cacna1aa cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	cacna1aa
Entrez Gene ID	562059
Full Name	calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a
Synonyms	cacna1a,si:ch211-237k17.1
General protein information	Preferred Names calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a Names voltage-dependent P/Q-type calcium channel subunit alpha-1A ZfCav2.1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021474379.1	XP_021330054.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X3
XM_021474393.1	XP_021330068.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X7
XM_021474404.1	XP_021330079.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X11
XM_009299585.3	XP_009297860.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X14
XM_009299590.3	XP_009297865.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X20
XM_021474433.1	XP_021330108.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X23
XM_005164090.4	XP_005164147.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X24
XM_021474440.1	XP_021330115.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X25
XM_017354795.1	XP_017210284.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X6
XM_009299587.2	XP_009297862.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X12
XM_017354799.1	XP_017210288.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X11
XM_017354791.1	XP_017210280.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X2
XM_017354796.1	XP_017210285.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X7
XM_009299586.2	XP_009297861.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X10
XM_009299590.2	XP_009297865.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X16
XM_009299591.2	XP_009297866.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X17
XM_009299592.2	XP_009297867.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X20
XM_021474381.1	XP_021330056.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X4
XM_021474390.1	XP_021330065.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X6
XM_021474403.1	XP_021330078.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X10
XM_017354794.2	XP_017210283.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X15
XM_021474420.1	XP_021330095.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X16
XM_021474423.1	XP_021330098.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X18
XM_017354793.1	XP_017210282.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X4
XM_017354794.1	XP_017210283.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X5
XM_017354800.1	XP_017210289.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X15
XM_017354801.1	XP_017210290.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X18
XM_021474398.1	XP_021330073.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X9
XM_021474408.1	XP_021330083.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X12
XM_021474412.1	XP_021330087.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X13
XM_009299591.3	XP_009297866.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X21
XM_021474432.1	XP_021330107.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X22
XM_017354792.1	XP_017210281.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X3
XM_017354797.1	XP_017210286.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X8
XM_009299588.2	XP_009297863.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X13
XM_021474394.1	XP_021330069.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X8
XM_021474442.1	XP_021330117.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X28
XM_017354798.1	XP_017210287.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X9
XM_009299585.2	XP_009297860.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X1
XM_009299589.2	XP_009297864.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X14
XM_005164090.3	XP_005164147.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X19
XM_009299593.2	XP_009297868.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X21
XM_021474372.1	XP_021330047.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X1
XM_021474375.1	XP_021330050.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X2
XM_021474386.1	XP_021330061.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X5
XM_021474422.1	XP_021330097.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X17
XM_021474428.1	XP_021330103.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X19
XM_009299593.3	XP_009297868.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X26
XM_021474441.1	XP_021330116.1	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X27

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002577	XP_563586.4
Danio rerio (zebrafish)	cacna1aa	XP_690548.6

Gene Ontology

The following cacna1aa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cacna1aa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa08534	XM_005164090.4 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa141783	XM_021474372.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa141784	XM_021474375.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa141785	XM_021474379.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa141786	XM_021474381.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa141787	XM_021474386.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa141788	XM_021474390.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa141789	XM_021474393.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa141790	XM_021474394.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa141791	XM_021474398.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa141792	XM_021474403.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa141793	XM_021474404.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa141794	XM_021474408.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa141795	XM_021474412.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa141796	XM_021474420.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa141797	XM_021474422.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa141798	XM_021474423.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa141799	XM_021474428.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa141800	XM_021474432.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa141801	XM_021474433.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa141802	XM_021474440.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
ODa141803	XM_021474441.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa141804	XM_021474442.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa43660	XM_009299585.3 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa43661	XM_009299586.2 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa43662	XM_009299587.2 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa43663	XM_009299588.2 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa43664	XM_009299589.2 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa43665	XM_009299590.3 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa43666	XM_009299591.3 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa43667	XM_009299592.2 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
ODa43668	XM_009299593.3 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa58247	XM_017354791.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa58248	XM_017354792.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa58249	XM_017354793.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa58250	XM_017354794.2 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa58251	XM_017354795.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa58252	XM_017354796.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa58253	XM_017354797.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa58254	XM_017354798.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa58255	XM_017354799.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa58256	XM_017354800.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa58257	XM_017354801.1 Latest version!	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
View Old Accession Versions >>
ODa43665	XM_009299590.2	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa58250	XM_017354794.1	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa43666	XM_009299591.2	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa43660	XM_009299585.2	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa08534	XM_005164090.3	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa43668	XM_009299593.2	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cacna1aa ( XM_005164090.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164147.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X24, mRNA.

Price & Availability↑

CloneID	ODa08534
Clone ID Related Accession (Same CDS sequence)	XM_005164090.4 , XM_005164090.3
Accession Version	XM_005164090.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005164090.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164147.1
CDS	1542..6764
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGGSGGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGMNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAA

Target ORF information:

RefSeq Version	XM_005164090.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164090.4

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_005164090.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164147.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X19, mRNA.

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CloneID	ODa08534
Clone ID Related Accession (Same CDS sequence)	XM_005164090.4 , XM_005164090.3
Accession Version	XM_005164090.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X19
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005164090.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164147.1
CDS	1507..6729
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGGSGGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGMNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAA

Target ORF information:

RefSeq Version	XM_005164090.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164090.3

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_021474372.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330047.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X1, mRNA.

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CloneID	ODa141783
Clone ID Related Accession (Same CDS sequence)	XM_021474372.1
Accession Version	XM_021474372.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG 
GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT 
GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC 
CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC 
ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT 
GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT 
GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT 
GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT 
CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA 
AGGCAGTCTC AGACAGTAGA GATGAGAGAG ATGGGGCGGG ACGGGTACTC GGACACAGAG 
CAATATCTGC CGATGGAAGG CCATGGCAGG GCTGCTTCCA TGCCTCGACT CCCAGCAGAC 
AACCAACAGC CACGGAGGAA AGGGCGTCAC CGGGGAGAAA ATCTCACTCA GCCTATCACA 
GATAACAGTC CTATGAAGCG CTCGGCTTCA TCTTTGGGAC ACGGGCGTGC AGGACGCTCC 
ATGCGAGGTG AGGATTATGC CATGGAGAGG GTTATCCCAG AAGAGGGTCA CAGGCATGGG 
CATCGCCACC GAGACCGTAG TCACCGGGCG TCAGAACGCT CCCTGAGCCG ATACACTGAT 
GCTGACACAG GTCTTGGAAC TGACCTCAGC ACAACCACAC AATCAGGAGA CCTGCCATCC 
AAGGAGAGAG ACCGGGGCCG AGCCAAAGAC CGCAAGCACC ATCATCATCA TCATCACCAC 
CACGGCTCTT TAGATAAGGA GCACTACGGT CATGAGAGAG ACAGAGGAGA GTACGGCCAC 
AGACAGTCCA GAGAGAGAGA CCGCCGCTGG TCCCGCTCAC CCAGTGAGGG CAGAGAGTGT 
CTGACTCACA GACAGGGCAG TAGTTCAGTA AGCGGCAGTC CAGTCCCGTC CACCTCCGGG 
ACCAGCACCC CCCGCCGTGG CCGCCGTCAG CTCCCCCAAA CCCCTGCTAC ACCCCGGCCA 
CATGTCACCT ACTCACCTGT GGTCCGTAAG CCCATCAGCA GTACCCCCCC TCCTGGCCAG 
CAGAGCCGAC TCCCTACCCC GACCTCCCGG CGCTTCAGTC CGACTGGCCC CGAGCCCCCG 
CTTCCCCCTC ATCACCACCC GTCACCACCC CACCACGGCT CTCCCCGCTC CGGCCGACAT 
GCACACTGGG GCCCCGAGCC TGCAGAGAGC GTGGAGGGTG ACGGCTTCTA TGACGACCAA 
GACTATGAGT TCAATCACCA CGAGCCTCCA TCCTACGAGC AAAGCCCTGT GCAGGGCAAC 
CCTCACCCCC ATTCCCCTAG GACTTCCCGC CACAACACCC CTCCTCAGGG CCAAGCGCAC 
CCACGGCGCA TGCCAAATGG CTACCGTTCG TCTTCGCCCT CTCCACACCA CCACGCGCCC 
GCTCACCCTG GAACTCATAA ACCTCCTCAT CCCAGAGGGC CCAGAAAGGG CCTCCATGAA 
CCCTACAGTG AGACTGACGA GGATGACTGG TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330047.1
CDS	1507..8622
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGGSGGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGMNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGEGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQNRTPLMFQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQTVEMREMGRDGYSDTEQYLPMEGHGRAASMPRLPADNQQPRRKGRHRGENLTQPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_021474372.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474372.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG 
GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT 
GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC 
CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC 
ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT 
GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT 
GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT 
GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT 
CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA 
AGGCAGTCTC AGACAGTAGA GATGAGAGAG ATGGGGCGGG ACGGGTACTC GGACACAGAG 
CAATATCTGC CGATGGAAGG CCATGGCAGG GCTGCTTCCA TGCCTCGACT CCCAGCAGAC 
AACCAACAGC CACGGAGGAA AGGGCGTCAC CGGGGAGAAA ATCTCACTCA GCCTATCACA 
GATAACAGTC CTATGAAGCG CTCGGCTTCA TCTTTGGGAC ACGGGCGTGC AGGACGCTCC 
ATGCGAGGTG AGGATTATGC CATGGAGAGG GTTATCCCAG AAGAGGGTCA CAGGCATGGG 
CATCGCCACC GAGACCGTAG TCACCGGGCG TCAGAACGCT CCCTGAGCCG ATACACTGAT 
GCTGACACAG GTCTTGGAAC TGACCTCAGC ACAACCACAC AATCAGGAGA CCTGCCATCC 
AAGGAGAGAG ACCGGGGCCG AGCCAAAGAC CGCAAGCACC ATCATCATCA TCATCACCAC 
CACGGCTCTT TAGATAAGGA GCACTACGGT CATGAGAGAG ACAGAGGAGA GTACGGCCAC 
AGACAGTCCA GAGAGAGAGA CCGCCGCTGG TCCCGCTCAC CCAGTGAGGG CAGAGAGTGT 
CTGACTCACA GACAGGGCAG TAGTTCAGTA AGCGGCAGTC CAGTCCCGTC CACCTCCGGG 
ACCAGCACCC CCCGCCGTGG CCGCCGTCAG CTCCCCCAAA CCCCTGCTAC ACCCCGGCCA 
CATGTCACCT ACTCACCTGT GGTCCGTAAG CCCATCAGCA GTACCCCCCC TCCTGGCCAG 
CAGAGCCGAC TCCCTACCCC GACCTCCCGG CGCTTCAGTC CGACTGGCCC CGAGCCCCCG 
CTTCCCCCTC ATCACCACCC GTCACCACCC CACCACGGCT CTCCCCGCTC CGGCCGACAT 
GCACACTGGG GCCCCGAGCC TGCAGAGAGC GTGGAGGGTG ACGGCTTCTA TGACGACCAA 
GACTATGAGT TCAATCACCA CGAGCCTCCA TCCTACGAGC AAAGCCCTGT GCAGGGCAAC 
CCTCACCCCC ATTCCCCTAG GACTTCCCGC CACAACACCC CTCCTCAGGG CCAAGCGCAC 
CCACGGCGCA TGCCAAATGG CTACCGTTCG TCTTCGCCCT CTCCACACCA CCACGCGCCC 
GCTCACCCTG GAACTCATAA ACCTCCTCAT CCCAGAGGGC CCAGAAAGGG CCTCCATGAA 
CCCTACAGTG AGACTGACGA GGATGACTGG TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_021474375.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330050.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X2, mRNA.

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CloneID	ODa141784
Clone ID Related Accession (Same CDS sequence)	XM_021474375.1
Accession Version	XM_021474375.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGTGGCAGAA TCAGTTACAG GGATATGTAT GAGATGCTCA GGCACATGTC TCCTCCGTTA 
GGCCTGGGGA AGAAGTGCCC TGCACGGGTT GCCTACAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT 
GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC 
CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC 
ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT 
GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT 
GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT 
GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT 
CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA 
AGGCAGTCTC AGACAGTAGA GATGAGAGAG ATGGGGCGGG ACGGGTACTC GGACACAGAG 
CAATATCTGC CGATGGAAGG CCATGGCAGG GCTGCTTCCA TGCCTCGACT CCCAGCAGAC 
AACCAACAGC CACGGAGGAA AGGGCGTCAC CGGGGAGAAA ATCTCACTCA GCCTATCACA 
GATAACAGTC CTATGAAGCG CTCGGCTTCA TCTTTGGGAC ACGGGCGTGC AGGACGCTCC 
ATGCGAGGTG AGGATTATGC CATGGAGAGG GTTATCCCAG AAGAGGGTCA CAGGCATGGG 
CATCGCCACC GAGACCGTAG TCACCGGGCG TCAGAACGCT CCCTGAGCCG ATACACTGAT 
GCTGACACAG GTCTTGGAAC TGACCTCAGC ACAACCACAC AATCAGGAGA CCTGCCATCC 
AAGGAGAGAG ACCGGGGCCG AGCCAAAGAC CGCAAGCACC ATCATCATCA TCATCACCAC 
CACGGCTCTT TAGATAAGGA GCACTACGGT CATGAGAGAG ACAGAGGAGA GTACGGCCAC 
AGACAGTCCA GAGAGAGAGA CCGCCGCTGG TCCCGCTCAC CCAGTGAGGG CAGAGAGTGT 
CTGACTCACA GACAGGGCAG TAGTTCAGTA AGCGGCAGTC CAGTCCCGTC CACCTCCGGG 
ACCAGCACCC CCCGCCGTGG CCGCCGTCAG CTCCCCCAAA CCCCTGCTAC ACCCCGGCCA 
CATGTCACCT ACTCACCTGT GGTCCGTAAG CCCATCAGCA GTACCCCCCC TCCTGGCCAG 
CAGAGCCGAC TCCCTACCCC GACCTCCCGG CGCTTCAGTC CGACTGGCCC CGAGCCCCCG 
CTTCCCCCTC ATCACCACCC GTCACCACCC CACCACGGCT CTCCCCGCTC CGGCCGACAT 
GCACACTGGG GCCCCGAGCC TGCAGAGAGC GTGGAGGGTG ACGGCTTCTA TGACGACCAA 
GACTATGAGT TCAATCACCA CGAGCCTCCA TCCTACGAGC AAAGCCCTGT GCAGGGCAAC 
CCTCACCCCC ATTCCCCTAG GACTTCCCGC CACAACACCC CTCCTCAGGG CCAAGCGCAC 
CCACGGCGCA TGCCAAATGG CTACCGTTCG TCTTCGCCCT CTCCACACCA CCACGCGCCC 
GCTCACCCTG GAACTCATAA ACCTCCTCAT CCCAGAGGGC CCAGAAAGGG CCTCCATGAA 
CCCTACAGTG AGACTGACGA GGATGACTGG TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330050.1
CDS	1508..8623
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGGSGGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGMNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRISYRDMYEMLRHMSPPLGLGKKCPARVAYKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGEGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQNRTPLMFQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQTVEMREMGRDGYSDTEQYLPMEGHGRAASMPRLPADNQQPRRKGRHRGENLTQPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_021474375.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474375.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGTGGCAGAA TCAGTTACAG GGATATGTAT GAGATGCTCA GGCACATGTC TCCTCCGTTA 
GGCCTGGGGA AGAAGTGCCC TGCACGGGTT GCCTACAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT 
GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC 
CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC 
ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT 
GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT 
GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT 
GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT 
CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA 
AGGCAGTCTC AGACAGTAGA GATGAGAGAG ATGGGGCGGG ACGGGTACTC GGACACAGAG 
CAATATCTGC CGATGGAAGG CCATGGCAGG GCTGCTTCCA TGCCTCGACT CCCAGCAGAC 
AACCAACAGC CACGGAGGAA AGGGCGTCAC CGGGGAGAAA ATCTCACTCA GCCTATCACA 
GATAACAGTC CTATGAAGCG CTCGGCTTCA TCTTTGGGAC ACGGGCGTGC AGGACGCTCC 
ATGCGAGGTG AGGATTATGC CATGGAGAGG GTTATCCCAG AAGAGGGTCA CAGGCATGGG 
CATCGCCACC GAGACCGTAG TCACCGGGCG TCAGAACGCT CCCTGAGCCG ATACACTGAT 
GCTGACACAG GTCTTGGAAC TGACCTCAGC ACAACCACAC AATCAGGAGA CCTGCCATCC 
AAGGAGAGAG ACCGGGGCCG AGCCAAAGAC CGCAAGCACC ATCATCATCA TCATCACCAC 
CACGGCTCTT TAGATAAGGA GCACTACGGT CATGAGAGAG ACAGAGGAGA GTACGGCCAC 
AGACAGTCCA GAGAGAGAGA CCGCCGCTGG TCCCGCTCAC CCAGTGAGGG CAGAGAGTGT 
CTGACTCACA GACAGGGCAG TAGTTCAGTA AGCGGCAGTC CAGTCCCGTC CACCTCCGGG 
ACCAGCACCC CCCGCCGTGG CCGCCGTCAG CTCCCCCAAA CCCCTGCTAC ACCCCGGCCA 
CATGTCACCT ACTCACCTGT GGTCCGTAAG CCCATCAGCA GTACCCCCCC TCCTGGCCAG 
CAGAGCCGAC TCCCTACCCC GACCTCCCGG CGCTTCAGTC CGACTGGCCC CGAGCCCCCG 
CTTCCCCCTC ATCACCACCC GTCACCACCC CACCACGGCT CTCCCCGCTC CGGCCGACAT 
GCACACTGGG GCCCCGAGCC TGCAGAGAGC GTGGAGGGTG ACGGCTTCTA TGACGACCAA 
GACTATGAGT TCAATCACCA CGAGCCTCCA TCCTACGAGC AAAGCCCTGT GCAGGGCAAC 
CCTCACCCCC ATTCCCCTAG GACTTCCCGC CACAACACCC CTCCTCAGGG CCAAGCGCAC 
CCACGGCGCA TGCCAAATGG CTACCGTTCG TCTTCGCCCT CTCCACACCA CCACGCGCCC 
GCTCACCCTG GAACTCATAA ACCTCCTCAT CCCAGAGGGC CCAGAAAGGG CCTCCATGAA 
CCCTACAGTG AGACTGACGA GGATGACTGG TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_021474379.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330054.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X3, mRNA.

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CloneID	ODa141785
Clone ID Related Accession (Same CDS sequence)	XM_021474379.1
Accession Version	XM_021474379.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG 
GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT 
GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC 
CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC 
ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT 
GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT 
GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT 
GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT 
CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA 
AGGCAGTCTC AGACAGTAGA GATGAGAGAG ATGGGGCGGG ACGGGTACTC GGACACAGAG 
CAATATCTGC CGATGGAAGG CCATGGCAGG GCTGCTTCCA TGCCTCGACT CCCAGCAGAC 
AACCAACAGC CACGGAGGAA AGGGCGTCAC CGGGGAGAAA ATCTCACTCC TATCACAGAT 
AACAGTCCTA TGAAGCGCTC GGCTTCATCT TTGGGACACG GGCGTGCAGG ACGCTCCATG 
CGAGGTGAGG ATTATGCCAT GGAGAGGGTT ATCCCAGAAG AGGGTCACAG GCATGGGCAT 
CGCCACCGAG ACCGTAGTCA CCGGGCGTCA GAACGCTCCC TGAGCCGATA CACTGATGCT 
GACACAGGTC TTGGAACTGA CCTCAGCACA ACCACACAAT CAGGAGACCT GCCATCCAAG 
GAGAGAGACC GGGGCCGAGC CAAAGACCGC AAGCACCATC ATCATCATCA TCACCACCAC 
GGCTCTTTAG ATAAGGAGCA CTACGGTCAT GAGAGAGACA GAGGAGAGTA CGGCCACAGA 
CAGTCCAGAG AGAGAGACCG CCGCTGGTCC CGCTCACCCA GTGAGGGCAG AGAGTGTCTG 
ACTCACAGAC AGGGCAGTAG TTCAGTAAGC GGCAGTCCAG TCCCGTCCAC CTCCGGGACC 
AGCACCCCCC GCCGTGGCCG CCGTCAGCTC CCCCAAACCC CTGCTACACC CCGGCCACAT 
GTCACCTACT CACCTGTGGT CCGTAAGCCC ATCAGCAGTA CCCCCCCTCC TGGCCAGCAG 
AGCCGACTCC CTACCCCGAC CTCCCGGCGC TTCAGTCCGA CTGGCCCCGA GCCCCCGCTT 
CCCCCTCATC ACCACCCGTC ACCACCCCAC CACGGCTCTC CCCGCTCCGG CCGACATGCA 
CACTGGGGCC CCGAGCCTGC AGAGAGCGTG GAGGGTGACG GCTTCTATGA CGACCAAGAC 
TATGAGTTCA ATCACCACGA GCCTCCATCC TACGAGCAAA GCCCTGTGCA GGGCAACCCT 
CACCCCCATT CCCCTAGGAC TTCCCGCCAC AACACCCCTC CTCAGGGCCA AGCGCACCCA 
CGGCGCATGC CAAATGGCTA CCGTTCGTCT TCGCCCTCTC CACACCACCA CGCGCCCGCT 
CACCCTGGAA CTCATAAACC TCCTCATCCC AGAGGGCCCA GAAAGGGCCT CCATGAACCC 
TACAGTGAGA CTGACGAGGA TGACTGGTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330054.1
CDS	1507..8619
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGGSGGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGMNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGEGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQNRTPLMFQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQTVEMREMGRDGYSDTEQYLPMEGHGRAASMPRLPADNQQPRRKGRHRGENLTPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_021474379.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474379.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG 
GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT 
GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC 
CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC 
ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT 
GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT 
GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT 
GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT 
CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA 
AGGCAGTCTC AGACAGTAGA GATGAGAGAG ATGGGGCGGG ACGGGTACTC GGACACAGAG 
CAATATCTGC CGATGGAAGG CCATGGCAGG GCTGCTTCCA TGCCTCGACT CCCAGCAGAC 
AACCAACAGC CACGGAGGAA AGGGCGTCAC CGGGGAGAAA ATCTCACTCC TATCACAGAT 
AACAGTCCTA TGAAGCGCTC GGCTTCATCT TTGGGACACG GGCGTGCAGG ACGCTCCATG 
CGAGGTGAGG ATTATGCCAT GGAGAGGGTT ATCCCAGAAG AGGGTCACAG GCATGGGCAT 
CGCCACCGAG ACCGTAGTCA CCGGGCGTCA GAACGCTCCC TGAGCCGATA CACTGATGCT 
GACACAGGTC TTGGAACTGA CCTCAGCACA ACCACACAAT CAGGAGACCT GCCATCCAAG 
GAGAGAGACC GGGGCCGAGC CAAAGACCGC AAGCACCATC ATCATCATCA TCACCACCAC 
GGCTCTTTAG ATAAGGAGCA CTACGGTCAT GAGAGAGACA GAGGAGAGTA CGGCCACAGA 
CAGTCCAGAG AGAGAGACCG CCGCTGGTCC CGCTCACCCA GTGAGGGCAG AGAGTGTCTG 
ACTCACAGAC AGGGCAGTAG TTCAGTAAGC GGCAGTCCAG TCCCGTCCAC CTCCGGGACC 
AGCACCCCCC GCCGTGGCCG CCGTCAGCTC CCCCAAACCC CTGCTACACC CCGGCCACAT 
GTCACCTACT CACCTGTGGT CCGTAAGCCC ATCAGCAGTA CCCCCCCTCC TGGCCAGCAG 
AGCCGACTCC CTACCCCGAC CTCCCGGCGC TTCAGTCCGA CTGGCCCCGA GCCCCCGCTT 
CCCCCTCATC ACCACCCGTC ACCACCCCAC CACGGCTCTC CCCGCTCCGG CCGACATGCA 
CACTGGGGCC CCGAGCCTGC AGAGAGCGTG GAGGGTGACG GCTTCTATGA CGACCAAGAC 
TATGAGTTCA ATCACCACGA GCCTCCATCC TACGAGCAAA GCCCTGTGCA GGGCAACCCT 
CACCCCCATT CCCCTAGGAC TTCCCGCCAC AACACCCCTC CTCAGGGCCA AGCGCACCCA 
CGGCGCATGC CAAATGGCTA CCGTTCGTCT TCGCCCTCTC CACACCACCA CGCGCCCGCT 
CACCCTGGAA CTCATAAACC TCCTCATCCC AGAGGGCCCA GAAAGGGCCT CCATGAACCC 
TACAGTGAGA CTGACGAGGA TGACTGGTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_021474381.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330056.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X4, mRNA.

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CloneID	ODa141786
Clone ID Related Accession (Same CDS sequence)	XM_021474381.1
Accession Version	XM_021474381.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA ACAACTTCAT AAATCTGAGT 
TTTCTAAGGC TCTTCAGAGC TGCTCGTCTC ATTAAGTTGC TCAGGCAGGG TGAAACCATA 
CGCATCCTGC TCTGGACTTT TGTCCAGTCT TTCAAGGCTC TGCCGTATGT GTGCCTACTC 
ATCGCCATGC TGTTCTTCAT CTACGCCATC ATTGGCATGC AGCTGTTTGG GAACATTAAG 
ATCGAGGAGA ACAGTGACAG TGCCATTACC CAGCACAACA ACTTCAGGAC TTTTTTCCAG 
GCCCTAATGC TGCTCTTTAG GAGTGCTACA GGTGAGGCAT GGCATGACAT CATGCTGTCA 
TGTCTGGGAA AGAAGCCGTG TGATATTCTC TCTGATAACC CGAAGCCAGA ATGCGGGAGC 
GAGTTTGCCT ACCTCTACTT TGTCTCCTTC ATCTTCCTCT GTTCATTTCT GATGCTGAAT 
CTGTTCGTGG CTGTGATCAT GGATAATTTT GAATACCTGA CGCGTGACTC CTCTATCCTG 
GGACCCCATC ACCTGGACGA GTATGTGAGG ATCTGGGCAG AATATGATCC TGCTGCTTGC 
GGGCGCATTC ATTATAAGGA TATGTACAGT TTATTGCGAG TTATCGACCC CCCTCTGGGC 
TTAGGCAAGA AATGTCCTCA TAGGGTGGCC TGCAAGAGAC TGTTGCGGAT GGATCTGCCG 
GTTGCCGATG ACAACACAGT GCACTTTAAT TCTACCCTTA TGGCCTTAAT CCGAACCGCT 
CTGGACATCA AGATCGCCAA GGGTGGGGAA GGAGGTGTAG ATAAGCACCA GATGGATGCT 
GAATTAAGGA AGGAGATGAT GGCGATATGG CCTAATCTCT CTCAGAAGAA CCTGGACCTG 
CTGGTGACTC CTCACAAGTC AGCGACAGAC CTGACAGTAG GAAAGATCTA CGCTGCCATG 
ATGATCATGG AGTATTATCG GCAAAGTAAA GCCAAACGGA CACAAGCTCT CCATGATGAG 
CAGAATCGAA CGCCATTAAT GTTTCAGCGT CTGGAGCCGC CATCTCCTAG CCAGGATGTG 
GGACCAGGAC TCACTGGCCT TCCTGACACA CAAATGCACC CTGCAAATCA TCTGCCTGTT 
GATGAAAGGA TCCCAGAGAG CCAATCATGG GTGACAGCGC GAGCACAGGA GATTTCTCAA 
AAAGCAGGCA ACTGGAGTCC AGAGGGCCAA CATCCAGATG ACACCACAGA CAACAGAAGG 
CAGTCTCAGA CAGTAGAGAT GAGAGAGATG GGGCGGGACG GGTACTCGGA CACAGAGCAA 
TATCTGCCGA TGGAAGGCCA TGGCAGGGCT GCTTCCATGC CTCGACTCCC AGCAGACAAC 
CAACAGCCAC GGAGGAAAGG GCGTCACCGG GGAGAAAATC TCACTCAGCC TATCACAGAT 
AACAGTCCTA TGAAGCGCTC GGCTTCATCT TTGGGACACG GGCGTGCAGG ACGCTCCATG 
CGAGGTGAGG ATTATGCCAT GGAGAGGGTT ATCCCAGAAG AGGGTCACAG GCATGGGCAT 
CGCCACCGAG ACCGTAGTCA CCGGGCGTCA GAACGCTCCC TGAGCCGATA CACTGATGCT 
GACACAGGTC TTGGAACTGA CCTCAGCACA ACCACACAAT CAGGAGACCT GCCATCCAAG 
GAGAGAGACC GGGGCCGAGC CAAAGACCGC AAGCACCATC ATCATCATCA TCACCACCAC 
GGCTCTTTAG ATAAGGAGCA CTACGGTCAT GAGAGAGACA GAGGAGAGTA CGGCCACAGA 
CAGTCCAGAG AGAGAGACCG CCGCTGGTCC CGCTCACCCA GTGAGGGCAG AGAGTGTCTG 
ACTCACAGAC AGGGCAGTAG TTCAGTAAGC GGCAGTCCAG TCCCGTCCAC CTCCGGGACC 
AGCACCCCCC GCCGTGGCCG CCGTCAGCTC CCCCAAACCC CTGCTACACC CCGGCCACAT 
GTCACCTACT CACCTGTGGT CCGTAAGCCC ATCAGCAGTA CCCCCCCTCC TGGCCAGCAG 
AGCCGACTCC CTACCCCGAC CTCCCGGCGC TTCAGTCCGA CTGGCCCCGA GCCCCCGCTT 
CCCCCTCATC ACCACCCGTC ACCACCCCAC CACGGCTCTC CCCGCTCCGG CCGACATGCA 
CACTGGGGCC CCGAGCCTGC AGAGAGCGTG GAGGGTGACG GCTTCTATGA CGACCAAGAC 
TATGAGTTCA ATCACCACGA GCCTCCATCC TACGAGCAAA GCCCTGTGCA GGGCAACCCT 
CACCCCCATT CCCCTAGGAC TTCCCGCCAC AACACCCCTC CTCAGGGCCA AGCGCACCCA 
CGGCGCATGC CAAATGGCTA CCGTTCGTCT TCGCCCTCTC CACACCACCA CGCGCCCGCT 
CACCCTGGAA CTCATAAACC TCCTCATCCC AGAGGGCCCA GAAAGGGCCT CCATGAACCC 
TACAGTGAGA CTGACGAGGA TGACTGGTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330056.1
CDS	1507..8619
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGGSGGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGEGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQNRTPLMFQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQTVEMREMGRDGYSDTEQYLPMEGHGRAASMPRLPADNQQPRRKGRHRGENLTQPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_021474381.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474381.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA ACAACTTCAT AAATCTGAGT 
TTTCTAAGGC TCTTCAGAGC TGCTCGTCTC ATTAAGTTGC TCAGGCAGGG TGAAACCATA 
CGCATCCTGC TCTGGACTTT TGTCCAGTCT TTCAAGGCTC TGCCGTATGT GTGCCTACTC 
ATCGCCATGC TGTTCTTCAT CTACGCCATC ATTGGCATGC AGCTGTTTGG GAACATTAAG 
ATCGAGGAGA ACAGTGACAG TGCCATTACC CAGCACAACA ACTTCAGGAC TTTTTTCCAG 
GCCCTAATGC TGCTCTTTAG GAGTGCTACA GGTGAGGCAT GGCATGACAT CATGCTGTCA 
TGTCTGGGAA AGAAGCCGTG TGATATTCTC TCTGATAACC CGAAGCCAGA ATGCGGGAGC 
GAGTTTGCCT ACCTCTACTT TGTCTCCTTC ATCTTCCTCT GTTCATTTCT GATGCTGAAT 
CTGTTCGTGG CTGTGATCAT GGATAATTTT GAATACCTGA CGCGTGACTC CTCTATCCTG 
GGACCCCATC ACCTGGACGA GTATGTGAGG ATCTGGGCAG AATATGATCC TGCTGCTTGC 
GGGCGCATTC ATTATAAGGA TATGTACAGT TTATTGCGAG TTATCGACCC CCCTCTGGGC 
TTAGGCAAGA AATGTCCTCA TAGGGTGGCC TGCAAGAGAC TGTTGCGGAT GGATCTGCCG 
GTTGCCGATG ACAACACAGT GCACTTTAAT TCTACCCTTA TGGCCTTAAT CCGAACCGCT 
CTGGACATCA AGATCGCCAA GGGTGGGGAA GGAGGTGTAG ATAAGCACCA GATGGATGCT 
GAATTAAGGA AGGAGATGAT GGCGATATGG CCTAATCTCT CTCAGAAGAA CCTGGACCTG 
CTGGTGACTC CTCACAAGTC AGCGACAGAC CTGACAGTAG GAAAGATCTA CGCTGCCATG 
ATGATCATGG AGTATTATCG GCAAAGTAAA GCCAAACGGA CACAAGCTCT CCATGATGAG 
CAGAATCGAA CGCCATTAAT GTTTCAGCGT CTGGAGCCGC CATCTCCTAG CCAGGATGTG 
GGACCAGGAC TCACTGGCCT TCCTGACACA CAAATGCACC CTGCAAATCA TCTGCCTGTT 
GATGAAAGGA TCCCAGAGAG CCAATCATGG GTGACAGCGC GAGCACAGGA GATTTCTCAA 
AAAGCAGGCA ACTGGAGTCC AGAGGGCCAA CATCCAGATG ACACCACAGA CAACAGAAGG 
CAGTCTCAGA CAGTAGAGAT GAGAGAGATG GGGCGGGACG GGTACTCGGA CACAGAGCAA 
TATCTGCCGA TGGAAGGCCA TGGCAGGGCT GCTTCCATGC CTCGACTCCC AGCAGACAAC 
CAACAGCCAC GGAGGAAAGG GCGTCACCGG GGAGAAAATC TCACTCAGCC TATCACAGAT 
AACAGTCCTA TGAAGCGCTC GGCTTCATCT TTGGGACACG GGCGTGCAGG ACGCTCCATG 
CGAGGTGAGG ATTATGCCAT GGAGAGGGTT ATCCCAGAAG AGGGTCACAG GCATGGGCAT 
CGCCACCGAG ACCGTAGTCA CCGGGCGTCA GAACGCTCCC TGAGCCGATA CACTGATGCT 
GACACAGGTC TTGGAACTGA CCTCAGCACA ACCACACAAT CAGGAGACCT GCCATCCAAG 
GAGAGAGACC GGGGCCGAGC CAAAGACCGC AAGCACCATC ATCATCATCA TCACCACCAC 
GGCTCTTTAG ATAAGGAGCA CTACGGTCAT GAGAGAGACA GAGGAGAGTA CGGCCACAGA 
CAGTCCAGAG AGAGAGACCG CCGCTGGTCC CGCTCACCCA GTGAGGGCAG AGAGTGTCTG 
ACTCACAGAC AGGGCAGTAG TTCAGTAAGC GGCAGTCCAG TCCCGTCCAC CTCCGGGACC 
AGCACCCCCC GCCGTGGCCG CCGTCAGCTC CCCCAAACCC CTGCTACACC CCGGCCACAT 
GTCACCTACT CACCTGTGGT CCGTAAGCCC ATCAGCAGTA CCCCCCCTCC TGGCCAGCAG 
AGCCGACTCC CTACCCCGAC CTCCCGGCGC TTCAGTCCGA CTGGCCCCGA GCCCCCGCTT 
CCCCCTCATC ACCACCCGTC ACCACCCCAC CACGGCTCTC CCCGCTCCGG CCGACATGCA 
CACTGGGGCC CCGAGCCTGC AGAGAGCGTG GAGGGTGACG GCTTCTATGA CGACCAAGAC 
TATGAGTTCA ATCACCACGA GCCTCCATCC TACGAGCAAA GCCCTGTGCA GGGCAACCCT 
CACCCCCATT CCCCTAGGAC TTCCCGCCAC AACACCCCTC CTCAGGGCCA AGCGCACCCA 
CGGCGCATGC CAAATGGCTA CCGTTCGTCT TCGCCCTCTC CACACCACCA CGCGCCCGCT 
CACCCTGGAA CTCATAAACC TCCTCATCCC AGAGGGCCCA GAAAGGGCCT CCATGAACCC 
TACAGTGAGA CTGACGAGGA TGACTGGTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_021474386.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330061.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X5, mRNA.

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CloneID	ODa141787
Clone ID Related Accession (Same CDS sequence)	XM_021474386.1
Accession Version	XM_021474386.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG 
GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT 
GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC 
CTGCTGGTGA CTCCTCACAA GTCGACAGAC CTGACAGTAG GAAAGATCTA CGCTGCCATG 
ATGATCATGG AGTATTATCG GCAAAGTAAA GCCAAACGGA CACAAGCTCT CCATGATGAG 
CAGAATCGAA CGCCATTAAT GTTTCAGCGT CTGGAGCCGC CATCTCCTAG CCAGGATGTG 
GGACCAGGAC TCACTGGCCT TCCTGACACA CAAATGCACC CTGCAAATCA TCTGCCTGTT 
GATGAAAGGA TCCCAGAGAG CCAATCATGG GTGACAGCGC GAGCACAGGA GATTTCTCAA 
AAAGCAGGCA ACTGGAGTCC AGAGGGCCAA CATCCAGATG ACACCACAGA CAACAGAAGG 
CAGTCTCAGA CAGTAGAGAT GAGAGAGATG GGGCGGGACG GGTACTCGGA CACAGAGCAA 
TATCTGCCGA TGGAAGGCCA TGGCAGGGCT GCTTCCATGC CTCGACTCCC AGCAGACAAC 
CAACAGCCAC GGAGGAAAGG GCGTCACCGG GGAGAAAATC TCACTCAGCC TATCACAGAT 
AACAGTCCTA TGAAGCGCTC GGCTTCATCT TTGGGACACG GGCGTGCAGG ACGCTCCATG 
CGAGGTGAGG ATTATGCCAT GGAGAGGGTT ATCCCAGAAG AGGGTCACAG GCATGGGCAT 
CGCCACCGAG ACCGTAGTCA CCGGGCGTCA GAACGCTCCC TGAGCCGATA CACTGATGCT 
GACACAGGTC TTGGAACTGA CCTCAGCACA ACCACACAAT CAGGAGACCT GCCATCCAAG 
GAGAGAGACC GGGGCCGAGC CAAAGACCGC AAGCACCATC ATCATCATCA TCACCACCAC 
GGCTCTTTAG ATAAGGAGCA CTACGGTCAT GAGAGAGACA GAGGAGAGTA CGGCCACAGA 
CAGTCCAGAG AGAGAGACCG CCGCTGGTCC CGCTCACCCA GTGAGGGCAG AGAGTGTCTG 
ACTCACAGAC AGGGCAGTAG TTCAGTAAGC GGCAGTCCAG TCCCGTCCAC CTCCGGGACC 
AGCACCCCCC GCCGTGGCCG CCGTCAGCTC CCCCAAACCC CTGCTACACC CCGGCCACAT 
GTCACCTACT CACCTGTGGT CCGTAAGCCC ATCAGCAGTA CCCCCCCTCC TGGCCAGCAG 
AGCCGACTCC CTACCCCGAC CTCCCGGCGC TTCAGTCCGA CTGGCCCCGA GCCCCCGCTT 
CCCCCTCATC ACCACCCGTC ACCACCCCAC CACGGCTCTC CCCGCTCCGG CCGACATGCA 
CACTGGGGCC CCGAGCCTGC AGAGAGCGTG GAGGGTGACG GCTTCTATGA CGACCAAGAC 
TATGAGTTCA ATCACCACGA GCCTCCATCC TACGAGCAAA GCCCTGTGCA GGGCAACCCT 
CACCCCCATT CCCCTAGGAC TTCCCGCCAC AACACCCCTC CTCAGGGCCA AGCGCACCCA 
CGGCGCATGC CAAATGGCTA CCGTTCGTCT TCGCCCTCTC CACACCACCA CGCGCCCGCT 
CACCCTGGAA CTCATAAACC TCCTCATCCC AGAGGGCCCA GAAAGGGCCT CCATGAACCC 
TACAGTGAGA CTGACGAGGA TGACTGGTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330061.1
CDS	1508..8620
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGGSGGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGMNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGEGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQNRTPLMFQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQTVEMREMGRDGYSDTEQYLPMEGHGRAASMPRLPADNQQPRRKGRHRGENLTQPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_021474386.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474386.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG 
GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT 
GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC 
CTGCTGGTGA CTCCTCACAA GTCGACAGAC CTGACAGTAG GAAAGATCTA CGCTGCCATG 
ATGATCATGG AGTATTATCG GCAAAGTAAA GCCAAACGGA CACAAGCTCT CCATGATGAG 
CAGAATCGAA CGCCATTAAT GTTTCAGCGT CTGGAGCCGC CATCTCCTAG CCAGGATGTG 
GGACCAGGAC TCACTGGCCT TCCTGACACA CAAATGCACC CTGCAAATCA TCTGCCTGTT 
GATGAAAGGA TCCCAGAGAG CCAATCATGG GTGACAGCGC GAGCACAGGA GATTTCTCAA 
AAAGCAGGCA ACTGGAGTCC AGAGGGCCAA CATCCAGATG ACACCACAGA CAACAGAAGG 
CAGTCTCAGA CAGTAGAGAT GAGAGAGATG GGGCGGGACG GGTACTCGGA CACAGAGCAA 
TATCTGCCGA TGGAAGGCCA TGGCAGGGCT GCTTCCATGC CTCGACTCCC AGCAGACAAC 
CAACAGCCAC GGAGGAAAGG GCGTCACCGG GGAGAAAATC TCACTCAGCC TATCACAGAT 
AACAGTCCTA TGAAGCGCTC GGCTTCATCT TTGGGACACG GGCGTGCAGG ACGCTCCATG 
CGAGGTGAGG ATTATGCCAT GGAGAGGGTT ATCCCAGAAG AGGGTCACAG GCATGGGCAT 
CGCCACCGAG ACCGTAGTCA CCGGGCGTCA GAACGCTCCC TGAGCCGATA CACTGATGCT 
GACACAGGTC TTGGAACTGA CCTCAGCACA ACCACACAAT CAGGAGACCT GCCATCCAAG 
GAGAGAGACC GGGGCCGAGC CAAAGACCGC AAGCACCATC ATCATCATCA TCACCACCAC 
GGCTCTTTAG ATAAGGAGCA CTACGGTCAT GAGAGAGACA GAGGAGAGTA CGGCCACAGA 
CAGTCCAGAG AGAGAGACCG CCGCTGGTCC CGCTCACCCA GTGAGGGCAG AGAGTGTCTG 
ACTCACAGAC AGGGCAGTAG TTCAGTAAGC GGCAGTCCAG TCCCGTCCAC CTCCGGGACC 
AGCACCCCCC GCCGTGGCCG CCGTCAGCTC CCCCAAACCC CTGCTACACC CCGGCCACAT 
GTCACCTACT CACCTGTGGT CCGTAAGCCC ATCAGCAGTA CCCCCCCTCC TGGCCAGCAG 
AGCCGACTCC CTACCCCGAC CTCCCGGCGC TTCAGTCCGA CTGGCCCCGA GCCCCCGCTT 
CCCCCTCATC ACCACCCGTC ACCACCCCAC CACGGCTCTC CCCGCTCCGG CCGACATGCA 
CACTGGGGCC CCGAGCCTGC AGAGAGCGTG GAGGGTGACG GCTTCTATGA CGACCAAGAC 
TATGAGTTCA ATCACCACGA GCCTCCATCC TACGAGCAAA GCCCTGTGCA GGGCAACCCT 
CACCCCCATT CCCCTAGGAC TTCCCGCCAC AACACCCCTC CTCAGGGCCA AGCGCACCCA 
CGGCGCATGC CAAATGGCTA CCGTTCGTCT TCGCCCTCTC CACACCACCA CGCGCCCGCT 
CACCCTGGAA CTCATAAACC TCCTCATCCC AGAGGGCCCA GAAAGGGCCT CCATGAACCC 
TACAGTGAGA CTGACGAGGA TGACTGGTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_021474390.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330065.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X6, mRNA.

