCTCA GGAATTCAGT TAAAGAGCTG AAGACCCTGT CAGAAAAGAC ACTGAAGAAA 
CCCGAGGCAA AGGCTCAAAC TAAAGTGCCT TCTAGCCGAG CATCTCTCTT CAGTCCTGCA 
GTGCAGAAGA CAGCAGTGCC AGAAAGACGC AGACTCACTC AGCAGCCGGT CAGCAAGTCC 
GCTTTAAAGG ACACCGCCAC CTTCAGGCCA ACTGTCCTCA CCACCAATAA AATCAATGTC 
CGGTTTTCTC AAGCCACTCA AGATAATGAA TACAAGCGTT CGCTGGTGAA GACTCCAGCT 
CGTATGTCTC CTCACATGCC TCTCACCTCC ACTCCAGGGA GAAAATCTGA TGTTCGTTCC 
AAAGCTGAAC TAAACAGAAG TGCTAGCACC ATCACTAAAA CACCCGGGGC TACGCCATTT 
GTTTTTAATG GCAACAACAG CATTTCCACC ACGCCTGGCA CCACCAAGAA AAACACATTT 
GACCTGAAGG CCAGTCTCTC TCGTCCACTG ACCTACAAAC CCCACAAAGG AAAACTGAAG 
CCTTTTGGAG ATGCACAGGA GAACACGGCA CTTAACAAAT CCCAGACTGT CCCTTCACAT 
CAGAAAAACT ACAAACAGCA CCAGGTTCAA ACCCGAGAGG AACGACGTGT CAAGCACACA 
GAAGATCGAA AGCAGAAGAA AGAGAAGATG TTGGGTGCCA GACGAGGTTT AGTTATGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nusap1 ( XM_017358779.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214268.1 Danio rerio nucleolar and spindle associated protein 1 (nusap1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa54725
Clone ID Related Accession (Same CDS sequence)	XM_017358779.1 , XM_017358779.2
Accession Version	XM_017358779.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1263bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	nucleolar and spindle-associated protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358779.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTAG ACTCGCTAAA ATATGCGGAA TTGCAGCGCC TCGCCAAAGA AGTGGGACTT 
AAAGCGAATA TGAAGGCTGA AAGGCTGTTG AAGGCTTTAA AAGAACACTT TTCACAACAG 
CAGCTTTCTG AGAACGGCAA TGACGCTGCC GCTTCGGACG CGCACGATTC TGTTCCTCCT 
GTGGACGCGC ACGCTGCAAC ATATGTGACC GAGCGCAGAG GAAAGGGCCG CACCGCCAAG 
AGGAAGCTCA GTGAACCAAC ACCGATCATT TCTCCGACTG AAGAAAAAAC ACCACAAGAT 
GAGGGTCGAA GGGGCTCCAA GAGAAGAAAG GTGTCTTCAG CCAAGGATAC ACAGACTCCA 
GTTCCAGCTG AAGCTTTCGC TAAAGAGAAT ACTATGGAGA AAGAATCTGG AGATCTAAAT 
CCAGATTCTG AAAGTGGTCC AACAGAAACG GGTGTGAAAG ACTTCAAGAA ACTCCACGAG 
GCTCACTTCA AAAAGATGGA GTCCATCGAT TCGTACGTCC AAAGAAAGAA CAAACAAATG 
GAAGTGCTCA GGAATTCAGT TAAAGAGCTG AAGACCCTGT CAGAAAAGAC ACTGAAGAAA 
CCCGAGGCAA AGGCTCAAAC TAAAGTGCCT TCTAGCCGAG CATCTCTCTT CAGTCCTGCA 
GTGCAGAAGA CAGCAGTGCC AGAAAGACGC AGACTCACTC AGCAGCCGGT CAGCAAGTCC 
GCTTTAAAGG ACACCGCCAC CTTCAGGCCA ACTGTCCTCA CCACCAATAA AATCAATGTC 
CGGTTTTCTC AAGCCACTCA AGATAATGAA TACAAGCGTT CGCTGGTGAA GACTCCAGCT 
CGTATGTCTC CTCACATGCC TCTCACCTCC ACTCCAGGGA GAAAATCTGA TGTTCGTTCC 
AAAGCTGAAC TAAACAGAAG TGCTAGCACC ATCACTAAAA CACCCGGGGC TACGCCATTT 
GTTTTTAATG GCAACAACAG CATTTCCACC ACGCCTGGCA CCACCAAGAA AAACACATTT 
GACCTGAAGG CCAGTCTCTC TCGTCCACTG ACCTACAAAC CCCACAAAGG AAAACTGAAG 
CCTTTTGGAG ATGCACAGGA GAACACGGCA CTTAACAAAT CCCAGACTGT CCCTTCACAT 
CAGAAAAACT ACAAACAGCA CCAGGTTCAA ACCCGAGAGG AACGACGTGT CAAGCACACA 
GAAGATCGAA AGCAGAAGAA AGAGAAGATG TTGGGTGCCA GACGAGGTTT AGTTATGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214268.1
CDS	99..1361
Translation	MDLDSLKYAELQRLAKEVGLKANMKAERLLKALKEHFSQQQLSENGNDAAASDAHDSVPPVDAHAATYVTERRGKGRTAKRKLSEPTPIISPTEEKTPQDEGRRGSKRRKVSSAKDTQTPVPAEAFAKENTMEKESGDLNPDSESGPTETGVKDFKKLHEAHFKKMESIDSYVQRKNKQMEVLRNSVKELKTLSEKTLKKPEAKAQTKVPSSRASLFSPAVQKTAVPERRRLTQQPVSKSALKDTATFRPTVLTTNKINVRFSQATQDNEYKRSLVKTPARMSPHMPLTSTPGRKSDVRSKAELNRSASTITKTPGATPFVFNGNNSISTTPGTTKKNTFDLKASLSRPLTYKPHKGKLKPFGDAQENTALNKSQTVPSHQKNYKQHQVQTREERRVKHTEDRKQKKEKMLGARRGLVMA

