capn1b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	capn1b
Entrez Gene ID	565106
Full Name	calpain 1, (mu/I) large subunit b
Synonyms	si:ch211-203k12.4,si:dkeyp-101g1.3
General protein information	Preferred Names calpain 1, (mu/I) large subunit b Names calpain 1, large subunit
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009296390.2	XP_009294665.1	calpain 1, large subunit isoform X1
NM_001144795.1	NP_001138267.1	calpain 1, large subunit
XM_005174031.3	XP_005174088.1	calpain 1, large subunit isoform X1
XM_005174031.4	XP_005174088.1	calpain 1, large subunit isoform X1

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	capn1	NP_001013631.1
Danio rerio (zebrafish)	capn1a	NP_956739.1
Danio rerio (zebrafish)	capn1b	NP_001138267.1
Gallus gallus (chicken)	CAPN1	NP_990634.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Mutations in CAPN1 Cause Autosomal-Recessive Hereditary Spastic Paraplegia.
Gan-Or Z, Bouslam N, Birouk N, Lissouba A, Chambers DB, V?ri?pe J, Androschuk A, Laurent SB, Rochefort D, Spiegelman D, Dionne-Laporte A, Szuto A, Liao M, Figlewicz DA, Bouhouche A, Benomar A, Yahyaoui M, Ouazzani R, Yoon G, Dupr? N, Suchowersky O, Bolduc FV, Parker JA, Dion PA, Drapeau P, Rouleau GA, Ouled Amar Bencheikh B
American journal of human genetics98(5)1038-1046(2016 May)

Phenotype anchoring in zebrafish reveals a potential role for matrix metalloproteinases (MMPs) in tamoxifen's effects on skin epithelium.
Bugel SM, Wehmas LC, La Du JK, Tanguay RL
Toxicology and applied pharmacology29631-41(2016 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following capn1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the capn1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa31237	NM_001144795.1 Latest version!	Danio rerio calpain 1, (mu/I) large subunit b (capn1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa27025	XM_009296390.2 Latest version!	Danio rerio calpain 1, (mu/I) large subunit b (capn1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa27025	XM_005174031.4 Latest version!	Danio rerio calpain 1, (mu/I) large subunit b (capn1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
ODa27025	XM_005174031.3	Danio rerio calpain 1, (mu/I) large subunit b (capn1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

capn1b ( NM_001144795.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001138267.1 Danio rerio calpain 1, (mu/I) large subunit b (capn1b), mRNA.

Price & Availability↑

CloneID	ODa31237
Clone ID Related Accession (Same CDS sequence)	NM_001144795.1
Accession Version	NM_001144795.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2103bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-17
Organism	Danio rerio(zebrafish)
Product	calpain 1, large subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX649315.28. On Jan 25, 2009 this sequence version replaced XM_688403.2. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTTTTCAA TTGGAGGTGT GTCGACGCGC ATCTACAAGG AGCGCCTGCA GGCAGAAGGG 
ATGGGAGCGA ATGACTTGGC CATCAAGTTC CTGAACCAGG ACTATGAGGA GCTGAGAAGG 
GAGTGTGTGG AGAGTGGCAG GCTGTTTGAG GACCCTTGCT TTCCTGCAGT GCCCCAATCA 
CTGGGCTTCA AGGAGCTCGC GCCAAACTCC TCCAAAACAC GTGGTGTGAA GTGGATAAGA 
CCCACTGAGC TGTCAGAGAA TCCTCAGTTT ATAGTGGGCG GAGCCACGCG TACAGATATT 
TGCCAGGGGG CCCTAGGTGA CTGTTGGCTT TTGGCTGCCA TAGCGTCGTT GACATTAAAC 
GACAAGCTGC TTCACAGAGT TGTACCACAT GGGCAAAGCT TTACAAATGA GTATGCTGGA 
ATCTTCCACT TCCAGTTCTG GCAGTTTGGC GAGTGGGTAG ACATTGTAAT CGATGACCGC 
TTGCCTGTGA AGGATAAAGA GCTGATGTTT GTTCACTCAG CAGAGGGAAA TGAGTTCTGG 
AGCGCCCTGC TGGAGAAGGC ATATGCTAAG CTAAATGGCT CATATGAAGC TCTGTCCGGA 
GGTTCCACCA CCGAAGGCTT TGAGGACTTT ACTGGCGGAG TTGCCGAAAT GTATGAACTG 
CGCAGCGCTC CCAAAGACCT GCACCGCATC ATTGCCAAAG CCCTGGAGAG AGGCTCACTG 
CTGGGCTGCT CCATTGATAT CACCAGTGCC TTTGACATGG AGGCTGTGAC CTTTAAGAAG 
CTAGTGAAGG GCCATGCTTA CTCCGTCACA GCTCTCAGAG AGGTGAATTT CCGTGGCAAC 
AGAGAGAGAT TGATCCGTAT CCGAAACCCT TGGGGACAAG TTGAATGGAC TGGGGCTTGG 
AGCGACAATT CGTCCGAGTG GAATGGGATT GATCCATCGG AAAGAGAGGA ACTGAACAAC 
CACATGGAGG ATGGCGAGTT TTGGATGTCA TTTCAGGAGT TCAAACGTCA GTTTTCTCGT 
CTGGAGATCT GCAACCTCAC TCCTGATGCT CTGAGTGATG ATTCCCAACA TTTCTGGAAC 
ACAATTCAGT TTAACGGCAC TTGGAGGAAA GGAAGTACAG CAGGAGGCTG CAGGAACAAT 
CCCAACACAT TCTGGATAAA CCCTCAATAT AAGATCACAC TACTGGAGGA AGATGATGAT 
CCTGAGGACA ATGAGGTGGC CTGCACCTTC ATGGTGGCTT TGATGCAGAA GGACAGACGG 
CGTTTCCGCA AGCAGGGACA GGACATGCAC ACCATCGGCT TCGCCTTATA TGAGTTTCTT 
GGCTCCCAGA ATGTTCACCT GAAGAAGGAT TTTTTCTTGC GTCACTCGTC CTGTGCACGC 
TCCGAGACCT TTATTAATCT GCGAGAGGTG AGCACGCGAC TGAGGCTGCC ACCGGGCGAG 
TATCTCATTG TCCCTTCCAC TTTTGAGCCT GGAAAAGAGG CTGACTTCGT CCTGCGAGTA 
TTCACTGAGA AACAGTCAGA AACTCAAGAA ATGGATGATG AAATTTCATT TAACCTGGAA 
GATGAAGAGG CAGTCTCAGA GGAGAACATT GATGCCTCGT TTAAAAAGAT GTTTGCTCAG 
CTGTCTGGAG AGGACATGGA GATTTCAGTT CACGAGCTCC GGACCATTCT GAACCGAGTG 
ATGACAAAAC ACCGAGACTT AAAGACAGAT GGGTTCAGTT TGGAGTCTTG TAGGTGCATG 
ATCAACCTGA TGGACAAAGA CGGCAGTGCA CGACTGGGAA TTGTGGAGTT CCAGATTCTG 
TGGAATAAAA TCAGGAAGTG GCTGGGTGTC TTCAGACAGT TCGATCTGGA TAAATCTGGA 
ACTATGAGCT CCTACGAAAT GCGCCTGGCT GTGGAGTCTG CAGGTTTTAA ACTCAATAAC 
AAGCTACATC AGATCCTGGT GGCCCGATAT TCAGACGGAG ATGTGATTGA CTTCGACAAC 
TTTGTCTGCT GCCTGGTTAA ACTGGAGGCC ATGTTCAAGT CTTTCAAAGA GCTGGAGAAA 
GAGGGCTCAG GTGTCGCAGA GCTCAATATT AGCGAGGTCA GTCATTTCTT ACTTTCTAAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138267.1
CDS	1..2103
Translation	MFSIGGVSTRIYKERLQAEGMGANDLAIKFLNQDYEELRRECVESGRLFEDPCFPAVPQSLGFKELAPNSSKTRGVKWIRPTELSENPQFIVGGATRTDICQGALGDCWLLAAIASLTLNDKLLHRVVPHGQSFTNEYAGIFHFQFWQFGEWVDIVIDDRLPVKDKELMFVHSAEGNEFWSALLEKAYAKLNGSYEALSGGSTTEGFEDFTGGVAEMYELRSAPKDLHRIIAKALERGSLLGCSIDITSAFDMEAVTFKKLVKGHAYSVTALREVNFRGNRERLIRIRNPWGQVEWTGAWSDNSSEWNGIDPSEREELNNHMEDGEFWMSFQEFKRQFSRLEICNLTPDALSDDSQHFWNTIQFNGTWRKGSTAGGCRNNPNTFWINPQYKITLLEEDDDPEDNEVACTFMVALMQKDRRRFRKQGQDMHTIGFALYEFLGSQNVHLKKDFFLRHSSCARSETFINLREVSTRLRLPPGEYLIVPSTFEPGKEADFVLRVFTEKQSETQEMDDEISFNLEDEEAVSEENIDASFKKMFAQLSGEDMEISVHELRTILNRVMTKHRDLKTDGFSLESCRCMINLMDKDGSARLGIVEFQILWNKIRKWLGVFRQFDLDKSGTMSSYEMRLAVESAGFKLNNKLHQILVARYSDGDVIDFDNFVCCLVKLEAMFKSFKELEKEGSGVAELNISEVSHFLLSK

