ORF » Species Summary » Xenopus tropicalis » nusap1 cDNA ORF clone

nusap1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following nusap1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nusap1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa00867 NM_001142902.1
Latest version!		 Xenopus tropicalis nucleolar and spindle associated protein 1 (nusap1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OXa28212 XM_012968505.2
Latest version!		 Xenopus tropicalis nucleolar and spindle associated protein 1 (nusap1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OXa63713 XM_012968505.3
Latest version!		 Xenopus tropicalis nucleolar and spindle associated protein 1 (nusap1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    nusap1 ( NM_001142902.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001136374.1 Xenopus tropicalis nucleolar and spindle associated protein 1 (nusap1), mRNA.
    Price & Availability↑
    CloneID OXa00867
    Clone ID Related Accession (Same CDS sequence) NM_001142902.1
    Accession Version NM_001142902.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1497bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-22
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nucleolar and spindle-associated protein 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC166075.1. ##Evidence-Data-START## Transcript exon combination :: BC166075.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGAAGCGC CGACTTTATC CCAACTGGAG GGTCTGAGGT ACTCGGAACT GCAAAAGCTG 
    GCAAAGACAG CGGGACTCAA AGCTAACCTC AAGGCTGATA AACTGCTCAA GGTTTTAAAA 
    GCACATTTCT ACCCAGAGAG CAAAAATGAG ACTCTGGATT CAGATGGAAG CTCTTCACTA 
    ACTGATACTG ATGAACTGAA CAGCAGCCAA GAGAAGGAAG AGCCAGTCTC TGTATCCTTT 
    GTTACTCACA GACGAGGCAG GGGCCGGAAA CCAATACGAA ACCAGGACAC CCCAAAAGAT 
    GAATTCCTCA CTGTCAGTGC AGGGGTTGGC ACAGAGAATA TGGCTTCATC CATAGACCGC 
    ACCCAGAACT GTACGGAGTC AAAGAAAGAA GAGACAACCT CCCCCATTAT AGACAATAAA 
    CATAGAAAAA GAAGCCGATC TGAAGACATA AGCAAGCAGA ACAACTTGGA GTCTACTGGG 
    AAGACTGAGA AGCGGCAAAA GAAAGCCAGC AATGTTACTA GTGTAGCTTC AACTGGAAAG 
    ATTCCAAGAC TTGCTGGTCG TCTGTCTAAG CCTGGATCAA AACCTTCCAC TCCAAATTTT 
    AAAAAGTTAC ACGAGGCTCA CTTCAAGAAA ATGGAATCTA TAGACAAGTA CATGGAGAGG 
    AAGCAGAAGC GCCTTGATGC AGTTAGCAGC TCTATCCAGG AAGTAAAGAT GTTAACAAAA 
    AAATCCAACC TTCTCAAGTC AGTGGAGAAA ACGCCTGTCA GCGATATAAA GAAGCCAGTA 
    AAGAGCAGGC TTTCATTGCT GAGCCCCTTA CCTCGGACCA CTGGTGCATC TCCTTCTCGC 
    ACACCCTTGA GCCAGAGACG TTCGTGCCGC TCTAGCACTG CCAGTAAGAG CATCCTGGTT 
