col18a1a cDNA ORF clone, Danio rerio(zebrafish)

The following col18a1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the col18a1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa98452 NM_001349195.1
Latest version!
Danio rerio collagen type XVIII alpha 1 chain a (col18a1a), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
ODa143962 XM_021478720.1
Latest version!
Danio rerio collagen type XVIII alpha 1 chain a (col18a1a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa143963 XM_021478721.1
Latest version!
Danio rerio collagen type XVIII alpha 1 chain a (col18a1a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
ODa143964 XM_021478722.1
Latest version!
Danio rerio collagen type XVIII alpha 1 chain a (col18a1a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa98452
    Clone ID Related Accession (Same CDS sequence) NM_001349195.1
    Accession Version NM_001349195.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4152bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-23
    Organism Danio rerio(zebrafish)
    Product collagen type XVIII, alpha 1 precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX927363.9 and CT030212.9. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC163779.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGATGGCTG AACTCAGGTT TTGGCTGTGC CTCTTTATTT TGGCATGCGT GAGGATACGC 
    CACACACATG GCTGGTTCTG GTTTGATGAC TCCAAGGAAA TTGAGAAGGG CGTGCAGACA
    CCAGCATACC TGACCACCGT AGGGCCCACA TCCCTGCCGA GGACAGAACC ATCCAGAACA
    ACAGAAGAAA GTGGAGTGTC GCTGTTGCAA CTAATTGGCG ATCCTCCACC CAACGGCGTC
    TCCAAAGTCT TCGACCAAGC AAACAGTCCT GGATATGTGT TTGGACCAAG CTCAAATGTG
    GGTCAGTCTG CTGCAGCCCA CCTGCCCAAT CCCTTCTACC GCGATTTCTC CCTCATCTTC
    AATATAAAAC CCACATCCTC CAAGCCTGGC GTGATCTTCT CCATCACTGA CCCCACTCAG
    AACATCATGT ATGTTGGCGT GAAGCTGTCG GCGGTGGAAA GAGGCAAACA GTCCATTATC
    TTCTACTACA CCGAGCCTGA TTCGCAGAGC TCCTATGAGG CGGCCAGATT CTCTGTTTCC
    TCCATGTTGA ACATATGGAC GCGCTTTTCC ATCAGCGTTT TGAACGAGCA GGTTTCTCTC
    TACTTTAACT GCGACTCGGA TCCTCAGGTT GTCAATTTCG AGCGCTCGCC TGATGATATG
    GACATGGATG CTGGCGCTGG AGTGTTTGTG GGACATGCCA GTGGAGCAGA CCCGGATAAG
    TTTTTGGGTG TTATTGGTGA TCTGAGGGTG TCGAGAGACC CTGGGGCGGC TGAACGGCAC
    TGTGAAGAAG AGGAGGATGA CTATGCAACA GAGGCATCAG GTGATTACGG AGCAAGTGGA
    GATGGAGAGC AGCCATCACC AGTTCAGCCA ACACCTCCAT CATCTCGGCC AATCCAGCGG
    CCACCGGTGA TTAGCAGGGA TCCTATACAG CGGCCACCGG TGATTAGCAG GGAGCCTATA
    CAGCAGACAG TTGCAAGAGG AGAGAAGGGT GACAGAGGGG AGAAGGGTGC TAAAGGTGAC
    AGGGGTCTTG CTGGGCCAAA GGGTGATGTG GGGTCTGGAT CAGTGTCTGG TGGAGGATCA
    AAAGGAGACA AGGGCGTCCC AGGAGAGAAA GGAATGAAGG GCACTTCTGG TTTTGGTTAT
    CCTGGATCTA AGGGTGACCG TGGCCCCCCT GGACCTCCAG GACCTCCGGG TCCCCAAGGG
    CCCAGTGCAG AGGTAGAGGT GCGGGGCGAT GGGTCAGTTG TGCAGAAAGT CACAGGACCA
    AGAGGACCAC CTGGACCCCA AGGACCCCCT GGTCCCCCTG GACCTGAAGG GGAGCCGGGT
    GACCCTGGTG AAGATGGAAA AGCTGGCCAA GTTGGACCTT CTGGTTTTCC TGGAAATCCT
    GGGAATCCTG GACCCAAAGG AGATAAGGGA GATCGAGGTG AAAGTCAGCC AGGACCCCGT
    GGGCCGCCTG GTCCTCCCGG CCCACCAGGA CCCTCCTCTG GCTTTGATAG ACCTACGTTT
    GTGGACATGG AGGGCTCTGG ATTTGATATG GACAGTGTGC GGGCAGTGCC TGGTTTGCCT
    GGTCCACCTG GTCCTCCTGG TCCCCCTGGT CCCCCTGGCT CTGCATCTTC AGGCTCTGGT
    GGTTTTGGGC CACCTGGTCC TCCTGGGCAA AATGGGGCGC CAGGTCAGCC GGGACTTTCG
    GGTGTACCAG GTGCTGATGG AAAACCAGGC TTACCTGGTC CAAAAGGAGA GAAGGGTGAT
    GCCGGTGAGC TGGGCCTTCC TGGACCGGTG GGAGAGAAGG GTGCCAAAGG TTCTTCTGGT
    CCCCCTGGCA CCACTGGGAT TGGTGGGCCT GCTGGTCTTC CAGGACCAAT TGGACCTATT
    GGACTTCCAG GACCACCTGG TCCTCCTGGA CCAAGCTTTA ATGTTGGTTT TGATGACATG
    GAAGGTTCTG GTGTTCACTT CAGTTCAGTA CCTGGAGTAA GAGGACCAGT TGGAATCCCG
    GGGCCACCTG GTGTCCCAGG GCCACAGGGT CAGCCTGGAT TCCCAGGGCT TCCTGGTGAG
    AAAGGCAGTT TAGGAGCTCA GGGAAATGAT GGTCTTCCTG GTGCAGATGG CTTCCCTGGA
    CCACAGGGAC CAAAGGGCGA CAAAGGAGAG CGAGGCGACA GGGGTGAACC TGGTCGAGAT
    GGACATGGTC ATCCAGGCCA ACCAGGCCCT CCTGGACCAC CTGGACAGAT TATTTATCGC
    TACTCTGAAC ATTATGATGA AGTTGGAGGA ACGGGGGCTC AGGGTGGTTC TGGTGTTCCT
    GGCCAAGCAG GATTCCCAGG CCCAATGGGA CCAAAGGGTG ACAGAGGTGA GCCTGGTCTT
    CCAGGCTATG ACATTAAGGG GGAGAAAGGA GAACCTGGAC TTATTCTTGG ACCTGATGGA
    AATCCACTTT ACCTTGGTGG ATTGACAGGA CAGAAGGGTG AGAGAGGTAT TTCTGGACCA
    GTCGGACCTC CTGGTCCAGC AGGACCTCCT GGTGCAAAAG GTGAATTCGG AATGCCAGGC
    AGACCTGGTC GTCCAGGTGT GAACGGATAT AAAGGAGAGA AGGGAGAGCC AAGTTCAGGC
    TCTGGATATG GTTACCCGGG TCCCCCTGGT CCCCCAGGAC CCCCTGGTCC CCCCGGACCC
    GCTGTGCCCT TGGACAGATT TGGCAGATAT GAAGATTATG CAAGGCTCTA TCCAGCTACG
    AAAGGAGAGA AAGGAGACCA AGGAGCTCCT GGCATTCCCG GATCGCCAGG TTTTTCCACA
    AACTTTGATA TATATTCACT CAAGAATGAG ATGAAGGGTG AGCGGGGAGA GTCTGGTCTT
    AAAGGAGAGA AAGGAGAACC AGGAGGTGGA TTTTATGACC CTCGTTTTGG AGCTGTACAA
    GGACCACCAG GGAACCCAGG ACCTGCTGGA CCTAAAGGAG ACTCAATCAG AGGCCCACCT
    GGACCCCAGG GCCCACCAGG AACACCCGGG GTTGGTTATG ATGGTCGACC AGGTAATCCA
    GGACCTCCTG GACCACCTGG TCCACCAGGG TCCCCATCAC TGCCAGGACC CTATAGGCCA
    CCACTCAGTA TTCCTGGACC TCCAGGTCCT CCTGGCCCAC CTGGAATTCC TGGCACTGGA
    TCAGGGGTGA CTGTCCTGAG GTCTCATGAC ATAATGATTG CTACAGCACG CAGACAGACA
    GAAGGGACTC TGATTTACAT TCTGGACAGA AACGATCTCT ACCTCAGAGT TCGGGACGGT
    GTTAGACAAG TGATGCTTGG GGATTATAAA CCTCTTTATG GAGAGCTGGA CAATGAAGTG
    GCAGCAGTCC AGCCACCCCC TGTTGTTCAC TATTCCCAGG ACCACACGGC TAACAATGGC
    GCAGAGCAGA TTTCTCCACC ACACCAGCCT ATTGATTTCC CAACGAGAGA GCAGGAGAAC
    AGAAACCCCA ACCCGCAACC TGACCCCCGA TACCCGGATC CACGATACCC ACCATACACT
    GACAACAGAT ACACCGATCC TGTAGAGCCC AACATCAGAT ATCCTGTTCA ACCCGAGAGG
    AATCCAATCA CTCCAGCCCG CCGTCCTATC CCTCCTGTCA ATCAGCCAGG AGGCCACGCC
    CACACCCACA CTTCCGGACC AGGGTTGCAT CTTATTGCTC TGAATTCTCC ACAAGTGGGC