Target ORF information:

RefSeq Version	NM_001144795.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpain 1, (mu/I) large subunit b (capn1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144795.1

ORF Insert Sequence:

ATGTTTTCAA TTGGAGGTGT GTCGACGCGC ATCTACAAGG AGCGCCTGCA GGCAGAAGGG 
ATGGGAGCGA ATGACTTGGC CATCAAGTTC CTGAACCAGG ACTATGAGGA GCTGAGAAGG 
GAGTGTGTGG AGAGTGGCAG GCTGTTTGAG GACCCTTGCT TTCCTGCAGT GCCCCAATCA 
CTGGGCTTCA AGGAGCTCGC GCCAAACTCC TCCAAAACAC GTGGTGTGAA GTGGATAAGA 
CCCACTGAGC TGTCAGAGAA TCCTCAGTTT ATAGTGGGCG GAGCCACGCG TACAGATATT 
TGCCAGGGGG CCCTAGGTGA CTGTTGGCTT TTGGCTGCCA TAGCGTCGTT GACATTAAAC 
GACAAGCTGC TTCACAGAGT TGTACCACAT GGGCAAAGCT TTACAAATGA GTATGCTGGA 
ATCTTCCACT TCCAGTTCTG GCAGTTTGGC GAGTGGGTAG ACATTGTAAT CGATGACCGC 
TTGCCTGTGA AGGATAAAGA GCTGATGTTT GTTCACTCAG CAGAGGGAAA TGAGTTCTGG 
AGCGCCCTGC TGGAGAAGGC ATATGCTAAG CTAAATGGCT CATATGAAGC TCTGTCCGGA 
GGTTCCACCA CCGAAGGCTT TGAGGACTTT ACTGGCGGAG TTGCCGAAAT GTATGAACTG 
CGCAGCGCTC CCAAAGACCT GCACCGCATC ATTGCCAAAG CCCTGGAGAG AGGCTCACTG 
CTGGGCTGCT CCATTGATAT CACCAGTGCC TTTGACATGG AGGCTGTGAC CTTTAAGAAG 
CTAGTGAAGG GCCATGCTTA CTCCGTCACA GCTCTCAGAG AGGTGAATTT CCGTGGCAAC 
AGAGAGAGAT TGATCCGTAT CCGAAACCCT TGGGGACAAG TTGAATGGAC TGGGGCTTGG 
AGCGACAATT CGTCCGAGTG GAATGGGATT GATCCATCGG AAAGAGAGGA ACTGAACAAC 
CACATGGAGG ATGGCGAGTT TTGGATGTCA TTTCAGGAGT TCAAACGTCA GTTTTCTCGT 
CTGGAGATCT GCAACCTCAC TCCTGATGCT CTGAGTGATG ATTCCCAACA TTTCTGGAAC 
ACAATTCAGT TTAACGGCAC TTGGAGGAAA GGAAGTACAG CAGGAGGCTG CAGGAACAAT 
CCCAACACAT TCTGGATAAA CCCTCAATAT AAGATCACAC TACTGGAGGA AGATGATGAT 
CCTGAGGACA ATGAGGTGGC CTGCACCTTC ATGGTGGCTT TGATGCAGAA GGACAGACGG 
CGTTTCCGCA AGCAGGGACA GGACATGCAC ACCATCGGCT TCGCCTTATA TGAGTTTCTT 
GGCTCCCAGA ATGTTCACCT GAAGAAGGAT TTTTTCTTGC GTCACTCGTC CTGTGCACGC 
TCCGAGACCT TTATTAATCT GCGAGAGGTG AGCACGCGAC TGAGGCTGCC ACCGGGCGAG 
TATCTCATTG TCCCTTCCAC TTTTGAGCCT GGAAAAGAGG CTGACTTCGT CCTGCGAGTA 
TTCACTGAGA AACAGTCAGA AACTCAAGAA ATGGATGATG AAATTTCATT TAACCTGGAA 
GATGAAGAGG CAGTCTCAGA GGAGAACATT GATGCCTCGT TTAAAAAGAT GTTTGCTCAG 
CTGTCTGGAG AGGACATGGA GATTTCAGTT CACGAGCTCC GGACCATTCT GAACCGAGTG 
ATGACAAAAC ACCGAGACTT AAAGACAGAT GGGTTCAGTT TGGAGTCTTG TAGGTGCATG 
ATCAACCTGA TGGACAAAGA CGGCAGTGCA CGACTGGGAA TTGTGGAGTT CCAGATTCTG 
TGGAATAAAA TCAGGAAGTG GCTGGGTGTC TTCAGACAGT TCGATCTGGA TAAATCTGGA 
ACTATGAGCT CCTACGAAAT GCGCCTGGCT GTGGAGTCTG CAGGTTTTAA ACTCAATAAC 
AAGCTACATC AGATCCTGGT GGCCCGATAT TCAGACGGAG ATGTGATTGA CTTCGACAAC 
TTTGTCTGCT GCCTGGTTAA ACTGGAGGCC ATGTTCAAGT CTTTCAAAGA GCTGGAGAAA 
GAGGGCTCAG GTGTCGCAGA GCTCAATATT AGCGAGGTCA GTCATTTCTT ACTTTCTAAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