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CloneID	ODa141788
Clone ID Related Accession (Same CDS sequence)	XM_021474390.1
Accession Version	XM_021474390.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG 
GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGAGGT GTAGATAAGC ACCAGATGGA TGCTGAATTA 
AGGAAGGAGA TGATGGCGAT ATGGCCTAAT CTCTCTCAGA AGAACCTGGA CCTGCTGGTG 
ACTCCTCACA AGTCAGCGAC AGACCTGACA GTAGGAAAGA TCTACGCTGC CATGATGATC 
ATGGAGTATT ATCGGCAAAG TAAAGCCAAA CGGACACAAG CTCTCCATGA TGAGCAGAAT 
CGAACGCCAT TAATGTTTCA GCGTCTGGAG CCGCCATCTC CTAGCCAGGA TGTGGGACCA 
GGACTCACTG GCCTTCCTGA CACACAAATG CACCCTGCAA ATCATCTGCC TGTTGATGAA 
AGGATCCCAG AGAGCCAATC ATGGGTGACA GCGCGAGCAC AGGAGATTTC TCAAAAAGCA 
GGCAACTGGA GTCCAGAGGG CCAACATCCA GATGACACCA CAGACAACAG AAGGCAGTCT 
CAGACAGTAG AGATGAGAGA GATGGGGCGG GACGGGTACT CGGACACAGA GCAATATCTG 
CCGATGGAAG GCCATGGCAG GGCTGCTTCC ATGCCTCGAC TCCCAGCAGA CAACCAACAG 
CCACGGAGGA AAGGGCGTCA CCGGGGAGAA AATCTCACTC AGCCTATCAC AGATAACAGT 
CCTATGAAGC GCTCGGCTTC ATCTTTGGGA CACGGGCGTG CAGGACGCTC CATGCGAGGT 
GAGGATTATG CCATGGAGAG GGTTATCCCA GAAGAGGGTC ACAGGCATGG GCATCGCCAC 
CGAGACCGTA GTCACCGGGC GTCAGAACGC TCCCTGAGCC GATACACTGA TGCTGACACA 
GGTCTTGGAA CTGACCTCAG CACAACCACA CAATCAGGAG ACCTGCCATC CAAGGAGAGA 
GACCGGGGCC GAGCCAAAGA CCGCAAGCAC CATCATCATC ATCATCACCA CCACGGCTCT 
TTAGATAAGG AGCACTACGG TCATGAGAGA GACAGAGGAG AGTACGGCCA CAGACAGTCC 
AGAGAGAGAG ACCGCCGCTG GTCCCGCTCA CCCAGTGAGG GCAGAGAGTG TCTGACTCAC 
AGACAGGGCA GTAGTTCAGT AAGCGGCAGT CCAGTCCCGT CCACCTCCGG GACCAGCACC 
CCCCGCCGTG GCCGCCGTCA GCTCCCCCAA ACCCCTGCTA CACCCCGGCC ACATGTCACC 
TACTCACCTG TGGTCCGTAA GCCCATCAGC AGTACCCCCC CTCCTGGCCA GCAGAGCCGA 
CTCCCTACCC CGACCTCCCG GCGCTTCAGT CCGACTGGCC CCGAGCCCCC GCTTCCCCCT 
CATCACCACC CGTCACCACC CCACCACGGC TCTCCCCGCT CCGGCCGACA TGCACACTGG 
GGCCCCGAGC CTGCAGAGAG CGTGGAGGGT GACGGCTTCT ATGACGACCA AGACTATGAG 
TTCAATCACC ACGAGCCTCC ATCCTACGAG CAAAGCCCTG TGCAGGGCAA CCCTCACCCC 
CATTCCCCTA GGACTTCCCG CCACAACACC CCTCCTCAGG GCCAAGCGCA CCCACGGCGC 
ATGCCAAATG GCTACCGTTC GTCTTCGCCC TCTCCACACC ACCACGCGCC CGCTCACCCT 
GGAACTCATA AACCTCCTCA TCCCAGAGGG CCCAGAAAGG GCCTCCATGA ACCCTACAGT 
GAGACTGACG AGGATGACTG GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330065.1
CDS	1509..8615
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGGSGGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGMNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQNRTPLMFQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQTVEMREMGRDGYSDTEQYLPMEGHGRAASMPRLPADNQQPRRKGRHRGENLTQPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_021474390.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474390.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG 
GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGAGGT GTAGATAAGC ACCAGATGGA TGCTGAATTA 
AGGAAGGAGA TGATGGCGAT ATGGCCTAAT CTCTCTCAGA AGAACCTGGA CCTGCTGGTG 
ACTCCTCACA AGTCAGCGAC AGACCTGACA GTAGGAAAGA TCTACGCTGC CATGATGATC 
ATGGAGTATT ATCGGCAAAG TAAAGCCAAA CGGACACAAG CTCTCCATGA TGAGCAGAAT 
CGAACGCCAT TAATGTTTCA GCGTCTGGAG CCGCCATCTC CTAGCCAGGA TGTGGGACCA 
GGACTCACTG GCCTTCCTGA CACACAAATG CACCCTGCAA ATCATCTGCC TGTTGATGAA 
AGGATCCCAG AGAGCCAATC ATGGGTGACA GCGCGAGCAC AGGAGATTTC TCAAAAAGCA 
GGCAACTGGA GTCCAGAGGG CCAACATCCA GATGACACCA CAGACAACAG AAGGCAGTCT 
CAGACAGTAG AGATGAGAGA GATGGGGCGG GACGGGTACT CGGACACAGA GCAATATCTG 
CCGATGGAAG GCCATGGCAG GGCTGCTTCC ATGCCTCGAC TCCCAGCAGA CAACCAACAG 
CCACGGAGGA AAGGGCGTCA CCGGGGAGAA AATCTCACTC AGCCTATCAC AGATAACAGT 
CCTATGAAGC GCTCGGCTTC ATCTTTGGGA CACGGGCGTG CAGGACGCTC CATGCGAGGT 
GAGGATTATG CCATGGAGAG GGTTATCCCA GAAGAGGGTC ACAGGCATGG GCATCGCCAC 
CGAGACCGTA GTCACCGGGC GTCAGAACGC TCCCTGAGCC GATACACTGA TGCTGACACA 
GGTCTTGGAA CTGACCTCAG CACAACCACA CAATCAGGAG ACCTGCCATC CAAGGAGAGA 
GACCGGGGCC GAGCCAAAGA CCGCAAGCAC CATCATCATC ATCATCACCA CCACGGCTCT 
TTAGATAAGG AGCACTACGG TCATGAGAGA GACAGAGGAG AGTACGGCCA CAGACAGTCC 
AGAGAGAGAG ACCGCCGCTG GTCCCGCTCA CCCAGTGAGG GCAGAGAGTG TCTGACTCAC 
AGACAGGGCA GTAGTTCAGT AAGCGGCAGT CCAGTCCCGT CCACCTCCGG GACCAGCACC 
CCCCGCCGTG GCCGCCGTCA GCTCCCCCAA ACCCCTGCTA CACCCCGGCC ACATGTCACC 
TACTCACCTG TGGTCCGTAA GCCCATCAGC AGTACCCCCC CTCCTGGCCA GCAGAGCCGA 
CTCCCTACCC CGACCTCCCG GCGCTTCAGT CCGACTGGCC CCGAGCCCCC GCTTCCCCCT 
CATCACCACC CGTCACCACC CCACCACGGC TCTCCCCGCT CCGGCCGACA TGCACACTGG 
GGCCCCGAGC CTGCAGAGAG CGTGGAGGGT GACGGCTTCT ATGACGACCA AGACTATGAG 
TTCAATCACC ACGAGCCTCC ATCCTACGAG CAAAGCCCTG TGCAGGGCAA CCCTCACCCC 
CATTCCCCTA GGACTTCCCG CCACAACACC CCTCCTCAGG GCCAAGCGCA CCCACGGCGC 
ATGCCAAATG GCTACCGTTC GTCTTCGCCC TCTCCACACC ACCACGCGCC CGCTCACCCT 
GGAACTCATA AACCTCCTCA TCCCAGAGGG CCCAGAAAGG GCCTCCATGA ACCCTACAGT 
GAGACTGACG AGGATGACTG GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_021474393.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330068.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X7, mRNA.

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CloneID	ODa141789
Clone ID Related Accession (Same CDS sequence)	XM_021474393.1
Accession Version	XM_021474393.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGTGGCAGAA TCAGTTACAG GGATATGTAT GAGATGCTCA GGCACATGTC TCCTCCGTTA 
GGCCTGGGGA AGAAGTGCCC TGCACGGGTT GCCTACAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGAGGT GTAGATAAGC ACCAGATGGA TGCTGAATTA 
AGGAAGGAGA TGATGGCGAT ATGGCCTAAT CTCTCTCAGA AGAACCTGGA CCTGCTGGTG 
ACTCCTCACA AGTCAGCGAC AGACCTGACA GTAGGAAAGA TCTACGCTGC CATGATGATC 
ATGGAGTATT ATCGGCAAAG TAAAGCCAAA CGGACACAAG CTCTCCATGA TGAGCAGAAT 
CGAACGCCAT TAATGTTTCA GCGTCTGGAG CCGCCATCTC CTAGCCAGGA TGTGGGACCA 
GGACTCACTG GCCTTCCTGA CACACAAATG CACCCTGCAA ATCATCTGCC TGTTGATGAA 
AGGATCCCAG AGAGCCAATC ATGGGTGACA GCGCGAGCAC AGGAGATTTC TCAAAAAGCA 
GGCAACTGGA GTCCAGAGGG CCAACATCCA GATGACACCA CAGACAACAG AAGGCAGTCT 
CAGACAGTAG AGATGAGAGA GATGGGGCGG GACGGGTACT CGGACACAGA GCAATATCTG 
CCGATGGAAG GCCATGGCAG GGCTGCTTCC ATGCCTCGAC TCCCAGCAGA CAACCAACAG 
CCACGGAGGA AAGGGCGTCA CCGGGGAGAA AATCTCACTC AGCCTATCAC AGATAACAGT 
CCTATGAAGC GCTCGGCTTC ATCTTTGGGA CACGGGCGTG CAGGACGCTC CATGCGAGGT 
GAGGATTATG CCATGGAGAG GGTTATCCCA GAAGAGGGTC ACAGGCATGG GCATCGCCAC 
CGAGACCGTA GTCACCGGGC GTCAGAACGC TCCCTGAGCC GATACACTGA TGCTGACACA 
GGTCTTGGAA CTGACCTCAG CACAACCACA CAATCAGGAG ACCTGCCATC CAAGGAGAGA 
GACCGGGGCC GAGCCAAAGA CCGCAAGCAC CATCATCATC ATCATCACCA CCACGGCTCT 
TTAGATAAGG AGCACTACGG TCATGAGAGA GACAGAGGAG AGTACGGCCA CAGACAGTCC 
AGAGAGAGAG ACCGCCGCTG GTCCCGCTCA CCCAGTGAGG GCAGAGAGTG TCTGACTCAC 
AGACAGGGCA GTAGTTCAGT AAGCGGCAGT CCAGTCCCGT CCACCTCCGG GACCAGCACC 
CCCCGCCGTG GCCGCCGTCA GCTCCCCCAA ACCCCTGCTA CACCCCGGCC ACATGTCACC 
TACTCACCTG TGGTCCGTAA GCCCATCAGC AGTACCCCCC CTCCTGGCCA GCAGAGCCGA 
CTCCCTACCC CGACCTCCCG GCGCTTCAGT CCGACTGGCC CCGAGCCCCC GCTTCCCCCT 
CATCACCACC CGTCACCACC CCACCACGGC TCTCCCCGCT CCGGCCGACA TGCACACTGG 
GGCCCCGAGC CTGCAGAGAG CGTGGAGGGT GACGGCTTCT ATGACGACCA AGACTATGAG 
TTCAATCACC ACGAGCCTCC ATCCTACGAG CAAAGCCCTG TGCAGGGCAA CCCTCACCCC 
CATTCCCCTA GGACTTCCCG CCACAACACC CCTCCTCAGG GCCAAGCGCA CCCACGGCGC 
ATGCCAAATG GCTACCGTTC GTCTTCGCCC TCTCCACACC ACCACGCGCC CGCTCACCCT 
GGAACTCATA AACCTCCTCA TCCCAGAGGG CCCAGAAAGG GCCTCCATGA ACCCTACAGT 
GAGACTGACG AGGATGACTG GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330068.1
CDS	1509..8615
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGGSGGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGMNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRISYRDMYEMLRHMSPPLGLGKKCPARVAYKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQNRTPLMFQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQTVEMREMGRDGYSDTEQYLPMEGHGRAASMPRLPADNQQPRRKGRHRGENLTQPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_021474393.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474393.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGTGGCAGAA TCAGTTACAG GGATATGTAT GAGATGCTCA GGCACATGTC TCCTCCGTTA 
GGCCTGGGGA AGAAGTGCCC TGCACGGGTT GCCTACAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGAGGT GTAGATAAGC ACCAGATGGA TGCTGAATTA 
AGGAAGGAGA TGATGGCGAT ATGGCCTAAT CTCTCTCAGA AGAACCTGGA CCTGCTGGTG 
ACTCCTCACA AGTCAGCGAC AGACCTGACA GTAGGAAAGA TCTACGCTGC CATGATGATC 
ATGGAGTATT ATCGGCAAAG TAAAGCCAAA CGGACACAAG CTCTCCATGA TGAGCAGAAT 
CGAACGCCAT TAATGTTTCA GCGTCTGGAG CCGCCATCTC CTAGCCAGGA TGTGGGACCA 
GGACTCACTG GCCTTCCTGA CACACAAATG CACCCTGCAA ATCATCTGCC TGTTGATGAA 
AGGATCCCAG AGAGCCAATC ATGGGTGACA GCGCGAGCAC AGGAGATTTC TCAAAAAGCA 
GGCAACTGGA GTCCAGAGGG CCAACATCCA GATGACACCA CAGACAACAG AAGGCAGTCT 
CAGACAGTAG AGATGAGAGA GATGGGGCGG GACGGGTACT CGGACACAGA GCAATATCTG 
CCGATGGAAG GCCATGGCAG GGCTGCTTCC ATGCCTCGAC TCCCAGCAGA CAACCAACAG 
CCACGGAGGA AAGGGCGTCA CCGGGGAGAA AATCTCACTC AGCCTATCAC AGATAACAGT 
CCTATGAAGC GCTCGGCTTC ATCTTTGGGA CACGGGCGTG CAGGACGCTC CATGCGAGGT 
GAGGATTATG CCATGGAGAG GGTTATCCCA GAAGAGGGTC ACAGGCATGG GCATCGCCAC 
CGAGACCGTA GTCACCGGGC GTCAGAACGC TCCCTGAGCC GATACACTGA TGCTGACACA 
GGTCTTGGAA CTGACCTCAG CACAACCACA CAATCAGGAG ACCTGCCATC CAAGGAGAGA 
GACCGGGGCC GAGCCAAAGA CCGCAAGCAC CATCATCATC ATCATCACCA CCACGGCTCT 
TTAGATAAGG AGCACTACGG TCATGAGAGA GACAGAGGAG AGTACGGCCA CAGACAGTCC 
AGAGAGAGAG ACCGCCGCTG GTCCCGCTCA CCCAGTGAGG GCAGAGAGTG TCTGACTCAC 
AGACAGGGCA GTAGTTCAGT AAGCGGCAGT CCAGTCCCGT CCACCTCCGG GACCAGCACC 
CCCCGCCGTG GCCGCCGTCA GCTCCCCCAA ACCCCTGCTA CACCCCGGCC ACATGTCACC 
TACTCACCTG TGGTCCGTAA GCCCATCAGC AGTACCCCCC CTCCTGGCCA GCAGAGCCGA 
CTCCCTACCC CGACCTCCCG GCGCTTCAGT CCGACTGGCC CCGAGCCCCC GCTTCCCCCT 
CATCACCACC CGTCACCACC CCACCACGGC TCTCCCCGCT CCGGCCGACA TGCACACTGG 
GGCCCCGAGC CTGCAGAGAG CGTGGAGGGT GACGGCTTCT ATGACGACCA AGACTATGAG 
TTCAATCACC ACGAGCCTCC ATCCTACGAG CAAAGCCCTG TGCAGGGCAA CCCTCACCCC 
CATTCCCCTA GGACTTCCCG CCACAACACC CCTCCTCAGG GCCAAGCGCA CCCACGGCGC 
ATGCCAAATG GCTACCGTTC GTCTTCGCCC TCTCCACACC ACCACGCGCC CGCTCACCCT 
GGAACTCATA AACCTCCTCA TCCCAGAGGG CCCAGAAAGG GCCTCCATGA ACCCTACAGT 
GAGACTGACG AGGATGACTG GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_021474394.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330069.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X8, mRNA.

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CloneID	ODa141790
Clone ID Related Accession (Same CDS sequence)	XM_021474394.1
Accession Version	XM_021474394.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACAGGAAGC AGCAAAGGGA AAGACATCAG TACTATCAAG 
TCTCTACGTG TGCTTCGAGT ACTGCGGCCG CTGAAAACCA TCAAACGTCT GCCAAAACTG 
AAGGCTGTCT TTGACTGTGT GGTGAACTCT CTGAAGAATG TGTTGAACAT CCTCATCGTC 
TACATGCTCT TCATGTTCAT CTTCGCTGTG GTGGCTGTGC AGCTCTTTAA GGGACGTTTC 
TTTTACTGCA CTGATGAATC CAAAGAGTTT GAGCGCGACT GCAGGGGCGA GTATCTGGTC 
TATGAAAGAG ATAACGAAGT GAGGTCACAG AAGCGGGAAT GGAAGAAATA CGATTTCCAC 
TACGACAACG TGCTCTGGGC CCTTCTCACC CTCTTCACCG TTTCTACGGG AGAGGGGTGG 
CCGCAAGTGC TAAAACATTC AGTAGATGCG ACCTACGAGA ATCAGGGCCC GAGCCCAGGT 
TACCGGATGG AAATGTCCAT CTTTTACGTG GTGTACTTCG TGGTCTTCCC CTTCTTCTTC 
GTCAACATCT TCGTGGCTCT TATCATCATT ACTTTCCAGG AGCAAGGAGA CAAAATGATG 
GAGGACTATA GCCTGGAAAA GAATGAGAGA GCCTGCATAG ACTTTGCCAT CAACGCTAAG 
CCGCTCACAC GGCACATGCC CCAGAACAAG CAGACCTTTC AGTACCGCAT GTGGGAGTTT 
GTGGTGTCTC CTCCATTTGA ATACACCATC ATGGCCTTGA TCGCGCTCAA CACTATTGTC 
CTTATGATGA AGTATGATGG AGCATCGCTT ACTTATGAAG ATGTGCTAAA ATACCTGAAC 
ATAGTGTTCA CCTCTCTATT CTCCATGGAG TGCATATTGA AGATCATAGC CTTCGGAGCA 
TTGAACTACT TCAAAGATGC CTGGAACATC TTCGACTGTG TGACTGTACT TGGCAGCATC 
ACTGATATTT TAGTCACAGA GCTGGGGATG AACAACTTCA TAAATCTGAG TTTTCTAAGG 
CTCTTCAGAG CTGCTCGTCT CATTAAGTTG CTCAGGCAGG GTGAAACCAT ACGCATCCTG 
CTCTGGACTT TTGTCCAGTC TTTCAAGGCT CTGCCGTATG TGTGCCTACT CATCGCCATG 
CTGTTCTTCA TCTACGCCAT CATTGGCATG CAGCTGTTTG GGAACATTAA GATCGAGGAG 
AACAGTGACA GTGCCATTAC CCAGCACAAC AACTTCAGGA CTTTTTTCCA GGCCCTAATG 
CTGCTCTTTA GGAGTGCTAC AGGTGAGGCA TGGCATGACA TCATGCTGTC ATGTCTGGGA 
AAGAAGCCGT GTGATATTCT CTCTGATAAC CCGAAGCCAG AATGCGGGAG CGAGTTTGCC 
TACCTCTACT TTGTCTCCTT CATCTTCCTC TGTTCATTTC TGATGCTGAA TCTGTTCGTG 
GCTGTGATCA TGGATAATTT TGAATACCTG ACGCGTGACT CCTCTATCCT GGGACCCCAT 
CACCTGGACG AGTATGTGAG GATCTGGGCA GAATATGATC CTGCTGCTTG CGGGCGCATT 
CATTATAAGG ATATGTACAG TTTATTGCGA GTTATCGACC CCCCTCTGGG CTTAGGCAAG 
AAATGTCCTC ATAGGGTGGC CTGCAAGAGA CTGTTGCGGA TGGATCTGCC GGTTGCCGAT 
GACAACACAG TGCACTTTAA TTCTACCCTT ATGGCCTTAA TCCGAACCGC TCTGGACATC 
AAGATCGCCA AGGGTGGGGA AGGAGGTGTA GATAAGCACC AGATGGATGC TGAATTAAGG 
AAGGAGATGA TGGCGATATG GCCTAATCTC TCTCAGAAGA ACCTGGACCT GCTGGTGACT 
CCTCACAAGT CAGCGACAGA CCTGACAGTA GGAAAGATCT ACGCTGCCAT GATGATCATG 
GAGTATTATC GGCAAAGTAA AGCCAAACGG ACACAAGCTC TCCATGATGA GCAGAATCGA 
ACGCCATTAA TGTTTCAGCG TCTGGAGCCG CCATCTCCTA GCCAGGATGT GGGACCAGGA 
CTCACTGGCC TTCCTGACAC ACAAATGCAC CCTGCAAATC ATCTGCCTGT TGATGAAAGG 
ATCCCAGAGA GCCAATCATG GGTGACAGCG CGAGCACAGG AGATTTCTCA AAAAGCAGGC 
AACTGGAGTC CAGAGGGCCA ACATCCAGAT GACACCACAG ACAACAGAAG GCAGTCTCAG 
ACAGTAGAGA TGAGAGAGAT GGGGCGGGAC GGGTACTCGG ACACAGAGCA ATATCTGCCG 
ATGGAAGGCC ATGGCAGGGC TGCTTCCATG CCTCGACTCC CAGCAGACAA CCAACAGCCA 
CGGAGGAAAG GGCGTCACCG GGGAGAAAAT CTCACTCAGC CTATCACAGA TAACAGTCCT 
ATGAAGCGCT CGGCTTCATC TTTGGGACAC GGGCGTGCAG GACGCTCCAT GCGAGGTGAG 
GATTATGCCA TGGAGAGGGT TATCCCAGAA GAGGGTCACA GGCATGGGCA TCGCCACCGA 
GACCGTAGTC ACCGGGCGTC AGAACGCTCC CTGAGCCGAT ACACTGATGC TGACACAGGT 
CTTGGAACTG ACCTCAGCAC AACCACACAA TCAGGAGACC TGCCATCCAA GGAGAGAGAC 
CGGGGCCGAG CCAAAGACCG CAAGCACCAT CATCATCATC ATCACCACCA CGGCTCTTTA 
GATAAGGAGC ACTACGGTCA TGAGAGAGAC AGAGGAGAGT ACGGCCACAG ACAGTCCAGA 
GAGAGAGACC GCCGCTGGTC CCGCTCACCC AGTGAGGGCA GAGAGTGTCT GACTCACAGA 
CAGGGCAGTA GTTCAGTAAG CGGCAGTCCA GTCCCGTCCA CCTCCGGGAC CAGCACCCCC 
CGCCGTGGCC GCCGTCAGCT CCCCCAAACC CCTGCTACAC CCCGGCCACA TGTCACCTAC 
TCACCTGTGG TCCGTAAGCC CATCAGCAGT ACCCCCCCTC CTGGCCAGCA GAGCCGACTC 
CCTACCCCGA CCTCCCGGCG CTTCAGTCCG ACTGGCCCCG AGCCCCCGCT TCCCCCTCAT 
CACCACCCGT CACCACCCCA CCACGGCTCT CCCCGCTCCG GCCGACATGC ACACTGGGGC 
CCCGAGCCTG CAGAGAGCGT GGAGGGTGAC GGCTTCTATG ACGACCAAGA CTATGAGTTC 
AATCACCACG AGCCTCCATC CTACGAGCAA AGCCCTGTGC AGGGCAACCC TCACCCCCAT 
TCCCCTAGGA CTTCCCGCCA CAACACCCCT CCTCAGGGCC AAGCGCACCC ACGGCGCATG 
CCAAATGGCT ACCGTTCGTC TTCGCCCTCT CCACACCACC ACGCGCCCGC TCACCCTGGA 
ACTCATAAAC CTCCTCATCC CAGAGGGCCC AGAAAGGGCC TCCATGAACC CTACAGTGAG 
ACTGACGAGG ATGACTGGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330069.1
CDS	1507..8610
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGMNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGEGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQNRTPLMFQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQTVEMREMGRDGYSDTEQYLPMEGHGRAASMPRLPADNQQPRRKGRHRGENLTQPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_021474394.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474394.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACAGGAAGC AGCAAAGGGA AAGACATCAG TACTATCAAG 
TCTCTACGTG TGCTTCGAGT ACTGCGGCCG CTGAAAACCA TCAAACGTCT GCCAAAACTG 
AAGGCTGTCT TTGACTGTGT GGTGAACTCT CTGAAGAATG TGTTGAACAT CCTCATCGTC 
TACATGCTCT TCATGTTCAT CTTCGCTGTG GTGGCTGTGC AGCTCTTTAA GGGACGTTTC 
TTTTACTGCA CTGATGAATC CAAAGAGTTT GAGCGCGACT GCAGGGGCGA GTATCTGGTC 
TATGAAAGAG ATAACGAAGT GAGGTCACAG AAGCGGGAAT GGAAGAAATA CGATTTCCAC 
TACGACAACG TGCTCTGGGC CCTTCTCACC CTCTTCACCG TTTCTACGGG AGAGGGGTGG 
CCGCAAGTGC TAAAACATTC AGTAGATGCG ACCTACGAGA ATCAGGGCCC GAGCCCAGGT 
TACCGGATGG AAATGTCCAT CTTTTACGTG GTGTACTTCG TGGTCTTCCC CTTCTTCTTC 
GTCAACATCT TCGTGGCTCT TATCATCATT ACTTTCCAGG AGCAAGGAGA CAAAATGATG 
GAGGACTATA GCCTGGAAAA GAATGAGAGA GCCTGCATAG ACTTTGCCAT CAACGCTAAG 
CCGCTCACAC GGCACATGCC CCAGAACAAG CAGACCTTTC AGTACCGCAT GTGGGAGTTT 
GTGGTGTCTC CTCCATTTGA ATACACCATC ATGGCCTTGA TCGCGCTCAA CACTATTGTC 
CTTATGATGA AGTATGATGG AGCATCGCTT ACTTATGAAG ATGTGCTAAA ATACCTGAAC 
ATAGTGTTCA CCTCTCTATT CTCCATGGAG TGCATATTGA AGATCATAGC CTTCGGAGCA 
TTGAACTACT TCAAAGATGC CTGGAACATC TTCGACTGTG TGACTGTACT TGGCAGCATC 
ACTGATATTT TAGTCACAGA GCTGGGGATG AACAACTTCA TAAATCTGAG TTTTCTAAGG 
CTCTTCAGAG CTGCTCGTCT CATTAAGTTG CTCAGGCAGG GTGAAACCAT ACGCATCCTG 
CTCTGGACTT TTGTCCAGTC TTTCAAGGCT CTGCCGTATG TGTGCCTACT CATCGCCATG 
CTGTTCTTCA TCTACGCCAT CATTGGCATG CAGCTGTTTG GGAACATTAA GATCGAGGAG 
AACAGTGACA GTGCCATTAC CCAGCACAAC AACTTCAGGA CTTTTTTCCA GGCCCTAATG 
CTGCTCTTTA GGAGTGCTAC AGGTGAGGCA TGGCATGACA TCATGCTGTC ATGTCTGGGA 
AAGAAGCCGT GTGATATTCT CTCTGATAAC CCGAAGCCAG AATGCGGGAG CGAGTTTGCC 
TACCTCTACT TTGTCTCCTT CATCTTCCTC TGTTCATTTC TGATGCTGAA TCTGTTCGTG 
GCTGTGATCA TGGATAATTT TGAATACCTG ACGCGTGACT CCTCTATCCT GGGACCCCAT 
CACCTGGACG AGTATGTGAG GATCTGGGCA GAATATGATC CTGCTGCTTG CGGGCGCATT 
CATTATAAGG ATATGTACAG TTTATTGCGA GTTATCGACC CCCCTCTGGG CTTAGGCAAG 
AAATGTCCTC ATAGGGTGGC CTGCAAGAGA CTGTTGCGGA TGGATCTGCC GGTTGCCGAT 
GACAACACAG TGCACTTTAA TTCTACCCTT ATGGCCTTAA TCCGAACCGC TCTGGACATC 
AAGATCGCCA AGGGTGGGGA AGGAGGTGTA GATAAGCACC AGATGGATGC TGAATTAAGG 
AAGGAGATGA TGGCGATATG GCCTAATCTC TCTCAGAAGA ACCTGGACCT GCTGGTGACT 
CCTCACAAGT CAGCGACAGA CCTGACAGTA GGAAAGATCT ACGCTGCCAT GATGATCATG 
GAGTATTATC GGCAAAGTAA AGCCAAACGG ACACAAGCTC TCCATGATGA GCAGAATCGA 
ACGCCATTAA TGTTTCAGCG TCTGGAGCCG CCATCTCCTA GCCAGGATGT GGGACCAGGA 
CTCACTGGCC TTCCTGACAC ACAAATGCAC CCTGCAAATC ATCTGCCTGT TGATGAAAGG 
ATCCCAGAGA GCCAATCATG GGTGACAGCG CGAGCACAGG AGATTTCTCA AAAAGCAGGC 
AACTGGAGTC CAGAGGGCCA ACATCCAGAT GACACCACAG ACAACAGAAG GCAGTCTCAG 
ACAGTAGAGA TGAGAGAGAT GGGGCGGGAC GGGTACTCGG ACACAGAGCA ATATCTGCCG 
ATGGAAGGCC ATGGCAGGGC TGCTTCCATG CCTCGACTCC CAGCAGACAA CCAACAGCCA 
CGGAGGAAAG GGCGTCACCG GGGAGAAAAT CTCACTCAGC CTATCACAGA TAACAGTCCT 
ATGAAGCGCT CGGCTTCATC TTTGGGACAC GGGCGTGCAG GACGCTCCAT GCGAGGTGAG 
GATTATGCCA TGGAGAGGGT TATCCCAGAA GAGGGTCACA GGCATGGGCA TCGCCACCGA 
GACCGTAGTC ACCGGGCGTC AGAACGCTCC CTGAGCCGAT ACACTGATGC TGACACAGGT 
CTTGGAACTG ACCTCAGCAC AACCACACAA TCAGGAGACC TGCCATCCAA GGAGAGAGAC 
CGGGGCCGAG CCAAAGACCG CAAGCACCAT CATCATCATC ATCACCACCA CGGCTCTTTA 
GATAAGGAGC ACTACGGTCA TGAGAGAGAC AGAGGAGAGT ACGGCCACAG ACAGTCCAGA 
GAGAGAGACC GCCGCTGGTC CCGCTCACCC AGTGAGGGCA GAGAGTGTCT GACTCACAGA 
CAGGGCAGTA GTTCAGTAAG CGGCAGTCCA GTCCCGTCCA CCTCCGGGAC CAGCACCCCC 
CGCCGTGGCC GCCGTCAGCT CCCCCAAACC CCTGCTACAC CCCGGCCACA TGTCACCTAC 
TCACCTGTGG TCCGTAAGCC CATCAGCAGT ACCCCCCCTC CTGGCCAGCA GAGCCGACTC 
CCTACCCCGA CCTCCCGGCG CTTCAGTCCG ACTGGCCCCG AGCCCCCGCT TCCCCCTCAT 
CACCACCCGT CACCACCCCA CCACGGCTCT CCCCGCTCCG GCCGACATGC ACACTGGGGC 
CCCGAGCCTG CAGAGAGCGT GGAGGGTGAC GGCTTCTATG ACGACCAAGA CTATGAGTTC 
AATCACCACG AGCCTCCATC CTACGAGCAA AGCCCTGTGC AGGGCAACCC TCACCCCCAT 
TCCCCTAGGA CTTCCCGCCA CAACACCCCT CCTCAGGGCC AAGCGCACCC ACGGCGCATG 
CCAAATGGCT ACCGTTCGTC TTCGCCCTCT CCACACCACC ACGCGCCCGC TCACCCTGGA 
ACTCATAAAC CTCCTCATCC CAGAGGGCCC AGAAAGGGCC TCCATGAACC CTACAGTGAG 
ACTGACGAGG ATGACTGGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_021474398.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330073.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X9, mRNA.

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CloneID	ODa141791
Clone ID Related Accession (Same CDS sequence)	XM_021474398.1
Accession Version	XM_021474398.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGTGGCAGAA TCAGTTACAG GGATATGTAT GAGATGCTCA GGCACATGTC TCCTCCGTTA 
GGCCTGGGGA AGAAGTGCCC TGCACGGGTT GCCTACAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGAGGT GTAGATAAGC ACCAGATGGA TGCTGAATTA 
AGGAAGGAGA TGATGGCGAT ATGGCCTAAT CTCTCTCAGA AGAACCTGGA CCTGCTGGTG 
ACTCCTCACA AGTCGACAGA CCTGACAGTA GGAAAGATCT ACGCTGCCAT GATGATCATG 
GAGTATTATC GGCAAAGTAA AGCCAAACGG ACACAAGCTC TCCATGATGA GCAGAATCGA 
ACGCCATTAA TGTTTCAGCG TCTGGAGCCG CCATCTCCTA GCCAGGATGT GGGACCAGGA 
CTCACTGGCC TTCCTGACAC ACAAATGCAC CCTGCAAATC ATCTGCCTGT TGATGAAAGG 
ATCCCAGAGA GCCAATCATG GGTGACAGCG CGAGCACAGG AGATTTCTCA AAAAGCAGGC 
AACTGGAGTC CAGAGGGCCA ACATCCAGAT GACACCACAG ACAACAGAAG GCAGTCTCAG 
ACAGTAGAGA TGAGAGAGAT GGGGCGGGAC GGGTACTCGG ACACAGAGCA ATATCTGCCG 
ATGGAAGGCC ATGGCAGGGC TGCTTCCATG CCTCGACTCC CAGCAGACAA CCAACAGCCA 
CGGAGGAAAG GGCGTCACCG GGGAGAAAAT CTCACTCAGC CTATCACAGA TAACAGTCCT 
ATGAAGCGCT CGGCTTCATC TTTGGGACAC GGGCGTGCAG GACGCTCCAT GCGAGGTGAG 
GATTATGCCA TGGAGAGGGT TATCCCAGAA GAGGGTCACA GGCATGGGCA TCGCCACCGA 
GACCGTAGTC ACCGGGCGTC AGAACGCTCC CTGAGCCGAT ACACTGATGC TGACACAGGT 
CTTGGAACTG ACCTCAGCAC AACCACACAA TCAGGAGACC TGCCATCCAA GGAGAGAGAC 
CGGGGCCGAG CCAAAGACCG CAAGCACCAT CATCATCATC ATCACCACCA CGGCTCTTTA 
GATAAGGAGC ACTACGGTCA TGAGAGAGAC AGAGGAGAGT ACGGCCACAG ACAGTCCAGA 
GAGAGAGACC GCCGCTGGTC CCGCTCACCC AGTGAGGGCA GAGAGTGTCT GACTCACAGA 
CAGGGCAGTA GTTCAGTAAG CGGCAGTCCA GTCCCGTCCA CCTCCGGGAC CAGCACCCCC 
CGCCGTGGCC GCCGTCAGCT CCCCCAAACC CCTGCTACAC CCCGGCCACA TGTCACCTAC 
TCACCTGTGG TCCGTAAGCC CATCAGCAGT ACCCCCCCTC CTGGCCAGCA GAGCCGACTC 
CCTACCCCGA CCTCCCGGCG CTTCAGTCCG ACTGGCCCCG AGCCCCCGCT TCCCCCTCAT 
CACCACCCGT CACCACCCCA CCACGGCTCT CCCCGCTCCG GCCGACATGC ACACTGGGGC 
CCCGAGCCTG CAGAGAGCGT GGAGGGTGAC GGCTTCTATG ACGACCAAGA CTATGAGTTC 
AATCACCACG AGCCTCCATC CTACGAGCAA AGCCCTGTGC AGGGCAACCC TCACCCCCAT 
TCCCCTAGGA CTTCCCGCCA CAACACCCCT CCTCAGGGCC AAGCGCACCC ACGGCGCATG 
CCAAATGGCT ACCGTTCGTC TTCGCCCTCT CCACACCACC ACGCGCCCGC TCACCCTGGA 
ACTCATAAAC CTCCTCATCC CAGAGGGCCC AGAAAGGGCC TCCATGAACC CTACAGTGAG 
ACTGACGAGG ATGACTGGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330073.1
CDS	1510..8613
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGGSGGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGMNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRISYRDMYEMLRHMSPPLGLGKKCPARVAYKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQNRTPLMFQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQTVEMREMGRDGYSDTEQYLPMEGHGRAASMPRLPADNQQPRRKGRHRGENLTQPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_021474398.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474398.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGTGGCAGAA TCAGTTACAG GGATATGTAT GAGATGCTCA GGCACATGTC TCCTCCGTTA 
GGCCTGGGGA AGAAGTGCCC TGCACGGGTT GCCTACAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGAGGT GTAGATAAGC ACCAGATGGA TGCTGAATTA 
AGGAAGGAGA TGATGGCGAT ATGGCCTAAT CTCTCTCAGA AGAACCTGGA CCTGCTGGTG 
ACTCCTCACA AGTCGACAGA CCTGACAGTA GGAAAGATCT ACGCTGCCAT GATGATCATG 
GAGTATTATC GGCAAAGTAA AGCCAAACGG ACACAAGCTC TCCATGATGA GCAGAATCGA 
ACGCCATTAA TGTTTCAGCG TCTGGAGCCG CCATCTCCTA GCCAGGATGT GGGACCAGGA 
CTCACTGGCC TTCCTGACAC ACAAATGCAC CCTGCAAATC ATCTGCCTGT TGATGAAAGG 
ATCCCAGAGA GCCAATCATG GGTGACAGCG CGAGCACAGG AGATTTCTCA AAAAGCAGGC 
AACTGGAGTC CAGAGGGCCA ACATCCAGAT GACACCACAG ACAACAGAAG GCAGTCTCAG 
ACAGTAGAGA TGAGAGAGAT GGGGCGGGAC GGGTACTCGG ACACAGAGCA ATATCTGCCG 
ATGGAAGGCC ATGGCAGGGC TGCTTCCATG CCTCGACTCC CAGCAGACAA CCAACAGCCA 
CGGAGGAAAG GGCGTCACCG GGGAGAAAAT CTCACTCAGC CTATCACAGA TAACAGTCCT 
ATGAAGCGCT CGGCTTCATC TTTGGGACAC GGGCGTGCAG GACGCTCCAT GCGAGGTGAG 
GATTATGCCA TGGAGAGGGT TATCCCAGAA GAGGGTCACA GGCATGGGCA TCGCCACCGA 
GACCGTAGTC ACCGGGCGTC AGAACGCTCC CTGAGCCGAT ACACTGATGC TGACACAGGT 
CTTGGAACTG ACCTCAGCAC AACCACACAA TCAGGAGACC TGCCATCCAA GGAGAGAGAC 
CGGGGCCGAG CCAAAGACCG CAAGCACCAT CATCATCATC ATCACCACCA CGGCTCTTTA 
GATAAGGAGC ACTACGGTCA TGAGAGAGAC AGAGGAGAGT ACGGCCACAG ACAGTCCAGA 
GAGAGAGACC GCCGCTGGTC CCGCTCACCC AGTGAGGGCA GAGAGTGTCT GACTCACAGA 
CAGGGCAGTA GTTCAGTAAG CGGCAGTCCA GTCCCGTCCA CCTCCGGGAC CAGCACCCCC 
CGCCGTGGCC GCCGTCAGCT CCCCCAAACC CCTGCTACAC CCCGGCCACA TGTCACCTAC 
TCACCTGTGG TCCGTAAGCC CATCAGCAGT ACCCCCCCTC CTGGCCAGCA GAGCCGACTC 
CCTACCCCGA CCTCCCGGCG CTTCAGTCCG ACTGGCCCCG AGCCCCCGCT TCCCCCTCAT 
CACCACCCGT CACCACCCCA CCACGGCTCT CCCCGCTCCG GCCGACATGC ACACTGGGGC 
CCCGAGCCTG CAGAGAGCGT GGAGGGTGAC GGCTTCTATG ACGACCAAGA CTATGAGTTC 
AATCACCACG AGCCTCCATC CTACGAGCAA AGCCCTGTGC AGGGCAACCC TCACCCCCAT 
TCCCCTAGGA CTTCCCGCCA CAACACCCCT CCTCAGGGCC AAGCGCACCC ACGGCGCATG 
CCAAATGGCT ACCGTTCGTC TTCGCCCTCT CCACACCACC ACGCGCCCGC TCACCCTGGA 
ACTCATAAAC CTCCTCATCC CAGAGGGCCC AGAAAGGGCC TCCATGAACC CTACAGTGAG 
ACTGACGAGG ATGACTGGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_021474403.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330078.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X10, mRNA.

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CloneID	ODa141792
Clone ID Related Accession (Same CDS sequence)	XM_021474403.1
Accession Version	XM_021474403.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA ACAACTTCAT AAATCTGAGT 
TTTCTAAGGC TCTTCAGAGC TGCTCGTCTC ATTAAGTTGC TCAGGCAGGG TGAAACCATA 
CGCATCCTGC TCTGGACTTT TGTCCAGTCT TTCAAGGCTC TGCCGTATGT GTGCCTACTC 
ATCGCCATGC TGTTCTTCAT CTACGCCATC ATTGGCATGC AGCTGTTTGG GAACATTAAG 
ATCGAGGAGA ACAGTGACAG TGCCATTACC CAGCACAACA ACTTCAGGAC TTTTTTCCAG 
GCCCTAATGC TGCTCTTTAG GAGTGCTACA GGTGAGGCAT GGCATGACAT CATGCTGTCA 
TGTCTGGGAA AGAAGCCGTG TGATATTCTC TCTGATAACC CGAAGCCAGA ATGCGGGAGC 
GAGTTTGCCT ACCTCTACTT TGTCTCCTTC ATCTTCCTCT GTTCATTTCT GATGCTGAAT 
CTGTTCGTGG CTGTGATCAT GGATAATTTT GAATACCTGA CGCGTGACTC CTCTATCCTG 
GGACCCCATC ACCTGGACGA GTATGTGAGG ATCTGGGCAG AATATGATCC TGCTGCTTGT 
GGCAGAATCA GTTACAGGGA TATGTATGAG ATGCTCAGGC ACATGTCTCC TCCGTTAGGC 
CTGGGGAAGA AGTGCCCTGC ACGGGTTGCC TACAAGAGAC TGTTGCGGAT GGATCTGCCG 
GTTGCCGATG ACAACACAGT GCACTTTAAT TCTACCCTTA TGGCCTTAAT CCGAACCGCT 
CTGGACATCA AGATCGCCAA GGGAGGTGTA GATAAGCACC AGATGGATGC TGAATTAAGG 
AAGGAGATGA TGGCGATATG GCCTAATCTC TCTCAGAAGA ACCTGGACCT GCTGGTGACT 
CCTCACAAGT CGACAGACCT GACAGTAGGA AAGATCTACG CTGCCATGAT GATCATGGAG 
TATTATCGGC AAAGTAAAGC CAAACGGACA CAAGCTCTCC ATGATGAGCA GAATCGAACG 
CCATTAATGT TTCAGCGTCT GGAGCCGCCA TCTCCTAGCC AGGATGTGGG ACCAGGACTC 
ACTGGCCTTC CTGACACACA AATGCACCCT GCAAATCATC TGCCTGTTGA TGAAAGGATC 
CCAGAGAGCC AATCATGGGT GACAGCGCGA GCACAGGAGA TTTCTCAAAA AGCAGGCAAC 
TGGAGTCCAG AGGGCCAACA TCCAGATGAC ACCACAGACA ACAGAAGGCA GTCTCAGACA 
GTAGAGATGA GAGAGATGGG GCGGGACGGG TACTCGGACA CAGAGCAATA TCTGCCGATG 
GAAGGCCATG GCAGGGCTGC TTCCATGCCT CGACTCCCAG CAGACAACCA ACAGCCACGG 
AGGAAAGGGC GTCACCGGGG AGAAAATCTC ACTCAGCCTA TCACAGATAA CAGTCCTATG 
AAGCGCTCGG CTTCATCTTT GGGACACGGG CGTGCAGGAC GCTCCATGCG AGGTGAGGAT 
TATGCCATGG AGAGGGTTAT CCCAGAAGAG GGTCACAGGC ATGGGCATCG CCACCGAGAC 
CGTAGTCACC GGGCGTCAGA ACGCTCCCTG AGCCGATACA CTGATGCTGA CACAGGTCTT 
GGAACTGACC TCAGCACAAC CACACAATCA GGAGACCTGC CATCCAAGGA GAGAGACCGG 
GGCCGAGCCA AAGACCGCAA GCACCATCAT CATCATCATC ACCACCACGG CTCTTTAGAT 
AAGGAGCACT ACGGTCATGA GAGAGACAGA GGAGAGTACG GCCACAGACA GTCCAGAGAG 
AGAGACCGCC GCTGGTCCCG CTCACCCAGT GAGGGCAGAG AGTGTCTGAC TCACAGACAG 
GGCAGTAGTT CAGTAAGCGG CAGTCCAGTC CCGTCCACCT CCGGGACCAG CACCCCCCGC 
CGTGGCCGCC GTCAGCTCCC CCAAACCCCT GCTACACCCC GGCCACATGT CACCTACTCA 
CCTGTGGTCC GTAAGCCCAT CAGCAGTACC CCCCCTCCTG GCCAGCAGAG CCGACTCCCT 
ACCCCGACCT CCCGGCGCTT CAGTCCGACT GGCCCCGAGC CCCCGCTTCC CCCTCATCAC 
CACCCGTCAC CACCCCACCA CGGCTCTCCC CGCTCCGGCC GACATGCACA CTGGGGCCCC 
GAGCCTGCAG AGAGCGTGGA GGGTGACGGC TTCTATGACG ACCAAGACTA TGAGTTCAAT 
CACCACGAGC CTCCATCCTA CGAGCAAAGC CCTGTGCAGG GCAACCCTCA CCCCCATTCC 
CCTAGGACTT CCCGCCACAA CACCCCTCCT CAGGGCCAAG CGCACCCACG GCGCATGCCA 
AATGGCTACC GTTCGTCTTC GCCCTCTCCA CACCACCACG CGCCCGCTCA CCCTGGAACT 
CATAAACCTC CTCATCCCAG AGGGCCCAGA AAGGGCCTCC ATGAACCCTA CAGTGAGACT 
GACGAGGATG ACTGGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330078.1
CDS	1510..8610
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGGSGGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRISYRDMYEMLRHMSPPLGLGKKCPARVAYKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQNRTPLMFQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQTVEMREMGRDGYSDTEQYLPMEGHGRAASMPRLPADNQQPRRKGRHRGENLTQPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_021474403.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474403.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA ACAACTTCAT AAATCTGAGT 
TTTCTAAGGC TCTTCAGAGC TGCTCGTCTC ATTAAGTTGC TCAGGCAGGG TGAAACCATA 
CGCATCCTGC TCTGGACTTT TGTCCAGTCT TTCAAGGCTC TGCCGTATGT GTGCCTACTC 
ATCGCCATGC TGTTCTTCAT CTACGCCATC ATTGGCATGC AGCTGTTTGG GAACATTAAG 
ATCGAGGAGA ACAGTGACAG TGCCATTACC CAGCACAACA ACTTCAGGAC TTTTTTCCAG 
GCCCTAATGC TGCTCTTTAG GAGTGCTACA GGTGAGGCAT GGCATGACAT CATGCTGTCA 
TGTCTGGGAA AGAAGCCGTG TGATATTCTC TCTGATAACC CGAAGCCAGA ATGCGGGAGC 
GAGTTTGCCT ACCTCTACTT TGTCTCCTTC ATCTTCCTCT GTTCATTTCT GATGCTGAAT 
CTGTTCGTGG CTGTGATCAT GGATAATTTT GAATACCTGA CGCGTGACTC CTCTATCCTG 
GGACCCCATC ACCTGGACGA GTATGTGAGG ATCTGGGCAG AATATGATCC TGCTGCTTGT 
GGCAGAATCA GTTACAGGGA TATGTATGAG ATGCTCAGGC ACATGTCTCC TCCGTTAGGC 
CTGGGGAAGA AGTGCCCTGC ACGGGTTGCC TACAAGAGAC TGTTGCGGAT GGATCTGCCG 
GTTGCCGATG ACAACACAGT GCACTTTAAT TCTACCCTTA TGGCCTTAAT CCGAACCGCT 
CTGGACATCA AGATCGCCAA GGGAGGTGTA GATAAGCACC AGATGGATGC TGAATTAAGG 
AAGGAGATGA TGGCGATATG GCCTAATCTC TCTCAGAAGA ACCTGGACCT GCTGGTGACT 
CCTCACAAGT CGACAGACCT GACAGTAGGA AAGATCTACG CTGCCATGAT GATCATGGAG 
TATTATCGGC AAAGTAAAGC CAAACGGACA CAAGCTCTCC ATGATGAGCA GAATCGAACG 
CCATTAATGT TTCAGCGTCT GGAGCCGCCA TCTCCTAGCC AGGATGTGGG ACCAGGACTC 
ACTGGCCTTC CTGACACACA AATGCACCCT GCAAATCATC TGCCTGTTGA TGAAAGGATC 
CCAGAGAGCC AATCATGGGT GACAGCGCGA GCACAGGAGA TTTCTCAAAA AGCAGGCAAC 
TGGAGTCCAG AGGGCCAACA TCCAGATGAC ACCACAGACA ACAGAAGGCA GTCTCAGACA 
GTAGAGATGA GAGAGATGGG GCGGGACGGG TACTCGGACA CAGAGCAATA TCTGCCGATG 
GAAGGCCATG GCAGGGCTGC TTCCATGCCT CGACTCCCAG CAGACAACCA ACAGCCACGG 
AGGAAAGGGC GTCACCGGGG AGAAAATCTC ACTCAGCCTA TCACAGATAA CAGTCCTATG 
AAGCGCTCGG CTTCATCTTT GGGACACGGG CGTGCAGGAC GCTCCATGCG AGGTGAGGAT 
TATGCCATGG AGAGGGTTAT CCCAGAAGAG GGTCACAGGC ATGGGCATCG CCACCGAGAC 
CGTAGTCACC GGGCGTCAGA ACGCTCCCTG AGCCGATACA CTGATGCTGA CACAGGTCTT 
GGAACTGACC TCAGCACAAC CACACAATCA GGAGACCTGC CATCCAAGGA GAGAGACCGG 
GGCCGAGCCA AAGACCGCAA GCACCATCAT CATCATCATC ACCACCACGG CTCTTTAGAT 
AAGGAGCACT ACGGTCATGA GAGAGACAGA GGAGAGTACG GCCACAGACA GTCCAGAGAG 
AGAGACCGCC GCTGGTCCCG CTCACCCAGT GAGGGCAGAG AGTGTCTGAC TCACAGACAG 
GGCAGTAGTT CAGTAAGCGG CAGTCCAGTC CCGTCCACCT CCGGGACCAG CACCCCCCGC 
CGTGGCCGCC GTCAGCTCCC CCAAACCCCT GCTACACCCC GGCCACATGT CACCTACTCA 
CCTGTGGTCC GTAAGCCCAT CAGCAGTACC CCCCCTCCTG GCCAGCAGAG CCGACTCCCT 
ACCCCGACCT CCCGGCGCTT CAGTCCGACT GGCCCCGAGC CCCCGCTTCC CCCTCATCAC 
CACCCGTCAC CACCCCACCA CGGCTCTCCC CGCTCCGGCC GACATGCACA CTGGGGCCCC 
GAGCCTGCAG AGAGCGTGGA GGGTGACGGC TTCTATGACG ACCAAGACTA TGAGTTCAAT 
CACCACGAGC CTCCATCCTA CGAGCAAAGC CCTGTGCAGG GCAACCCTCA CCCCCATTCC 
CCTAGGACTT CCCGCCACAA CACCCCTCCT CAGGGCCAAG CGCACCCACG GCGCATGCCA 
AATGGCTACC GTTCGTCTTC GCCCTCTCCA CACCACCACG CGCCCGCTCA CCCTGGAACT 
CATAAACCTC CTCATCCCAG AGGGCCCAGA AAGGGCCTCC ATGAACCCTA CAGTGAGACT 
GACGAGGATG ACTGGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_021474404.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330079.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X11, mRNA.