    AACATGAGAG GCATTCGCGG GGCAGACTTC ATGTGCTTCC AGCAGGCACG GGCCGTTGGC
    ATGAAGGGAA CGTTCAGAGC CTTCCTGTCC TCCAAACTCC AGGATCTCTA CAGTATCGTC
    CGCAGATCTG ACAGAGAGAC ATTACCAATT GTTAACCTAA AGGATCAAGT CCTCTTCAGC
    AGCTGGGAGT CTCTCTTCAG TGACTCAGAG AGCAGGATGA AGGACAATGC ACCCATCTAC
    TCGTTTGATG GCAGAGATGT CCTGAGGGAC AGTGCCTGGC CAGAGAAGAT GATTTGGCAC
    GGATCGGATG GCAGGGGTCA CAGGCAAACG GATAACTACT GCGAGACATG GAGGGCCGGA
    GACCGCGCTG TCACAGGCCT GGCCTCGTCC CTGCAGGCTG GTCAACTCCT CCAGCAGACC
    TCCAGCAGCT GCTCCAGCTC CTACATCGTG CTGTGCATCG AGAACAGCTA CATGACACAG
    TCCAAGAAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001336124.1
    CDS51..4202
    Translation

    Target ORF information:

    RefSeq Version NM_001349195.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio collagen type XVIII alpha 1 chain a (col18a1a), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001349195.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGATGGCTG AACTCAGGTT TTGGCTGTGC CTCTTTATTT TGGCATGCGT GAGGATACGC 
    CACACACATG GCTGGTTCTG GTTTGATGAC TCCAAGGAAA TTGAGAAGGG CGTGCAGACA
    CCAGCATACC TGACCACCGT AGGGCCCACA TCCCTGCCGA GGACAGAACC ATCCAGAACA
    ACAGAAGAAA GTGGAGTGTC GCTGTTGCAA CTAATTGGCG ATCCTCCACC CAACGGCGTC
    TCCAAAGTCT TCGACCAAGC AAACAGTCCT GGATATGTGT TTGGACCAAG CTCAAATGTG
    GGTCAGTCTG CTGCAGCCCA CCTGCCCAAT CCCTTCTACC GCGATTTCTC CCTCATCTTC
    AATATAAAAC CCACATCCTC CAAGCCTGGC GTGATCTTCT CCATCACTGA CCCCACTCAG
    AACATCATGT ATGTTGGCGT GAAGCTGTCG GCGGTGGAAA GAGGCAAACA GTCCATTATC
    TTCTACTACA CCGAGCCTGA TTCGCAGAGC TCCTATGAGG CGGCCAGATT CTCTGTTTCC
    TCCATGTTGA ACATATGGAC GCGCTTTTCC ATCAGCGTTT TGAACGAGCA GGTTTCTCTC
    TACTTTAACT GCGACTCGGA TCCTCAGGTT GTCAATTTCG AGCGCTCGCC TGATGATATG
    GACATGGATG CTGGCGCTGG AGTGTTTGTG GGACATGCCA GTGGAGCAGA CCCGGATAAG
    TTTTTGGGTG TTATTGGTGA TCTGAGGGTG TCGAGAGACC CTGGGGCGGC TGAACGGCAC
    TGTGAAGAAG AGGAGGATGA CTATGCAACA GAGGCATCAG GTGATTACGG AGCAAGTGGA
    GATGGAGAGC AGCCATCACC AGTTCAGCCA ACACCTCCAT CATCTCGGCC AATCCAGCGG
    CCACCGGTGA TTAGCAGGGA TCCTATACAG CGGCCACCGG TGATTAGCAG GGAGCCTATA
    CAGCAGACAG TTGCAAGAGG AGAGAAGGGT GACAGAGGGG AGAAGGGTGC TAAAGGTGAC
    AGGGGTCTTG CTGGGCCAAA GGGTGATGTG GGGTCTGGAT CAGTGTCTGG TGGAGGATCA
    AAAGGAGACA AGGGCGTCCC AGGAGAGAAA GGAATGAAGG GCACTTCTGG TTTTGGTTAT
    CCTGGATCTA AGGGTGACCG TGGCCCCCCT GGACCTCCAG GACCTCCGGG TCCCCAAGGG
    CCCAGTGCAG AGGTAGAGGT GCGGGGCGAT GGGTCAGTTG TGCAGAAAGT CACAGGACCA
    AGAGGACCAC CTGGACCCCA AGGACCCCCT GGTCCCCCTG GACCTGAAGG GGAGCCGGGT
    GACCCTGGTG AAGATGGAAA AGCTGGCCAA GTTGGACCTT CTGGTTTTCC TGGAAATCCT
    GGGAATCCTG GACCCAAAGG AGATAAGGGA GATCGAGGTG AAAGTCAGCC AGGACCCCGT
    GGGCCGCCTG GTCCTCCCGG CCCACCAGGA CCCTCCTCTG GCTTTGATAG ACCTACGTTT
    GTGGACATGG AGGGCTCTGG ATTTGATATG GACAGTGTGC GGGCAGTGCC TGGTTTGCCT
    GGTCCACCTG GTCCTCCTGG TCCCCCTGGT CCCCCTGGCT CTGCATCTTC AGGCTCTGGT
    GGTTTTGGGC CACCTGGTCC TCCTGGGCAA AATGGGGCGC CAGGTCAGCC GGGACTTTCG
    GGTGTACCAG GTGCTGATGG AAAACCAGGC TTACCTGGTC CAAAAGGAGA GAAGGGTGAT
    GCCGGTGAGC TGGGCCTTCC TGGACCGGTG GGAGAGAAGG GTGCCAAAGG TTCTTCTGGT
    CCCCCTGGCA CCACTGGGAT TGGTGGGCCT GCTGGTCTTC CAGGACCAAT TGGACCTATT
    GGACTTCCAG GACCACCTGG TCCTCCTGGA CCAAGCTTTA ATGTTGGTTT TGATGACATG
    GAAGGTTCTG GTGTTCACTT CAGTTCAGTA CCTGGAGTAA GAGGACCAGT TGGAATCCCG
    GGGCCACCTG GTGTCCCAGG GCCACAGGGT CAGCCTGGAT TCCCAGGGCT TCCTGGTGAG
    AAAGGCAGTT TAGGAGCTCA GGGAAATGAT GGTCTTCCTG GTGCAGATGG CTTCCCTGGA
    CCACAGGGAC CAAAGGGCGA CAAAGGAGAG CGAGGCGACA GGGGTGAACC TGGTCGAGAT
    GGACATGGTC ATCCAGGCCA ACCAGGCCCT CCTGGACCAC CTGGACAGAT TATTTATCGC
    TACTCTGAAC ATTATGATGA AGTTGGAGGA ACGGGGGCTC AGGGTGGTTC TGGTGTTCCT
    GGCCAAGCAG GATTCCCAGG CCCAATGGGA CCAAAGGGTG ACAGAGGTGA GCCTGGTCTT
    CCAGGCTATG ACATTAAGGG GGAGAAAGGA GAACCTGGAC TTATTCTTGG ACCTGATGGA
    AATCCACTTT ACCTTGGTGG ATTGACAGGA CAGAAGGGTG AGAGAGGTAT TTCTGGACCA
    GTCGGACCTC CTGGTCCAGC AGGACCTCCT GGTGCAAAAG GTGAATTCGG AATGCCAGGC
    AGACCTGGTC GTCCAGGTGT GAACGGATAT AAAGGAGAGA AGGGAGAGCC AAGTTCAGGC
    TCTGGATATG GTTACCCGGG TCCCCCTGGT CCCCCAGGAC CCCCTGGTCC CCCCGGACCC
    GCTGTGCCCT TGGACAGATT TGGCAGATAT GAAGATTATG CAAGGCTCTA TCCAGCTACG
    AAAGGAGAGA AAGGAGACCA AGGAGCTCCT GGCATTCCCG GATCGCCAGG TTTTTCCACA
    AACTTTGATA TATATTCACT CAAGAATGAG ATGAAGGGTG AGCGGGGAGA GTCTGGTCTT
    AAAGGAGAGA AAGGAGAACC AGGAGGTGGA TTTTATGACC CTCGTTTTGG AGCTGTACAA
    GGACCACCAG GGAACCCAGG ACCTGCTGGA CCTAAAGGAG ACTCAATCAG AGGCCCACCT
    GGACCCCAGG GCCCACCAGG AACACCCGGG GTTGGTTATG ATGGTCGACC AGGTAATCCA
    GGACCTCCTG GACCACCTGG TCCACCAGGG TCCCCATCAC TGCCAGGACC CTATAGGCCA
    CCACTCAGTA TTCCTGGACC TCCAGGTCCT CCTGGCCCAC CTGGAATTCC TGGCACTGGA
    TCAGGGGTGA CTGTCCTGAG GTCTCATGAC ATAATGATTG CTACAGCACG CAGACAGACA
    GAAGGGACTC TGATTTACAT TCTGGACAGA AACGATCTCT ACCTCAGAGT TCGGGACGGT
    GTTAGACAAG TGATGCTTGG GGATTATAAA CCTCTTTATG GAGAGCTGGA CAATGAAGTG
    GCAGCAGTCC AGCCACCCCC TGTTGTTCAC TATTCCCAGG ACCACACGGC TAACAATGGC
    GCAGAGCAGA TTTCTCCACC ACACCAGCCT ATTGATTTCC CAACGAGAGA GCAGGAGAAC
    AGAAACCCCA ACCCGCAACC TGACCCCCGA TACCCGGATC CACGATACCC ACCATACACT
    GACAACAGAT ACACCGATCC TGTAGAGCCC AACATCAGAT ATCCTGTTCA ACCCGAGAGG
    AATCCAATCA CTCCAGCCCG CCGTCCTATC CCTCCTGTCA ATCAGCCAGG AGGCCACGCC
    CACACCCACA CTTCCGGACC AGGGTTGCAT CTTATTGCTC TGAATTCTCC ACAAGTGGGC
    AACATGAGAG GCATTCGCGG GGCAGACTTC ATGTGCTTCC AGCAGGCACG GGCCGTTGGC
    ATGAAGGGAA CGTTCAGAGC CTTCCTGTCC TCCAAACTCC AGGATCTCTA CAGTATCGTC
    CGCAGATCTG ACAGAGAGAC ATTACCAATT GTTAACCTAA AGGATCAAGT CCTCTTCAGC
    AGCTGGGAGT CTCTCTTCAG TGACTCAGAG AGCAGGATGA AGGACAATGC ACCCATCTAC
    TCGTTTGATG GCAGAGATGT CCTGAGGGAC AGTGCCTGGC CAGAGAAGAT GATTTGGCAC