    GATAGATCTG GGTTCAAGCC TTCGGTTTTT TCGTCATCCA AAATGAACGT TAGGTTTTCA 
    GAAGCTACAA AAGATAATGA ACATAAGCGC TCTCTTATCA AGACGCCTGC CAGGAAGTCC 
    TCTAGCTTTC TGGCTGTCAC TCCTGAGAGT GAACCAAGAC GAATTTTGCC CTCCGTAAGA 
    AAGACTGAAT TGCTGCCTGC TCCAGAAAAA GCTGATAAGC CAGATGTTAA CATCACTCTT 
    AACACCACAA CCCAACCATC TCCAGCTACC GGAATGAGCG CCTGTAAAGC TATCACACCT 
    TTCAAGTTTA CGGCTCAAAA TACAGAGACT CCAAACACAA AGAAAAAGGG TAAATTTGAT 
    CTGCAGGCAA GTCTGTCCCG GCAGCTTGGA TACCAGCCCC ACAAAGGAAA ACTTAAACCT 
    TGGGGAGAGT CTAAAGAAAA TAAACCTGAT CCCAACAGCA ATGTATCTGT TTTGAAGAAT 
    AACTACAAGC AACCCCATCT CCAGACCAGA GAGGACAGAC GAAACCAGCA TGTACAGAAT 
    AGAAAGAACA AGAGGGACCA GGCACTGGGA ACAAGGAGAG GAGTCCCTGT AAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001136374.1
    CDS47..1543
    Translation

    Target ORF information:

    RefSeq Version NM_001142902.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis nucleolar and spindle associated protein 1 (nusap1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001142902.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGAAGCGC CGACTTTATC CCAACTGGAG GGTCTGAGGT ACTCGGAACT GCAAAAGCTG 
    GCAAAGACAG CGGGACTCAA AGCTAACCTC AAGGCTGATA AACTGCTCAA GGTTTTAAAA 
    GCACATTTCT ACCCAGAGAG CAAAAATGAG ACTCTGGATT CAGATGGAAG CTCTTCACTA 
    ACTGATACTG ATGAACTGAA CAGCAGCCAA GAGAAGGAAG AGCCAGTCTC TGTATCCTTT 
    GTTACTCACA GACGAGGCAG GGGCCGGAAA CCAATACGAA ACCAGGACAC CCCAAAAGAT 
    GAATTCCTCA CTGTCAGTGC AGGGGTTGGC ACAGAGAATA TGGCTTCATC CATAGACCGC 
    ACCCAGAACT GTACGGAGTC AAAGAAAGAA GAGACAACCT CCCCCATTAT AGACAATAAA 
    CATAGAAAAA GAAGCCGATC TGAAGACATA AGCAAGCAGA ACAACTTGGA GTCTACTGGG 
    AAGACTGAGA AGCGGCAAAA GAAAGCCAGC AATGTTACTA GTGTAGCTTC AACTGGAAAG 
    ATTCCAAGAC TTGCTGGTCG TCTGTCTAAG CCTGGATCAA AACCTTCCAC TCCAAATTTT 
    AAAAAGTTAC ACGAGGCTCA CTTCAAGAAA ATGGAATCTA TAGACAAGTA CATGGAGAGG 
    AAGCAGAAGC GCCTTGATGC AGTTAGCAGC TCTATCCAGG AAGTAAAGAT GTTAACAAAA 
    AAATCCAACC TTCTCAAGTC AGTGGAGAAA ACGCCTGTCA GCGATATAAA GAAGCCAGTA 
    AAGAGCAGGC TTTCATTGCT GAGCCCCTTA CCTCGGACCA CTGGTGCATC TCCTTCTCGC 
    ACACCCTTGA GCCAGAGACG TTCGTGCCGC TCTAGCACTG CCAGTAAGAG CATCCTGGTT 
    GATAGATCTG GGTTCAAGCC TTCGGTTTTT TCGTCATCCA AAATGAACGT TAGGTTTTCA 
    GAAGCTACAA AAGATAATGA ACATAAGCGC TCTCTTATCA AGACGCCTGC CAGGAAGTCC 
    TCTAGCTTTC TGGCTGTCAC TCCTGAGAGT GAACCAAGAC GAATTTTGCC CTCCGTAAGA 
    AAGACTGAAT TGCTGCCTGC TCCAGAAAAA GCTGATAAGC CAGATGTTAA CATCACTCTT 
    AACACCACAA CCCAACCATC TCCAGCTACC GGAATGAGCG CCTGTAAAGC TATCACACCT 