capn1b ( XM_009296390.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009294665.1 Danio rerio calpain 1, (mu/I) large subunit b (capn1b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa27025
Clone ID Related Accession (Same CDS sequence)	XM_009296390.2 , XM_005174031.3 , XM_005174031.4
Accession Version	XM_009296390.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	calpain 1, large subunit isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296390.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTCAA TTGGAGGTGT GTCGACGCGC ATCTACAAGG AGCGCCTGCA GGCAGAAGGG 
ATGGGAGCGA ATGACTTGGC CATCAAGTTC CTGAACCAGG ACTATGAGGA GCTGAGAAGG 
GAGTGTGTGG AGAGTGGCAG GCTGTTTGAG GACCCTTGCT TTCCTGCAGT GCCCCAATCA 
CTGGGCTTCA AGGAGCTCGC GCCAAACTCC TCCAAAACAC GTGGTGTGAA GTGGATAAGA 
CCCACTGAGC TGTCAGAGAA TCCTCAGTTT ATAGTGGGCG GAGCCACGCG TACAGATATT 
TGCCAGGGGG CCCTAGGTGA CTGTTGGCTT TTGGCTGCCA TAGCGTCGTT GACATTAAAC 
GACAAGCTGC TTCACAGAGT TGTACCACAT GGGCAAAGCT TTACAAATGA GTATGCTGGA 
ATCTTCCACT TCCAGTTCTG GCAGTTTGGC GAGTGGGTAG ACATTGTAAT CGATGACCGC 
TTGCCTGTGA AGGATAAAGA GCTGATGTTT GTTCACTCAG CAGAGGGAAA TGAGTTCTGG 
AGCGCCCTGC TGGAGAAGGC ATATGCTAAG CTAAATGGCT CATATGAAGC TCTGTCCGGA 
GGTTCCACCA CCGAAGGCTT TGAGGACTTT ACTGGCGGAG TTGCCGAAAT GTATGAACTG 
CGCAGCGCTC CCAAAGACCT GCACCGCATC ATTGCCAAAG CCCTGGAGAG AGGCTCACTG 
CTGGGCTGCT CCATTGATAT CACCAGTGCC TTTGACATGG AGGCTGTGAC CTTTAAGAAG 
CTAGTGAAGG GCCATGCTTA CTCCGTCACA GCTCTCAGAG AGGTGAATTT CCGTGGCAAC 
AGAGAGAGAT TGATCCGTAT CCGAAACCCT TGGGGACAAG TTGAATGGAC TGGGGCTTGG 
AGCGACAATT CGTCCGAGTG GAATGGGATT GATCCATCGG AAAGAGAGGA ACTGAACAAC 
CACATGGAGG ATGGCGAGTT TTGGATGTCA TTTCAGGAGT TCAAACGTCA GTTTTCTCGT 
CTGGAGATCT GCAACCTCAC TCCTGATGCT CTGAGTGATG ATTCCCAACA TTTCTGGAAC 
ACAATTCAGT TTAACGGCAC TTGGAGGAAA GGAAGTACAG CAGGAGGCTG CAGGAACAAT 
CCCAACACAT TCTGGATAAA CCCTCAATAT AAGATCACAC TACTGGAGGA AGATGATGAT 
CCTGAGGACA ATGAGGTGGC CTGCACCTTC ATGGTGGCTT TGATGCAGAA GGACAGACGG 
CGTTTCCGCA AGCAGGGACA GGACATGCAC ACCATCGGCT TCGCCTTATA TGAGGTCCCA 
GATGAGTTTC TTGGCTCCCA GAATGTTCAC CTGAAGAAGG ATTTTTTCTT GCGTCACTCG 
TCCTGTGCAC GCTCCGAGAC CTTTATTAAT CTGCGAGAGG TGAGCACGCG ACTGAGGCTG 
CCACCGGGCG AGTATCTCAT TGTCCCTTCC ACTTTTGAGC CTGGAAAAGA GGCTGACTTC 
GTCCTGCGAG TATTCACTGA GAAACAGTCA GAAACTCAAG AAATGGATGA TGAAATTTCA 
TTTAACCTGG AAGATGAAGA GGCAGTCTCA GAGGAGAACA TTGATGCCTC GTTTAAAAAG 
ATGTTTGCTC AGCTGTCTGG AGAGGACATG GAGATTTCAG TTCACGAGCT CCGGACCATT 
CTGAACCGAG TGATGACAAA ACACCGAGAC TTAAAGACAG ATGGGTTCAG TTTGGAGTCT 
TGTAGGTGCA TGATCAACCT GATGGACAAA GACGGCAGTG CACGACTGGG AATTGTGGAG 
TTCCAGATTC TGTGGAATAA AATCAGGAAG TGGCTGGGTG TCTTCAGACA GTTCGATCTG 
GATAAATCTG GAACTATGAG CTCCTACGAA ATGCGCCTGG CTGTGGAGTC TGCAGGTTTT 
AAACTCAATA ACAAGCTACA TCAGATCCTG GTGGCCCGAT ATTCAGACGG AGATGTGATT 
GACTTCGACA ACTTTGTCTG CTGCCTGGTT AAACTGGAGG CCATGTTCAA GTCTTTCAAA 
GAGCTGGAGA AAGAGGGCTC AGGTGTCGCA GAGCTCAATA TTAGCGAGTG GCTGTATTTG 
ACCATGTGCG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294665.1
CDS	200..2314
Translation	MFSIGGVSTRIYKERLQAEGMGANDLAIKFLNQDYEELRRECVESGRLFEDPCFPAVPQSLGFKELAPNSSKTRGVKWIRPTELSENPQFIVGGATRTDICQGALGDCWLLAAIASLTLNDKLLHRVVPHGQSFTNEYAGIFHFQFWQFGEWVDIVIDDRLPVKDKELMFVHSAEGNEFWSALLEKAYAKLNGSYEALSGGSTTEGFEDFTGGVAEMYELRSAPKDLHRIIAKALERGSLLGCSIDITSAFDMEAVTFKKLVKGHAYSVTALREVNFRGNRERLIRIRNPWGQVEWTGAWSDNSSEWNGIDPSEREELNNHMEDGEFWMSFQEFKRQFSRLEICNLTPDALSDDSQHFWNTIQFNGTWRKGSTAGGCRNNPNTFWINPQYKITLLEEDDDPEDNEVACTFMVALMQKDRRRFRKQGQDMHTIGFALYEVPDEFLGSQNVHLKKDFFLRHSSCARSETFINLREVSTRLRLPPGEYLIVPSTFEPGKEADFVLRVFTEKQSETQEMDDEISFNLEDEEAVSEENIDASFKKMFAQLSGEDMEISVHELRTILNRVMTKHRDLKTDGFSLESCRCMINLMDKDGSARLGIVEFQILWNKIRKWLGVFRQFDLDKSGTMSSYEMRLAVESAGFKLNNKLHQILVARYSDGDVIDFDNFVCCLVKLEAMFKSFKELEKEGSGVAELNISEWLYLTMCG

