si:dkey-172j4.3 gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_005172317.4	XP_005172374.1	diacylglycerol kinase delta isoform X2
XM_005172316.3	XP_005172373.1	diacylglycerol kinase kappa isoform X2
XM_005172318.3	XP_005172375.1	diacylglycerol kinase kappa isoform X3
XM_005172318.4	XP_005172375.1	diacylglycerol kinase delta isoform X3
XM_009303126.3	XP_009301401.1	diacylglycerol kinase delta isoform X1
XM_021477787.1	XP_021333462.1	diacylglycerol kinase delta isoform X2
XM_005172317.3	XP_005172374.1	diacylglycerol kinase kappa isoform X2
XM_009303126.2	XP_009301401.1	diacylglycerol kinase kappa isoform X1
XM_005172316.4	XP_005172373.1	diacylglycerol kinase delta isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa12940	XM_005172317.4 Latest version!	Danio rerio si:dkey-172j4.3 (si:dkey-172j4.3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa12940	XM_021477787.1 Latest version!	Danio rerio si:dkey-172j4.3 (si:dkey-172j4.3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa12940	XM_005172316.4 Latest version!	Danio rerio si:dkey-172j4.3 (si:dkey-172j4.3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa12941	XM_005172318.4 Latest version!	Danio rerio si:dkey-172j4.3 (si:dkey-172j4.3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa44518	XM_009303126.3 Latest version!	Danio rerio si:dkey-172j4.3 (si:dkey-172j4.3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa12941	XM_005172318.3	Danio rerio si:dkey-172j4.3 (si:dkey-172j4.3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa12940	XM_005172317.3	Danio rerio si:dkey-172j4.3 (si:dkey-172j4.3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa44518	XM_009303126.2	Danio rerio si:dkey-172j4.3 (si:dkey-172j4.3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa12940	XM_005172316.3	Danio rerio si:dkey-172j4.3 (si:dkey-172j4.3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

si:dkey-172j4.3 ( XM_005172317.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172374.1 Danio rerio si:dkey-172j4.3 (si:dkey-172j4.3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa12940
Clone ID Related Accession (Same CDS sequence)	XM_005172317.4 , XM_005172316.3 , XM_021477787.1 , XM_005172317.3 , XM_005172316.4
Accession Version	XM_005172317.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	diacylglycerol kinase delta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005172317.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAA AGTTCAATCC AAACGTCCTT TATGTCCGGG AGCCCCAGGA GTCCCTGACC 
TCTCCAGACC CTCAAGTTGA GGCGGGTGAC GAATCATCAG ACAGTGACGG GGAGCCTGAA 
GAGACCTCAC ACAAACTCAT CCGAAAAGTG TCCACCTCTG GACAGATTCG AGCCAAGAAG 
AGTGTGAAGG AGGGTCTTCT GCTCAAACAG ACCAGCTCTT TTCAGAGGTG GAAGAGGAGG 
TATTTCAAAC TGCGTGGAAG GACACTTTAT TATGCCAAAG ATTCAAAGTC TCTCATATTT 
GATGAGGTGG ACCTGTCAGA TGCCAGTGTG GCCGAGACCA GCACCAAGAA CATCAACAAC 
AGTTTTACAG TCATCACTCC ATTCCGTAAG CTGATGTTGT GTGCGGAGAG CAGGAAGGAG 
ATGGAAGACT GGATCAGTGC TTTGAAGTCT GTACAGAAAT GGGAAACATA CGAGGCCAGT 
CAGTTCAACA TGGAGCACTT CTCCGGGATG CATAACTGGT ACGCCTGCTC TCATGCCCGG 
CCCACTTTCT GCAACGTGTG TCGAGAGGCT CTGCCGGGTG TTACCTCTCA TGGTCTCTCC 
TGTGAAGTGT GTAAGTTCAA AGCCCACAAG CGCTGTGCCG TACGATCCAC CAATACCTGT 
AAATGGACAA CTCTGGCATC TATAGGAAAT GACATCATCG AGGATGAGGA TGGAGTGTCA 
ATGCCTCACC AGTGGTTGGA GGGAAACCTG CCGGTCAGCG CTAAGTGTGT GGTGTGCGAT 
CGAAACTGCG GCAGCGTCCG GCGACTACAG GACTGGCGGT GTCTCTGGTG CAAAGCCATC 
GTGCACAACA GCTGTAAAGA GCAGTTGGGG AAGGTTTGTC CTCTGGGTCA GTGTAAGGTC 
TCCATCATTC CACCCACTGC ACTTAACAGC ATCGACTCTG ACGGCTTCTG GAAGGCCACC 
AGTCCATCCT GCTCCAGTCC TCTTCTGGTG CTGGTGAACA GTAAGAGTGG AGACAACCAG 
GGTGTCAAGT TCCTGCGCAG GTTTAAGCAG CTCCTGAATC CAGCGCAGGT CTTTGACCTC 
ATGAATGGCG GCCCACAGCT GGGGCTTCGT CTCTTCCAGA AGTTTGAGAC ATTTCGTACC 
CTGGTGTGCG GTGGAGATGG GAGCGTGGGC TGGGTTCTTT CTGAACTGGA CAAACTCAGC 
CTGCACAAAC AGTGTCAGCT GGGTGTGCTG CCGCTGGGCA CTGGAAATGA CCTGGCGCGT 
GTATTGGGCT GGGGAGGCCT GTGTGACGAC GACGCTCAGC TGCTGCAGAT ACTGGAGAAA 
CTGGAGAGAG CAACAACCAA GATGCTGGAC AGATGGAGTG TGATGACCTA TGAAGTGCCC 
ACCAAACAGA CTCCAGCAAT CGTAAAAGAC GACGACCCAT CAGATTCCCC TCTACAGATT 
ACACAATATG CTGATTCTGT TGCCACTCAC TTGACCAAAA TCCTGGACTC TGACAAACAC 
AGTGACGTTA TTTCATCTGC CAAGTTTCTA TGTGGAACAG TTAATGATTT TGTGGCTGAG 
GTCGGCAAAG CTTATGAACG GGCAACAGAG AACAAGGAGG AGGCCGATGC CATGGCTAAA 
AAGTGTGCCA TGCTGAATGA GAAGCTGGAC TCGTTGGTTA AGGCTCTCAG TGAAGATGCC 
AATGCCCAGC TGGTGCCAGC CGGGTCCATT TCTCCAGACG ACAGCGACAC GGATCAGTCC 
AGACCCTTCA GATCTAGAGA GCAGCTAATG CTGAGGGCCA ACAGCCTGAA GAAAGCACTC 
CGACAAATCA TAGATCAAGC AGAAAAAGTG GTGGATGAGC AGAACAGACA CACACAGGTG 
CAGAGAATGT CTTCCACCTC GTCAATCAAA AGAGGCAGCA GTGAGGACCT CAAGGAGGCG 
GAAACACGTC AAAATACATT GAGTCCGACC GCTACAACTC CTATATTACT GGAGAAGTCA 
GAAAGCTTCG GCACCGTGCT CTTCTCTGAA GAATCTGTGC AGTGCACAGA GAAGTGTGTG 
ATGAATAATT ATTTTGGCAT TGGGCTAGAT GCCAAAATAT CTCTAGAGTT CAACAACAAG 
AGAGACGAGA ACCCCAAAAA ATGCAGTAGT CGCACTAAAA ACATGATGTG GTACGGAGTG 
CTAGGGACCA AAGAACTGGT GCAGAAAACC TACAAGAACC TGGAACAAAG AGTCCAGCTG 
GAGTGTGATG GCGTGTCCAT GTCCTTGCCG AGTCTTCAGG GTCTGGCTGT TTTGAACATC 
CCCAGCTATG CAGGAGGCAT CAATTTCTGG GGAGGCACCA AAGAGGACAA TAATTTTGGA 
GCTCCATCTT TTGATGATAA GAAGCTGGAG GTGGTGGCTG TGTTTGGCAG CATGCAGATG 
GCAATGTCTC GGGTCATCAA CATACAGCAT CACCGCATCG CACAGTGTCG GCAGGTAAAG 
ATCACCATCC TTGGTGATGA AGGTGTTCCT GTGCAGGTGG ATGGTGAGGC CTGGATTCAG 
CCTCCGGGCA TCGTCCAAAT TGTTCACAAG AACCGAGCCC AGATGCTCAC CAGAGACCGG 
GCCTTCGAGA GCACGCTGAA ATCATGGGAG GACAAGCGTA AAGGTGACAG CCGGCCATCT 
CGACCACGAC TCAACTCCCA ACAGTCTATG GAGTATCTGA CGGAGGAGGA ATGTGCTCAG 
GTCCAGCAGC TTGGCTTAGT GGCCGACACG CTCATCAGCA GGATTCGGGA AGTGGCGAAG 
ACTCATAAGG TGGTGGAGCA GGAACTGGCT CATGCTGTAA ATGCCAGCGC TGCTGTCCTG 
AGTGAAGCAT TTCCTTCCAA ACCCTCCAGT CCTGAGGGTT TGAGTCGCAG TACAGCTGTA 
GATATTGTCA ACAGCAGTAC AGTTCTCCAG CAAGAGACCA AGATGCTCCT GGAGGGGAAA 
CTGCTGCAGC TGGAGTCTTC TCAGAAAGAG GAGCTTCTGT CCACAGTGGA GAATCTCAGC 
GCTGAATTAC AGAAGCTGGA GGACATTCAT TGGATCTGTC CCAGCATGCA CTGCTCTGAG 
GAGGAATCAT CCCGTGTGTC TGGAAAAAGC AGCATGAAGC TGAAAATCAT GCCCAAGGGG 
AAGAAAGAGA AGGAAAGGTT ACACAAGCAG CGCTCCAGCA GCTCTCTGTC AGGATCATGG 
GATATCAAAG CCAGCGTATG TGATGTGGAT CCGGCAGGAA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172374.1
CDS	84..3308
Translation	MASKFNPNVLYVREPQESLTSPDPQVEAGDESSDSDGEPEETSHKLIRKVSTSGQIRAKKSVKEGLLLKQTSSFQRWKRRYFKLRGRTLYYAKDSKSLIFDEVDLSDASVAETSTKNINNSFTVITPFRKLMLCAESRKEMEDWISALKSVQKWETYEASQFNMEHFSGMHNWYACSHARPTFCNVCREALPGVTSHGLSCEVCKFKAHKRCAVRSTNTCKWTTLASIGNDIIEDEDGVSMPHQWLEGNLPVSAKCVVCDRNCGSVRRLQDWRCLWCKAIVHNSCKEQLGKVCPLGQCKVSIIPPTALNSIDSDGFWKATSPSCSSPLLVLVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPQLGLRLFQKFETFRTLVCGGDGSVGWVLSELDKLSLHKQCQLGVLPLGTGNDLARVLGWGGLCDDDAQLLQILEKLERATTKMLDRWSVMTYEVPTKQTPAIVKDDDPSDSPLQITQYADSVATHLTKILDSDKHSDVISSAKFLCGTVNDFVAEVGKAYERATENKEEADAMAKKCAMLNEKLDSLVKALSEDANAQLVPAGSISPDDSDTDQSRPFRSREQLMLRANSLKKALRQIIDQAEKVVDEQNRHTQVQRMSSTSSIKRGSSEDLKEAETRQNTLSPTATTPILLEKSESFGTVLFSEESVQCTEKCVMNNYFGIGLDAKISLEFNNKRDENPKKCSSRTKNMMWYGVLGTKELVQKTYKNLEQRVQLECDGVSMSLPSLQGLAVLNIPSYAGGINFWGGTKEDNNFGAPSFDDKKLEVVAVFGSMQMAMSRVINIQHHRIAQCRQVKITILGDEGVPVQVDGEAWIQPPGIVQIVHKNRAQMLTRDRAFESTLKSWEDKRKGDSRPSRPRLNSQQSMEYLTEEECAQVQQLGLVADTLISRIREVAKTHKVVEQELAHAVNASAAVLSEAFPSKPSSPEGLSRSTAVDIVNSSTVLQQETKMLLEGKLLQLESSQKEELLSTVENLSAELQKLEDIHWICPSMHCSEEESSRVSGKSSMKLKIMPKGKKEKERLHKQRSSSSLSGSWDIKASVCDVDPAGN

Target ORF information:

RefSeq Version	XM_005172317.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:dkey-172j4.3 (si:dkey-172j4.3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172317.4

ORF Insert Sequence:

ATGGCCTCAA AGTTCAATCC AAACGTCCTT TATGTCCGGG AGCCCCAGGA GTCCCTGACC 
TCTCCAGACC CTCAAGTTGA GGCGGGTGAC GAATCATCAG ACAGTGACGG GGAGCCTGAA 
GAGACCTCAC ACAAACTCAT CCGAAAAGTG TCCACCTCTG GACAGATTCG AGCCAAGAAG 
AGTGTGAAGG AGGGTCTTCT GCTCAAACAG ACCAGCTCTT TTCAGAGGTG GAAGAGGAGG 
TATTTCAAAC TGCGTGGAAG GACACTTTAT TATGCCAAAG ATTCAAAGTC TCTCATATTT 
GATGAGGTGG ACCTGTCAGA TGCCAGTGTG GCCGAGACCA GCACCAAGAA CATCAACAAC 
AGTTTTACAG TCATCACTCC ATTCCGTAAG CTGATGTTGT GTGCGGAGAG CAGGAAGGAG 
ATGGAAGACT GGATCAGTGC TTTGAAGTCT GTACAGAAAT GGGAAACATA CGAGGCCAGT 
CAGTTCAACA TGGAGCACTT CTCCGGGATG CATAACTGGT ACGCCTGCTC TCATGCCCGG 
CCCACTTTCT GCAACGTGTG TCGAGAGGCT CTGCCGGGTG TTACCTCTCA TGGTCTCTCC 
TGTGAAGTGT GTAAGTTCAA AGCCCACAAG CGCTGTGCCG TACGATCCAC CAATACCTGT 
AAATGGACAA CTCTGGCATC TATAGGAAAT GACATCATCG AGGATGAGGA TGGAGTGTCA 
ATGCCTCACC AGTGGTTGGA GGGAAACCTG CCGGTCAGCG CTAAGTGTGT GGTGTGCGAT 
CGAAACTGCG GCAGCGTCCG GCGACTACAG GACTGGCGGT GTCTCTGGTG CAAAGCCATC 
GTGCACAACA GCTGTAAAGA GCAGTTGGGG AAGGTTTGTC CTCTGGGTCA GTGTAAGGTC 
TCCATCATTC CACCCACTGC ACTTAACAGC ATCGACTCTG ACGGCTTCTG GAAGGCCACC 
AGTCCATCCT GCTCCAGTCC TCTTCTGGTG CTGGTGAACA GTAAGAGTGG AGACAACCAG 
GGTGTCAAGT TCCTGCGCAG GTTTAAGCAG CTCCTGAATC CAGCGCAGGT CTTTGACCTC 
ATGAATGGCG GCCCACAGCT GGGGCTTCGT CTCTTCCAGA AGTTTGAGAC ATTTCGTACC 
CTGGTGTGCG GTGGAGATGG GAGCGTGGGC TGGGTTCTTT CTGAACTGGA CAAACTCAGC 
CTGCACAAAC AGTGTCAGCT GGGTGTGCTG CCGCTGGGCA CTGGAAATGA CCTGGCGCGT 
GTATTGGGCT GGGGAGGCCT GTGTGACGAC GACGCTCAGC TGCTGCAGAT ACTGGAGAAA 
CTGGAGAGAG CAACAACCAA GATGCTGGAC AGATGGAGTG TGATGACCTA TGAAGTGCCC 
ACCAAACAGA CTCCAGCAAT CGTAAAAGAC GACGACCCAT CAGATTCCCC TCTACAGATT 
ACACAATATG CTGATTCTGT TGCCACTCAC TTGACCAAAA TCCTGGACTC TGACAAACAC 
AGTGACGTTA TTTCATCTGC CAAGTTTCTA TGTGGAACAG TTAATGATTT TGTGGCTGAG 
GTCGGCAAAG CTTATGAACG GGCAACAGAG AACAAGGAGG AGGCCGATGC CATGGCTAAA 
AAGTGTGCCA TGCTGAATGA GAAGCTGGAC TCGTTGGTTA AGGCTCTCAG TGAAGATGCC 
AATGCCCAGC TGGTGCCAGC CGGGTCCATT TCTCCAGACG ACAGCGACAC GGATCAGTCC 
AGACCCTTCA GATCTAGAGA GCAGCTAATG CTGAGGGCCA ACAGCCTGAA GAAAGCACTC 
CGACAAATCA TAGATCAAGC AGAAAAAGTG GTGGATGAGC AGAACAGACA CACACAGGTG 
CAGAGAATGT CTTCCACCTC GTCAATCAAA AGAGGCAGCA GTGAGGACCT CAAGGAGGCG 
GAAACACGTC AAAATACATT GAGTCCGACC GCTACAACTC CTATATTACT GGAGAAGTCA 
GAAAGCTTCG GCACCGTGCT CTTCTCTGAA GAATCTGTGC AGTGCACAGA GAAGTGTGTG 
ATGAATAATT ATTTTGGCAT TGGGCTAGAT GCCAAAATAT CTCTAGAGTT CAACAACAAG 
AGAGACGAGA ACCCCAAAAA ATGCAGTAGT CGCACTAAAA ACATGATGTG GTACGGAGTG 
CTAGGGACCA AAGAACTGGT GCAGAAAACC TACAAGAACC TGGAACAAAG AGTCCAGCTG 
GAGTGTGATG GCGTGTCCAT GTCCTTGCCG AGTCTTCAGG GTCTGGCTGT TTTGAACATC 
CCCAGCTATG CAGGAGGCAT CAATTTCTGG GGAGGCACCA AAGAGGACAA TAATTTTGGA 
GCTCCATCTT TTGATGATAA GAAGCTGGAG GTGGTGGCTG TGTTTGGCAG CATGCAGATG 
GCAATGTCTC GGGTCATCAA CATACAGCAT CACCGCATCG CACAGTGTCG GCAGGTAAAG 
ATCACCATCC TTGGTGATGA AGGTGTTCCT GTGCAGGTGG ATGGTGAGGC CTGGATTCAG 
CCTCCGGGCA TCGTCCAAAT TGTTCACAAG AACCGAGCCC AGATGCTCAC CAGAGACCGG 
GCCTTCGAGA GCACGCTGAA ATCATGGGAG GACAAGCGTA AAGGTGACAG CCGGCCATCT 
CGACCACGAC TCAACTCCCA ACAGTCTATG GAGTATCTGA CGGAGGAGGA ATGTGCTCAG 
GTCCAGCAGC TTGGCTTAGT GGCCGACACG CTCATCAGCA GGATTCGGGA AGTGGCGAAG 
ACTCATAAGG TGGTGGAGCA GGAACTGGCT CATGCTGTAA ATGCCAGCGC TGCTGTCCTG 
AGTGAAGCAT TTCCTTCCAA ACCCTCCAGT CCTGAGGGTT TGAGTCGCAG TACAGCTGTA 
GATATTGTCA ACAGCAGTAC AGTTCTCCAG CAAGAGACCA AGATGCTCCT GGAGGGGAAA 
CTGCTGCAGC TGGAGTCTTC TCAGAAAGAG GAGCTTCTGT CCACAGTGGA GAATCTCAGC 
GCTGAATTAC AGAAGCTGGA GGACATTCAT TGGATCTGTC CCAGCATGCA CTGCTCTGAG 
GAGGAATCAT CCCGTGTGTC TGGAAAAAGC AGCATGAAGC TGAAAATCAT GCCCAAGGGG 
AAGAAAGAGA AGGAAAGGTT ACACAAGCAG CGCTCCAGCA GCTCTCTGTC AGGATCATGG 
GATATCAAAG CCAGCGTATG TGATGTGGAT CCGGCAGGAA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:dkey-172j4.3 ( XM_005172316.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172373.1 Danio rerio si:dkey-172j4.3 (si:dkey-172j4.3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa12940
Clone ID Related Accession (Same CDS sequence)	XM_005172317.4 , XM_005172316.3 , XM_021477787.1 , XM_005172317.3 , XM_005172316.4
Accession Version	XM_005172316.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	diacylglycerol kinase kappa isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172316.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAA AGTTCAATCC AAACGTCCTT TATGTCCGGG AGCCCCAGGA GTCCCTGACC 
TCTCCAGACC CTCAAGTTGA GGCGGGTGAC GAATCATCAG ACAGTGACGG GGAGCCTGAA 
GAGACCTCAC ACAAACTCAT CCGAAAAGTG TCCACCTCTG GACAGATTCG AGCCAAGAAG 
AGTGTGAAGG AGGGTCTTCT GCTCAAACAG ACCAGCTCTT TTCAGAGGTG GAAGAGGAGG 
TATTTCAAAC TGCGTGGAAG GACACTTTAT TATGCCAAAG ATTCAAAGTC TCTCATATTT 
GATGAGGTGG ACCTGTCAGA TGCCAGTGTG GCCGAGACCA GCACCAAGAA CATCAACAAC 
AGTTTTACAG TCATCACTCC ATTCCGTAAG CTGATGTTGT GTGCGGAGAG CAGGAAGGAG 
ATGGAAGACT GGATCAGTGC TTTGAAGTCT GTACAGAAAT GGGAAACATA CGAGGCCAGT 
CAGTTCAACA TGGAGCACTT CTCCGGGATG CATAACTGGT ACGCCTGCTC TCATGCCCGG 
CCCACTTTCT GCAACGTGTG TCGAGAGGCT CTGCCGGGTG TTACCTCTCA TGGTCTCTCC 
TGTGAAGTGT GTAAGTTCAA AGCCCACAAG CGCTGTGCCG TACGATCCAC CAATACCTGT 
AAATGGACAA CTCTGGCATC TATAGGAAAT GACATCATCG AGGATGAGGA TGGAGTGTCA 
ATGCCTCACC AGTGGTTGGA GGGAAACCTG CCGGTCAGCG CTAAGTGTGT GGTGTGCGAT 
CGAAACTGCG GCAGCGTCCG GCGACTACAG GACTGGCGGT GTCTCTGGTG CAAAGCCATC 
GTGCACAACA GCTGTAAAGA GCAGTTGGGG AAGGTTTGTC CTCTGGGTCA GTGTAAGGTC 
TCCATCATTC CACCCACTGC ACTTAACAGC ATCGACTCTG ACGGCTTCTG GAAGGCCACC 
AGTCCATCCT GCTCCAGTCC TCTTCTGGTG CTGGTGAACA GTAAGAGTGG AGACAACCAG 
GGTGTCAAGT TCCTGCGCAG GTTTAAGCAG CTCCTGAATC CAGCGCAGGT CTTTGACCTC 
ATGAATGGCG GCCCACAGCT GGGGCTTCGT CTCTTCCAGA AGTTTGAGAC ATTTCGTACC 
CTGGTGTGCG GTGGAGATGG GAGCGTGGGC TGGGTTCTTT CTGAACTGGA CAAACTCAGC 
CTGCACAAAC AGTGTCAGCT GGGTGTGCTG CCGCTGGGCA CTGGAAATGA CCTGGCGCGT 
GTATTGGGCT GGGGAGGCCT GTGTGACGAC GACGCTCAGC TGCTGCAGAT ACTGGAGAAA 
CTGGAGAGAG CAACAACCAA GATGCTGGAC AGATGGAGTG TGATGACCTA TGAAGTGCCC 
ACCAAACAGA CTCCAGCAAT CGTAAAAGAC GACGACCCAT CAGATTCCCC TCTACAGATT 
ACACAATATG CTGATTCTGT TGCCACTCAC TTGACCAAAA TCCTGGACTC TGACAAACAC 
AGTGACGTTA TTTCATCTGC CAAGTTTCTA TGTGGAACAG TTAATGATTT TGTGGCTGAG 
GTCGGCAAAG CTTATGAACG GGCAACAGAG AACAAGGAGG AGGCCGATGC CATGGCTAAA 
AAGTGTGCCA TGCTGAATGA GAAGCTGGAC TCGTTGGTTA AGGCTCTCAG TGAAGATGCC 
AATGCCCAGC TGGTGCCAGC CGGGTCCATT TCTCCAGACG ACAGCGACAC GGATCAGTCC 
AGACCCTTCA GATCTAGAGA GCAGCTAATG CTGAGGGCCA ACAGCCTGAA GAAAGCACTC 
CGACAAATCA TAGATCAAGC AGAAAAAGTG GTGGATGAGC AGAACAGACA CACACAGGTG 
CAGAGAATGT CTTCCACCTC GTCAATCAAA AGAGGCAGCA GTGAGGACCT CAAGGAGGCG 
GAAACACGTC AAAATACATT GAGTCCGACC GCTACAACTC CTATATTACT GGAGAAGTCA 
GAAAGCTTCG GCACCGTGCT CTTCTCTGAA GAATCTGTGC AGTGCACAGA GAAGTGTGTG 
ATGAATAATT ATTTTGGCAT TGGGCTAGAT GCCAAAATAT CTCTAGAGTT CAACAACAAG 
AGAGACGAGA ACCCCAAAAA ATGCAGTAGT CGCACTAAAA ACATGATGTG GTACGGAGTG 
CTAGGGACCA AAGAACTGGT GCAGAAAACC TACAAGAACC TGGAACAAAG AGTCCAGCTG 
GAGTGTGATG GCGTGTCCAT GTCCTTGCCG AGTCTTCAGG GTCTGGCTGT TTTGAACATC 
CCCAGCTATG CAGGAGGCAT CAATTTCTGG GGAGGCACCA AAGAGGACAA TAATTTTGGA 
GCTCCATCTT TTGATGATAA GAAGCTGGAG GTGGTGGCTG TGTTTGGCAG CATGCAGATG 
GCAATGTCTC GGGTCATCAA CATACAGCAT CACCGCATCG CACAGTGTCG GCAGGTAAAG 
ATCACCATCC TTGGTGATGA AGGTGTTCCT GTGCAGGTGG ATGGTGAGGC CTGGATTCAG 
CCTCCGGGCA TCGTCCAAAT TGTTCACAAG AACCGAGCCC AGATGCTCAC CAGAGACCGG 
GCCTTCGAGA GCACGCTGAA ATCATGGGAG GACAAGCGTA AAGGTGACAG CCGGCCATCT 
CGACCACGAC TCAACTCCCA ACAGTCTATG GAGTATCTGA CGGAGGAGGA ATGTGCTCAG 
GTCCAGCAGC TTGGCTTAGT GGCCGACACG CTCATCAGCA GGATTCGGGA AGTGGCGAAG 
ACTCATAAGG TGGTGGAGCA GGAACTGGCT CATGCTGTAA ATGCCAGCGC TGCTGTCCTG 
AGTGAAGCAT TTCCTTCCAA ACCCTCCAGT CCTGAGGGTT TGAGTCGCAG TACAGCTGTA 
GATATTGTCA ACAGCAGTAC AGTTCTCCAG CAAGAGACCA AGATGCTCCT GGAGGGGAAA 
CTGCTGCAGC TGGAGTCTTC TCAGAAAGAG GAGCTTCTGT CCACAGTGGA GAATCTCAGC 
GCTGAATTAC AGAAGCTGGA GGACATTCAT TGGATCTGTC CCAGCATGCA CTGCTCTGAG 
GAGGAATCAT CCCGTGTGTC TGGAAAAAGC AGCATGAAGC TGAAAATCAT GCCCAAGGGG 
AAGAAAGAGA AGGAAAGGTT ACACAAGCAG CGCTCCAGCA GCTCTCTGTC AGGATCATGG 
GATATCAAAG CCAGCGTATG TGATGTGGAT CCGGCAGGAA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172373.1
CDS	235..3459
Translation	MASKFNPNVLYVREPQESLTSPDPQVEAGDESSDSDGEPEETSHKLIRKVSTSGQIRAKKSVKEGLLLKQTSSFQRWKRRYFKLRGRTLYYAKDSKSLIFDEVDLSDASVAETSTKNINNSFTVITPFRKLMLCAESRKEMEDWISALKSVQKWETYEASQFNMEHFSGMHNWYACSHARPTFCNVCREALPGVTSHGLSCEVCKFKAHKRCAVRSTNTCKWTTLASIGNDIIEDEDGVSMPHQWLEGNLPVSAKCVVCDRNCGSVRRLQDWRCLWCKAIVHNSCKEQLGKVCPLGQCKVSIIPPTALNSIDSDGFWKATSPSCSSPLLVLVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPQLGLRLFQKFETFRTLVCGGDGSVGWVLSELDKLSLHKQCQLGVLPLGTGNDLARVLGWGGLCDDDAQLLQILEKLERATTKMLDRWSVMTYEVPTKQTPAIVKDDDPSDSPLQITQYADSVATHLTKILDSDKHSDVISSAKFLCGTVNDFVAEVGKAYERATENKEEADAMAKKCAMLNEKLDSLVKALSEDANAQLVPAGSISPDDSDTDQSRPFRSREQLMLRANSLKKALRQIIDQAEKVVDEQNRHTQVQRMSSTSSIKRGSSEDLKEAETRQNTLSPTATTPILLEKSESFGTVLFSEESVQCTEKCVMNNYFGIGLDAKISLEFNNKRDENPKKCSSRTKNMMWYGVLGTKELVQKTYKNLEQRVQLECDGVSMSLPSLQGLAVLNIPSYAGGINFWGGTKEDNNFGAPSFDDKKLEVVAVFGSMQMAMSRVINIQHHRIAQCRQVKITILGDEGVPVQVDGEAWIQPPGIVQIVHKNRAQMLTRDRAFESTLKSWEDKRKGDSRPSRPRLNSQQSMEYLTEEECAQVQQLGLVADTLISRIREVAKTHKVVEQELAHAVNASAAVLSEAFPSKPSSPEGLSRSTAVDIVNSSTVLQQETKMLLEGKLLQLESSQKEELLSTVENLSAELQKLEDIHWICPSMHCSEEESSRVSGKSSMKLKIMPKGKKEKERLHKQRSSSSLSGSWDIKASVCDVDPAGN

Target ORF information:

RefSeq Version	XM_005172316.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:dkey-172j4.3 (si:dkey-172j4.3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172316.3

ORF Insert Sequence:

ATGGCCTCAA AGTTCAATCC AAACGTCCTT TATGTCCGGG AGCCCCAGGA GTCCCTGACC 
TCTCCAGACC CTCAAGTTGA GGCGGGTGAC GAATCATCAG ACAGTGACGG GGAGCCTGAA 
GAGACCTCAC ACAAACTCAT CCGAAAAGTG TCCACCTCTG GACAGATTCG AGCCAAGAAG 
AGTGTGAAGG AGGGTCTTCT GCTCAAACAG ACCAGCTCTT TTCAGAGGTG GAAGAGGAGG 
TATTTCAAAC TGCGTGGAAG GACACTTTAT TATGCCAAAG ATTCAAAGTC TCTCATATTT 
GATGAGGTGG ACCTGTCAGA TGCCAGTGTG GCCGAGACCA GCACCAAGAA CATCAACAAC 
AGTTTTACAG TCATCACTCC ATTCCGTAAG CTGATGTTGT GTGCGGAGAG CAGGAAGGAG 
ATGGAAGACT GGATCAGTGC TTTGAAGTCT GTACAGAAAT GGGAAACATA CGAGGCCAGT 
CAGTTCAACA TGGAGCACTT CTCCGGGATG CATAACTGGT ACGCCTGCTC TCATGCCCGG 
CCCACTTTCT GCAACGTGTG TCGAGAGGCT CTGCCGGGTG TTACCTCTCA TGGTCTCTCC 
TGTGAAGTGT GTAAGTTCAA AGCCCACAAG CGCTGTGCCG TACGATCCAC CAATACCTGT 
AAATGGACAA CTCTGGCATC TATAGGAAAT GACATCATCG AGGATGAGGA TGGAGTGTCA 
ATGCCTCACC AGTGGTTGGA GGGAAACCTG CCGGTCAGCG CTAAGTGTGT GGTGTGCGAT 
CGAAACTGCG GCAGCGTCCG GCGACTACAG GACTGGCGGT GTCTCTGGTG CAAAGCCATC 
GTGCACAACA GCTGTAAAGA GCAGTTGGGG AAGGTTTGTC CTCTGGGTCA GTGTAAGGTC 
TCCATCATTC CACCCACTGC ACTTAACAGC ATCGACTCTG ACGGCTTCTG GAAGGCCACC 
AGTCCATCCT GCTCCAGTCC TCTTCTGGTG CTGGTGAACA GTAAGAGTGG AGACAACCAG 
GGTGTCAAGT TCCTGCGCAG GTTTAAGCAG CTCCTGAATC CAGCGCAGGT CTTTGACCTC 
ATGAATGGCG GCCCACAGCT GGGGCTTCGT CTCTTCCAGA AGTTTGAGAC ATTTCGTACC 
CTGGTGTGCG GTGGAGATGG GAGCGTGGGC TGGGTTCTTT CTGAACTGGA CAAACTCAGC 
CTGCACAAAC AGTGTCAGCT GGGTGTGCTG CCGCTGGGCA CTGGAAATGA CCTGGCGCGT 
GTATTGGGCT GGGGAGGCCT GTGTGACGAC GACGCTCAGC TGCTGCAGAT ACTGGAGAAA 
CTGGAGAGAG CAACAACCAA GATGCTGGAC AGATGGAGTG TGATGACCTA TGAAGTGCCC 
ACCAAACAGA CTCCAGCAAT CGTAAAAGAC GACGACCCAT CAGATTCCCC TCTACAGATT 
ACACAATATG CTGATTCTGT TGCCACTCAC TTGACCAAAA TCCTGGACTC TGACAAACAC 
AGTGACGTTA TTTCATCTGC CAAGTTTCTA TGTGGAACAG TTAATGATTT TGTGGCTGAG 
GTCGGCAAAG CTTATGAACG GGCAACAGAG AACAAGGAGG AGGCCGATGC CATGGCTAAA 
AAGTGTGCCA TGCTGAATGA GAAGCTGGAC TCGTTGGTTA AGGCTCTCAG TGAAGATGCC 
AATGCCCAGC TGGTGCCAGC CGGGTCCATT TCTCCAGACG ACAGCGACAC GGATCAGTCC 
AGACCCTTCA GATCTAGAGA GCAGCTAATG CTGAGGGCCA ACAGCCTGAA GAAAGCACTC 
CGACAAATCA TAGATCAAGC AGAAAAAGTG GTGGATGAGC AGAACAGACA CACACAGGTG 
CAGAGAATGT CTTCCACCTC GTCAATCAAA AGAGGCAGCA GTGAGGACCT CAAGGAGGCG 
GAAACACGTC AAAATACATT GAGTCCGACC GCTACAACTC CTATATTACT GGAGAAGTCA 
GAAAGCTTCG GCACCGTGCT CTTCTCTGAA GAATCTGTGC AGTGCACAGA GAAGTGTGTG 
ATGAATAATT ATTTTGGCAT TGGGCTAGAT GCCAAAATAT CTCTAGAGTT CAACAACAAG 
AGAGACGAGA ACCCCAAAAA ATGCAGTAGT CGCACTAAAA ACATGATGTG GTACGGAGTG 
CTAGGGACCA AAGAACTGGT GCAGAAAACC TACAAGAACC TGGAACAAAG AGTCCAGCTG 
GAGTGTGATG GCGTGTCCAT GTCCTTGCCG AGTCTTCAGG GTCTGGCTGT TTTGAACATC 
CCCAGCTATG CAGGAGGCAT CAATTTCTGG GGAGGCACCA AAGAGGACAA TAATTTTGGA 
GCTCCATCTT TTGATGATAA GAAGCTGGAG GTGGTGGCTG TGTTTGGCAG CATGCAGATG 
GCAATGTCTC GGGTCATCAA CATACAGCAT CACCGCATCG CACAGTGTCG GCAGGTAAAG 
ATCACCATCC TTGGTGATGA AGGTGTTCCT GTGCAGGTGG ATGGTGAGGC CTGGATTCAG 
CCTCCGGGCA TCGTCCAAAT TGTTCACAAG AACCGAGCCC AGATGCTCAC CAGAGACCGG 
GCCTTCGAGA GCACGCTGAA ATCATGGGAG GACAAGCGTA AAGGTGACAG CCGGCCATCT 
CGACCACGAC TCAACTCCCA ACAGTCTATG GAGTATCTGA CGGAGGAGGA ATGTGCTCAG 
GTCCAGCAGC TTGGCTTAGT GGCCGACACG CTCATCAGCA GGATTCGGGA AGTGGCGAAG 
ACTCATAAGG TGGTGGAGCA GGAACTGGCT CATGCTGTAA ATGCCAGCGC TGCTGTCCTG 
AGTGAAGCAT TTCCTTCCAA ACCCTCCAGT CCTGAGGGTT TGAGTCGCAG TACAGCTGTA 
GATATTGTCA ACAGCAGTAC AGTTCTCCAG CAAGAGACCA AGATGCTCCT GGAGGGGAAA 
CTGCTGCAGC TGGAGTCTTC TCAGAAAGAG GAGCTTCTGT CCACAGTGGA GAATCTCAGC 
GCTGAATTAC AGAAGCTGGA GGACATTCAT TGGATCTGTC CCAGCATGCA CTGCTCTGAG 
GAGGAATCAT CCCGTGTGTC TGGAAAAAGC AGCATGAAGC TGAAAATCAT GCCCAAGGGG 
AAGAAAGAGA AGGAAAGGTT ACACAAGCAG CGCTCCAGCA GCTCTCTGTC AGGATCATGG 
GATATCAAAG CCAGCGTATG TGATGTGGAT CCGGCAGGAA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:dkey-172j4.3 ( XM_021477787.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021333462.1 Danio rerio si:dkey-172j4.3 (si:dkey-172j4.3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa12940
Clone ID Related Accession (Same CDS sequence)	XM_005172317.4 , XM_005172316.3 , XM_021477787.1 , XM_005172317.3 , XM_005172316.4
Accession Version	XM_021477787.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	diacylglycerol kinase delta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAA AGTTCAATCC AAACGTCCTT TATGTCCGGG AGCCCCAGGA GTCCCTGACC 
TCTCCAGACC CTCAAGTTGA GGCGGGTGAC GAATCATCAG ACAGTGACGG GGAGCCTGAA 
GAGACCTCAC ACAAACTCAT CCGAAAAGTG TCCACCTCTG GACAGATTCG AGCCAAGAAG 
AGTGTGAAGG AGGGTCTTCT GCTCAAACAG ACCAGCTCTT TTCAGAGGTG GAAGAGGAGG 
TATTTCAAAC TGCGTGGAAG GACACTTTAT TATGCCAAAG ATTCAAAGTC TCTCATATTT 
GATGAGGTGG ACCTGTCAGA TGCCAGTGTG GCCGAGACCA GCACCAAGAA CATCAACAAC 
AGTTTTACAG TCATCACTCC ATTCCGTAAG CTGATGTTGT GTGCGGAGAG CAGGAAGGAG 
ATGGAAGACT GGATCAGTGC TTTGAAGTCT GTACAGAAAT GGGAAACATA CGAGGCCAGT 
CAGTTCAACA TGGAGCACTT CTCCGGGATG CATAACTGGT ACGCCTGCTC TCATGCCCGG 
CCCACTTTCT GCAACGTGTG TCGAGAGGCT CTGCCGGGTG TTACCTCTCA TGGTCTCTCC 
TGTGAAGTGT GTAAGTTCAA AGCCCACAAG CGCTGTGCCG TACGATCCAC CAATACCTGT 
AAATGGACAA CTCTGGCATC TATAGGAAAT GACATCATCG AGGATGAGGA TGGAGTGTCA 
ATGCCTCACC AGTGGTTGGA GGGAAACCTG CCGGTCAGCG CTAAGTGTGT GGTGTGCGAT 
CGAAACTGCG GCAGCGTCCG GCGACTACAG GACTGGCGGT GTCTCTGGTG CAAAGCCATC 
GTGCACAACA GCTGTAAAGA GCAGTTGGGG AAGGTTTGTC CTCTGGGTCA GTGTAAGGTC 
TCCATCATTC CACCCACTGC ACTTAACAGC ATCGACTCTG ACGGCTTCTG GAAGGCCACC 
AGTCCATCCT GCTCCAGTCC TCTTCTGGTG CTGGTGAACA GTAAGAGTGG AGACAACCAG 
GGTGTCAAGT TCCTGCGCAG GTTTAAGCAG CTCCTGAATC CAGCGCAGGT CTTTGACCTC 
ATGAATGGCG GCCCACAGCT GGGGCTTCGT CTCTTCCAGA AGTTTGAGAC ATTTCGTACC 
CTGGTGTGCG GTGGAGATGG GAGCGTGGGC TGGGTTCTTT CTGAACTGGA CAAACTCAGC 
CTGCACAAAC AGTGTCAGCT GGGTGTGCTG CCGCTGGGCA CTGGAAATGA CCTGGCGCGT 
GTATTGGGCT GGGGAGGCCT GTGTGACGAC GACGCTCAGC TGCTGCAGAT ACTGGAGAAA 
CTGGAGAGAG CAACAACCAA GATGCTGGAC AGATGGAGTG TGATGACCTA TGAAGTGCCC 
ACCAAACAGA CTCCAGCAAT CGTAAAAGAC GACGACCCAT CAGATTCCCC TCTACAGATT 
ACACAATATG CTGATTCTGT TGCCACTCAC TTGACCAAAA TCCTGGACTC TGACAAACAC 
AGTGACGTTA TTTCATCTGC CAAGTTTCTA TGTGGAACAG TTAATGATTT TGTGGCTGAG 
GTCGGCAAAG CTTATGAACG GGCAACAGAG AACAAGGAGG AGGCCGATGC CATGGCTAAA 
AAGTGTGCCA TGCTGAATGA GAAGCTGGAC TCGTTGGTTA AGGCTCTCAG TGAAGATGCC 
AATGCCCAGC TGGTGCCAGC CGGGTCCATT TCTCCAGACG ACAGCGACAC GGATCAGTCC 
AGACCCTTCA GATCTAGAGA GCAGCTAATG CTGAGGGCCA ACAGCCTGAA GAAAGCACTC 
CGACAAATCA TAGATCAAGC AGAAAAAGTG GTGGATGAGC AGAACAGACA CACACAGGTG 
CAGAGAATGT CTTCCACCTC GTCAATCAAA AGAGGCAGCA GTGAGGACCT CAAGGAGGCG 
GAAACACGTC AAAATACATT GAGTCCGACC GCTACAACTC CTATATTACT GGAGAAGTCA 
GAAAGCTTCG GCACCGTGCT CTTCTCTGAA GAATCTGTGC AGTGCACAGA GAAGTGTGTG 
ATGAATAATT ATTTTGGCAT TGGGCTAGAT GCCAAAATAT CTCTAGAGTT CAACAACAAG 
AGAGACGAGA ACCCCAAAAA ATGCAGTAGT CGCACTAAAA ACATGATGTG GTACGGAGTG 
CTAGGGACCA AAGAACTGGT GCAGAAAACC TACAAGAACC TGGAACAAAG AGTCCAGCTG 
GAGTGTGATG GCGTGTCCAT GTCCTTGCCG AGTCTTCAGG GTCTGGCTGT TTTGAACATC 
CCCAGCTATG CAGGAGGCAT CAATTTCTGG GGAGGCACCA AAGAGGACAA TAATTTTGGA 
GCTCCATCTT TTGATGATAA GAAGCTGGAG GTGGTGGCTG TGTTTGGCAG CATGCAGATG 
GCAATGTCTC GGGTCATCAA CATACAGCAT CACCGCATCG CACAGTGTCG GCAGGTAAAG 
ATCACCATCC TTGGTGATGA AGGTGTTCCT GTGCAGGTGG ATGGTGAGGC CTGGATTCAG 
CCTCCGGGCA TCGTCCAAAT TGTTCACAAG AACCGAGCCC AGATGCTCAC CAGAGACCGG 
GCCTTCGAGA GCACGCTGAA ATCATGGGAG GACAAGCGTA AAGGTGACAG CCGGCCATCT 
CGACCACGAC TCAACTCCCA ACAGTCTATG GAGTATCTGA CGGAGGAGGA ATGTGCTCAG 
GTCCAGCAGC TTGGCTTAGT GGCCGACACG CTCATCAGCA GGATTCGGGA AGTGGCGAAG 
ACTCATAAGG TGGTGGAGCA GGAACTGGCT CATGCTGTAA ATGCCAGCGC TGCTGTCCTG 
AGTGAAGCAT TTCCTTCCAA ACCCTCCAGT CCTGAGGGTT TGAGTCGCAG TACAGCTGTA 
GATATTGTCA ACAGCAGTAC AGTTCTCCAG CAAGAGACCA AGATGCTCCT GGAGGGGAAA 
CTGCTGCAGC TGGAGTCTTC TCAGAAAGAG GAGCTTCTGT CCACAGTGGA GAATCTCAGC 
GCTGAATTAC AGAAGCTGGA GGACATTCAT TGGATCTGTC CCAGCATGCA CTGCTCTGAG 
GAGGAATCAT CCCGTGTGTC TGGAAAAAGC AGCATGAAGC TGAAAATCAT GCCCAAGGGG 
AAGAAAGAGA AGGAAAGGTT ACACAAGCAG CGCTCCAGCA GCTCTCTGTC AGGATCATGG 
GATATCAAAG CCAGCGTATG TGATGTGGAT CCGGCAGGAA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021333462.1
CDS	78..3302
Translation	MASKFNPNVLYVREPQESLTSPDPQVEAGDESSDSDGEPEETSHKLIRKVSTSGQIRAKKSVKEGLLLKQTSSFQRWKRRYFKLRGRTLYYAKDSKSLIFDEVDLSDASVAETSTKNINNSFTVITPFRKLMLCAESRKEMEDWISALKSVQKWETYEASQFNMEHFSGMHNWYACSHARPTFCNVCREALPGVTSHGLSCEVCKFKAHKRCAVRSTNTCKWTTLASIGNDIIEDEDGVSMPHQWLEGNLPVSAKCVVCDRNCGSVRRLQDWRCLWCKAIVHNSCKEQLGKVCPLGQCKVSIIPPTALNSIDSDGFWKATSPSCSSPLLVLVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPQLGLRLFQKFETFRTLVCGGDGSVGWVLSELDKLSLHKQCQLGVLPLGTGNDLARVLGWGGLCDDDAQLLQILEKLERATTKMLDRWSVMTYEVPTKQTPAIVKDDDPSDSPLQITQYADSVATHLTKILDSDKHSDVISSAKFLCGTVNDFVAEVGKAYERATENKEEADAMAKKCAMLNEKLDSLVKALSEDANAQLVPAGSISPDDSDTDQSRPFRSREQLMLRANSLKKALRQIIDQAEKVVDEQNRHTQVQRMSSTSSIKRGSSEDLKEAETRQNTLSPTATTPILLEKSESFGTVLFSEESVQCTEKCVMNNYFGIGLDAKISLEFNNKRDENPKKCSSRTKNMMWYGVLGTKELVQKTYKNLEQRVQLECDGVSMSLPSLQGLAVLNIPSYAGGINFWGGTKEDNNFGAPSFDDKKLEVVAVFGSMQMAMSRVINIQHHRIAQCRQVKITILGDEGVPVQVDGEAWIQPPGIVQIVHKNRAQMLTRDRAFESTLKSWEDKRKGDSRPSRPRLNSQQSMEYLTEEECAQVQQLGLVADTLISRIREVAKTHKVVEQELAHAVNASAAVLSEAFPSKPSSPEGLSRSTAVDIVNSSTVLQQETKMLLEGKLLQLESSQKEELLSTVENLSAELQKLEDIHWICPSMHCSEEESSRVSGKSSMKLKIMPKGKKEKERLHKQRSSSSLSGSWDIKASVCDVDPAGN