    GGATCGGATG GCAGGGGTCA CAGGCAAACG GATAACTACT GCGAGACATG GAGGGCCGGA
    GACCGCGCTG TCACAGGCCT GGCCTCGTCC CTGCAGGCTG GTCAACTCCT CCAGCAGACC
    TCCAGCAGCT GCTCCAGCTC CTACATCGTG CTGTGCATCG AGAACAGCTA CATGACACAG
    TCCAAGAAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa143962
    Clone ID Related Accession (Same CDS sequence) XM_021478720.1
    Accession Version XM_021478720.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4938bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product collagen type XVIII, alpha 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATGGCTG AACTCAGGTT TTGGCTGTGC CTCTTTATTT TGGCATGCGT GAGGATACGC 
    CACACACATG GCTGGTTCTG GTTTGATGAC TCCAAGGAAA TTGAGAAGGG CGTGCAGACA
    CCAGCATACC TGACCACCGT AGGGCCCACA TCCCTGCCGA GGACAGAACC ATCCAGAACA
    ACAGGTACAA GTGCTTCTGT AACTGAAGAG GTCGCGAAAG AGGTCAGTTT TGAAGGGGAA
    CCGGAGGTCA GATCCAGGTC TGGATTGGGG ATTGAGTCCG GATCCGGATC TGGATCATCC
    AGTTTTGAAA GACAGCATGA ATATTTAACA TCATCCGACA AAATTTGGGG TATTATTGAT
    GACAATTCTA GTTTGCATAA TCAAGTGACC AAAGTGGACC AGACGGATGG ACTTGACCCA
    GCAAACAGTC GATTTAATGA AGGCGTTAAT GTTTCTTTCA CAAATATTAC TGCTAAAAAT
    AATGTAAATG AGAGTAATTT TGCAGAGCAT GCTAACACGA CGTTGTATTC CTCTGAATAC
    GCTGTCTCAG ATAATGCACT AACCATTGAA AGCCAATTTT TAATAGCCAG CATGCCAGCA
    TCCTTCGAAC CCCTGCGCTG CTTGCCGGTC GATTCTGGTT TGCCGTTTTG CACCAGAAGG
    GGAGTGGAGA GTTTCTCTGT GCCAAATTTT CTAAATCAAA GCAGCGTGGA GGAAGTTCGG
    ATGGTTTTGA CCGAATGGGC ATGGCTTTTG AGGTCAGGCT GTCATCATAG TTTGGAATGG
    TTTTTCTGCC TGCTGTTGAC CCCGCGGTGT GGCCACTCTG GATCTCTGCT GCCCTGTCGC
    AGTTCTTGTG AGGTCCTGCA GGACAGTTGC TGGACACTCC TGGACGAGGG CCGCCTCCCT
    GTGGAGTGCA AATCTCTGCC TGAAGAGAAG CATGATGGGT ATCGGTGCTT GTCAGTTAGT
    AACCTGAAAG AAGAAAGTGG AGTGTCGCTG TTGCAACTAA TTGGCGATCC TCCACCCAAC
    GGCGTCTCCA AAGTCTTCGA CCAAGCAAAC AGTCCTGGAT ATGTGTTTGG ACCAAGCTCA
    AATGTGGGTC AGTCTGCTGC AGCCCACCTG CCCAATCCCT TCTACCGCGA TTTCTCCCTC
    ATCTTCAATA TAAAACCCAC ATCCTCCAAG CCTGGCGTGA TCTTCTCCAT CACTGACCCC
    ACTCAGAACA TCATGTATGT TGGCGTGAAG CTGTCGGCGG TGGAAAGAGG CAAACAGTCC
    ATTATCTTCT ACTACACCGA GCCTGATTCG CAGAGCTCCT ATGAGGCGGC CAGATTCTCT
    GTTTCCTCCA TGTTGAACAT ATGGACGCGC TTTTCCATCA GCGTTTTGAA CGAGCAGGTT
    TCTCTCTACT TTAACTGCGA CTCGGATCCT CAGGTTGTCA ATTTCGAGCG CTCGCCTGAT
    GATATGGACA TGGATGCTGG CGCTGGAGTG TTTGTGGGAC ATGCCAGTGG AGCAGACCCG
    GATAAGTTTT TGGGTGTTAT TGGTGATCTG AGGGTGTCGA GAGACCCTGG GGCGGCTGAA
    CGGCACTGTG AAGAAGAGGA GGATGACTAT GCAACAGAGG CATCAGGTGA TTACGGAGCA
    AGTGGAGATG GAGAGCAGCC ATCACCAGTT CAGCCAACAC CTCCATCATC TCGGCCAATC
    CAGCGGCCAC CGGTGATTAG CAGGGATCCT ATACAGCGGC CACCGGTGAT TAGCAGGGAG
    CCTATACAGC AGACAGTTGC AAGAGGAGAG AAGGGTGACA GAGGGGAGAA GGGTGCTAAA
    GGTGACAGGG GTCTTGCTGG GCCAAAGGGT GATGTGGGGT CTGGATCAGT GTCTGGTGGA
    GGATCAAAAG GAGACAAGGG CGTCCCAGGA GAGAAAGGAA TGAAGGGCAC TTCTGGTTTT
    GGTTATCCTG GATCTAAGGG TGACCGTGGC CCCCCTGGAC CTCCAGGACC TCCGGGTCCC
    CAAGGGCCCA GTGCAGAGGT AGAGGTGCGG GGCGATGGGT CAGTTGTGCA GAAAGTCACA
    GGACCAAGAG GACCACCTGG ACCCCAAGGA CCCCCTGGTC CCCCTGGACC TGAAGGGGAG
    CCGGGTGACC CTGGTGAAGA TGGAAAAGCT GGCCAAGTTG GACCTTCTGG TTTTCCTGGA
    AATCCTGGGA ATCCTGGACC CAAAGGAGAT AAGGGAGATC GAGGTGAAAG TCAGCCAGGA
    CCCCGTGGGC CGCCTGGTCC TCCCGGCCCA CCAGGACCCT CCTCTGGCTT TGATAGACCT
    ACGTTTGTGG ACATGGAGGG CTCTGGATTT GATATGGACA GTGTGCGGGC AGTGCCTGGT
    TTGCCTGGTC CACCTGGTCC TCCTGGTCCC CCTGGTCCCC CTGGCTCTGC ATCTTCAGGC
    TCTGGTGGTT TTGGGCCACC TGGTCCTCCT GGGCAAAATG GGGCGCCAGG TCAGCCGGGA
    CTTTCGGGTG TACCAGGTGC TGATGGAAAA CCAGGCTTAC CTGGTCCAAA AGGAGAGAAG
    GGTGATGCCG GTGAGCTGGG CCTTCCTGGA CCGGTGGGAG AGAAGGGTGC CAAAGGTTCT
    TCTGGTCCCC CTGGCACCAC TGGGATTGGT GGGCCTGCTG GTCTTCCAGG ACCAATTGGA
    CCTATTGGAC TTCCAGGACC ACCTGGTCCT CCTGGACCAA GCTTTAATGT TGGTTTTGAT
    GACATGGAAG GTTCTGGTGT TCACTTCAGT TCAGTACCTG GAGTAAGAGG ACCAGTTGGA
    ATCCCGGGGC CACCTGGTGT CCCAGGGCCA CAGGGTCAGC CTGGATTCCC AGGGCTTCCT
    GGTGAGAAAG GCAGTTTAGG AGCTCAGGGA AATGATGGTC TTCCTGGTGC AGATGGCTTC
    CCTGGACCAC AGGGACCAAA GGGCGACAAA GGAGAGCGAG GCGACAGGGG TGAACCTGGT
    CGAGATGGAC ATGGTCATCC AGGCCAACCA GGCCCTCCTG GACCACCTGG ACAGATTATT
    TATCGCTACT CTGAACATTA TGATGAAGTT GGAGGAACGG GGGCTCAGGG TGGTTCTGGT
    GTTCCTGGCC AAGCAGGATT CCCAGGCCCA ATGGGACCAA AGGGTGACAG AGGTGAGCCT
    GGTCTTCCAG GCTATGACAT TAAGGGGGAG AAAGGAGAAC CTGGACTTAT TCTTGGACCT
    GATGGAAATC CACTTTACCT TGGTGGATTG ACAGGACAGA AGGGTGAGAG AGGTATTTCT
    GGACCAGTCG GACCTCCTGG TCCAGCAGGA CCTCCTGGTG CAAAAGGTGA ATTCGGAATG
    CCAGGCAGAC CTGGTCGTCC AGGTGTGAAC GGATATAAAG GAGAGAAGGG AGAGCCAAGT
    TCAGGCTCTG GATATGGTTA CCCGGGTCCC CCTGGTCCCC CAGGACCCCC TGGTCCCCCC
    GGACCCGCTG TGCCCTTGGA CAGATTTGGC AGATATGAAG ATTATGCAAG GCTCTATCCA
    GCTACGAAAG GAGAGAAAGG AGACCAAGGA GCTCCTGGCA TTCCCGGATC GCCAGGTTTT
    TCCACAAACT TTGATATATA TTCACTCAAG AATGAGATGA AGGGTGAGCG GGGAGAGTCT
    GGTCTTAAAG GAGAGAAAGG AGAACCAGGA GGTGGATTTT ATGACCCTCG TTTTGGAGCT
    GTACAAGGAC CACCAGGGAA CCCAGGACCT GCTGGACCTA AAGGAGACTC AATCAGAGGC
    CCACCTGGAC CCCAGGGCCC ACCAGGAACA CCCGGGGTTG GTTATGATGG TCGACCAGGT
    AATCCAGGAC CTCCTGGACC ACCTGGTCCA CCAGGGTCCC CATCACTGCC AGGACCCTAT
    AGGCCACCAC TCAGTATTCC TGGACCTCCA GGTCCTCCTG GCCCACCTGG AATTCCTGGC
    ACTGGATCAG GGGTGACTGT CCTGAGGTCT CATGACATAA TGATTGCTAC AGCACGCAGA
    CAGACAGAAG GGACTCTGAT TTACATTCTG GACAGAAACG ATCTCTACCT CAGAGTTCGG
    GACGGTGTTA GACAAGTGAT GCTTGGGGAT TATAAACCTC TTTATGGAGA GCTGGACAAT
    GAAGTGGCAG CAGTCCAGCC ACCCCCTGTT GTTCACTATT CCCAGGACCA CACGGCTAAC
    AATGGCGCAG AGCAGATTTC TCCACCACAC CAGCCTATTG ATTTCCCAAC GAGAGAGCAG
    GAGAACAGAA ACCCCAACCC GCAACCTGAC CCCCGATACC CGGATCCACG ATACCCACCA
    TACACTGACA ACAGATACAC CGATCCTGTA GAGCCCAACA TCAGATATCC TGTTCAACCC