Target ORF information:

RefSeq Version	XM_009296390.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calpain 1, (mu/I) large subunit b (capn1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296390.2

ORF Insert Sequence:

ATGTTTTCAA TTGGAGGTGT GTCGACGCGC ATCTACAAGG AGCGCCTGCA GGCAGAAGGG 
ATGGGAGCGA ATGACTTGGC CATCAAGTTC CTGAACCAGG ACTATGAGGA GCTGAGAAGG 
GAGTGTGTGG AGAGTGGCAG GCTGTTTGAG GACCCTTGCT TTCCTGCAGT GCCCCAATCA 
CTGGGCTTCA AGGAGCTCGC GCCAAACTCC TCCAAAACAC GTGGTGTGAA GTGGATAAGA 
CCCACTGAGC TGTCAGAGAA TCCTCAGTTT ATAGTGGGCG GAGCCACGCG TACAGATATT 
TGCCAGGGGG CCCTAGGTGA CTGTTGGCTT TTGGCTGCCA TAGCGTCGTT GACATTAAAC 
GACAAGCTGC TTCACAGAGT TGTACCACAT GGGCAAAGCT TTACAAATGA GTATGCTGGA 
ATCTTCCACT TCCAGTTCTG GCAGTTTGGC GAGTGGGTAG ACATTGTAAT CGATGACCGC 
TTGCCTGTGA AGGATAAAGA GCTGATGTTT GTTCACTCAG CAGAGGGAAA TGAGTTCTGG 
AGCGCCCTGC TGGAGAAGGC ATATGCTAAG CTAAATGGCT CATATGAAGC TCTGTCCGGA 
GGTTCCACCA CCGAAGGCTT TGAGGACTTT ACTGGCGGAG TTGCCGAAAT GTATGAACTG 
CGCAGCGCTC CCAAAGACCT GCACCGCATC ATTGCCAAAG CCCTGGAGAG AGGCTCACTG 
CTGGGCTGCT CCATTGATAT CACCAGTGCC TTTGACATGG AGGCTGTGAC CTTTAAGAAG 
CTAGTGAAGG GCCATGCTTA CTCCGTCACA GCTCTCAGAG AGGTGAATTT CCGTGGCAAC 
AGAGAGAGAT TGATCCGTAT CCGAAACCCT TGGGGACAAG TTGAATGGAC TGGGGCTTGG 
AGCGACAATT CGTCCGAGTG GAATGGGATT GATCCATCGG AAAGAGAGGA ACTGAACAAC 
CACATGGAGG ATGGCGAGTT TTGGATGTCA TTTCAGGAGT TCAAACGTCA GTTTTCTCGT 
CTGGAGATCT GCAACCTCAC TCCTGATGCT CTGAGTGATG ATTCCCAACA TTTCTGGAAC 
ACAATTCAGT TTAACGGCAC TTGGAGGAAA GGAAGTACAG CAGGAGGCTG CAGGAACAAT 
CCCAACACAT TCTGGATAAA CCCTCAATAT AAGATCACAC TACTGGAGGA AGATGATGAT 
CCTGAGGACA ATGAGGTGGC CTGCACCTTC ATGGTGGCTT TGATGCAGAA GGACAGACGG 
CGTTTCCGCA AGCAGGGACA GGACATGCAC ACCATCGGCT TCGCCTTATA TGAGGTCCCA 
GATGAGTTTC TTGGCTCCCA GAATGTTCAC CTGAAGAAGG ATTTTTTCTT GCGTCACTCG 
TCCTGTGCAC GCTCCGAGAC CTTTATTAAT CTGCGAGAGG TGAGCACGCG ACTGAGGCTG 
CCACCGGGCG AGTATCTCAT TGTCCCTTCC ACTTTTGAGC CTGGAAAAGA GGCTGACTTC 
GTCCTGCGAG TATTCACTGA GAAACAGTCA GAAACTCAAG AAATGGATGA TGAAATTTCA 
TTTAACCTGG AAGATGAAGA GGCAGTCTCA GAGGAGAACA TTGATGCCTC GTTTAAAAAG 
ATGTTTGCTC AGCTGTCTGG AGAGGACATG GAGATTTCAG TTCACGAGCT CCGGACCATT 
CTGAACCGAG TGATGACAAA ACACCGAGAC TTAAAGACAG ATGGGTTCAG TTTGGAGTCT 
TGTAGGTGCA TGATCAACCT GATGGACAAA GACGGCAGTG CACGACTGGG AATTGTGGAG 
TTCCAGATTC TGTGGAATAA AATCAGGAAG TGGCTGGGTG TCTTCAGACA GTTCGATCTG 
GATAAATCTG GAACTATGAG CTCCTACGAA ATGCGCCTGG CTGTGGAGTC TGCAGGTTTT 
AAACTCAATA ACAAGCTACA TCAGATCCTG GTGGCCCGAT ATTCAGACGG AGATGTGATT 
GACTTCGACA ACTTTGTCTG CTGCCTGGTT AAACTGGAGG CCATGTTCAA GTCTTTCAAA 
GAGCTGGAGA AAGAGGGCTC AGGTGTCGCA GAGCTCAATA TTAGCGAGTG GCTGTATTTG 
ACCATGTGCG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