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CloneID	ODa141793
Clone ID Related Accession (Same CDS sequence)	XM_021474404.1
Accession Version	XM_021474404.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG 
GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT 
GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC 
CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC 
ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT 
GAGCAGCGTC TGGAGCCGCC ATCTCCTAGC CAGGATGTGG GACCAGGACT CACTGGCCTT 
CCTGACACAC AAATGCACCC TGCAAATCAT CTGCCTGTTG ATGAAAGGAT CCCAGAGAGC 
CAATCATGGG TGACAGCGCG AGCACAGGAG ATTTCTCAAA AAGCAGGCAA CTGGAGTCCA 
GAGGGCCAAC ATCCAGATGA CACCACAGAC AACAGAAGGC AGTCTCAGAC AGTAGAGATG 
AGAGAGATGG GGCGGGACGG GTACTCGGAC ACAGAGCAAT ATCTGCCGAT GGAAGGCCAT 
GGCAGGGCTG CTTCCATGCC TCGACTCCCA GCAGACAACC AACAGCCACG GAGGAAAGGG 
CGTCACCGGG GAGAAAATCT CACTCAGCCT ATCACAGATA ACAGTCCTAT GAAGCGCTCG 
GCTTCATCTT TGGGACACGG GCGTGCAGGA CGCTCCATGC GAGGTGAGGA TTATGCCATG 
GAGAGGGTTA TCCCAGAAGA GGGTCACAGG CATGGGCATC GCCACCGAGA CCGTAGTCAC 
CGGGCGTCAG AACGCTCCCT GAGCCGATAC ACTGATGCTG ACACAGGTCT TGGAACTGAC 
CTCAGCACAA CCACACAATC AGGAGACCTG CCATCCAAGG AGAGAGACCG GGGCCGAGCC 
AAAGACCGCA AGCACCATCA TCATCATCAT CACCACCACG GCTCTTTAGA TAAGGAGCAC 
TACGGTCATG AGAGAGACAG AGGAGAGTAC GGCCACAGAC AGTCCAGAGA GAGAGACCGC 
CGCTGGTCCC GCTCACCCAG TGAGGGCAGA GAGTGTCTGA CTCACAGACA GGGCAGTAGT 
TCAGTAAGCG GCAGTCCAGT CCCGTCCACC TCCGGGACCA GCACCCCCCG CCGTGGCCGC 
CGTCAGCTCC CCCAAACCCC TGCTACACCC CGGCCACATG TCACCTACTC ACCTGTGGTC 
CGTAAGCCCA TCAGCAGTAC CCCCCCTCCT GGCCAGCAGA GCCGACTCCC TACCCCGACC 
TCCCGGCGCT TCAGTCCGAC TGGCCCCGAG CCCCCGCTTC CCCCTCATCA CCACCCGTCA 
CCACCCCACC ACGGCTCTCC CCGCTCCGGC CGACATGCAC ACTGGGGCCC CGAGCCTGCA 
GAGAGCGTGG AGGGTGACGG CTTCTATGAC GACCAAGACT ATGAGTTCAA TCACCACGAG 
CCTCCATCCT ACGAGCAAAG CCCTGTGCAG GGCAACCCTC ACCCCCATTC CCCTAGGACT 
TCCCGCCACA ACACCCCTCC TCAGGGCCAA GCGCACCCAC GGCGCATGCC AAATGGCTAC 
CGTTCGTCTT CGCCCTCTCC ACACCACCAC GCGCCCGCTC ACCCTGGAAC TCATAAACCT 
CCTCATCCCA GAGGGCCCAG AAAGGGCCTC CATGAACCCT ACAGTGAGAC TGACGAGGAT 
GACTGGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330079.1
CDS	1508..8599
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGGSGGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGMNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGEGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQTVEMREMGRDGYSDTEQYLPMEGHGRAASMPRLPADNQQPRRKGRHRGENLTQPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_021474404.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474404.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG 
GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT 
GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC 
CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC 
ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT 
GAGCAGCGTC TGGAGCCGCC ATCTCCTAGC CAGGATGTGG GACCAGGACT CACTGGCCTT 
CCTGACACAC AAATGCACCC TGCAAATCAT CTGCCTGTTG ATGAAAGGAT CCCAGAGAGC 
CAATCATGGG TGACAGCGCG AGCACAGGAG ATTTCTCAAA AAGCAGGCAA CTGGAGTCCA 
GAGGGCCAAC ATCCAGATGA CACCACAGAC AACAGAAGGC AGTCTCAGAC AGTAGAGATG 
AGAGAGATGG GGCGGGACGG GTACTCGGAC ACAGAGCAAT ATCTGCCGAT GGAAGGCCAT 
GGCAGGGCTG CTTCCATGCC TCGACTCCCA GCAGACAACC AACAGCCACG GAGGAAAGGG 
CGTCACCGGG GAGAAAATCT CACTCAGCCT ATCACAGATA ACAGTCCTAT GAAGCGCTCG 
GCTTCATCTT TGGGACACGG GCGTGCAGGA CGCTCCATGC GAGGTGAGGA TTATGCCATG 
GAGAGGGTTA TCCCAGAAGA GGGTCACAGG CATGGGCATC GCCACCGAGA CCGTAGTCAC 
CGGGCGTCAG AACGCTCCCT GAGCCGATAC ACTGATGCTG ACACAGGTCT TGGAACTGAC 
CTCAGCACAA CCACACAATC AGGAGACCTG CCATCCAAGG AGAGAGACCG GGGCCGAGCC 
AAAGACCGCA AGCACCATCA TCATCATCAT CACCACCACG GCTCTTTAGA TAAGGAGCAC 
TACGGTCATG AGAGAGACAG AGGAGAGTAC GGCCACAGAC AGTCCAGAGA GAGAGACCGC 
CGCTGGTCCC GCTCACCCAG TGAGGGCAGA GAGTGTCTGA CTCACAGACA GGGCAGTAGT 
TCAGTAAGCG GCAGTCCAGT CCCGTCCACC TCCGGGACCA GCACCCCCCG CCGTGGCCGC 
CGTCAGCTCC CCCAAACCCC TGCTACACCC CGGCCACATG TCACCTACTC ACCTGTGGTC 
CGTAAGCCCA TCAGCAGTAC CCCCCCTCCT GGCCAGCAGA GCCGACTCCC TACCCCGACC 
TCCCGGCGCT TCAGTCCGAC TGGCCCCGAG CCCCCGCTTC CCCCTCATCA CCACCCGTCA 
CCACCCCACC ACGGCTCTCC CCGCTCCGGC CGACATGCAC ACTGGGGCCC CGAGCCTGCA 
GAGAGCGTGG AGGGTGACGG CTTCTATGAC GACCAAGACT ATGAGTTCAA TCACCACGAG 
CCTCCATCCT ACGAGCAAAG CCCTGTGCAG GGCAACCCTC ACCCCCATTC CCCTAGGACT 
TCCCGCCACA ACACCCCTCC TCAGGGCCAA GCGCACCCAC GGCGCATGCC AAATGGCTAC 
CGTTCGTCTT CGCCCTCTCC ACACCACCAC GCGCCCGCTC ACCCTGGAAC TCATAAACCT 
CCTCATCCCA GAGGGCCCAG AAAGGGCCTC CATGAACCCT ACAGTGAGAC TGACGAGGAT 
GACTGGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_021474408.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330083.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X12, mRNA.

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CloneID	ODa141794
Clone ID Related Accession (Same CDS sequence)	XM_021474408.1
Accession Version	XM_021474408.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACAGGAAGC AGCAAAGGGA AAGACATCAG TACTATCAAG 
TCTCTACGTG TGCTTCGAGT ACTGCGGCCG CTGAAAACCA TCAAACGTCT GCCAAAACTG 
AAGGCTGTCT TTGACTGTGT GGTGAACTCT CTGAAGAATG TGTTGAACAT CCTCATCGTC 
TACATGCTCT TCATGTTCAT CTTCGCTGTG GTGGCTGTGC AGCTCTTTAA GGGACGTTTC 
TTTTACTGCA CTGATGAATC CAAAGAGTTT GAGCGCGACT GCAGGGGCGA GTATCTGGTC 
TATGAAAGAG ATAACGAAGT GAGGTCACAG AAGCGGGAAT GGAAGAAATA CGATTTCCAC 
TACGACAACG TGCTCTGGGC CCTTCTCACC CTCTTCACCG TTTCTACGGG AGAGGGGTGG 
CCGCAAGTGC TAAAACATTC AGTAGATGCG ACCTACGAGA ATCAGGGCCC GAGCCCAGGT 
TACCGGATGG AAATGTCCAT CTTTTACGTG GTGTACTTCG TGGTCTTCCC CTTCTTCTTC 
GTCAACATCT TCGTGGCTCT TATCATCATT ACTTTCCAGG AGCAAGGAGA CAAAATGATG 
GAGGACTATA GCCTGGAAAA GAATGAGAGA GCCTGCATAG ACTTTGCCAT CAACGCTAAG 
CCGCTCACAC GGCACATGCC CCAGAACAAG CAGACCTTTC AGTACCGCAT GTGGGAGTTT 
GTGGTGTCTC CTCCATTTGA ATACACCATC ATGGCCTTGA TCGCGCTCAA CACTATTGTC 
CTTATGATGA AGTATGATGG AGCATCGCTT ACTTATGAAG ATGTGCTAAA ATACCTGAAC 
ATAGTGTTCA CCTCTCTATT CTCCATGGAG TGCATATTGA AGATCATAGC CTTCGGAGCA 
TTGAACTACT TCAAAGATGC CTGGAACATC TTCGACTGTG TGACTGTACT TGGCAGCATC 
ACTGATATTT TAGTCACAGA GCTGGGGAAC AACTTCATAA ATCTGAGTTT TCTAAGGCTC 
TTCAGAGCTG CTCGTCTCAT TAAGTTGCTC AGGCAGGGTG AAACCATACG CATCCTGCTC 
TGGACTTTTG TCCAGTCTTT CAAGGCTCTG CCGTATGTGT GCCTACTCAT CGCCATGCTG 
TTCTTCATCT ACGCCATCAT TGGCATGCAG CTGTTTGGGA ACATTAAGAT CGAGGAGAAC 
AGTGACAGTG CCATTACCCA GCACAACAAC TTCAGGACTT TTTTCCAGGC CCTAATGCTG 
CTCTTTAGGA GTGCTACAGG TGAGGCATGG CATGACATCA TGCTGTCATG TCTGGGAAAG 
AAGCCGTGTG ATATTCTCTC TGATAACCCG AAGCCAGAAT GCGGGAGCGA GTTTGCCTAC 
CTCTACTTTG TCTCCTTCAT CTTCCTCTGT TCATTTCTGA TGCTGAATCT GTTCGTGGCT 
GTGATCATGG ATAATTTTGA ATACCTGACG CGTGACTCCT CTATCCTGGG ACCCCATCAC 
CTGGACGAGT ATGTGAGGAT CTGGGCAGAA TATGATCCTG CTGCTTGCGG GCGCATTCAT 
TATAAGGATA TGTACAGTTT ATTGCGAGTT ATCGACCCCC CTCTGGGCTT AGGCAAGAAA 
TGTCCTCATA GGGTGGCCTG CAAGAGACTG TTGCGGATGG ATCTGCCGGT TGCCGATGAC 
AACACAGTGC ACTTTAATTC TACCCTTATG GCCTTAATCC GAACCGCTCT GGACATCAAG 
ATCGCCAAGG GAGGTGTAGA TAAGCACCAG ATGGATGCTG AATTAAGGAA GGAGATGATG 
GCGATATGGC CTAATCTCTC TCAGAAGAAC CTGGACCTGC TGGTGACTCC TCACAAGTCG 
ACAGACCTGA CAGTAGGAAA GATCTACGCT GCCATGATGA TCATGGAGTA TTATCGGCAA 
AGTAAAGCCA AACGGACACA AGCTCTCCAT GATGAGCAGA ATCGAACGCC ATTAATGTTT 
CAGCGTCTGG AGCCGCCATC TCCTAGCCAG GATGTGGGAC CAGGACTCAC TGGCCTTCCT 
GACACACAAA TGCACCCTGC AAATCATCTG CCTGTTGATG AAAGGATCCC AGAGAGCCAA 
TCATGGGTGA CAGCGCGAGC ACAGGAGATT TCTCAAAAAG CAGGCAACTG GAGTCCAGAG 
GGCCAACATC CAGATGACAC CACAGACAAC AGAAGGCAGT CTCAGACAGT AGAGATGAGA 
GAGATGGGGC GGGACGGGTA CTCGGACACA GAGCAATATC TGCCGATGGA AGGCCATGGC 
AGGGCTGCTT CCATGCCTCG ACTCCCAGCA GACAACCAAC AGCCACGGAG GAAAGGGCGT 
CACCGGGGAG AAAATCTCAC TCAGCCTATC ACAGATAACA GTCCTATGAA GCGCTCGGCT 
TCATCTTTGG GACACGGGCG TGCAGGACGC TCCATGCGAG GTGAGGATTA TGCCATGGAG 
AGGGTTATCC CAGAAGAGGG TCACAGGCAT GGGCATCGCC ACCGAGACCG TAGTCACCGG 
GCGTCAGAAC GCTCCCTGAG CCGATACACT GATGCTGACA CAGGTCTTGG AACTGACCTC 
AGCACAACCA CACAATCAGG AGACCTGCCA TCCAAGGAGA GAGACCGGGG CCGAGCCAAA 
GACCGCAAGC ACCATCATCA TCATCATCAC CACCACGGCT CTTTAGATAA GGAGCACTAC 
GGTCATGAGA GAGACAGAGG AGAGTACGGC CACAGACAGT CCAGAGAGAG AGACCGCCGC 
TGGTCCCGCT CACCCAGTGA GGGCAGAGAG TGTCTGACTC ACAGACAGGG CAGTAGTTCA 
GTAAGCGGCA GTCCAGTCCC GTCCACCTCC GGGACCAGCA CCCCCCGCCG TGGCCGCCGT 
CAGCTCCCCC AAACCCCTGC TACACCCCGG CCACATGTCA CCTACTCACC TGTGGTCCGT 
AAGCCCATCA GCAGTACCCC CCCTCCTGGC CAGCAGAGCC GACTCCCTAC CCCGACCTCC 
CGGCGCTTCA GTCCGACTGG CCCCGAGCCC CCGCTTCCCC CTCATCACCA CCCGTCACCA 
CCCCACCACG GCTCTCCCCG CTCCGGCCGA CATGCACACT GGGGCCCCGA GCCTGCAGAG 
AGCGTGGAGG GTGACGGCTT CTATGACGAC CAAGACTATG AGTTCAATCA CCACGAGCCT 
CCATCCTACG AGCAAAGCCC TGTGCAGGGC AACCCTCACC CCCATTCCCC TAGGACTTCC 
CGCCACAACA CCCCTCCTCA GGGCCAAGCG CACCCACGGC GCATGCCAAA TGGCTACCGT 
TCGTCTTCGC CCTCTCCACA CCACCACGCG CCCGCTCACC CTGGAACTCA TAAACCTCCT 
CATCCCAGAG GGCCCAGAAA GGGCCTCCAT GAACCCTACA GTGAGACTGA CGAGGATGAC 
TGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330083.1
CDS	1509..8597
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQNRTPLMFQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQTVEMREMGRDGYSDTEQYLPMEGHGRAASMPRLPADNQQPRRKGRHRGENLTQPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_021474408.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474408.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACAGGAAGC AGCAAAGGGA AAGACATCAG TACTATCAAG 
TCTCTACGTG TGCTTCGAGT ACTGCGGCCG CTGAAAACCA TCAAACGTCT GCCAAAACTG 
AAGGCTGTCT TTGACTGTGT GGTGAACTCT CTGAAGAATG TGTTGAACAT CCTCATCGTC 
TACATGCTCT TCATGTTCAT CTTCGCTGTG GTGGCTGTGC AGCTCTTTAA GGGACGTTTC 
TTTTACTGCA CTGATGAATC CAAAGAGTTT GAGCGCGACT GCAGGGGCGA GTATCTGGTC 
TATGAAAGAG ATAACGAAGT GAGGTCACAG AAGCGGGAAT GGAAGAAATA CGATTTCCAC 
TACGACAACG TGCTCTGGGC CCTTCTCACC CTCTTCACCG TTTCTACGGG AGAGGGGTGG 
CCGCAAGTGC TAAAACATTC AGTAGATGCG ACCTACGAGA ATCAGGGCCC GAGCCCAGGT 
TACCGGATGG AAATGTCCAT CTTTTACGTG GTGTACTTCG TGGTCTTCCC CTTCTTCTTC 
GTCAACATCT TCGTGGCTCT TATCATCATT ACTTTCCAGG AGCAAGGAGA CAAAATGATG 
GAGGACTATA GCCTGGAAAA GAATGAGAGA GCCTGCATAG ACTTTGCCAT CAACGCTAAG 
CCGCTCACAC GGCACATGCC CCAGAACAAG CAGACCTTTC AGTACCGCAT GTGGGAGTTT 
GTGGTGTCTC CTCCATTTGA ATACACCATC ATGGCCTTGA TCGCGCTCAA CACTATTGTC 
CTTATGATGA AGTATGATGG AGCATCGCTT ACTTATGAAG ATGTGCTAAA ATACCTGAAC 
ATAGTGTTCA CCTCTCTATT CTCCATGGAG TGCATATTGA AGATCATAGC CTTCGGAGCA 
TTGAACTACT TCAAAGATGC CTGGAACATC TTCGACTGTG TGACTGTACT TGGCAGCATC 
ACTGATATTT TAGTCACAGA GCTGGGGAAC AACTTCATAA ATCTGAGTTT TCTAAGGCTC 
TTCAGAGCTG CTCGTCTCAT TAAGTTGCTC AGGCAGGGTG AAACCATACG CATCCTGCTC 
TGGACTTTTG TCCAGTCTTT CAAGGCTCTG CCGTATGTGT GCCTACTCAT CGCCATGCTG 
TTCTTCATCT ACGCCATCAT TGGCATGCAG CTGTTTGGGA ACATTAAGAT CGAGGAGAAC 
AGTGACAGTG CCATTACCCA GCACAACAAC TTCAGGACTT TTTTCCAGGC CCTAATGCTG 
CTCTTTAGGA GTGCTACAGG TGAGGCATGG CATGACATCA TGCTGTCATG TCTGGGAAAG 
AAGCCGTGTG ATATTCTCTC TGATAACCCG AAGCCAGAAT GCGGGAGCGA GTTTGCCTAC 
CTCTACTTTG TCTCCTTCAT CTTCCTCTGT TCATTTCTGA TGCTGAATCT GTTCGTGGCT 
GTGATCATGG ATAATTTTGA ATACCTGACG CGTGACTCCT CTATCCTGGG ACCCCATCAC 
CTGGACGAGT ATGTGAGGAT CTGGGCAGAA TATGATCCTG CTGCTTGCGG GCGCATTCAT 
TATAAGGATA TGTACAGTTT ATTGCGAGTT ATCGACCCCC CTCTGGGCTT AGGCAAGAAA 
TGTCCTCATA GGGTGGCCTG CAAGAGACTG TTGCGGATGG ATCTGCCGGT TGCCGATGAC 
AACACAGTGC ACTTTAATTC TACCCTTATG GCCTTAATCC GAACCGCTCT GGACATCAAG 
ATCGCCAAGG GAGGTGTAGA TAAGCACCAG ATGGATGCTG AATTAAGGAA GGAGATGATG 
GCGATATGGC CTAATCTCTC TCAGAAGAAC CTGGACCTGC TGGTGACTCC TCACAAGTCG 
ACAGACCTGA CAGTAGGAAA GATCTACGCT GCCATGATGA TCATGGAGTA TTATCGGCAA 
AGTAAAGCCA AACGGACACA AGCTCTCCAT GATGAGCAGA ATCGAACGCC ATTAATGTTT 
CAGCGTCTGG AGCCGCCATC TCCTAGCCAG GATGTGGGAC CAGGACTCAC TGGCCTTCCT 
GACACACAAA TGCACCCTGC AAATCATCTG CCTGTTGATG AAAGGATCCC AGAGAGCCAA 
TCATGGGTGA CAGCGCGAGC ACAGGAGATT TCTCAAAAAG CAGGCAACTG GAGTCCAGAG 
GGCCAACATC CAGATGACAC CACAGACAAC AGAAGGCAGT CTCAGACAGT AGAGATGAGA 
GAGATGGGGC GGGACGGGTA CTCGGACACA GAGCAATATC TGCCGATGGA AGGCCATGGC 
AGGGCTGCTT CCATGCCTCG ACTCCCAGCA GACAACCAAC AGCCACGGAG GAAAGGGCGT 
CACCGGGGAG AAAATCTCAC TCAGCCTATC ACAGATAACA GTCCTATGAA GCGCTCGGCT 
TCATCTTTGG GACACGGGCG TGCAGGACGC TCCATGCGAG GTGAGGATTA TGCCATGGAG 
AGGGTTATCC CAGAAGAGGG TCACAGGCAT GGGCATCGCC ACCGAGACCG TAGTCACCGG 
GCGTCAGAAC GCTCCCTGAG CCGATACACT GATGCTGACA CAGGTCTTGG AACTGACCTC 
AGCACAACCA CACAATCAGG AGACCTGCCA TCCAAGGAGA GAGACCGGGG CCGAGCCAAA 
GACCGCAAGC ACCATCATCA TCATCATCAC CACCACGGCT CTTTAGATAA GGAGCACTAC 
GGTCATGAGA GAGACAGAGG AGAGTACGGC CACAGACAGT CCAGAGAGAG AGACCGCCGC 
TGGTCCCGCT CACCCAGTGA GGGCAGAGAG TGTCTGACTC ACAGACAGGG CAGTAGTTCA 
GTAAGCGGCA GTCCAGTCCC GTCCACCTCC GGGACCAGCA CCCCCCGCCG TGGCCGCCGT 
CAGCTCCCCC AAACCCCTGC TACACCCCGG CCACATGTCA CCTACTCACC TGTGGTCCGT 
AAGCCCATCA GCAGTACCCC CCCTCCTGGC CAGCAGAGCC GACTCCCTAC CCCGACCTCC 
CGGCGCTTCA GTCCGACTGG CCCCGAGCCC CCGCTTCCCC CTCATCACCA CCCGTCACCA 
CCCCACCACG GCTCTCCCCG CTCCGGCCGA CATGCACACT GGGGCCCCGA GCCTGCAGAG 
AGCGTGGAGG GTGACGGCTT CTATGACGAC CAAGACTATG AGTTCAATCA CCACGAGCCT 
CCATCCTACG AGCAAAGCCC TGTGCAGGGC AACCCTCACC CCCATTCCCC TAGGACTTCC 
CGCCACAACA CCCCTCCTCA GGGCCAAGCG CACCCACGGC GCATGCCAAA TGGCTACCGT 
TCGTCTTCGC CCTCTCCACA CCACCACGCG CCCGCTCACC CTGGAACTCA TAAACCTCCT 
CATCCCAGAG GGCCCAGAAA GGGCCTCCAT GAACCCTACA GTGAGACTGA CGAGGATGAC 
TGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_021474412.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330087.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X13, mRNA.

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CloneID	ODa141795
Clone ID Related Accession (Same CDS sequence)	XM_021474412.1
Accession Version	XM_021474412.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACAGGAAGC AGCAAAGGGA AAGACATCAG TACTATCAAG 
TCTCTACGTG TGCTTCGAGT ACTGCGGCCG CTGAAAACCA TCAAACGTCT GCCAAAACTG 
AAGGCTGTCT TTGACTGTGT GGTGAACTCT CTGAAGAATG TGTTGAACAT CCTCATCGTC 
TACATGCTCT TCATGTTCAT CTTCGCTGTG GTGGCTGTGC AGCTCTTTAA GGGACGTTTC 
TTTTACTGCA CTGATGAATC CAAAGAGTTT GAGCGCGACT GCAGGGGCGA GTATCTGGTC 
TATGAAAGAG ATAACGAAGT GAGGTCACAG AAGCGGGAAT GGAAGAAATA CGATTTCCAC 
TACGACAACG TGCTCTGGGC CCTTCTCACC CTCTTCACCG TTTCTACGGG AGAGGGGTGG 
CCGCAAGTGC TAAAACATTC AGTAGATGCG ACCTACGAGA ATCAGGGCCC GAGCCCAGGT 
TACCGGATGG AAATGTCCAT CTTTTACGTG GTGTACTTCG TGGTCTTCCC CTTCTTCTTC 
GTCAACATCT TCGTGGCTCT TATCATCATT ACTTTCCAGG AGCAAGGAGA CAAAATGATG 
GAGGACTATA GCCTGGAAAA GAATGAGAGA GCCTGCATAG ACTTTGCCAT CAACGCTAAG 
CCGCTCACAC GGCACATGCC CCAGAACAAG CAGACCTTTC AGTACCGCAT GTGGGAGTTT 
GTGGTGTCTC CTCCATTTGA ATACACCATC ATGGCCTTGA TCGCGCTCAA CACTATTGTC 
CTTATGATGA AGTATGATGG AGCATCGCTT ACTTATGAAG ATGTGCTAAA ATACCTGAAC 
ATAGTGTTCA CCTCTCTATT CTCCATGGAG TGCATATTGA AGATCATAGC CTTCGGAGCA 
TTGAACTACT TCAAAGATGC CTGGAACATC TTCGACTGTG TGACTGTACT TGGCAGCATC 
ACTGATATTT TAGTCACAGA GCTGGGGAAC AACTTCATAA ATCTGAGTTT TCTAAGGCTC 
TTCAGAGCTG CTCGTCTCAT TAAGTTGCTC AGGCAGGGTG AAACCATACG CATCCTGCTC 
TGGACTTTTG TCCAGTCTTT CAAGGCTCTG CCGTATGTGT GCCTACTCAT CGCCATGCTG 
TTCTTCATCT ACGCCATCAT TGGCATGCAG CTGTTTGGGA ACATTAAGAT CGAGGAGAAC 
AGTGACAGTG CCATTACCCA GCACAACAAC TTCAGGACTT TTTTCCAGGC CCTAATGCTG 
CTCTTTAGGA GTGCTACAGG TGAGGCATGG CATGACATCA TGCTGTCATG TCTGGGAAAG 
AAGCCGTGTG ATATTCTCTC TGATAACCCG AAGCCAGAAT GCGGGAGCGA GTTTGCCTAC 
CTCTACTTTG TCTCCTTCAT CTTCCTCTGT TCATTTCTGA TGCTGAATCT GTTCGTGGCT 
GTGATCATGG ATAATTTTGA ATACCTGACG CGTGACTCCT CTATCCTGGG ACCCCATCAC 
CTGGACGAGT ATGTGAGGAT CTGGGCAGAA TATGATCCTG CTGCTTGTGG CAGAATCAGT 
TACAGGGATA TGTATGAGAT GCTCAGGCAC ATGTCTCCTC CGTTAGGCCT GGGGAAGAAG 
TGCCCTGCAC GGGTTGCCTA CAAGAGACTG TTGCGGATGG ATCTGCCGGT TGCCGATGAC 
AACACAGTGC ACTTTAATTC TACCCTTATG GCCTTAATCC GAACCGCTCT GGACATCAAG 
ATCGCCAAGG GAGGTGTAGA TAAGCACCAG ATGGATGCTG AATTAAGGAA GGAGATGATG 
GCGATATGGC CTAATCTCTC TCAGAAGAAC CTGGACCTGC TGGTGACTCC TCACAAGTCG 
ACAGACCTGA CAGTAGGAAA GATCTACGCT GCCATGATGA TCATGGAGTA TTATCGGCAA 
AGTAAAGCCA AACGGACACA AGCTCTCCAT GATGAGCAGA ATCGAACGCC ATTAATGTTT 
CAGCGTCTGG AGCCGCCATC TCCTAGCCAG GATGTGGGAC CAGGACTCAC TGGCCTTCCT 
GACACACAAA TGCACCCTGC AAATCATCTG CCTGTTGATG AAAGGATCCC AGAGAGCCAA 
TCATGGGTGA CAGCGCGAGC ACAGGAGATT TCTCAAAAAG CAGGCAACTG GAGTCCAGAG 
GGCCAACATC CAGATGACAC CACAGACAAC AGAAGGCAGT CTCAGACAGT AGAGATGAGA 
GAGATGGGGC GGGACGGGTA CTCGGACACA GAGCAATATC TGCCGATGGA AGGCCATGGC 
AGGGCTGCTT CCATGCCTCG ACTCCCAGCA GACAACCAAC AGCCACGGAG GAAAGGGCGT 
CACCGGGGAG AAAATCTCAC TCAGCCTATC ACAGATAACA GTCCTATGAA GCGCTCGGCT 
TCATCTTTGG GACACGGGCG TGCAGGACGC TCCATGCGAG GTGAGGATTA TGCCATGGAG 
AGGGTTATCC CAGAAGAGGG TCACAGGCAT GGGCATCGCC ACCGAGACCG TAGTCACCGG 
GCGTCAGAAC GCTCCCTGAG CCGATACACT GATGCTGACA CAGGTCTTGG AACTGACCTC 
AGCACAACCA CACAATCAGG AGACCTGCCA TCCAAGGAGA GAGACCGGGG CCGAGCCAAA 
GACCGCAAGC ACCATCATCA TCATCATCAC CACCACGGCT CTTTAGATAA GGAGCACTAC 
GGTCATGAGA GAGACAGAGG AGAGTACGGC CACAGACAGT CCAGAGAGAG AGACCGCCGC 
TGGTCCCGCT CACCCAGTGA GGGCAGAGAG TGTCTGACTC ACAGACAGGG CAGTAGTTCA 
GTAAGCGGCA GTCCAGTCCC GTCCACCTCC GGGACCAGCA CCCCCCGCCG TGGCCGCCGT 
CAGCTCCCCC AAACCCCTGC TACACCCCGG CCACATGTCA CCTACTCACC TGTGGTCCGT 
AAGCCCATCA GCAGTACCCC CCCTCCTGGC CAGCAGAGCC GACTCCCTAC CCCGACCTCC 
CGGCGCTTCA GTCCGACTGG CCCCGAGCCC CCGCTTCCCC CTCATCACCA CCCGTCACCA 
CCCCACCACG GCTCTCCCCG CTCCGGCCGA CATGCACACT GGGGCCCCGA GCCTGCAGAG 
AGCGTGGAGG GTGACGGCTT CTATGACGAC CAAGACTATG AGTTCAATCA CCACGAGCCT 
CCATCCTACG AGCAAAGCCC TGTGCAGGGC AACCCTCACC CCCATTCCCC TAGGACTTCC 
CGCCACAACA CCCCTCCTCA GGGCCAAGCG CACCCACGGC GCATGCCAAA TGGCTACCGT 
TCGTCTTCGC CCTCTCCACA CCACCACGCG CCCGCTCACC CTGGAACTCA TAAACCTCCT 
CATCCCAGAG GGCCCAGAAA GGGCCTCCAT GAACCCTACA GTGAGACTGA CGAGGATGAC 
TGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330087.1
CDS	1509..8597
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRISYRDMYEMLRHMSPPLGLGKKCPARVAYKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQNRTPLMFQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQTVEMREMGRDGYSDTEQYLPMEGHGRAASMPRLPADNQQPRRKGRHRGENLTQPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_021474412.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474412.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACAGGAAGC AGCAAAGGGA AAGACATCAG TACTATCAAG 
TCTCTACGTG TGCTTCGAGT ACTGCGGCCG CTGAAAACCA TCAAACGTCT GCCAAAACTG 
AAGGCTGTCT TTGACTGTGT GGTGAACTCT CTGAAGAATG TGTTGAACAT CCTCATCGTC 
TACATGCTCT TCATGTTCAT CTTCGCTGTG GTGGCTGTGC AGCTCTTTAA GGGACGTTTC 
TTTTACTGCA CTGATGAATC CAAAGAGTTT GAGCGCGACT GCAGGGGCGA GTATCTGGTC 
TATGAAAGAG ATAACGAAGT GAGGTCACAG AAGCGGGAAT GGAAGAAATA CGATTTCCAC 
TACGACAACG TGCTCTGGGC CCTTCTCACC CTCTTCACCG TTTCTACGGG AGAGGGGTGG 
CCGCAAGTGC TAAAACATTC AGTAGATGCG ACCTACGAGA ATCAGGGCCC GAGCCCAGGT 
TACCGGATGG AAATGTCCAT CTTTTACGTG GTGTACTTCG TGGTCTTCCC CTTCTTCTTC 
GTCAACATCT TCGTGGCTCT TATCATCATT ACTTTCCAGG AGCAAGGAGA CAAAATGATG 
GAGGACTATA GCCTGGAAAA GAATGAGAGA GCCTGCATAG ACTTTGCCAT CAACGCTAAG 
CCGCTCACAC GGCACATGCC CCAGAACAAG CAGACCTTTC AGTACCGCAT GTGGGAGTTT 
GTGGTGTCTC CTCCATTTGA ATACACCATC ATGGCCTTGA TCGCGCTCAA CACTATTGTC 
CTTATGATGA AGTATGATGG AGCATCGCTT ACTTATGAAG ATGTGCTAAA ATACCTGAAC 
ATAGTGTTCA CCTCTCTATT CTCCATGGAG TGCATATTGA AGATCATAGC CTTCGGAGCA 
TTGAACTACT TCAAAGATGC CTGGAACATC TTCGACTGTG TGACTGTACT TGGCAGCATC 
ACTGATATTT TAGTCACAGA GCTGGGGAAC AACTTCATAA ATCTGAGTTT TCTAAGGCTC 
TTCAGAGCTG CTCGTCTCAT TAAGTTGCTC AGGCAGGGTG AAACCATACG CATCCTGCTC 
TGGACTTTTG TCCAGTCTTT CAAGGCTCTG CCGTATGTGT GCCTACTCAT CGCCATGCTG 
TTCTTCATCT ACGCCATCAT TGGCATGCAG CTGTTTGGGA ACATTAAGAT CGAGGAGAAC 
AGTGACAGTG CCATTACCCA GCACAACAAC TTCAGGACTT TTTTCCAGGC CCTAATGCTG 
CTCTTTAGGA GTGCTACAGG TGAGGCATGG CATGACATCA TGCTGTCATG TCTGGGAAAG 
AAGCCGTGTG ATATTCTCTC TGATAACCCG AAGCCAGAAT GCGGGAGCGA GTTTGCCTAC 
CTCTACTTTG TCTCCTTCAT CTTCCTCTGT TCATTTCTGA TGCTGAATCT GTTCGTGGCT 
GTGATCATGG ATAATTTTGA ATACCTGACG CGTGACTCCT CTATCCTGGG ACCCCATCAC 
CTGGACGAGT ATGTGAGGAT CTGGGCAGAA TATGATCCTG CTGCTTGTGG CAGAATCAGT 
TACAGGGATA TGTATGAGAT GCTCAGGCAC ATGTCTCCTC CGTTAGGCCT GGGGAAGAAG 
TGCCCTGCAC GGGTTGCCTA CAAGAGACTG TTGCGGATGG ATCTGCCGGT TGCCGATGAC 
AACACAGTGC ACTTTAATTC TACCCTTATG GCCTTAATCC GAACCGCTCT GGACATCAAG 
ATCGCCAAGG GAGGTGTAGA TAAGCACCAG ATGGATGCTG AATTAAGGAA GGAGATGATG 
GCGATATGGC CTAATCTCTC TCAGAAGAAC CTGGACCTGC TGGTGACTCC TCACAAGTCG 
ACAGACCTGA CAGTAGGAAA GATCTACGCT GCCATGATGA TCATGGAGTA TTATCGGCAA 
AGTAAAGCCA AACGGACACA AGCTCTCCAT GATGAGCAGA ATCGAACGCC ATTAATGTTT 
CAGCGTCTGG AGCCGCCATC TCCTAGCCAG GATGTGGGAC CAGGACTCAC TGGCCTTCCT 
GACACACAAA TGCACCCTGC AAATCATCTG CCTGTTGATG AAAGGATCCC AGAGAGCCAA 
TCATGGGTGA CAGCGCGAGC ACAGGAGATT TCTCAAAAAG CAGGCAACTG GAGTCCAGAG 
GGCCAACATC CAGATGACAC CACAGACAAC AGAAGGCAGT CTCAGACAGT AGAGATGAGA 
GAGATGGGGC GGGACGGGTA CTCGGACACA GAGCAATATC TGCCGATGGA AGGCCATGGC 
AGGGCTGCTT CCATGCCTCG ACTCCCAGCA GACAACCAAC AGCCACGGAG GAAAGGGCGT 
CACCGGGGAG AAAATCTCAC TCAGCCTATC ACAGATAACA GTCCTATGAA GCGCTCGGCT 
TCATCTTTGG GACACGGGCG TGCAGGACGC TCCATGCGAG GTGAGGATTA TGCCATGGAG 
AGGGTTATCC CAGAAGAGGG TCACAGGCAT GGGCATCGCC ACCGAGACCG TAGTCACCGG 
GCGTCAGAAC GCTCCCTGAG CCGATACACT GATGCTGACA CAGGTCTTGG AACTGACCTC 
AGCACAACCA CACAATCAGG AGACCTGCCA TCCAAGGAGA GAGACCGGGG CCGAGCCAAA 
GACCGCAAGC ACCATCATCA TCATCATCAC CACCACGGCT CTTTAGATAA GGAGCACTAC 
GGTCATGAGA GAGACAGAGG AGAGTACGGC CACAGACAGT CCAGAGAGAG AGACCGCCGC 
TGGTCCCGCT CACCCAGTGA GGGCAGAGAG TGTCTGACTC ACAGACAGGG CAGTAGTTCA 
GTAAGCGGCA GTCCAGTCCC GTCCACCTCC GGGACCAGCA CCCCCCGCCG TGGCCGCCGT 
CAGCTCCCCC AAACCCCTGC TACACCCCGG CCACATGTCA CCTACTCACC TGTGGTCCGT 
AAGCCCATCA GCAGTACCCC CCCTCCTGGC CAGCAGAGCC GACTCCCTAC CCCGACCTCC 
CGGCGCTTCA GTCCGACTGG CCCCGAGCCC CCGCTTCCCC CTCATCACCA CCCGTCACCA 
CCCCACCACG GCTCTCCCCG CTCCGGCCGA CATGCACACT GGGGCCCCGA GCCTGCAGAG 
AGCGTGGAGG GTGACGGCTT CTATGACGAC CAAGACTATG AGTTCAATCA CCACGAGCCT 
CCATCCTACG AGCAAAGCCC TGTGCAGGGC AACCCTCACC CCCATTCCCC TAGGACTTCC 
CGCCACAACA CCCCTCCTCA GGGCCAAGCG CACCCACGGC GCATGCCAAA TGGCTACCGT 
TCGTCTTCGC CCTCTCCACA CCACCACGCG CCCGCTCACC CTGGAACTCA TAAACCTCCT 
CATCCCAGAG GGCCCAGAAA GGGCCTCCAT GAACCCTACA GTGAGACTGA CGAGGATGAC 
TGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_021474420.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330095.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X16, mRNA.