Target ORF information:

RefSeq Version	XM_021477787.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:dkey-172j4.3 (si:dkey-172j4.3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477787.1

ORF Insert Sequence:

ATGGCCTCAA AGTTCAATCC AAACGTCCTT TATGTCCGGG AGCCCCAGGA GTCCCTGACC 
TCTCCAGACC CTCAAGTTGA GGCGGGTGAC GAATCATCAG ACAGTGACGG GGAGCCTGAA 
GAGACCTCAC ACAAACTCAT CCGAAAAGTG TCCACCTCTG GACAGATTCG AGCCAAGAAG 
AGTGTGAAGG AGGGTCTTCT GCTCAAACAG ACCAGCTCTT TTCAGAGGTG GAAGAGGAGG 
TATTTCAAAC TGCGTGGAAG GACACTTTAT TATGCCAAAG ATTCAAAGTC TCTCATATTT 
GATGAGGTGG ACCTGTCAGA TGCCAGTGTG GCCGAGACCA GCACCAAGAA CATCAACAAC 
AGTTTTACAG TCATCACTCC ATTCCGTAAG CTGATGTTGT GTGCGGAGAG CAGGAAGGAG 
ATGGAAGACT GGATCAGTGC TTTGAAGTCT GTACAGAAAT GGGAAACATA CGAGGCCAGT 
CAGTTCAACA TGGAGCACTT CTCCGGGATG CATAACTGGT ACGCCTGCTC TCATGCCCGG 
CCCACTTTCT GCAACGTGTG TCGAGAGGCT CTGCCGGGTG TTACCTCTCA TGGTCTCTCC 
TGTGAAGTGT GTAAGTTCAA AGCCCACAAG CGCTGTGCCG TACGATCCAC CAATACCTGT 
AAATGGACAA CTCTGGCATC TATAGGAAAT GACATCATCG AGGATGAGGA TGGAGTGTCA 
ATGCCTCACC AGTGGTTGGA GGGAAACCTG CCGGTCAGCG CTAAGTGTGT GGTGTGCGAT 
CGAAACTGCG GCAGCGTCCG GCGACTACAG GACTGGCGGT GTCTCTGGTG CAAAGCCATC 
GTGCACAACA GCTGTAAAGA GCAGTTGGGG AAGGTTTGTC CTCTGGGTCA GTGTAAGGTC 
TCCATCATTC CACCCACTGC ACTTAACAGC ATCGACTCTG ACGGCTTCTG GAAGGCCACC 
AGTCCATCCT GCTCCAGTCC TCTTCTGGTG CTGGTGAACA GTAAGAGTGG AGACAACCAG 
GGTGTCAAGT TCCTGCGCAG GTTTAAGCAG CTCCTGAATC CAGCGCAGGT CTTTGACCTC 
ATGAATGGCG GCCCACAGCT GGGGCTTCGT CTCTTCCAGA AGTTTGAGAC ATTTCGTACC 
CTGGTGTGCG GTGGAGATGG GAGCGTGGGC TGGGTTCTTT CTGAACTGGA CAAACTCAGC 
CTGCACAAAC AGTGTCAGCT GGGTGTGCTG CCGCTGGGCA CTGGAAATGA CCTGGCGCGT 
GTATTGGGCT GGGGAGGCCT GTGTGACGAC GACGCTCAGC TGCTGCAGAT ACTGGAGAAA 
CTGGAGAGAG CAACAACCAA GATGCTGGAC AGATGGAGTG TGATGACCTA TGAAGTGCCC 
ACCAAACAGA CTCCAGCAAT CGTAAAAGAC GACGACCCAT CAGATTCCCC TCTACAGATT 
ACACAATATG CTGATTCTGT TGCCACTCAC TTGACCAAAA TCCTGGACTC TGACAAACAC 
AGTGACGTTA TTTCATCTGC CAAGTTTCTA TGTGGAACAG TTAATGATTT TGTGGCTGAG 
GTCGGCAAAG CTTATGAACG GGCAACAGAG AACAAGGAGG AGGCCGATGC CATGGCTAAA 
AAGTGTGCCA TGCTGAATGA GAAGCTGGAC TCGTTGGTTA AGGCTCTCAG TGAAGATGCC 
AATGCCCAGC TGGTGCCAGC CGGGTCCATT TCTCCAGACG ACAGCGACAC GGATCAGTCC 
AGACCCTTCA GATCTAGAGA GCAGCTAATG CTGAGGGCCA ACAGCCTGAA GAAAGCACTC 
CGACAAATCA TAGATCAAGC AGAAAAAGTG GTGGATGAGC AGAACAGACA CACACAGGTG 
CAGAGAATGT CTTCCACCTC GTCAATCAAA AGAGGCAGCA GTGAGGACCT CAAGGAGGCG 
GAAACACGTC AAAATACATT GAGTCCGACC GCTACAACTC CTATATTACT GGAGAAGTCA 
GAAAGCTTCG GCACCGTGCT CTTCTCTGAA GAATCTGTGC AGTGCACAGA GAAGTGTGTG 
ATGAATAATT ATTTTGGCAT TGGGCTAGAT GCCAAAATAT CTCTAGAGTT CAACAACAAG 
AGAGACGAGA ACCCCAAAAA ATGCAGTAGT CGCACTAAAA ACATGATGTG GTACGGAGTG 
CTAGGGACCA AAGAACTGGT GCAGAAAACC TACAAGAACC TGGAACAAAG AGTCCAGCTG 
GAGTGTGATG GCGTGTCCAT GTCCTTGCCG AGTCTTCAGG GTCTGGCTGT TTTGAACATC 
CCCAGCTATG CAGGAGGCAT CAATTTCTGG GGAGGCACCA AAGAGGACAA TAATTTTGGA 
GCTCCATCTT TTGATGATAA GAAGCTGGAG GTGGTGGCTG TGTTTGGCAG CATGCAGATG 
GCAATGTCTC GGGTCATCAA CATACAGCAT CACCGCATCG CACAGTGTCG GCAGGTAAAG 
ATCACCATCC TTGGTGATGA AGGTGTTCCT GTGCAGGTGG ATGGTGAGGC CTGGATTCAG 
CCTCCGGGCA TCGTCCAAAT TGTTCACAAG AACCGAGCCC AGATGCTCAC CAGAGACCGG 
GCCTTCGAGA GCACGCTGAA ATCATGGGAG GACAAGCGTA AAGGTGACAG CCGGCCATCT 
CGACCACGAC TCAACTCCCA ACAGTCTATG GAGTATCTGA CGGAGGAGGA ATGTGCTCAG 
GTCCAGCAGC TTGGCTTAGT GGCCGACACG CTCATCAGCA GGATTCGGGA AGTGGCGAAG 
ACTCATAAGG TGGTGGAGCA GGAACTGGCT CATGCTGTAA ATGCCAGCGC TGCTGTCCTG 
AGTGAAGCAT TTCCTTCCAA ACCCTCCAGT CCTGAGGGTT TGAGTCGCAG TACAGCTGTA 
GATATTGTCA ACAGCAGTAC AGTTCTCCAG CAAGAGACCA AGATGCTCCT GGAGGGGAAA 
CTGCTGCAGC TGGAGTCTTC TCAGAAAGAG GAGCTTCTGT CCACAGTGGA GAATCTCAGC 
GCTGAATTAC AGAAGCTGGA GGACATTCAT TGGATCTGTC CCAGCATGCA CTGCTCTGAG 
GAGGAATCAT CCCGTGTGTC TGGAAAAAGC AGCATGAAGC TGAAAATCAT GCCCAAGGGG 
AAGAAAGAGA AGGAAAGGTT ACACAAGCAG CGCTCCAGCA GCTCTCTGTC AGGATCATGG 
GATATCAAAG CCAGCGTATG TGATGTGGAT CCGGCAGGAA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:dkey-172j4.3 ( XM_005172317.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172374.1 Danio rerio si:dkey-172j4.3 (si:dkey-172j4.3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa12940
Clone ID Related Accession (Same CDS sequence)	XM_005172317.4 , XM_005172316.3 , XM_021477787.1 , XM_005172317.3 , XM_005172316.4
Accession Version	XM_005172317.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	diacylglycerol kinase kappa isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172317.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAA AGTTCAATCC AAACGTCCTT TATGTCCGGG AGCCCCAGGA GTCCCTGACC 
TCTCCAGACC CTCAAGTTGA GGCGGGTGAC GAATCATCAG ACAGTGACGG GGAGCCTGAA 
GAGACCTCAC ACAAACTCAT CCGAAAAGTG TCCACCTCTG GACAGATTCG AGCCAAGAAG 
AGTGTGAAGG AGGGTCTTCT GCTCAAACAG ACCAGCTCTT TTCAGAGGTG GAAGAGGAGG 
TATTTCAAAC TGCGTGGAAG GACACTTTAT TATGCCAAAG ATTCAAAGTC TCTCATATTT 
GATGAGGTGG ACCTGTCAGA TGCCAGTGTG GCCGAGACCA GCACCAAGAA CATCAACAAC 
AGTTTTACAG TCATCACTCC ATTCCGTAAG CTGATGTTGT GTGCGGAGAG CAGGAAGGAG 
ATGGAAGACT GGATCAGTGC TTTGAAGTCT GTACAGAAAT GGGAAACATA CGAGGCCAGT 
CAGTTCAACA TGGAGCACTT CTCCGGGATG CATAACTGGT ACGCCTGCTC TCATGCCCGG 
CCCACTTTCT GCAACGTGTG TCGAGAGGCT CTGCCGGGTG TTACCTCTCA TGGTCTCTCC 
TGTGAAGTGT GTAAGTTCAA AGCCCACAAG CGCTGTGCCG TACGATCCAC CAATACCTGT 
AAATGGACAA CTCTGGCATC TATAGGAAAT GACATCATCG AGGATGAGGA TGGAGTGTCA 
ATGCCTCACC AGTGGTTGGA GGGAAACCTG CCGGTCAGCG CTAAGTGTGT GGTGTGCGAT 
CGAAACTGCG GCAGCGTCCG GCGACTACAG GACTGGCGGT GTCTCTGGTG CAAAGCCATC 
GTGCACAACA GCTGTAAAGA GCAGTTGGGG AAGGTTTGTC CTCTGGGTCA GTGTAAGGTC 
TCCATCATTC CACCCACTGC ACTTAACAGC ATCGACTCTG ACGGCTTCTG GAAGGCCACC 
AGTCCATCCT GCTCCAGTCC TCTTCTGGTG CTGGTGAACA GTAAGAGTGG AGACAACCAG 
GGTGTCAAGT TCCTGCGCAG GTTTAAGCAG CTCCTGAATC CAGCGCAGGT CTTTGACCTC 
ATGAATGGCG GCCCACAGCT GGGGCTTCGT CTCTTCCAGA AGTTTGAGAC ATTTCGTACC 
CTGGTGTGCG GTGGAGATGG GAGCGTGGGC TGGGTTCTTT CTGAACTGGA CAAACTCAGC 
CTGCACAAAC AGTGTCAGCT GGGTGTGCTG CCGCTGGGCA CTGGAAATGA CCTGGCGCGT 
GTATTGGGCT GGGGAGGCCT GTGTGACGAC GACGCTCAGC TGCTGCAGAT ACTGGAGAAA 
CTGGAGAGAG CAACAACCAA GATGCTGGAC AGATGGAGTG TGATGACCTA TGAAGTGCCC 
ACCAAACAGA CTCCAGCAAT CGTAAAAGAC GACGACCCAT CAGATTCCCC TCTACAGATT 
ACACAATATG CTGATTCTGT TGCCACTCAC TTGACCAAAA TCCTGGACTC TGACAAACAC 
AGTGACGTTA TTTCATCTGC CAAGTTTCTA TGTGGAACAG TTAATGATTT TGTGGCTGAG 
GTCGGCAAAG CTTATGAACG GGCAACAGAG AACAAGGAGG AGGCCGATGC CATGGCTAAA 
AAGTGTGCCA TGCTGAATGA GAAGCTGGAC TCGTTGGTTA AGGCTCTCAG TGAAGATGCC 
AATGCCCAGC TGGTGCCAGC CGGGTCCATT TCTCCAGACG ACAGCGACAC GGATCAGTCC 
AGACCCTTCA GATCTAGAGA GCAGCTAATG CTGAGGGCCA ACAGCCTGAA GAAAGCACTC 
CGACAAATCA TAGATCAAGC AGAAAAAGTG GTGGATGAGC AGAACAGACA CACACAGGTG 
CAGAGAATGT CTTCCACCTC GTCAATCAAA AGAGGCAGCA GTGAGGACCT CAAGGAGGCG 
GAAACACGTC AAAATACATT GAGTCCGACC GCTACAACTC CTATATTACT GGAGAAGTCA 
GAAAGCTTCG GCACCGTGCT CTTCTCTGAA GAATCTGTGC AGTGCACAGA GAAGTGTGTG 
ATGAATAATT ATTTTGGCAT TGGGCTAGAT GCCAAAATAT CTCTAGAGTT CAACAACAAG 
AGAGACGAGA ACCCCAAAAA ATGCAGTAGT CGCACTAAAA ACATGATGTG GTACGGAGTG 
CTAGGGACCA AAGAACTGGT GCAGAAAACC TACAAGAACC TGGAACAAAG AGTCCAGCTG 
GAGTGTGATG GCGTGTCCAT GTCCTTGCCG AGTCTTCAGG GTCTGGCTGT TTTGAACATC 
CCCAGCTATG CAGGAGGCAT CAATTTCTGG GGAGGCACCA AAGAGGACAA TAATTTTGGA 
GCTCCATCTT TTGATGATAA GAAGCTGGAG GTGGTGGCTG TGTTTGGCAG CATGCAGATG 
GCAATGTCTC GGGTCATCAA CATACAGCAT CACCGCATCG CACAGTGTCG GCAGGTAAAG 
ATCACCATCC TTGGTGATGA AGGTGTTCCT GTGCAGGTGG ATGGTGAGGC CTGGATTCAG 
CCTCCGGGCA TCGTCCAAAT TGTTCACAAG AACCGAGCCC AGATGCTCAC CAGAGACCGG 
GCCTTCGAGA GCACGCTGAA ATCATGGGAG GACAAGCGTA AAGGTGACAG CCGGCCATCT 
CGACCACGAC TCAACTCCCA ACAGTCTATG GAGTATCTGA CGGAGGAGGA ATGTGCTCAG 
GTCCAGCAGC TTGGCTTAGT GGCCGACACG CTCATCAGCA GGATTCGGGA AGTGGCGAAG 
ACTCATAAGG TGGTGGAGCA GGAACTGGCT CATGCTGTAA ATGCCAGCGC TGCTGTCCTG 
AGTGAAGCAT TTCCTTCCAA ACCCTCCAGT CCTGAGGGTT TGAGTCGCAG TACAGCTGTA 
GATATTGTCA ACAGCAGTAC AGTTCTCCAG CAAGAGACCA AGATGCTCCT GGAGGGGAAA 
CTGCTGCAGC TGGAGTCTTC TCAGAAAGAG GAGCTTCTGT CCACAGTGGA GAATCTCAGC 
GCTGAATTAC AGAAGCTGGA GGACATTCAT TGGATCTGTC CCAGCATGCA CTGCTCTGAG 
GAGGAATCAT CCCGTGTGTC TGGAAAAAGC AGCATGAAGC TGAAAATCAT GCCCAAGGGG 
AAGAAAGAGA AGGAAAGGTT ACACAAGCAG CGCTCCAGCA GCTCTCTGTC AGGATCATGG 
GATATCAAAG CCAGCGTATG TGATGTGGAT CCGGCAGGAA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172374.1
CDS	158..3382
Translation	MASKFNPNVLYVREPQESLTSPDPQVEAGDESSDSDGEPEETSHKLIRKVSTSGQIRAKKSVKEGLLLKQTSSFQRWKRRYFKLRGRTLYYAKDSKSLIFDEVDLSDASVAETSTKNINNSFTVITPFRKLMLCAESRKEMEDWISALKSVQKWETYEASQFNMEHFSGMHNWYACSHARPTFCNVCREALPGVTSHGLSCEVCKFKAHKRCAVRSTNTCKWTTLASIGNDIIEDEDGVSMPHQWLEGNLPVSAKCVVCDRNCGSVRRLQDWRCLWCKAIVHNSCKEQLGKVCPLGQCKVSIIPPTALNSIDSDGFWKATSPSCSSPLLVLVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPQLGLRLFQKFETFRTLVCGGDGSVGWVLSELDKLSLHKQCQLGVLPLGTGNDLARVLGWGGLCDDDAQLLQILEKLERATTKMLDRWSVMTYEVPTKQTPAIVKDDDPSDSPLQITQYADSVATHLTKILDSDKHSDVISSAKFLCGTVNDFVAEVGKAYERATENKEEADAMAKKCAMLNEKLDSLVKALSEDANAQLVPAGSISPDDSDTDQSRPFRSREQLMLRANSLKKALRQIIDQAEKVVDEQNRHTQVQRMSSTSSIKRGSSEDLKEAETRQNTLSPTATTPILLEKSESFGTVLFSEESVQCTEKCVMNNYFGIGLDAKISLEFNNKRDENPKKCSSRTKNMMWYGVLGTKELVQKTYKNLEQRVQLECDGVSMSLPSLQGLAVLNIPSYAGGINFWGGTKEDNNFGAPSFDDKKLEVVAVFGSMQMAMSRVINIQHHRIAQCRQVKITILGDEGVPVQVDGEAWIQPPGIVQIVHKNRAQMLTRDRAFESTLKSWEDKRKGDSRPSRPRLNSQQSMEYLTEEECAQVQQLGLVADTLISRIREVAKTHKVVEQELAHAVNASAAVLSEAFPSKPSSPEGLSRSTAVDIVNSSTVLQQETKMLLEGKLLQLESSQKEELLSTVENLSAELQKLEDIHWICPSMHCSEEESSRVSGKSSMKLKIMPKGKKEKERLHKQRSSSSLSGSWDIKASVCDVDPAGN