capn1b ( XM_005174031.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005174088.1 Danio rerio calpain 1, (mu/I) large subunit b (capn1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa27025
Clone ID Related Accession (Same CDS sequence)	XM_009296390.2 , XM_005174031.3 , XM_005174031.4
Accession Version	XM_005174031.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	calpain 1, large subunit isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005174031.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTCAA TTGGAGGTGT GTCGACGCGC ATCTACAAGG AGCGCCTGCA GGCAGAAGGG 
ATGGGAGCGA ATGACTTGGC CATCAAGTTC CTGAACCAGG ACTATGAGGA GCTGAGAAGG 
GAGTGTGTGG AGAGTGGCAG GCTGTTTGAG GACCCTTGCT TTCCTGCAGT GCCCCAATCA 
CTGGGCTTCA AGGAGCTCGC GCCAAACTCC TCCAAAACAC GTGGTGTGAA GTGGATAAGA 
CCCACTGAGC TGTCAGAGAA TCCTCAGTTT ATAGTGGGCG GAGCCACGCG TACAGATATT 
TGCCAGGGGG CCCTAGGTGA CTGTTGGCTT TTGGCTGCCA TAGCGTCGTT GACATTAAAC 
GACAAGCTGC TTCACAGAGT TGTACCACAT GGGCAAAGCT TTACAAATGA GTATGCTGGA 
ATCTTCCACT TCCAGTTCTG GCAGTTTGGC GAGTGGGTAG ACATTGTAAT CGATGACCGC 
TTGCCTGTGA AGGATAAAGA GCTGATGTTT GTTCACTCAG CAGAGGGAAA TGAGTTCTGG 
AGCGCCCTGC TGGAGAAGGC ATATGCTAAG CTAAATGGCT CATATGAAGC TCTGTCCGGA 
GGTTCCACCA CCGAAGGCTT TGAGGACTTT ACTGGCGGAG TTGCCGAAAT GTATGAACTG 
CGCAGCGCTC CCAAAGACCT GCACCGCATC ATTGCCAAAG CCCTGGAGAG AGGCTCACTG 
CTGGGCTGCT CCATTGATAT CACCAGTGCC TTTGACATGG AGGCTGTGAC CTTTAAGAAG 
CTAGTGAAGG GCCATGCTTA CTCCGTCACA GCTCTCAGAG AGGTGAATTT CCGTGGCAAC 
AGAGAGAGAT TGATCCGTAT CCGAAACCCT TGGGGACAAG TTGAATGGAC TGGGGCTTGG 
AGCGACAATT CGTCCGAGTG GAATGGGATT GATCCATCGG AAAGAGAGGA ACTGAACAAC 
CACATGGAGG ATGGCGAGTT TTGGATGTCA TTTCAGGAGT TCAAACGTCA GTTTTCTCGT 
CTGGAGATCT GCAACCTCAC TCCTGATGCT CTGAGTGATG ATTCCCAACA TTTCTGGAAC 
ACAATTCAGT TTAACGGCAC TTGGAGGAAA GGAAGTACAG CAGGAGGCTG CAGGAACAAT 
CCCAACACAT TCTGGATAAA CCCTCAATAT AAGATCACAC TACTGGAGGA AGATGATGAT 
CCTGAGGACA ATGAGGTGGC CTGCACCTTC ATGGTGGCTT TGATGCAGAA GGACAGACGG 
CGTTTCCGCA AGCAGGGACA GGACATGCAC ACCATCGGCT TCGCCTTATA TGAGGTCCCA 
GATGAGTTTC TTGGCTCCCA GAATGTTCAC CTGAAGAAGG ATTTTTTCTT GCGTCACTCG 
TCCTGTGCAC GCTCCGAGAC CTTTATTAAT CTGCGAGAGG TGAGCACGCG ACTGAGGCTG 
CCACCGGGCG AGTATCTCAT TGTCCCTTCC ACTTTTGAGC CTGGAAAAGA GGCTGACTTC 
GTCCTGCGAG TATTCACTGA GAAACAGTCA GAAACTCAAG AAATGGATGA TGAAATTTCA 
TTTAACCTGG AAGATGAAGA GGCAGTCTCA GAGGAGAACA TTGATGCCTC GTTTAAAAAG 
ATGTTTGCTC AGCTGTCTGG AGAGGACATG GAGATTTCAG TTCACGAGCT CCGGACCATT 
CTGAACCGAG TGATGACAAA ACACCGAGAC TTAAAGACAG ATGGGTTCAG TTTGGAGTCT 
TGTAGGTGCA TGATCAACCT GATGGACAAA GACGGCAGTG CACGACTGGG AATTGTGGAG 
TTCCAGATTC TGTGGAATAA AATCAGGAAG TGGCTGGGTG TCTTCAGACA GTTCGATCTG 
GATAAATCTG GAACTATGAG CTCCTACGAA ATGCGCCTGG CTGTGGAGTC TGCAGGTTTT 
AAACTCAATA ACAAGCTACA TCAGATCCTG GTGGCCCGAT ATTCAGACGG AGATGTGATT 
GACTTCGACA ACTTTGTCTG CTGCCTGGTT AAACTGGAGG CCATGTTCAA GTCTTTCAAA 
GAGCTGGAGA AAGAGGGCTC AGGTGTCGCA GAGCTCAATA TTAGCGAGTG GCTGTATTTG 
ACCATGTGCG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005174088.1
CDS	225..2339
Translation	MFSIGGVSTRIYKERLQAEGMGANDLAIKFLNQDYEELRRECVESGRLFEDPCFPAVPQSLGFKELAPNSSKTRGVKWIRPTELSENPQFIVGGATRTDICQGALGDCWLLAAIASLTLNDKLLHRVVPHGQSFTNEYAGIFHFQFWQFGEWVDIVIDDRLPVKDKELMFVHSAEGNEFWSALLEKAYAKLNGSYEALSGGSTTEGFEDFTGGVAEMYELRSAPKDLHRIIAKALERGSLLGCSIDITSAFDMEAVTFKKLVKGHAYSVTALREVNFRGNRERLIRIRNPWGQVEWTGAWSDNSSEWNGIDPSEREELNNHMEDGEFWMSFQEFKRQFSRLEICNLTPDALSDDSQHFWNTIQFNGTWRKGSTAGGCRNNPNTFWINPQYKITLLEEDDDPEDNEVACTFMVALMQKDRRRFRKQGQDMHTIGFALYEVPDEFLGSQNVHLKKDFFLRHSSCARSETFINLREVSTRLRLPPGEYLIVPSTFEPGKEADFVLRVFTEKQSETQEMDDEISFNLEDEEAVSEENIDASFKKMFAQLSGEDMEISVHELRTILNRVMTKHRDLKTDGFSLESCRCMINLMDKDGSARLGIVEFQILWNKIRKWLGVFRQFDLDKSGTMSSYEMRLAVESAGFKLNNKLHQILVARYSDGDVIDFDNFVCCLVKLEAMFKSFKELEKEGSGVAELNISEWLYLTMCG