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CloneID	ODa141796
Clone ID Related Accession (Same CDS sequence)	XM_021474420.1
Accession Version	XM_021474420.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCTAAGGA GCAGCAGAAG AACCACAAAG GTTGTAAGTC GGTATGGGAG 
CAGCGCACTA GTGAGTTAAG ACGACAGACC CTCGTGAACA GCAGAGAGGC TCTTTATAAT 
GAGTTGGACC CTGAAGACCG TTGGAAGGTC TCATACTCCC GTCACATACG TCCCGACATG 
AAAACTCACC TGGACCGGCC GTTAGTCGTG GACCCTCAGG AGAACCGCAA TAACAACACT 
AACAAAACCC GTCCGGGTGA CGGCCAACCT CAGCATTCCC ATCAGCTCCT ACACAAACAG 
CCCAACTTCA ATGAGGCCGA GACTGGAGGA GGTGGAGGGT CTGAGTCCTG GCCCGCACTG 
GAAAGAGGAG ACTCAACAGG GTCCCGCCGC AGTCTTTTGG GCTCTGAGCA CTATGAGGAG 
GGTAGAGAGT CCCACCGCAG ACACCAGCAC AGCCAGCACT GCAGTCAGGC CCGTCAACAT 
CGCAAAGCTG CAGAGGGCGG TGGAGAGCCT GGAGGGAGGA GACACAGGAG TAAGGCCAAG 
GAAAGAGGAC CTGAGTGTGA GCATTCAGAT GGAGGGGAGC GGAAACACAG ACGGCATCGG 
CATGAGGGTG AGGGGAGGAA AGAGAGAGGG GTACGACACC GAAACAGGAG GGATGGCCAT 
GCAAGTGGCC CGACTCTCTC TACAACCCGT CCCATTCAGA AGACTCTGTC CAGGCAGGAC 
AGTCAGTACA GCGAAGACCT GGACAATGCC ATGAACAACA AACTTGCTAC CCAGCCACAT 
GATAGTCTGC TTAATCTGGC CAACACTGCC CACTCTGCCG GCCTGGCACA TACTGGTACA 
GAGAGCAGCC TCATCCTCAC CAACCCTTCC TCTACCATTG CAAATGTTAC TAGCATCGGT 
CACCTGGGTA TGAAACCTGA GTACACTGCC GTGGACATTC CTCCCATGTT CCCCTCCAGT 
AATGCCATTC TACAGGTCAA TAAGAATGCC AACACGGAGC CTCTGCCAAA GAAGGAAGAC 
ACAAAGGGTG ACGATGATGA TGATGAGAAA GATGACGGAG GGCCTAAACC CATGCCACCC 
TACACCTCAA TGTTCATTCT GACCACCACC AACCCATTTC GGAGATTGTG TCACTATATC 
GTCACACTGA GATATTTTGA GATGTGTATT CTACTGGTTA TTGCTATGAG CAGTATTGCC 
CTGGCTGCTG AAGACCCTGT CTGGCCTGAA TCTCCCCGCA ACAATGTCCT ACGATACTTT 
GACTATGTGT TCACTGGTGT GTTCACCTTT GAGATGCTCA TTAAGATGGT AGATTTAGGT 
CTTGTCCTGC ATCAAGGCTC ATACTTTCGT GATCTGTGGA ACATCCTGGA CTTTATAGTG 
GTTAGTGGTG CCCTGGTGGC GTTCGCCTTC ACCGGAGGCA GCGGAGGAAG CAGCAAAGGG 
AAAGACATCA GTACTATCAA GTCTCTACGT GTGCTTCGAG TACTGCGGCC GCTGAAAACC 
ATCAAACGTC TGCCAAAACT GAAGGCTGTC TTTGACTGTG TGGTGAACTC TCTGAAGAAT 
GTGTTGAACA TCCTCATCGT CTACATGCTC TTCATGTTCA TCTTCGCTGT GGTGGCTGTG 
CAGCTCTTTA AGGGACGTTT CTTTTACTGC ACTGATGAAT CCAAAGAGTT TGAGCGCGAC 
TGCAGGGGCG AGTATCTGGT CTATGAAAGA GATAACGAAG TGAGGTCACA GAAGCGGGAA 
TGGAAGAAAT ACGATTTCCA CTACGACAAC GTGCTCTGGG CCCTTCTCAC CCTCTTCACC 
GTTTCTACGG GAGAGGGGTG GCCGCAAGTG CTAAAACATT CAGTAGATGC GACCTACGAG 
AATCAGGGCC CGAGCCCAGG TTACCGGATG GAAATGTCCA TCTTTTACGT GGTGTACTTC 
GTGGTCTTCC CCTTCTTCTT CGTCAACATC TTCGTGGCTC TTATCATCAT TACTTTCCAG 
GAGCAAGGAG ACAAAATGAT GGAGGACTAT AGCCTGGAAA AGAATGAGAG AGCCTGCATA 
GACTTTGCCA TCAACGCTAA GCCGCTCACA CGGCACATGC CCCAGAACAA GCAGACCTTT 
CAGTACCGCA TGTGGGAGTT TGTGGTGTCT CCTCCATTTG AATACACCAT CATGGCCTTG 
ATCGCGCTCA ACACTATTGT CCTTATGATG AAGTATGATG GAGCATCGCT TACTTATGAA 
GATGTGCTAA AATACCTGAA CATAGTGTTC ACCTCTCTAT TCTCCATGGA GTGCATATTG 
AAGATCATAG CCTTCGGAGC ATTGAACTAC TTCAAAGATG CCTGGAACAT CTTCGACTGT 
GTGACTGTAC TTGGCAGCAT CACTGATATT TTAGTCACAG AGCTGGGGAT GAACAACTTC 
ATAAATCTGA GTTTTCTAAG GCTCTTCAGA GCTGCTCGTC TCATTAAGTT GCTCAGGCAG 
GGTGAAACCA TACGCATCCT GCTCTGGACT TTTGTCCAGT CTTTCAAGGC TCTGCCGTAT 
GTGTGCCTAC TCATCGCCAT GCTGTTCTTC ATCTACGCCA TCATTGGCAT GCAGCTGTTT 
GGGAACATTA AGATCGAGGA GAACAGTGAC AGTGCCATTA CCCAGCACAA CAACTTCAGG 
ACTTTTTTCC AGGCCCTAAT GCTGCTCTTT AGGAGTGCTA CAGGTGAGGC ATGGCATGAC 
ATCATGCTGT CATGTCTGGG AAAGAAGCCG TGTGATATTC TCTCTGATAA CCCGAAGCCA 
GAATGCGGGA GCGAGTTTGC CTACCTCTAC TTTGTCTCCT TCATCTTCCT CTGTTCATTT 
CTGATGCTGA ATCTGTTCGT GGCTGTGATC ATGGATAATT TTGAATACCT GACGCGTGAC 
TCCTCTATCC TGGGACCCCA TCACCTGGAC GAGTATGTGA GGATCTGGGC AGAATATGAT 
CCTGCTGCTT GCGGGCGCAT TCATTATAAG GATATGTACA GTTTATTGCG AGTTATCGAC 
CCCCCTCTGG GCTTAGGCAA GAAATGTCCT CATAGGGTGG CCTGCAAGAG ACTGTTGCGG 
ATGGATCTGC CGGTTGCCGA TGACAACACA GTGCACTTTA ATTCTACCCT TATGGCCTTA 
ATCCGAACCG CTCTGGACAT CAAGATCGCC AAGGGTGGGG AAGGAGGTGT AGATAAGCAC 
CAGATGGATG CTGAATTAAG GAAGGAGATG ATGGCGATAT GGCCTAATCT CTCTCAGAAG 
AACCTGGACC TGCTGGTGAC TCCTCACAAG TCAGCGACAG ACCTGACAGT AGGAAAGATC 
TACGCTGCCA TGATGATCAT GGAGTATTAT CGGCAAAGTA AAGCCAAACG GACACAAGCT 
CTCCATGATG AGCAGAATCG AACGCCATTA ATGTTTCAGC GTCTGGAGCC GCCATCTCCT 
AGCCAGGATG TGGGACCAGG ACTCACTGGC CTTCCTGACA CACAAATGCA CCCTGCAAAT 
CATCTGCCTG TTGATGAAAG GATCCCAGAG AGCCAATCAT GGGTGACAGC GCGAGCACAG 
GAGATTTCTC AAAAAGCAGG CAACTGGAGT CCAGAGGGCC AACATCCAGA TGACACCACA 
GACAACAGAA GGCAGTCTCA GACAGTAGAG ATGAGAGAGA TGGGGCGGGA CGGGTACTCG 
GACACAGAGC AATATCTGCC GATGGAAGGC CATGGCAGGG CTGCTTCCAT GCCTCGACTC 
CCAGCAGACA ACCAACAGCC ACGGAGGAAA GGGCGTCACC GGGGAGAAAA TCTCACTCAG 
CCTATCACAG ATAACAGTCC TATGAAGCGC TCGGCTTCAT CTTTGGGACA CGGGCGTGCA 
GGACGCTCCA TGCGAGGTGA GGATTATGCC ATGGAGAGGG TTATCCCAGA AGAGGGTCAC 
AGGCATGGGC ATCGCCACCG AGACCGTAGT CACCGGGCGT CAGAACGCTC CCTGAGCCGA 
TACACTGATG CTGACACAGG TCTTGGAACT GACCTCAGCA CAACCACACA ATCAGGAGAC 
CTGCCATCCA AGGAGAGAGA CCGGGGCCGA GCCAAAGACC GCAAGCACCA TCATCATCAT 
CATCACCACC ACGGCTCTTT AGATAAGGAG CACTACGGTC ATGAGAGAGA CAGAGGAGAG 
TACGGCCACA GACAGTCCAG AGAGAGAGAC CGCCGCTGGT CCCGCTCACC CAGTGAGGGC 
AGAGAGTGTC TGACTCACAG ACAGGGCAGT AGTTCAGTAA GCGGCAGTCC AGTCCCGTCC 
ACCTCCGGGA CCAGCACCCC CCGCCGTGGC CGCCGTCAGC TCCCCCAAAC CCCTGCTACA 
CCCCGGCCAC ATGTCACCTA CTCACCTGTG GTCCGTAAGC CCATCAGCAG TACCCCCCCT 
CCTGGCCAGC AGAGCCGACT CCCTACCCCG ACCTCCCGGC GCTTCAGTCC GACTGGCCCC 
GAGCCCCCGC TTCCCCCTCA TCACCACCCG TCACCACCCC ACCACGGCTC TCCCCGCTCC 
GGCCGACATG CACACTGGGG CCCCGAGCCT GCAGAGAGCG TGGAGGGTGA CGGCTTCTAT 
GACGACCAAG ACTATGAGTT CAATCACCAC GAGCCTCCAT CCTACGAGCA AAGCCCTGTG 
CAGGGCAACC CTCACCCCCA TTCCCCTAGG ACTTCCCGCC ACAACACCCC TCCTCAGGGC 
CAAGCGCACC CACGGCGCAT GCCAAATGGC TACCGTTCGT CTTCGCCCTC TCCACACCAC 
CACGCGCCCG CTCACCCTGG AACTCATAAA CCTCCTCATC CCAGAGGGCC CAGAAAGGGC 
CTCCATGAAC CCTACAGTGA GACTGACGAG GATGACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330095.1
CDS	1506..8570
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGGSGGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGMNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGEGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQNRTPLMFQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQTVEMREMGRDGYSDTEQYLPMEGHGRAASMPRLPADNQQPRRKGRHRGENLTQPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_021474420.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474420.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCTAAGGA GCAGCAGAAG AACCACAAAG GTTGTAAGTC GGTATGGGAG 
CAGCGCACTA GTGAGTTAAG ACGACAGACC CTCGTGAACA GCAGAGAGGC TCTTTATAAT 
GAGTTGGACC CTGAAGACCG TTGGAAGGTC TCATACTCCC GTCACATACG TCCCGACATG 
AAAACTCACC TGGACCGGCC GTTAGTCGTG GACCCTCAGG AGAACCGCAA TAACAACACT 
AACAAAACCC GTCCGGGTGA CGGCCAACCT CAGCATTCCC ATCAGCTCCT ACACAAACAG 
CCCAACTTCA ATGAGGCCGA GACTGGAGGA GGTGGAGGGT CTGAGTCCTG GCCCGCACTG 
GAAAGAGGAG ACTCAACAGG GTCCCGCCGC AGTCTTTTGG GCTCTGAGCA CTATGAGGAG 
GGTAGAGAGT CCCACCGCAG ACACCAGCAC AGCCAGCACT GCAGTCAGGC CCGTCAACAT 
CGCAAAGCTG CAGAGGGCGG TGGAGAGCCT GGAGGGAGGA GACACAGGAG TAAGGCCAAG 
GAAAGAGGAC CTGAGTGTGA GCATTCAGAT GGAGGGGAGC GGAAACACAG ACGGCATCGG 
CATGAGGGTG AGGGGAGGAA AGAGAGAGGG GTACGACACC GAAACAGGAG GGATGGCCAT 
GCAAGTGGCC CGACTCTCTC TACAACCCGT CCCATTCAGA AGACTCTGTC CAGGCAGGAC 
AGTCAGTACA GCGAAGACCT GGACAATGCC ATGAACAACA AACTTGCTAC CCAGCCACAT 
GATAGTCTGC TTAATCTGGC CAACACTGCC CACTCTGCCG GCCTGGCACA TACTGGTACA 
GAGAGCAGCC TCATCCTCAC CAACCCTTCC TCTACCATTG CAAATGTTAC TAGCATCGGT 
CACCTGGGTA TGAAACCTGA GTACACTGCC GTGGACATTC CTCCCATGTT CCCCTCCAGT 
AATGCCATTC TACAGGTCAA TAAGAATGCC AACACGGAGC CTCTGCCAAA GAAGGAAGAC 
ACAAAGGGTG ACGATGATGA TGATGAGAAA GATGACGGAG GGCCTAAACC CATGCCACCC 
TACACCTCAA TGTTCATTCT GACCACCACC AACCCATTTC GGAGATTGTG TCACTATATC 
GTCACACTGA GATATTTTGA GATGTGTATT CTACTGGTTA TTGCTATGAG CAGTATTGCC 
CTGGCTGCTG AAGACCCTGT CTGGCCTGAA TCTCCCCGCA ACAATGTCCT ACGATACTTT 
GACTATGTGT TCACTGGTGT GTTCACCTTT GAGATGCTCA TTAAGATGGT AGATTTAGGT 
CTTGTCCTGC ATCAAGGCTC ATACTTTCGT GATCTGTGGA ACATCCTGGA CTTTATAGTG 
GTTAGTGGTG CCCTGGTGGC GTTCGCCTTC ACCGGAGGCA GCGGAGGAAG CAGCAAAGGG 
AAAGACATCA GTACTATCAA GTCTCTACGT GTGCTTCGAG TACTGCGGCC GCTGAAAACC 
ATCAAACGTC TGCCAAAACT GAAGGCTGTC TTTGACTGTG TGGTGAACTC TCTGAAGAAT 
GTGTTGAACA TCCTCATCGT CTACATGCTC TTCATGTTCA TCTTCGCTGT GGTGGCTGTG 
CAGCTCTTTA AGGGACGTTT CTTTTACTGC ACTGATGAAT CCAAAGAGTT TGAGCGCGAC 
TGCAGGGGCG AGTATCTGGT CTATGAAAGA GATAACGAAG TGAGGTCACA GAAGCGGGAA 
TGGAAGAAAT ACGATTTCCA CTACGACAAC GTGCTCTGGG CCCTTCTCAC CCTCTTCACC 
GTTTCTACGG GAGAGGGGTG GCCGCAAGTG CTAAAACATT CAGTAGATGC GACCTACGAG 
AATCAGGGCC CGAGCCCAGG TTACCGGATG GAAATGTCCA TCTTTTACGT GGTGTACTTC 
GTGGTCTTCC CCTTCTTCTT CGTCAACATC TTCGTGGCTC TTATCATCAT TACTTTCCAG 
GAGCAAGGAG ACAAAATGAT GGAGGACTAT AGCCTGGAAA AGAATGAGAG AGCCTGCATA 
GACTTTGCCA TCAACGCTAA GCCGCTCACA CGGCACATGC CCCAGAACAA GCAGACCTTT 
CAGTACCGCA TGTGGGAGTT TGTGGTGTCT CCTCCATTTG AATACACCAT CATGGCCTTG 
ATCGCGCTCA ACACTATTGT CCTTATGATG AAGTATGATG GAGCATCGCT TACTTATGAA 
GATGTGCTAA AATACCTGAA CATAGTGTTC ACCTCTCTAT TCTCCATGGA GTGCATATTG 
AAGATCATAG CCTTCGGAGC ATTGAACTAC TTCAAAGATG CCTGGAACAT CTTCGACTGT 
GTGACTGTAC TTGGCAGCAT CACTGATATT TTAGTCACAG AGCTGGGGAT GAACAACTTC 
ATAAATCTGA GTTTTCTAAG GCTCTTCAGA GCTGCTCGTC TCATTAAGTT GCTCAGGCAG 
GGTGAAACCA TACGCATCCT GCTCTGGACT TTTGTCCAGT CTTTCAAGGC TCTGCCGTAT 
GTGTGCCTAC TCATCGCCAT GCTGTTCTTC ATCTACGCCA TCATTGGCAT GCAGCTGTTT 
GGGAACATTA AGATCGAGGA GAACAGTGAC AGTGCCATTA CCCAGCACAA CAACTTCAGG 
ACTTTTTTCC AGGCCCTAAT GCTGCTCTTT AGGAGTGCTA CAGGTGAGGC ATGGCATGAC 
ATCATGCTGT CATGTCTGGG AAAGAAGCCG TGTGATATTC TCTCTGATAA CCCGAAGCCA 
GAATGCGGGA GCGAGTTTGC CTACCTCTAC TTTGTCTCCT TCATCTTCCT CTGTTCATTT 
CTGATGCTGA ATCTGTTCGT GGCTGTGATC ATGGATAATT TTGAATACCT GACGCGTGAC 
TCCTCTATCC TGGGACCCCA TCACCTGGAC GAGTATGTGA GGATCTGGGC AGAATATGAT 
CCTGCTGCTT GCGGGCGCAT TCATTATAAG GATATGTACA GTTTATTGCG AGTTATCGAC 
CCCCCTCTGG GCTTAGGCAA GAAATGTCCT CATAGGGTGG CCTGCAAGAG ACTGTTGCGG 
ATGGATCTGC CGGTTGCCGA TGACAACACA GTGCACTTTA ATTCTACCCT TATGGCCTTA 
ATCCGAACCG CTCTGGACAT CAAGATCGCC AAGGGTGGGG AAGGAGGTGT AGATAAGCAC 
CAGATGGATG CTGAATTAAG GAAGGAGATG ATGGCGATAT GGCCTAATCT CTCTCAGAAG 
AACCTGGACC TGCTGGTGAC TCCTCACAAG TCAGCGACAG ACCTGACAGT AGGAAAGATC 
TACGCTGCCA TGATGATCAT GGAGTATTAT CGGCAAAGTA AAGCCAAACG GACACAAGCT 
CTCCATGATG AGCAGAATCG AACGCCATTA ATGTTTCAGC GTCTGGAGCC GCCATCTCCT 
AGCCAGGATG TGGGACCAGG ACTCACTGGC CTTCCTGACA CACAAATGCA CCCTGCAAAT 
CATCTGCCTG TTGATGAAAG GATCCCAGAG AGCCAATCAT GGGTGACAGC GCGAGCACAG 
GAGATTTCTC AAAAAGCAGG CAACTGGAGT CCAGAGGGCC AACATCCAGA TGACACCACA 
GACAACAGAA GGCAGTCTCA GACAGTAGAG ATGAGAGAGA TGGGGCGGGA CGGGTACTCG 
GACACAGAGC AATATCTGCC GATGGAAGGC CATGGCAGGG CTGCTTCCAT GCCTCGACTC 
CCAGCAGACA ACCAACAGCC ACGGAGGAAA GGGCGTCACC GGGGAGAAAA TCTCACTCAG 
CCTATCACAG ATAACAGTCC TATGAAGCGC TCGGCTTCAT CTTTGGGACA CGGGCGTGCA 
GGACGCTCCA TGCGAGGTGA GGATTATGCC ATGGAGAGGG TTATCCCAGA AGAGGGTCAC 
AGGCATGGGC ATCGCCACCG AGACCGTAGT CACCGGGCGT CAGAACGCTC CCTGAGCCGA 
TACACTGATG CTGACACAGG TCTTGGAACT GACCTCAGCA CAACCACACA ATCAGGAGAC 
CTGCCATCCA AGGAGAGAGA CCGGGGCCGA GCCAAAGACC GCAAGCACCA TCATCATCAT 
CATCACCACC ACGGCTCTTT AGATAAGGAG CACTACGGTC ATGAGAGAGA CAGAGGAGAG 
TACGGCCACA GACAGTCCAG AGAGAGAGAC CGCCGCTGGT CCCGCTCACC CAGTGAGGGC 
AGAGAGTGTC TGACTCACAG ACAGGGCAGT AGTTCAGTAA GCGGCAGTCC AGTCCCGTCC 
ACCTCCGGGA CCAGCACCCC CCGCCGTGGC CGCCGTCAGC TCCCCCAAAC CCCTGCTACA 
CCCCGGCCAC ATGTCACCTA CTCACCTGTG GTCCGTAAGC CCATCAGCAG TACCCCCCCT 
CCTGGCCAGC AGAGCCGACT CCCTACCCCG ACCTCCCGGC GCTTCAGTCC GACTGGCCCC 
GAGCCCCCGC TTCCCCCTCA TCACCACCCG TCACCACCCC ACCACGGCTC TCCCCGCTCC 
GGCCGACATG CACACTGGGG CCCCGAGCCT GCAGAGAGCG TGGAGGGTGA CGGCTTCTAT 
GACGACCAAG ACTATGAGTT CAATCACCAC GAGCCTCCAT CCTACGAGCA AAGCCCTGTG 
CAGGGCAACC CTCACCCCCA TTCCCCTAGG ACTTCCCGCC ACAACACCCC TCCTCAGGGC 
CAAGCGCACC CACGGCGCAT GCCAAATGGC TACCGTTCGT CTTCGCCCTC TCCACACCAC 
CACGCGCCCG CTCACCCTGG AACTCATAAA CCTCCTCATC CCAGAGGGCC CAGAAAGGGC 
CTCCATGAAC CCTACAGTGA GACTGACGAG GATGACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_021474422.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330097.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X17, mRNA.

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CloneID	ODa141797
Clone ID Related Accession (Same CDS sequence)	XM_021474422.1
Accession Version	XM_021474422.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG 
GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT 
GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC 
CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC 
ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT 
GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT 
GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT 
GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT 
CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA 
AGGCAGTCTC AGCAGCCACG GAGGAAAGGG CGTCACCGGG GAGAAAATCT CACTCAGCCT 
ATCACAGATA ACAGTCCTAT GAAGCGCTCG GCTTCATCTT TGGGACACGG GCGTGCAGGA 
CGCTCCATGC GAGGTGAGGA TTATGCCATG GAGAGGGTTA TCCCAGAAGA GGGTCACAGG 
CATGGGCATC GCCACCGAGA CCGTAGTCAC CGGGCGTCAG AACGCTCCCT GAGCCGATAC 
ACTGATGCTG ACACAGGTCT TGGAACTGAC CTCAGCACAA CCACACAATC AGGAGACCTG 
CCATCCAAGG AGAGAGACCG GGGCCGAGCC AAAGACCGCA AGCACCATCA TCATCATCAT 
CACCACCACG GCTCTTTAGA TAAGGAGCAC TACGGTCATG AGAGAGACAG AGGAGAGTAC 
GGCCACAGAC AGTCCAGAGA GAGAGACCGC CGCTGGTCCC GCTCACCCAG TGAGGGCAGA 
GAGTGTCTGA CTCACAGACA GGGCAGTAGT TCAGTAAGCG GCAGTCCAGT CCCGTCCACC 
TCCGGGACCA GCACCCCCCG CCGTGGCCGC CGTCAGCTCC CCCAAACCCC TGCTACACCC 
CGGCCACATG TCACCTACTC ACCTGTGGTC CGTAAGCCCA TCAGCAGTAC CCCCCCTCCT 
GGCCAGCAGA GCCGACTCCC TACCCCGACC TCCCGGCGCT TCAGTCCGAC TGGCCCCGAG 
CCCCCGCTTC CCCCTCATCA CCACCCGTCA CCACCCCACC ACGGCTCTCC CCGCTCCGGC 
CGACATGCAC ACTGGGGCCC CGAGCCTGCA GAGAGCGTGG AGGGTGACGG CTTCTATGAC 
GACCAAGACT ATGAGTTCAA TCACCACGAG CCTCCATCCT ACGAGCAAAG CCCTGTGCAG 
GGCAACCCTC ACCCCCATTC CCCTAGGACT TCCCGCCACA ACACCCCTCC TCAGGGCCAA 
GCGCACCCAC GGCGCATGCC AAATGGCTAC CGTTCGTCTT CGCCCTCTCC ACACCACCAC 
GCGCCCGCTC ACCCTGGAAC TCATAAACCT CCTCATCCCA GAGGGCCCAG AAAGGGCCTC 
CATGAACCCT ACAGTGAGAC TGACGAGGAT GACTGGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330097.1
CDS	1509..8510
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGGSGGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGMNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGEGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQNRTPLMFQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQQPRRKGRHRGENLTQPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_021474422.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474422.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG 
GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT 
GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC 
CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC 
ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT 
GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT 
GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT 
GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT 
CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA 
AGGCAGTCTC AGCAGCCACG GAGGAAAGGG CGTCACCGGG GAGAAAATCT CACTCAGCCT 
ATCACAGATA ACAGTCCTAT GAAGCGCTCG GCTTCATCTT TGGGACACGG GCGTGCAGGA 
CGCTCCATGC GAGGTGAGGA TTATGCCATG GAGAGGGTTA TCCCAGAAGA GGGTCACAGG 
CATGGGCATC GCCACCGAGA CCGTAGTCAC CGGGCGTCAG AACGCTCCCT GAGCCGATAC 
ACTGATGCTG ACACAGGTCT TGGAACTGAC CTCAGCACAA CCACACAATC AGGAGACCTG 
CCATCCAAGG AGAGAGACCG GGGCCGAGCC AAAGACCGCA AGCACCATCA TCATCATCAT 
CACCACCACG GCTCTTTAGA TAAGGAGCAC TACGGTCATG AGAGAGACAG AGGAGAGTAC 
GGCCACAGAC AGTCCAGAGA GAGAGACCGC CGCTGGTCCC GCTCACCCAG TGAGGGCAGA 
GAGTGTCTGA CTCACAGACA GGGCAGTAGT TCAGTAAGCG GCAGTCCAGT CCCGTCCACC 
TCCGGGACCA GCACCCCCCG CCGTGGCCGC CGTCAGCTCC CCCAAACCCC TGCTACACCC 
CGGCCACATG TCACCTACTC ACCTGTGGTC CGTAAGCCCA TCAGCAGTAC CCCCCCTCCT 
GGCCAGCAGA GCCGACTCCC TACCCCGACC TCCCGGCGCT TCAGTCCGAC TGGCCCCGAG 
CCCCCGCTTC CCCCTCATCA CCACCCGTCA CCACCCCACC ACGGCTCTCC CCGCTCCGGC 
CGACATGCAC ACTGGGGCCC CGAGCCTGCA GAGAGCGTGG AGGGTGACGG CTTCTATGAC 
GACCAAGACT ATGAGTTCAA TCACCACGAG CCTCCATCCT ACGAGCAAAG CCCTGTGCAG 
GGCAACCCTC ACCCCCATTC CCCTAGGACT TCCCGCCACA ACACCCCTCC TCAGGGCCAA 
GCGCACCCAC GGCGCATGCC AAATGGCTAC CGTTCGTCTT CGCCCTCTCC ACACCACCAC 
GCGCCCGCTC ACCCTGGAAC TCATAAACCT CCTCATCCCA GAGGGCCCAG AAAGGGCCTC 
CATGAACCCT ACAGTGAGAC TGACGAGGAT GACTGGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_021474423.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330098.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X18, mRNA.

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CloneID	ODa141798
Clone ID Related Accession (Same CDS sequence)	XM_021474423.1
Accession Version	XM_021474423.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6999bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG 
GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT 
GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC 
CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC 
ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT 
GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT 
GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT 
GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT 
CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA 
AGGCAGTCTC AGCAGCCACG GAGGAAAGGG CGTCACCGGG GAGAAAATCT CACTCCTATC 
ACAGATAACA GTCCTATGAA GCGCTCGGCT TCATCTTTGG GACACGGGCG TGCAGGACGC 
TCCATGCGAG GTGAGGATTA TGCCATGGAG AGGGTTATCC CAGAAGAGGG TCACAGGCAT 
GGGCATCGCC ACCGAGACCG TAGTCACCGG GCGTCAGAAC GCTCCCTGAG CCGATACACT 
GATGCTGACA CAGGTCTTGG AACTGACCTC AGCACAACCA CACAATCAGG AGACCTGCCA 
TCCAAGGAGA GAGACCGGGG CCGAGCCAAA GACCGCAAGC ACCATCATCA TCATCATCAC 
CACCACGGCT CTTTAGATAA GGAGCACTAC GGTCATGAGA GAGACAGAGG AGAGTACGGC 
CACAGACAGT CCAGAGAGAG AGACCGCCGC TGGTCCCGCT CACCCAGTGA GGGCAGAGAG 
TGTCTGACTC ACAGACAGGG CAGTAGTTCA GTAAGCGGCA GTCCAGTCCC GTCCACCTCC 
GGGACCAGCA CCCCCCGCCG TGGCCGCCGT CAGCTCCCCC AAACCCCTGC TACACCCCGG 
CCACATGTCA CCTACTCACC TGTGGTCCGT AAGCCCATCA GCAGTACCCC CCCTCCTGGC 
CAGCAGAGCC GACTCCCTAC CCCGACCTCC CGGCGCTTCA GTCCGACTGG CCCCGAGCCC 
CCGCTTCCCC CTCATCACCA CCCGTCACCA CCCCACCACG GCTCTCCCCG CTCCGGCCGA 
CATGCACACT GGGGCCCCGA GCCTGCAGAG AGCGTGGAGG GTGACGGCTT CTATGACGAC 
CAAGACTATG AGTTCAATCA CCACGAGCCT CCATCCTACG AGCAAAGCCC TGTGCAGGGC 
AACCCTCACC CCCATTCCCC TAGGACTTCC CGCCACAACA CCCCTCCTCA GGGCCAAGCG 
CACCCACGGC GCATGCCAAA TGGCTACCGT TCGTCTTCGC CCTCTCCACA CCACCACGCG 
CCCGCTCACC CTGGAACTCA TAAACCTCCT CATCCCAGAG GGCCCAGAAA GGGCCTCCAT 
GAACCCTACA GTGAGACTGA CGAGGATGAC TGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330098.1
CDS	1509..8507
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGGSGGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGMNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGEGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQNRTPLMFQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQQPRRKGRHRGENLTPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_021474423.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474423.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG 
GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT 
GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC 
CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC 
ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT 
GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT 
GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT 
GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT 
CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA 
AGGCAGTCTC AGCAGCCACG GAGGAAAGGG CGTCACCGGG GAGAAAATCT CACTCCTATC 
ACAGATAACA GTCCTATGAA GCGCTCGGCT TCATCTTTGG GACACGGGCG TGCAGGACGC 
TCCATGCGAG GTGAGGATTA TGCCATGGAG AGGGTTATCC CAGAAGAGGG TCACAGGCAT 
GGGCATCGCC ACCGAGACCG TAGTCACCGG GCGTCAGAAC GCTCCCTGAG CCGATACACT 
GATGCTGACA CAGGTCTTGG AACTGACCTC AGCACAACCA CACAATCAGG AGACCTGCCA 
TCCAAGGAGA GAGACCGGGG CCGAGCCAAA GACCGCAAGC ACCATCATCA TCATCATCAC 
CACCACGGCT CTTTAGATAA GGAGCACTAC GGTCATGAGA GAGACAGAGG AGAGTACGGC 
CACAGACAGT CCAGAGAGAG AGACCGCCGC TGGTCCCGCT CACCCAGTGA GGGCAGAGAG 
TGTCTGACTC ACAGACAGGG CAGTAGTTCA GTAAGCGGCA GTCCAGTCCC GTCCACCTCC 
GGGACCAGCA CCCCCCGCCG TGGCCGCCGT CAGCTCCCCC AAACCCCTGC TACACCCCGG 
CCACATGTCA CCTACTCACC TGTGGTCCGT AAGCCCATCA GCAGTACCCC CCCTCCTGGC 
CAGCAGAGCC GACTCCCTAC CCCGACCTCC CGGCGCTTCA GTCCGACTGG CCCCGAGCCC 
CCGCTTCCCC CTCATCACCA CCCGTCACCA CCCCACCACG GCTCTCCCCG CTCCGGCCGA 
CATGCACACT GGGGCCCCGA GCCTGCAGAG AGCGTGGAGG GTGACGGCTT CTATGACGAC 
CAAGACTATG AGTTCAATCA CCACGAGCCT CCATCCTACG AGCAAAGCCC TGTGCAGGGC 
AACCCTCACC CCCATTCCCC TAGGACTTCC CGCCACAACA CCCCTCCTCA GGGCCAAGCG 
CACCCACGGC GCATGCCAAA TGGCTACCGT TCGTCTTCGC CCTCTCCACA CCACCACGCG 
CCCGCTCACC CTGGAACTCA TAAACCTCCT CATCCCAGAG GGCCCAGAAA GGGCCTCCAT 
GAACCCTACA GTGAGACTGA CGAGGATGAC TGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_021474428.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330103.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X19, mRNA.

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CloneID	ODa141799
Clone ID Related Accession (Same CDS sequence)	XM_021474428.1
Accession Version	XM_021474428.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6960bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG 
GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT 
GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC 
CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC 
ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT 
GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT 
GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT 
GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT 
CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA 
AGGCAGTCTC AGCAGCCTAT CACAGATAAC AGTCCTATGA AGCGCTCGGC TTCATCTTTG 
GGACACGGGC GTGCAGGACG CTCCATGCGA GGTGAGGATT ATGCCATGGA GAGGGTTATC 
CCAGAAGAGG GTCACAGGCA TGGGCATCGC CACCGAGACC GTAGTCACCG GGCGTCAGAA 
CGCTCCCTGA GCCGATACAC TGATGCTGAC ACAGGTCTTG GAACTGACCT CAGCACAACC 
ACACAATCAG GAGACCTGCC ATCCAAGGAG AGAGACCGGG GCCGAGCCAA AGACCGCAAG 
CACCATCATC ATCATCATCA CCACCACGGC TCTTTAGATA AGGAGCACTA CGGTCATGAG 
AGAGACAGAG GAGAGTACGG CCACAGACAG TCCAGAGAGA GAGACCGCCG CTGGTCCCGC 
TCACCCAGTG AGGGCAGAGA GTGTCTGACT CACAGACAGG GCAGTAGTTC AGTAAGCGGC 
AGTCCAGTCC CGTCCACCTC CGGGACCAGC ACCCCCCGCC GTGGCCGCCG TCAGCTCCCC 
CAAACCCCTG CTACACCCCG GCCACATGTC ACCTACTCAC CTGTGGTCCG TAAGCCCATC 
AGCAGTACCC CCCCTCCTGG CCAGCAGAGC CGACTCCCTA CCCCGACCTC CCGGCGCTTC 
AGTCCGACTG GCCCCGAGCC CCCGCTTCCC CCTCATCACC ACCCGTCACC ACCCCACCAC 
GGCTCTCCCC GCTCCGGCCG ACATGCACAC TGGGGCCCCG AGCCTGCAGA GAGCGTGGAG 
GGTGACGGCT TCTATGACGA CCAAGACTAT GAGTTCAATC ACCACGAGCC TCCATCCTAC 
GAGCAAAGCC CTGTGCAGGG CAACCCTCAC CCCCATTCCC CTAGGACTTC CCGCCACAAC 
ACCCCTCCTC AGGGCCAAGC GCACCCACGG CGCATGCCAA ATGGCTACCG TTCGTCTTCG 
CCCTCTCCAC ACCACCACGC GCCCGCTCAC CCTGGAACTC ATAAACCTCC TCATCCCAGA 
GGGCCCAGAA AGGGCCTCCA TGAACCCTAC AGTGAGACTG ACGAGGATGA CTGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330103.1
CDS	1509..8468
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGGSGGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGMNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGEGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQNRTPLMFQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQQPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_021474428.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474428.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG 
GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT 
GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC 
CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC 
ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT 
GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT 
GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT 
GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT 
CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA 
AGGCAGTCTC AGCAGCCTAT CACAGATAAC AGTCCTATGA AGCGCTCGGC TTCATCTTTG 
GGACACGGGC GTGCAGGACG CTCCATGCGA GGTGAGGATT ATGCCATGGA GAGGGTTATC 
CCAGAAGAGG GTCACAGGCA TGGGCATCGC CACCGAGACC GTAGTCACCG GGCGTCAGAA 
CGCTCCCTGA GCCGATACAC TGATGCTGAC ACAGGTCTTG GAACTGACCT CAGCACAACC 
ACACAATCAG GAGACCTGCC ATCCAAGGAG AGAGACCGGG GCCGAGCCAA AGACCGCAAG 
CACCATCATC ATCATCATCA CCACCACGGC TCTTTAGATA AGGAGCACTA CGGTCATGAG 
AGAGACAGAG GAGAGTACGG CCACAGACAG TCCAGAGAGA GAGACCGCCG CTGGTCCCGC 
TCACCCAGTG AGGGCAGAGA GTGTCTGACT CACAGACAGG GCAGTAGTTC AGTAAGCGGC 
AGTCCAGTCC CGTCCACCTC CGGGACCAGC ACCCCCCGCC GTGGCCGCCG TCAGCTCCCC 
CAAACCCCTG CTACACCCCG GCCACATGTC ACCTACTCAC CTGTGGTCCG TAAGCCCATC 
AGCAGTACCC CCCCTCCTGG CCAGCAGAGC CGACTCCCTA CCCCGACCTC CCGGCGCTTC 
AGTCCGACTG GCCCCGAGCC CCCGCTTCCC CCTCATCACC ACCCGTCACC ACCCCACCAC 
GGCTCTCCCC GCTCCGGCCG ACATGCACAC TGGGGCCCCG AGCCTGCAGA GAGCGTGGAG 
GGTGACGGCT TCTATGACGA CCAAGACTAT GAGTTCAATC ACCACGAGCC TCCATCCTAC 
GAGCAAAGCC CTGTGCAGGG CAACCCTCAC CCCCATTCCC CTAGGACTTC CCGCCACAAC 
ACCCCTCCTC AGGGCCAAGC GCACCCACGG CGCATGCCAA ATGGCTACCG TTCGTCTTCG 
CCCTCTCCAC ACCACCACGC GCCCGCTCAC CCTGGAACTC ATAAACCTCC TCATCCCAGA 
GGGCCCAGAA AGGGCCTCCA TGAACCCTAC AGTGAGACTG ACGAGGATGA CTGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_021474432.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330107.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X22, mRNA.

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CloneID	ODa141800
Clone ID Related Accession (Same CDS sequence)	XM_021474432.1
Accession Version	XM_021474432.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6924bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG 
GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGAGGT GTAGATAAGC ACCAGATGGA TGCTGAATTA 
AGGAAGGAGA TGATGGCGAT ATGGCCTAAT CTCTCTCAGA AGAACCTGGA CCTGCTGGTG 
ACTCCTCACA AGTCGACAGA CCTGACAGTA GGAAAGATCT ACGCTGCCAT GATGATCATG 
GAGTATTATC GGCAAAGTAA AGCCAAACGG ACACAAGCTC TCCATGATGA GCAGCGTCTG 
GAGCCGCCAT CTCCTAGCCA GGATGTGGGA CCAGGACTCA CTGGCCTTCC TGACACACAA 
ATGCACCCTG CAAATCATCT GCCTGTTGAT GAAAGGATCC CAGAGAGCCA ATCATGGGTG 
ACAGCGCGAG CACAGGAGAT TTCTCAAAAA GCAGGCAACT GGAGTCCAGA GGGCCAACAT 
CCAGATGACA CCACAGACAA CAGAAGGCAG TCTCAGCAGC CTATCACAGA TAACAGTCCT 
ATGAAGCGCT CGGCTTCATC TTTGGGACAC GGGCGTGCAG GACGCTCCAT GCGAGGTGAG 
GATTATGCCA TGGAGAGGGT TATCCCAGAA GAGGGTCACA GGCATGGGCA TCGCCACCGA 
GACCGTAGTC ACCGGGCGTC AGAACGCTCC CTGAGCCGAT ACACTGATGC TGACACAGGT 
CTTGGAACTG ACCTCAGCAC AACCACACAA TCAGGAGACC TGCCATCCAA GGAGAGAGAC 
CGGGGCCGAG CCAAAGACCG CAAGCACCAT CATCATCATC ATCACCACCA CGGCTCTTTA 
GATAAGGAGC ACTACGGTCA TGAGAGAGAC AGAGGAGAGT ACGGCCACAG ACAGTCCAGA 
GAGAGAGACC GCCGCTGGTC CCGCTCACCC AGTGAGGGCA GAGAGTGTCT GACTCACAGA 
CAGGGCAGTA GTTCAGTAAG CGGCAGTCCA GTCCCGTCCA CCTCCGGGAC CAGCACCCCC 
CGCCGTGGCC GCCGTCAGCT CCCCCAAACC CCTGCTACAC CCCGGCCACA TGTCACCTAC 
TCACCTGTGG TCCGTAAGCC CATCAGCAGT ACCCCCCCTC CTGGCCAGCA GAGCCGACTC 
CCTACCCCGA CCTCCCGGCG CTTCAGTCCG ACTGGCCCCG AGCCCCCGCT TCCCCCTCAT 
CACCACCCGT CACCACCCCA CCACGGCTCT CCCCGCTCCG GCCGACATGC ACACTGGGGC 
CCCGAGCCTG CAGAGAGCGT GGAGGGTGAC GGCTTCTATG ACGACCAAGA CTATGAGTTC 
AATCACCACG AGCCTCCATC CTACGAGCAA AGCCCTGTGC AGGGCAACCC TCACCCCCAT 
TCCCCTAGGA CTTCCCGCCA CAACACCCCT CCTCAGGGCC AAGCGCACCC ACGGCGCATG 
CCAAATGGCT ACCGTTCGTC TTCGCCCTCT CCACACCACC ACGCGCCCGC TCACCCTGGA 
ACTCATAAAC CTCCTCATCC CAGAGGGCCC AGAAAGGGCC TCCATGAACC CTACAGTGAG 
ACTGACGAGG ATGACTGGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330107.1
CDS	1512..8435
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGGSGGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGMNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQQPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_021474432.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474432.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG 
GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGAGGT GTAGATAAGC ACCAGATGGA TGCTGAATTA 
AGGAAGGAGA TGATGGCGAT ATGGCCTAAT CTCTCTCAGA AGAACCTGGA CCTGCTGGTG 
ACTCCTCACA AGTCGACAGA CCTGACAGTA GGAAAGATCT ACGCTGCCAT GATGATCATG 
GAGTATTATC GGCAAAGTAA AGCCAAACGG ACACAAGCTC TCCATGATGA GCAGCGTCTG 
GAGCCGCCAT CTCCTAGCCA GGATGTGGGA CCAGGACTCA CTGGCCTTCC TGACACACAA 
ATGCACCCTG CAAATCATCT GCCTGTTGAT GAAAGGATCC CAGAGAGCCA ATCATGGGTG 
ACAGCGCGAG CACAGGAGAT TTCTCAAAAA GCAGGCAACT GGAGTCCAGA GGGCCAACAT 
CCAGATGACA CCACAGACAA CAGAAGGCAG TCTCAGCAGC CTATCACAGA TAACAGTCCT 
ATGAAGCGCT CGGCTTCATC TTTGGGACAC GGGCGTGCAG GACGCTCCAT GCGAGGTGAG 
GATTATGCCA TGGAGAGGGT TATCCCAGAA GAGGGTCACA GGCATGGGCA TCGCCACCGA 
GACCGTAGTC ACCGGGCGTC AGAACGCTCC CTGAGCCGAT ACACTGATGC TGACACAGGT 
CTTGGAACTG ACCTCAGCAC AACCACACAA TCAGGAGACC TGCCATCCAA GGAGAGAGAC 
CGGGGCCGAG CCAAAGACCG CAAGCACCAT CATCATCATC ATCACCACCA CGGCTCTTTA 
GATAAGGAGC ACTACGGTCA TGAGAGAGAC AGAGGAGAGT ACGGCCACAG ACAGTCCAGA 
GAGAGAGACC GCCGCTGGTC CCGCTCACCC AGTGAGGGCA GAGAGTGTCT GACTCACAGA 
CAGGGCAGTA GTTCAGTAAG CGGCAGTCCA GTCCCGTCCA CCTCCGGGAC CAGCACCCCC 
CGCCGTGGCC GCCGTCAGCT CCCCCAAACC CCTGCTACAC CCCGGCCACA TGTCACCTAC 
TCACCTGTGG TCCGTAAGCC CATCAGCAGT ACCCCCCCTC CTGGCCAGCA GAGCCGACTC 
CCTACCCCGA CCTCCCGGCG CTTCAGTCCG ACTGGCCCCG AGCCCCCGCT TCCCCCTCAT 
CACCACCCGT CACCACCCCA CCACGGCTCT CCCCGCTCCG GCCGACATGC ACACTGGGGC 
CCCGAGCCTG CAGAGAGCGT GGAGGGTGAC GGCTTCTATG ACGACCAAGA CTATGAGTTC 
AATCACCACG AGCCTCCATC CTACGAGCAA AGCCCTGTGC AGGGCAACCC TCACCCCCAT 
TCCCCTAGGA CTTCCCGCCA CAACACCCCT CCTCAGGGCC AAGCGCACCC ACGGCGCATG 
CCAAATGGCT ACCGTTCGTC TTCGCCCTCT CCACACCACC ACGCGCCCGC TCACCCTGGA 
ACTCATAAAC CTCCTCATCC CAGAGGGCCC AGAAAGGGCC TCCATGAACC CTACAGTGAG 
ACTGACGAGG ATGACTGGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_021474433.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330108.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X23, mRNA.