    GAGAGGAATC CAATCACTCC AGCCCGCCGT CCTATCCCTC CTGTCAATCA GCCAGGAGGC
    CACGCCCACA CCCACACTTC CGGACCAGGG TTGCATCTTA TTGCTCTGAA TTCTCCACAA
    GTGGGCAACA TGAGAGGCAT TCGCGGGGCA GACTTCATGT GCTTCCAGCA GGCACGGGCC
    GTTGGCATGA AGGGAACGTT CAGAGCCTTC CTGTCCTCCA AACTCCAGGA TCTCTACAGT
    ATCGTCCGCA GATCTGACAG AGAGACATTA CCAATTGTTA ACCTAAAGGA TCAAGTCCTC
    TTCAGCAGCT GGGAGTCTCT CTTCAGTGAC TCAGAGAGCA GGATGAAGGA CAATGCACCC
    ATCTACTCGT TTGATGGCAG AGATGTCCTG AGGGACAGTG CCTGGCCAGA GAAGATGATT
    TGGCACGGAT CGGATGGCAG GGGTCACAGG CAAACGGATA ACTACTGCGA GACATGGAGG
    GCCGGAGACC GCGCTGTCAC AGGCCTGGCC TCGTCCCTGC AGGCTGGTCA ACTCCTCCAG
    CAGACCTCCA GCAGCTGCTC CAGCTCCTAC ATCGTGCTGT GCATCGAGAA CAGCTACATG
    ACACAGTCCA AGAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021334395.1
    CDS66..5003
    Translation

    Target ORF information:

    RefSeq Version XM_021478720.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio collagen type XVIII alpha 1 chain a (col18a1a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021478720.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGATGGCTG AACTCAGGTT TTGGCTGTGC CTCTTTATTT TGGCATGCGT GAGGATACGC 
    CACACACATG GCTGGTTCTG GTTTGATGAC TCCAAGGAAA TTGAGAAGGG CGTGCAGACA
    CCAGCATACC TGACCACCGT AGGGCCCACA TCCCTGCCGA GGACAGAACC ATCCAGAACA
    ACAGGTACAA GTGCTTCTGT AACTGAAGAG GTCGCGAAAG AGGTCAGTTT TGAAGGGGAA
    CCGGAGGTCA GATCCAGGTC TGGATTGGGG ATTGAGTCCG GATCCGGATC TGGATCATCC
    AGTTTTGAAA GACAGCATGA ATATTTAACA TCATCCGACA AAATTTGGGG TATTATTGAT
    GACAATTCTA GTTTGCATAA TCAAGTGACC AAAGTGGACC AGACGGATGG ACTTGACCCA
    GCAAACAGTC GATTTAATGA AGGCGTTAAT GTTTCTTTCA CAAATATTAC TGCTAAAAAT
    AATGTAAATG AGAGTAATTT TGCAGAGCAT GCTAACACGA CGTTGTATTC CTCTGAATAC
    GCTGTCTCAG ATAATGCACT AACCATTGAA AGCCAATTTT TAATAGCCAG CATGCCAGCA
    TCCTTCGAAC CCCTGCGCTG CTTGCCGGTC GATTCTGGTT TGCCGTTTTG CACCAGAAGG
    GGAGTGGAGA GTTTCTCTGT GCCAAATTTT CTAAATCAAA GCAGCGTGGA GGAAGTTCGG
    ATGGTTTTGA CCGAATGGGC ATGGCTTTTG AGGTCAGGCT GTCATCATAG TTTGGAATGG
    TTTTTCTGCC TGCTGTTGAC CCCGCGGTGT GGCCACTCTG GATCTCTGCT GCCCTGTCGC
    AGTTCTTGTG AGGTCCTGCA GGACAGTTGC TGGACACTCC TGGACGAGGG CCGCCTCCCT
    GTGGAGTGCA AATCTCTGCC TGAAGAGAAG CATGATGGGT ATCGGTGCTT GTCAGTTAGT
    AACCTGAAAG AAGAAAGTGG AGTGTCGCTG TTGCAACTAA TTGGCGATCC TCCACCCAAC
    GGCGTCTCCA AAGTCTTCGA CCAAGCAAAC AGTCCTGGAT ATGTGTTTGG ACCAAGCTCA
    AATGTGGGTC AGTCTGCTGC AGCCCACCTG CCCAATCCCT TCTACCGCGA TTTCTCCCTC
    ATCTTCAATA TAAAACCCAC ATCCTCCAAG CCTGGCGTGA TCTTCTCCAT CACTGACCCC
    ACTCAGAACA TCATGTATGT TGGCGTGAAG CTGTCGGCGG TGGAAAGAGG CAAACAGTCC
    ATTATCTTCT ACTACACCGA GCCTGATTCG CAGAGCTCCT ATGAGGCGGC CAGATTCTCT
    GTTTCCTCCA TGTTGAACAT ATGGACGCGC TTTTCCATCA GCGTTTTGAA CGAGCAGGTT
    TCTCTCTACT TTAACTGCGA CTCGGATCCT CAGGTTGTCA ATTTCGAGCG CTCGCCTGAT
    GATATGGACA TGGATGCTGG CGCTGGAGTG TTTGTGGGAC ATGCCAGTGG AGCAGACCCG
    GATAAGTTTT TGGGTGTTAT TGGTGATCTG AGGGTGTCGA GAGACCCTGG GGCGGCTGAA
    CGGCACTGTG AAGAAGAGGA GGATGACTAT GCAACAGAGG CATCAGGTGA TTACGGAGCA
    AGTGGAGATG GAGAGCAGCC ATCACCAGTT CAGCCAACAC CTCCATCATC TCGGCCAATC
    CAGCGGCCAC CGGTGATTAG CAGGGATCCT ATACAGCGGC CACCGGTGAT TAGCAGGGAG
    CCTATACAGC AGACAGTTGC AAGAGGAGAG AAGGGTGACA GAGGGGAGAA GGGTGCTAAA
    GGTGACAGGG GTCTTGCTGG GCCAAAGGGT GATGTGGGGT CTGGATCAGT GTCTGGTGGA
    GGATCAAAAG GAGACAAGGG CGTCCCAGGA GAGAAAGGAA TGAAGGGCAC TTCTGGTTTT
    GGTTATCCTG GATCTAAGGG TGACCGTGGC CCCCCTGGAC CTCCAGGACC TCCGGGTCCC
    CAAGGGCCCA GTGCAGAGGT AGAGGTGCGG GGCGATGGGT CAGTTGTGCA GAAAGTCACA
    GGACCAAGAG GACCACCTGG ACCCCAAGGA CCCCCTGGTC CCCCTGGACC TGAAGGGGAG
    CCGGGTGACC CTGGTGAAGA TGGAAAAGCT GGCCAAGTTG GACCTTCTGG TTTTCCTGGA
    AATCCTGGGA ATCCTGGACC CAAAGGAGAT AAGGGAGATC GAGGTGAAAG TCAGCCAGGA
    CCCCGTGGGC CGCCTGGTCC TCCCGGCCCA CCAGGACCCT CCTCTGGCTT TGATAGACCT
    ACGTTTGTGG ACATGGAGGG CTCTGGATTT GATATGGACA GTGTGCGGGC AGTGCCTGGT
    TTGCCTGGTC CACCTGGTCC TCCTGGTCCC CCTGGTCCCC CTGGCTCTGC ATCTTCAGGC
    TCTGGTGGTT TTGGGCCACC TGGTCCTCCT GGGCAAAATG GGGCGCCAGG TCAGCCGGGA
    CTTTCGGGTG TACCAGGTGC TGATGGAAAA CCAGGCTTAC CTGGTCCAAA AGGAGAGAAG
    GGTGATGCCG GTGAGCTGGG CCTTCCTGGA CCGGTGGGAG AGAAGGGTGC CAAAGGTTCT
    TCTGGTCCCC CTGGCACCAC TGGGATTGGT GGGCCTGCTG GTCTTCCAGG ACCAATTGGA
    CCTATTGGAC TTCCAGGACC ACCTGGTCCT CCTGGACCAA GCTTTAATGT TGGTTTTGAT
    GACATGGAAG GTTCTGGTGT TCACTTCAGT TCAGTACCTG GAGTAAGAGG ACCAGTTGGA
    ATCCCGGGGC CACCTGGTGT CCCAGGGCCA CAGGGTCAGC CTGGATTCCC AGGGCTTCCT
    GGTGAGAAAG GCAGTTTAGG AGCTCAGGGA AATGATGGTC TTCCTGGTGC AGATGGCTTC
    CCTGGACCAC AGGGACCAAA GGGCGACAAA GGAGAGCGAG GCGACAGGGG TGAACCTGGT
    CGAGATGGAC ATGGTCATCC AGGCCAACCA GGCCCTCCTG GACCACCTGG ACAGATTATT
    TATCGCTACT CTGAACATTA TGATGAAGTT GGAGGAACGG GGGCTCAGGG TGGTTCTGGT
    GTTCCTGGCC AAGCAGGATT CCCAGGCCCA ATGGGACCAA AGGGTGACAG AGGTGAGCCT
    GGTCTTCCAG GCTATGACAT TAAGGGGGAG AAAGGAGAAC CTGGACTTAT TCTTGGACCT
    GATGGAAATC CACTTTACCT TGGTGGATTG ACAGGACAGA AGGGTGAGAG AGGTATTTCT
    GGACCAGTCG GACCTCCTGG TCCAGCAGGA CCTCCTGGTG CAAAAGGTGA ATTCGGAATG
    CCAGGCAGAC CTGGTCGTCC AGGTGTGAAC GGATATAAAG GAGAGAAGGG AGAGCCAAGT
    TCAGGCTCTG GATATGGTTA CCCGGGTCCC CCTGGTCCCC CAGGACCCCC TGGTCCCCCC
    GGACCCGCTG TGCCCTTGGA CAGATTTGGC AGATATGAAG ATTATGCAAG GCTCTATCCA
    GCTACGAAAG GAGAGAAAGG AGACCAAGGA GCTCCTGGCA TTCCCGGATC GCCAGGTTTT
    TCCACAAACT TTGATATATA TTCACTCAAG AATGAGATGA AGGGTGAGCG GGGAGAGTCT
    GGTCTTAAAG GAGAGAAAGG AGAACCAGGA GGTGGATTTT ATGACCCTCG TTTTGGAGCT
    GTACAAGGAC CACCAGGGAA CCCAGGACCT GCTGGACCTA AAGGAGACTC AATCAGAGGC
    CCACCTGGAC CCCAGGGCCC ACCAGGAACA CCCGGGGTTG GTTATGATGG TCGACCAGGT
    AATCCAGGAC CTCCTGGACC ACCTGGTCCA CCAGGGTCCC CATCACTGCC AGGACCCTAT