    TTCAAGTTTA CGGCTCAAAA TACAGAGACT CCAAACACAA AGAAAAAGGG TAAATTTGAT 
    CTGCAGGCAA GTCTGTCCCG GCAGCTTGGA TACCAGCCCC ACAAAGGAAA ACTTAAACCT 
    TGGGGAGAGT CTAAAGAAAA TAAACCTGAT CCCAACAGCA ATGTATCTGT TTTGAAGAAT 
    AACTACAAGC AACCCCATCT CCAGACCAGA GAGGACAGAC GAAACCAGCA TGTACAGAAT 
    AGAAAGAACA AGAGGGACCA GGCACTGGGA ACAAGGAGAG GAGTCCCTGT AAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    nusap1 ( XM_012968505.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823959.1 Xenopus tropicalis nucleolar and spindle associated protein 1 (nusap1), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OXa28212
    Clone ID Related Accession (Same CDS sequence) XM_012968505.2
    Accession Version XM_012968505.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1710bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nucleolar and spindle-associated protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012968505.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGAAACTA GACGTACAAG TTGTGTGGGA GGGTATGTTT ACTCGGCTGA GCGTTCATTG 
    GCTAGAAATG ACACGCCACT GGCCAATGAT GGAGCCGAGA AGCTGAGTTT TGAAAGCAAG 
    CCGTTAGTTC TTCCTACGCG CCTAAACGTC AGTTTTGTTG TATGCAGTTG GAGCCCGTGT 
    ATACACAGGA ATCGCAACAT TGTGACAATG GAAGCGCCGA CTTTATCCCA ACTGGAGGGT 
    CTGAGGTACT CGGAACTGCA AAAGCTGGCA AAGACAGCGG GACTCAAAGC TAACCTCAAG 
    GCTGATAAAC TGCTCAAGGT TTTAAAAGCA CATTTCTACC CAGAGAGCAA AAATGAGACT 
    CTGGATTCAG ATGGAAGCTC TTCACTAACT GATACTGATG AACTGAACAG CAGCCAAGAG 
    AAGGAAGAGC CAGTCTCTGT ATCCTTTGTT ACTCACAGAC GAGGCAGGGG CCGGAAACCA 
    ATACGAAACC AGGACACCCC AAAAGATGAA TTCCTCACTG TCAGTGCAGG GGTTGGCACA 
    GAGAATATGG CTTCATCCAT AGACCGCACC CAGCAACAGA ACTGTACGGA GTCAAAGAAA 
    GAAGAGACAA CCTCCCCCAT TATAGACAAT AAACATAGAA AAAGAAGCCG ATCTGAAGAC 
    ACAAGCAAGC AGAACAACTT GGAGTCTACT GGGAAGACTG AGAAGCGGCA AAAGAAAGCC 
    AGCAATGTTA CTAGTGTAGC TTCAACTGGA AAGATTCCAA GACTTGCTGG TCGTCTGTCT 
    AAGCCTGGAT CAAAACCTTC CACTCCAAAT TTTAAAAAGT TACACGAGGC TCACTTCAAG 
    AAAATGGAAT CTATAGACAA GTACATGGAG AGGAAGCAGA AGCGCCTTGA TGCAGTTAGC 
    AGCTCTATCC AGGAAGTAAA GATGTTAACA AAAAAATCCA ACCTTCTCAA GTCAGTGGAG 
    AAAACGCCTG TCAGCGATAT AAAGAAGCCA GTAAAGAGCA GGCTTTCATT GCTGAGCCCC 
    TTACCTCGGA CCACTGGTGC ATCTCCTTCT CGCACACCCA TGAGCCAGAG ACGTTCGTGC 
    CGCTCTAGCA CTGCCAGTAA GAGCATCCTG GTTGATAGAT CTGGGTTCAA GCCTTCGGTT 
    TTTTCGTCAT CCAAAATGAA CGTTAGGTTT TCAGAAGCTA CAAAAGATAA TGAACATAAG 
    CGCTCTCTTA TCAAGACGCC TGCCAGGAAG TCCTCTAGCT TTCTGGCTGT CACTCCTGAG 
    AGTGAACCAA GACGAATTTT GCCCTCCGTA AGAAAGACTG AATTGCTGCC TGCTCCAGAA 