Target ORF information:

RefSeq Version	XM_005172317.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:dkey-172j4.3 (si:dkey-172j4.3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172317.3

ORF Insert Sequence:

ATGGCCTCAA AGTTCAATCC AAACGTCCTT TATGTCCGGG AGCCCCAGGA GTCCCTGACC 
TCTCCAGACC CTCAAGTTGA GGCGGGTGAC GAATCATCAG ACAGTGACGG GGAGCCTGAA 
GAGACCTCAC ACAAACTCAT CCGAAAAGTG TCCACCTCTG GACAGATTCG AGCCAAGAAG 
AGTGTGAAGG AGGGTCTTCT GCTCAAACAG ACCAGCTCTT TTCAGAGGTG GAAGAGGAGG 
TATTTCAAAC TGCGTGGAAG GACACTTTAT TATGCCAAAG ATTCAAAGTC TCTCATATTT 
GATGAGGTGG ACCTGTCAGA TGCCAGTGTG GCCGAGACCA GCACCAAGAA CATCAACAAC 
AGTTTTACAG TCATCACTCC ATTCCGTAAG CTGATGTTGT GTGCGGAGAG CAGGAAGGAG 
ATGGAAGACT GGATCAGTGC TTTGAAGTCT GTACAGAAAT GGGAAACATA CGAGGCCAGT 
CAGTTCAACA TGGAGCACTT CTCCGGGATG CATAACTGGT ACGCCTGCTC TCATGCCCGG 
CCCACTTTCT GCAACGTGTG TCGAGAGGCT CTGCCGGGTG TTACCTCTCA TGGTCTCTCC 
TGTGAAGTGT GTAAGTTCAA AGCCCACAAG CGCTGTGCCG TACGATCCAC CAATACCTGT 
AAATGGACAA CTCTGGCATC TATAGGAAAT GACATCATCG AGGATGAGGA TGGAGTGTCA 
ATGCCTCACC AGTGGTTGGA GGGAAACCTG CCGGTCAGCG CTAAGTGTGT GGTGTGCGAT 
CGAAACTGCG GCAGCGTCCG GCGACTACAG GACTGGCGGT GTCTCTGGTG CAAAGCCATC 
GTGCACAACA GCTGTAAAGA GCAGTTGGGG AAGGTTTGTC CTCTGGGTCA GTGTAAGGTC 
TCCATCATTC CACCCACTGC ACTTAACAGC ATCGACTCTG ACGGCTTCTG GAAGGCCACC 
AGTCCATCCT GCTCCAGTCC TCTTCTGGTG CTGGTGAACA GTAAGAGTGG AGACAACCAG 
GGTGTCAAGT TCCTGCGCAG GTTTAAGCAG CTCCTGAATC CAGCGCAGGT CTTTGACCTC 
ATGAATGGCG GCCCACAGCT GGGGCTTCGT CTCTTCCAGA AGTTTGAGAC ATTTCGTACC 
CTGGTGTGCG GTGGAGATGG GAGCGTGGGC TGGGTTCTTT CTGAACTGGA CAAACTCAGC 
CTGCACAAAC AGTGTCAGCT GGGTGTGCTG CCGCTGGGCA CTGGAAATGA CCTGGCGCGT 
GTATTGGGCT GGGGAGGCCT GTGTGACGAC GACGCTCAGC TGCTGCAGAT ACTGGAGAAA 
CTGGAGAGAG CAACAACCAA GATGCTGGAC AGATGGAGTG TGATGACCTA TGAAGTGCCC 
ACCAAACAGA CTCCAGCAAT CGTAAAAGAC GACGACCCAT CAGATTCCCC TCTACAGATT 
ACACAATATG CTGATTCTGT TGCCACTCAC TTGACCAAAA TCCTGGACTC TGACAAACAC 
AGTGACGTTA TTTCATCTGC CAAGTTTCTA TGTGGAACAG TTAATGATTT TGTGGCTGAG 
GTCGGCAAAG CTTATGAACG GGCAACAGAG AACAAGGAGG AGGCCGATGC CATGGCTAAA 
AAGTGTGCCA TGCTGAATGA GAAGCTGGAC TCGTTGGTTA AGGCTCTCAG TGAAGATGCC 
AATGCCCAGC TGGTGCCAGC CGGGTCCATT TCTCCAGACG ACAGCGACAC GGATCAGTCC 
AGACCCTTCA GATCTAGAGA GCAGCTAATG CTGAGGGCCA ACAGCCTGAA GAAAGCACTC 
CGACAAATCA TAGATCAAGC AGAAAAAGTG GTGGATGAGC AGAACAGACA CACACAGGTG 
CAGAGAATGT CTTCCACCTC GTCAATCAAA AGAGGCAGCA GTGAGGACCT CAAGGAGGCG 
GAAACACGTC AAAATACATT GAGTCCGACC GCTACAACTC CTATATTACT GGAGAAGTCA 
GAAAGCTTCG GCACCGTGCT CTTCTCTGAA GAATCTGTGC AGTGCACAGA GAAGTGTGTG 
ATGAATAATT ATTTTGGCAT TGGGCTAGAT GCCAAAATAT CTCTAGAGTT CAACAACAAG 
AGAGACGAGA ACCCCAAAAA ATGCAGTAGT CGCACTAAAA ACATGATGTG GTACGGAGTG 
CTAGGGACCA AAGAACTGGT GCAGAAAACC TACAAGAACC TGGAACAAAG AGTCCAGCTG 
GAGTGTGATG GCGTGTCCAT GTCCTTGCCG AGTCTTCAGG GTCTGGCTGT TTTGAACATC 
CCCAGCTATG CAGGAGGCAT CAATTTCTGG GGAGGCACCA AAGAGGACAA TAATTTTGGA 
GCTCCATCTT TTGATGATAA GAAGCTGGAG GTGGTGGCTG TGTTTGGCAG CATGCAGATG 
GCAATGTCTC GGGTCATCAA CATACAGCAT CACCGCATCG CACAGTGTCG GCAGGTAAAG 
ATCACCATCC TTGGTGATGA AGGTGTTCCT GTGCAGGTGG ATGGTGAGGC CTGGATTCAG 
CCTCCGGGCA TCGTCCAAAT TGTTCACAAG AACCGAGCCC AGATGCTCAC CAGAGACCGG 
GCCTTCGAGA GCACGCTGAA ATCATGGGAG GACAAGCGTA AAGGTGACAG CCGGCCATCT 
CGACCACGAC TCAACTCCCA ACAGTCTATG GAGTATCTGA CGGAGGAGGA ATGTGCTCAG 
GTCCAGCAGC TTGGCTTAGT GGCCGACACG CTCATCAGCA GGATTCGGGA AGTGGCGAAG 
ACTCATAAGG TGGTGGAGCA GGAACTGGCT CATGCTGTAA ATGCCAGCGC TGCTGTCCTG 
AGTGAAGCAT TTCCTTCCAA ACCCTCCAGT CCTGAGGGTT TGAGTCGCAG TACAGCTGTA 
GATATTGTCA ACAGCAGTAC AGTTCTCCAG CAAGAGACCA AGATGCTCCT GGAGGGGAAA 
CTGCTGCAGC TGGAGTCTTC TCAGAAAGAG GAGCTTCTGT CCACAGTGGA GAATCTCAGC 
GCTGAATTAC AGAAGCTGGA GGACATTCAT TGGATCTGTC CCAGCATGCA CTGCTCTGAG 
GAGGAATCAT CCCGTGTGTC TGGAAAAAGC AGCATGAAGC TGAAAATCAT GCCCAAGGGG 
AAGAAAGAGA AGGAAAGGTT ACACAAGCAG CGCTCCAGCA GCTCTCTGTC AGGATCATGG 
GATATCAAAG CCAGCGTATG TGATGTGGAT CCGGCAGGAA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:dkey-172j4.3 ( XM_005172316.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172373.1 Danio rerio si:dkey-172j4.3 (si:dkey-172j4.3), transcript variant X2, mRNA.

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CloneID	ODa12940
Clone ID Related Accession (Same CDS sequence)	XM_005172317.4 , XM_005172316.3 , XM_021477787.1 , XM_005172317.3 , XM_005172316.4
Accession Version	XM_005172316.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	diacylglycerol kinase delta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005172316.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAA AGTTCAATCC AAACGTCCTT TATGTCCGGG AGCCCCAGGA GTCCCTGACC 
TCTCCAGACC CTCAAGTTGA GGCGGGTGAC GAATCATCAG ACAGTGACGG GGAGCCTGAA 
GAGACCTCAC ACAAACTCAT CCGAAAAGTG TCCACCTCTG GACAGATTCG AGCCAAGAAG 
AGTGTGAAGG AGGGTCTTCT GCTCAAACAG ACCAGCTCTT TTCAGAGGTG GAAGAGGAGG 
TATTTCAAAC TGCGTGGAAG GACACTTTAT TATGCCAAAG ATTCAAAGTC TCTCATATTT 
GATGAGGTGG ACCTGTCAGA TGCCAGTGTG GCCGAGACCA GCACCAAGAA CATCAACAAC 
AGTTTTACAG TCATCACTCC ATTCCGTAAG CTGATGTTGT GTGCGGAGAG CAGGAAGGAG 
ATGGAAGACT GGATCAGTGC TTTGAAGTCT GTACAGAAAT GGGAAACATA CGAGGCCAGT 
CAGTTCAACA TGGAGCACTT CTCCGGGATG CATAACTGGT ACGCCTGCTC TCATGCCCGG 
CCCACTTTCT GCAACGTGTG TCGAGAGGCT CTGCCGGGTG TTACCTCTCA TGGTCTCTCC 
TGTGAAGTGT GTAAGTTCAA AGCCCACAAG CGCTGTGCCG TACGATCCAC CAATACCTGT 
AAATGGACAA CTCTGGCATC TATAGGAAAT GACATCATCG AGGATGAGGA TGGAGTGTCA 
ATGCCTCACC AGTGGTTGGA GGGAAACCTG CCGGTCAGCG CTAAGTGTGT GGTGTGCGAT 
CGAAACTGCG GCAGCGTCCG GCGACTACAG GACTGGCGGT GTCTCTGGTG CAAAGCCATC 
GTGCACAACA GCTGTAAAGA GCAGTTGGGG AAGGTTTGTC CTCTGGGTCA GTGTAAGGTC 
TCCATCATTC CACCCACTGC ACTTAACAGC ATCGACTCTG ACGGCTTCTG GAAGGCCACC 
AGTCCATCCT GCTCCAGTCC TCTTCTGGTG CTGGTGAACA GTAAGAGTGG AGACAACCAG 
GGTGTCAAGT TCCTGCGCAG GTTTAAGCAG CTCCTGAATC CAGCGCAGGT CTTTGACCTC 
ATGAATGGCG GCCCACAGCT GGGGCTTCGT CTCTTCCAGA AGTTTGAGAC ATTTCGTACC 
CTGGTGTGCG GTGGAGATGG GAGCGTGGGC TGGGTTCTTT CTGAACTGGA CAAACTCAGC 
CTGCACAAAC AGTGTCAGCT GGGTGTGCTG CCGCTGGGCA CTGGAAATGA CCTGGCGCGT 
GTATTGGGCT GGGGAGGCCT GTGTGACGAC GACGCTCAGC TGCTGCAGAT ACTGGAGAAA 
CTGGAGAGAG CAACAACCAA GATGCTGGAC AGATGGAGTG TGATGACCTA TGAAGTGCCC 
ACCAAACAGA CTCCAGCAAT CGTAAAAGAC GACGACCCAT CAGATTCCCC TCTACAGATT 
ACACAATATG CTGATTCTGT TGCCACTCAC TTGACCAAAA TCCTGGACTC TGACAAACAC 
AGTGACGTTA TTTCATCTGC CAAGTTTCTA TGTGGAACAG TTAATGATTT TGTGGCTGAG 
GTCGGCAAAG CTTATGAACG GGCAACAGAG AACAAGGAGG AGGCCGATGC CATGGCTAAA 
AAGTGTGCCA TGCTGAATGA GAAGCTGGAC TCGTTGGTTA AGGCTCTCAG TGAAGATGCC 
AATGCCCAGC TGGTGCCAGC CGGGTCCATT TCTCCAGACG ACAGCGACAC GGATCAGTCC 
AGACCCTTCA GATCTAGAGA GCAGCTAATG CTGAGGGCCA ACAGCCTGAA GAAAGCACTC 
CGACAAATCA TAGATCAAGC AGAAAAAGTG GTGGATGAGC AGAACAGACA CACACAGGTG 
CAGAGAATGT CTTCCACCTC GTCAATCAAA AGAGGCAGCA GTGAGGACCT CAAGGAGGCG 
GAAACACGTC AAAATACATT GAGTCCGACC GCTACAACTC CTATATTACT GGAGAAGTCA 
GAAAGCTTCG GCACCGTGCT CTTCTCTGAA GAATCTGTGC AGTGCACAGA GAAGTGTGTG 
ATGAATAATT ATTTTGGCAT TGGGCTAGAT GCCAAAATAT CTCTAGAGTT CAACAACAAG 
AGAGACGAGA ACCCCAAAAA ATGCAGTAGT CGCACTAAAA ACATGATGTG GTACGGAGTG 
CTAGGGACCA AAGAACTGGT GCAGAAAACC TACAAGAACC TGGAACAAAG AGTCCAGCTG 
GAGTGTGATG GCGTGTCCAT GTCCTTGCCG AGTCTTCAGG GTCTGGCTGT TTTGAACATC 
CCCAGCTATG CAGGAGGCAT CAATTTCTGG GGAGGCACCA AAGAGGACAA TAATTTTGGA 
GCTCCATCTT TTGATGATAA GAAGCTGGAG GTGGTGGCTG TGTTTGGCAG CATGCAGATG 
GCAATGTCTC GGGTCATCAA CATACAGCAT CACCGCATCG CACAGTGTCG GCAGGTAAAG 
ATCACCATCC TTGGTGATGA AGGTGTTCCT GTGCAGGTGG ATGGTGAGGC CTGGATTCAG 
CCTCCGGGCA TCGTCCAAAT TGTTCACAAG AACCGAGCCC AGATGCTCAC CAGAGACCGG 
GCCTTCGAGA GCACGCTGAA ATCATGGGAG GACAAGCGTA AAGGTGACAG CCGGCCATCT 
CGACCACGAC TCAACTCCCA ACAGTCTATG GAGTATCTGA CGGAGGAGGA ATGTGCTCAG 
GTCCAGCAGC TTGGCTTAGT GGCCGACACG CTCATCAGCA GGATTCGGGA AGTGGCGAAG 
ACTCATAAGG TGGTGGAGCA GGAACTGGCT CATGCTGTAA ATGCCAGCGC TGCTGTCCTG 
AGTGAAGCAT TTCCTTCCAA ACCCTCCAGT CCTGAGGGTT TGAGTCGCAG TACAGCTGTA 
GATATTGTCA ACAGCAGTAC AGTTCTCCAG CAAGAGACCA AGATGCTCCT GGAGGGGAAA 
CTGCTGCAGC TGGAGTCTTC TCAGAAAGAG GAGCTTCTGT CCACAGTGGA GAATCTCAGC 
GCTGAATTAC AGAAGCTGGA GGACATTCAT TGGATCTGTC CCAGCATGCA CTGCTCTGAG 
GAGGAATCAT CCCGTGTGTC TGGAAAAAGC AGCATGAAGC TGAAAATCAT GCCCAAGGGG 
AAGAAAGAGA AGGAAAGGTT ACACAAGCAG CGCTCCAGCA GCTCTCTGTC AGGATCATGG 
GATATCAAAG CCAGCGTATG TGATGTGGAT CCGGCAGGAA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172373.1
CDS	234..3458
Translation	MASKFNPNVLYVREPQESLTSPDPQVEAGDESSDSDGEPEETSHKLIRKVSTSGQIRAKKSVKEGLLLKQTSSFQRWKRRYFKLRGRTLYYAKDSKSLIFDEVDLSDASVAETSTKNINNSFTVITPFRKLMLCAESRKEMEDWISALKSVQKWETYEASQFNMEHFSGMHNWYACSHARPTFCNVCREALPGVTSHGLSCEVCKFKAHKRCAVRSTNTCKWTTLASIGNDIIEDEDGVSMPHQWLEGNLPVSAKCVVCDRNCGSVRRLQDWRCLWCKAIVHNSCKEQLGKVCPLGQCKVSIIPPTALNSIDSDGFWKATSPSCSSPLLVLVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPQLGLRLFQKFETFRTLVCGGDGSVGWVLSELDKLSLHKQCQLGVLPLGTGNDLARVLGWGGLCDDDAQLLQILEKLERATTKMLDRWSVMTYEVPTKQTPAIVKDDDPSDSPLQITQYADSVATHLTKILDSDKHSDVISSAKFLCGTVNDFVAEVGKAYERATENKEEADAMAKKCAMLNEKLDSLVKALSEDANAQLVPAGSISPDDSDTDQSRPFRSREQLMLRANSLKKALRQIIDQAEKVVDEQNRHTQVQRMSSTSSIKRGSSEDLKEAETRQNTLSPTATTPILLEKSESFGTVLFSEESVQCTEKCVMNNYFGIGLDAKISLEFNNKRDENPKKCSSRTKNMMWYGVLGTKELVQKTYKNLEQRVQLECDGVSMSLPSLQGLAVLNIPSYAGGINFWGGTKEDNNFGAPSFDDKKLEVVAVFGSMQMAMSRVINIQHHRIAQCRQVKITILGDEGVPVQVDGEAWIQPPGIVQIVHKNRAQMLTRDRAFESTLKSWEDKRKGDSRPSRPRLNSQQSMEYLTEEECAQVQQLGLVADTLISRIREVAKTHKVVEQELAHAVNASAAVLSEAFPSKPSSPEGLSRSTAVDIVNSSTVLQQETKMLLEGKLLQLESSQKEELLSTVENLSAELQKLEDIHWICPSMHCSEEESSRVSGKSSMKLKIMPKGKKEKERLHKQRSSSSLSGSWDIKASVCDVDPAGN