    AGGCCACCAC TCAGTATTCC TGGACCTCCA GGTCCTCCTG GCCCACCTGG AATTCCTGGC
    ACTGGATCAG GGGTGACTGT CCTGAGGTCT CATGACATAA TGATTGCTAC AGCACGCAGA
    CAGACAGAAG GGACTCTGAT TTACATTCTG GACAGAAACG ATCTCTACCT CAGAGTTCGG
    GACGGTGTTA GACAAGTGAT GCTTGGGGAT TATAAACCTC TTTATGGAGA GCTGGACAAT
    GAAGTGGCAG CAGTCCAGCC ACCCCCTGTT GTTCACTATT CCCAGGACCA CACGGCTAAC
    AATGGCGCAG AGCAGATTTC TCCACCACAC CAGCCTATTG ATTTCCCAAC GAGAGAGCAG
    GAGAACAGAA ACCCCAACCC GCAACCTGAC CCCCGATACC CGGATCCACG ATACCCACCA
    TACACTGACA ACAGATACAC CGATCCTGTA GAGCCCAACA TCAGATATCC TGTTCAACCC
    GAGAGGAATC CAATCACTCC AGCCCGCCGT CCTATCCCTC CTGTCAATCA GCCAGGAGGC
    CACGCCCACA CCCACACTTC CGGACCAGGG TTGCATCTTA TTGCTCTGAA TTCTCCACAA
    GTGGGCAACA TGAGAGGCAT TCGCGGGGCA GACTTCATGT GCTTCCAGCA GGCACGGGCC
    GTTGGCATGA AGGGAACGTT CAGAGCCTTC CTGTCCTCCA AACTCCAGGA TCTCTACAGT
    ATCGTCCGCA GATCTGACAG AGAGACATTA CCAATTGTTA ACCTAAAGGA TCAAGTCCTC
    TTCAGCAGCT GGGAGTCTCT CTTCAGTGAC TCAGAGAGCA GGATGAAGGA CAATGCACCC
    ATCTACTCGT TTGATGGCAG AGATGTCCTG AGGGACAGTG CCTGGCCAGA GAAGATGATT
    TGGCACGGAT CGGATGGCAG GGGTCACAGG CAAACGGATA ACTACTGCGA GACATGGAGG
    GCCGGAGACC GCGCTGTCAC AGGCCTGGCC TCGTCCCTGC AGGCTGGTCA ACTCCTCCAG
    CAGACCTCCA GCAGCTGCTC CAGCTCCTAC ATCGTGCTGT GCATCGAGAA CAGCTACATG
    ACACAGTCCA AGAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa143963
    Clone ID Related Accession (Same CDS sequence) XM_021478721.1
    Accession Version XM_021478721.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4314bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product collagen type XVIII, alpha 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCCAGCAT CCTTCGAACC CCTGCGCTGC TTGCCGGTCG ATTCTGGTTT GCCGTTTTGC 
    ACCAGAAGGG GAGTGGAGAG TTTCTCTGTG CCAAATTTTC TAAATCAAAG CAGCGTGGAG
    GAAGTTCGGA TGGTTTTGAC CGAATGGGCA TGGCTTTTGA GGTCAGGCTG TCATCATAGT
    TTGGAATGGT TTTTCTGCCT GCTGTTGACC CCGCGGTGTG GCCACTCTGG ATCTCTGCTG
    CCCTGTCGCA GTTCTTGTGA GGTCCTGCAG GACAGTTGCT GGACACTCCT GGACGAGGGC
    CGCCTCCCTG TGGAGTGCAA ATCTCTGCCT GAAGAGAAGC ATGATGGGTA TCGGTGCTTG
    TCAGTTAGTA ACCTGAAAGA AGAAAGTGGA GTGTCGCTGT TGCAACTAAT TGGCGATCCT
    CCACCCAACG GCGTCTCCAA AGTCTTCGAC CAAGCAAACA GTCCTGGATA TGTGTTTGGA
    CCAAGCTCAA ATGTGGGTCA GTCTGCTGCA GCCCACCTGC CCAATCCCTT CTACCGCGAT
    TTCTCCCTCA TCTTCAATAT AAAACCCACA TCCTCCAAGC CTGGCGTGAT CTTCTCCATC
    ACTGACCCCA CTCAGAACAT CATGTATGTT GGCGTGAAGC TGTCGGCGGT GGAAAGAGGC
    AAACAGTCCA TTATCTTCTA CTACACCGAG CCTGATTCGC AGAGCTCCTA TGAGGCGGCC
    AGATTCTCTG TTTCCTCCAT GTTGAACATA TGGACGCGCT TTTCCATCAG CGTTTTGAAC
    GAGCAGGTTT CTCTCTACTT TAACTGCGAC TCGGATCCTC AGGTTGTCAA TTTCGAGCGC
    TCGCCTGATG ATATGGACAT GGATGCTGGC GCTGGAGTGT TTGTGGGACA TGCCAGTGGA
    GCAGACCCGG ATAAGTTTTT GGGTGTTATT GGTGATCTGA GGGTGTCGAG AGACCCTGGG
    GCGGCTGAAC GGCACTGTGA AGAAGAGGAG GATGACTATG CAACAGAGGC ATCAGGTGAT
    TACGGAGCAA GTGGAGATGG AGAGCAGCCA TCACCAGTTC AGCCAACACC TCCATCATCT
    CGGCCAATCC AGCGGCCACC GGTGATTAGC AGGGAGCCTA TACAGCAGAC AGTTGCAAGA
    GGAGAGAAGG GTGACAGAGG GGAGAAGGGT GCTAAAGGTG ACAGGGGTCT TGCTGGGCCA
    AAGGGTGATG TGGGGTCTGG ATCAGTGTCT GGTGGAGGAT CAAAAGGAGA CAAGGGCGTC
    CCAGGAGAGA AAGGAATGAA GGGCACTTCT GGTTTTGGTT ATCCTGGATC TAAGGGTGAC
    CGTGGCCCCC CTGGACCTCC AGGACCTCCG GGTCCCCAAG GGCCCAGTGC AGAGGTAGAG
    GTGCGGGGCG ATGGGTCAGT TGTGCAGAAA GTCACAGGAC CAAGAGGACC ACCTGGACCC
    CAAGGACCCC CTGGTCCCCC TGGACCTGAA GGGGAGCCGG GTGACCCTGG TGAAGATGGA
    AAAGCTGGCC AAGTTGGACC TTCTGGTTTT CCTGGAAATC CTGGGAATCC TGGACCCAAA
    GGAGATAAGG GAGATCGAGG TGAAAGTCAG CCAGGACCCC GTGGGCCGCC TGGTCCTCCC
    GGCCCACCAG GACCCTCCTC TGGCTTTGAT AGACCTACGT TTGTGGACAT GGAGGGCTCT
    GGATTTGATA TGGACAGTGT GCGGGCAGTG CCTGGTTTGC CTGGTCCACC TGGTCCTCCT
    GGTCCCCCTG GTCCCCCTGG CTCTGCATCT TCAGGCTCTG GTGGTTTTGG GCCACCTGGT
    CCTCCTGGGC AAAATGGGGC GCCAGGTCAG CCGGGACTTT CGGGTGTACC AGGTGCTGAT
    GGAAAACCAG GCTTACCTGG TCCAAAAGGA GAGAAGGGTG ATGCCGGTGA GCTGGGCCTT
    CCTGGACCGG TGGGAGAGAA GGGTGCCAAA GGTTCTTCTG GTCCCCCTGG CACCACTGGG
    ATTGGTGGGC CTGCTGGTCT TCCAGGACCA ATTGGACCTA TTGGACTTCC AGGACCACCT
    GGTCCTCCTG GACCAAGCTT TAATGTTGGT TTTGATGACA TGGAAGGTTC TGGTGTTCAC
    TTCAGTTCAG TACCTGGAGT AAGAGGACCA GTTGGAATCC CGGGGCCACC TGGTGTCCCA
    GGGCCACAGG GTCAGCCTGG ATTCCCAGGG CTTCCTGGTG AGAAAGGCAG TTTAGGAGCT
    CAGGGAAATG ATGGTCTTCC TGGTGCAGAT GGCTTCCCTG GACCACAGGG ACCAAAGGGC
    GACAAAGGAG AGCGAGGCGA CAGGGGTGAA CCTGGTCGAG ATGGACATGG TCATCCAGGC
    CAACCAGGCC CTCCTGGACC ACCTGGACAG ATTATTTATC GCTACTCTGA ACATTATGAT
    GAAGTTGGAG GAACGGGGGC TCAGGGTGGT TCTGGTGTTC CTGGCCAAGC AGGATTCCCA
    GGCCCAATGG GACCAAAGGG TGACAGAGGT GAGCCTGGTC TTCCAGGCTA TGACATTAAG
    GGGGAGAAAG GAGAACCTGG ACTTATTCTT GGACCTGATG GAAATCCACT TTACCTTGGT
    GGATTGACAG GACAGAAGGG TGAGAGAGGT ATTTCTGGAC CAGTCGGACC TCCTGGTCCA
    GCAGGACCTC CTGGTGCAAA AGGTGAATTC GGAATGCCAG GCAGACCTGG TCGTCCAGGT
    GTGAACGGAT ATAAAGGAGA GAAGGGAGAG CCAAGTTCAG GCTCTGGATA TGGTTACCCG
    GGTCCCCCTG GTCCCCCAGG ACCCCCTGGT CCCCCCGGAC CCGCTGTGCC CTTGGACAGA
    TTTGGCAGAT ATGAAGATTA TGCAAGGCTC TATCCAGCTA CGAAAGGAGA GAAAGGAGAC
    CAAGGAGCTC CTGGCATTCC CGGATCGCCA GGTTTTTCCA CAAACTTTGA TATATATTCA
    CTCAAGAATG AGATGAAGGG TGAGCGGGGA GAGTCTGGTC TTAAAGGAGA GAAAGGAGAA
    CCAGGAGGTG GATTTTATGA CCCTCGTTTT GGAGCTGTAC AAGGACCACC AGGGAACCCA
    GGACCTGCTG GACCTAAAGG AGACTCAATC AGAGGCCCAC CTGGACCCCA GGGCCCACCA
    GGAACACCCG GGGTTGGTTA TGATGGTCGA CCAGGTAATC CAGGACCTCC TGGACCACCT
    GGTCCACCAG GGTCCCCATC ACTGCCAGGA CCCTATAGGC CACCACTCAG TATTCCTGGA
    CCTCCAGGTC CTCCTGGCCC ACCTGGAATT CCTGGCACTG GATCAGGGGT GACTGTCCTG
    AGGTCTCATG ACATAATGAT TGCTACAGCA CGCAGACAGA CAGAAGGGAC TCTGATTTAC