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CloneID	ODa141801
Clone ID Related Accession (Same CDS sequence)	XM_021474433.1
Accession Version	XM_021474433.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG 
GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT 
GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC 
CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC 
ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT 
GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT 
GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT 
GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT 
CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA 
AGGCAGTCTC AGACAGTAGA GATGAGAGAG ATGGGGCGGG ACGGGTACTC GGACACAGAG 
CAATATCTGC CGATGGAAGG CCATGGCAGG GCTGCTTCCA TGCCTCGACT CCCAGCAGAC 
AACCAACAGC CACGGAGGAA AGGGCGTCAC CGGGGAGAAA ATCTCACTCA GCCTATCACA 
GATAACAGTC CTATGAAGCG CTCGGCTTCA TCTTTGGGAC ACGGGCGTGC AGGACGCTCC 
ATGCGAGGTG AGGATTATGC CATGGAGAGG GTTATCCCAG AAGAGGGTCA CAGGCATGGG 
CATCGCCACC GAGACCGTAG TCACCGGGCG TCAGAACGCT CCCTGAGCCG ATACACTGAT 
GCTGACACAG GTCTTGGAAC TGACCTCAGC ACAACCACAC AATCAGGAGA CCTGCCATCC 
AAGGAGAGAG ACCGGGGCCG AGCCAAAGAC CGCAAGCACC ATCATCATCA TCATCACCAC 
CACGGCTCTT TAGATAAGGA GCACTACGGT CATGAGAGAG ACAGAGGAGA GTACGGCCAC 
AGACAGTCCA GAGAGAGAGA CCGCCGCTGG TCCCGCTCAC CCAGTGAGGG CAGAGAGTGT 
CTGACTCACA GACAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330108.1
CDS	1519..8016
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGGSGGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGMNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGEGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQNRTPLMFQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQTVEMREMGRDGYSDTEQYLPMEGHGRAASMPRLPADNQQPRRKGRHRGENLTQPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQ

Target ORF information:

RefSeq Version	XM_021474433.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474433.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG 
GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT 
GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC 
CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC 
ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT 
GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT 
GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT 
GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT 
CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA 
AGGCAGTCTC AGACAGTAGA GATGAGAGAG ATGGGGCGGG ACGGGTACTC GGACACAGAG 
CAATATCTGC CGATGGAAGG CCATGGCAGG GCTGCTTCCA TGCCTCGACT CCCAGCAGAC 
AACCAACAGC CACGGAGGAA AGGGCGTCAC CGGGGAGAAA ATCTCACTCA GCCTATCACA 
GATAACAGTC CTATGAAGCG CTCGGCTTCA TCTTTGGGAC ACGGGCGTGC AGGACGCTCC 
ATGCGAGGTG AGGATTATGC CATGGAGAGG GTTATCCCAG AAGAGGGTCA CAGGCATGGG 
CATCGCCACC GAGACCGTAG TCACCGGGCG TCAGAACGCT CCCTGAGCCG ATACACTGAT 
GCTGACACAG GTCTTGGAAC TGACCTCAGC ACAACCACAC AATCAGGAGA CCTGCCATCC 
AAGGAGAGAG ACCGGGGCCG AGCCAAAGAC CGCAAGCACC ATCATCATCA TCATCACCAC 
CACGGCTCTT TAGATAAGGA GCACTACGGT CATGAGAGAG ACAGAGGAGA GTACGGCCAC 
AGACAGTCCA GAGAGAGAGA CCGCCGCTGG TCCCGCTCAC CCAGTGAGGG CAGAGAGTGT 
CTGACTCACA GACAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_021474440.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330115.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X25, mRNA.

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CloneID	ODa141802
Clone ID Related Accession (Same CDS sequence)	XM_021474440.1
Accession Version	XM_021474440.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGT TTTTTTGTTA TAATCAACCT 
TTATTTGGAT CCAGAGTTTG TTGTAAGATA TGCATAATGA TAATTCATTT CTTGTTTCAT 
CTTTTTTTAC ATGCTTTCTT TAAACTCTTC TTATTGGCGG CTTCGCCCTC TGATGACTCC 
ATCCAATCAA AATGCACTAT GAAACTCATG TGTATATCCA TGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330115.1
CDS	1556..6403
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGGSGGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGFFCYNQPLFGSRVCCKICIMIIHFLFHLFLHAFFKLFLLAASPSDDSIQSKCTMKLMCISMT

Target ORF information:

RefSeq Version	XM_021474440.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474440.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGT TTTTTTGTTA TAATCAACCT 
TTATTTGGAT CCAGAGTTTG TTGTAAGATA TGCATAATGA TAATTCATTT CTTGTTTCAT 
CTTTTTTTAC ATGCTTTCTT TAAACTCTTC TTATTGGCGG CTTCGCCCTC TGATGACTCC 
ATCCAATCAA AATGCACTAT GAAACTCATG TGTATATCCA TGACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_021474441.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330116.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X27, mRNA.

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CloneID	ODa141803
Clone ID Related Accession (Same CDS sequence)	XM_021474441.1
Accession Version	XM_021474441.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7035bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCTAAGGA GCAGCAGAAG AACCACAAAG GTTGTAAGTC GGTATGGGAG 
CAGCGCACTA GTGAGTTAAG ACGACAGACC CTCGTGAACA GCAGAGAGGC TCTTTATAAT 
GAGTTGGACC CTGAAGACCG TTGGAAGGTC TCATACTCCC GTCACATACG TCCCGACATG 
AAAACTCACC TGGACCGGCC GTTAGTCGTG GACCCTCAGG AGAACCGCAA TAACAACACT 
AACAAAACCC GTCCGGGTGA CGGCCAACCT CAGCATTCCC ATCAGCTCCT ACACAAACAG 
CCCAACTTCA ATGAGGCCGA GACTGGAGGA GGTGGAGGGT CTGAGTCCTG GCCCGCACTG 
GAAAGAGGAG ACTCAACAGG GTCCCGCCGC AGTCTTTTGG GCTCTGAGCA CTATGAGGAG 
GGTAGAGAGT CCCACCGCAG ACACCAGCAC AGCCAGCACT GCAGTCAGGC CCGTCAACAT 
CGCAAAGCTG CAGAGGGCGG TGGAGAGCCT GGAGGGAGGA GACACAGGAG TAAGGCCAAG 
GAAAGAGGAC CTGAGTGTGA GCATTCAGAT GGAGGGGAGC GGAAACACAG ACGGCATCGG 
CATGAGGGTG AGGGGAGGAA AGAGAGAGGG GTACGACACC GAAACAGGAG GGATGGCCAT 
GCAAGTGGCC CGACTCTCTC TACAACCCGT CCCATTCAGA AGACTCTGTC CAGGCAGGAC 
AGTCAGTACA GCGAAGACCT GGACAATGCC ATGAACAACA AACTTGCTAC CCAGCCACAT 
GATAGTCTGC TTAATCTGGC CAACACTGCC CACTCTGCCG GCCTGGCACA TACTGGTACA 
GAGAGCAGCC TCATCCTCAC CAACCCTTCC TCTACCATTG CAAATGTTAC TAGCATCGGT 
CACCTGGGTA TGAAACCTGA GTACACTGCC GTGGACATTC CTCCCATGTT CCCCTCCAGT 
AATGCCATTC TACAGGTCAA TAAGAATGCC AACACGGAGC CTCTGCCAAA GAAGGAAGAC 
ACAAAGGGTG ACGATGATGA TGATGAGAAA GATGACGGAG GGCCTAAACC CATGCCACCC 
TACACCTCAA TGTTCATTCT GACCACCACC AACCCATTTC GGAGATTGTG TCACTATATC 
GTCACACTGA GATATTTTGA GATGTGTATT CTACTGGTTA TTGCTATGAG CAGTATTGCC 
CTGGCTGCTG AAGACCCTGT CTGGCCTGAA TCTCCCCGCA ACAATGTCCT ACGATACTTT 
GACTATGTGT TCACTGGTGT GTTCACCTTT GAGATGCTCA TTAAGATGGT AGATTTAGGT 
CTTGTCCTGC ATCAAGGCTC ATACTTTCGT GATCTGTGGA ACATCCTGGA CTTTATAGTG 
GTTAGTGGTG CCCTGGTGGC GTTCGCCTTC ACAGGAAGCA GCAAAGGGAA AGACATCAGT 
ACTATCAAGT CTCTACGTGT GCTTCGAGTA CTGCGGCCGC TGAAAACCAT CAAACGTCTG 
CCAAAACTGA AGGCTGTCTT TGACTGTGTG GTGAACTCTC TGAAGAATGT GTTGAACATC 
CTCATCGTCT ACATGCTCTT CATGTTCATC TTCGCTGTGG TGGCTGTGCA GCTCTTTAAG 
GGACGTTTCT TTTACTGCAC TGATGAATCC AAAGAGTTTG AGCGCGACTG CAGGGGCGAG 
TATCTGGTCT ATGAAAGAGA TAACGAAGTG AGGTCACAGA AGCGGGAATG GAAGAAATAC 
GATTTCCACT ACGACAACGT GCTCTGGGCC CTTCTCACCC TCTTCACCGT TTCTACGGGA 
GAGGGGTGGC CGCAAGTGCT AAAACATTCA GTAGATGCGA CCTACGAGAA TCAGGGCCCG 
AGCCCAGGTT ACCGGATGGA AATGTCCATC TTTTACGTGG TGTACTTCGT GGTCTTCCCC 
TTCTTCTTCG TCAACATCTT CGTGGCTCTT ATCATCATTA CTTTCCAGGA GCAAGGAGAC 
AAAATGATGG AGGACTATAG CCTGGAAAAG AATGAGAGAG CCTGCATAGA CTTTGCCATC 
AACGCTAAGC CGCTCACACG GCACATGCCC CAGAACAAGC AGACCTTTCA GTACCGCATG 
TGGGAGTTTG TGGTGTCTCC TCCATTTGAA TACACCATCA TGGCCTTGAT CGCGCTCAAC 
ACTATTGTCC TTATGATGAA GTATGATGGA GCATCGCTTA CTTATGAAGA TGTGCTAAAA 
TACCTGAACA TAGTGTTCAC CTCTCTATTC TCCATGGAGT GCATATTGAA GATCATAGCC 
TTCGGAGCAT TGAACTACTT CAAAGATGCC TGGAACATCT TCGACTGTGT GACTGTACTT 
GGCAGCATCA CTGATATTTT AGTCACAGAG CTGGGGAACA ACTTCATAAA TCTGAGTTTT 
CTAAGGCTCT TCAGAGCTGC TCGTCTCATT AAGTTGCTCA GGCAGGGTGA AACCATACGC 
ATCCTGCTCT GGACTTTTGT CCAGTCTTTC AAGGCTCTGC CGTATGTGTG CCTACTCATC 
GCCATGCTGT TCTTCATCTA CGCCATCATT GGCATGCAGC TGTTTGGGAA CATTAAGATC 
GAGGAGAACA GTGACAGTGC CATTACCCAG CACAACAACT TCAGGACTTT TTTCCAGGCC 
CTAATGCTGC TCTTTAGGAG TGCTACAGGT GAGGCATGGC ATGACATCAT GCTGTCATGT 
CTGGGAAAGA AGCCGTGTGA TATTCTCTCT GATAACCCGA AGCCAGAATG CGGGAGCGAG 
TTTGCCTACC TCTACTTTGT CTCCTTCATC TTCCTCTGTT CATTTCTGAT GCTGAATCTG 
TTCGTGGCTG TGATCATGGA TAATTTTGAA TACCTGACGC GTGACTCCTC TATCCTGGGA 
CCCCATCACC TGGACGAGTA TGTGAGGATC TGGGCAGAAT ATGATCCTGC TGCTTGTGGC 
AGAATCAGTT ACAGGGATAT GTATGAGATG CTCAGGCACA TGTCTCCTCC GTTAGGCCTG 
GGGAAGAAGT GCCCTGCACG GGTTGCCTAC AAGAGACTGT TGCGGATGGA TCTGCCGGTT 
GCCGATGACA ACACAGTGCA CTTTAATTCT ACCCTTATGG CCTTAATCCG AACCGCTCTG 
GACATCAAGA TCGCCAAGGG AGGTGTAGAT AAGCACCAGA TGGATGCTGA ATTAAGGAAG 
GAGATGATGG CGATATGGCC TAATCTCTCT CAGAAGAACC TGGACCTGCT GGTGACTCCT 
CACAAGTCGA CAGACCTGAC AGTAGGAAAG ATCTACGCTG CCATGATGAT CATGGAGTAT 
TATCGGCAAA GTAAAGCCAA ACGGACACAA GCTCTCCATG ATGAGCAGAA TCGAACGCCA 
TTAATGTTTC AGCGTCTGGA GCCGCCATCT CCTAGCCAGG ATGTGGGACC AGGACTCACT 
GGCCTTCCTG ACACACAAAT GCACCCTGCA AATCATCTGC CTGTTGATGA AAGGATCCCA 
GAGAGCCAAT CATGGGTGAC AGCGCGAGCA CAGGAGATTT CTCAAAAAGC AGGCAACTGG 
AGTCCAGAGG GCCAACATCC AGATGACACC ACAGACAACA GAAGGCAGTC TCAGACAGTA 
GAGATGAGAG AGATGGGGCG GGACGGGTAC TCGGACACAG AGCAATATCT GCCGATGGAA 
GGCCATGGCA GGGCTGCTTC CATGCCTCGA CTCCCAGCAG ACAACCAACA GCCACGGAGG 
AAAGGGCGTC ACCGGGGAGA AAATCTCACT CCTATCACAG ATAACAGTCC TATGAAGCGC 
TCGGCTTCAT CTTTGGGACA CGGGCGTGCA GGACGCTCCA TGCGAGGTGA GGATTATGCC 
ATGGAGAGGG TTATCCCAGA AGAGGGTCAC AGGCATGGGC ATCGCCACCG AGACCGTAGT 
CACCGGGCGT CAGAACGCTC CCTGAGCCGA TACACTGATG CTGACACAGG TCTTGGAACT 
GACCTCAGCA CAACCACACA ATCAGGAGAC CTGCCATCCA AGGAGAGAGA CCGGGGCCGA 
GCCAAAGACC GCAAGCACCA TCATCATCAT CATCACCACC ACGGCTCTTT AGATAAGGAG 
CACTACGGTC ATGAGAGAGA CAGAGGAGAG TACGGCCACA GACAGTCCAG AGAGAGAGAC 
CGCCGCTGGT CCCGCTCACC CAGTGAGGGC AGAGAGTGTC TGACTCACAG ACAGGGCAGT 
AGTTCAGTAA GCGGCAGTCC AGTCCCGTCC ACCTCCGGGA CCAGCACCCC CCGCCGTGGC 
CGCCGTCAGC TCCCCCAAAC CCCTGCTACA CCCCGGCCAC ATGTCACCTA CTCACCTGTG 
GTCCGTAAGC CCATCAGCAG TACCCCCCCT CCTGGCCAGC AGAGCCGACT CCCTACCCCG 
ACCTCCCGGC GCTTCAGTCC GACTGGCCCC GAGCCCCCGC TTCCCCCTCA TCACCACCCG 
TCACCACCCC ACCACGGCTC TCCCCGCTCC GGCCGACATG CACACTGGGG CCCCGAGCCT 
GCAGAGAGCG TGGAGGGTGA CGGCTTCTAT GACGACCAAG ACTATGAGTT CAATCACCAC 
GAGCCTCCAT CCTACGAGCA AAGCCCTGTG CAGGGCAACC CTCACCCCCA TTCCCCTAGG 
ACTTCCCGCC ACAACACCCC TCCTCAGGGC CAAGCGCACC CACGGCGCAT GCCAAATGGC 
TACCGTTCGT CTTCGCCCTC TCCACACCAC CACGCGCCCG CTCACCCTGG AACTCATAAA 
CCTCCTCATC CCAGAGGGCC CAGAAAGGGC CTCCATGAAC CCTACAGTGA GACTGACGAG 
GATGACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330116.1
CDS	1508..8542
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRISYRDMYEMLRHMSPPLGLGKKCPARVAYKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQNRTPLMFQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQTVEMREMGRDGYSDTEQYLPMEGHGRAASMPRLPADNQQPRRKGRHRGENLTPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_021474441.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474441.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCTAAGGA GCAGCAGAAG AACCACAAAG GTTGTAAGTC GGTATGGGAG 
CAGCGCACTA GTGAGTTAAG ACGACAGACC CTCGTGAACA GCAGAGAGGC TCTTTATAAT 
GAGTTGGACC CTGAAGACCG TTGGAAGGTC TCATACTCCC GTCACATACG TCCCGACATG 
AAAACTCACC TGGACCGGCC GTTAGTCGTG GACCCTCAGG AGAACCGCAA TAACAACACT 
AACAAAACCC GTCCGGGTGA CGGCCAACCT CAGCATTCCC ATCAGCTCCT ACACAAACAG 
CCCAACTTCA ATGAGGCCGA GACTGGAGGA GGTGGAGGGT CTGAGTCCTG GCCCGCACTG 
GAAAGAGGAG ACTCAACAGG GTCCCGCCGC AGTCTTTTGG GCTCTGAGCA CTATGAGGAG 
GGTAGAGAGT CCCACCGCAG ACACCAGCAC AGCCAGCACT GCAGTCAGGC CCGTCAACAT 
CGCAAAGCTG CAGAGGGCGG TGGAGAGCCT GGAGGGAGGA GACACAGGAG TAAGGCCAAG 
GAAAGAGGAC CTGAGTGTGA GCATTCAGAT GGAGGGGAGC GGAAACACAG ACGGCATCGG 
CATGAGGGTG AGGGGAGGAA AGAGAGAGGG GTACGACACC GAAACAGGAG GGATGGCCAT 
GCAAGTGGCC CGACTCTCTC TACAACCCGT CCCATTCAGA AGACTCTGTC CAGGCAGGAC 
AGTCAGTACA GCGAAGACCT GGACAATGCC ATGAACAACA AACTTGCTAC CCAGCCACAT 
GATAGTCTGC TTAATCTGGC CAACACTGCC CACTCTGCCG GCCTGGCACA TACTGGTACA 
GAGAGCAGCC TCATCCTCAC CAACCCTTCC TCTACCATTG CAAATGTTAC TAGCATCGGT 
CACCTGGGTA TGAAACCTGA GTACACTGCC GTGGACATTC CTCCCATGTT CCCCTCCAGT 
AATGCCATTC TACAGGTCAA TAAGAATGCC AACACGGAGC CTCTGCCAAA GAAGGAAGAC 
ACAAAGGGTG ACGATGATGA TGATGAGAAA GATGACGGAG GGCCTAAACC CATGCCACCC 
TACACCTCAA TGTTCATTCT GACCACCACC AACCCATTTC GGAGATTGTG TCACTATATC 
GTCACACTGA GATATTTTGA GATGTGTATT CTACTGGTTA TTGCTATGAG CAGTATTGCC 
CTGGCTGCTG AAGACCCTGT CTGGCCTGAA TCTCCCCGCA ACAATGTCCT ACGATACTTT 
GACTATGTGT TCACTGGTGT GTTCACCTTT GAGATGCTCA TTAAGATGGT AGATTTAGGT 
CTTGTCCTGC ATCAAGGCTC ATACTTTCGT GATCTGTGGA ACATCCTGGA CTTTATAGTG 
GTTAGTGGTG CCCTGGTGGC GTTCGCCTTC ACAGGAAGCA GCAAAGGGAA AGACATCAGT 
ACTATCAAGT CTCTACGTGT GCTTCGAGTA CTGCGGCCGC TGAAAACCAT CAAACGTCTG 
CCAAAACTGA AGGCTGTCTT TGACTGTGTG GTGAACTCTC TGAAGAATGT GTTGAACATC 
CTCATCGTCT ACATGCTCTT CATGTTCATC TTCGCTGTGG TGGCTGTGCA GCTCTTTAAG 
GGACGTTTCT TTTACTGCAC TGATGAATCC AAAGAGTTTG AGCGCGACTG CAGGGGCGAG 
TATCTGGTCT ATGAAAGAGA TAACGAAGTG AGGTCACAGA AGCGGGAATG GAAGAAATAC 
GATTTCCACT ACGACAACGT GCTCTGGGCC CTTCTCACCC TCTTCACCGT TTCTACGGGA 
GAGGGGTGGC CGCAAGTGCT AAAACATTCA GTAGATGCGA CCTACGAGAA TCAGGGCCCG 
AGCCCAGGTT ACCGGATGGA AATGTCCATC TTTTACGTGG TGTACTTCGT GGTCTTCCCC 
TTCTTCTTCG TCAACATCTT CGTGGCTCTT ATCATCATTA CTTTCCAGGA GCAAGGAGAC 
AAAATGATGG AGGACTATAG CCTGGAAAAG AATGAGAGAG CCTGCATAGA CTTTGCCATC 
AACGCTAAGC CGCTCACACG GCACATGCCC CAGAACAAGC AGACCTTTCA GTACCGCATG 
TGGGAGTTTG TGGTGTCTCC TCCATTTGAA TACACCATCA TGGCCTTGAT CGCGCTCAAC 
ACTATTGTCC TTATGATGAA GTATGATGGA GCATCGCTTA CTTATGAAGA TGTGCTAAAA 
TACCTGAACA TAGTGTTCAC CTCTCTATTC TCCATGGAGT GCATATTGAA GATCATAGCC 
TTCGGAGCAT TGAACTACTT CAAAGATGCC TGGAACATCT TCGACTGTGT GACTGTACTT 
GGCAGCATCA CTGATATTTT AGTCACAGAG CTGGGGAACA ACTTCATAAA TCTGAGTTTT 
CTAAGGCTCT TCAGAGCTGC TCGTCTCATT AAGTTGCTCA GGCAGGGTGA AACCATACGC 
ATCCTGCTCT GGACTTTTGT CCAGTCTTTC AAGGCTCTGC CGTATGTGTG CCTACTCATC 
GCCATGCTGT TCTTCATCTA CGCCATCATT GGCATGCAGC TGTTTGGGAA CATTAAGATC 
GAGGAGAACA GTGACAGTGC CATTACCCAG CACAACAACT TCAGGACTTT TTTCCAGGCC 
CTAATGCTGC TCTTTAGGAG TGCTACAGGT GAGGCATGGC ATGACATCAT GCTGTCATGT 
CTGGGAAAGA AGCCGTGTGA TATTCTCTCT GATAACCCGA AGCCAGAATG CGGGAGCGAG 
TTTGCCTACC TCTACTTTGT CTCCTTCATC TTCCTCTGTT CATTTCTGAT GCTGAATCTG 
TTCGTGGCTG TGATCATGGA TAATTTTGAA TACCTGACGC GTGACTCCTC TATCCTGGGA 
CCCCATCACC TGGACGAGTA TGTGAGGATC TGGGCAGAAT ATGATCCTGC TGCTTGTGGC 
AGAATCAGTT ACAGGGATAT GTATGAGATG CTCAGGCACA TGTCTCCTCC GTTAGGCCTG 
GGGAAGAAGT GCCCTGCACG GGTTGCCTAC AAGAGACTGT TGCGGATGGA TCTGCCGGTT 
GCCGATGACA ACACAGTGCA CTTTAATTCT ACCCTTATGG CCTTAATCCG AACCGCTCTG 
GACATCAAGA TCGCCAAGGG AGGTGTAGAT AAGCACCAGA TGGATGCTGA ATTAAGGAAG 
GAGATGATGG CGATATGGCC TAATCTCTCT CAGAAGAACC TGGACCTGCT GGTGACTCCT 
CACAAGTCGA CAGACCTGAC AGTAGGAAAG ATCTACGCTG CCATGATGAT CATGGAGTAT 
TATCGGCAAA GTAAAGCCAA ACGGACACAA GCTCTCCATG ATGAGCAGAA TCGAACGCCA 
TTAATGTTTC AGCGTCTGGA GCCGCCATCT CCTAGCCAGG ATGTGGGACC AGGACTCACT 
GGCCTTCCTG ACACACAAAT GCACCCTGCA AATCATCTGC CTGTTGATGA AAGGATCCCA 
GAGAGCCAAT CATGGGTGAC AGCGCGAGCA CAGGAGATTT CTCAAAAAGC AGGCAACTGG 
AGTCCAGAGG GCCAACATCC AGATGACACC ACAGACAACA GAAGGCAGTC TCAGACAGTA 
GAGATGAGAG AGATGGGGCG GGACGGGTAC TCGGACACAG AGCAATATCT GCCGATGGAA 
GGCCATGGCA GGGCTGCTTC CATGCCTCGA CTCCCAGCAG ACAACCAACA GCCACGGAGG 
AAAGGGCGTC ACCGGGGAGA AAATCTCACT CCTATCACAG ATAACAGTCC TATGAAGCGC 
TCGGCTTCAT CTTTGGGACA CGGGCGTGCA GGACGCTCCA TGCGAGGTGA GGATTATGCC 
ATGGAGAGGG TTATCCCAGA AGAGGGTCAC AGGCATGGGC ATCGCCACCG AGACCGTAGT 
CACCGGGCGT CAGAACGCTC CCTGAGCCGA TACACTGATG CTGACACAGG TCTTGGAACT 
GACCTCAGCA CAACCACACA ATCAGGAGAC CTGCCATCCA AGGAGAGAGA CCGGGGCCGA 
GCCAAAGACC GCAAGCACCA TCATCATCAT CATCACCACC ACGGCTCTTT AGATAAGGAG 
CACTACGGTC ATGAGAGAGA CAGAGGAGAG TACGGCCACA GACAGTCCAG AGAGAGAGAC 
CGCCGCTGGT CCCGCTCACC CAGTGAGGGC AGAGAGTGTC TGACTCACAG ACAGGGCAGT 
AGTTCAGTAA GCGGCAGTCC AGTCCCGTCC ACCTCCGGGA CCAGCACCCC CCGCCGTGGC 
CGCCGTCAGC TCCCCCAAAC CCCTGCTACA CCCCGGCCAC ATGTCACCTA CTCACCTGTG 
GTCCGTAAGC CCATCAGCAG TACCCCCCCT CCTGGCCAGC AGAGCCGACT CCCTACCCCG 
ACCTCCCGGC GCTTCAGTCC GACTGGCCCC GAGCCCCCGC TTCCCCCTCA TCACCACCCG 
TCACCACCCC ACCACGGCTC TCCCCGCTCC GGCCGACATG CACACTGGGG CCCCGAGCCT 
GCAGAGAGCG TGGAGGGTGA CGGCTTCTAT GACGACCAAG ACTATGAGTT CAATCACCAC 
GAGCCTCCAT CCTACGAGCA AAGCCCTGTG CAGGGCAACC CTCACCCCCA TTCCCCTAGG 
ACTTCCCGCC ACAACACCCC TCCTCAGGGC CAAGCGCACC CACGGCGCAT GCCAAATGGC 
TACCGTTCGT CTTCGCCCTC TCCACACCAC CACGCGCCCG CTCACCCTGG AACTCATAAA 
CCTCCTCATC CCAGAGGGCC CAGAAAGGGC CTCCATGAAC CCTACAGTGA GACTGACGAG 
GATGACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_021474442.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330117.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X28, mRNA.

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CloneID	ODa141804
Clone ID Related Accession (Same CDS sequence)	XM_021474442.1
Accession Version	XM_021474442.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7035bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCTAAGGA GCAGCAGAAG AACCACAAAG GTTGTAAGTC GGTATGGGAG 
CAGCGCACTA GTGAGTTAAG ACGACAGACC CTCGTGAACA GCAGAGAGGC TCTTTATAAT 
GAGTTGGACC CTGAAGACCG TTGGAAGGTC TCATACTCCC GTCACATACG TCCCGACATG 
AAAACTCACC TGGACCGGCC GTTAGTCGTG GACCCTCAGG AGAACCGCAA TAACAACACT 
AACAAAACCC GTCCGGGTGA CGGCCAACCT CAGCATTCCC ATCAGCTCCT ACACAAACAG 
CCCAACTTCA ATGAGGCCGA GACTGGAGGA GGTGGAGGGT CTGAGTCCTG GCCCGCACTG 
GAAAGAGGAG ACTCAACAGG GTCCCGCCGC AGTCTTTTGG GCTCTGAGCA CTATGAGGAG 
GGTAGAGAGT CCCACCGCAG ACACCAGCAC AGCCAGCACT GCAGTCAGGC CCGTCAACAT 
CGCAAAGCTG CAGAGGGCGG TGGAGAGCCT GGAGGGAGGA GACACAGGAG TAAGGCCAAG 
GAAAGAGGAC CTGAGTGTGA GCATTCAGAT GGAGGGGAGC GGAAACACAG ACGGCATCGG 
CATGAGGGTG AGGGGAGGAA AGAGAGAGGG GTACGACACC GAAACAGGAG GGATGGCCAT 
GCAAGTGGCC CGACTCTCTC TACAACCCGT CCCATTCAGA AGACTCTGTC CAGGCAGGAC 
AGTCAGTACA GCGAAGACCT GGACAATGCC ATGAACAACA AACTTGCTAC CCAGCCACAT 
GATAGTCTGC TTAATCTGGC CAACACTGCC CACTCTGCCG GCCTGGCACA TACTGGTACA 
GAGAGCAGCC TCATCCTCAC CAACCCTTCC TCTACCATTG CAAATGTTAC TAGCATCGGT 
CACCTGGGTA TGAAACCTGA GTACACTGCC GTGGACATTC CTCCCATGTT CCCCTCCAGT 
AATGCCATTC TACAGGTCAA TAAGAATGCC AACACGGAGC CTCTGCCAAA GAAGGAAGAC 
ACAAAGGGTG ACGATGATGA TGATGAGAAA GATGACGGAG GGCCTAAACC CATGCCACCC 
TACACCTCAA TGTTCATTCT GACCACCACC AACCCATTTC GGAGATTGTG TCACTATATC 
GTCACACTGA GATATTTTGA GATGTGTATT CTACTGGTTA TTGCTATGAG CAGTATTGCC 
CTGGCTGCTG AAGACCCTGT CTGGCCTGAA TCTCCCCGCA ACAATGTCCT ACGATACTTT 
GACTATGTGT TCACTGGTGT GTTCACCTTT GAGATGCTCA TTAAGATGGT AGATTTAGGT 
CTTGTCCTGC ATCAAGGCTC ATACTTTCGT GATCTGTGGA ACATCCTGGA CTTTATAGTG 
GTTAGTGGTG CCCTGGTGGC GTTCGCCTTC ACAGGAAGCA GCAAAGGGAA AGACATCAGT 
ACTATCAAGT CTCTACGTGT GCTTCGAGTA CTGCGGCCGC TGAAAACCAT CAAACGTCTG 
CCAAAACTGA AGGCTGTCTT TGACTGTGTG GTGAACTCTC TGAAGAATGT GTTGAACATC 
CTCATCGTCT ACATGCTCTT CATGTTCATC TTCGCTGTGG TGGCTGTGCA GCTCTTTAAG 
GGACGTTTCT TTTACTGCAC TGATGAATCC AAAGAGTTTG AGCGCGACTG CAGGGGCGAG 
TATCTGGTCT ATGAAAGAGA TAACGAAGTG AGGTCACAGA AGCGGGAATG GAAGAAATAC 
GATTTCCACT ACGACAACGT GCTCTGGGCC CTTCTCACCC TCTTCACCGT TTCTACGGGA 
GAGGGGTGGC CGCAAGTGCT AAAACATTCA GTAGATGCGA CCTACGAGAA TCAGGGCCCG 
AGCCCAGGTT ACCGGATGGA AATGTCCATC TTTTACGTGG TGTACTTCGT GGTCTTCCCC 
TTCTTCTTCG TCAACATCTT CGTGGCTCTT ATCATCATTA CTTTCCAGGA GCAAGGAGAC 
AAAATGATGG AGGACTATAG CCTGGAAAAG AATGAGAGAG CCTGCATAGA CTTTGCCATC 
AACGCTAAGC CGCTCACACG GCACATGCCC CAGAACAAGC AGACCTTTCA GTACCGCATG 
TGGGAGTTTG TGGTGTCTCC TCCATTTGAA TACACCATCA TGGCCTTGAT CGCGCTCAAC 
ACTATTGTCC TTATGATGAA GTATGATGGA GCATCGCTTA CTTATGAAGA TGTGCTAAAA 
TACCTGAACA TAGTGTTCAC CTCTCTATTC TCCATGGAGT GCATATTGAA GATCATAGCC 
TTCGGAGCAT TGAACTACTT CAAAGATGCC TGGAACATCT TCGACTGTGT GACTGTACTT 
GGCAGCATCA CTGATATTTT AGTCACAGAG CTGGGGAACA ACTTCATAAA TCTGAGTTTT 
CTAAGGCTCT TCAGAGCTGC TCGTCTCATT AAGTTGCTCA GGCAGGGTGA AACCATACGC 
ATCCTGCTCT GGACTTTTGT CCAGTCTTTC AAGGCTCTGC CGTATGTGTG CCTACTCATC 
GCCATGCTGT TCTTCATCTA CGCCATCATT GGCATGCAGC TGTTTGGGAA CATTAAGATC 
GAGGAGAACA GTGACAGTGC CATTACCCAG CACAACAACT TCAGGACTTT TTTCCAGGCC 
CTAATGCTGC TCTTTAGGAG TGCTACAGGT GAGGCATGGC ATGACATCAT GCTGTCATGT 
CTGGGAAAGA AGCCGTGTGA TATTCTCTCT GATAACCCGA AGCCAGAATG CGGGAGCGAG 
TTTGCCTACC TCTACTTTGT CTCCTTCATC TTCCTCTGTT CATTTCTGAT GCTGAATCTG 
TTCGTGGCTG TGATCATGGA TAATTTTGAA TACCTGACGC GTGACTCCTC TATCCTGGGA 
CCCCATCACC TGGACGAGTA TGTGAGGATC TGGGCAGAAT ATGATCCTGC TGCTTGCGGG 
CGCATTCATT ATAAGGATAT GTACAGTTTA TTGCGAGTTA TCGACCCCCC TCTGGGCTTA 
GGCAAGAAAT GTCCTCATAG GGTGGCCTGC AAGAGACTGT TGCGGATGGA TCTGCCGGTT 
GCCGATGACA ACACAGTGCA CTTTAATTCT ACCCTTATGG CCTTAATCCG AACCGCTCTG 
GACATCAAGA TCGCCAAGGG AGGTGTAGAT AAGCACCAGA TGGATGCTGA ATTAAGGAAG 
GAGATGATGG CGATATGGCC TAATCTCTCT CAGAAGAACC TGGACCTGCT GGTGACTCCT 
CACAAGTCGA CAGACCTGAC AGTAGGAAAG ATCTACGCTG CCATGATGAT CATGGAGTAT 
TATCGGCAAA GTAAAGCCAA ACGGACACAA GCTCTCCATG ATGAGCAGAA TCGAACGCCA 
TTAATGTTTC AGCGTCTGGA GCCGCCATCT CCTAGCCAGG ATGTGGGACC AGGACTCACT 
GGCCTTCCTG ACACACAAAT GCACCCTGCA AATCATCTGC CTGTTGATGA AAGGATCCCA 
GAGAGCCAAT CATGGGTGAC AGCGCGAGCA CAGGAGATTT CTCAAAAAGC AGGCAACTGG 
AGTCCAGAGG GCCAACATCC AGATGACACC ACAGACAACA GAAGGCAGTC TCAGACAGTA 
GAGATGAGAG AGATGGGGCG GGACGGGTAC TCGGACACAG AGCAATATCT GCCGATGGAA 
GGCCATGGCA GGGCTGCTTC CATGCCTCGA CTCCCAGCAG ACAACCAACA GCCACGGAGG 
AAAGGGCGTC ACCGGGGAGA AAATCTCACT CCTATCACAG ATAACAGTCC TATGAAGCGC 
TCGGCTTCAT CTTTGGGACA CGGGCGTGCA GGACGCTCCA TGCGAGGTGA GGATTATGCC 
ATGGAGAGGG TTATCCCAGA AGAGGGTCAC AGGCATGGGC ATCGCCACCG AGACCGTAGT 
CACCGGGCGT CAGAACGCTC CCTGAGCCGA TACACTGATG CTGACACAGG TCTTGGAACT 
GACCTCAGCA CAACCACACA ATCAGGAGAC CTGCCATCCA AGGAGAGAGA CCGGGGCCGA 
GCCAAAGACC GCAAGCACCA TCATCATCAT CATCACCACC ACGGCTCTTT AGATAAGGAG 
CACTACGGTC ATGAGAGAGA CAGAGGAGAG TACGGCCACA GACAGTCCAG AGAGAGAGAC 
CGCCGCTGGT CCCGCTCACC CAGTGAGGGC AGAGAGTGTC TGACTCACAG ACAGGGCAGT 
AGTTCAGTAA GCGGCAGTCC AGTCCCGTCC ACCTCCGGGA CCAGCACCCC CCGCCGTGGC 
CGCCGTCAGC TCCCCCAAAC CCCTGCTACA CCCCGGCCAC ATGTCACCTA CTCACCTGTG 
GTCCGTAAGC CCATCAGCAG TACCCCCCCT CCTGGCCAGC AGAGCCGACT CCCTACCCCG 
ACCTCCCGGC GCTTCAGTCC GACTGGCCCC GAGCCCCCGC TTCCCCCTCA TCACCACCCG 
TCACCACCCC ACCACGGCTC TCCCCGCTCC GGCCGACATG CACACTGGGG CCCCGAGCCT 
GCAGAGAGCG TGGAGGGTGA CGGCTTCTAT GACGACCAAG ACTATGAGTT CAATCACCAC 
GAGCCTCCAT CCTACGAGCA AAGCCCTGTG CAGGGCAACC CTCACCCCCA TTCCCCTAGG 
ACTTCCCGCC ACAACACCCC TCCTCAGGGC CAAGCGCACC CACGGCGCAT GCCAAATGGC 
TACCGTTCGT CTTCGCCCTC TCCACACCAC CACGCGCCCG CTCACCCTGG AACTCATAAA 
CCTCCTCATC CCAGAGGGCC CAGAAAGGGC CTCCATGAAC CCTACAGTGA GACTGACGAG 
GATGACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330117.1
CDS	1507..8541
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQNRTPLMFQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQTVEMREMGRDGYSDTEQYLPMEGHGRAASMPRLPADNQQPRRKGRHRGENLTPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_021474442.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474442.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCTAAGGA GCAGCAGAAG AACCACAAAG GTTGTAAGTC GGTATGGGAG 
CAGCGCACTA GTGAGTTAAG ACGACAGACC CTCGTGAACA GCAGAGAGGC TCTTTATAAT 
GAGTTGGACC CTGAAGACCG TTGGAAGGTC TCATACTCCC GTCACATACG TCCCGACATG 
AAAACTCACC TGGACCGGCC GTTAGTCGTG GACCCTCAGG AGAACCGCAA TAACAACACT 
AACAAAACCC GTCCGGGTGA CGGCCAACCT CAGCATTCCC ATCAGCTCCT ACACAAACAG 
CCCAACTTCA ATGAGGCCGA GACTGGAGGA GGTGGAGGGT CTGAGTCCTG GCCCGCACTG 
GAAAGAGGAG ACTCAACAGG GTCCCGCCGC AGTCTTTTGG GCTCTGAGCA CTATGAGGAG 
GGTAGAGAGT CCCACCGCAG ACACCAGCAC AGCCAGCACT GCAGTCAGGC CCGTCAACAT 
CGCAAAGCTG CAGAGGGCGG TGGAGAGCCT GGAGGGAGGA GACACAGGAG TAAGGCCAAG 
GAAAGAGGAC CTGAGTGTGA GCATTCAGAT GGAGGGGAGC GGAAACACAG ACGGCATCGG 
CATGAGGGTG AGGGGAGGAA AGAGAGAGGG GTACGACACC GAAACAGGAG GGATGGCCAT 
GCAAGTGGCC CGACTCTCTC TACAACCCGT CCCATTCAGA AGACTCTGTC CAGGCAGGAC 
AGTCAGTACA GCGAAGACCT GGACAATGCC ATGAACAACA AACTTGCTAC CCAGCCACAT 
GATAGTCTGC TTAATCTGGC CAACACTGCC CACTCTGCCG GCCTGGCACA TACTGGTACA 
GAGAGCAGCC TCATCCTCAC CAACCCTTCC TCTACCATTG CAAATGTTAC TAGCATCGGT 
CACCTGGGTA TGAAACCTGA GTACACTGCC GTGGACATTC CTCCCATGTT CCCCTCCAGT 
AATGCCATTC TACAGGTCAA TAAGAATGCC AACACGGAGC CTCTGCCAAA GAAGGAAGAC 
ACAAAGGGTG ACGATGATGA TGATGAGAAA GATGACGGAG GGCCTAAACC CATGCCACCC 
TACACCTCAA TGTTCATTCT GACCACCACC AACCCATTTC GGAGATTGTG TCACTATATC 
GTCACACTGA GATATTTTGA GATGTGTATT CTACTGGTTA TTGCTATGAG CAGTATTGCC 
CTGGCTGCTG AAGACCCTGT CTGGCCTGAA TCTCCCCGCA ACAATGTCCT ACGATACTTT 
GACTATGTGT TCACTGGTGT GTTCACCTTT GAGATGCTCA TTAAGATGGT AGATTTAGGT 
CTTGTCCTGC ATCAAGGCTC ATACTTTCGT GATCTGTGGA ACATCCTGGA CTTTATAGTG 
GTTAGTGGTG CCCTGGTGGC GTTCGCCTTC ACAGGAAGCA GCAAAGGGAA AGACATCAGT 
ACTATCAAGT CTCTACGTGT GCTTCGAGTA CTGCGGCCGC TGAAAACCAT CAAACGTCTG 
CCAAAACTGA AGGCTGTCTT TGACTGTGTG GTGAACTCTC TGAAGAATGT GTTGAACATC 
CTCATCGTCT ACATGCTCTT CATGTTCATC TTCGCTGTGG TGGCTGTGCA GCTCTTTAAG 
GGACGTTTCT TTTACTGCAC TGATGAATCC AAAGAGTTTG AGCGCGACTG CAGGGGCGAG 
TATCTGGTCT ATGAAAGAGA TAACGAAGTG AGGTCACAGA AGCGGGAATG GAAGAAATAC 
GATTTCCACT ACGACAACGT GCTCTGGGCC CTTCTCACCC TCTTCACCGT TTCTACGGGA 
GAGGGGTGGC CGCAAGTGCT AAAACATTCA GTAGATGCGA CCTACGAGAA TCAGGGCCCG 
AGCCCAGGTT ACCGGATGGA AATGTCCATC TTTTACGTGG TGTACTTCGT GGTCTTCCCC 
TTCTTCTTCG TCAACATCTT CGTGGCTCTT ATCATCATTA CTTTCCAGGA GCAAGGAGAC 
AAAATGATGG AGGACTATAG CCTGGAAAAG AATGAGAGAG CCTGCATAGA CTTTGCCATC 
AACGCTAAGC CGCTCACACG GCACATGCCC CAGAACAAGC AGACCTTTCA GTACCGCATG 
TGGGAGTTTG TGGTGTCTCC TCCATTTGAA TACACCATCA TGGCCTTGAT CGCGCTCAAC 
ACTATTGTCC TTATGATGAA GTATGATGGA GCATCGCTTA CTTATGAAGA TGTGCTAAAA 
TACCTGAACA TAGTGTTCAC CTCTCTATTC TCCATGGAGT GCATATTGAA GATCATAGCC 
TTCGGAGCAT TGAACTACTT CAAAGATGCC TGGAACATCT TCGACTGTGT GACTGTACTT 
GGCAGCATCA CTGATATTTT AGTCACAGAG CTGGGGAACA ACTTCATAAA TCTGAGTTTT 
CTAAGGCTCT TCAGAGCTGC TCGTCTCATT AAGTTGCTCA GGCAGGGTGA AACCATACGC 
ATCCTGCTCT GGACTTTTGT CCAGTCTTTC AAGGCTCTGC CGTATGTGTG CCTACTCATC 
GCCATGCTGT TCTTCATCTA CGCCATCATT GGCATGCAGC TGTTTGGGAA CATTAAGATC 
GAGGAGAACA GTGACAGTGC CATTACCCAG CACAACAACT TCAGGACTTT TTTCCAGGCC 
CTAATGCTGC TCTTTAGGAG TGCTACAGGT GAGGCATGGC ATGACATCAT GCTGTCATGT 
CTGGGAAAGA AGCCGTGTGA TATTCTCTCT GATAACCCGA AGCCAGAATG CGGGAGCGAG 
TTTGCCTACC TCTACTTTGT CTCCTTCATC TTCCTCTGTT CATTTCTGAT GCTGAATCTG 
TTCGTGGCTG TGATCATGGA TAATTTTGAA TACCTGACGC GTGACTCCTC TATCCTGGGA 
CCCCATCACC TGGACGAGTA TGTGAGGATC TGGGCAGAAT ATGATCCTGC TGCTTGCGGG 
CGCATTCATT ATAAGGATAT GTACAGTTTA TTGCGAGTTA TCGACCCCCC TCTGGGCTTA 
GGCAAGAAAT GTCCTCATAG GGTGGCCTGC AAGAGACTGT TGCGGATGGA TCTGCCGGTT 
GCCGATGACA ACACAGTGCA CTTTAATTCT ACCCTTATGG CCTTAATCCG AACCGCTCTG 
GACATCAAGA TCGCCAAGGG AGGTGTAGAT AAGCACCAGA TGGATGCTGA ATTAAGGAAG 
GAGATGATGG CGATATGGCC TAATCTCTCT CAGAAGAACC TGGACCTGCT GGTGACTCCT 
CACAAGTCGA CAGACCTGAC AGTAGGAAAG ATCTACGCTG CCATGATGAT CATGGAGTAT 
TATCGGCAAA GTAAAGCCAA ACGGACACAA GCTCTCCATG ATGAGCAGAA TCGAACGCCA 
TTAATGTTTC AGCGTCTGGA GCCGCCATCT CCTAGCCAGG ATGTGGGACC AGGACTCACT 
GGCCTTCCTG ACACACAAAT GCACCCTGCA AATCATCTGC CTGTTGATGA AAGGATCCCA 
GAGAGCCAAT CATGGGTGAC AGCGCGAGCA CAGGAGATTT CTCAAAAAGC AGGCAACTGG 
AGTCCAGAGG GCCAACATCC AGATGACACC ACAGACAACA GAAGGCAGTC TCAGACAGTA 
GAGATGAGAG AGATGGGGCG GGACGGGTAC TCGGACACAG AGCAATATCT GCCGATGGAA 
GGCCATGGCA GGGCTGCTTC CATGCCTCGA CTCCCAGCAG ACAACCAACA GCCACGGAGG 
AAAGGGCGTC ACCGGGGAGA AAATCTCACT CCTATCACAG ATAACAGTCC TATGAAGCGC 
TCGGCTTCAT CTTTGGGACA CGGGCGTGCA GGACGCTCCA TGCGAGGTGA GGATTATGCC 
ATGGAGAGGG TTATCCCAGA AGAGGGTCAC AGGCATGGGC ATCGCCACCG AGACCGTAGT 
CACCGGGCGT CAGAACGCTC CCTGAGCCGA TACACTGATG CTGACACAGG TCTTGGAACT 
GACCTCAGCA CAACCACACA ATCAGGAGAC CTGCCATCCA AGGAGAGAGA CCGGGGCCGA 
GCCAAAGACC GCAAGCACCA TCATCATCAT CATCACCACC ACGGCTCTTT AGATAAGGAG 
CACTACGGTC ATGAGAGAGA CAGAGGAGAG TACGGCCACA GACAGTCCAG AGAGAGAGAC 
CGCCGCTGGT CCCGCTCACC CAGTGAGGGC AGAGAGTGTC TGACTCACAG ACAGGGCAGT 
AGTTCAGTAA GCGGCAGTCC AGTCCCGTCC ACCTCCGGGA CCAGCACCCC CCGCCGTGGC 
CGCCGTCAGC TCCCCCAAAC CCCTGCTACA CCCCGGCCAC ATGTCACCTA CTCACCTGTG 
GTCCGTAAGC CCATCAGCAG TACCCCCCCT CCTGGCCAGC AGAGCCGACT CCCTACCCCG 
ACCTCCCGGC GCTTCAGTCC GACTGGCCCC GAGCCCCCGC TTCCCCCTCA TCACCACCCG 
TCACCACCCC ACCACGGCTC TCCCCGCTCC GGCCGACATG CACACTGGGG CCCCGAGCCT 
GCAGAGAGCG TGGAGGGTGA CGGCTTCTAT GACGACCAAG ACTATGAGTT CAATCACCAC 
GAGCCTCCAT CCTACGAGCA AAGCCCTGTG CAGGGCAACC CTCACCCCCA TTCCCCTAGG 
ACTTCCCGCC ACAACACCCC TCCTCAGGGC CAAGCGCACC CACGGCGCAT GCCAAATGGC 
TACCGTTCGT CTTCGCCCTC TCCACACCAC CACGCGCCCG CTCACCCTGG AACTCATAAA 
CCTCCTCATC CCAGAGGGCC CAGAAAGGGC CTCCATGAAC CCTACAGTGA GACTGACGAG 
GATGACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_009299585.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297860.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X14, mRNA.