Target ORF information:

RefSeq Version	XM_005172316.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:dkey-172j4.3 (si:dkey-172j4.3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172316.4

ORF Insert Sequence:

ATGGCCTCAA AGTTCAATCC AAACGTCCTT TATGTCCGGG AGCCCCAGGA GTCCCTGACC 
TCTCCAGACC CTCAAGTTGA GGCGGGTGAC GAATCATCAG ACAGTGACGG GGAGCCTGAA 
GAGACCTCAC ACAAACTCAT CCGAAAAGTG TCCACCTCTG GACAGATTCG AGCCAAGAAG 
AGTGTGAAGG AGGGTCTTCT GCTCAAACAG ACCAGCTCTT TTCAGAGGTG GAAGAGGAGG 
TATTTCAAAC TGCGTGGAAG GACACTTTAT TATGCCAAAG ATTCAAAGTC TCTCATATTT 
GATGAGGTGG ACCTGTCAGA TGCCAGTGTG GCCGAGACCA GCACCAAGAA CATCAACAAC 
AGTTTTACAG TCATCACTCC ATTCCGTAAG CTGATGTTGT GTGCGGAGAG CAGGAAGGAG 
ATGGAAGACT GGATCAGTGC TTTGAAGTCT GTACAGAAAT GGGAAACATA CGAGGCCAGT 
CAGTTCAACA TGGAGCACTT CTCCGGGATG CATAACTGGT ACGCCTGCTC TCATGCCCGG 
CCCACTTTCT GCAACGTGTG TCGAGAGGCT CTGCCGGGTG TTACCTCTCA TGGTCTCTCC 
TGTGAAGTGT GTAAGTTCAA AGCCCACAAG CGCTGTGCCG TACGATCCAC CAATACCTGT 
AAATGGACAA CTCTGGCATC TATAGGAAAT GACATCATCG AGGATGAGGA TGGAGTGTCA 
ATGCCTCACC AGTGGTTGGA GGGAAACCTG CCGGTCAGCG CTAAGTGTGT GGTGTGCGAT 
CGAAACTGCG GCAGCGTCCG GCGACTACAG GACTGGCGGT GTCTCTGGTG CAAAGCCATC 
GTGCACAACA GCTGTAAAGA GCAGTTGGGG AAGGTTTGTC CTCTGGGTCA GTGTAAGGTC 
TCCATCATTC CACCCACTGC ACTTAACAGC ATCGACTCTG ACGGCTTCTG GAAGGCCACC 
AGTCCATCCT GCTCCAGTCC TCTTCTGGTG CTGGTGAACA GTAAGAGTGG AGACAACCAG 
GGTGTCAAGT TCCTGCGCAG GTTTAAGCAG CTCCTGAATC CAGCGCAGGT CTTTGACCTC 
ATGAATGGCG GCCCACAGCT GGGGCTTCGT CTCTTCCAGA AGTTTGAGAC ATTTCGTACC 
CTGGTGTGCG GTGGAGATGG GAGCGTGGGC TGGGTTCTTT CTGAACTGGA CAAACTCAGC 
CTGCACAAAC AGTGTCAGCT GGGTGTGCTG CCGCTGGGCA CTGGAAATGA CCTGGCGCGT 
GTATTGGGCT GGGGAGGCCT GTGTGACGAC GACGCTCAGC TGCTGCAGAT ACTGGAGAAA 
CTGGAGAGAG CAACAACCAA GATGCTGGAC AGATGGAGTG TGATGACCTA TGAAGTGCCC 
ACCAAACAGA CTCCAGCAAT CGTAAAAGAC GACGACCCAT CAGATTCCCC TCTACAGATT 
ACACAATATG CTGATTCTGT TGCCACTCAC TTGACCAAAA TCCTGGACTC TGACAAACAC 
AGTGACGTTA TTTCATCTGC CAAGTTTCTA TGTGGAACAG TTAATGATTT TGTGGCTGAG 
GTCGGCAAAG CTTATGAACG GGCAACAGAG AACAAGGAGG AGGCCGATGC CATGGCTAAA 
AAGTGTGCCA TGCTGAATGA GAAGCTGGAC TCGTTGGTTA AGGCTCTCAG TGAAGATGCC 
AATGCCCAGC TGGTGCCAGC CGGGTCCATT TCTCCAGACG ACAGCGACAC GGATCAGTCC 
AGACCCTTCA GATCTAGAGA GCAGCTAATG CTGAGGGCCA ACAGCCTGAA GAAAGCACTC 
CGACAAATCA TAGATCAAGC AGAAAAAGTG GTGGATGAGC AGAACAGACA CACACAGGTG 
CAGAGAATGT CTTCCACCTC GTCAATCAAA AGAGGCAGCA GTGAGGACCT CAAGGAGGCG 
GAAACACGTC AAAATACATT GAGTCCGACC GCTACAACTC CTATATTACT GGAGAAGTCA 
GAAAGCTTCG GCACCGTGCT CTTCTCTGAA GAATCTGTGC AGTGCACAGA GAAGTGTGTG 
ATGAATAATT ATTTTGGCAT TGGGCTAGAT GCCAAAATAT CTCTAGAGTT CAACAACAAG 
AGAGACGAGA ACCCCAAAAA ATGCAGTAGT CGCACTAAAA ACATGATGTG GTACGGAGTG 
CTAGGGACCA AAGAACTGGT GCAGAAAACC TACAAGAACC TGGAACAAAG AGTCCAGCTG 
GAGTGTGATG GCGTGTCCAT GTCCTTGCCG AGTCTTCAGG GTCTGGCTGT TTTGAACATC 
CCCAGCTATG CAGGAGGCAT CAATTTCTGG GGAGGCACCA AAGAGGACAA TAATTTTGGA 
GCTCCATCTT TTGATGATAA GAAGCTGGAG GTGGTGGCTG TGTTTGGCAG CATGCAGATG 
GCAATGTCTC GGGTCATCAA CATACAGCAT CACCGCATCG CACAGTGTCG GCAGGTAAAG 
ATCACCATCC TTGGTGATGA AGGTGTTCCT GTGCAGGTGG ATGGTGAGGC CTGGATTCAG 
CCTCCGGGCA TCGTCCAAAT TGTTCACAAG AACCGAGCCC AGATGCTCAC CAGAGACCGG 
GCCTTCGAGA GCACGCTGAA ATCATGGGAG GACAAGCGTA AAGGTGACAG CCGGCCATCT 
CGACCACGAC TCAACTCCCA ACAGTCTATG GAGTATCTGA CGGAGGAGGA ATGTGCTCAG 
GTCCAGCAGC TTGGCTTAGT GGCCGACACG CTCATCAGCA GGATTCGGGA AGTGGCGAAG 
ACTCATAAGG TGGTGGAGCA GGAACTGGCT CATGCTGTAA ATGCCAGCGC TGCTGTCCTG 
AGTGAAGCAT TTCCTTCCAA ACCCTCCAGT CCTGAGGGTT TGAGTCGCAG TACAGCTGTA 
GATATTGTCA ACAGCAGTAC AGTTCTCCAG CAAGAGACCA AGATGCTCCT GGAGGGGAAA 
CTGCTGCAGC TGGAGTCTTC TCAGAAAGAG GAGCTTCTGT CCACAGTGGA GAATCTCAGC 
GCTGAATTAC AGAAGCTGGA GGACATTCAT TGGATCTGTC CCAGCATGCA CTGCTCTGAG 
GAGGAATCAT CCCGTGTGTC TGGAAAAAGC AGCATGAAGC TGAAAATCAT GCCCAAGGGG 
AAGAAAGAGA AGGAAAGGTT ACACAAGCAG CGCTCCAGCA GCTCTCTGTC AGGATCATGG 
GATATCAAAG CCAGCGTATG TGATGTGGAT CCGGCAGGAA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:dkey-172j4.3 ( XM_005172318.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172375.1 Danio rerio si:dkey-172j4.3 (si:dkey-172j4.3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa12941
Clone ID Related Accession (Same CDS sequence)	XM_005172318.3 , XM_005172318.4
Accession Version	XM_005172318.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	diacylglycerol kinase kappa isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172318.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAGAC TGTGTCCAGG CAGTGTGTGT TGCTTTGCCC CTCTTTTGTT CGGCTGTGAT 
GGAGGTGTCC AGGGAGATGC CAGTCGATTG AGAGAGGAAG ACTTTTTGGA TACCTCTTCC 
AGCAGTTCAG TGAGAACAAC AGGAGGAAAG GCAGTCAGCA ATAGCCTCTT CTCACAGAAG 
AGTGTGAAGG AGGGTCTTCT GCTCAAACAG ACCAGCTCTT TTCAGAGGTG GAAGAGGAGG 
TATTTCAAAC TGCGTGGAAG GACACTTTAT TATGCCAAAG ATTCAAAGTC TCTCATATTT 
GATGAGGTGG ACCTGTCAGA TGCCAGTGTG GCCGAGACCA GCACCAAGAA CATCAACAAC 
AGTTTTACAG TCATCACTCC ATTCCGTAAG CTGATGTTGT GTGCGGAGAG CAGGAAGGAG 
ATGGAAGACT GGATCAGTGC TTTGAAGTCT GTACAGAAAT GGGAAACATA CGAGGCCAGT 
CAGTTCAACA TGGAGCACTT CTCCGGGATG CATAACTGGT ACGCCTGCTC TCATGCCCGG 
CCCACTTTCT GCAACGTGTG TCGAGAGGCT CTGCCGGGTG TTACCTCTCA TGGTCTCTCC 
TGTGAAGTGT GTAAGTTCAA AGCCCACAAG CGCTGTGCCG TACGATCCAC CAATACCTGT 
AAATGGACAA CTCTGGCATC TATAGGAAAT GACATCATCG AGGATGAGGA TGGAGTGTCA 
ATGCCTCACC AGTGGTTGGA GGGAAACCTG CCGGTCAGCG CTAAGTGTGT GGTGTGCGAT 
CGAAACTGCG GCAGCGTCCG GCGACTACAG GACTGGCGGT GTCTCTGGTG CAAAGCCATC 
GTGCACAACA GCTGTAAAGA GCAGTTGGGG AAGGTTTGTC CTCTGGGTCA GTGTAAGGTC 
TCCATCATTC CACCCACTGC ACTTAACAGC ATCGACTCTG ACGGCTTCTG GAAGGCCACC 
AGTCCATCCT GCTCCAGTCC TCTTCTGGTG CTGGTGAACA GTAAGAGTGG AGACAACCAG 
GGTGTCAAGT TCCTGCGCAG GTTTAAGCAG CTCCTGAATC CAGCGCAGGT CTTTGACCTC 
ATGAATGGCG GCCCACAGCT GGGGCTTCGT CTCTTCCAGA AGTTTGAGAC ATTTCGTACC 
CTGGTGTGCG GTGGAGATGG GAGCGTGGGC TGGGTTCTTT CTGAACTGGA CAAACTCAGC 
CTGCACAAAC AGTGTCAGCT GGGTGTGCTG CCGCTGGGCA CTGGAAATGA CCTGGCGCGT 
GTATTGGGCT GGGGAGGCCT GTGTGACGAC GACGCTCAGC TGCTGCAGAT ACTGGAGAAA 
CTGGAGAGAG CAACAACCAA GATGCTGGAC AGATGGAGTG TGATGACCTA TGAAGTGCCC 
ACCAAACAGA CTCCAGCAAT CGTAAAAGAC GACGACCCAT CAGATTCCCC TCTACAGATT 
ACACAATATG CTGATTCTGT TGCCACTCAC TTGACCAAAA TCCTGGACTC TGACAAACAC 
AGTGACGTTA TTTCATCTGC CAAGTTTCTA TGTGGAACAG TTAATGATTT TGTGGCTGAG 
GTCGGCAAAG CTTATGAACG GGCAACAGAG AACAAGGAGG AGGCCGATGC CATGGCTAAA 
AAGTGTGCCA TGCTGAATGA GAAGCTGGAC TCGTTGGTTA AGGCTCTCAG TGAAGATGCC 
AATGCCCAGC TGGTGCCAGC CGGGTCCATT TCTCCAGACG ACAGCGACAC GGATCAGTCC 
AGACCCTTCA GATCTAGAGA GCAGCTAATG CTGAGGGCCA ACAGCCTGAA GAAAGCACTC 
CGACAAATCA TAGATCAAGC AGAAAAAGTG GTGGATGAGC AGAACAGACA CACACAGGTG 
CAGAGAATGT CTTCCACCTC GTCAATCAAA AGAGGCAGCA GTGAGGACCT CAAGGAGGCG 
GAAACACGTC AAAATACATT GAGTCCGACC GCTACAACTC CTATATTACT GGAGAAGTCA 
GAAAGCTTCG GCACCGTGCT CTTCTCTGAA GAATCTGTGC AGTGCACAGA GAAGTGTGTG 
ATGAATAATT ATTTTGGCAT TGGGCTAGAT GCCAAAATAT CTCTAGAGTT CAACAACAAG 
AGAGACGAGA ACCCCAAAAA ATGCAGTAGT CGCACTAAAA ACATGATGTG GTACGGAGTG 
CTAGGGACCA AAGAACTGGT GCAGAAAACC TACAAGAACC TGGAACAAAG AGTCCAGCTG 
GAGTGTGATG GCGTGTCCAT GTCCTTGCCG AGTCTTCAGG GTCTGGCTGT TTTGAACATC 
CCCAGCTATG CAGGAGGCAT CAATTTCTGG GGAGGCACCA AAGAGGACAA TAATTTTGGA 
GCTCCATCTT TTGATGATAA GAAGCTGGAG GTGGTGGCTG TGTTTGGCAG CATGCAGATG 
GCAATGTCTC GGGTCATCAA CATACAGCAT CACCGCATCG CACAGTGTCG GCAGGTAAAG 
ATCACCATCC TTGGTGATGA AGGTGTTCCT GTGCAGGTGG ATGGTGAGGC CTGGATTCAG 
CCTCCGGGCA TCGTCCAAAT TGTTCACAAG AACCGAGCCC AGATGCTCAC CAGAGACCGG 
GCCTTCGAGA GCACGCTGAA ATCATGGGAG GACAAGCGTA AAGGTGACAG CCGGCCATCT 
CGACCACGAC TCAACTCCCA ACAGTCTATG GAGTATCTGA CGGAGGAGGA ATGTGCTCAG 
GTCCAGCAGC TTGGCTTAGT GGCCGACACG CTCATCAGCA GGATTCGGGA AGTGGCGAAG 
ACTCATAAGG TGGTGGAGCA GGAACTGGCT CATGCTGTAA ATGCCAGCGC TGCTGTCCTG 
AGTGAAGCAT TTCCTTCCAA ACCCTCCAGT CCTGAGGGTT TGAGTCGCAG TACAGCTGTA 
GATATTGTCA ACAGCAGTAC AGTTCTCCAG CAAGAGACCA AGATGCTCCT GGAGGGGAAA 
CTGCTGCAGC TGGAGTCTTC TCAGAAAGAG GAGCTTCTGT CCACAGTGGA GAATCTCAGC 
GCTGAATTAC AGAAGCTGGA GGACATTCAT TGGATCTGTC CCAGCATGCA CTGCTCTGAG 
GAGGAATCAT CCCGTGTGTC TGGAAAAAGC AGCATGAAGC TGAAAATCAT GCCCAAGGGG 
AAGAAAGAGA AGGAAAGGTT ACACAAGCAG CGCTCCAGCA GCTCTCTGTC AGGATCATGG 
GATATCAAAG CCAGCGTATG TGATGTGGAT CCGGCAGGAA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172375.1
CDS	137..3361
Translation	MPRLCPGSVCCFAPLLFGCDGGVQGDASRLREEDFLDTSSSSSVRTTGGKAVSNSLFSQKSVKEGLLLKQTSSFQRWKRRYFKLRGRTLYYAKDSKSLIFDEVDLSDASVAETSTKNINNSFTVITPFRKLMLCAESRKEMEDWISALKSVQKWETYEASQFNMEHFSGMHNWYACSHARPTFCNVCREALPGVTSHGLSCEVCKFKAHKRCAVRSTNTCKWTTLASIGNDIIEDEDGVSMPHQWLEGNLPVSAKCVVCDRNCGSVRRLQDWRCLWCKAIVHNSCKEQLGKVCPLGQCKVSIIPPTALNSIDSDGFWKATSPSCSSPLLVLVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPQLGLRLFQKFETFRTLVCGGDGSVGWVLSELDKLSLHKQCQLGVLPLGTGNDLARVLGWGGLCDDDAQLLQILEKLERATTKMLDRWSVMTYEVPTKQTPAIVKDDDPSDSPLQITQYADSVATHLTKILDSDKHSDVISSAKFLCGTVNDFVAEVGKAYERATENKEEADAMAKKCAMLNEKLDSLVKALSEDANAQLVPAGSISPDDSDTDQSRPFRSREQLMLRANSLKKALRQIIDQAEKVVDEQNRHTQVQRMSSTSSIKRGSSEDLKEAETRQNTLSPTATTPILLEKSESFGTVLFSEESVQCTEKCVMNNYFGIGLDAKISLEFNNKRDENPKKCSSRTKNMMWYGVLGTKELVQKTYKNLEQRVQLECDGVSMSLPSLQGLAVLNIPSYAGGINFWGGTKEDNNFGAPSFDDKKLEVVAVFGSMQMAMSRVINIQHHRIAQCRQVKITILGDEGVPVQVDGEAWIQPPGIVQIVHKNRAQMLTRDRAFESTLKSWEDKRKGDSRPSRPRLNSQQSMEYLTEEECAQVQQLGLVADTLISRIREVAKTHKVVEQELAHAVNASAAVLSEAFPSKPSSPEGLSRSTAVDIVNSSTVLQQETKMLLEGKLLQLESSQKEELLSTVENLSAELQKLEDIHWICPSMHCSEEESSRVSGKSSMKLKIMPKGKKEKERLHKQRSSSSLSGSWDIKASVCDVDPAGN

Target ORF information:

RefSeq Version	XM_005172318.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:dkey-172j4.3 (si:dkey-172j4.3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172318.3

ORF Insert Sequence:

ATGCCCAGAC TGTGTCCAGG CAGTGTGTGT TGCTTTGCCC CTCTTTTGTT CGGCTGTGAT 
GGAGGTGTCC AGGGAGATGC CAGTCGATTG AGAGAGGAAG ACTTTTTGGA TACCTCTTCC 
AGCAGTTCAG TGAGAACAAC AGGAGGAAAG GCAGTCAGCA ATAGCCTCTT CTCACAGAAG 
AGTGTGAAGG AGGGTCTTCT GCTCAAACAG ACCAGCTCTT TTCAGAGGTG GAAGAGGAGG 
TATTTCAAAC TGCGTGGAAG GACACTTTAT TATGCCAAAG ATTCAAAGTC TCTCATATTT 
GATGAGGTGG ACCTGTCAGA TGCCAGTGTG GCCGAGACCA GCACCAAGAA CATCAACAAC 
AGTTTTACAG TCATCACTCC ATTCCGTAAG CTGATGTTGT GTGCGGAGAG CAGGAAGGAG 
ATGGAAGACT GGATCAGTGC TTTGAAGTCT GTACAGAAAT GGGAAACATA CGAGGCCAGT 
CAGTTCAACA TGGAGCACTT CTCCGGGATG CATAACTGGT ACGCCTGCTC TCATGCCCGG 
CCCACTTTCT GCAACGTGTG TCGAGAGGCT CTGCCGGGTG TTACCTCTCA TGGTCTCTCC 
TGTGAAGTGT GTAAGTTCAA AGCCCACAAG CGCTGTGCCG TACGATCCAC CAATACCTGT 
AAATGGACAA CTCTGGCATC TATAGGAAAT GACATCATCG AGGATGAGGA TGGAGTGTCA 
ATGCCTCACC AGTGGTTGGA GGGAAACCTG CCGGTCAGCG CTAAGTGTGT GGTGTGCGAT 
CGAAACTGCG GCAGCGTCCG GCGACTACAG GACTGGCGGT GTCTCTGGTG CAAAGCCATC 
GTGCACAACA GCTGTAAAGA GCAGTTGGGG AAGGTTTGTC CTCTGGGTCA GTGTAAGGTC 
TCCATCATTC CACCCACTGC ACTTAACAGC ATCGACTCTG ACGGCTTCTG GAAGGCCACC 
AGTCCATCCT GCTCCAGTCC TCTTCTGGTG CTGGTGAACA GTAAGAGTGG AGACAACCAG 
GGTGTCAAGT TCCTGCGCAG GTTTAAGCAG CTCCTGAATC CAGCGCAGGT CTTTGACCTC 
ATGAATGGCG GCCCACAGCT GGGGCTTCGT CTCTTCCAGA AGTTTGAGAC ATTTCGTACC 
CTGGTGTGCG GTGGAGATGG GAGCGTGGGC TGGGTTCTTT CTGAACTGGA CAAACTCAGC 
CTGCACAAAC AGTGTCAGCT GGGTGTGCTG CCGCTGGGCA CTGGAAATGA CCTGGCGCGT 
GTATTGGGCT GGGGAGGCCT GTGTGACGAC GACGCTCAGC TGCTGCAGAT ACTGGAGAAA 
CTGGAGAGAG CAACAACCAA GATGCTGGAC AGATGGAGTG TGATGACCTA TGAAGTGCCC 
ACCAAACAGA CTCCAGCAAT CGTAAAAGAC GACGACCCAT CAGATTCCCC TCTACAGATT 
ACACAATATG CTGATTCTGT TGCCACTCAC TTGACCAAAA TCCTGGACTC TGACAAACAC 
AGTGACGTTA TTTCATCTGC CAAGTTTCTA TGTGGAACAG TTAATGATTT TGTGGCTGAG 
GTCGGCAAAG CTTATGAACG GGCAACAGAG AACAAGGAGG AGGCCGATGC CATGGCTAAA 
AAGTGTGCCA TGCTGAATGA GAAGCTGGAC TCGTTGGTTA AGGCTCTCAG TGAAGATGCC 
AATGCCCAGC TGGTGCCAGC CGGGTCCATT TCTCCAGACG ACAGCGACAC GGATCAGTCC 
AGACCCTTCA GATCTAGAGA GCAGCTAATG CTGAGGGCCA ACAGCCTGAA GAAAGCACTC 
CGACAAATCA TAGATCAAGC AGAAAAAGTG GTGGATGAGC AGAACAGACA CACACAGGTG 
CAGAGAATGT CTTCCACCTC GTCAATCAAA AGAGGCAGCA GTGAGGACCT CAAGGAGGCG 
GAAACACGTC AAAATACATT GAGTCCGACC GCTACAACTC CTATATTACT GGAGAAGTCA 
GAAAGCTTCG GCACCGTGCT CTTCTCTGAA GAATCTGTGC AGTGCACAGA GAAGTGTGTG 
ATGAATAATT ATTTTGGCAT TGGGCTAGAT GCCAAAATAT CTCTAGAGTT CAACAACAAG 
AGAGACGAGA ACCCCAAAAA ATGCAGTAGT CGCACTAAAA ACATGATGTG GTACGGAGTG 
CTAGGGACCA AAGAACTGGT GCAGAAAACC TACAAGAACC TGGAACAAAG AGTCCAGCTG 
GAGTGTGATG GCGTGTCCAT GTCCTTGCCG AGTCTTCAGG GTCTGGCTGT TTTGAACATC 
CCCAGCTATG CAGGAGGCAT CAATTTCTGG GGAGGCACCA AAGAGGACAA TAATTTTGGA 
GCTCCATCTT TTGATGATAA GAAGCTGGAG GTGGTGGCTG TGTTTGGCAG CATGCAGATG 
GCAATGTCTC GGGTCATCAA CATACAGCAT CACCGCATCG CACAGTGTCG GCAGGTAAAG 
ATCACCATCC TTGGTGATGA AGGTGTTCCT GTGCAGGTGG ATGGTGAGGC CTGGATTCAG 
CCTCCGGGCA TCGTCCAAAT TGTTCACAAG AACCGAGCCC AGATGCTCAC CAGAGACCGG 
GCCTTCGAGA GCACGCTGAA ATCATGGGAG GACAAGCGTA AAGGTGACAG CCGGCCATCT 
CGACCACGAC TCAACTCCCA ACAGTCTATG GAGTATCTGA CGGAGGAGGA ATGTGCTCAG 
GTCCAGCAGC TTGGCTTAGT GGCCGACACG CTCATCAGCA GGATTCGGGA AGTGGCGAAG 
ACTCATAAGG TGGTGGAGCA GGAACTGGCT CATGCTGTAA ATGCCAGCGC TGCTGTCCTG 
AGTGAAGCAT TTCCTTCCAA ACCCTCCAGT CCTGAGGGTT TGAGTCGCAG TACAGCTGTA 
GATATTGTCA ACAGCAGTAC AGTTCTCCAG CAAGAGACCA AGATGCTCCT GGAGGGGAAA 
CTGCTGCAGC TGGAGTCTTC TCAGAAAGAG GAGCTTCTGT CCACAGTGGA GAATCTCAGC 
GCTGAATTAC AGAAGCTGGA GGACATTCAT TGGATCTGTC CCAGCATGCA CTGCTCTGAG 
GAGGAATCAT CCCGTGTGTC TGGAAAAAGC AGCATGAAGC TGAAAATCAT GCCCAAGGGG 
AAGAAAGAGA AGGAAAGGTT ACACAAGCAG CGCTCCAGCA GCTCTCTGTC AGGATCATGG 
GATATCAAAG CCAGCGTATG TGATGTGGAT CCGGCAGGAA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:dkey-172j4.3 ( XM_005172318.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172375.1 Danio rerio si:dkey-172j4.3 (si:dkey-172j4.3), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa12941
Clone ID Related Accession (Same CDS sequence)	XM_005172318.3 , XM_005172318.4
Accession Version	XM_005172318.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	diacylglycerol kinase delta isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005172318.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAGAC TGTGTCCAGG CAGTGTGTGT TGCTTTGCCC CTCTTTTGTT CGGCTGTGAT 
GGAGGTGTCC AGGGAGATGC CAGTCGATTG AGAGAGGAAG ACTTTTTGGA TACCTCTTCC 
AGCAGTTCAG TGAGAACAAC AGGAGGAAAG GCAGTCAGCA ATAGCCTCTT CTCACAGAAG 
AGTGTGAAGG AGGGTCTTCT GCTCAAACAG ACCAGCTCTT TTCAGAGGTG GAAGAGGAGG 
TATTTCAAAC TGCGTGGAAG GACACTTTAT TATGCCAAAG ATTCAAAGTC TCTCATATTT 
GATGAGGTGG ACCTGTCAGA TGCCAGTGTG GCCGAGACCA GCACCAAGAA CATCAACAAC 
AGTTTTACAG TCATCACTCC ATTCCGTAAG CTGATGTTGT GTGCGGAGAG CAGGAAGGAG 
ATGGAAGACT GGATCAGTGC TTTGAAGTCT GTACAGAAAT GGGAAACATA CGAGGCCAGT 
CAGTTCAACA TGGAGCACTT CTCCGGGATG CATAACTGGT ACGCCTGCTC TCATGCCCGG 
CCCACTTTCT GCAACGTGTG TCGAGAGGCT CTGCCGGGTG TTACCTCTCA TGGTCTCTCC 
TGTGAAGTGT GTAAGTTCAA AGCCCACAAG CGCTGTGCCG TACGATCCAC CAATACCTGT 
AAATGGACAA CTCTGGCATC TATAGGAAAT GACATCATCG AGGATGAGGA TGGAGTGTCA 
ATGCCTCACC AGTGGTTGGA GGGAAACCTG CCGGTCAGCG CTAAGTGTGT GGTGTGCGAT 
CGAAACTGCG GCAGCGTCCG GCGACTACAG GACTGGCGGT GTCTCTGGTG CAAAGCCATC 
GTGCACAACA GCTGTAAAGA GCAGTTGGGG AAGGTTTGTC CTCTGGGTCA GTGTAAGGTC 
TCCATCATTC CACCCACTGC ACTTAACAGC ATCGACTCTG ACGGCTTCTG GAAGGCCACC 
AGTCCATCCT GCTCCAGTCC TCTTCTGGTG CTGGTGAACA GTAAGAGTGG AGACAACCAG 
GGTGTCAAGT TCCTGCGCAG GTTTAAGCAG CTCCTGAATC CAGCGCAGGT CTTTGACCTC 
ATGAATGGCG GCCCACAGCT GGGGCTTCGT CTCTTCCAGA AGTTTGAGAC ATTTCGTACC 
CTGGTGTGCG GTGGAGATGG GAGCGTGGGC TGGGTTCTTT CTGAACTGGA CAAACTCAGC 
CTGCACAAAC AGTGTCAGCT GGGTGTGCTG CCGCTGGGCA CTGGAAATGA CCTGGCGCGT 
GTATTGGGCT GGGGAGGCCT GTGTGACGAC GACGCTCAGC TGCTGCAGAT ACTGGAGAAA 
CTGGAGAGAG CAACAACCAA GATGCTGGAC AGATGGAGTG TGATGACCTA TGAAGTGCCC 
ACCAAACAGA CTCCAGCAAT CGTAAAAGAC GACGACCCAT CAGATTCCCC TCTACAGATT 
ACACAATATG CTGATTCTGT TGCCACTCAC TTGACCAAAA TCCTGGACTC TGACAAACAC 
AGTGACGTTA TTTCATCTGC CAAGTTTCTA TGTGGAACAG TTAATGATTT TGTGGCTGAG 
GTCGGCAAAG CTTATGAACG GGCAACAGAG AACAAGGAGG AGGCCGATGC CATGGCTAAA 
AAGTGTGCCA TGCTGAATGA GAAGCTGGAC TCGTTGGTTA AGGCTCTCAG TGAAGATGCC 
AATGCCCAGC TGGTGCCAGC CGGGTCCATT TCTCCAGACG ACAGCGACAC GGATCAGTCC 
AGACCCTTCA GATCTAGAGA GCAGCTAATG CTGAGGGCCA ACAGCCTGAA GAAAGCACTC 
CGACAAATCA TAGATCAAGC AGAAAAAGTG GTGGATGAGC AGAACAGACA CACACAGGTG 
CAGAGAATGT CTTCCACCTC GTCAATCAAA AGAGGCAGCA GTGAGGACCT CAAGGAGGCG 
GAAACACGTC AAAATACATT GAGTCCGACC GCTACAACTC CTATATTACT GGAGAAGTCA 
GAAAGCTTCG GCACCGTGCT CTTCTCTGAA GAATCTGTGC AGTGCACAGA GAAGTGTGTG 
ATGAATAATT ATTTTGGCAT TGGGCTAGAT GCCAAAATAT CTCTAGAGTT CAACAACAAG 
AGAGACGAGA ACCCCAAAAA ATGCAGTAGT CGCACTAAAA ACATGATGTG GTACGGAGTG 
CTAGGGACCA AAGAACTGGT GCAGAAAACC TACAAGAACC TGGAACAAAG AGTCCAGCTG 
GAGTGTGATG GCGTGTCCAT GTCCTTGCCG AGTCTTCAGG GTCTGGCTGT TTTGAACATC 
CCCAGCTATG CAGGAGGCAT CAATTTCTGG GGAGGCACCA AAGAGGACAA TAATTTTGGA 
GCTCCATCTT TTGATGATAA GAAGCTGGAG GTGGTGGCTG TGTTTGGCAG CATGCAGATG 
GCAATGTCTC GGGTCATCAA CATACAGCAT CACCGCATCG CACAGTGTCG GCAGGTAAAG 
ATCACCATCC TTGGTGATGA AGGTGTTCCT GTGCAGGTGG ATGGTGAGGC CTGGATTCAG 
CCTCCGGGCA TCGTCCAAAT TGTTCACAAG AACCGAGCCC AGATGCTCAC CAGAGACCGG 
GCCTTCGAGA GCACGCTGAA ATCATGGGAG GACAAGCGTA AAGGTGACAG CCGGCCATCT 
CGACCACGAC TCAACTCCCA ACAGTCTATG GAGTATCTGA CGGAGGAGGA ATGTGCTCAG 
GTCCAGCAGC TTGGCTTAGT GGCCGACACG CTCATCAGCA GGATTCGGGA AGTGGCGAAG 
ACTCATAAGG TGGTGGAGCA GGAACTGGCT CATGCTGTAA ATGCCAGCGC TGCTGTCCTG 
AGTGAAGCAT TTCCTTCCAA ACCCTCCAGT CCTGAGGGTT TGAGTCGCAG TACAGCTGTA 
GATATTGTCA ACAGCAGTAC AGTTCTCCAG CAAGAGACCA AGATGCTCCT GGAGGGGAAA 
CTGCTGCAGC TGGAGTCTTC TCAGAAAGAG GAGCTTCTGT CCACAGTGGA GAATCTCAGC 
GCTGAATTAC AGAAGCTGGA GGACATTCAT TGGATCTGTC CCAGCATGCA CTGCTCTGAG 
GAGGAATCAT CCCGTGTGTC TGGAAAAAGC AGCATGAAGC TGAAAATCAT GCCCAAGGGG 
AAGAAAGAGA AGGAAAGGTT ACACAAGCAG CGCTCCAGCA GCTCTCTGTC AGGATCATGG 
GATATCAAAG CCAGCGTATG TGATGTGGAT CCGGCAGGAA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172375.1
CDS	93..3317
Translation	MPRLCPGSVCCFAPLLFGCDGGVQGDASRLREEDFLDTSSSSSVRTTGGKAVSNSLFSQKSVKEGLLLKQTSSFQRWKRRYFKLRGRTLYYAKDSKSLIFDEVDLSDASVAETSTKNINNSFTVITPFRKLMLCAESRKEMEDWISALKSVQKWETYEASQFNMEHFSGMHNWYACSHARPTFCNVCREALPGVTSHGLSCEVCKFKAHKRCAVRSTNTCKWTTLASIGNDIIEDEDGVSMPHQWLEGNLPVSAKCVVCDRNCGSVRRLQDWRCLWCKAIVHNSCKEQLGKVCPLGQCKVSIIPPTALNSIDSDGFWKATSPSCSSPLLVLVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPQLGLRLFQKFETFRTLVCGGDGSVGWVLSELDKLSLHKQCQLGVLPLGTGNDLARVLGWGGLCDDDAQLLQILEKLERATTKMLDRWSVMTYEVPTKQTPAIVKDDDPSDSPLQITQYADSVATHLTKILDSDKHSDVISSAKFLCGTVNDFVAEVGKAYERATENKEEADAMAKKCAMLNEKLDSLVKALSEDANAQLVPAGSISPDDSDTDQSRPFRSREQLMLRANSLKKALRQIIDQAEKVVDEQNRHTQVQRMSSTSSIKRGSSEDLKEAETRQNTLSPTATTPILLEKSESFGTVLFSEESVQCTEKCVMNNYFGIGLDAKISLEFNNKRDENPKKCSSRTKNMMWYGVLGTKELVQKTYKNLEQRVQLECDGVSMSLPSLQGLAVLNIPSYAGGINFWGGTKEDNNFGAPSFDDKKLEVVAVFGSMQMAMSRVINIQHHRIAQCRQVKITILGDEGVPVQVDGEAWIQPPGIVQIVHKNRAQMLTRDRAFESTLKSWEDKRKGDSRPSRPRLNSQQSMEYLTEEECAQVQQLGLVADTLISRIREVAKTHKVVEQELAHAVNASAAVLSEAFPSKPSSPEGLSRSTAVDIVNSSTVLQQETKMLLEGKLLQLESSQKEELLSTVENLSAELQKLEDIHWICPSMHCSEEESSRVSGKSSMKLKIMPKGKKEKERLHKQRSSSSLSGSWDIKASVCDVDPAGN

Target ORF information:

RefSeq Version	XM_005172318.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:dkey-172j4.3 (si:dkey-172j4.3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172318.4

ORF Insert Sequence:

ATGCCCAGAC TGTGTCCAGG CAGTGTGTGT TGCTTTGCCC CTCTTTTGTT CGGCTGTGAT 
GGAGGTGTCC AGGGAGATGC CAGTCGATTG AGAGAGGAAG ACTTTTTGGA TACCTCTTCC 
AGCAGTTCAG TGAGAACAAC AGGAGGAAAG GCAGTCAGCA ATAGCCTCTT CTCACAGAAG 
AGTGTGAAGG AGGGTCTTCT GCTCAAACAG ACCAGCTCTT TTCAGAGGTG GAAGAGGAGG 
TATTTCAAAC TGCGTGGAAG GACACTTTAT TATGCCAAAG ATTCAAAGTC TCTCATATTT 
GATGAGGTGG ACCTGTCAGA TGCCAGTGTG GCCGAGACCA GCACCAAGAA CATCAACAAC 
AGTTTTACAG TCATCACTCC ATTCCGTAAG CTGATGTTGT GTGCGGAGAG CAGGAAGGAG 
ATGGAAGACT GGATCAGTGC TTTGAAGTCT GTACAGAAAT GGGAAACATA CGAGGCCAGT 
CAGTTCAACA TGGAGCACTT CTCCGGGATG CATAACTGGT ACGCCTGCTC TCATGCCCGG 
CCCACTTTCT GCAACGTGTG TCGAGAGGCT CTGCCGGGTG TTACCTCTCA TGGTCTCTCC 
TGTGAAGTGT GTAAGTTCAA AGCCCACAAG CGCTGTGCCG TACGATCCAC CAATACCTGT 
AAATGGACAA CTCTGGCATC TATAGGAAAT GACATCATCG AGGATGAGGA TGGAGTGTCA 
ATGCCTCACC AGTGGTTGGA GGGAAACCTG CCGGTCAGCG CTAAGTGTGT GGTGTGCGAT 
CGAAACTGCG GCAGCGTCCG GCGACTACAG GACTGGCGGT GTCTCTGGTG CAAAGCCATC 
GTGCACAACA GCTGTAAAGA GCAGTTGGGG AAGGTTTGTC CTCTGGGTCA GTGTAAGGTC 
TCCATCATTC CACCCACTGC ACTTAACAGC ATCGACTCTG ACGGCTTCTG GAAGGCCACC 
AGTCCATCCT GCTCCAGTCC TCTTCTGGTG CTGGTGAACA GTAAGAGTGG AGACAACCAG 
GGTGTCAAGT TCCTGCGCAG GTTTAAGCAG CTCCTGAATC CAGCGCAGGT CTTTGACCTC 
ATGAATGGCG GCCCACAGCT GGGGCTTCGT CTCTTCCAGA AGTTTGAGAC ATTTCGTACC 
CTGGTGTGCG GTGGAGATGG GAGCGTGGGC TGGGTTCTTT CTGAACTGGA CAAACTCAGC 
CTGCACAAAC AGTGTCAGCT GGGTGTGCTG CCGCTGGGCA CTGGAAATGA CCTGGCGCGT 
GTATTGGGCT GGGGAGGCCT GTGTGACGAC GACGCTCAGC TGCTGCAGAT ACTGGAGAAA 
CTGGAGAGAG CAACAACCAA GATGCTGGAC AGATGGAGTG TGATGACCTA TGAAGTGCCC 
ACCAAACAGA CTCCAGCAAT CGTAAAAGAC GACGACCCAT CAGATTCCCC TCTACAGATT 
ACACAATATG CTGATTCTGT TGCCACTCAC TTGACCAAAA TCCTGGACTC TGACAAACAC 
AGTGACGTTA TTTCATCTGC CAAGTTTCTA TGTGGAACAG TTAATGATTT TGTGGCTGAG 
GTCGGCAAAG CTTATGAACG GGCAACAGAG AACAAGGAGG AGGCCGATGC CATGGCTAAA 
AAGTGTGCCA TGCTGAATGA GAAGCTGGAC TCGTTGGTTA AGGCTCTCAG TGAAGATGCC 
AATGCCCAGC TGGTGCCAGC CGGGTCCATT TCTCCAGACG ACAGCGACAC GGATCAGTCC 
AGACCCTTCA GATCTAGAGA GCAGCTAATG CTGAGGGCCA ACAGCCTGAA GAAAGCACTC 
CGACAAATCA TAGATCAAGC AGAAAAAGTG GTGGATGAGC AGAACAGACA CACACAGGTG 
CAGAGAATGT CTTCCACCTC GTCAATCAAA AGAGGCAGCA GTGAGGACCT CAAGGAGGCG 
GAAACACGTC AAAATACATT GAGTCCGACC GCTACAACTC CTATATTACT GGAGAAGTCA 
GAAAGCTTCG GCACCGTGCT CTTCTCTGAA GAATCTGTGC AGTGCACAGA GAAGTGTGTG 
ATGAATAATT ATTTTGGCAT TGGGCTAGAT GCCAAAATAT CTCTAGAGTT CAACAACAAG 
AGAGACGAGA ACCCCAAAAA ATGCAGTAGT CGCACTAAAA ACATGATGTG GTACGGAGTG 
CTAGGGACCA AAGAACTGGT GCAGAAAACC TACAAGAACC TGGAACAAAG AGTCCAGCTG 
GAGTGTGATG GCGTGTCCAT GTCCTTGCCG AGTCTTCAGG GTCTGGCTGT TTTGAACATC 
CCCAGCTATG CAGGAGGCAT CAATTTCTGG GGAGGCACCA AAGAGGACAA TAATTTTGGA 
GCTCCATCTT TTGATGATAA GAAGCTGGAG GTGGTGGCTG TGTTTGGCAG CATGCAGATG 
GCAATGTCTC GGGTCATCAA CATACAGCAT CACCGCATCG CACAGTGTCG GCAGGTAAAG 
ATCACCATCC TTGGTGATGA AGGTGTTCCT GTGCAGGTGG ATGGTGAGGC CTGGATTCAG 
CCTCCGGGCA TCGTCCAAAT TGTTCACAAG AACCGAGCCC AGATGCTCAC CAGAGACCGG 
GCCTTCGAGA GCACGCTGAA ATCATGGGAG GACAAGCGTA AAGGTGACAG CCGGCCATCT 
CGACCACGAC TCAACTCCCA ACAGTCTATG GAGTATCTGA CGGAGGAGGA ATGTGCTCAG 
GTCCAGCAGC TTGGCTTAGT GGCCGACACG CTCATCAGCA GGATTCGGGA AGTGGCGAAG 
ACTCATAAGG TGGTGGAGCA GGAACTGGCT CATGCTGTAA ATGCCAGCGC TGCTGTCCTG 
AGTGAAGCAT TTCCTTCCAA ACCCTCCAGT CCTGAGGGTT TGAGTCGCAG TACAGCTGTA 
GATATTGTCA ACAGCAGTAC AGTTCTCCAG CAAGAGACCA AGATGCTCCT GGAGGGGAAA 
CTGCTGCAGC TGGAGTCTTC TCAGAAAGAG GAGCTTCTGT CCACAGTGGA GAATCTCAGC 
GCTGAATTAC AGAAGCTGGA GGACATTCAT TGGATCTGTC CCAGCATGCA CTGCTCTGAG 
GAGGAATCAT CCCGTGTGTC TGGAAAAAGC AGCATGAAGC TGAAAATCAT GCCCAAGGGG 
AAGAAAGAGA AGGAAAGGTT ACACAAGCAG CGCTCCAGCA GCTCTCTGTC AGGATCATGG 
GATATCAAAG CCAGCGTATG TGATGTGGAT CCGGCAGGAA ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:dkey-172j4.3 ( XM_009303126.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301401.1 Danio rerio si:dkey-172j4.3 (si:dkey-172j4.3), transcript variant X1, mRNA.

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CloneID	ODa44518
Clone ID Related Accession (Same CDS sequence)	XM_009303126.3 , XM_009303126.2
Accession Version	XM_009303126.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	diacylglycerol kinase delta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303126.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGAGC ATTGTGCGCA GCTGATTTTG CTCAAAGGAG GAGACGAGTG CAGTGAAAAT 
CCTGTCCTCT GCACAGTCAT GGCCTCAAAG TTCAATCCAA ACGTCCTTTA TGTCCGGGAG 
CCCCAGGAGT CCCTGACCTC TCCAGACCCT CAAGTTGAGG CGGGTGACGA ATCATCAGAC 
AGTGACGGGG AGCCTGAAGA GACCTCACAC AAACTCATCC GAAAAGTGTC CACCTCTGGA 
CAGATTCGAG CCAAGAAGAG TGTGAAGGAG GGTCTTCTGC TCAAACAGAC CAGCTCTTTT 
CAGAGGTGGA AGAGGAGGTA TTTCAAACTG CGTGGAAGGA CACTTTATTA TGCCAAAGAT 
TCAAAGTCTC TCATATTTGA TGAGGTGGAC CTGTCAGATG CCAGTGTGGC CGAGACCAGC 
ACCAAGAACA TCAACAACAG TTTTACAGTC ATCACTCCAT TCCGTAAGCT GATGTTGTGT 
GCGGAGAGCA GGAAGGAGAT GGAAGACTGG ATCAGTGCTT TGAAGTCTGT ACAGAAATGG 
GAAACATACG AGGCCAGTCA GTTCAACATG GAGCACTTCT CCGGGATGCA TAACTGGTAC 
GCCTGCTCTC ATGCCCGGCC CACTTTCTGC AACGTGTGTC GAGAGGCTCT GCCGGGTGTT 
ACCTCTCATG GTCTCTCCTG TGAAGTGTGT AAGTTCAAAG CCCACAAGCG CTGTGCCGTA 
CGATCCACCA ATACCTGTAA ATGGACAACT CTGGCATCTA TAGGAAATGA CATCATCGAG 
GATGAGGATG GAGTGTCAAT GCCTCACCAG TGGTTGGAGG GAAACCTGCC GGTCAGCGCT 
AAGTGTGTGG TGTGCGATCG AAACTGCGGC AGCGTCCGGC GACTACAGGA CTGGCGGTGT 
CTCTGGTGCA AAGCCATCGT GCACAACAGC TGTAAAGAGC AGTTGGGGAA GGTTTGTCCT 
CTGGGTCAGT GTAAGGTCTC CATCATTCCA CCCACTGCAC TTAACAGCAT CGACTCTGAC 
GGCTTCTGGA AGGCCACCAG TCCATCCTGC TCCAGTCCTC TTCTGGTGCT GGTGAACAGT 
AAGAGTGGAG ACAACCAGGG TGTCAAGTTC CTGCGCAGGT TTAAGCAGCT CCTGAATCCA 
GCGCAGGTCT TTGACCTCAT GAATGGCGGC CCACAGCTGG GGCTTCGTCT CTTCCAGAAG 
TTTGAGACAT TTCGTACCCT GGTGTGCGGT GGAGATGGGA GCGTGGGCTG GGTTCTTTCT 
GAACTGGACA AACTCAGCCT GCACAAACAG TGTCAGCTGG GTGTGCTGCC GCTGGGCACT 
GGAAATGACC TGGCGCGTGT ATTGGGCTGG GGAGGCCTGT GTGACGACGA CGCTCAGCTG 
CTGCAGATAC TGGAGAAACT GGAGAGAGCA ACAACCAAGA TGCTGGACAG ATGGAGTGTG 
ATGACCTATG AAGTGCCCAC CAAACAGACT CCAGCAATCG TAAAAGACGA CGACCCATCA 
GATTCCCCTC TACAGATTAC ACAATATGCT GATTCTGTTG CCACTCACTT GACCAAAATC 
CTGGACTCTG ACAAACACAG TGACGTTATT TCATCTGCCA AGTTTCTATG TGGAACAGTT 
AATGATTTTG TGGCTGAGGT CGGCAAAGCT TATGAACGGG CAACAGAGAA CAAGGAGGAG 
GCCGATGCCA TGGCTAAAAA GTGTGCCATG CTGAATGAGA AGCTGGACTC GTTGGTTAAG 
GCTCTCAGTG AAGATGCCAA TGCCCAGCTG GTGCCAGCCG GGTCCATTTC TCCAGACGAC 
AGCGACACGG ATCAGTCCAG ACCCTTCAGA TCTAGAGAGC AGCTAATGCT GAGGGCCAAC 
AGCCTGAAGA AAGCACTCCG ACAAATCATA GATCAAGCAG AAAAAGTGGT GGATGAGCAG 
AACAGACACA CACAGGTGCA GAGAATGTCT TCCACCTCGT CAATCAAAAG AGGCAGCAGT 
GAGGACCTCA AGGAGGCGGA AACACGTCAA AATACATTGA GTCCGACCGC TACAACTCCT 
ATATTACTGG AGAAGTCAGA AAGCTTCGGC ACCGTGCTCT TCTCTGAAGA ATCTGTGCAG 
TGCACAGAGA AGTGTGTGAT GAATAATTAT TTTGGCATTG GGCTAGATGC CAAAATATCT 
CTAGAGTTCA ACAACAAGAG AGACGAGAAC CCCAAAAAAT GCAGTAGTCG CACTAAAAAC 
ATGATGTGGT ACGGAGTGCT AGGGACCAAA GAACTGGTGC AGAAAACCTA CAAGAACCTG 
GAACAAAGAG TCCAGCTGGA GTGTGATGGC GTGTCCATGT CCTTGCCGAG TCTTCAGGGT 
CTGGCTGTTT TGAACATCCC CAGCTATGCA GGAGGCATCA ATTTCTGGGG AGGCACCAAA 
GAGGACAATA ATTTTGGAGC TCCATCTTTT GATGATAAGA AGCTGGAGGT GGTGGCTGTG 
TTTGGCAGCA TGCAGATGGC AATGTCTCGG GTCATCAACA TACAGCATCA CCGCATCGCA 
CAGTGTCGGC AGGTAAAGAT CACCATCCTT GGTGATGAAG GTGTTCCTGT GCAGGTGGAT 
GGTGAGGCCT GGATTCAGCC TCCGGGCATC GTCCAAATTG TTCACAAGAA CCGAGCCCAG 
ATGCTCACCA GAGACCGGGC CTTCGAGAGC ACGCTGAAAT CATGGGAGGA CAAGCGTAAA 
GGTGACAGCC GGCCATCTCG ACCACGACTC AACTCCCAAC AGTCTATGGA GTATCTGACG 
GAGGAGGAAT GTGCTCAGGT CCAGCAGCTT GGCTTAGTGG CCGACACGCT CATCAGCAGG 
ATTCGGGAAG TGGCGAAGAC TCATAAGGTG GTGGAGCAGG AACTGGCTCA TGCTGTAAAT 
GCCAGCGCTG CTGTCCTGAG TGAAGCATTT CCTTCCAAAC CCTCCAGTCC TGAGGGTTTG 
AGTCGCAGTA CAGCTGTAGA TATTGTCAAC AGCAGTACAG TTCTCCAGCA AGAGACCAAG 
ATGCTCCTGG AGGGGAAACT GCTGCAGCTG GAGTCTTCTC AGAAAGAGGA GCTTCTGTCC 
ACAGTGGAGA ATCTCAGCGC TGAATTACAG AAGCTGGAGG ACATTCATTG GATCTGTCCC 
AGCATGCACT GCTCTGAGGA GGAATCATCC CGTGTGTCTG GAAAAAGCAG CATGAAGCTG 
AAAATCATGC CCAAGGGGAA GAAAGAGAAG GAAAGGTTAC ACAAGCAGCG CTCCAGCAGC 
TCTCTGTCAG GATCATGGGA TATCAAAGCC AGCGTATGTG ATGTGGATCC GGCAGGAAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301401.1
CDS	20..3322
Translation	MLEHCAQLILLKGGDECSENPVLCTVMASKFNPNVLYVREPQESLTSPDPQVEAGDESSDSDGEPEETSHKLIRKVSTSGQIRAKKSVKEGLLLKQTSSFQRWKRRYFKLRGRTLYYAKDSKSLIFDEVDLSDASVAETSTKNINNSFTVITPFRKLMLCAESRKEMEDWISALKSVQKWETYEASQFNMEHFSGMHNWYACSHARPTFCNVCREALPGVTSHGLSCEVCKFKAHKRCAVRSTNTCKWTTLASIGNDIIEDEDGVSMPHQWLEGNLPVSAKCVVCDRNCGSVRRLQDWRCLWCKAIVHNSCKEQLGKVCPLGQCKVSIIPPTALNSIDSDGFWKATSPSCSSPLLVLVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPQLGLRLFQKFETFRTLVCGGDGSVGWVLSELDKLSLHKQCQLGVLPLGTGNDLARVLGWGGLCDDDAQLLQILEKLERATTKMLDRWSVMTYEVPTKQTPAIVKDDDPSDSPLQITQYADSVATHLTKILDSDKHSDVISSAKFLCGTVNDFVAEVGKAYERATENKEEADAMAKKCAMLNEKLDSLVKALSEDANAQLVPAGSISPDDSDTDQSRPFRSREQLMLRANSLKKALRQIIDQAEKVVDEQNRHTQVQRMSSTSSIKRGSSEDLKEAETRQNTLSPTATTPILLEKSESFGTVLFSEESVQCTEKCVMNNYFGIGLDAKISLEFNNKRDENPKKCSSRTKNMMWYGVLGTKELVQKTYKNLEQRVQLECDGVSMSLPSLQGLAVLNIPSYAGGINFWGGTKEDNNFGAPSFDDKKLEVVAVFGSMQMAMSRVINIQHHRIAQCRQVKITILGDEGVPVQVDGEAWIQPPGIVQIVHKNRAQMLTRDRAFESTLKSWEDKRKGDSRPSRPRLNSQQSMEYLTEEECAQVQQLGLVADTLISRIREVAKTHKVVEQELAHAVNASAAVLSEAFPSKPSSPEGLSRSTAVDIVNSSTVLQQETKMLLEGKLLQLESSQKEELLSTVENLSAELQKLEDIHWICPSMHCSEEESSRVSGKSSMKLKIMPKGKKEKERLHKQRSSSSLSGSWDIKASVCDVDPAGN

Target ORF information:

RefSeq Version	XM_009303126.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:dkey-172j4.3 (si:dkey-172j4.3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303126.3

ORF Insert Sequence:

ATGCTGGAGC ATTGTGCGCA GCTGATTTTG CTCAAAGGAG GAGACGAGTG CAGTGAAAAT 
CCTGTCCTCT GCACAGTCAT GGCCTCAAAG TTCAATCCAA ACGTCCTTTA TGTCCGGGAG 
CCCCAGGAGT CCCTGACCTC TCCAGACCCT CAAGTTGAGG CGGGTGACGA ATCATCAGAC 
AGTGACGGGG AGCCTGAAGA GACCTCACAC AAACTCATCC GAAAAGTGTC CACCTCTGGA 
CAGATTCGAG CCAAGAAGAG TGTGAAGGAG GGTCTTCTGC TCAAACAGAC CAGCTCTTTT 
CAGAGGTGGA AGAGGAGGTA TTTCAAACTG CGTGGAAGGA CACTTTATTA TGCCAAAGAT 
TCAAAGTCTC TCATATTTGA TGAGGTGGAC CTGTCAGATG CCAGTGTGGC CGAGACCAGC 
ACCAAGAACA TCAACAACAG TTTTACAGTC ATCACTCCAT TCCGTAAGCT GATGTTGTGT 
GCGGAGAGCA GGAAGGAGAT GGAAGACTGG ATCAGTGCTT TGAAGTCTGT ACAGAAATGG 
GAAACATACG AGGCCAGTCA GTTCAACATG GAGCACTTCT CCGGGATGCA TAACTGGTAC 
GCCTGCTCTC ATGCCCGGCC CACTTTCTGC AACGTGTGTC GAGAGGCTCT GCCGGGTGTT 
ACCTCTCATG GTCTCTCCTG TGAAGTGTGT AAGTTCAAAG CCCACAAGCG CTGTGCCGTA 
CGATCCACCA ATACCTGTAA ATGGACAACT CTGGCATCTA TAGGAAATGA CATCATCGAG 
GATGAGGATG GAGTGTCAAT GCCTCACCAG TGGTTGGAGG GAAACCTGCC GGTCAGCGCT 
AAGTGTGTGG TGTGCGATCG AAACTGCGGC AGCGTCCGGC GACTACAGGA CTGGCGGTGT 
CTCTGGTGCA AAGCCATCGT GCACAACAGC TGTAAAGAGC AGTTGGGGAA GGTTTGTCCT 
CTGGGTCAGT GTAAGGTCTC CATCATTCCA CCCACTGCAC TTAACAGCAT CGACTCTGAC 
GGCTTCTGGA AGGCCACCAG TCCATCCTGC TCCAGTCCTC TTCTGGTGCT GGTGAACAGT 
AAGAGTGGAG ACAACCAGGG TGTCAAGTTC CTGCGCAGGT TTAAGCAGCT CCTGAATCCA 
GCGCAGGTCT TTGACCTCAT GAATGGCGGC CCACAGCTGG GGCTTCGTCT CTTCCAGAAG 
TTTGAGACAT TTCGTACCCT GGTGTGCGGT GGAGATGGGA GCGTGGGCTG GGTTCTTTCT 
GAACTGGACA AACTCAGCCT GCACAAACAG TGTCAGCTGG GTGTGCTGCC GCTGGGCACT 
GGAAATGACC TGGCGCGTGT ATTGGGCTGG GGAGGCCTGT GTGACGACGA CGCTCAGCTG 
CTGCAGATAC TGGAGAAACT GGAGAGAGCA ACAACCAAGA TGCTGGACAG ATGGAGTGTG 
ATGACCTATG AAGTGCCCAC CAAACAGACT CCAGCAATCG TAAAAGACGA CGACCCATCA 
GATTCCCCTC TACAGATTAC ACAATATGCT GATTCTGTTG CCACTCACTT GACCAAAATC 
CTGGACTCTG ACAAACACAG TGACGTTATT TCATCTGCCA AGTTTCTATG TGGAACAGTT 
AATGATTTTG TGGCTGAGGT CGGCAAAGCT TATGAACGGG CAACAGAGAA CAAGGAGGAG 
GCCGATGCCA TGGCTAAAAA GTGTGCCATG CTGAATGAGA AGCTGGACTC GTTGGTTAAG 
GCTCTCAGTG AAGATGCCAA TGCCCAGCTG GTGCCAGCCG GGTCCATTTC TCCAGACGAC 
AGCGACACGG ATCAGTCCAG ACCCTTCAGA TCTAGAGAGC AGCTAATGCT GAGGGCCAAC 
AGCCTGAAGA AAGCACTCCG ACAAATCATA GATCAAGCAG AAAAAGTGGT GGATGAGCAG 
AACAGACACA CACAGGTGCA GAGAATGTCT TCCACCTCGT CAATCAAAAG AGGCAGCAGT 
GAGGACCTCA AGGAGGCGGA AACACGTCAA AATACATTGA GTCCGACCGC TACAACTCCT 
ATATTACTGG AGAAGTCAGA AAGCTTCGGC ACCGTGCTCT TCTCTGAAGA ATCTGTGCAG 
TGCACAGAGA AGTGTGTGAT GAATAATTAT TTTGGCATTG GGCTAGATGC CAAAATATCT 
CTAGAGTTCA ACAACAAGAG AGACGAGAAC CCCAAAAAAT GCAGTAGTCG CACTAAAAAC 
ATGATGTGGT ACGGAGTGCT AGGGACCAAA GAACTGGTGC AGAAAACCTA CAAGAACCTG 
GAACAAAGAG TCCAGCTGGA GTGTGATGGC GTGTCCATGT CCTTGCCGAG TCTTCAGGGT 
CTGGCTGTTT TGAACATCCC CAGCTATGCA GGAGGCATCA ATTTCTGGGG AGGCACCAAA 
GAGGACAATA ATTTTGGAGC TCCATCTTTT GATGATAAGA AGCTGGAGGT GGTGGCTGTG 
TTTGGCAGCA TGCAGATGGC AATGTCTCGG GTCATCAACA TACAGCATCA CCGCATCGCA 
CAGTGTCGGC AGGTAAAGAT CACCATCCTT GGTGATGAAG GTGTTCCTGT GCAGGTGGAT 
GGTGAGGCCT GGATTCAGCC TCCGGGCATC GTCCAAATTG TTCACAAGAA CCGAGCCCAG 
ATGCTCACCA GAGACCGGGC CTTCGAGAGC ACGCTGAAAT CATGGGAGGA CAAGCGTAAA 
GGTGACAGCC GGCCATCTCG ACCACGACTC AACTCCCAAC AGTCTATGGA GTATCTGACG 
GAGGAGGAAT GTGCTCAGGT CCAGCAGCTT GGCTTAGTGG CCGACACGCT CATCAGCAGG 
ATTCGGGAAG TGGCGAAGAC TCATAAGGTG GTGGAGCAGG AACTGGCTCA TGCTGTAAAT 
GCCAGCGCTG CTGTCCTGAG TGAAGCATTT CCTTCCAAAC CCTCCAGTCC TGAGGGTTTG 
AGTCGCAGTA CAGCTGTAGA TATTGTCAAC AGCAGTACAG TTCTCCAGCA AGAGACCAAG 
ATGCTCCTGG AGGGGAAACT GCTGCAGCTG GAGTCTTCTC AGAAAGAGGA GCTTCTGTCC 
ACAGTGGAGA ATCTCAGCGC TGAATTACAG AAGCTGGAGG ACATTCATTG GATCTGTCCC 
AGCATGCACT GCTCTGAGGA GGAATCATCC CGTGTGTCTG GAAAAAGCAG CATGAAGCTG 
AAAATCATGC CCAAGGGGAA GAAAGAGAAG GAAAGGTTAC ACAAGCAGCG CTCCAGCAGC 
TCTCTGTCAG GATCATGGGA TATCAAAGCC AGCGTATGTG ATGTGGATCC GGCAGGAAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:dkey-172j4.3 ( XM_009303126.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301401.1 Danio rerio si:dkey-172j4.3 (si:dkey-172j4.3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa44518
Clone ID Related Accession (Same CDS sequence)	XM_009303126.3 , XM_009303126.2
Accession Version	XM_009303126.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	diacylglycerol kinase kappa isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303126.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGAGC ATTGTGCGCA GCTGATTTTG CTCAAAGGAG GAGACGAGTG CAGTGAAAAT 
CCTGTCCTCT GCACAGTCAT GGCCTCAAAG TTCAATCCAA ACGTCCTTTA TGTCCGGGAG 
CCCCAGGAGT CCCTGACCTC TCCAGACCCT CAAGTTGAGG CGGGTGACGA ATCATCAGAC 
AGTGACGGGG AGCCTGAAGA GACCTCACAC AAACTCATCC GAAAAGTGTC CACCTCTGGA 
CAGATTCGAG CCAAGAAGAG TGTGAAGGAG GGTCTTCTGC TCAAACAGAC CAGCTCTTTT 
CAGAGGTGGA AGAGGAGGTA TTTCAAACTG CGTGGAAGGA CACTTTATTA TGCCAAAGAT 
TCAAAGTCTC TCATATTTGA TGAGGTGGAC CTGTCAGATG CCAGTGTGGC CGAGACCAGC 
ACCAAGAACA TCAACAACAG TTTTACAGTC ATCACTCCAT TCCGTAAGCT GATGTTGTGT 
GCGGAGAGCA GGAAGGAGAT GGAAGACTGG ATCAGTGCTT TGAAGTCTGT ACAGAAATGG 
GAAACATACG AGGCCAGTCA GTTCAACATG GAGCACTTCT CCGGGATGCA TAACTGGTAC 
GCCTGCTCTC ATGCCCGGCC CACTTTCTGC AACGTGTGTC GAGAGGCTCT GCCGGGTGTT 
ACCTCTCATG GTCTCTCCTG TGAAGTGTGT AAGTTCAAAG CCCACAAGCG CTGTGCCGTA 
CGATCCACCA ATACCTGTAA ATGGACAACT CTGGCATCTA TAGGAAATGA CATCATCGAG 
GATGAGGATG GAGTGTCAAT GCCTCACCAG TGGTTGGAGG GAAACCTGCC GGTCAGCGCT 
AAGTGTGTGG TGTGCGATCG AAACTGCGGC AGCGTCCGGC GACTACAGGA CTGGCGGTGT 
CTCTGGTGCA AAGCCATCGT GCACAACAGC TGTAAAGAGC AGTTGGGGAA GGTTTGTCCT 
CTGGGTCAGT GTAAGGTCTC CATCATTCCA CCCACTGCAC TTAACAGCAT CGACTCTGAC 
GGCTTCTGGA AGGCCACCAG TCCATCCTGC TCCAGTCCTC TTCTGGTGCT GGTGAACAGT 
AAGAGTGGAG ACAACCAGGG TGTCAAGTTC CTGCGCAGGT TTAAGCAGCT CCTGAATCCA 
GCGCAGGTCT TTGACCTCAT GAATGGCGGC CCACAGCTGG GGCTTCGTCT CTTCCAGAAG 
TTTGAGACAT TTCGTACCCT GGTGTGCGGT GGAGATGGGA GCGTGGGCTG GGTTCTTTCT 
GAACTGGACA AACTCAGCCT GCACAAACAG TGTCAGCTGG GTGTGCTGCC GCTGGGCACT 
GGAAATGACC TGGCGCGTGT ATTGGGCTGG GGAGGCCTGT GTGACGACGA CGCTCAGCTG 
CTGCAGATAC TGGAGAAACT GGAGAGAGCA ACAACCAAGA TGCTGGACAG ATGGAGTGTG 
ATGACCTATG AAGTGCCCAC CAAACAGACT CCAGCAATCG TAAAAGACGA CGACCCATCA 
GATTCCCCTC TACAGATTAC ACAATATGCT GATTCTGTTG CCACTCACTT GACCAAAATC 
CTGGACTCTG ACAAACACAG TGACGTTATT TCATCTGCCA AGTTTCTATG TGGAACAGTT 
AATGATTTTG TGGCTGAGGT CGGCAAAGCT TATGAACGGG CAACAGAGAA CAAGGAGGAG 
GCCGATGCCA TGGCTAAAAA GTGTGCCATG CTGAATGAGA AGCTGGACTC GTTGGTTAAG 
GCTCTCAGTG AAGATGCCAA TGCCCAGCTG GTGCCAGCCG GGTCCATTTC TCCAGACGAC 
AGCGACACGG ATCAGTCCAG ACCCTTCAGA TCTAGAGAGC AGCTAATGCT GAGGGCCAAC 
AGCCTGAAGA AAGCACTCCG ACAAATCATA GATCAAGCAG AAAAAGTGGT GGATGAGCAG 
AACAGACACA CACAGGTGCA GAGAATGTCT TCCACCTCGT CAATCAAAAG AGGCAGCAGT 
GAGGACCTCA AGGAGGCGGA AACACGTCAA AATACATTGA GTCCGACCGC TACAACTCCT 
ATATTACTGG AGAAGTCAGA AAGCTTCGGC ACCGTGCTCT TCTCTGAAGA ATCTGTGCAG 
TGCACAGAGA AGTGTGTGAT GAATAATTAT TTTGGCATTG GGCTAGATGC CAAAATATCT 
CTAGAGTTCA ACAACAAGAG AGACGAGAAC CCCAAAAAAT GCAGTAGTCG CACTAAAAAC 
ATGATGTGGT ACGGAGTGCT AGGGACCAAA GAACTGGTGC AGAAAACCTA CAAGAACCTG 
GAACAAAGAG TCCAGCTGGA GTGTGATGGC GTGTCCATGT CCTTGCCGAG TCTTCAGGGT 
CTGGCTGTTT TGAACATCCC CAGCTATGCA GGAGGCATCA ATTTCTGGGG AGGCACCAAA 
GAGGACAATA ATTTTGGAGC TCCATCTTTT GATGATAAGA AGCTGGAGGT GGTGGCTGTG 
TTTGGCAGCA TGCAGATGGC AATGTCTCGG GTCATCAACA TACAGCATCA CCGCATCGCA 
CAGTGTCGGC AGGTAAAGAT CACCATCCTT GGTGATGAAG GTGTTCCTGT GCAGGTGGAT 
GGTGAGGCCT GGATTCAGCC TCCGGGCATC GTCCAAATTG TTCACAAGAA CCGAGCCCAG 
ATGCTCACCA GAGACCGGGC CTTCGAGAGC ACGCTGAAAT CATGGGAGGA CAAGCGTAAA 
GGTGACAGCC GGCCATCTCG ACCACGACTC AACTCCCAAC AGTCTATGGA GTATCTGACG 
GAGGAGGAAT GTGCTCAGGT CCAGCAGCTT GGCTTAGTGG CCGACACGCT CATCAGCAGG 
ATTCGGGAAG TGGCGAAGAC TCATAAGGTG GTGGAGCAGG AACTGGCTCA TGCTGTAAAT 
GCCAGCGCTG CTGTCCTGAG TGAAGCATTT CCTTCCAAAC CCTCCAGTCC TGAGGGTTTG 
AGTCGCAGTA CAGCTGTAGA TATTGTCAAC AGCAGTACAG TTCTCCAGCA AGAGACCAAG 
ATGCTCCTGG AGGGGAAACT GCTGCAGCTG GAGTCTTCTC AGAAAGAGGA GCTTCTGTCC 
ACAGTGGAGA ATCTCAGCGC TGAATTACAG AAGCTGGAGG ACATTCATTG GATCTGTCCC 
AGCATGCACT GCTCTGAGGA GGAATCATCC CGTGTGTCTG GAAAAAGCAG CATGAAGCTG 
AAAATCATGC CCAAGGGGAA GAAAGAGAAG GAAAGGTTAC ACAAGCAGCG CTCCAGCAGC 
TCTCTGTCAG GATCATGGGA TATCAAAGCC AGCGTATGTG ATGTGGATCC GGCAGGAAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301401.1
CDS	46..3348
Translation	MLEHCAQLILLKGGDECSENPVLCTVMASKFNPNVLYVREPQESLTSPDPQVEAGDESSDSDGEPEETSHKLIRKVSTSGQIRAKKSVKEGLLLKQTSSFQRWKRRYFKLRGRTLYYAKDSKSLIFDEVDLSDASVAETSTKNINNSFTVITPFRKLMLCAESRKEMEDWISALKSVQKWETYEASQFNMEHFSGMHNWYACSHARPTFCNVCREALPGVTSHGLSCEVCKFKAHKRCAVRSTNTCKWTTLASIGNDIIEDEDGVSMPHQWLEGNLPVSAKCVVCDRNCGSVRRLQDWRCLWCKAIVHNSCKEQLGKVCPLGQCKVSIIPPTALNSIDSDGFWKATSPSCSSPLLVLVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPQLGLRLFQKFETFRTLVCGGDGSVGWVLSELDKLSLHKQCQLGVLPLGTGNDLARVLGWGGLCDDDAQLLQILEKLERATTKMLDRWSVMTYEVPTKQTPAIVKDDDPSDSPLQITQYADSVATHLTKILDSDKHSDVISSAKFLCGTVNDFVAEVGKAYERATENKEEADAMAKKCAMLNEKLDSLVKALSEDANAQLVPAGSISPDDSDTDQSRPFRSREQLMLRANSLKKALRQIIDQAEKVVDEQNRHTQVQRMSSTSSIKRGSSEDLKEAETRQNTLSPTATTPILLEKSESFGTVLFSEESVQCTEKCVMNNYFGIGLDAKISLEFNNKRDENPKKCSSRTKNMMWYGVLGTKELVQKTYKNLEQRVQLECDGVSMSLPSLQGLAVLNIPSYAGGINFWGGTKEDNNFGAPSFDDKKLEVVAVFGSMQMAMSRVINIQHHRIAQCRQVKITILGDEGVPVQVDGEAWIQPPGIVQIVHKNRAQMLTRDRAFESTLKSWEDKRKGDSRPSRPRLNSQQSMEYLTEEECAQVQQLGLVADTLISRIREVAKTHKVVEQELAHAVNASAAVLSEAFPSKPSSPEGLSRSTAVDIVNSSTVLQQETKMLLEGKLLQLESSQKEELLSTVENLSAELQKLEDIHWICPSMHCSEEESSRVSGKSSMKLKIMPKGKKEKERLHKQRSSSSLSGSWDIKASVCDVDPAGN

Target ORF information:

RefSeq Version	XM_009303126.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:dkey-172j4.3 (si:dkey-172j4.3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303126.2

ORF Insert Sequence:

ATGCTGGAGC ATTGTGCGCA GCTGATTTTG CTCAAAGGAG GAGACGAGTG CAGTGAAAAT 
CCTGTCCTCT GCACAGTCAT GGCCTCAAAG TTCAATCCAA ACGTCCTTTA TGTCCGGGAG 
CCCCAGGAGT CCCTGACCTC TCCAGACCCT CAAGTTGAGG CGGGTGACGA ATCATCAGAC 
AGTGACGGGG AGCCTGAAGA GACCTCACAC AAACTCATCC GAAAAGTGTC CACCTCTGGA 
CAGATTCGAG CCAAGAAGAG TGTGAAGGAG GGTCTTCTGC TCAAACAGAC CAGCTCTTTT 
CAGAGGTGGA AGAGGAGGTA TTTCAAACTG CGTGGAAGGA CACTTTATTA TGCCAAAGAT 
TCAAAGTCTC TCATATTTGA TGAGGTGGAC CTGTCAGATG CCAGTGTGGC CGAGACCAGC 
ACCAAGAACA TCAACAACAG TTTTACAGTC ATCACTCCAT TCCGTAAGCT GATGTTGTGT 
GCGGAGAGCA GGAAGGAGAT GGAAGACTGG ATCAGTGCTT TGAAGTCTGT ACAGAAATGG 
GAAACATACG AGGCCAGTCA GTTCAACATG GAGCACTTCT CCGGGATGCA TAACTGGTAC 
GCCTGCTCTC ATGCCCGGCC CACTTTCTGC AACGTGTGTC GAGAGGCTCT GCCGGGTGTT 
ACCTCTCATG GTCTCTCCTG TGAAGTGTGT AAGTTCAAAG CCCACAAGCG CTGTGCCGTA 
CGATCCACCA ATACCTGTAA ATGGACAACT CTGGCATCTA TAGGAAATGA CATCATCGAG 
GATGAGGATG GAGTGTCAAT GCCTCACCAG TGGTTGGAGG GAAACCTGCC GGTCAGCGCT 
AAGTGTGTGG TGTGCGATCG AAACTGCGGC AGCGTCCGGC GACTACAGGA CTGGCGGTGT 
CTCTGGTGCA AAGCCATCGT GCACAACAGC TGTAAAGAGC AGTTGGGGAA GGTTTGTCCT 
CTGGGTCAGT GTAAGGTCTC CATCATTCCA CCCACTGCAC TTAACAGCAT CGACTCTGAC 
GGCTTCTGGA AGGCCACCAG TCCATCCTGC TCCAGTCCTC TTCTGGTGCT GGTGAACAGT 
AAGAGTGGAG ACAACCAGGG TGTCAAGTTC CTGCGCAGGT TTAAGCAGCT CCTGAATCCA 
GCGCAGGTCT TTGACCTCAT GAATGGCGGC CCACAGCTGG GGCTTCGTCT CTTCCAGAAG 
TTTGAGACAT TTCGTACCCT GGTGTGCGGT GGAGATGGGA GCGTGGGCTG GGTTCTTTCT 
GAACTGGACA AACTCAGCCT GCACAAACAG TGTCAGCTGG GTGTGCTGCC GCTGGGCACT 
GGAAATGACC TGGCGCGTGT ATTGGGCTGG GGAGGCCTGT GTGACGACGA CGCTCAGCTG 
CTGCAGATAC TGGAGAAACT GGAGAGAGCA ACAACCAAGA TGCTGGACAG ATGGAGTGTG 
ATGACCTATG AAGTGCCCAC CAAACAGACT CCAGCAATCG TAAAAGACGA CGACCCATCA 
GATTCCCCTC TACAGATTAC ACAATATGCT GATTCTGTTG CCACTCACTT GACCAAAATC 
CTGGACTCTG ACAAACACAG TGACGTTATT TCATCTGCCA AGTTTCTATG TGGAACAGTT 
AATGATTTTG TGGCTGAGGT CGGCAAAGCT TATGAACGGG CAACAGAGAA CAAGGAGGAG 
GCCGATGCCA TGGCTAAAAA GTGTGCCATG CTGAATGAGA AGCTGGACTC GTTGGTTAAG 
GCTCTCAGTG AAGATGCCAA TGCCCAGCTG GTGCCAGCCG GGTCCATTTC TCCAGACGAC 
AGCGACACGG ATCAGTCCAG ACCCTTCAGA TCTAGAGAGC AGCTAATGCT GAGGGCCAAC 
AGCCTGAAGA AAGCACTCCG ACAAATCATA GATCAAGCAG AAAAAGTGGT GGATGAGCAG 
AACAGACACA CACAGGTGCA GAGAATGTCT TCCACCTCGT CAATCAAAAG AGGCAGCAGT 
GAGGACCTCA AGGAGGCGGA AACACGTCAA AATACATTGA GTCCGACCGC TACAACTCCT 
ATATTACTGG AGAAGTCAGA AAGCTTCGGC ACCGTGCTCT TCTCTGAAGA ATCTGTGCAG 
TGCACAGAGA AGTGTGTGAT GAATAATTAT TTTGGCATTG GGCTAGATGC CAAAATATCT 
CTAGAGTTCA ACAACAAGAG AGACGAGAAC CCCAAAAAAT GCAGTAGTCG CACTAAAAAC 
ATGATGTGGT ACGGAGTGCT AGGGACCAAA GAACTGGTGC AGAAAACCTA CAAGAACCTG 
GAACAAAGAG TCCAGCTGGA GTGTGATGGC GTGTCCATGT CCTTGCCGAG TCTTCAGGGT 
CTGGCTGTTT TGAACATCCC CAGCTATGCA GGAGGCATCA ATTTCTGGGG AGGCACCAAA 
GAGGACAATA ATTTTGGAGC TCCATCTTTT GATGATAAGA AGCTGGAGGT GGTGGCTGTG 
TTTGGCAGCA TGCAGATGGC AATGTCTCGG GTCATCAACA TACAGCATCA CCGCATCGCA 
CAGTGTCGGC AGGTAAAGAT CACCATCCTT GGTGATGAAG GTGTTCCTGT GCAGGTGGAT 
GGTGAGGCCT GGATTCAGCC TCCGGGCATC GTCCAAATTG TTCACAAGAA CCGAGCCCAG 
ATGCTCACCA GAGACCGGGC CTTCGAGAGC ACGCTGAAAT CATGGGAGGA CAAGCGTAAA 
GGTGACAGCC GGCCATCTCG ACCACGACTC AACTCCCAAC AGTCTATGGA GTATCTGACG 
GAGGAGGAAT GTGCTCAGGT CCAGCAGCTT GGCTTAGTGG CCGACACGCT CATCAGCAGG 
ATTCGGGAAG TGGCGAAGAC TCATAAGGTG GTGGAGCAGG AACTGGCTCA TGCTGTAAAT 
GCCAGCGCTG CTGTCCTGAG TGAAGCATTT CCTTCCAAAC CCTCCAGTCC TGAGGGTTTG 
AGTCGCAGTA CAGCTGTAGA TATTGTCAAC AGCAGTACAG TTCTCCAGCA AGAGACCAAG 
ATGCTCCTGG AGGGGAAACT GCTGCAGCTG GAGTCTTCTC AGAAAGAGGA GCTTCTGTCC 
ACAGTGGAGA ATCTCAGCGC TGAATTACAG AAGCTGGAGG ACATTCATTG GATCTGTCCC 
AGCATGCACT GCTCTGAGGA GGAATCATCC CGTGTGTCTG GAAAAAGCAG CATGAAGCTG 
AAAATCATGC CCAAGGGGAA GAAAGAGAAG GAAAGGTTAC ACAAGCAGCG CTCCAGCAGC 
TCTCTGTCAG GATCATGGGA TATCAAAGCC AGCGTATGTG ATGTGGATCC GGCAGGAAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	si:dkey-172j4.3
Entrez Gene ID	565122
Full Name	si:dkey-172j4.3
General protein information	Preferred Names si:dkey-172j4.3 Names diacylglycerol kinase delta diacylglycerol kinase family
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Danio rerio (zebrafish)	si:dkey-172j4.3	XP_005172372.1
Gallus gallus (chicken)	DGKK	XP_420132.4
Xenopus tropicalis (tropical clawed frog)	LOC100496859	XP_004916837.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

si:dkey-172j4.3 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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