    ATTCTGGACA GAAACGATCT CTACCTCAGA GTTCGGGACG GTGTTAGACA AGTGATGCTT
    GGGGATTATA AACCTCTTTA TGGAGAGCTG GACAATGAAG TGGCAGCAGT CCAGCCACCC
    CCTGTTGTTC ACTATTCCCA GGACCACACG GCTAACAATG GCGCAGAGCA GATTTCTCCA
    CCACACCAGC CTATTGATTT CCCAACGAGA GAGCAGGAGA ACAGAAACCC CAACCCGCAA
    CCTGACCCCC GATACCCGGA TCCACGATAC CCACCATACA CTGACAACAG ATACACCGAT
    CCTGTAGAGC CCAACATCAG ATATCCTGTT CAACCCGAGA GGAATCCAAT CACTCCAGCC
    CGCCGTCCTA TCCCTCCTGT CAATCAGCCA GGAGGCCACG CCCACACCCA CACTTCCGGA
    CCAGGGTTGC ATCTTATTGC TCTGAATTCT CCACAAGTGG GCAACATGAG AGGCATTCGC
    GGGGCAGACT TCATGTGCTT CCAGCAGGCA CGGGCCGTTG GCATGAAGGG AACGTTCAGA
    GCCTTCCTGT CCTCCAAACT CCAGGATCTC TACAGTATCG TCCGCAGATC TGACAGAGAG
    ACATTACCAA TTGTTAACCT AAAGGATCAA GTCCTCTTCA GCAGCTGGGA GTCTCTCTTC
    AGTGACTCAG AGAGCAGGAT GAAGGACAAT GCACCCATCT ACTCGTTTGA TGGCAGAGAT
    GTCCTGAGGG ACAGTGCCTG GCCAGAGAAG ATGATTTGGC ACGGATCGGA TGGCAGGGGT
    CACAGGCAAA CGGATAACTA CTGCGAGACA TGGAGGGCCG GAGACCGCGC TGTCACAGGC
    CTGGCCTCGT CCCTGCAGGC TGGTCAACTC CTCCAGCAGA CCTCCAGCAG CTGCTCCAGC
    TCCTACATCG TGCTGTGCAT CGAGAACAGC TACATGACAC AGTCCAAGAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021334396.1
    CDS311..4624
    Translation

    Target ORF information:

    RefSeq Version XM_021478721.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio collagen type XVIII alpha 1 chain a (col18a1a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021478721.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGCCAGCAT CCTTCGAACC CCTGCGCTGC TTGCCGGTCG ATTCTGGTTT GCCGTTTTGC 
    ACCAGAAGGG GAGTGGAGAG TTTCTCTGTG CCAAATTTTC TAAATCAAAG CAGCGTGGAG
    GAAGTTCGGA TGGTTTTGAC CGAATGGGCA TGGCTTTTGA GGTCAGGCTG TCATCATAGT
    TTGGAATGGT TTTTCTGCCT GCTGTTGACC CCGCGGTGTG GCCACTCTGG ATCTCTGCTG
    CCCTGTCGCA GTTCTTGTGA GGTCCTGCAG GACAGTTGCT GGACACTCCT GGACGAGGGC
    CGCCTCCCTG TGGAGTGCAA ATCTCTGCCT GAAGAGAAGC ATGATGGGTA TCGGTGCTTG
    TCAGTTAGTA ACCTGAAAGA AGAAAGTGGA GTGTCGCTGT TGCAACTAAT TGGCGATCCT
    CCACCCAACG GCGTCTCCAA AGTCTTCGAC CAAGCAAACA GTCCTGGATA TGTGTTTGGA
    CCAAGCTCAA ATGTGGGTCA GTCTGCTGCA GCCCACCTGC CCAATCCCTT CTACCGCGAT
    TTCTCCCTCA TCTTCAATAT AAAACCCACA TCCTCCAAGC CTGGCGTGAT CTTCTCCATC
    ACTGACCCCA CTCAGAACAT CATGTATGTT GGCGTGAAGC TGTCGGCGGT GGAAAGAGGC
    AAACAGTCCA TTATCTTCTA CTACACCGAG CCTGATTCGC AGAGCTCCTA TGAGGCGGCC
    AGATTCTCTG TTTCCTCCAT GTTGAACATA TGGACGCGCT TTTCCATCAG CGTTTTGAAC
    GAGCAGGTTT CTCTCTACTT TAACTGCGAC TCGGATCCTC AGGTTGTCAA TTTCGAGCGC
    TCGCCTGATG ATATGGACAT GGATGCTGGC GCTGGAGTGT TTGTGGGACA TGCCAGTGGA
    GCAGACCCGG ATAAGTTTTT GGGTGTTATT GGTGATCTGA GGGTGTCGAG AGACCCTGGG
    GCGGCTGAAC GGCACTGTGA AGAAGAGGAG GATGACTATG CAACAGAGGC ATCAGGTGAT
    TACGGAGCAA GTGGAGATGG AGAGCAGCCA TCACCAGTTC AGCCAACACC TCCATCATCT
    CGGCCAATCC AGCGGCCACC GGTGATTAGC AGGGAGCCTA TACAGCAGAC AGTTGCAAGA
    GGAGAGAAGG GTGACAGAGG GGAGAAGGGT GCTAAAGGTG ACAGGGGTCT TGCTGGGCCA
    AAGGGTGATG TGGGGTCTGG ATCAGTGTCT GGTGGAGGAT CAAAAGGAGA CAAGGGCGTC
    CCAGGAGAGA AAGGAATGAA GGGCACTTCT GGTTTTGGTT ATCCTGGATC TAAGGGTGAC
    CGTGGCCCCC CTGGACCTCC AGGACCTCCG GGTCCCCAAG GGCCCAGTGC AGAGGTAGAG
    GTGCGGGGCG ATGGGTCAGT TGTGCAGAAA GTCACAGGAC CAAGAGGACC ACCTGGACCC
    CAAGGACCCC CTGGTCCCCC TGGACCTGAA GGGGAGCCGG GTGACCCTGG TGAAGATGGA
    AAAGCTGGCC AAGTTGGACC TTCTGGTTTT CCTGGAAATC CTGGGAATCC TGGACCCAAA
    GGAGATAAGG GAGATCGAGG TGAAAGTCAG CCAGGACCCC GTGGGCCGCC TGGTCCTCCC
    GGCCCACCAG GACCCTCCTC TGGCTTTGAT AGACCTACGT TTGTGGACAT GGAGGGCTCT
    GGATTTGATA TGGACAGTGT GCGGGCAGTG CCTGGTTTGC CTGGTCCACC TGGTCCTCCT
    GGTCCCCCTG GTCCCCCTGG CTCTGCATCT TCAGGCTCTG GTGGTTTTGG GCCACCTGGT
    CCTCCTGGGC AAAATGGGGC GCCAGGTCAG CCGGGACTTT CGGGTGTACC AGGTGCTGAT
    GGAAAACCAG GCTTACCTGG TCCAAAAGGA GAGAAGGGTG ATGCCGGTGA GCTGGGCCTT
    CCTGGACCGG TGGGAGAGAA GGGTGCCAAA GGTTCTTCTG GTCCCCCTGG CACCACTGGG
    ATTGGTGGGC CTGCTGGTCT TCCAGGACCA ATTGGACCTA TTGGACTTCC AGGACCACCT
    GGTCCTCCTG GACCAAGCTT TAATGTTGGT TTTGATGACA TGGAAGGTTC TGGTGTTCAC
    TTCAGTTCAG TACCTGGAGT AAGAGGACCA GTTGGAATCC CGGGGCCACC TGGTGTCCCA
    GGGCCACAGG GTCAGCCTGG ATTCCCAGGG CTTCCTGGTG AGAAAGGCAG TTTAGGAGCT
    CAGGGAAATG ATGGTCTTCC TGGTGCAGAT GGCTTCCCTG GACCACAGGG ACCAAAGGGC
    GACAAAGGAG AGCGAGGCGA CAGGGGTGAA CCTGGTCGAG ATGGACATGG TCATCCAGGC
    CAACCAGGCC CTCCTGGACC ACCTGGACAG ATTATTTATC GCTACTCTGA ACATTATGAT
    GAAGTTGGAG GAACGGGGGC TCAGGGTGGT TCTGGTGTTC CTGGCCAAGC AGGATTCCCA
    GGCCCAATGG GACCAAAGGG TGACAGAGGT GAGCCTGGTC TTCCAGGCTA TGACATTAAG
    GGGGAGAAAG GAGAACCTGG ACTTATTCTT GGACCTGATG GAAATCCACT TTACCTTGGT
    GGATTGACAG GACAGAAGGG TGAGAGAGGT ATTTCTGGAC CAGTCGGACC TCCTGGTCCA
    GCAGGACCTC CTGGTGCAAA AGGTGAATTC GGAATGCCAG GCAGACCTGG TCGTCCAGGT
    GTGAACGGAT ATAAAGGAGA GAAGGGAGAG CCAAGTTCAG GCTCTGGATA TGGTTACCCG
    GGTCCCCCTG GTCCCCCAGG ACCCCCTGGT CCCCCCGGAC CCGCTGTGCC CTTGGACAGA
    TTTGGCAGAT ATGAAGATTA TGCAAGGCTC TATCCAGCTA CGAAAGGAGA GAAAGGAGAC
    CAAGGAGCTC CTGGCATTCC CGGATCGCCA GGTTTTTCCA CAAACTTTGA TATATATTCA
    CTCAAGAATG AGATGAAGGG TGAGCGGGGA GAGTCTGGTC TTAAAGGAGA GAAAGGAGAA
    CCAGGAGGTG GATTTTATGA CCCTCGTTTT GGAGCTGTAC AAGGACCACC AGGGAACCCA
    GGACCTGCTG GACCTAAAGG AGACTCAATC AGAGGCCCAC CTGGACCCCA GGGCCCACCA
    GGAACACCCG GGGTTGGTTA TGATGGTCGA CCAGGTAATC CAGGACCTCC TGGACCACCT
    GGTCCACCAG GGTCCCCATC ACTGCCAGGA CCCTATAGGC CACCACTCAG TATTCCTGGA
    CCTCCAGGTC CTCCTGGCCC ACCTGGAATT CCTGGCACTG GATCAGGGGT GACTGTCCTG
    AGGTCTCATG ACATAATGAT TGCTACAGCA CGCAGACAGA CAGAAGGGAC TCTGATTTAC
    ATTCTGGACA GAAACGATCT CTACCTCAGA GTTCGGGACG GTGTTAGACA AGTGATGCTT
    GGGGATTATA AACCTCTTTA TGGAGAGCTG GACAATGAAG TGGCAGCAGT CCAGCCACCC
    CCTGTTGTTC ACTATTCCCA GGACCACACG GCTAACAATG GCGCAGAGCA GATTTCTCCA