    AAAGCTGATA AGCCAGATGT TAACATCACT CTTAACACCA CAACCCAACC ATCTCCAGCT 
    ACCGGAATGA GCGCCTGTAA AGCTATCACA CCTTTCAAGT TTACGGCTCA AAATACAGAG 
    ACTCCAAACA CAAAGAAAAA GGGTAAATTT GATCTGCAGG CAAGTCTGTC CCGGCAGCTT 
    GGATACCAGC CCCACAAAGG AAAACTTAAA CCTTGGGGAG AGTCTAAAGA AAATAAACCT 
    GATCCCAACA GCAATGTATC TGTTTTGAAG AATAACTACA AGCAACCCCA TCTCCAGACC 
    AGAGAGGACA GACGAAACCA GCATGTACAG AATAGAAAGA ACAAGAGGGA CCAGGCACTG 
    GGAACAAGGA GAGGAGTCCC TGTAAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012823959.1
    CDS151..1860
    Translation

    Target ORF information:

    RefSeq Version XM_012968505.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis nucleolar and spindle associated protein 1 (nusap1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012968505.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGAAACTA GACGTACAAG TTGTGTGGGA GGGTATGTTT ACTCGGCTGA GCGTTCATTG 
    GCTAGAAATG ACACGCCACT GGCCAATGAT GGAGCCGAGA AGCTGAGTTT TGAAAGCAAG 
    CCGTTAGTTC TTCCTACGCG CCTAAACGTC AGTTTTGTTG TATGCAGTTG GAGCCCGTGT 
    ATACACAGGA ATCGCAACAT TGTGACAATG GAAGCGCCGA CTTTATCCCA ACTGGAGGGT 
    CTGAGGTACT CGGAACTGCA AAAGCTGGCA AAGACAGCGG GACTCAAAGC TAACCTCAAG 
    GCTGATAAAC TGCTCAAGGT TTTAAAAGCA CATTTCTACC CAGAGAGCAA AAATGAGACT 
    CTGGATTCAG ATGGAAGCTC TTCACTAACT GATACTGATG AACTGAACAG CAGCCAAGAG 
    AAGGAAGAGC CAGTCTCTGT ATCCTTTGTT ACTCACAGAC GAGGCAGGGG CCGGAAACCA 
    ATACGAAACC AGGACACCCC AAAAGATGAA TTCCTCACTG TCAGTGCAGG GGTTGGCACA 
    GAGAATATGG CTTCATCCAT AGACCGCACC CAGCAACAGA ACTGTACGGA GTCAAAGAAA 
    GAAGAGACAA CCTCCCCCAT TATAGACAAT AAACATAGAA AAAGAAGCCG ATCTGAAGAC 
    ACAAGCAAGC AGAACAACTT GGAGTCTACT GGGAAGACTG AGAAGCGGCA AAAGAAAGCC 
    AGCAATGTTA CTAGTGTAGC TTCAACTGGA AAGATTCCAA GACTTGCTGG TCGTCTGTCT 
    AAGCCTGGAT CAAAACCTTC CACTCCAAAT TTTAAAAAGT TACACGAGGC TCACTTCAAG 
    AAAATGGAAT CTATAGACAA GTACATGGAG AGGAAGCAGA AGCGCCTTGA TGCAGTTAGC 
    AGCTCTATCC AGGAAGTAAA GATGTTAACA AAAAAATCCA ACCTTCTCAA GTCAGTGGAG 
    AAAACGCCTG TCAGCGATAT AAAGAAGCCA GTAAAGAGCA GGCTTTCATT GCTGAGCCCC 
    TTACCTCGGA CCACTGGTGC ATCTCCTTCT CGCACACCCA TGAGCCAGAG ACGTTCGTGC 
    CGCTCTAGCA CTGCCAGTAA GAGCATCCTG GTTGATAGAT CTGGGTTCAA GCCTTCGGTT 
    TTTTCGTCAT CCAAAATGAA CGTTAGGTTT TCAGAAGCTA CAAAAGATAA TGAACATAAG 
    CGCTCTCTTA TCAAGACGCC TGCCAGGAAG TCCTCTAGCT TTCTGGCTGT CACTCCTGAG 
    AGTGAACCAA GACGAATTTT GCCCTCCGTA AGAAAGACTG AATTGCTGCC TGCTCCAGAA 
    AAAGCTGATA AGCCAGATGT TAACATCACT CTTAACACCA CAACCCAACC ATCTCCAGCT 
    ACCGGAATGA GCGCCTGTAA AGCTATCACA CCTTTCAAGT TTACGGCTCA AAATACAGAG 