Target ORF information:

RefSeq Version	XM_005174031.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calpain 1, (mu/I) large subunit b (capn1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005174031.3

ORF Insert Sequence:

ATGTTTTCAA TTGGAGGTGT GTCGACGCGC ATCTACAAGG AGCGCCTGCA GGCAGAAGGG 
ATGGGAGCGA ATGACTTGGC CATCAAGTTC CTGAACCAGG ACTATGAGGA GCTGAGAAGG 
GAGTGTGTGG AGAGTGGCAG GCTGTTTGAG GACCCTTGCT TTCCTGCAGT GCCCCAATCA 
CTGGGCTTCA AGGAGCTCGC GCCAAACTCC TCCAAAACAC GTGGTGTGAA GTGGATAAGA 
CCCACTGAGC TGTCAGAGAA TCCTCAGTTT ATAGTGGGCG GAGCCACGCG TACAGATATT 
TGCCAGGGGG CCCTAGGTGA CTGTTGGCTT TTGGCTGCCA TAGCGTCGTT GACATTAAAC 
GACAAGCTGC TTCACAGAGT TGTACCACAT GGGCAAAGCT TTACAAATGA GTATGCTGGA 
ATCTTCCACT TCCAGTTCTG GCAGTTTGGC GAGTGGGTAG ACATTGTAAT CGATGACCGC 
TTGCCTGTGA AGGATAAAGA GCTGATGTTT GTTCACTCAG CAGAGGGAAA TGAGTTCTGG 
AGCGCCCTGC TGGAGAAGGC ATATGCTAAG CTAAATGGCT CATATGAAGC TCTGTCCGGA 
GGTTCCACCA CCGAAGGCTT TGAGGACTTT ACTGGCGGAG TTGCCGAAAT GTATGAACTG 
CGCAGCGCTC CCAAAGACCT GCACCGCATC ATTGCCAAAG CCCTGGAGAG AGGCTCACTG 
CTGGGCTGCT CCATTGATAT CACCAGTGCC TTTGACATGG AGGCTGTGAC CTTTAAGAAG 
CTAGTGAAGG GCCATGCTTA CTCCGTCACA GCTCTCAGAG AGGTGAATTT CCGTGGCAAC 
AGAGAGAGAT TGATCCGTAT CCGAAACCCT TGGGGACAAG TTGAATGGAC TGGGGCTTGG 
AGCGACAATT CGTCCGAGTG GAATGGGATT GATCCATCGG AAAGAGAGGA ACTGAACAAC 
CACATGGAGG ATGGCGAGTT TTGGATGTCA TTTCAGGAGT TCAAACGTCA GTTTTCTCGT 
CTGGAGATCT GCAACCTCAC TCCTGATGCT CTGAGTGATG ATTCCCAACA TTTCTGGAAC 
ACAATTCAGT TTAACGGCAC TTGGAGGAAA GGAAGTACAG CAGGAGGCTG CAGGAACAAT 
CCCAACACAT TCTGGATAAA CCCTCAATAT AAGATCACAC TACTGGAGGA AGATGATGAT 
CCTGAGGACA ATGAGGTGGC CTGCACCTTC ATGGTGGCTT TGATGCAGAA GGACAGACGG 
CGTTTCCGCA AGCAGGGACA GGACATGCAC ACCATCGGCT TCGCCTTATA TGAGGTCCCA 
GATGAGTTTC TTGGCTCCCA GAATGTTCAC CTGAAGAAGG ATTTTTTCTT GCGTCACTCG 
TCCTGTGCAC GCTCCGAGAC CTTTATTAAT CTGCGAGAGG TGAGCACGCG ACTGAGGCTG 
CCACCGGGCG AGTATCTCAT TGTCCCTTCC ACTTTTGAGC CTGGAAAAGA GGCTGACTTC 
GTCCTGCGAG TATTCACTGA GAAACAGTCA GAAACTCAAG AAATGGATGA TGAAATTTCA 
TTTAACCTGG AAGATGAAGA GGCAGTCTCA GAGGAGAACA TTGATGCCTC GTTTAAAAAG 
ATGTTTGCTC AGCTGTCTGG AGAGGACATG GAGATTTCAG TTCACGAGCT CCGGACCATT 
CTGAACCGAG TGATGACAAA ACACCGAGAC TTAAAGACAG ATGGGTTCAG TTTGGAGTCT 
TGTAGGTGCA TGATCAACCT GATGGACAAA GACGGCAGTG CACGACTGGG AATTGTGGAG 
TTCCAGATTC TGTGGAATAA AATCAGGAAG TGGCTGGGTG TCTTCAGACA GTTCGATCTG 
GATAAATCTG GAACTATGAG CTCCTACGAA ATGCGCCTGG CTGTGGAGTC TGCAGGTTTT 
AAACTCAATA ACAAGCTACA TCAGATCCTG GTGGCCCGAT ATTCAGACGG AGATGTGATT 
GACTTCGACA ACTTTGTCTG CTGCCTGGTT AAACTGGAGG CCATGTTCAA GTCTTTCAAA 
GAGCTGGAGA AAGAGGGCTC AGGTGTCGCA GAGCTCAATA TTAGCGAGTG GCTGTATTTG 
ACCATGTGCG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

capn1b ( XM_005174031.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005174088.1 Danio rerio calpain 1, (mu/I) large subunit b (capn1b), transcript variant X1, mRNA.