    CCACACCAGC CTATTGATTT CCCAACGAGA GAGCAGGAGA ACAGAAACCC CAACCCGCAA
    CCTGACCCCC GATACCCGGA TCCACGATAC CCACCATACA CTGACAACAG ATACACCGAT
    CCTGTAGAGC CCAACATCAG ATATCCTGTT CAACCCGAGA GGAATCCAAT CACTCCAGCC
    CGCCGTCCTA TCCCTCCTGT CAATCAGCCA GGAGGCCACG CCCACACCCA CACTTCCGGA
    CCAGGGTTGC ATCTTATTGC TCTGAATTCT CCACAAGTGG GCAACATGAG AGGCATTCGC
    GGGGCAGACT TCATGTGCTT CCAGCAGGCA CGGGCCGTTG GCATGAAGGG AACGTTCAGA
    GCCTTCCTGT CCTCCAAACT CCAGGATCTC TACAGTATCG TCCGCAGATC TGACAGAGAG
    ACATTACCAA TTGTTAACCT AAAGGATCAA GTCCTCTTCA GCAGCTGGGA GTCTCTCTTC
    AGTGACTCAG AGAGCAGGAT GAAGGACAAT GCACCCATCT ACTCGTTTGA TGGCAGAGAT
    GTCCTGAGGG ACAGTGCCTG GCCAGAGAAG ATGATTTGGC ACGGATCGGA TGGCAGGGGT
    CACAGGCAAA CGGATAACTA CTGCGAGACA TGGAGGGCCG GAGACCGCGC TGTCACAGGC
    CTGGCCTCGT CCCTGCAGGC TGGTCAACTC CTCCAGCAGA CCTCCAGCAG CTGCTCCAGC
    TCCTACATCG TGCTGTGCAT CGAGAACAGC TACATGACAC AGTCCAAGAA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa143964
    Clone ID Related Accession (Same CDS sequence) XM_021478722.1
    Accession Version XM_021478722.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4050bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product collagen type XVIII, alpha 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCAAGAA GGTGTCTCGC GGTTCTGGAG CGGTTCTTCT GCCTCGTGTT GATCTTGTCT 
    CCGGTTGCAT TACAGCAGAG AGAAGAAAGT GGAGTGTCGC TGTTGCAACT AATTGGCGAT
    CCTCCACCCA ACGGCGTCTC CAAAGTCTTC GACCAAGCAA ACAGTCCTGG ATATGTGTTT
    GGACCAAGCT CAAATGTGGG TCAGTCTGCT GCAGCCCACC TGCCCAATCC CTTCTACCGC
    GATTTCTCCC TCATCTTCAA TATAAAACCC ACATCCTCCA AGCCTGGCGT GATCTTCTCC
    ATCACTGACC CCACTCAGAA CATCATGTAT GTTGGCGTGA AGCTGTCGGC GGTGGAAAGA
    GGCAAACAGT CCATTATCTT CTACTACACC GAGCCTGATT CGCAGAGCTC CTATGAGGCG
    GCCAGATTCT CTGTTTCCTC CATGTTGAAC ATATGGACGC GCTTTTCCAT CAGCGTTTTG
    AACGAGCAGG TTTCTCTCTA CTTTAACTGC GACTCGGATC CTCAGGTTGT CAATTTCGAG
    CGCTCGCCTG ATGATATGGA CATGGATGCT GGCGCTGGAG TGTTTGTGGG ACATGCCAGT
    GGAGCAGACC CGGATAAGTT TTTGGGTGTT ATTGGTGATC TGAGGGTGTC GAGAGACCCT
    GGGGCGGCTG AACGGCACTG TGAAGAAGAG GAGGATGACT ATGCAACAGA GGCATCAGGT
    GATTACGGAG CAAGTGGAGA TGGAGAGCAG CCATCACCAG TTCAGCCAAC ACCTCCATCA
    TCTCGGCCAA TCCAGCGGCC ACCGGTGATT AGCAGGGATC CTATACAGCG GCCACCGGTG
    ATTAGCAGGG AGCCTATACA GCAGACAGTT GCAAGAGGAG AGAAGGGTGA CAGAGGGGAG
    AAGGGTGCTA AAGGTGACAG GGGTCTTGCT GGGCCAAAGG GTGATGTGGG GTCTGGATCA
    GTGTCTGGTG GAGGATCAAA AGGAGACAAG GGCGTCCCAG GAGAGAAAGG AATGAAGGGC
    ACTTCTGGTT TTGGTTATCC TGGATCTAAG GGTGACCGTG GCCCCCCTGG ACCTCCAGGA
    CCTCCGGGTC CCCAAGGGCC CAGTGCAGAG GTAGAGGTGC GGGGCGATGG GTCAGTTGTG
    CAGAAAGTCA CAGGACCAAG AGGACCACCT GGACCCCAAG GACCCCCTGG TCCCCCTGGA
    CCTGAAGGGG AGCCGGGTGA CCCTGGTGAA GATGGAAAAG CTGGCCAAGT TGGACCTTCT
    GGTTTTCCTG GAAATCCTGG GAATCCTGGA CCCAAAGGAG ATAAGGGAGA TCGAGGTGAA
    AGTCAGCCAG GACCCCGTGG GCCGCCTGGT CCTCCCGGCC CACCAGGACC CTCCTCTGGC
    TTTGATAGAC CTACGTTTGT GGACATGGAG GGCTCTGGAT TTGATATGGA CAGTGTGCGG
    GCAGTGCCTG GTTTGCCTGG TCCACCTGGT CCTCCTGGTC CCCCTGGTCC CCCTGGCTCT
    GCATCTTCAG GCTCTGGTGG TTTTGGGCCA CCTGGTCCTC CTGGGCAAAA TGGGGCGCCA
    GGTCAGCCGG GACTTTCGGG TGTACCAGGT GCTGATGGAA AACCAGGCTT ACCTGGTCCA
    AAAGGAGAGA AGGGTGATGC CGGTGAGCTG GGCCTTCCTG GACCGGTGGG AGAGAAGGGT
    GCCAAAGGTT CTTCTGGTCC CCCTGGCACC ACTGGGATTG GTGGGCCTGC TGGTCTTCCA
    GGACCAATTG GACCTATTGG ACTTCCAGGA CCACCTGGTC CTCCTGGACC AAGCTTTAAT
    GTTGGTTTTG ATGACATGGA AGGTTCTGGT GTTCACTTCA GTTCAGTACC TGGAGTAAGA
    GGACCAGTTG GAATCCCGGG GCCACCTGGT GTCCCAGGGC CACAGGGTCA GCCTGGATTC
    CCAGGGCTTC CTGGTGAGAA AGGCAGTTTA GGAGCTCAGG GAAATGATGG TCTTCCTGGT
    GCAGATGGCT TCCCTGGACC ACAGGGACCA AAGGGCGACA AAGGAGAGCG AGGCGACAGG
    GGTGAACCTG GTCGAGATGG ACATGGTCAT CCAGGCCAAC CAGGCCCTCC TGGACCACCT
    GGACAGATTA TTTATCGCTA CTCTGAACAT TATGATGAAG TTGGAGGAAC GGGGGCTCAG
    GGTGGTTCTG GTGTTCCTGG CCAAGCAGGA TTCCCAGGCC CAATGGGACC AAAGGGTGAC
    AGAGGTGAGC CTGGTCTTCC AGGCTATGAC ATTAAGGGGG AGAAAGGAGA ACCTGGACTT
    ATTCTTGGAC CTGATGGAAA TCCACTTTAC CTTGGTGGAT TGACAGGACA GAAGGGTGAG
    AGAGGTATTT CTGGACCAGT CGGACCTCCT GGTCCAGCAG GACCTCCTGG TGCAAAAGGT
    GAATTCGGAA TGCCAGGCAG ACCTGGTCGT CCAGGTGTGA ACGGATATAA AGGAGAGAAG
    GGAGAGCCAA GTTCAGGCTC TGGATATGGT TACCCGGGTC CCCCTGGTCC CCCAGGACCC
    CCTGGTCCCC CCGGACCCGC TGTGCCCTTG GACAGATTTG GCAGATATGA AGATTATGCA
    AGGCTCTATC CAGCTACGAA AGGAGAGAAA GGAGACCAAG GAGCTCCTGG CATTCCCGGA
    TCGCCAGGTT TTTCCACAAA CTTTGATATA TATTCACTCA AGAATGAGAT GAAGGGTGAG
    CGGGGAGAGT CTGGTCTTAA AGGAGAGAAA GGAGAACCAG GAGGTGGATT TTATGACCCT
    CGTTTTGGAG CTGTACAAGG ACCACCAGGG AACCCAGGAC CTGCTGGACC TAAAGGAGAC
    TCAATCAGAG GCCCACCTGG ACCCCAGGGC CCACCAGGAA CACCCGGGGT TGGTTATGAT
    GGTCGACCAG GTAATCCAGG ACCTCCTGGA CCACCTGGTC CACCAGGGTC CCCATCACTG
    CCAGGACCCT ATAGGCCACC ACTCAGTATT CCTGGACCTC CAGGTCCTCC TGGCCCACCT
    GGAATTCCTG GCACTGGATC AGGGGTGACT GTCCTGAGGT CTCATGACAT AATGATTGCT
    ACAGCACGCA GACAGACAGA AGGGACTCTG ATTTACATTC TGGACAGAAA CGATCTCTAC
    CTCAGAGTTC GGGACGGTGT TAGACAAGTG ATGCTTGGGG ATTATAAACC TCTTTATGGA
    GAGCTGGACA ATGAAGTGGC AGCAGTCCAG CCACCCCCTG TTGTTCACTA TTCCCAGGAC
    CACACGGCTA ACAATGGCGC AGAGCAGATT TCTCCACCAC ACCAGCCTAT TGATTTCCCA
    ACGAGAGAGC AGGAGAACAG AAACCCCAAC CCGCAACCTG ACCCCCGATA CCCGGATCCA
    CGATACCCAC CATACACTGA CAACAGATAC ACCGATCCTG TAGAGCCCAA CATCAGATAT
    CCTGTTCAAC CCGAGAGGAA TCCAATCACT CCAGCCCGCC GTCCTATCCC TCCTGTCAAT
    CAGCCAGGAG GCCACGCCCA CACCCACACT TCCGGACCAG GGTTGCATCT TATTGCTCTG
    AATTCTCCAC AAGTGGGCAA CATGAGAGGC ATTCGCGGGG CAGACTTCAT GTGCTTCCAG
    CAGGCACGGG CCGTTGGCAT GAAGGGAACG TTCAGAGCCT TCCTGTCCTC CAAACTCCAG