Target ORF information:

RefSeq Version	XM_017358779.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio nucleolar and spindle associated protein 1 (nusap1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358779.2

ORF Insert Sequence:

ATGGATTTAG ACTCGCTAAA ATATGCGGAA TTGCAGCGCC TCGCCAAAGA AGTGGGACTT 
AAAGCGAATA TGAAGGCTGA AAGGCTGTTG AAGGCTTTAA AAGAACACTT TTCACAACAG 
CAGCTTTCTG AGAACGGCAA TGACGCTGCC GCTTCGGACG CGCACGATTC TGTTCCTCCT 
GTGGACGCGC ACGCTGCAAC ATATGTGACC GAGCGCAGAG GAAAGGGCCG CACCGCCAAG 
AGGAAGCTCA GTGAACCAAC ACCGATCATT TCTCCGACTG AAGAAAAAAC ACCACAAGAT 
GAGGGTCGAA GGGGCTCCAA GAGAAGAAAG GTGTCTTCAG CCAAGGATAC ACAGACTCCA 
GTTCCAGCTG AAGCTTTCGC TAAAGAGAAT ACTATGGAGA AAGAATCTGG AGATCTAAAT 
CCAGATTCTG AAAGTGGTCC AACAGAAACG GGTGTGAAAG ACTTCAAGAA ACTCCACGAG 
GCTCACTTCA AAAAGATGGA GTCCATCGAT TCGTACGTCC AAAGAAAGAA CAAACAAATG 
GAAGTGCTCA GGAATTCAGT TAAAGAGCTG AAGACCCTGT CAGAAAAGAC ACTGAAGAAA 
CCCGAGGCAA AGGCTCAAAC TAAAGTGCCT TCTAGCCGAG CATCTCTCTT CAGTCCTGCA 
GTGCAGAAGA CAGCAGTGCC AGAAAGACGC AGACTCACTC AGCAGCCGGT CAGCAAGTCC 
GCTTTAAAGG ACACCGCCAC CTTCAGGCCA ACTGTCCTCA CCACCAATAA AATCAATGTC 
CGGTTTTCTC AAGCCACTCA AGATAATGAA TACAAGCGTT CGCTGGTGAA GACTCCAGCT 
CGTATGTCTC CTCACATGCC TCTCACCTCC ACTCCAGGGA GAAAATCTGA TGTTCGTTCC 
AAAGCTGAAC TAAACAGAAG TGCTAGCACC ATCACTAAAA CACCCGGGGC TACGCCATTT 
GTTTTTAATG GCAACAACAG CATTTCCACC ACGCCTGGCA CCACCAAGAA AAACACATTT 
GACCTGAAGG CCAGTCTCTC TCGTCCACTG ACCTACAAAC CCCACAAAGG AAAACTGAAG 
CCTTTTGGAG ATGCACAGGA GAACACGGCA CTTAACAAAT CCCAGACTGT CCCTTCACAT 
CAGAAAAACT ACAAACAGCA CCAGGTTCAA ACCCGAGAGG AACGACGTGT CAAGCACACA 
GAAGATCGAA AGCAGAAGAA AGAGAAGATG TTGGGTGCCA GACGAGGTTT AGTTATGGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Vascular Niche Regulates Hematopoietic Stem and Progenitor Cell Lodgment and Expansion via klf6a-ccl25b.
Developmental cell42(4)349-362.e4(2017 08)
Xue Y,Lv J,Zhang C,Wang L,Ma D,Liu F

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Zebrafish midbrain slow-amplifying progenitors exhibit high levels of transcripts for nucleotide and ribosome biogenesis.
Development (Cambridge, England)140(24)4860-9(2013 Dec)
Recher G,Jouralet J,Brombin A,Heuz? A,Mugniery E,Hermel JM,Desnoulez S,Savy T,Herbomel P,Bourrat F,Peyri?ras N,Jamen F,Joly JS

Insm1a-mediated gene repression is essential for the formation and differentiation of M?ller glia-derived progenitors in the injured retina.
Nature cell biology14(10)1013-23(2012 Oct)
Ramachandran R,Zhao XF,Goldman D

Nusap1 is essential for neural crest cell migration in zebrafish.
Protein & cell1(3)259-66(2010 Mar)
Nie J,Wang H,He F,Huang H