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CloneID	ODa27025
Clone ID Related Accession (Same CDS sequence)	XM_009296390.2 , XM_005174031.3 , XM_005174031.4
Accession Version	XM_005174031.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calpain 1, large subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005174031.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTCAA TTGGAGGTGT GTCGACGCGC ATCTACAAGG AGCGCCTGCA GGCAGAAGGG 
ATGGGAGCGA ATGACTTGGC CATCAAGTTC CTGAACCAGG ACTATGAGGA GCTGAGAAGG 
GAGTGTGTGG AGAGTGGCAG GCTGTTTGAG GACCCTTGCT TTCCTGCAGT GCCCCAATCA 
CTGGGCTTCA AGGAGCTCGC GCCAAACTCC TCCAAAACAC GTGGTGTGAA GTGGATAAGA 
CCCACTGAGC TGTCAGAGAA TCCTCAGTTT ATAGTGGGCG GAGCCACGCG TACAGATATT 
TGCCAGGGGG CCCTAGGTGA CTGTTGGCTT TTGGCTGCCA TAGCGTCGTT GACATTAAAC 
GACAAGCTGC TTCACAGAGT TGTACCACAT GGGCAAAGCT TTACAAATGA GTATGCTGGA 
ATCTTCCACT TCCAGTTCTG GCAGTTTGGC GAGTGGGTAG ACATTGTAAT CGATGACCGC 
TTGCCTGTGA AGGATAAAGA GCTGATGTTT GTTCACTCAG CAGAGGGAAA TGAGTTCTGG 
AGCGCCCTGC TGGAGAAGGC ATATGCTAAG CTAAATGGCT CATATGAAGC TCTGTCCGGA 
GGTTCCACCA CCGAAGGCTT TGAGGACTTT ACTGGCGGAG TTGCCGAAAT GTATGAACTG 
CGCAGCGCTC CCAAAGACCT GCACCGCATC ATTGCCAAAG CCCTGGAGAG AGGCTCACTG 
CTGGGCTGCT CCATTGATAT CACCAGTGCC TTTGACATGG AGGCTGTGAC CTTTAAGAAG 
CTAGTGAAGG GCCATGCTTA CTCCGTCACA GCTCTCAGAG AGGTGAATTT CCGTGGCAAC 
AGAGAGAGAT TGATCCGTAT CCGAAACCCT TGGGGACAAG TTGAATGGAC TGGGGCTTGG 
AGCGACAATT CGTCCGAGTG GAATGGGATT GATCCATCGG AAAGAGAGGA ACTGAACAAC 
CACATGGAGG ATGGCGAGTT TTGGATGTCA TTTCAGGAGT TCAAACGTCA GTTTTCTCGT 
CTGGAGATCT GCAACCTCAC TCCTGATGCT CTGAGTGATG ATTCCCAACA TTTCTGGAAC 
ACAATTCAGT TTAACGGCAC TTGGAGGAAA GGAAGTACAG CAGGAGGCTG CAGGAACAAT 
CCCAACACAT TCTGGATAAA CCCTCAATAT AAGATCACAC TACTGGAGGA AGATGATGAT 
CCTGAGGACA ATGAGGTGGC CTGCACCTTC ATGGTGGCTT TGATGCAGAA GGACAGACGG 
CGTTTCCGCA AGCAGGGACA GGACATGCAC ACCATCGGCT TCGCCTTATA TGAGGTCCCA 
GATGAGTTTC TTGGCTCCCA GAATGTTCAC CTGAAGAAGG ATTTTTTCTT GCGTCACTCG 
TCCTGTGCAC GCTCCGAGAC CTTTATTAAT CTGCGAGAGG TGAGCACGCG ACTGAGGCTG 
CCACCGGGCG AGTATCTCAT TGTCCCTTCC ACTTTTGAGC CTGGAAAAGA GGCTGACTTC 
GTCCTGCGAG TATTCACTGA GAAACAGTCA GAAACTCAAG AAATGGATGA TGAAATTTCA 
TTTAACCTGG AAGATGAAGA GGCAGTCTCA GAGGAGAACA TTGATGCCTC GTTTAAAAAG 
ATGTTTGCTC AGCTGTCTGG AGAGGACATG GAGATTTCAG TTCACGAGCT CCGGACCATT 
CTGAACCGAG TGATGACAAA ACACCGAGAC TTAAAGACAG ATGGGTTCAG TTTGGAGTCT 
TGTAGGTGCA TGATCAACCT GATGGACAAA GACGGCAGTG CACGACTGGG AATTGTGGAG 
TTCCAGATTC TGTGGAATAA AATCAGGAAG TGGCTGGGTG TCTTCAGACA GTTCGATCTG 
GATAAATCTG GAACTATGAG CTCCTACGAA ATGCGCCTGG CTGTGGAGTC TGCAGGTTTT 
AAACTCAATA ACAAGCTACA TCAGATCCTG GTGGCCCGAT ATTCAGACGG AGATGTGATT 
GACTTCGACA ACTTTGTCTG CTGCCTGGTT AAACTGGAGG CCATGTTCAA GTCTTTCAAA 
GAGCTGGAGA AAGAGGGCTC AGGTGTCGCA GAGCTCAATA TTAGCGAGTG GCTGTATTTG 
ACCATGTGCG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005174088.1
CDS	173..2287
Translation	MFSIGGVSTRIYKERLQAEGMGANDLAIKFLNQDYEELRRECVESGRLFEDPCFPAVPQSLGFKELAPNSSKTRGVKWIRPTELSENPQFIVGGATRTDICQGALGDCWLLAAIASLTLNDKLLHRVVPHGQSFTNEYAGIFHFQFWQFGEWVDIVIDDRLPVKDKELMFVHSAEGNEFWSALLEKAYAKLNGSYEALSGGSTTEGFEDFTGGVAEMYELRSAPKDLHRIIAKALERGSLLGCSIDITSAFDMEAVTFKKLVKGHAYSVTALREVNFRGNRERLIRIRNPWGQVEWTGAWSDNSSEWNGIDPSEREELNNHMEDGEFWMSFQEFKRQFSRLEICNLTPDALSDDSQHFWNTIQFNGTWRKGSTAGGCRNNPNTFWINPQYKITLLEEDDDPEDNEVACTFMVALMQKDRRRFRKQGQDMHTIGFALYEVPDEFLGSQNVHLKKDFFLRHSSCARSETFINLREVSTRLRLPPGEYLIVPSTFEPGKEADFVLRVFTEKQSETQEMDDEISFNLEDEEAVSEENIDASFKKMFAQLSGEDMEISVHELRTILNRVMTKHRDLKTDGFSLESCRCMINLMDKDGSARLGIVEFQILWNKIRKWLGVFRQFDLDKSGTMSSYEMRLAVESAGFKLNNKLHQILVARYSDGDVIDFDNFVCCLVKLEAMFKSFKELEKEGSGVAELNISEWLYLTMCG