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CloneID	ODa43660
Clone ID Related Accession (Same CDS sequence)	XM_009299585.3 , XM_009299585.2
Accession Version	XM_009299585.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009299585.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG 
GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT 
GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC 
CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC 
ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT 
GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT 
GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT 
GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT 
CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA 
AGGCAGTCTC AGACAGTAGA GATGAGAGAG ATGGGGCGGG ACGGGTACTC GGACACAGAG 
CAATATCTGC CGATGGAAGG CCATGGCAGG GCTGCTTCCA TGCCTCGACT CCCAGCAGAC 
AACCAACAGC CTATCACAGA TAACAGTCCT ATGAAGCGCT CGGCTTCATC TTTGGGACAC 
GGGCGTGCAG GACGCTCCAT GCGAGGTGAG GATTATGCCA TGGAGAGGGT TATCCCAGAA 
GAGGGTCACA GGCATGGGCA TCGCCACCGA GACCGTAGTC ACCGGGCGTC AGAACGCTCC 
CTGAGCCGAT ACACTGATGC TGACACAGGT CTTGGAACTG ACCTCAGCAC AACCACACAA 
TCAGGAGACC TGCCATCCAA GGAGAGAGAC CGGGGCCGAG CCAAAGACCG CAAGCACCAT 
CATCATCATC ATCACCACCA CGGCTCTTTA GATAAGGAGC ACTACGGTCA TGAGAGAGAC 
AGAGGAGAGT ACGGCCACAG ACAGTCCAGA GAGAGAGACC GCCGCTGGTC CCGCTCACCC 
AGTGAGGGCA GAGAGTGTCT GACTCACAGA CAGGGCAGTA GTTCAGTAAG CGGCAGTCCA 
GTCCCGTCCA CCTCCGGGAC CAGCACCCCC CGCCGTGGCC GCCGTCAGCT CCCCCAAACC 
CCTGCTACAC CCCGGCCACA TGTCACCTAC TCACCTGTGG TCCGTAAGCC CATCAGCAGT 
ACCCCCCCTC CTGGCCAGCA GAGCCGACTC CCTACCCCGA CCTCCCGGCG CTTCAGTCCG 
ACTGGCCCCG AGCCCCCGCT TCCCCCTCAT CACCACCCGT CACCACCCCA CCACGGCTCT 
CCCCGCTCCG GCCGACATGC ACACTGGGGC CCCGAGCCTG CAGAGAGCGT GGAGGGTGAC 
GGCTTCTATG ACGACCAAGA CTATGAGTTC AATCACCACG AGCCTCCATC CTACGAGCAA 
AGCCCTGTGC AGGGCAACCC TCACCCCCAT TCCCCTAGGA CTTCCCGCCA CAACACCCCT 
CCTCAGGGCC AAGCGCACCC ACGGCGCATG CCAAATGGCT ACCGTTCGTC TTCGCCCTCT 
CCACACCACC ACGCGCCCGC TCACCCTGGA ACTCATAAAC CTCCTCATCC CAGAGGGCCC 
AGAAAGGGCC TCCATGAACC CTACAGTGAG ACTGACGAGG ATGACTGGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297860.1
CDS	1505..8578
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGGSGGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGMNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGEGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQNRTPLMFQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQTVEMREMGRDGYSDTEQYLPMEGHGRAASMPRLPADNQQPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_009299585.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299585.3

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG 
GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT 
GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC 
CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC 
ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT 
GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT 
GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT 
GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT 
CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA 
AGGCAGTCTC AGACAGTAGA GATGAGAGAG ATGGGGCGGG ACGGGTACTC GGACACAGAG 
CAATATCTGC CGATGGAAGG CCATGGCAGG GCTGCTTCCA TGCCTCGACT CCCAGCAGAC 
AACCAACAGC CTATCACAGA TAACAGTCCT ATGAAGCGCT CGGCTTCATC TTTGGGACAC 
GGGCGTGCAG GACGCTCCAT GCGAGGTGAG GATTATGCCA TGGAGAGGGT TATCCCAGAA 
GAGGGTCACA GGCATGGGCA TCGCCACCGA GACCGTAGTC ACCGGGCGTC AGAACGCTCC 
CTGAGCCGAT ACACTGATGC TGACACAGGT CTTGGAACTG ACCTCAGCAC AACCACACAA 
TCAGGAGACC TGCCATCCAA GGAGAGAGAC CGGGGCCGAG CCAAAGACCG CAAGCACCAT 
CATCATCATC ATCACCACCA CGGCTCTTTA GATAAGGAGC ACTACGGTCA TGAGAGAGAC 
AGAGGAGAGT ACGGCCACAG ACAGTCCAGA GAGAGAGACC GCCGCTGGTC CCGCTCACCC 
AGTGAGGGCA GAGAGTGTCT GACTCACAGA CAGGGCAGTA GTTCAGTAAG CGGCAGTCCA 
GTCCCGTCCA CCTCCGGGAC CAGCACCCCC CGCCGTGGCC GCCGTCAGCT CCCCCAAACC 
CCTGCTACAC CCCGGCCACA TGTCACCTAC TCACCTGTGG TCCGTAAGCC CATCAGCAGT 
ACCCCCCCTC CTGGCCAGCA GAGCCGACTC CCTACCCCGA CCTCCCGGCG CTTCAGTCCG 
ACTGGCCCCG AGCCCCCGCT TCCCCCTCAT CACCACCCGT CACCACCCCA CCACGGCTCT 
CCCCGCTCCG GCCGACATGC ACACTGGGGC CCCGAGCCTG CAGAGAGCGT GGAGGGTGAC 
GGCTTCTATG ACGACCAAGA CTATGAGTTC AATCACCACG AGCCTCCATC CTACGAGCAA 
AGCCCTGTGC AGGGCAACCC TCACCCCCAT TCCCCTAGGA CTTCCCGCCA CAACACCCCT 
CCTCAGGGCC AAGCGCACCC ACGGCGCATG CCAAATGGCT ACCGTTCGTC TTCGCCCTCT 
CCACACCACC ACGCGCCCGC TCACCCTGGA ACTCATAAAC CTCCTCATCC CAGAGGGCCC 
AGAAAGGGCC TCCATGAACC CTACAGTGAG ACTGACGAGG ATGACTGGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_009299585.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297860.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X1, mRNA.

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CloneID	ODa43660
Clone ID Related Accession (Same CDS sequence)	XM_009299585.3 , XM_009299585.2
Accession Version	XM_009299585.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299585.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG 
GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT 
GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC 
CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC 
ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT 
GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT 
GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT 
GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT 
CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA 
AGGCAGTCTC AGACAGTAGA GATGAGAGAG ATGGGGCGGG ACGGGTACTC GGACACAGAG 
CAATATCTGC CGATGGAAGG CCATGGCAGG GCTGCTTCCA TGCCTCGACT CCCAGCAGAC 
AACCAACAGC CTATCACAGA TAACAGTCCT ATGAAGCGCT CGGCTTCATC TTTGGGACAC 
GGGCGTGCAG GACGCTCCAT GCGAGGTGAG GATTATGCCA TGGAGAGGGT TATCCCAGAA 
GAGGGTCACA GGCATGGGCA TCGCCACCGA GACCGTAGTC ACCGGGCGTC AGAACGCTCC 
CTGAGCCGAT ACACTGATGC TGACACAGGT CTTGGAACTG ACCTCAGCAC AACCACACAA 
TCAGGAGACC TGCCATCCAA GGAGAGAGAC CGGGGCCGAG CCAAAGACCG CAAGCACCAT 
CATCATCATC ATCACCACCA CGGCTCTTTA GATAAGGAGC ACTACGGTCA TGAGAGAGAC 
AGAGGAGAGT ACGGCCACAG ACAGTCCAGA GAGAGAGACC GCCGCTGGTC CCGCTCACCC 
AGTGAGGGCA GAGAGTGTCT GACTCACAGA CAGGGCAGTA GTTCAGTAAG CGGCAGTCCA 
GTCCCGTCCA CCTCCGGGAC CAGCACCCCC CGCCGTGGCC GCCGTCAGCT CCCCCAAACC 
CCTGCTACAC CCCGGCCACA TGTCACCTAC TCACCTGTGG TCCGTAAGCC CATCAGCAGT 
ACCCCCCCTC CTGGCCAGCA GAGCCGACTC CCTACCCCGA CCTCCCGGCG CTTCAGTCCG 
ACTGGCCCCG AGCCCCCGCT TCCCCCTCAT CACCACCCGT CACCACCCCA CCACGGCTCT 
CCCCGCTCCG GCCGACATGC ACACTGGGGC CCCGAGCCTG CAGAGAGCGT GGAGGGTGAC 
GGCTTCTATG ACGACCAAGA CTATGAGTTC AATCACCACG AGCCTCCATC CTACGAGCAA 
AGCCCTGTGC AGGGCAACCC TCACCCCCAT TCCCCTAGGA CTTCCCGCCA CAACACCCCT 
CCTCAGGGCC AAGCGCACCC ACGGCGCATG CCAAATGGCT ACCGTTCGTC TTCGCCCTCT 
CCACACCACC ACGCGCCCGC TCACCCTGGA ACTCATAAAC CTCCTCATCC CAGAGGGCCC 
AGAAAGGGCC TCCATGAACC CTACAGTGAG ACTGACGAGG ATGACTGGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297860.1
CDS	1466..8539
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGGSGGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGMNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGEGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQNRTPLMFQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQTVEMREMGRDGYSDTEQYLPMEGHGRAASMPRLPADNQQPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_009299585.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299585.2

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG 
GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT 
GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC 
CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC 
ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT 
GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT 
GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT 
GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT 
CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA 
AGGCAGTCTC AGACAGTAGA GATGAGAGAG ATGGGGCGGG ACGGGTACTC GGACACAGAG 
CAATATCTGC CGATGGAAGG CCATGGCAGG GCTGCTTCCA TGCCTCGACT CCCAGCAGAC 
AACCAACAGC CTATCACAGA TAACAGTCCT ATGAAGCGCT CGGCTTCATC TTTGGGACAC 
GGGCGTGCAG GACGCTCCAT GCGAGGTGAG GATTATGCCA TGGAGAGGGT TATCCCAGAA 
GAGGGTCACA GGCATGGGCA TCGCCACCGA GACCGTAGTC ACCGGGCGTC AGAACGCTCC 
CTGAGCCGAT ACACTGATGC TGACACAGGT CTTGGAACTG ACCTCAGCAC AACCACACAA 
TCAGGAGACC TGCCATCCAA GGAGAGAGAC CGGGGCCGAG CCAAAGACCG CAAGCACCAT 
CATCATCATC ATCACCACCA CGGCTCTTTA GATAAGGAGC ACTACGGTCA TGAGAGAGAC 
AGAGGAGAGT ACGGCCACAG ACAGTCCAGA GAGAGAGACC GCCGCTGGTC CCGCTCACCC 
AGTGAGGGCA GAGAGTGTCT GACTCACAGA CAGGGCAGTA GTTCAGTAAG CGGCAGTCCA 
GTCCCGTCCA CCTCCGGGAC CAGCACCCCC CGCCGTGGCC GCCGTCAGCT CCCCCAAACC 
CCTGCTACAC CCCGGCCACA TGTCACCTAC TCACCTGTGG TCCGTAAGCC CATCAGCAGT 
ACCCCCCCTC CTGGCCAGCA GAGCCGACTC CCTACCCCGA CCTCCCGGCG CTTCAGTCCG 
ACTGGCCCCG AGCCCCCGCT TCCCCCTCAT CACCACCCGT CACCACCCCA CCACGGCTCT 
CCCCGCTCCG GCCGACATGC ACACTGGGGC CCCGAGCCTG CAGAGAGCGT GGAGGGTGAC 
GGCTTCTATG ACGACCAAGA CTATGAGTTC AATCACCACG AGCCTCCATC CTACGAGCAA 
AGCCCTGTGC AGGGCAACCC TCACCCCCAT TCCCCTAGGA CTTCCCGCCA CAACACCCCT 
CCTCAGGGCC AAGCGCACCC ACGGCGCATG CCAAATGGCT ACCGTTCGTC TTCGCCCTCT 
CCACACCACC ACGCGCCCGC TCACCCTGGA ACTCATAAAC CTCCTCATCC CAGAGGGCCC 
AGAAAGGGCC TCCATGAACC CTACAGTGAG ACTGACGAGG ATGACTGGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_009299586.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297861.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X10, mRNA.

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CloneID	ODa43661
Clone ID Related Accession (Same CDS sequence)	XM_009299586.2
Accession Version	XM_009299586.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299586.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA ACAACTTCAT AAATCTGAGT 
TTTCTAAGGC TCTTCAGAGC TGCTCGTCTC ATTAAGTTGC TCAGGCAGGG TGAAACCATA 
CGCATCCTGC TCTGGACTTT TGTCCAGTCT TTCAAGGCTC TGCCGTATGT GTGCCTACTC 
ATCGCCATGC TGTTCTTCAT CTACGCCATC ATTGGCATGC AGCTGTTTGG GAACATTAAG 
ATCGAGGAGA ACAGTGACAG TGCCATTACC CAGCACAACA ACTTCAGGAC TTTTTTCCAG 
GCCCTAATGC TGCTCTTTAG GAGTGCTACA GGTGAGGCAT GGCATGACAT CATGCTGTCA 
TGTCTGGGAA AGAAGCCGTG TGATATTCTC TCTGATAACC CGAAGCCAGA ATGCGGGAGC 
GAGTTTGCCT ACCTCTACTT TGTCTCCTTC ATCTTCCTCT GTTCATTTCT GATGCTGAAT 
CTGTTCGTGG CTGTGATCAT GGATAATTTT GAATACCTGA CGCGTGACTC CTCTATCCTG 
GGACCCCATC ACCTGGACGA GTATGTGAGG ATCTGGGCAG AATATGATCC TGCTGCTTGT 
GGCAGAATCA GTTACAGGGA TATGTATGAG ATGCTCAGGC ACATGTCTCC TCCGTTAGGC 
CTGGGGAAGA AGTGCCCTGC ACGGGTTGCC TACAAGAGAC TGTTGCGGAT GGATCTGCCG 
GTTGCCGATG ACAACACAGT GCACTTTAAT TCTACCCTTA TGGCCTTAAT CCGAACCGCT 
CTGGACATCA AGATCGCCAA GGGAGGTGTA GATAAGCACC AGATGGATGC TGAATTAAGG 
AAGGAGATGA TGGCGATATG GCCTAATCTC TCTCAGAAGA ACCTGGACCT GCTGGTGACT 
CCTCACAAGT CGACAGACCT GACAGTAGGA AAGATCTACG CTGCCATGAT GATCATGGAG 
TATTATCGGC AAAGTAAAGC CAAACGGACA CAAGCTCTCC ATGATGAGCA GAATCGAACG 
CCATTAATGT TTCAGCGTCT GGAGCCGCCA TCTCCTAGCC AGGATGTGGG ACCAGGACTC 
ACTGGCCTTC CTGACACACA AATGCACCCT GCAAATCATC TGCCTGTTGA TGAAAGGATC 
CCAGAGAGCC AATCATGGGT GACAGCGCGA GCACAGGAGA TTTCTCAAAA AGCAGGCAAC 
TGGAGTCCAG AGGGCCAACA TCCAGATGAC ACCACAGACA ACAGAAGGCA GTCTCAGACA 
GTAGAGATGA GAGAGATGGG GCGGGACGGG TACTCGGACA CAGAGCAATA TCTGCCGATG 
GAAGGCCATG GCAGGGCTGC TTCCATGCCT CGACTCCCAG CAGACAACCA ACAGCCTATC 
ACAGATAACA GTCCTATGAA GCGCTCGGCT TCATCTTTGG GACACGGGCG TGCAGGACGC 
TCCATGCGAG GTGAGGATTA TGCCATGGAG AGGGTTATCC CAGAAGAGGG TCACAGGCAT 
GGGCATCGCC ACCGAGACCG TAGTCACCGG GCGTCAGAAC GCTCCCTGAG CCGATACACT 
GATGCTGACA CAGGTCTTGG AACTGACCTC AGCACAACCA CACAATCAGG AGACCTGCCA 
TCCAAGGAGA GAGACCGGGG CCGAGCCAAA GACCGCAAGC ACCATCATCA TCATCATCAC 
CACCACGGCT CTTTAGATAA GGAGCACTAC GGTCATGAGA GAGACAGAGG AGAGTACGGC 
CACAGACAGT CCAGAGAGAG AGACCGCCGC TGGTCCCGCT CACCCAGTGA GGGCAGAGAG 
TGTCTGACTC ACAGACAGGG CAGTAGTTCA GTAAGCGGCA GTCCAGTCCC GTCCACCTCC 
GGGACCAGCA CCCCCCGCCG TGGCCGCCGT CAGCTCCCCC AAACCCCTGC TACACCCCGG 
CCACATGTCA CCTACTCACC TGTGGTCCGT AAGCCCATCA GCAGTACCCC CCCTCCTGGC 
CAGCAGAGCC GACTCCCTAC CCCGACCTCC CGGCGCTTCA GTCCGACTGG CCCCGAGCCC 
CCGCTTCCCC CTCATCACCA CCCGTCACCA CCCCACCACG GCTCTCCCCG CTCCGGCCGA 
CATGCACACT GGGGCCCCGA GCCTGCAGAG AGCGTGGAGG GTGACGGCTT CTATGACGAC 
CAAGACTATG AGTTCAATCA CCACGAGCCT CCATCCTACG AGCAAAGCCC TGTGCAGGGC 
AACCCTCACC CCCATTCCCC TAGGACTTCC CGCCACAACA CCCCTCCTCA GGGCCAAGCG 
CACCCACGGC GCATGCCAAA TGGCTACCGT TCGTCTTCGC CCTCTCCACA CCACCACGCG 
CCCGCTCACC CTGGAACTCA TAAACCTCCT CATCCCAGAG GGCCCAGAAA GGGCCTCCAT 
GAACCCTACA GTGAGACTGA CGAGGATGAC TGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297861.1
CDS	1468..8526
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGGSGGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRISYRDMYEMLRHMSPPLGLGKKCPARVAYKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQNRTPLMFQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQTVEMREMGRDGYSDTEQYLPMEGHGRAASMPRLPADNQQPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_009299586.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299586.2

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA ACAACTTCAT AAATCTGAGT 
TTTCTAAGGC TCTTCAGAGC TGCTCGTCTC ATTAAGTTGC TCAGGCAGGG TGAAACCATA 
CGCATCCTGC TCTGGACTTT TGTCCAGTCT TTCAAGGCTC TGCCGTATGT GTGCCTACTC 
ATCGCCATGC TGTTCTTCAT CTACGCCATC ATTGGCATGC AGCTGTTTGG GAACATTAAG 
ATCGAGGAGA ACAGTGACAG TGCCATTACC CAGCACAACA ACTTCAGGAC TTTTTTCCAG 
GCCCTAATGC TGCTCTTTAG GAGTGCTACA GGTGAGGCAT GGCATGACAT CATGCTGTCA 
TGTCTGGGAA AGAAGCCGTG TGATATTCTC TCTGATAACC CGAAGCCAGA ATGCGGGAGC 
GAGTTTGCCT ACCTCTACTT TGTCTCCTTC ATCTTCCTCT GTTCATTTCT GATGCTGAAT 
CTGTTCGTGG CTGTGATCAT GGATAATTTT GAATACCTGA CGCGTGACTC CTCTATCCTG 
GGACCCCATC ACCTGGACGA GTATGTGAGG ATCTGGGCAG AATATGATCC TGCTGCTTGT 
GGCAGAATCA GTTACAGGGA TATGTATGAG ATGCTCAGGC ACATGTCTCC TCCGTTAGGC 
CTGGGGAAGA AGTGCCCTGC ACGGGTTGCC TACAAGAGAC TGTTGCGGAT GGATCTGCCG 
GTTGCCGATG ACAACACAGT GCACTTTAAT TCTACCCTTA TGGCCTTAAT CCGAACCGCT 
CTGGACATCA AGATCGCCAA GGGAGGTGTA GATAAGCACC AGATGGATGC TGAATTAAGG 
AAGGAGATGA TGGCGATATG GCCTAATCTC TCTCAGAAGA ACCTGGACCT GCTGGTGACT 
CCTCACAAGT CGACAGACCT GACAGTAGGA AAGATCTACG CTGCCATGAT GATCATGGAG 
TATTATCGGC AAAGTAAAGC CAAACGGACA CAAGCTCTCC ATGATGAGCA GAATCGAACG 
CCATTAATGT TTCAGCGTCT GGAGCCGCCA TCTCCTAGCC AGGATGTGGG ACCAGGACTC 
ACTGGCCTTC CTGACACACA AATGCACCCT GCAAATCATC TGCCTGTTGA TGAAAGGATC 
CCAGAGAGCC AATCATGGGT GACAGCGCGA GCACAGGAGA TTTCTCAAAA AGCAGGCAAC 
TGGAGTCCAG AGGGCCAACA TCCAGATGAC ACCACAGACA ACAGAAGGCA GTCTCAGACA 
GTAGAGATGA GAGAGATGGG GCGGGACGGG TACTCGGACA CAGAGCAATA TCTGCCGATG 
GAAGGCCATG GCAGGGCTGC TTCCATGCCT CGACTCCCAG CAGACAACCA ACAGCCTATC 
ACAGATAACA GTCCTATGAA GCGCTCGGCT TCATCTTTGG GACACGGGCG TGCAGGACGC 
TCCATGCGAG GTGAGGATTA TGCCATGGAG AGGGTTATCC CAGAAGAGGG TCACAGGCAT 
GGGCATCGCC ACCGAGACCG TAGTCACCGG GCGTCAGAAC GCTCCCTGAG CCGATACACT 
GATGCTGACA CAGGTCTTGG AACTGACCTC AGCACAACCA CACAATCAGG AGACCTGCCA 
TCCAAGGAGA GAGACCGGGG CCGAGCCAAA GACCGCAAGC ACCATCATCA TCATCATCAC 
CACCACGGCT CTTTAGATAA GGAGCACTAC GGTCATGAGA GAGACAGAGG AGAGTACGGC 
CACAGACAGT CCAGAGAGAG AGACCGCCGC TGGTCCCGCT CACCCAGTGA GGGCAGAGAG 
TGTCTGACTC ACAGACAGGG CAGTAGTTCA GTAAGCGGCA GTCCAGTCCC GTCCACCTCC 
GGGACCAGCA CCCCCCGCCG TGGCCGCCGT CAGCTCCCCC AAACCCCTGC TACACCCCGG 
CCACATGTCA CCTACTCACC TGTGGTCCGT AAGCCCATCA GCAGTACCCC CCCTCCTGGC 
CAGCAGAGCC GACTCCCTAC CCCGACCTCC CGGCGCTTCA GTCCGACTGG CCCCGAGCCC 
CCGCTTCCCC CTCATCACCA CCCGTCACCA CCCCACCACG GCTCTCCCCG CTCCGGCCGA 
CATGCACACT GGGGCCCCGA GCCTGCAGAG AGCGTGGAGG GTGACGGCTT CTATGACGAC 
CAAGACTATG AGTTCAATCA CCACGAGCCT CCATCCTACG AGCAAAGCCC TGTGCAGGGC 
AACCCTCACC CCCATTCCCC TAGGACTTCC CGCCACAACA CCCCTCCTCA GGGCCAAGCG 
CACCCACGGC GCATGCCAAA TGGCTACCGT TCGTCTTCGC CCTCTCCACA CCACCACGCG 
CCCGCTCACC CTGGAACTCA TAAACCTCCT CATCCCAGAG GGCCCAGAAA GGGCCTCCAT 
GAACCCTACA GTGAGACTGA CGAGGATGAC TGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_009299587.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297862.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X12, mRNA.

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CloneID	ODa43662
Clone ID Related Accession (Same CDS sequence)	XM_009299587.2
Accession Version	XM_009299587.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7047bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X12
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299587.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACAGGAAGC AGCAAAGGGA AAGACATCAG TACTATCAAG 
TCTCTACGTG TGCTTCGAGT ACTGCGGCCG CTGAAAACCA TCAAACGTCT GCCAAAACTG 
AAGGCTGTCT TTGACTGTGT GGTGAACTCT CTGAAGAATG TGTTGAACAT CCTCATCGTC 
TACATGCTCT TCATGTTCAT CTTCGCTGTG GTGGCTGTGC AGCTCTTTAA GGGACGTTTC 
TTTTACTGCA CTGATGAATC CAAAGAGTTT GAGCGCGACT GCAGGGGCGA GTATCTGGTC 
TATGAAAGAG ATAACGAAGT GAGGTCACAG AAGCGGGAAT GGAAGAAATA CGATTTCCAC 
TACGACAACG TGCTCTGGGC CCTTCTCACC CTCTTCACCG TTTCTACGGG AGAGGGGTGG 
CCGCAAGTGC TAAAACATTC AGTAGATGCG ACCTACGAGA ATCAGGGCCC GAGCCCAGGT 
TACCGGATGG AAATGTCCAT CTTTTACGTG GTGTACTTCG TGGTCTTCCC CTTCTTCTTC 
GTCAACATCT TCGTGGCTCT TATCATCATT ACTTTCCAGG AGCAAGGAGA CAAAATGATG 
GAGGACTATA GCCTGGAAAA GAATGAGAGA GCCTGCATAG ACTTTGCCAT CAACGCTAAG 
CCGCTCACAC GGCACATGCC CCAGAACAAG CAGACCTTTC AGTACCGCAT GTGGGAGTTT 
GTGGTGTCTC CTCCATTTGA ATACACCATC ATGGCCTTGA TCGCGCTCAA CACTATTGTC 
CTTATGATGA AGTATGATGG AGCATCGCTT ACTTATGAAG ATGTGCTAAA ATACCTGAAC 
ATAGTGTTCA CCTCTCTATT CTCCATGGAG TGCATATTGA AGATCATAGC CTTCGGAGCA 
TTGAACTACT TCAAAGATGC CTGGAACATC TTCGACTGTG TGACTGTACT TGGCAGCATC 
ACTGATATTT TAGTCACAGA GCTGGGGAAC AACTTCATAA ATCTGAGTTT TCTAAGGCTC 
TTCAGAGCTG CTCGTCTCAT TAAGTTGCTC AGGCAGGGTG AAACCATACG CATCCTGCTC 
TGGACTTTTG TCCAGTCTTT CAAGGCTCTG CCGTATGTGT GCCTACTCAT CGCCATGCTG 
TTCTTCATCT ACGCCATCAT TGGCATGCAG CTGTTTGGGA ACATTAAGAT CGAGGAGAAC 
AGTGACAGTG CCATTACCCA GCACAACAAC TTCAGGACTT TTTTCCAGGC CCTAATGCTG 
CTCTTTAGGA GTGCTACAGG TGAGGCATGG CATGACATCA TGCTGTCATG TCTGGGAAAG 
AAGCCGTGTG ATATTCTCTC TGATAACCCG AAGCCAGAAT GCGGGAGCGA GTTTGCCTAC 
CTCTACTTTG TCTCCTTCAT CTTCCTCTGT TCATTTCTGA TGCTGAATCT GTTCGTGGCT 
GTGATCATGG ATAATTTTGA ATACCTGACG CGTGACTCCT CTATCCTGGG ACCCCATCAC 
CTGGACGAGT ATGTGAGGAT CTGGGCAGAA TATGATCCTG CTGCTTGCGG GCGCATTCAT 
TATAAGGATA TGTACAGTTT ATTGCGAGTT ATCGACCCCC CTCTGGGCTT AGGCAAGAAA 
TGTCCTCATA GGGTGGCCTG CAAGAGACTG TTGCGGATGG ATCTGCCGGT TGCCGATGAC 
AACACAGTGC ACTTTAATTC TACCCTTATG GCCTTAATCC GAACCGCTCT GGACATCAAG 
ATCGCCAAGG GAGGTGTAGA TAAGCACCAG ATGGATGCTG AATTAAGGAA GGAGATGATG 
GCGATATGGC CTAATCTCTC TCAGAAGAAC CTGGACCTGC TGGTGACTCC TCACAAGTCG 
ACAGACCTGA CAGTAGGAAA GATCTACGCT GCCATGATGA TCATGGAGTA TTATCGGCAA 
AGTAAAGCCA AACGGACACA AGCTCTCCAT GATGAGCAGA ATCGAACGCC ATTAATGTTT 
CAGCGTCTGG AGCCGCCATC TCCTAGCCAG GATGTGGGAC CAGGACTCAC TGGCCTTCCT 
GACACACAAA TGCACCCTGC AAATCATCTG CCTGTTGATG AAAGGATCCC AGAGAGCCAA 
TCATGGGTGA CAGCGCGAGC ACAGGAGATT TCTCAAAAAG CAGGCAACTG GAGTCCAGAG 
GGCCAACATC CAGATGACAC CACAGACAAC AGAAGGCAGT CTCAGACAGT AGAGATGAGA 
GAGATGGGGC GGGACGGGTA CTCGGACACA GAGCAATATC TGCCGATGGA AGGCCATGGC 
AGGGCTGCTT CCATGCCTCG ACTCCCAGCA GACAACCAAC AGCCTATCAC AGATAACAGT 
CCTATGAAGC GCTCGGCTTC ATCTTTGGGA CACGGGCGTG CAGGACGCTC CATGCGAGGT 
GAGGATTATG CCATGGAGAG GGTTATCCCA GAAGAGGGTC ACAGGCATGG GCATCGCCAC 
CGAGACCGTA GTCACCGGGC GTCAGAACGC TCCCTGAGCC GATACACTGA TGCTGACACA 
GGTCTTGGAA CTGACCTCAG CACAACCACA CAATCAGGAG ACCTGCCATC CAAGGAGAGA 
GACCGGGGCC GAGCCAAAGA CCGCAAGCAC CATCATCATC ATCATCACCA CCACGGCTCT 
TTAGATAAGG AGCACTACGG TCATGAGAGA GACAGAGGAG AGTACGGCCA CAGACAGTCC 
AGAGAGAGAG ACCGCCGCTG GTCCCGCTCA CCCAGTGAGG GCAGAGAGTG TCTGACTCAC 
AGACAGGGCA GTAGTTCAGT AAGCGGCAGT CCAGTCCCGT CCACCTCCGG GACCAGCACC 
CCCCGCCGTG GCCGCCGTCA GCTCCCCCAA ACCCCTGCTA CACCCCGGCC ACATGTCACC 
TACTCACCTG TGGTCCGTAA GCCCATCAGC AGTACCCCCC CTCCTGGCCA GCAGAGCCGA 
CTCCCTACCC CGACCTCCCG GCGCTTCAGT CCGACTGGCC CCGAGCCCCC GCTTCCCCCT 
CATCACCACC CGTCACCACC CCACCACGGC TCTCCCCGCT CCGGCCGACA TGCACACTGG 
GGCCCCGAGC CTGCAGAGAG CGTGGAGGGT GACGGCTTCT ATGACGACCA AGACTATGAG 
TTCAATCACC ACGAGCCTCC ATCCTACGAG CAAAGCCCTG TGCAGGGCAA CCCTCACCCC 
CATTCCCCTA GGACTTCCCG CCACAACACC CCTCCTCAGG GCCAAGCGCA CCCACGGCGC 
ATGCCAAATG GCTACCGTTC GTCTTCGCCC TCTCCACACC ACCACGCGCC CGCTCACCCT 
GGAACTCATA AACCTCCTCA TCCCAGAGGG CCCAGAAAGG GCCTCCATGA ACCCTACAGT 
GAGACTGACG AGGATGACTG GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297862.1
CDS	1467..8513
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQNRTPLMFQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQTVEMREMGRDGYSDTEQYLPMEGHGRAASMPRLPADNQQPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_009299587.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299587.2

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACAGGAAGC AGCAAAGGGA AAGACATCAG TACTATCAAG 
TCTCTACGTG TGCTTCGAGT ACTGCGGCCG CTGAAAACCA TCAAACGTCT GCCAAAACTG 
AAGGCTGTCT TTGACTGTGT GGTGAACTCT CTGAAGAATG TGTTGAACAT CCTCATCGTC 
TACATGCTCT TCATGTTCAT CTTCGCTGTG GTGGCTGTGC AGCTCTTTAA GGGACGTTTC 
TTTTACTGCA CTGATGAATC CAAAGAGTTT GAGCGCGACT GCAGGGGCGA GTATCTGGTC 
TATGAAAGAG ATAACGAAGT GAGGTCACAG AAGCGGGAAT GGAAGAAATA CGATTTCCAC 
TACGACAACG TGCTCTGGGC CCTTCTCACC CTCTTCACCG TTTCTACGGG AGAGGGGTGG 
CCGCAAGTGC TAAAACATTC AGTAGATGCG ACCTACGAGA ATCAGGGCCC GAGCCCAGGT 
TACCGGATGG AAATGTCCAT CTTTTACGTG GTGTACTTCG TGGTCTTCCC CTTCTTCTTC 
GTCAACATCT TCGTGGCTCT TATCATCATT ACTTTCCAGG AGCAAGGAGA CAAAATGATG 
GAGGACTATA GCCTGGAAAA GAATGAGAGA GCCTGCATAG ACTTTGCCAT CAACGCTAAG 
CCGCTCACAC GGCACATGCC CCAGAACAAG CAGACCTTTC AGTACCGCAT GTGGGAGTTT 
GTGGTGTCTC CTCCATTTGA ATACACCATC ATGGCCTTGA TCGCGCTCAA CACTATTGTC 
CTTATGATGA AGTATGATGG AGCATCGCTT ACTTATGAAG ATGTGCTAAA ATACCTGAAC 
ATAGTGTTCA CCTCTCTATT CTCCATGGAG TGCATATTGA AGATCATAGC CTTCGGAGCA 
TTGAACTACT TCAAAGATGC CTGGAACATC TTCGACTGTG TGACTGTACT TGGCAGCATC 
ACTGATATTT TAGTCACAGA GCTGGGGAAC AACTTCATAA ATCTGAGTTT TCTAAGGCTC 
TTCAGAGCTG CTCGTCTCAT TAAGTTGCTC AGGCAGGGTG AAACCATACG CATCCTGCTC 
TGGACTTTTG TCCAGTCTTT CAAGGCTCTG CCGTATGTGT GCCTACTCAT CGCCATGCTG 
TTCTTCATCT ACGCCATCAT TGGCATGCAG CTGTTTGGGA ACATTAAGAT CGAGGAGAAC 
AGTGACAGTG CCATTACCCA GCACAACAAC TTCAGGACTT TTTTCCAGGC CCTAATGCTG 
CTCTTTAGGA GTGCTACAGG TGAGGCATGG CATGACATCA TGCTGTCATG TCTGGGAAAG 
AAGCCGTGTG ATATTCTCTC TGATAACCCG AAGCCAGAAT GCGGGAGCGA GTTTGCCTAC 
CTCTACTTTG TCTCCTTCAT CTTCCTCTGT TCATTTCTGA TGCTGAATCT GTTCGTGGCT 
GTGATCATGG ATAATTTTGA ATACCTGACG CGTGACTCCT CTATCCTGGG ACCCCATCAC 
CTGGACGAGT ATGTGAGGAT CTGGGCAGAA TATGATCCTG CTGCTTGCGG GCGCATTCAT 
TATAAGGATA TGTACAGTTT ATTGCGAGTT ATCGACCCCC CTCTGGGCTT AGGCAAGAAA 
TGTCCTCATA GGGTGGCCTG CAAGAGACTG TTGCGGATGG ATCTGCCGGT TGCCGATGAC 
AACACAGTGC ACTTTAATTC TACCCTTATG GCCTTAATCC GAACCGCTCT GGACATCAAG 
ATCGCCAAGG GAGGTGTAGA TAAGCACCAG ATGGATGCTG AATTAAGGAA GGAGATGATG 
GCGATATGGC CTAATCTCTC TCAGAAGAAC CTGGACCTGC TGGTGACTCC TCACAAGTCG 
ACAGACCTGA CAGTAGGAAA GATCTACGCT GCCATGATGA TCATGGAGTA TTATCGGCAA 
AGTAAAGCCA AACGGACACA AGCTCTCCAT GATGAGCAGA ATCGAACGCC ATTAATGTTT 
CAGCGTCTGG AGCCGCCATC TCCTAGCCAG GATGTGGGAC CAGGACTCAC TGGCCTTCCT 
GACACACAAA TGCACCCTGC AAATCATCTG CCTGTTGATG AAAGGATCCC AGAGAGCCAA 
TCATGGGTGA CAGCGCGAGC ACAGGAGATT TCTCAAAAAG CAGGCAACTG GAGTCCAGAG 
GGCCAACATC CAGATGACAC CACAGACAAC AGAAGGCAGT CTCAGACAGT AGAGATGAGA 
GAGATGGGGC GGGACGGGTA CTCGGACACA GAGCAATATC TGCCGATGGA AGGCCATGGC 
AGGGCTGCTT CCATGCCTCG ACTCCCAGCA GACAACCAAC AGCCTATCAC AGATAACAGT 
CCTATGAAGC GCTCGGCTTC ATCTTTGGGA CACGGGCGTG CAGGACGCTC CATGCGAGGT 
GAGGATTATG CCATGGAGAG GGTTATCCCA GAAGAGGGTC ACAGGCATGG GCATCGCCAC 
CGAGACCGTA GTCACCGGGC GTCAGAACGC TCCCTGAGCC GATACACTGA TGCTGACACA 
GGTCTTGGAA CTGACCTCAG CACAACCACA CAATCAGGAG ACCTGCCATC CAAGGAGAGA 
GACCGGGGCC GAGCCAAAGA CCGCAAGCAC CATCATCATC ATCATCACCA CCACGGCTCT 
TTAGATAAGG AGCACTACGG TCATGAGAGA GACAGAGGAG AGTACGGCCA CAGACAGTCC 
AGAGAGAGAG ACCGCCGCTG GTCCCGCTCA CCCAGTGAGG GCAGAGAGTG TCTGACTCAC 
AGACAGGGCA GTAGTTCAGT AAGCGGCAGT CCAGTCCCGT CCACCTCCGG GACCAGCACC 
CCCCGCCGTG GCCGCCGTCA GCTCCCCCAA ACCCCTGCTA CACCCCGGCC ACATGTCACC 
TACTCACCTG TGGTCCGTAA GCCCATCAGC AGTACCCCCC CTCCTGGCCA GCAGAGCCGA 
CTCCCTACCC CGACCTCCCG GCGCTTCAGT CCGACTGGCC CCGAGCCCCC GCTTCCCCCT 
CATCACCACC CGTCACCACC CCACCACGGC TCTCCCCGCT CCGGCCGACA TGCACACTGG 
GGCCCCGAGC CTGCAGAGAG CGTGGAGGGT GACGGCTTCT ATGACGACCA AGACTATGAG 
TTCAATCACC ACGAGCCTCC ATCCTACGAG CAAAGCCCTG TGCAGGGCAA CCCTCACCCC 
CATTCCCCTA GGACTTCCCG CCACAACACC CCTCCTCAGG GCCAAGCGCA CCCACGGCGC 
ATGCCAAATG GCTACCGTTC GTCTTCGCCC TCTCCACACC ACCACGCGCC CGCTCACCCT 
GGAACTCATA AACCTCCTCA TCCCAGAGGG CCCAGAAAGG GCCTCCATGA ACCCTACAGT 
GAGACTGACG AGGATGACTG GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_009299588.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297863.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X13, mRNA.