    ACTCCAAACA CAAAGAAAAA GGGTAAATTT GATCTGCAGG CAAGTCTGTC CCGGCAGCTT 
    GGATACCAGC CCCACAAAGG AAAACTTAAA CCTTGGGGAG AGTCTAAAGA AAATAAACCT 
    GATCCCAACA GCAATGTATC TGTTTTGAAG AATAACTACA AGCAACCCCA TCTCCAGACC 
    AGAGAGGACA GACGAAACCA GCATGTACAG AATAGAAAGA ACAAGAGGGA CCAGGCACTG 
    GGAACAAGGA GAGGAGTCCC TGTAAAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    nusap1 ( XM_012968505.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012823959.2 Xenopus tropicalis nucleolar and spindle associated protein 1 (nusap1), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OXa63713
    Clone ID Related Accession (Same CDS sequence) XM_012968505.3
    Accession Version XM_012968505.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1503bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product nucleolar and spindle-associated protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012968505.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGAAGCGC CGACTTTATC CCAACTGGAG GGTCTGAGGT ACTCGGAACT GCAAAAGCTG 
    GCAAAGACAG CGGGACTCAA AGCTAACCTC AAGGCTGATA AACTGCTCAA GGTTTTAAAA 
    GCACATTTCT ACCCAGAGAG CAAAAATGAG ACTCTGGATT CAGATGGAAG CTCTTCACTA 
    ACTGATACTG ATGAACTGAA CAGCAGCCAA GAGAAGGAAG AGCCAGTCTC TGTATCCTTT 
    GTTACTCACA GACGAGGCAG GGGCCGGAAA CCAATACGAA ACCAGGACAT CCCAAAAGAT 
    GAATTCCTCA CTGTCAGTGC TGGGGTTGGC ACAGAGAATA TGGCTTCATC CATAGACCGC 
    ACCCAGCAAC AGAACTGTAC GGAGTCAAAG AAAGAAGAGA CAACCTCCCC CATTATAGAC 
    AATAAACATA GAAAAAGAAG CCGATCTGAA GACATAAGCA AGCAGAACAA CTTGGAGTCT 
    ACTGGGAAGA CTGAGAAGCG GCAAAAGAAA GCCAGCAATG TTACTAGTGT AGCTTCAACT 
    GGAAAGATTC CAAGACTTGC TGGTCGTCTG TCTAAGCCTG GATCAAAACC TTCCACTCCA 
    AATTTTAAAA AGTTACACGA GGCTCACTTC AAGAAAATGG AATCTATAGA CAAGTACATG 
    GAGAGGAAGC AGAAGCGCCT TGATGCAGTT AGCAGCTCTA TCCAGGAAGT AAAGATGTTA 
    ACAAAAAAAT CCAACCTTCT CAAGTCAGTG GAGAAAACAC CTGTCAGCGA TATAAAGAAG 
    CCAGTAAAGA GCAGGCTTTC ATTGCTGAGC CCCTTACCTC GGACCACTGG TGCATCTCCT 
    TCTCGCACAC CCTTGAGCCA GAGACGTTCA TGCCGCTCTA GCACTGCCAG TAAGAGCATC 
    CTGGTTGATA GATCTGGGTT CAAGCCTTCG GTTTTTTCGT CATCCAAAAT GAACGTTAGG 
    TTTTCAGAAG CTACAAAAGA TAATGAACAT AAGCGCTCTC TTATCAAGAC GCCTGCCAGG 
    AAGTCCTCTA GCTTTCTGGC TGTCACTCCT GAGAGTGAAC CAAGACGAAT TTTGCCCTCC 
    GTAAGAAAGA CTGAATTGCT GCCTGCTCCA GAAAAAGCTG ATAAGCCAGA TGTTAACATC 
    ACTCTTAACA CCACAACCCA ACCATCTCCA GCTACCGGAA TGAGCGCCTG TAAAGCTATC 
    ACACCTTTCA AGTTTACGGC TCAAAATACA GAGACTCCAA ACACAAAGAA AAAGGGTAAA 