Target ORF information:

RefSeq Version	XM_005174031.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpain 1, (mu/I) large subunit b (capn1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005174031.4

ORF Insert Sequence:

ATGTTTTCAA TTGGAGGTGT GTCGACGCGC ATCTACAAGG AGCGCCTGCA GGCAGAAGGG 
ATGGGAGCGA ATGACTTGGC CATCAAGTTC CTGAACCAGG ACTATGAGGA GCTGAGAAGG 
GAGTGTGTGG AGAGTGGCAG GCTGTTTGAG GACCCTTGCT TTCCTGCAGT GCCCCAATCA 
CTGGGCTTCA AGGAGCTCGC GCCAAACTCC TCCAAAACAC GTGGTGTGAA GTGGATAAGA 
CCCACTGAGC TGTCAGAGAA TCCTCAGTTT ATAGTGGGCG GAGCCACGCG TACAGATATT 
TGCCAGGGGG CCCTAGGTGA CTGTTGGCTT TTGGCTGCCA TAGCGTCGTT GACATTAAAC 
GACAAGCTGC TTCACAGAGT TGTACCACAT GGGCAAAGCT TTACAAATGA GTATGCTGGA 
ATCTTCCACT TCCAGTTCTG GCAGTTTGGC GAGTGGGTAG ACATTGTAAT CGATGACCGC 
TTGCCTGTGA AGGATAAAGA GCTGATGTTT GTTCACTCAG CAGAGGGAAA TGAGTTCTGG 
AGCGCCCTGC TGGAGAAGGC ATATGCTAAG CTAAATGGCT CATATGAAGC TCTGTCCGGA 
GGTTCCACCA CCGAAGGCTT TGAGGACTTT ACTGGCGGAG TTGCCGAAAT GTATGAACTG 
CGCAGCGCTC CCAAAGACCT GCACCGCATC ATTGCCAAAG CCCTGGAGAG AGGCTCACTG 
CTGGGCTGCT CCATTGATAT CACCAGTGCC TTTGACATGG AGGCTGTGAC CTTTAAGAAG 
CTAGTGAAGG GCCATGCTTA CTCCGTCACA GCTCTCAGAG AGGTGAATTT CCGTGGCAAC 
AGAGAGAGAT TGATCCGTAT CCGAAACCCT TGGGGACAAG TTGAATGGAC TGGGGCTTGG 
AGCGACAATT CGTCCGAGTG GAATGGGATT GATCCATCGG AAAGAGAGGA ACTGAACAAC 
CACATGGAGG ATGGCGAGTT TTGGATGTCA TTTCAGGAGT TCAAACGTCA GTTTTCTCGT 
CTGGAGATCT GCAACCTCAC TCCTGATGCT CTGAGTGATG ATTCCCAACA TTTCTGGAAC 
ACAATTCAGT TTAACGGCAC TTGGAGGAAA GGAAGTACAG CAGGAGGCTG CAGGAACAAT 
CCCAACACAT TCTGGATAAA CCCTCAATAT AAGATCACAC TACTGGAGGA AGATGATGAT 
CCTGAGGACA ATGAGGTGGC CTGCACCTTC ATGGTGGCTT TGATGCAGAA GGACAGACGG 
CGTTTCCGCA AGCAGGGACA GGACATGCAC ACCATCGGCT TCGCCTTATA TGAGGTCCCA 
GATGAGTTTC TTGGCTCCCA GAATGTTCAC CTGAAGAAGG ATTTTTTCTT GCGTCACTCG 
TCCTGTGCAC GCTCCGAGAC CTTTATTAAT CTGCGAGAGG TGAGCACGCG ACTGAGGCTG 
CCACCGGGCG AGTATCTCAT TGTCCCTTCC ACTTTTGAGC CTGGAAAAGA GGCTGACTTC 
GTCCTGCGAG TATTCACTGA GAAACAGTCA GAAACTCAAG AAATGGATGA TGAAATTTCA 
TTTAACCTGG AAGATGAAGA GGCAGTCTCA GAGGAGAACA TTGATGCCTC GTTTAAAAAG 
ATGTTTGCTC AGCTGTCTGG AGAGGACATG GAGATTTCAG TTCACGAGCT CCGGACCATT 
CTGAACCGAG TGATGACAAA ACACCGAGAC TTAAAGACAG ATGGGTTCAG TTTGGAGTCT 
TGTAGGTGCA TGATCAACCT GATGGACAAA GACGGCAGTG CACGACTGGG AATTGTGGAG 
TTCCAGATTC TGTGGAATAA AATCAGGAAG TGGCTGGGTG TCTTCAGACA GTTCGATCTG 
GATAAATCTG GAACTATGAG CTCCTACGAA ATGCGCCTGG CTGTGGAGTC TGCAGGTTTT 
AAACTCAATA ACAAGCTACA TCAGATCCTG GTGGCCCGAT ATTCAGACGG AGATGTGATT 
GACTTCGACA ACTTTGTCTG CTGCCTGGTT AAACTGGAGG CCATGTTCAA GTCTTTCAAA 
GAGCTGGAGA AAGAGGGCTC AGGTGTCGCA GAGCTCAATA TTAGCGAGTG GCTGTATTTG 
ACCATGTGCG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Mutations in CAPN1 Cause Autosomal-Recessive Hereditary Spastic Paraplegia.
American journal of human genetics98(5)1038-1046(2016 May)
Gan-Or Z,Bouslam N,Birouk N,Lissouba A,Chambers DB,V?ri?pe J,Androschuk A,Laurent SB,Rochefort D,Spiegelman D,Dionne-Laporte A,Szuto A,Liao M,Figlewicz DA,Bouhouche A,Benomar A,Yahyaoui M,Ouazzani R,Yoon G,Dupr? N,Suchowersky O,Bolduc FV,Parker JA,Dion PA,Drapeau P,Rouleau GA,Ouled Amar Bencheikh B

Phenotype anchoring in zebrafish reveals a potential role for matrix metalloproteinases (MMPs) in tamoxifen's effects on skin epithelium.
Toxicology and applied pharmacology29631-41(2016 Apr)
Bugel SM,Wehmas LC,La Du JK,Tanguay RL

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capn1b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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