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CloneID	ODa43663
Clone ID Related Accession (Same CDS sequence)	XM_009299588.2
Accession Version	XM_009299588.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7047bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X13
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299588.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACAGGAAGC AGCAAAGGGA AAGACATCAG TACTATCAAG 
TCTCTACGTG TGCTTCGAGT ACTGCGGCCG CTGAAAACCA TCAAACGTCT GCCAAAACTG 
AAGGCTGTCT TTGACTGTGT GGTGAACTCT CTGAAGAATG TGTTGAACAT CCTCATCGTC 
TACATGCTCT TCATGTTCAT CTTCGCTGTG GTGGCTGTGC AGCTCTTTAA GGGACGTTTC 
TTTTACTGCA CTGATGAATC CAAAGAGTTT GAGCGCGACT GCAGGGGCGA GTATCTGGTC 
TATGAAAGAG ATAACGAAGT GAGGTCACAG AAGCGGGAAT GGAAGAAATA CGATTTCCAC 
TACGACAACG TGCTCTGGGC CCTTCTCACC CTCTTCACCG TTTCTACGGG AGAGGGGTGG 
CCGCAAGTGC TAAAACATTC AGTAGATGCG ACCTACGAGA ATCAGGGCCC GAGCCCAGGT 
TACCGGATGG AAATGTCCAT CTTTTACGTG GTGTACTTCG TGGTCTTCCC CTTCTTCTTC 
GTCAACATCT TCGTGGCTCT TATCATCATT ACTTTCCAGG AGCAAGGAGA CAAAATGATG 
GAGGACTATA GCCTGGAAAA GAATGAGAGA GCCTGCATAG ACTTTGCCAT CAACGCTAAG 
CCGCTCACAC GGCACATGCC CCAGAACAAG CAGACCTTTC AGTACCGCAT GTGGGAGTTT 
GTGGTGTCTC CTCCATTTGA ATACACCATC ATGGCCTTGA TCGCGCTCAA CACTATTGTC 
CTTATGATGA AGTATGATGG AGCATCGCTT ACTTATGAAG ATGTGCTAAA ATACCTGAAC 
ATAGTGTTCA CCTCTCTATT CTCCATGGAG TGCATATTGA AGATCATAGC CTTCGGAGCA 
TTGAACTACT TCAAAGATGC CTGGAACATC TTCGACTGTG TGACTGTACT TGGCAGCATC 
ACTGATATTT TAGTCACAGA GCTGGGGAAC AACTTCATAA ATCTGAGTTT TCTAAGGCTC 
TTCAGAGCTG CTCGTCTCAT TAAGTTGCTC AGGCAGGGTG AAACCATACG CATCCTGCTC 
TGGACTTTTG TCCAGTCTTT CAAGGCTCTG CCGTATGTGT GCCTACTCAT CGCCATGCTG 
TTCTTCATCT ACGCCATCAT TGGCATGCAG CTGTTTGGGA ACATTAAGAT CGAGGAGAAC 
AGTGACAGTG CCATTACCCA GCACAACAAC TTCAGGACTT TTTTCCAGGC CCTAATGCTG 
CTCTTTAGGA GTGCTACAGG TGAGGCATGG CATGACATCA TGCTGTCATG TCTGGGAAAG 
AAGCCGTGTG ATATTCTCTC TGATAACCCG AAGCCAGAAT GCGGGAGCGA GTTTGCCTAC 
CTCTACTTTG TCTCCTTCAT CTTCCTCTGT TCATTTCTGA TGCTGAATCT GTTCGTGGCT 
GTGATCATGG ATAATTTTGA ATACCTGACG CGTGACTCCT CTATCCTGGG ACCCCATCAC 
CTGGACGAGT ATGTGAGGAT CTGGGCAGAA TATGATCCTG CTGCTTGTGG CAGAATCAGT 
TACAGGGATA TGTATGAGAT GCTCAGGCAC ATGTCTCCTC CGTTAGGCCT GGGGAAGAAG 
TGCCCTGCAC GGGTTGCCTA CAAGAGACTG TTGCGGATGG ATCTGCCGGT TGCCGATGAC 
AACACAGTGC ACTTTAATTC TACCCTTATG GCCTTAATCC GAACCGCTCT GGACATCAAG 
ATCGCCAAGG GAGGTGTAGA TAAGCACCAG ATGGATGCTG AATTAAGGAA GGAGATGATG 
GCGATATGGC CTAATCTCTC TCAGAAGAAC CTGGACCTGC TGGTGACTCC TCACAAGTCG 
ACAGACCTGA CAGTAGGAAA GATCTACGCT GCCATGATGA TCATGGAGTA TTATCGGCAA 
AGTAAAGCCA AACGGACACA AGCTCTCCAT GATGAGCAGA ATCGAACGCC ATTAATGTTT 
CAGCGTCTGG AGCCGCCATC TCCTAGCCAG GATGTGGGAC CAGGACTCAC TGGCCTTCCT 
GACACACAAA TGCACCCTGC AAATCATCTG CCTGTTGATG AAAGGATCCC AGAGAGCCAA 
TCATGGGTGA CAGCGCGAGC ACAGGAGATT TCTCAAAAAG CAGGCAACTG GAGTCCAGAG 
GGCCAACATC CAGATGACAC CACAGACAAC AGAAGGCAGT CTCAGACAGT AGAGATGAGA 
GAGATGGGGC GGGACGGGTA CTCGGACACA GAGCAATATC TGCCGATGGA AGGCCATGGC 
AGGGCTGCTT CCATGCCTCG ACTCCCAGCA GACAACCAAC AGCCTATCAC AGATAACAGT 
CCTATGAAGC GCTCGGCTTC ATCTTTGGGA CACGGGCGTG CAGGACGCTC CATGCGAGGT 
GAGGATTATG CCATGGAGAG GGTTATCCCA GAAGAGGGTC ACAGGCATGG GCATCGCCAC 
CGAGACCGTA GTCACCGGGC GTCAGAACGC TCCCTGAGCC GATACACTGA TGCTGACACA 
GGTCTTGGAA CTGACCTCAG CACAACCACA CAATCAGGAG ACCTGCCATC CAAGGAGAGA 
GACCGGGGCC GAGCCAAAGA CCGCAAGCAC CATCATCATC ATCATCACCA CCACGGCTCT 
TTAGATAAGG AGCACTACGG TCATGAGAGA GACAGAGGAG AGTACGGCCA CAGACAGTCC 
AGAGAGAGAG ACCGCCGCTG GTCCCGCTCA CCCAGTGAGG GCAGAGAGTG TCTGACTCAC 
AGACAGGGCA GTAGTTCAGT AAGCGGCAGT CCAGTCCCGT CCACCTCCGG GACCAGCACC 
CCCCGCCGTG GCCGCCGTCA GCTCCCCCAA ACCCCTGCTA CACCCCGGCC ACATGTCACC 
TACTCACCTG TGGTCCGTAA GCCCATCAGC AGTACCCCCC CTCCTGGCCA GCAGAGCCGA 
CTCCCTACCC CGACCTCCCG GCGCTTCAGT CCGACTGGCC CCGAGCCCCC GCTTCCCCCT 
CATCACCACC CGTCACCACC CCACCACGGC TCTCCCCGCT CCGGCCGACA TGCACACTGG 
GGCCCCGAGC CTGCAGAGAG CGTGGAGGGT GACGGCTTCT ATGACGACCA AGACTATGAG 
TTCAATCACC ACGAGCCTCC ATCCTACGAG CAAAGCCCTG TGCAGGGCAA CCCTCACCCC 
CATTCCCCTA GGACTTCCCG CCACAACACC CCTCCTCAGG GCCAAGCGCA CCCACGGCGC 
ATGCCAAATG GCTACCGTTC GTCTTCGCCC TCTCCACACC ACCACGCGCC CGCTCACCCT 
GGAACTCATA AACCTCCTCA TCCCAGAGGG CCCAGAAAGG GCCTCCATGA ACCCTACAGT 
GAGACTGACG AGGATGACTG GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297863.1
CDS	1467..8513
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRISYRDMYEMLRHMSPPLGLGKKCPARVAYKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQNRTPLMFQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQTVEMREMGRDGYSDTEQYLPMEGHGRAASMPRLPADNQQPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_009299588.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299588.2

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACAGGAAGC AGCAAAGGGA AAGACATCAG TACTATCAAG 
TCTCTACGTG TGCTTCGAGT ACTGCGGCCG CTGAAAACCA TCAAACGTCT GCCAAAACTG 
AAGGCTGTCT TTGACTGTGT GGTGAACTCT CTGAAGAATG TGTTGAACAT CCTCATCGTC 
TACATGCTCT TCATGTTCAT CTTCGCTGTG GTGGCTGTGC AGCTCTTTAA GGGACGTTTC 
TTTTACTGCA CTGATGAATC CAAAGAGTTT GAGCGCGACT GCAGGGGCGA GTATCTGGTC 
TATGAAAGAG ATAACGAAGT GAGGTCACAG AAGCGGGAAT GGAAGAAATA CGATTTCCAC 
TACGACAACG TGCTCTGGGC CCTTCTCACC CTCTTCACCG TTTCTACGGG AGAGGGGTGG 
CCGCAAGTGC TAAAACATTC AGTAGATGCG ACCTACGAGA ATCAGGGCCC GAGCCCAGGT 
TACCGGATGG AAATGTCCAT CTTTTACGTG GTGTACTTCG TGGTCTTCCC CTTCTTCTTC 
GTCAACATCT TCGTGGCTCT TATCATCATT ACTTTCCAGG AGCAAGGAGA CAAAATGATG 
GAGGACTATA GCCTGGAAAA GAATGAGAGA GCCTGCATAG ACTTTGCCAT CAACGCTAAG 
CCGCTCACAC GGCACATGCC CCAGAACAAG CAGACCTTTC AGTACCGCAT GTGGGAGTTT 
GTGGTGTCTC CTCCATTTGA ATACACCATC ATGGCCTTGA TCGCGCTCAA CACTATTGTC 
CTTATGATGA AGTATGATGG AGCATCGCTT ACTTATGAAG ATGTGCTAAA ATACCTGAAC 
ATAGTGTTCA CCTCTCTATT CTCCATGGAG TGCATATTGA AGATCATAGC CTTCGGAGCA 
TTGAACTACT TCAAAGATGC CTGGAACATC TTCGACTGTG TGACTGTACT TGGCAGCATC 
ACTGATATTT TAGTCACAGA GCTGGGGAAC AACTTCATAA ATCTGAGTTT TCTAAGGCTC 
TTCAGAGCTG CTCGTCTCAT TAAGTTGCTC AGGCAGGGTG AAACCATACG CATCCTGCTC 
TGGACTTTTG TCCAGTCTTT CAAGGCTCTG CCGTATGTGT GCCTACTCAT CGCCATGCTG 
TTCTTCATCT ACGCCATCAT TGGCATGCAG CTGTTTGGGA ACATTAAGAT CGAGGAGAAC 
AGTGACAGTG CCATTACCCA GCACAACAAC TTCAGGACTT TTTTCCAGGC CCTAATGCTG 
CTCTTTAGGA GTGCTACAGG TGAGGCATGG CATGACATCA TGCTGTCATG TCTGGGAAAG 
AAGCCGTGTG ATATTCTCTC TGATAACCCG AAGCCAGAAT GCGGGAGCGA GTTTGCCTAC 
CTCTACTTTG TCTCCTTCAT CTTCCTCTGT TCATTTCTGA TGCTGAATCT GTTCGTGGCT 
GTGATCATGG ATAATTTTGA ATACCTGACG CGTGACTCCT CTATCCTGGG ACCCCATCAC 
CTGGACGAGT ATGTGAGGAT CTGGGCAGAA TATGATCCTG CTGCTTGTGG CAGAATCAGT 
TACAGGGATA TGTATGAGAT GCTCAGGCAC ATGTCTCCTC CGTTAGGCCT GGGGAAGAAG 
TGCCCTGCAC GGGTTGCCTA CAAGAGACTG TTGCGGATGG ATCTGCCGGT TGCCGATGAC 
AACACAGTGC ACTTTAATTC TACCCTTATG GCCTTAATCC GAACCGCTCT GGACATCAAG 
ATCGCCAAGG GAGGTGTAGA TAAGCACCAG ATGGATGCTG AATTAAGGAA GGAGATGATG 
GCGATATGGC CTAATCTCTC TCAGAAGAAC CTGGACCTGC TGGTGACTCC TCACAAGTCG 
ACAGACCTGA CAGTAGGAAA GATCTACGCT GCCATGATGA TCATGGAGTA TTATCGGCAA 
AGTAAAGCCA AACGGACACA AGCTCTCCAT GATGAGCAGA ATCGAACGCC ATTAATGTTT 
CAGCGTCTGG AGCCGCCATC TCCTAGCCAG GATGTGGGAC CAGGACTCAC TGGCCTTCCT 
GACACACAAA TGCACCCTGC AAATCATCTG CCTGTTGATG AAAGGATCCC AGAGAGCCAA 
TCATGGGTGA CAGCGCGAGC ACAGGAGATT TCTCAAAAAG CAGGCAACTG GAGTCCAGAG 
GGCCAACATC CAGATGACAC CACAGACAAC AGAAGGCAGT CTCAGACAGT AGAGATGAGA 
GAGATGGGGC GGGACGGGTA CTCGGACACA GAGCAATATC TGCCGATGGA AGGCCATGGC 
AGGGCTGCTT CCATGCCTCG ACTCCCAGCA GACAACCAAC AGCCTATCAC AGATAACAGT 
CCTATGAAGC GCTCGGCTTC ATCTTTGGGA CACGGGCGTG CAGGACGCTC CATGCGAGGT 
GAGGATTATG CCATGGAGAG GGTTATCCCA GAAGAGGGTC ACAGGCATGG GCATCGCCAC 
CGAGACCGTA GTCACCGGGC GTCAGAACGC TCCCTGAGCC GATACACTGA TGCTGACACA 
GGTCTTGGAA CTGACCTCAG CACAACCACA CAATCAGGAG ACCTGCCATC CAAGGAGAGA 
GACCGGGGCC GAGCCAAAGA CCGCAAGCAC CATCATCATC ATCATCACCA CCACGGCTCT 
TTAGATAAGG AGCACTACGG TCATGAGAGA GACAGAGGAG AGTACGGCCA CAGACAGTCC 
AGAGAGAGAG ACCGCCGCTG GTCCCGCTCA CCCAGTGAGG GCAGAGAGTG TCTGACTCAC 
AGACAGGGCA GTAGTTCAGT AAGCGGCAGT CCAGTCCCGT CCACCTCCGG GACCAGCACC 
CCCCGCCGTG GCCGCCGTCA GCTCCCCCAA ACCCCTGCTA CACCCCGGCC ACATGTCACC 
TACTCACCTG TGGTCCGTAA GCCCATCAGC AGTACCCCCC CTCCTGGCCA GCAGAGCCGA 
CTCCCTACCC CGACCTCCCG GCGCTTCAGT CCGACTGGCC CCGAGCCCCC GCTTCCCCCT 
CATCACCACC CGTCACCACC CCACCACGGC TCTCCCCGCT CCGGCCGACA TGCACACTGG 
GGCCCCGAGC CTGCAGAGAG CGTGGAGGGT GACGGCTTCT ATGACGACCA AGACTATGAG 
TTCAATCACC ACGAGCCTCC ATCCTACGAG CAAAGCCCTG TGCAGGGCAA CCCTCACCCC 
CATTCCCCTA GGACTTCCCG CCACAACACC CCTCCTCAGG GCCAAGCGCA CCCACGGCGC 
ATGCCAAATG GCTACCGTTC GTCTTCGCCC TCTCCACACC ACCACGCGCC CGCTCACCCT 
GGAACTCATA AACCTCCTCA TCCCAGAGGG CCCAGAAAGG GCCTCCATGA ACCCTACAGT 
GAGACTGACG AGGATGACTG GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_009299589.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297864.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X14, mRNA.

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CloneID	ODa43664
Clone ID Related Accession (Same CDS sequence)	XM_009299589.2
Accession Version	XM_009299589.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7038bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X14
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299589.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG 
GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGAGGT GTAGATAAGC ACCAGATGGA TGCTGAATTA 
AGGAAGGAGA TGATGGCGAT ATGGCCTAAT CTCTCTCAGA AGAACCTGGA CCTGCTGGTG 
ACTCCTCACA AGTCGACAGA CCTGACAGTA GGAAAGATCT ACGCTGCCAT GATGATCATG 
GAGTATTATC GGCAAAGTAA AGCCAAACGG ACACAAGCTC TCCATGATGA GCAGCGTCTG 
GAGCCGCCAT CTCCTAGCCA GGATGTGGGA CCAGGACTCA CTGGCCTTCC TGACACACAA 
ATGCACCCTG CAAATCATCT GCCTGTTGAT GAAAGGATCC CAGAGAGCCA ATCATGGGTG 
ACAGCGCGAG CACAGGAGAT TTCTCAAAAA GCAGGCAACT GGAGTCCAGA GGGCCAACAT 
CCAGATGACA CCACAGACAA CAGAAGGCAG TCTCAGACAG TAGAGATGAG AGAGATGGGG 
CGGGACGGGT ACTCGGACAC AGAGCAATAT CTGCCGATGG AAGGCCATGG CAGGGCTGCT 
TCCATGCCTC GACTCCCAGC AGACAACCAA CAGCCTATCA CAGATAACAG TCCTATGAAG 
CGCTCGGCTT CATCTTTGGG ACACGGGCGT GCAGGACGCT CCATGCGAGG TGAGGATTAT 
GCCATGGAGA GGGTTATCCC AGAAGAGGGT CACAGGCATG GGCATCGCCA CCGAGACCGT 
AGTCACCGGG CGTCAGAACG CTCCCTGAGC CGATACACTG ATGCTGACAC AGGTCTTGGA 
ACTGACCTCA GCACAACCAC ACAATCAGGA GACCTGCCAT CCAAGGAGAG AGACCGGGGC 
CGAGCCAAAG ACCGCAAGCA CCATCATCAT CATCATCACC ACCACGGCTC TTTAGATAAG 
GAGCACTACG GTCATGAGAG AGACAGAGGA GAGTACGGCC ACAGACAGTC CAGAGAGAGA 
GACCGCCGCT GGTCCCGCTC ACCCAGTGAG GGCAGAGAGT GTCTGACTCA CAGACAGGGC 
AGTAGTTCAG TAAGCGGCAG TCCAGTCCCG TCCACCTCCG GGACCAGCAC CCCCCGCCGT 
GGCCGCCGTC AGCTCCCCCA AACCCCTGCT ACACCCCGGC CACATGTCAC CTACTCACCT 
GTGGTCCGTA AGCCCATCAG CAGTACCCCC CCTCCTGGCC AGCAGAGCCG ACTCCCTACC 
CCGACCTCCC GGCGCTTCAG TCCGACTGGC CCCGAGCCCC CGCTTCCCCC TCATCACCAC 
CCGTCACCAC CCCACCACGG CTCTCCCCGC TCCGGCCGAC ATGCACACTG GGGCCCCGAG 
CCTGCAGAGA GCGTGGAGGG TGACGGCTTC TATGACGACC AAGACTATGA GTTCAATCAC 
CACGAGCCTC CATCCTACGA GCAAAGCCCT GTGCAGGGCA ACCCTCACCC CCATTCCCCT 
AGGACTTCCC GCCACAACAC CCCTCCTCAG GGCCAAGCGC ACCCACGGCG CATGCCAAAT 
GGCTACCGTT CGTCTTCGCC CTCTCCACAC CACCACGCGC CCGCTCACCC TGGAACTCAT 
AAACCTCCTC ATCCCAGAGG GCCCAGAAAG GGCCTCCATG AACCCTACAG TGAGACTGAC 
GAGGATGACT GGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297864.1
CDS	1469..8506
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGGSGGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGMNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQTVEMREMGRDGYSDTEQYLPMEGHGRAASMPRLPADNQQPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_009299589.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299589.2

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG 
GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGAGGT GTAGATAAGC ACCAGATGGA TGCTGAATTA 
AGGAAGGAGA TGATGGCGAT ATGGCCTAAT CTCTCTCAGA AGAACCTGGA CCTGCTGGTG 
ACTCCTCACA AGTCGACAGA CCTGACAGTA GGAAAGATCT ACGCTGCCAT GATGATCATG 
GAGTATTATC GGCAAAGTAA AGCCAAACGG ACACAAGCTC TCCATGATGA GCAGCGTCTG 
GAGCCGCCAT CTCCTAGCCA GGATGTGGGA CCAGGACTCA CTGGCCTTCC TGACACACAA 
ATGCACCCTG CAAATCATCT GCCTGTTGAT GAAAGGATCC CAGAGAGCCA ATCATGGGTG 
ACAGCGCGAG CACAGGAGAT TTCTCAAAAA GCAGGCAACT GGAGTCCAGA GGGCCAACAT 
CCAGATGACA CCACAGACAA CAGAAGGCAG TCTCAGACAG TAGAGATGAG AGAGATGGGG 
CGGGACGGGT ACTCGGACAC AGAGCAATAT CTGCCGATGG AAGGCCATGG CAGGGCTGCT 
TCCATGCCTC GACTCCCAGC AGACAACCAA CAGCCTATCA CAGATAACAG TCCTATGAAG 
CGCTCGGCTT CATCTTTGGG ACACGGGCGT GCAGGACGCT CCATGCGAGG TGAGGATTAT 
GCCATGGAGA GGGTTATCCC AGAAGAGGGT CACAGGCATG GGCATCGCCA CCGAGACCGT 
AGTCACCGGG CGTCAGAACG CTCCCTGAGC CGATACACTG ATGCTGACAC AGGTCTTGGA 
ACTGACCTCA GCACAACCAC ACAATCAGGA GACCTGCCAT CCAAGGAGAG AGACCGGGGC 
CGAGCCAAAG ACCGCAAGCA CCATCATCAT CATCATCACC ACCACGGCTC TTTAGATAAG 
GAGCACTACG GTCATGAGAG AGACAGAGGA GAGTACGGCC ACAGACAGTC CAGAGAGAGA 
GACCGCCGCT GGTCCCGCTC ACCCAGTGAG GGCAGAGAGT GTCTGACTCA CAGACAGGGC 
AGTAGTTCAG TAAGCGGCAG TCCAGTCCCG TCCACCTCCG GGACCAGCAC CCCCCGCCGT 
GGCCGCCGTC AGCTCCCCCA AACCCCTGCT ACACCCCGGC CACATGTCAC CTACTCACCT 
GTGGTCCGTA AGCCCATCAG CAGTACCCCC CCTCCTGGCC AGCAGAGCCG ACTCCCTACC 
CCGACCTCCC GGCGCTTCAG TCCGACTGGC CCCGAGCCCC CGCTTCCCCC TCATCACCAC 
CCGTCACCAC CCCACCACGG CTCTCCCCGC TCCGGCCGAC ATGCACACTG GGGCCCCGAG 
CCTGCAGAGA GCGTGGAGGG TGACGGCTTC TATGACGACC AAGACTATGA GTTCAATCAC 
CACGAGCCTC CATCCTACGA GCAAAGCCCT GTGCAGGGCA ACCCTCACCC CCATTCCCCT 
AGGACTTCCC GCCACAACAC CCCTCCTCAG GGCCAAGCGC ACCCACGGCG CATGCCAAAT 
GGCTACCGTT CGTCTTCGCC CTCTCCACAC CACCACGCGC CCGCTCACCC TGGAACTCAT 
AAACCTCCTC ATCCCAGAGG GCCCAGAAAG GGCCTCCATG AACCCTACAG TGAGACTGAC 
GAGGATGACT GGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_009299590.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297865.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X20, mRNA.

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CloneID	ODa43665
Clone ID Related Accession (Same CDS sequence)	XM_009299590.3 , XM_009299590.2
Accession Version	XM_009299590.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009299590.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG 
GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT 
GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC 
CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC 
ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT 
GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT 
GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT 
GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT 
CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA 
AGGCAGTCTC AGCCTATCAC AGATAACAGT CCTATGAAGC GCTCGGCTTC ATCTTTGGGA 
CACGGGCGTG CAGGACGCTC CATGCGAGGT GAGGATTATG CCATGGAGAG GGTTATCCCA 
GAAGAGGGTC ACAGGCATGG GCATCGCCAC CGAGACCGTA GTCACCGGGC GTCAGAACGC 
TCCCTGAGCC GATACACTGA TGCTGACACA GGTCTTGGAA CTGACCTCAG CACAACCACA 
CAATCAGGAG ACCTGCCATC CAAGGAGAGA GACCGGGGCC GAGCCAAAGA CCGCAAGCAC 
CATCATCATC ATCATCACCA CCACGGCTCT TTAGATAAGG AGCACTACGG TCATGAGAGA 
GACAGAGGAG AGTACGGCCA CAGACAGTCC AGAGAGAGAG ACCGCCGCTG GTCCCGCTCA 
CCCAGTGAGG GCAGAGAGTG TCTGACTCAC AGACAGGGCA GTAGTTCAGT AAGCGGCAGT 
CCAGTCCCGT CCACCTCCGG GACCAGCACC CCCCGCCGTG GCCGCCGTCA GCTCCCCCAA 
ACCCCTGCTA CACCCCGGCC ACATGTCACC TACTCACCTG TGGTCCGTAA GCCCATCAGC 
AGTACCCCCC CTCCTGGCCA GCAGAGCCGA CTCCCTACCC CGACCTCCCG GCGCTTCAGT 
CCGACTGGCC CCGAGCCCCC GCTTCCCCCT CATCACCACC CGTCACCACC CCACCACGGC 
TCTCCCCGCT CCGGCCGACA TGCACACTGG GGCCCCGAGC CTGCAGAGAG CGTGGAGGGT 
GACGGCTTCT ATGACGACCA AGACTATGAG TTCAATCACC ACGAGCCTCC ATCCTACGAG 
CAAAGCCCTG TGCAGGGCAA CCCTCACCCC CATTCCCCTA GGACTTCCCG CCACAACACC 
CCTCCTCAGG GCCAAGCGCA CCCACGGCGC ATGCCAAATG GCTACCGTTC GTCTTCGCCC 
TCTCCACACC ACCACGCGCC CGCTCACCCT GGAACTCATA AACCTCCTCA TCCCAGAGGG 
CCCAGAAAGG GCCTCCATGA ACCCTACAGT GAGACTGACG AGGATGACTG GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297865.1
CDS	1508..8464
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGGSGGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGMNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGEGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQNRTPLMFQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_009299590.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299590.3

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG 
GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT 
GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC 
CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC 
ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT 
GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT 
GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT 
GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT 
CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA 
AGGCAGTCTC AGCCTATCAC AGATAACAGT CCTATGAAGC GCTCGGCTTC ATCTTTGGGA 
CACGGGCGTG CAGGACGCTC CATGCGAGGT GAGGATTATG CCATGGAGAG GGTTATCCCA 
GAAGAGGGTC ACAGGCATGG GCATCGCCAC CGAGACCGTA GTCACCGGGC GTCAGAACGC 
TCCCTGAGCC GATACACTGA TGCTGACACA GGTCTTGGAA CTGACCTCAG CACAACCACA 
CAATCAGGAG ACCTGCCATC CAAGGAGAGA GACCGGGGCC GAGCCAAAGA CCGCAAGCAC 
CATCATCATC ATCATCACCA CCACGGCTCT TTAGATAAGG AGCACTACGG TCATGAGAGA 
GACAGAGGAG AGTACGGCCA CAGACAGTCC AGAGAGAGAG ACCGCCGCTG GTCCCGCTCA 
CCCAGTGAGG GCAGAGAGTG TCTGACTCAC AGACAGGGCA GTAGTTCAGT AAGCGGCAGT 
CCAGTCCCGT CCACCTCCGG GACCAGCACC CCCCGCCGTG GCCGCCGTCA GCTCCCCCAA 
ACCCCTGCTA CACCCCGGCC ACATGTCACC TACTCACCTG TGGTCCGTAA GCCCATCAGC 
AGTACCCCCC CTCCTGGCCA GCAGAGCCGA CTCCCTACCC CGACCTCCCG GCGCTTCAGT 
CCGACTGGCC CCGAGCCCCC GCTTCCCCCT CATCACCACC CGTCACCACC CCACCACGGC 
TCTCCCCGCT CCGGCCGACA TGCACACTGG GGCCCCGAGC CTGCAGAGAG CGTGGAGGGT 
GACGGCTTCT ATGACGACCA AGACTATGAG TTCAATCACC ACGAGCCTCC ATCCTACGAG 
CAAAGCCCTG TGCAGGGCAA CCCTCACCCC CATTCCCCTA GGACTTCCCG CCACAACACC 
CCTCCTCAGG GCCAAGCGCA CCCACGGCGC ATGCCAAATG GCTACCGTTC GTCTTCGCCC 
TCTCCACACC ACCACGCGCC CGCTCACCCT GGAACTCATA AACCTCCTCA TCCCAGAGGG 
CCCAGAAAGG GCCTCCATGA ACCCTACAGT GAGACTGACG AGGATGACTG GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_009299590.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297865.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X16, mRNA.

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CloneID	ODa43665
Clone ID Related Accession (Same CDS sequence)	XM_009299590.3 , XM_009299590.2
Accession Version	XM_009299590.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X16
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299590.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG 
GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT 
GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC 
CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC 
ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT 
GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT 
GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT 
GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT 
CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA 
AGGCAGTCTC AGCCTATCAC AGATAACAGT CCTATGAAGC GCTCGGCTTC ATCTTTGGGA 
CACGGGCGTG CAGGACGCTC CATGCGAGGT GAGGATTATG CCATGGAGAG GGTTATCCCA 
GAAGAGGGTC ACAGGCATGG GCATCGCCAC CGAGACCGTA GTCACCGGGC GTCAGAACGC 
TCCCTGAGCC GATACACTGA TGCTGACACA GGTCTTGGAA CTGACCTCAG CACAACCACA 
CAATCAGGAG ACCTGCCATC CAAGGAGAGA GACCGGGGCC GAGCCAAAGA CCGCAAGCAC 
CATCATCATC ATCATCACCA CCACGGCTCT TTAGATAAGG AGCACTACGG TCATGAGAGA 
GACAGAGGAG AGTACGGCCA CAGACAGTCC AGAGAGAGAG ACCGCCGCTG GTCCCGCTCA 
CCCAGTGAGG GCAGAGAGTG TCTGACTCAC AGACAGGGCA GTAGTTCAGT AAGCGGCAGT 
CCAGTCCCGT CCACCTCCGG GACCAGCACC CCCCGCCGTG GCCGCCGTCA GCTCCCCCAA 
ACCCCTGCTA CACCCCGGCC ACATGTCACC TACTCACCTG TGGTCCGTAA GCCCATCAGC 
AGTACCCCCC CTCCTGGCCA GCAGAGCCGA CTCCCTACCC CGACCTCCCG GCGCTTCAGT 
CCGACTGGCC CCGAGCCCCC GCTTCCCCCT CATCACCACC CGTCACCACC CCACCACGGC 
TCTCCCCGCT CCGGCCGACA TGCACACTGG GGCCCCGAGC CTGCAGAGAG CGTGGAGGGT 
GACGGCTTCT ATGACGACCA AGACTATGAG TTCAATCACC ACGAGCCTCC ATCCTACGAG 
CAAAGCCCTG TGCAGGGCAA CCCTCACCCC CATTCCCCTA GGACTTCCCG CCACAACACC 
CCTCCTCAGG GCCAAGCGCA CCCACGGCGC ATGCCAAATG GCTACCGTTC GTCTTCGCCC 
TCTCCACACC ACCACGCGCC CGCTCACCCT GGAACTCATA AACCTCCTCA TCCCAGAGGG 
CCCAGAAAGG GCCTCCATGA ACCCTACAGT GAGACTGACG AGGATGACTG GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297865.1
CDS	1468..8424
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGGSGGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGMNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGEGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQNRTPLMFQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_009299590.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299590.2

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG 
GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT 
GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC 
CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC 
ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT 
GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT 
GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT 
GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT 
CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA 
AGGCAGTCTC AGCCTATCAC AGATAACAGT CCTATGAAGC GCTCGGCTTC ATCTTTGGGA 
CACGGGCGTG CAGGACGCTC CATGCGAGGT GAGGATTATG CCATGGAGAG GGTTATCCCA 
GAAGAGGGTC ACAGGCATGG GCATCGCCAC CGAGACCGTA GTCACCGGGC GTCAGAACGC 
TCCCTGAGCC GATACACTGA TGCTGACACA GGTCTTGGAA CTGACCTCAG CACAACCACA 
CAATCAGGAG ACCTGCCATC CAAGGAGAGA GACCGGGGCC GAGCCAAAGA CCGCAAGCAC 
CATCATCATC ATCATCACCA CCACGGCTCT TTAGATAAGG AGCACTACGG TCATGAGAGA 
GACAGAGGAG AGTACGGCCA CAGACAGTCC AGAGAGAGAG ACCGCCGCTG GTCCCGCTCA 
CCCAGTGAGG GCAGAGAGTG TCTGACTCAC AGACAGGGCA GTAGTTCAGT AAGCGGCAGT 
CCAGTCCCGT CCACCTCCGG GACCAGCACC CCCCGCCGTG GCCGCCGTCA GCTCCCCCAA 
ACCCCTGCTA CACCCCGGCC ACATGTCACC TACTCACCTG TGGTCCGTAA GCCCATCAGC 
AGTACCCCCC CTCCTGGCCA GCAGAGCCGA CTCCCTACCC CGACCTCCCG GCGCTTCAGT 
CCGACTGGCC CCGAGCCCCC GCTTCCCCCT CATCACCACC CGTCACCACC CCACCACGGC 
TCTCCCCGCT CCGGCCGACA TGCACACTGG GGCCCCGAGC CTGCAGAGAG CGTGGAGGGT 
GACGGCTTCT ATGACGACCA AGACTATGAG TTCAATCACC ACGAGCCTCC ATCCTACGAG 
CAAAGCCCTG TGCAGGGCAA CCCTCACCCC CATTCCCCTA GGACTTCCCG CCACAACACC 
CCTCCTCAGG GCCAAGCGCA CCCACGGCGC ATGCCAAATG GCTACCGTTC GTCTTCGCCC 
TCTCCACACC ACCACGCGCC CGCTCACCCT GGAACTCATA AACCTCCTCA TCCCAGAGGG 
CCCAGAAAGG GCCTCCATGA ACCCTACAGT GAGACTGACG AGGATGACTG GTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_009299591.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297866.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X17, mRNA.

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CloneID	ODa43666
Clone ID Related Accession (Same CDS sequence)	XM_009299591.2 , XM_009299591.3
Accession Version	XM_009299591.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X17
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299591.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACAGGAAGC AGCAAAGGGA AAGACATCAG TACTATCAAG 
TCTCTACGTG TGCTTCGAGT ACTGCGGCCG CTGAAAACCA TCAAACGTCT GCCAAAACTG 
AAGGCTGTCT TTGACTGTGT GGTGAACTCT CTGAAGAATG TGTTGAACAT CCTCATCGTC 
TACATGCTCT TCATGTTCAT CTTCGCTGTG GTGGCTGTGC AGCTCTTTAA GGGACGTTTC 
TTTTACTGCA CTGATGAATC CAAAGAGTTT GAGCGCGACT GCAGGGGCGA GTATCTGGTC 
TATGAAAGAG ATAACGAAGT GAGGTCACAG AAGCGGGAAT GGAAGAAATA CGATTTCCAC 
TACGACAACG TGCTCTGGGC CCTTCTCACC CTCTTCACCG TTTCTACGGG AGAGGGGTGG 
CCGCAAGTGC TAAAACATTC AGTAGATGCG ACCTACGAGA ATCAGGGCCC GAGCCCAGGT 
TACCGGATGG AAATGTCCAT CTTTTACGTG GTGTACTTCG TGGTCTTCCC CTTCTTCTTC 
GTCAACATCT TCGTGGCTCT TATCATCATT ACTTTCCAGG AGCAAGGAGA CAAAATGATG 
GAGGACTATA GCCTGGAAAA GAATGAGAGA GCCTGCATAG ACTTTGCCAT CAACGCTAAG 
CCGCTCACAC GGCACATGCC CCAGAACAAG CAGACCTTTC AGTACCGCAT GTGGGAGTTT 
GTGGTGTCTC CTCCATTTGA ATACACCATC ATGGCCTTGA TCGCGCTCAA CACTATTGTC 
CTTATGATGA AGTATGATGG AGCATCGCTT ACTTATGAAG ATGTGCTAAA ATACCTGAAC 
ATAGTGTTCA CCTCTCTATT CTCCATGGAG TGCATATTGA AGATCATAGC CTTCGGAGCA 
TTGAACTACT TCAAAGATGC CTGGAACATC TTCGACTGTG TGACTGTACT TGGCAGCATC 
ACTGATATTT TAGTCACAGA GCTGGGGAAC AACTTCATAA ATCTGAGTTT TCTAAGGCTC 
TTCAGAGCTG CTCGTCTCAT TAAGTTGCTC AGGCAGGGTG AAACCATACG CATCCTGCTC 
TGGACTTTTG TCCAGTCTTT CAAGGCTCTG CCGTATGTGT GCCTACTCAT CGCCATGCTG 
TTCTTCATCT ACGCCATCAT TGGCATGCAG CTGTTTGGGA ACATTAAGAT CGAGGAGAAC 
AGTGACAGTG CCATTACCCA GCACAACAAC TTCAGGACTT TTTTCCAGGC CCTAATGCTG 
CTCTTTAGGA GTGCTACAGG TGAGGCATGG CATGACATCA TGCTGTCATG TCTGGGAAAG 
AAGCCGTGTG ATATTCTCTC TGATAACCCG AAGCCAGAAT GCGGGAGCGA GTTTGCCTAC 
CTCTACTTTG TCTCCTTCAT CTTCCTCTGT TCATTTCTGA TGCTGAATCT GTTCGTGGCT 
GTGATCATGG ATAATTTTGA ATACCTGACG CGTGACTCCT CTATCCTGGG ACCCCATCAC 
CTGGACGAGT ATGTGAGGAT CTGGGCAGAA TATGATCCTG CTGCTTGCGG GCGCATTCAT 
TATAAGGATA TGTACAGTTT ATTGCGAGTT ATCGACCCCC CTCTGGGCTT AGGCAAGAAA 
TGTCCTCATA GGGTGGCCTG CAAGAGACTG TTGCGGATGG ATCTGCCGGT TGCCGATGAC 
AACACAGTGC ACTTTAATTC TACCCTTATG GCCTTAATCC GAACCGCTCT GGACATCAAG 
ATCGCCAAGG GAGGTGTAGA TAAGCACCAG ATGGATGCTG AATTAAGGAA GGAGATGATG 
GCGATATGGC CTAATCTCTC TCAGAAGAAC CTGGACCTGC TGGTGACTCC TCACAAGTCA 
GCGACAGACC TGACAGTAGG AAAGATCTAC GCTGCCATGA TGATCATGGA GTATTATCGG 
CAAAGTAAAG CCAAACGGAC ACAAGCTCTC CATGATGAGC AGAATCGAAC GCCATTAATG 
TTTCAGCGTC TGGAGCCGCC ATCTCCTAGC CAGGATGTGG GACCAGGACT CACTGGCCTT 
CCTGACACAC AAATGCACCC TGCAAATCAT CTGCCTGTTG ATGAAAGGAT CCCAGAGAGC 
CAATCATGGG TGACAGCGCG AGCACAGGAG ATTTCTCAAA AAGCAGGCAA CTGGAGTCCA 
GAGGGCCAAC ATCCAGATGA CACCACAGAC AACAGAAGGC AGTCTCAGCC TATCACAGAT 
AACAGTCCTA TGAAGCGCTC GGCTTCATCT TTGGGACACG GGCGTGCAGG ACGCTCCATG 
CGAGGTGAGG ATTATGCCAT GGAGAGGGTT ATCCCAGAAG AGGGTCACAG GCATGGGCAT 
CGCCACCGAG ACCGTAGTCA CCGGGCGTCA GAACGCTCCC TGAGCCGATA CACTGATGCT 
GACACAGGTC TTGGAACTGA CCTCAGCACA ACCACACAAT CAGGAGACCT GCCATCCAAG 
GAGAGAGACC GGGGCCGAGC CAAAGACCGC AAGCACCATC ATCATCATCA TCACCACCAC 
GGCTCTTTAG ATAAGGAGCA CTACGGTCAT GAGAGAGACA GAGGAGAGTA CGGCCACAGA 
CAGTCCAGAG AGAGAGACCG CCGCTGGTCC CGCTCACCCA GTGAGGGCAG AGAGTGTCTG 
ACTCACAGAC AGGGCAGTAG TTCAGTAAGC GGCAGTCCAG TCCCGTCCAC CTCCGGGACC 
AGCACCCCCC GCCGTGGCCG CCGTCAGCTC CCCCAAACCC CTGCTACACC CCGGCCACAT 
GTCACCTACT CACCTGTGGT CCGTAAGCCC ATCAGCAGTA CCCCCCCTCC TGGCCAGCAG 
AGCCGACTCC CTACCCCGAC CTCCCGGCGC TTCAGTCCGA CTGGCCCCGA GCCCCCGCTT 
CCCCCTCATC ACCACCCGTC ACCACCCCAC CACGGCTCTC CCCGCTCCGG CCGACATGCA 
CACTGGGGCC CCGAGCCTGC AGAGAGCGTG GAGGGTGACG GCTTCTATGA CGACCAAGAC 
TATGAGTTCA ATCACCACGA GCCTCCATCC TACGAGCAAA GCCCTGTGCA GGGCAACCCT 
CACCCCCATT CCCCTAGGAC TTCCCGCCAC AACACCCCTC CTCAGGGCCA AGCGCACCCA 
CGGCGCATGC CAAATGGCTA CCGTTCGTCT TCGCCCTCTC CACACCACCA CGCGCCCGCT 
CACCCTGGAA CTCATAAACC TCCTCATCCC AGAGGGCCCA GAAAGGGCCT CCATGAACCC 
TACAGTGAGA CTGACGAGGA TGACTGGTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297866.1
CDS	1467..8399
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQNRTPLMFQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_009299591.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299591.2

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACAGGAAGC AGCAAAGGGA AAGACATCAG TACTATCAAG 
TCTCTACGTG TGCTTCGAGT ACTGCGGCCG CTGAAAACCA TCAAACGTCT GCCAAAACTG 
AAGGCTGTCT TTGACTGTGT GGTGAACTCT CTGAAGAATG TGTTGAACAT CCTCATCGTC 
TACATGCTCT TCATGTTCAT CTTCGCTGTG GTGGCTGTGC AGCTCTTTAA GGGACGTTTC 
TTTTACTGCA CTGATGAATC CAAAGAGTTT GAGCGCGACT GCAGGGGCGA GTATCTGGTC 
TATGAAAGAG ATAACGAAGT GAGGTCACAG AAGCGGGAAT GGAAGAAATA CGATTTCCAC 
TACGACAACG TGCTCTGGGC CCTTCTCACC CTCTTCACCG TTTCTACGGG AGAGGGGTGG 
CCGCAAGTGC TAAAACATTC AGTAGATGCG ACCTACGAGA ATCAGGGCCC GAGCCCAGGT 
TACCGGATGG AAATGTCCAT CTTTTACGTG GTGTACTTCG TGGTCTTCCC CTTCTTCTTC 
GTCAACATCT TCGTGGCTCT TATCATCATT ACTTTCCAGG AGCAAGGAGA CAAAATGATG 
GAGGACTATA GCCTGGAAAA GAATGAGAGA GCCTGCATAG ACTTTGCCAT CAACGCTAAG 
CCGCTCACAC GGCACATGCC CCAGAACAAG CAGACCTTTC AGTACCGCAT GTGGGAGTTT 
GTGGTGTCTC CTCCATTTGA ATACACCATC ATGGCCTTGA TCGCGCTCAA CACTATTGTC 
CTTATGATGA AGTATGATGG AGCATCGCTT ACTTATGAAG ATGTGCTAAA ATACCTGAAC 
ATAGTGTTCA CCTCTCTATT CTCCATGGAG TGCATATTGA AGATCATAGC CTTCGGAGCA 
TTGAACTACT TCAAAGATGC CTGGAACATC TTCGACTGTG TGACTGTACT TGGCAGCATC 
ACTGATATTT TAGTCACAGA GCTGGGGAAC AACTTCATAA ATCTGAGTTT TCTAAGGCTC 
TTCAGAGCTG CTCGTCTCAT TAAGTTGCTC AGGCAGGGTG AAACCATACG CATCCTGCTC 
TGGACTTTTG TCCAGTCTTT CAAGGCTCTG CCGTATGTGT GCCTACTCAT CGCCATGCTG 
TTCTTCATCT ACGCCATCAT TGGCATGCAG CTGTTTGGGA ACATTAAGAT CGAGGAGAAC 
AGTGACAGTG CCATTACCCA GCACAACAAC TTCAGGACTT TTTTCCAGGC CCTAATGCTG 
CTCTTTAGGA GTGCTACAGG TGAGGCATGG CATGACATCA TGCTGTCATG TCTGGGAAAG 
AAGCCGTGTG ATATTCTCTC TGATAACCCG AAGCCAGAAT GCGGGAGCGA GTTTGCCTAC 
CTCTACTTTG TCTCCTTCAT CTTCCTCTGT TCATTTCTGA TGCTGAATCT GTTCGTGGCT 
GTGATCATGG ATAATTTTGA ATACCTGACG CGTGACTCCT CTATCCTGGG ACCCCATCAC 
CTGGACGAGT ATGTGAGGAT CTGGGCAGAA TATGATCCTG CTGCTTGCGG GCGCATTCAT 
TATAAGGATA TGTACAGTTT ATTGCGAGTT ATCGACCCCC CTCTGGGCTT AGGCAAGAAA 
TGTCCTCATA GGGTGGCCTG CAAGAGACTG TTGCGGATGG ATCTGCCGGT TGCCGATGAC 
AACACAGTGC ACTTTAATTC TACCCTTATG GCCTTAATCC GAACCGCTCT GGACATCAAG 
ATCGCCAAGG GAGGTGTAGA TAAGCACCAG ATGGATGCTG AATTAAGGAA GGAGATGATG 
GCGATATGGC CTAATCTCTC TCAGAAGAAC CTGGACCTGC TGGTGACTCC TCACAAGTCA 
GCGACAGACC TGACAGTAGG AAAGATCTAC GCTGCCATGA TGATCATGGA GTATTATCGG 
CAAAGTAAAG CCAAACGGAC ACAAGCTCTC CATGATGAGC AGAATCGAAC GCCATTAATG 
TTTCAGCGTC TGGAGCCGCC ATCTCCTAGC CAGGATGTGG GACCAGGACT CACTGGCCTT 
CCTGACACAC AAATGCACCC TGCAAATCAT CTGCCTGTTG ATGAAAGGAT CCCAGAGAGC 
CAATCATGGG TGACAGCGCG AGCACAGGAG ATTTCTCAAA AAGCAGGCAA CTGGAGTCCA 
GAGGGCCAAC ATCCAGATGA CACCACAGAC AACAGAAGGC AGTCTCAGCC TATCACAGAT 
AACAGTCCTA TGAAGCGCTC GGCTTCATCT TTGGGACACG GGCGTGCAGG ACGCTCCATG 
CGAGGTGAGG ATTATGCCAT GGAGAGGGTT ATCCCAGAAG AGGGTCACAG GCATGGGCAT 
CGCCACCGAG ACCGTAGTCA CCGGGCGTCA GAACGCTCCC TGAGCCGATA CACTGATGCT 
GACACAGGTC TTGGAACTGA CCTCAGCACA ACCACACAAT CAGGAGACCT GCCATCCAAG 
GAGAGAGACC GGGGCCGAGC CAAAGACCGC AAGCACCATC ATCATCATCA TCACCACCAC 
GGCTCTTTAG ATAAGGAGCA CTACGGTCAT GAGAGAGACA GAGGAGAGTA CGGCCACAGA 
CAGTCCAGAG AGAGAGACCG CCGCTGGTCC CGCTCACCCA GTGAGGGCAG AGAGTGTCTG 
ACTCACAGAC AGGGCAGTAG TTCAGTAAGC GGCAGTCCAG TCCCGTCCAC CTCCGGGACC 
AGCACCCCCC GCCGTGGCCG CCGTCAGCTC CCCCAAACCC CTGCTACACC CCGGCCACAT 
GTCACCTACT CACCTGTGGT CCGTAAGCCC ATCAGCAGTA CCCCCCCTCC TGGCCAGCAG 
AGCCGACTCC CTACCCCGAC CTCCCGGCGC TTCAGTCCGA CTGGCCCCGA GCCCCCGCTT 
CCCCCTCATC ACCACCCGTC ACCACCCCAC CACGGCTCTC CCCGCTCCGG CCGACATGCA 
CACTGGGGCC CCGAGCCTGC AGAGAGCGTG GAGGGTGACG GCTTCTATGA CGACCAAGAC 
TATGAGTTCA ATCACCACGA GCCTCCATCC TACGAGCAAA GCCCTGTGCA GGGCAACCCT 
CACCCCCATT CCCCTAGGAC TTCCCGCCAC AACACCCCTC CTCAGGGCCA AGCGCACCCA 
CGGCGCATGC CAAATGGCTA CCGTTCGTCT TCGCCCTCTC CACACCACCA CGCGCCCGCT 
CACCCTGGAA CTCATAAACC TCCTCATCCC AGAGGGCCCA GAAAGGGCCT CCATGAACCC 
TACAGTGAGA CTGACGAGGA TGACTGGTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_009299591.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297866.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X21, mRNA.