    TTTGATCTGC AGGCAAGTCT GTCCCGGCAG CTTGGATACC AGCCCCACAA AGGAAAACTT 
    AAACCTTGGG GAGAGTCTAA AGAAAATAAA CCTGATCCCA ACAGCAATGT ATCTGTTTTG 
    AAGAATAACT ACAAGCAACC CCATCTCCAG ACCAGAGAGG ACAGACGAAA CCAGCATGTA 
    CAGAATAGAA AGAACAAGAG GGACCAGGCA CTGGGAACAA GGAGAGGAGT CCCTGTAAAG 
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012823959.2
    CDS417..1919
    Translation

    Target ORF information:

    RefSeq Version XM_012968505.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis nucleolar and spindle associated protein 1 (nusap1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_012968505.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGAAGCGC CGACTTTATC CCAACTGGAG GGTCTGAGGT ACTCGGAACT GCAAAAGCTG 
    GCAAAGACAG CGGGACTCAA AGCTAACCTC AAGGCTGATA AACTGCTCAA GGTTTTAAAA 
    GCACATTTCT ACCCAGAGAG CAAAAATGAG ACTCTGGATT CAGATGGAAG CTCTTCACTA 
    ACTGATACTG ATGAACTGAA CAGCAGCCAA GAGAAGGAAG AGCCAGTCTC TGTATCCTTT 
    GTTACTCACA GACGAGGCAG GGGCCGGAAA CCAATACGAA ACCAGGACAT CCCAAAAGAT 
    GAATTCCTCA CTGTCAGTGC TGGGGTTGGC ACAGAGAATA TGGCTTCATC CATAGACCGC 
    ACCCAGCAAC AGAACTGTAC GGAGTCAAAG AAAGAAGAGA CAACCTCCCC CATTATAGAC 
    AATAAACATA GAAAAAGAAG CCGATCTGAA GACATAAGCA AGCAGAACAA CTTGGAGTCT 
    ACTGGGAAGA CTGAGAAGCG GCAAAAGAAA GCCAGCAATG TTACTAGTGT AGCTTCAACT 
    GGAAAGATTC CAAGACTTGC TGGTCGTCTG TCTAAGCCTG GATCAAAACC TTCCACTCCA 
    AATTTTAAAA AGTTACACGA GGCTCACTTC AAGAAAATGG AATCTATAGA CAAGTACATG 
    GAGAGGAAGC AGAAGCGCCT TGATGCAGTT AGCAGCTCTA TCCAGGAAGT AAAGATGTTA 
    ACAAAAAAAT CCAACCTTCT CAAGTCAGTG GAGAAAACAC CTGTCAGCGA TATAAAGAAG 
    CCAGTAAAGA GCAGGCTTTC ATTGCTGAGC CCCTTACCTC GGACCACTGG TGCATCTCCT 
    TCTCGCACAC CCTTGAGCCA GAGACGTTCA TGCCGCTCTA GCACTGCCAG TAAGAGCATC 
    CTGGTTGATA GATCTGGGTT CAAGCCTTCG GTTTTTTCGT CATCCAAAAT GAACGTTAGG 
    TTTTCAGAAG CTACAAAAGA TAATGAACAT AAGCGCTCTC TTATCAAGAC GCCTGCCAGG 
    AAGTCCTCTA GCTTTCTGGC TGTCACTCCT GAGAGTGAAC CAAGACGAAT TTTGCCCTCC 
    GTAAGAAAGA CTGAATTGCT GCCTGCTCCA GAAAAAGCTG ATAAGCCAGA TGTTAACATC 
    ACTCTTAACA CCACAACCCA ACCATCTCCA GCTACCGGAA TGAGCGCCTG TAAAGCTATC 
    ACACCTTTCA AGTTTACGGC TCAAAATACA GAGACTCCAA ACACAAAGAA AAAGGGTAAA 
    TTTGATCTGC AGGCAAGTCT GTCCCGGCAG CTTGGATACC AGCCCCACAA AGGAAAACTT 
    AAACCTTGGG GAGAGTCTAA AGAAAATAAA CCTGATCCCA ACAGCAATGT ATCTGTTTTG 
    AAGAATAACT ACAAGCAACC CCATCTCCAG ACCAGAGAGG ACAGACGAAA CCAGCATGTA 
    CAGAATAGAA AGAACAAGAG GGACCAGGCA CTGGGAACAA GGAGAGGAGT CCCTGTAAAG 
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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