    GATCTCTACA GTATCGTCCG CAGATCTGAC AGAGAGACAT TACCAATTGT TAACCTAAAG
    GATCAAGTCC TCTTCAGCAG CTGGGAGTCT CTCTTCAGTG ACTCAGAGAG CAGGATGAAG
    GACAATGCAC CCATCTACTC GTTTGATGGC AGAGATGTCC TGAGGGACAG TGCCTGGCCA
    GAGAAGATGA TTTGGCACGG ATCGGATGGC AGGGGTCACA GGCAAACGGA TAACTACTGC
    GAGACATGGA GGGCCGGAGA CCGCGCTGTC ACAGGCCTGG CCTCGTCCCT GCAGGCTGGT
    CAACTCCTCC AGCAGACCTC CAGCAGCTGC TCCAGCTCCT ACATCGTGCT GTGCATCGAG
    AACAGCTACA TGACACAGTC CAAGAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021334397.1
    CDS178..4227
    Translation

    Target ORF information:

    RefSeq Version XM_021478722.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio collagen type XVIII alpha 1 chain a (col18a1a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021478722.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCAAGAA GGTGTCTCGC GGTTCTGGAG CGGTTCTTCT GCCTCGTGTT GATCTTGTCT 
    CCGGTTGCAT TACAGCAGAG AGAAGAAAGT GGAGTGTCGC TGTTGCAACT AATTGGCGAT
    CCTCCACCCA ACGGCGTCTC CAAAGTCTTC GACCAAGCAA ACAGTCCTGG ATATGTGTTT
    GGACCAAGCT CAAATGTGGG TCAGTCTGCT GCAGCCCACC TGCCCAATCC CTTCTACCGC
    GATTTCTCCC TCATCTTCAA TATAAAACCC ACATCCTCCA AGCCTGGCGT GATCTTCTCC
    ATCACTGACC CCACTCAGAA CATCATGTAT GTTGGCGTGA AGCTGTCGGC GGTGGAAAGA
    GGCAAACAGT CCATTATCTT CTACTACACC GAGCCTGATT CGCAGAGCTC CTATGAGGCG
    GCCAGATTCT CTGTTTCCTC CATGTTGAAC ATATGGACGC GCTTTTCCAT CAGCGTTTTG
    AACGAGCAGG TTTCTCTCTA CTTTAACTGC GACTCGGATC CTCAGGTTGT CAATTTCGAG
    CGCTCGCCTG ATGATATGGA CATGGATGCT GGCGCTGGAG TGTTTGTGGG ACATGCCAGT
    GGAGCAGACC CGGATAAGTT TTTGGGTGTT ATTGGTGATC TGAGGGTGTC GAGAGACCCT
    GGGGCGGCTG AACGGCACTG TGAAGAAGAG GAGGATGACT ATGCAACAGA GGCATCAGGT
    GATTACGGAG CAAGTGGAGA TGGAGAGCAG CCATCACCAG TTCAGCCAAC ACCTCCATCA
    TCTCGGCCAA TCCAGCGGCC ACCGGTGATT AGCAGGGATC CTATACAGCG GCCACCGGTG
    ATTAGCAGGG AGCCTATACA GCAGACAGTT GCAAGAGGAG AGAAGGGTGA CAGAGGGGAG
    AAGGGTGCTA AAGGTGACAG GGGTCTTGCT GGGCCAAAGG GTGATGTGGG GTCTGGATCA
    GTGTCTGGTG GAGGATCAAA AGGAGACAAG GGCGTCCCAG GAGAGAAAGG AATGAAGGGC
    ACTTCTGGTT TTGGTTATCC TGGATCTAAG GGTGACCGTG GCCCCCCTGG ACCTCCAGGA
    CCTCCGGGTC CCCAAGGGCC CAGTGCAGAG GTAGAGGTGC GGGGCGATGG GTCAGTTGTG
    CAGAAAGTCA CAGGACCAAG AGGACCACCT GGACCCCAAG GACCCCCTGG TCCCCCTGGA
    CCTGAAGGGG AGCCGGGTGA CCCTGGTGAA GATGGAAAAG CTGGCCAAGT TGGACCTTCT
    GGTTTTCCTG GAAATCCTGG GAATCCTGGA CCCAAAGGAG ATAAGGGAGA TCGAGGTGAA
    AGTCAGCCAG GACCCCGTGG GCCGCCTGGT CCTCCCGGCC CACCAGGACC CTCCTCTGGC
    TTTGATAGAC CTACGTTTGT GGACATGGAG GGCTCTGGAT TTGATATGGA CAGTGTGCGG
    GCAGTGCCTG GTTTGCCTGG TCCACCTGGT CCTCCTGGTC CCCCTGGTCC CCCTGGCTCT
    GCATCTTCAG GCTCTGGTGG TTTTGGGCCA CCTGGTCCTC CTGGGCAAAA TGGGGCGCCA
    GGTCAGCCGG GACTTTCGGG TGTACCAGGT GCTGATGGAA AACCAGGCTT ACCTGGTCCA
    AAAGGAGAGA AGGGTGATGC CGGTGAGCTG GGCCTTCCTG GACCGGTGGG AGAGAAGGGT
    GCCAAAGGTT CTTCTGGTCC CCCTGGCACC ACTGGGATTG GTGGGCCTGC TGGTCTTCCA
    GGACCAATTG GACCTATTGG ACTTCCAGGA CCACCTGGTC CTCCTGGACC AAGCTTTAAT
    GTTGGTTTTG ATGACATGGA AGGTTCTGGT GTTCACTTCA GTTCAGTACC TGGAGTAAGA
    GGACCAGTTG GAATCCCGGG GCCACCTGGT GTCCCAGGGC CACAGGGTCA GCCTGGATTC
    CCAGGGCTTC CTGGTGAGAA AGGCAGTTTA GGAGCTCAGG GAAATGATGG TCTTCCTGGT
    GCAGATGGCT TCCCTGGACC ACAGGGACCA AAGGGCGACA AAGGAGAGCG AGGCGACAGG
    GGTGAACCTG GTCGAGATGG ACATGGTCAT CCAGGCCAAC CAGGCCCTCC TGGACCACCT
    GGACAGATTA TTTATCGCTA CTCTGAACAT TATGATGAAG TTGGAGGAAC GGGGGCTCAG
    GGTGGTTCTG GTGTTCCTGG CCAAGCAGGA TTCCCAGGCC CAATGGGACC AAAGGGTGAC
    AGAGGTGAGC CTGGTCTTCC AGGCTATGAC ATTAAGGGGG AGAAAGGAGA ACCTGGACTT
    ATTCTTGGAC CTGATGGAAA TCCACTTTAC CTTGGTGGAT TGACAGGACA GAAGGGTGAG
    AGAGGTATTT CTGGACCAGT CGGACCTCCT GGTCCAGCAG GACCTCCTGG TGCAAAAGGT
    GAATTCGGAA TGCCAGGCAG ACCTGGTCGT CCAGGTGTGA ACGGATATAA AGGAGAGAAG
    GGAGAGCCAA GTTCAGGCTC TGGATATGGT TACCCGGGTC CCCCTGGTCC CCCAGGACCC
    CCTGGTCCCC CCGGACCCGC TGTGCCCTTG GACAGATTTG GCAGATATGA AGATTATGCA
    AGGCTCTATC CAGCTACGAA AGGAGAGAAA GGAGACCAAG GAGCTCCTGG CATTCCCGGA
    TCGCCAGGTT TTTCCACAAA CTTTGATATA TATTCACTCA AGAATGAGAT GAAGGGTGAG
    CGGGGAGAGT CTGGTCTTAA AGGAGAGAAA GGAGAACCAG GAGGTGGATT TTATGACCCT
    CGTTTTGGAG CTGTACAAGG ACCACCAGGG AACCCAGGAC CTGCTGGACC TAAAGGAGAC
    TCAATCAGAG GCCCACCTGG ACCCCAGGGC CCACCAGGAA CACCCGGGGT TGGTTATGAT
    GGTCGACCAG GTAATCCAGG ACCTCCTGGA CCACCTGGTC CACCAGGGTC CCCATCACTG
    CCAGGACCCT ATAGGCCACC ACTCAGTATT CCTGGACCTC CAGGTCCTCC TGGCCCACCT
    GGAATTCCTG GCACTGGATC AGGGGTGACT GTCCTGAGGT CTCATGACAT AATGATTGCT
    ACAGCACGCA GACAGACAGA AGGGACTCTG ATTTACATTC TGGACAGAAA CGATCTCTAC
    CTCAGAGTTC GGGACGGTGT TAGACAAGTG ATGCTTGGGG ATTATAAACC TCTTTATGGA
    GAGCTGGACA ATGAAGTGGC AGCAGTCCAG CCACCCCCTG TTGTTCACTA TTCCCAGGAC
    CACACGGCTA ACAATGGCGC AGAGCAGATT TCTCCACCAC ACCAGCCTAT TGATTTCCCA
    ACGAGAGAGC AGGAGAACAG AAACCCCAAC CCGCAACCTG ACCCCCGATA CCCGGATCCA
    CGATACCCAC CATACACTGA CAACAGATAC ACCGATCCTG TAGAGCCCAA CATCAGATAT
    CCTGTTCAAC CCGAGAGGAA TCCAATCACT CCAGCCCGCC GTCCTATCCC TCCTGTCAAT
    CAGCCAGGAG GCCACGCCCA CACCCACACT TCCGGACCAG GGTTGCATCT TATTGCTCTG
    AATTCTCCAC AAGTGGGCAA CATGAGAGGC ATTCGCGGGG CAGACTTCAT GTGCTTCCAG
    CAGGCACGGG CCGTTGGCAT GAAGGGAACG TTCAGAGCCT TCCTGTCCTC CAAACTCCAG
    GATCTCTACA GTATCGTCCG CAGATCTGAC AGAGAGACAT TACCAATTGT TAACCTAAAG
    GATCAAGTCC TCTTCAGCAG CTGGGAGTCT CTCTTCAGTG ACTCAGAGAG CAGGATGAAG
    GACAATGCAC CCATCTACTC GTTTGATGGC AGAGATGTCC TGAGGGACAG TGCCTGGCCA
    GAGAAGATGA TTTGGCACGG ATCGGATGGC AGGGGTCACA GGCAAACGGA TAACTACTGC
    GAGACATGGA GGGCCGGAGA CCGCGCTGTC ACAGGCCTGG CCTCGTCCCT GCAGGCTGGT
    CAACTCCTCC AGCAGACCTC CAGCAGCTGC TCCAGCTCCT ACATCGTGCT GTGCATCGAG
    AACAGCTACA TGACACAGTC CAAGAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.