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CloneID	ODa43666
Clone ID Related Accession (Same CDS sequence)	XM_009299591.2 , XM_009299591.3
Accession Version	XM_009299591.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009299591.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACAGGAAGC AGCAAAGGGA AAGACATCAG TACTATCAAG 
TCTCTACGTG TGCTTCGAGT ACTGCGGCCG CTGAAAACCA TCAAACGTCT GCCAAAACTG 
AAGGCTGTCT TTGACTGTGT GGTGAACTCT CTGAAGAATG TGTTGAACAT CCTCATCGTC 
TACATGCTCT TCATGTTCAT CTTCGCTGTG GTGGCTGTGC AGCTCTTTAA GGGACGTTTC 
TTTTACTGCA CTGATGAATC CAAAGAGTTT GAGCGCGACT GCAGGGGCGA GTATCTGGTC 
TATGAAAGAG ATAACGAAGT GAGGTCACAG AAGCGGGAAT GGAAGAAATA CGATTTCCAC 
TACGACAACG TGCTCTGGGC CCTTCTCACC CTCTTCACCG TTTCTACGGG AGAGGGGTGG 
CCGCAAGTGC TAAAACATTC AGTAGATGCG ACCTACGAGA ATCAGGGCCC GAGCCCAGGT 
TACCGGATGG AAATGTCCAT CTTTTACGTG GTGTACTTCG TGGTCTTCCC CTTCTTCTTC 
GTCAACATCT TCGTGGCTCT TATCATCATT ACTTTCCAGG AGCAAGGAGA CAAAATGATG 
GAGGACTATA GCCTGGAAAA GAATGAGAGA GCCTGCATAG ACTTTGCCAT CAACGCTAAG 
CCGCTCACAC GGCACATGCC CCAGAACAAG CAGACCTTTC AGTACCGCAT GTGGGAGTTT 
GTGGTGTCTC CTCCATTTGA ATACACCATC ATGGCCTTGA TCGCGCTCAA CACTATTGTC 
CTTATGATGA AGTATGATGG AGCATCGCTT ACTTATGAAG ATGTGCTAAA ATACCTGAAC 
ATAGTGTTCA CCTCTCTATT CTCCATGGAG TGCATATTGA AGATCATAGC CTTCGGAGCA 
TTGAACTACT TCAAAGATGC CTGGAACATC TTCGACTGTG TGACTGTACT TGGCAGCATC 
ACTGATATTT TAGTCACAGA GCTGGGGAAC AACTTCATAA ATCTGAGTTT TCTAAGGCTC 
TTCAGAGCTG CTCGTCTCAT TAAGTTGCTC AGGCAGGGTG AAACCATACG CATCCTGCTC 
TGGACTTTTG TCCAGTCTTT CAAGGCTCTG CCGTATGTGT GCCTACTCAT CGCCATGCTG 
TTCTTCATCT ACGCCATCAT TGGCATGCAG CTGTTTGGGA ACATTAAGAT CGAGGAGAAC 
AGTGACAGTG CCATTACCCA GCACAACAAC TTCAGGACTT TTTTCCAGGC CCTAATGCTG 
CTCTTTAGGA GTGCTACAGG TGAGGCATGG CATGACATCA TGCTGTCATG TCTGGGAAAG 
AAGCCGTGTG ATATTCTCTC TGATAACCCG AAGCCAGAAT GCGGGAGCGA GTTTGCCTAC 
CTCTACTTTG TCTCCTTCAT CTTCCTCTGT TCATTTCTGA TGCTGAATCT GTTCGTGGCT 
GTGATCATGG ATAATTTTGA ATACCTGACG CGTGACTCCT CTATCCTGGG ACCCCATCAC 
CTGGACGAGT ATGTGAGGAT CTGGGCAGAA TATGATCCTG CTGCTTGCGG GCGCATTCAT 
TATAAGGATA TGTACAGTTT ATTGCGAGTT ATCGACCCCC CTCTGGGCTT AGGCAAGAAA 
TGTCCTCATA GGGTGGCCTG CAAGAGACTG TTGCGGATGG ATCTGCCGGT TGCCGATGAC 
AACACAGTGC ACTTTAATTC TACCCTTATG GCCTTAATCC GAACCGCTCT GGACATCAAG 
ATCGCCAAGG GAGGTGTAGA TAAGCACCAG ATGGATGCTG AATTAAGGAA GGAGATGATG 
GCGATATGGC CTAATCTCTC TCAGAAGAAC CTGGACCTGC TGGTGACTCC TCACAAGTCA 
GCGACAGACC TGACAGTAGG AAAGATCTAC GCTGCCATGA TGATCATGGA GTATTATCGG 
CAAAGTAAAG CCAAACGGAC ACAAGCTCTC CATGATGAGC AGAATCGAAC GCCATTAATG 
TTTCAGCGTC TGGAGCCGCC ATCTCCTAGC CAGGATGTGG GACCAGGACT CACTGGCCTT 
CCTGACACAC AAATGCACCC TGCAAATCAT CTGCCTGTTG ATGAAAGGAT CCCAGAGAGC 
CAATCATGGG TGACAGCGCG AGCACAGGAG ATTTCTCAAA AAGCAGGCAA CTGGAGTCCA 
GAGGGCCAAC ATCCAGATGA CACCACAGAC AACAGAAGGC AGTCTCAGCC TATCACAGAT 
AACAGTCCTA TGAAGCGCTC GGCTTCATCT TTGGGACACG GGCGTGCAGG ACGCTCCATG 
CGAGGTGAGG ATTATGCCAT GGAGAGGGTT ATCCCAGAAG AGGGTCACAG GCATGGGCAT 
CGCCACCGAG ACCGTAGTCA CCGGGCGTCA GAACGCTCCC TGAGCCGATA CACTGATGCT 
GACACAGGTC TTGGAACTGA CCTCAGCACA ACCACACAAT CAGGAGACCT GCCATCCAAG 
GAGAGAGACC GGGGCCGAGC CAAAGACCGC AAGCACCATC ATCATCATCA TCACCACCAC 
GGCTCTTTAG ATAAGGAGCA CTACGGTCAT GAGAGAGACA GAGGAGAGTA CGGCCACAGA 
CAGTCCAGAG AGAGAGACCG CCGCTGGTCC CGCTCACCCA GTGAGGGCAG AGAGTGTCTG 
ACTCACAGAC AGGGCAGTAG TTCAGTAAGC GGCAGTCCAG TCCCGTCCAC CTCCGGGACC 
AGCACCCCCC GCCGTGGCCG CCGTCAGCTC CCCCAAACCC CTGCTACACC CCGGCCACAT 
GTCACCTACT CACCTGTGGT CCGTAAGCCC ATCAGCAGTA CCCCCCCTCC TGGCCAGCAG 
AGCCGACTCC CTACCCCGAC CTCCCGGCGC TTCAGTCCGA CTGGCCCCGA GCCCCCGCTT 
CCCCCTCATC ACCACCCGTC ACCACCCCAC CACGGCTCTC CCCGCTCCGG CCGACATGCA 
CACTGGGGCC CCGAGCCTGC AGAGAGCGTG GAGGGTGACG GCTTCTATGA CGACCAAGAC 
TATGAGTTCA ATCACCACGA GCCTCCATCC TACGAGCAAA GCCCTGTGCA GGGCAACCCT 
CACCCCCATT CCCCTAGGAC TTCCCGCCAC AACACCCCTC CTCAGGGCCA AGCGCACCCA 
CGGCGCATGC CAAATGGCTA CCGTTCGTCT TCGCCCTCTC CACACCACCA CGCGCCCGCT 
CACCCTGGAA CTCATAAACC TCCTCATCCC AGAGGGCCCA GAAAGGGCCT CCATGAACCC 
TACAGTGAGA CTGACGAGGA TGACTGGTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297866.1
CDS	1510..8442
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQNRTPLMFQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_009299591.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299591.3

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACAGGAAGC AGCAAAGGGA AAGACATCAG TACTATCAAG 
TCTCTACGTG TGCTTCGAGT ACTGCGGCCG CTGAAAACCA TCAAACGTCT GCCAAAACTG 
AAGGCTGTCT TTGACTGTGT GGTGAACTCT CTGAAGAATG TGTTGAACAT CCTCATCGTC 
TACATGCTCT TCATGTTCAT CTTCGCTGTG GTGGCTGTGC AGCTCTTTAA GGGACGTTTC 
TTTTACTGCA CTGATGAATC CAAAGAGTTT GAGCGCGACT GCAGGGGCGA GTATCTGGTC 
TATGAAAGAG ATAACGAAGT GAGGTCACAG AAGCGGGAAT GGAAGAAATA CGATTTCCAC 
TACGACAACG TGCTCTGGGC CCTTCTCACC CTCTTCACCG TTTCTACGGG AGAGGGGTGG 
CCGCAAGTGC TAAAACATTC AGTAGATGCG ACCTACGAGA ATCAGGGCCC GAGCCCAGGT 
TACCGGATGG AAATGTCCAT CTTTTACGTG GTGTACTTCG TGGTCTTCCC CTTCTTCTTC 
GTCAACATCT TCGTGGCTCT TATCATCATT ACTTTCCAGG AGCAAGGAGA CAAAATGATG 
GAGGACTATA GCCTGGAAAA GAATGAGAGA GCCTGCATAG ACTTTGCCAT CAACGCTAAG 
CCGCTCACAC GGCACATGCC CCAGAACAAG CAGACCTTTC AGTACCGCAT GTGGGAGTTT 
GTGGTGTCTC CTCCATTTGA ATACACCATC ATGGCCTTGA TCGCGCTCAA CACTATTGTC 
CTTATGATGA AGTATGATGG AGCATCGCTT ACTTATGAAG ATGTGCTAAA ATACCTGAAC 
ATAGTGTTCA CCTCTCTATT CTCCATGGAG TGCATATTGA AGATCATAGC CTTCGGAGCA 
TTGAACTACT TCAAAGATGC CTGGAACATC TTCGACTGTG TGACTGTACT TGGCAGCATC 
ACTGATATTT TAGTCACAGA GCTGGGGAAC AACTTCATAA ATCTGAGTTT TCTAAGGCTC 
TTCAGAGCTG CTCGTCTCAT TAAGTTGCTC AGGCAGGGTG AAACCATACG CATCCTGCTC 
TGGACTTTTG TCCAGTCTTT CAAGGCTCTG CCGTATGTGT GCCTACTCAT CGCCATGCTG 
TTCTTCATCT ACGCCATCAT TGGCATGCAG CTGTTTGGGA ACATTAAGAT CGAGGAGAAC 
AGTGACAGTG CCATTACCCA GCACAACAAC TTCAGGACTT TTTTCCAGGC CCTAATGCTG 
CTCTTTAGGA GTGCTACAGG TGAGGCATGG CATGACATCA TGCTGTCATG TCTGGGAAAG 
AAGCCGTGTG ATATTCTCTC TGATAACCCG AAGCCAGAAT GCGGGAGCGA GTTTGCCTAC 
CTCTACTTTG TCTCCTTCAT CTTCCTCTGT TCATTTCTGA TGCTGAATCT GTTCGTGGCT 
GTGATCATGG ATAATTTTGA ATACCTGACG CGTGACTCCT CTATCCTGGG ACCCCATCAC 
CTGGACGAGT ATGTGAGGAT CTGGGCAGAA TATGATCCTG CTGCTTGCGG GCGCATTCAT 
TATAAGGATA TGTACAGTTT ATTGCGAGTT ATCGACCCCC CTCTGGGCTT AGGCAAGAAA 
TGTCCTCATA GGGTGGCCTG CAAGAGACTG TTGCGGATGG ATCTGCCGGT TGCCGATGAC 
AACACAGTGC ACTTTAATTC TACCCTTATG GCCTTAATCC GAACCGCTCT GGACATCAAG 
ATCGCCAAGG GAGGTGTAGA TAAGCACCAG ATGGATGCTG AATTAAGGAA GGAGATGATG 
GCGATATGGC CTAATCTCTC TCAGAAGAAC CTGGACCTGC TGGTGACTCC TCACAAGTCA 
GCGACAGACC TGACAGTAGG AAAGATCTAC GCTGCCATGA TGATCATGGA GTATTATCGG 
CAAAGTAAAG CCAAACGGAC ACAAGCTCTC CATGATGAGC AGAATCGAAC GCCATTAATG 
TTTCAGCGTC TGGAGCCGCC ATCTCCTAGC CAGGATGTGG GACCAGGACT CACTGGCCTT 
CCTGACACAC AAATGCACCC TGCAAATCAT CTGCCTGTTG ATGAAAGGAT CCCAGAGAGC 
CAATCATGGG TGACAGCGCG AGCACAGGAG ATTTCTCAAA AAGCAGGCAA CTGGAGTCCA 
GAGGGCCAAC ATCCAGATGA CACCACAGAC AACAGAAGGC AGTCTCAGCC TATCACAGAT 
AACAGTCCTA TGAAGCGCTC GGCTTCATCT TTGGGACACG GGCGTGCAGG ACGCTCCATG 
CGAGGTGAGG ATTATGCCAT GGAGAGGGTT ATCCCAGAAG AGGGTCACAG GCATGGGCAT 
CGCCACCGAG ACCGTAGTCA CCGGGCGTCA GAACGCTCCC TGAGCCGATA CACTGATGCT 
GACACAGGTC TTGGAACTGA CCTCAGCACA ACCACACAAT CAGGAGACCT GCCATCCAAG 
GAGAGAGACC GGGGCCGAGC CAAAGACCGC AAGCACCATC ATCATCATCA TCACCACCAC 
GGCTCTTTAG ATAAGGAGCA CTACGGTCAT GAGAGAGACA GAGGAGAGTA CGGCCACAGA 
CAGTCCAGAG AGAGAGACCG CCGCTGGTCC CGCTCACCCA GTGAGGGCAG AGAGTGTCTG 
ACTCACAGAC AGGGCAGTAG TTCAGTAAGC GGCAGTCCAG TCCCGTCCAC CTCCGGGACC 
AGCACCCCCC GCCGTGGCCG CCGTCAGCTC CCCCAAACCC CTGCTACACC CCGGCCACAT 
GTCACCTACT CACCTGTGGT CCGTAAGCCC ATCAGCAGTA CCCCCCCTCC TGGCCAGCAG 
AGCCGACTCC CTACCCCGAC CTCCCGGCGC TTCAGTCCGA CTGGCCCCGA GCCCCCGCTT 
CCCCCTCATC ACCACCCGTC ACCACCCCAC CACGGCTCTC CCCGCTCCGG CCGACATGCA 
CACTGGGGCC CCGAGCCTGC AGAGAGCGTG GAGGGTGACG GCTTCTATGA CGACCAAGAC 
TATGAGTTCA ATCACCACGA GCCTCCATCC TACGAGCAAA GCCCTGTGCA GGGCAACCCT 
CACCCCCATT CCCCTAGGAC TTCCCGCCAC AACACCCCTC CTCAGGGCCA AGCGCACCCA 
CGGCGCATGC CAAATGGCTA CCGTTCGTCT TCGCCCTCTC CACACCACCA CGCGCCCGCT 
CACCCTGGAA CTCATAAACC TCCTCATCCC AGAGGGCCCA GAAAGGGCCT CCATGAACCC 
TACAGTGAGA CTGACGAGGA TGACTGGTGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_009299592.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297867.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X20, mRNA.

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CloneID	ODa43667
Clone ID Related Accession (Same CDS sequence)	XM_009299592.2
Accession Version	XM_009299592.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X20
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299592.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGT TTTTTTGTTA TAATCAACCT 
TTATTTGGAT CCAGAGTTTG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297867.1
CDS	1539..6242
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGGSGGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGFFCYNQPLFGSRVC

Target ORF information:

RefSeq Version	XM_009299592.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299592.2

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGT TTTTTTGTTA TAATCAACCT 
TTATTTGGAT CCAGAGTTTG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_009299593.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297868.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X21, mRNA.

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CloneID	ODa43668
Clone ID Related Accession (Same CDS sequence)	XM_009299593.2 , XM_009299593.3
Accession Version	XM_009299593.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X21
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299593.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCTAAGGA GCAGCAGAAG AACCACAAAG GTTGTAAGTC GGTATGGGAG 
CAGCGCACTA GTGAGTTAAG ACGACAGACC CTCGTGAACA GCAGAGAGGC TCTTTATAAT 
GAGTTGGACC CTGAAGACCG TTGGAAGGTC TCATACTCCC GTCACATACG TCCCGACATG 
AAAACTCACC TGGACCGGCC GTTAGTCGTG GACCCTCAGG AGAACCGCAA TAACAACACT 
AACAAAACCC GTCCGGGTGA CGGCCAACCT CAGCATTCCC ATCAGCTCCT ACACAAACAG 
CCCAACTTCA ATGAGGCCGA GACTGGAGGA GGTGGAGGGT CTGAGTCCTG GCCCGCACTG 
GAAAGAGGAG ACTCAACAGG GTCCCGCCGC AGTCTTTTGG GCTCTGAGCA CTATGAGGAG 
GGTAGAGAGT CCCACCGCAG ACACCAGCAC AGCCAGCACT GCAGTCAGGC CCGTCAACAT 
CGCAAAGCTG CAGAGGGCGG TGGAGAGCCT GGAGGGAGGA GACACAGGAG TAAGGCCAAG 
GAAAGAGGAC CTGAGTGTGA GCATTCAGAT GGAGGGGAGC GGAAACACAG ACGGCATCGG 
CATGAGGGTG AGGGGAGGAA AGAGAGAGGG GTACGACACC GAAACAGGAG GGATGGCCAT 
GCAAGTGGCC CGACTCTCTC TACAACCCGT CCCATTCAGA AGACTCTGTC CAGGCAGGAC 
AGTCAGTACA GCGAAGACCT GGACAATGCC ATGAACAACA AACTTGCTAC CCAGCCACAT 
GATAGTCTGC TTAATCTGGC CAACACTGCC CACTCTGCCG GCCTGGCACA TACTGGTACA 
GAGAGCAGCC TCATCCTCAC CAACCCTTCC TCTACCATTG CAAATGTTAC TAGCATCGGT 
CACCTGGGTA TGAAACCTGA GTACACTGCC GTGGACATTC CTCCCATGTT CCCCTCCAGT 
AATGCCATTC TACAGGTCAA TAAGAATGCC AACACGGAGC CTCTGCCAAA GAAGGAAGAC 
ACAAAGGGTG ACGATGATGA TGATGAGAAA GATGACGGAG GGCCTAAACC CATGCCACCC 
TACACCTCAA TGTTCATTCT GACCACCACC AACCCATTTC GGAGATTGTG TCACTATATC 
GTCACACTGA GATATTTTGA GATGTGTATT CTACTGGTTA TTGCTATGAG CAGTATTGCC 
CTGGCTGCTG AAGACCCTGT CTGGCCTGAA TCTCCCCGCA ACAATGTCCT ACGATACTTT 
GACTATGTGT TCACTGGTGT GTTCACCTTT GAGATGCTCA TTAAGATGGT AGATTTAGGT 
CTTGTCCTGC ATCAAGGCTC ATACTTTCGT GATCTGTGGA ACATCCTGGA CTTTATAGTG 
GTTAGTGGTG CCCTGGTGGC GTTCGCCTTC ACAGGAAGCA GCAAAGGGAA AGACATCAGT 
ACTATCAAGT CTCTACGTGT GCTTCGAGTA CTGCGGCCGC TGAAAACCAT CAAACGTCTG 
CCAAAACTGA AGGCTGTCTT TGACTGTGTG GTGAACTCTC TGAAGAATGT GTTGAACATC 
CTCATCGTCT ACATGCTCTT CATGTTCATC TTCGCTGTGG TGGCTGTGCA GCTCTTTAAG 
GGACGTTTCT TTTACTGCAC TGATGAATCC AAAGAGTTTG AGCGCGACTG CAGGGGCGAG 
TATCTGGTCT ATGAAAGAGA TAACGAAGTG AGGTCACAGA AGCGGGAATG GAAGAAATAC 
GATTTCCACT ACGACAACGT GCTCTGGGCC CTTCTCACCC TCTTCACCGT TTCTACGGGA 
GAGGGGTGGC CGCAAGTGCT AAAACATTCA GTAGATGCGA CCTACGAGAA TCAGGGCCCG 
AGCCCAGGTT ACCGGATGGA AATGTCCATC TTTTACGTGG TGTACTTCGT GGTCTTCCCC 
TTCTTCTTCG TCAACATCTT CGTGGCTCTT ATCATCATTA CTTTCCAGGA GCAAGGAGAC 
AAAATGATGG AGGACTATAG CCTGGAAAAG AATGAGAGAG CCTGCATAGA CTTTGCCATC 
AACGCTAAGC CGCTCACACG GCACATGCCC CAGAACAAGC AGACCTTTCA GTACCGCATG 
TGGGAGTTTG TGGTGTCTCC TCCATTTGAA TACACCATCA TGGCCTTGAT CGCGCTCAAC 
ACTATTGTCC TTATGATGAA GTATGATGGA GCATCGCTTA CTTATGAAGA TGTGCTAAAA 
TACCTGAACA TAGTGTTCAC CTCTCTATTC TCCATGGAGT GCATATTGAA GATCATAGCC 
TTCGGAGCAT TGAACTACTT CAAAGATGCC TGGAACATCT TCGACTGTGT GACTGTACTT 
GGCAGCATCA CTGATATTTT AGTCACAGAG CTGGGGAACA ACTTCATAAA TCTGAGTTTT 
CTAAGGCTCT TCAGAGCTGC TCGTCTCATT AAGTTGCTCA GGCAGGGTGA AACCATACGC 
ATCCTGCTCT GGACTTTTGT CCAGTCTTTC AAGGCTCTGC CGTATGTGTG CCTACTCATC 
GCCATGCTGT TCTTCATCTA CGCCATCATT GGCATGCAGC TGTTTGGGAA CATTAAGATC 
GAGGAGAACA GTGACAGTGC CATTACCCAG CACAACAACT TCAGGACTTT TTTCCAGGCC 
CTAATGCTGC TCTTTAGGAG TGCTACAGGT GAGGCATGGC ATGACATCAT GCTGTCATGT 
CTGGGAAAGA AGCCGTGTGA TATTCTCTCT GATAACCCGA AGCCAGAATG CGGGAGCGAG 
TTTGCCTACC TCTACTTTGT CTCCTTCATC TTCCTCTGTT CATTTCTGAT GCTGAATCTG 
TTCGTGGCTG TGATCATGGA TAATTTTGAA TACCTGACGC GTGACTCCTC TATCCTGGGA 
CCCCATCACC TGGACGAGTA TGTGAGGATC TGGGCAGAAT ATGATCCTGC TGCTTGCGGG 
CGCATTCATT ATAAGGATAT GTACAGTTTA TTGCGAGTTA TCGACCCCCC TCTGGGCTTA 
GGCAAGAAAT GTCCTCATAG GGTGGCCTGC AAGAGACTGT TGCGGATGGA TCTGCCGGTT 
GCCGATGACA ACACAGTGCA CTTTAATTCT ACCCTTATGG CCTTAATCCG AACCGCTCTG 
GACATCAAGA TCGCCAAGGG AGGTGTAGAT AAGCACCAGA TGGATGCTGA ATTAAGGAAG 
GAGATGATGG CGATATGGCC TAATCTCTCT CAGAAGAACC TGGACCTGCT GGTGACTCCT 
CACAAGTCAG CGACAGACCT GACAGTAGGA AAGATCTACG CTGCCATGAT GATCATGGAG 
TATTATCGGC AAAGTAAAGC CAAACGGACA CAAGCTCTCC ATGATGAGCA GAATCGAACG 
CCATTAATGT TTCAGCGTCT GGAGCCGCCA TCTCCTAGCC AGGATGTGGG ACCAGGACTC 
ACTGGCCTTC CTGACACACA AATGCACCCT GCAAATCATC TGCCTGTTGA TGAAAGGATC 
CCAGAGAGCC AATCATGGGT GACAGCGCGA GCACAGGAGA TTTCTCAAAA AGCAGGCAAC 
TGGAGTCCAG AGGGCCAACA TCCAGATGAC ACCACAGACA ACAGAAGGCA GTCTCAGCCT 
ATCACAGATA ACAGTCCTAT GAAGCGCTCG GCTTCATCTT TGGGACACGG GCGTGCAGGA 
CGCTCCATGC GAGGTGAGGA TTATGCCATG GAGAGGGTTA TCCCAGAAGA GGGTCACAGG 
CATGGGCATC GCCACCGAGA CCGTAGTCAC CGGGCGTCAG AACGCTCCCT GAGCCGATAC 
ACTGATGCTG ACACAGGTCT TGGAACTGAC CTCAGCACAA CCACACAATC AGGAGACCTG 
CCATCCAAGG AGAGAGACCG GGGCCGAGCC AAAGACCGCA AGCACCATCA TCATCATCAT 
CACCACCACG GCTCTTTAGA TAAGGAGCAC TACGGTCATG AGAGAGACAG AGGAGAGTAC 
GGCCACAGAC AGTCCAGAGA GAGAGACCGC CGCTGGTCCC GCTCACCCAG TGAGGGCAGA 
GAGTGTCTGA CTCACAGACA GGGCAGTAGT TCAGTAAGCG GCAGTCCAGT CCCGTCCACC 
TCCGGGACCA GCACCCCCCG CCGTGGCCGC CGTCAGCTCC CCCAAACCCC TGCTACACCC 
CGGCCACATG TCACCTACTC ACCTGTGGTC CGTAAGCCCA TCAGCAGTAC CCCCCCTCCT 
GGCCAGCAGA GCCGACTCCC TACCCCGACC TCCCGGCGCT TCAGTCCGAC TGGCCCCGAG 
CCCCCGCTTC CCCCTCATCA CCACCCGTCA CCACCCCACC ACGGCTCTCC CCGCTCCGGC 
CGACATGCAC ACTGGGGCCC CGAGCCTGCA GAGAGCGTGG AGGGTGACGG CTTCTATGAC 
GACCAAGACT ATGAGTTCAA TCACCACGAG CCTCCATCCT ACGAGCAAAG CCCTGTGCAG 
GGCAACCCTC ACCCCCATTC CCCTAGGACT TCCCGCCACA ACACCCCTCC TCAGGGCCAA 
GCGCACCCAC GGCGCATGCC AAATGGCTAC CGTTCGTCTT CGCCCTCTCC ACACCACCAC 
GCGCCCGCTC ACCCTGGAAC TCATAAACCT CCTCATCCCA GAGGGCCCAG AAAGGGCCTC 
CATGAACCCT ACAGTGAGAC TGACGAGGAT GACTGGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297868.1
CDS	1467..8348
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQNRTPLMFQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_009299593.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299593.2

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCTAAGGA GCAGCAGAAG AACCACAAAG GTTGTAAGTC GGTATGGGAG 
CAGCGCACTA GTGAGTTAAG ACGACAGACC CTCGTGAACA GCAGAGAGGC TCTTTATAAT 
GAGTTGGACC CTGAAGACCG TTGGAAGGTC TCATACTCCC GTCACATACG TCCCGACATG 
AAAACTCACC TGGACCGGCC GTTAGTCGTG GACCCTCAGG AGAACCGCAA TAACAACACT 
AACAAAACCC GTCCGGGTGA CGGCCAACCT CAGCATTCCC ATCAGCTCCT ACACAAACAG 
CCCAACTTCA ATGAGGCCGA GACTGGAGGA GGTGGAGGGT CTGAGTCCTG GCCCGCACTG 
GAAAGAGGAG ACTCAACAGG GTCCCGCCGC AGTCTTTTGG GCTCTGAGCA CTATGAGGAG 
GGTAGAGAGT CCCACCGCAG ACACCAGCAC AGCCAGCACT GCAGTCAGGC CCGTCAACAT 
CGCAAAGCTG CAGAGGGCGG TGGAGAGCCT GGAGGGAGGA GACACAGGAG TAAGGCCAAG 
GAAAGAGGAC CTGAGTGTGA GCATTCAGAT GGAGGGGAGC GGAAACACAG ACGGCATCGG 
CATGAGGGTG AGGGGAGGAA AGAGAGAGGG GTACGACACC GAAACAGGAG GGATGGCCAT 
GCAAGTGGCC CGACTCTCTC TACAACCCGT CCCATTCAGA AGACTCTGTC CAGGCAGGAC 
AGTCAGTACA GCGAAGACCT GGACAATGCC ATGAACAACA AACTTGCTAC CCAGCCACAT 
GATAGTCTGC TTAATCTGGC CAACACTGCC CACTCTGCCG GCCTGGCACA TACTGGTACA 
GAGAGCAGCC TCATCCTCAC CAACCCTTCC TCTACCATTG CAAATGTTAC TAGCATCGGT 
CACCTGGGTA TGAAACCTGA GTACACTGCC GTGGACATTC CTCCCATGTT CCCCTCCAGT 
AATGCCATTC TACAGGTCAA TAAGAATGCC AACACGGAGC CTCTGCCAAA GAAGGAAGAC 
ACAAAGGGTG ACGATGATGA TGATGAGAAA GATGACGGAG GGCCTAAACC CATGCCACCC 
TACACCTCAA TGTTCATTCT GACCACCACC AACCCATTTC GGAGATTGTG TCACTATATC 
GTCACACTGA GATATTTTGA GATGTGTATT CTACTGGTTA TTGCTATGAG CAGTATTGCC 
CTGGCTGCTG AAGACCCTGT CTGGCCTGAA TCTCCCCGCA ACAATGTCCT ACGATACTTT 
GACTATGTGT TCACTGGTGT GTTCACCTTT GAGATGCTCA TTAAGATGGT AGATTTAGGT 
CTTGTCCTGC ATCAAGGCTC ATACTTTCGT GATCTGTGGA ACATCCTGGA CTTTATAGTG 
GTTAGTGGTG CCCTGGTGGC GTTCGCCTTC ACAGGAAGCA GCAAAGGGAA AGACATCAGT 
ACTATCAAGT CTCTACGTGT GCTTCGAGTA CTGCGGCCGC TGAAAACCAT CAAACGTCTG 
CCAAAACTGA AGGCTGTCTT TGACTGTGTG GTGAACTCTC TGAAGAATGT GTTGAACATC 
CTCATCGTCT ACATGCTCTT CATGTTCATC TTCGCTGTGG TGGCTGTGCA GCTCTTTAAG 
GGACGTTTCT TTTACTGCAC TGATGAATCC AAAGAGTTTG AGCGCGACTG CAGGGGCGAG 
TATCTGGTCT ATGAAAGAGA TAACGAAGTG AGGTCACAGA AGCGGGAATG GAAGAAATAC 
GATTTCCACT ACGACAACGT GCTCTGGGCC CTTCTCACCC TCTTCACCGT TTCTACGGGA 
GAGGGGTGGC CGCAAGTGCT AAAACATTCA GTAGATGCGA CCTACGAGAA TCAGGGCCCG 
AGCCCAGGTT ACCGGATGGA AATGTCCATC TTTTACGTGG TGTACTTCGT GGTCTTCCCC 
TTCTTCTTCG TCAACATCTT CGTGGCTCTT ATCATCATTA CTTTCCAGGA GCAAGGAGAC 
AAAATGATGG AGGACTATAG CCTGGAAAAG AATGAGAGAG CCTGCATAGA CTTTGCCATC 
AACGCTAAGC CGCTCACACG GCACATGCCC CAGAACAAGC AGACCTTTCA GTACCGCATG 
TGGGAGTTTG TGGTGTCTCC TCCATTTGAA TACACCATCA TGGCCTTGAT CGCGCTCAAC 
ACTATTGTCC TTATGATGAA GTATGATGGA GCATCGCTTA CTTATGAAGA TGTGCTAAAA 
TACCTGAACA TAGTGTTCAC CTCTCTATTC TCCATGGAGT GCATATTGAA GATCATAGCC 
TTCGGAGCAT TGAACTACTT CAAAGATGCC TGGAACATCT TCGACTGTGT GACTGTACTT 
GGCAGCATCA CTGATATTTT AGTCACAGAG CTGGGGAACA ACTTCATAAA TCTGAGTTTT 
CTAAGGCTCT TCAGAGCTGC TCGTCTCATT AAGTTGCTCA GGCAGGGTGA AACCATACGC 
ATCCTGCTCT GGACTTTTGT CCAGTCTTTC AAGGCTCTGC CGTATGTGTG CCTACTCATC 
GCCATGCTGT TCTTCATCTA CGCCATCATT GGCATGCAGC TGTTTGGGAA CATTAAGATC 
GAGGAGAACA GTGACAGTGC CATTACCCAG CACAACAACT TCAGGACTTT TTTCCAGGCC 
CTAATGCTGC TCTTTAGGAG TGCTACAGGT GAGGCATGGC ATGACATCAT GCTGTCATGT 
CTGGGAAAGA AGCCGTGTGA TATTCTCTCT GATAACCCGA AGCCAGAATG CGGGAGCGAG 
TTTGCCTACC TCTACTTTGT CTCCTTCATC TTCCTCTGTT CATTTCTGAT GCTGAATCTG 
TTCGTGGCTG TGATCATGGA TAATTTTGAA TACCTGACGC GTGACTCCTC TATCCTGGGA 
CCCCATCACC TGGACGAGTA TGTGAGGATC TGGGCAGAAT ATGATCCTGC TGCTTGCGGG 
CGCATTCATT ATAAGGATAT GTACAGTTTA TTGCGAGTTA TCGACCCCCC TCTGGGCTTA 
GGCAAGAAAT GTCCTCATAG GGTGGCCTGC AAGAGACTGT TGCGGATGGA TCTGCCGGTT 
GCCGATGACA ACACAGTGCA CTTTAATTCT ACCCTTATGG CCTTAATCCG AACCGCTCTG 
GACATCAAGA TCGCCAAGGG AGGTGTAGAT AAGCACCAGA TGGATGCTGA ATTAAGGAAG 
GAGATGATGG CGATATGGCC TAATCTCTCT CAGAAGAACC TGGACCTGCT GGTGACTCCT 
CACAAGTCAG CGACAGACCT GACAGTAGGA AAGATCTACG CTGCCATGAT GATCATGGAG 
TATTATCGGC AAAGTAAAGC CAAACGGACA CAAGCTCTCC ATGATGAGCA GAATCGAACG 
CCATTAATGT TTCAGCGTCT GGAGCCGCCA TCTCCTAGCC AGGATGTGGG ACCAGGACTC 
ACTGGCCTTC CTGACACACA AATGCACCCT GCAAATCATC TGCCTGTTGA TGAAAGGATC 
CCAGAGAGCC AATCATGGGT GACAGCGCGA GCACAGGAGA TTTCTCAAAA AGCAGGCAAC 
TGGAGTCCAG AGGGCCAACA TCCAGATGAC ACCACAGACA ACAGAAGGCA GTCTCAGCCT 
ATCACAGATA ACAGTCCTAT GAAGCGCTCG GCTTCATCTT TGGGACACGG GCGTGCAGGA 
CGCTCCATGC GAGGTGAGGA TTATGCCATG GAGAGGGTTA TCCCAGAAGA GGGTCACAGG 
CATGGGCATC GCCACCGAGA CCGTAGTCAC CGGGCGTCAG AACGCTCCCT GAGCCGATAC 
ACTGATGCTG ACACAGGTCT TGGAACTGAC CTCAGCACAA CCACACAATC AGGAGACCTG 
CCATCCAAGG AGAGAGACCG GGGCCGAGCC AAAGACCGCA AGCACCATCA TCATCATCAT 
CACCACCACG GCTCTTTAGA TAAGGAGCAC TACGGTCATG AGAGAGACAG AGGAGAGTAC 
GGCCACAGAC AGTCCAGAGA GAGAGACCGC CGCTGGTCCC GCTCACCCAG TGAGGGCAGA 
GAGTGTCTGA CTCACAGACA GGGCAGTAGT TCAGTAAGCG GCAGTCCAGT CCCGTCCACC 
TCCGGGACCA GCACCCCCCG CCGTGGCCGC CGTCAGCTCC CCCAAACCCC TGCTACACCC 
CGGCCACATG TCACCTACTC ACCTGTGGTC CGTAAGCCCA TCAGCAGTAC CCCCCCTCCT 
GGCCAGCAGA GCCGACTCCC TACCCCGACC TCCCGGCGCT TCAGTCCGAC TGGCCCCGAG 
CCCCCGCTTC CCCCTCATCA CCACCCGTCA CCACCCCACC ACGGCTCTCC CCGCTCCGGC 
CGACATGCAC ACTGGGGCCC CGAGCCTGCA GAGAGCGTGG AGGGTGACGG CTTCTATGAC 
GACCAAGACT ATGAGTTCAA TCACCACGAG CCTCCATCCT ACGAGCAAAG CCCTGTGCAG 
GGCAACCCTC ACCCCCATTC CCCTAGGACT TCCCGCCACA ACACCCCTCC TCAGGGCCAA 
GCGCACCCAC GGCGCATGCC AAATGGCTAC CGTTCGTCTT CGCCCTCTCC ACACCACCAC 
GCGCCCGCTC ACCCTGGAAC TCATAAACCT CCTCATCCCA GAGGGCCCAG AAAGGGCCTC 
CATGAACCCT ACAGTGAGAC TGACGAGGAT GACTGGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_009299593.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297868.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X26, mRNA.

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CloneID	ODa43668
Clone ID Related Accession (Same CDS sequence)	XM_009299593.2 , XM_009299593.3
Accession Version	XM_009299593.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009299593.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCTAAGGA GCAGCAGAAG AACCACAAAG GTTGTAAGTC GGTATGGGAG 
CAGCGCACTA GTGAGTTAAG ACGACAGACC CTCGTGAACA GCAGAGAGGC TCTTTATAAT 
GAGTTGGACC CTGAAGACCG TTGGAAGGTC TCATACTCCC GTCACATACG TCCCGACATG 
AAAACTCACC TGGACCGGCC GTTAGTCGTG GACCCTCAGG AGAACCGCAA TAACAACACT 
AACAAAACCC GTCCGGGTGA CGGCCAACCT CAGCATTCCC ATCAGCTCCT ACACAAACAG 
CCCAACTTCA ATGAGGCCGA GACTGGAGGA GGTGGAGGGT CTGAGTCCTG GCCCGCACTG 
GAAAGAGGAG ACTCAACAGG GTCCCGCCGC AGTCTTTTGG GCTCTGAGCA CTATGAGGAG 
GGTAGAGAGT CCCACCGCAG ACACCAGCAC AGCCAGCACT GCAGTCAGGC CCGTCAACAT 
CGCAAAGCTG CAGAGGGCGG TGGAGAGCCT GGAGGGAGGA GACACAGGAG TAAGGCCAAG 
GAAAGAGGAC CTGAGTGTGA GCATTCAGAT GGAGGGGAGC GGAAACACAG ACGGCATCGG 
CATGAGGGTG AGGGGAGGAA AGAGAGAGGG GTACGACACC GAAACAGGAG GGATGGCCAT 
GCAAGTGGCC CGACTCTCTC TACAACCCGT CCCATTCAGA AGACTCTGTC CAGGCAGGAC 
AGTCAGTACA GCGAAGACCT GGACAATGCC ATGAACAACA AACTTGCTAC CCAGCCACAT 
GATAGTCTGC TTAATCTGGC CAACACTGCC CACTCTGCCG GCCTGGCACA TACTGGTACA 
GAGAGCAGCC TCATCCTCAC CAACCCTTCC TCTACCATTG CAAATGTTAC TAGCATCGGT 
CACCTGGGTA TGAAACCTGA GTACACTGCC GTGGACATTC CTCCCATGTT CCCCTCCAGT 
AATGCCATTC TACAGGTCAA TAAGAATGCC AACACGGAGC CTCTGCCAAA GAAGGAAGAC 
ACAAAGGGTG ACGATGATGA TGATGAGAAA GATGACGGAG GGCCTAAACC CATGCCACCC 
TACACCTCAA TGTTCATTCT GACCACCACC AACCCATTTC GGAGATTGTG TCACTATATC 
GTCACACTGA GATATTTTGA GATGTGTATT CTACTGGTTA TTGCTATGAG CAGTATTGCC 
CTGGCTGCTG AAGACCCTGT CTGGCCTGAA TCTCCCCGCA ACAATGTCCT ACGATACTTT 
GACTATGTGT TCACTGGTGT GTTCACCTTT GAGATGCTCA TTAAGATGGT AGATTTAGGT 
CTTGTCCTGC ATCAAGGCTC ATACTTTCGT GATCTGTGGA ACATCCTGGA CTTTATAGTG 
GTTAGTGGTG CCCTGGTGGC GTTCGCCTTC ACAGGAAGCA GCAAAGGGAA AGACATCAGT 
ACTATCAAGT CTCTACGTGT GCTTCGAGTA CTGCGGCCGC TGAAAACCAT CAAACGTCTG 
CCAAAACTGA AGGCTGTCTT TGACTGTGTG GTGAACTCTC TGAAGAATGT GTTGAACATC 
CTCATCGTCT ACATGCTCTT CATGTTCATC TTCGCTGTGG TGGCTGTGCA GCTCTTTAAG 
GGACGTTTCT TTTACTGCAC TGATGAATCC AAAGAGTTTG AGCGCGACTG CAGGGGCGAG 
TATCTGGTCT ATGAAAGAGA TAACGAAGTG AGGTCACAGA AGCGGGAATG GAAGAAATAC 
GATTTCCACT ACGACAACGT GCTCTGGGCC CTTCTCACCC TCTTCACCGT TTCTACGGGA 
GAGGGGTGGC CGCAAGTGCT AAAACATTCA GTAGATGCGA CCTACGAGAA TCAGGGCCCG 
AGCCCAGGTT ACCGGATGGA AATGTCCATC TTTTACGTGG TGTACTTCGT GGTCTTCCCC 
TTCTTCTTCG TCAACATCTT CGTGGCTCTT ATCATCATTA CTTTCCAGGA GCAAGGAGAC 
AAAATGATGG AGGACTATAG CCTGGAAAAG AATGAGAGAG CCTGCATAGA CTTTGCCATC 
AACGCTAAGC CGCTCACACG GCACATGCCC CAGAACAAGC AGACCTTTCA GTACCGCATG 
TGGGAGTTTG TGGTGTCTCC TCCATTTGAA TACACCATCA TGGCCTTGAT CGCGCTCAAC 
ACTATTGTCC TTATGATGAA GTATGATGGA GCATCGCTTA CTTATGAAGA TGTGCTAAAA 
TACCTGAACA TAGTGTTCAC CTCTCTATTC TCCATGGAGT GCATATTGAA GATCATAGCC 
TTCGGAGCAT TGAACTACTT CAAAGATGCC TGGAACATCT TCGACTGTGT GACTGTACTT 
GGCAGCATCA CTGATATTTT AGTCACAGAG CTGGGGAACA ACTTCATAAA TCTGAGTTTT 
CTAAGGCTCT TCAGAGCTGC TCGTCTCATT AAGTTGCTCA GGCAGGGTGA AACCATACGC 
ATCCTGCTCT GGACTTTTGT CCAGTCTTTC AAGGCTCTGC CGTATGTGTG CCTACTCATC 
GCCATGCTGT TCTTCATCTA CGCCATCATT GGCATGCAGC TGTTTGGGAA CATTAAGATC 
GAGGAGAACA GTGACAGTGC CATTACCCAG CACAACAACT TCAGGACTTT TTTCCAGGCC 
CTAATGCTGC TCTTTAGGAG TGCTACAGGT GAGGCATGGC ATGACATCAT GCTGTCATGT 
CTGGGAAAGA AGCCGTGTGA TATTCTCTCT GATAACCCGA AGCCAGAATG CGGGAGCGAG 
TTTGCCTACC TCTACTTTGT CTCCTTCATC TTCCTCTGTT CATTTCTGAT GCTGAATCTG 
TTCGTGGCTG TGATCATGGA TAATTTTGAA TACCTGACGC GTGACTCCTC TATCCTGGGA 
CCCCATCACC TGGACGAGTA TGTGAGGATC TGGGCAGAAT ATGATCCTGC TGCTTGCGGG 
CGCATTCATT ATAAGGATAT GTACAGTTTA TTGCGAGTTA TCGACCCCCC TCTGGGCTTA 
GGCAAGAAAT GTCCTCATAG GGTGGCCTGC AAGAGACTGT TGCGGATGGA TCTGCCGGTT 
GCCGATGACA ACACAGTGCA CTTTAATTCT ACCCTTATGG CCTTAATCCG AACCGCTCTG 
GACATCAAGA TCGCCAAGGG AGGTGTAGAT AAGCACCAGA TGGATGCTGA ATTAAGGAAG 
GAGATGATGG CGATATGGCC TAATCTCTCT CAGAAGAACC TGGACCTGCT GGTGACTCCT 
CACAAGTCAG CGACAGACCT GACAGTAGGA AAGATCTACG CTGCCATGAT GATCATGGAG 
TATTATCGGC AAAGTAAAGC CAAACGGACA CAAGCTCTCC ATGATGAGCA GAATCGAACG 
CCATTAATGT TTCAGCGTCT GGAGCCGCCA TCTCCTAGCC AGGATGTGGG ACCAGGACTC 
ACTGGCCTTC CTGACACACA AATGCACCCT GCAAATCATC TGCCTGTTGA TGAAAGGATC 
CCAGAGAGCC AATCATGGGT GACAGCGCGA GCACAGGAGA TTTCTCAAAA AGCAGGCAAC 
TGGAGTCCAG AGGGCCAACA TCCAGATGAC ACCACAGACA ACAGAAGGCA GTCTCAGCCT 
ATCACAGATA ACAGTCCTAT GAAGCGCTCG GCTTCATCTT TGGGACACGG GCGTGCAGGA 
CGCTCCATGC GAGGTGAGGA TTATGCCATG GAGAGGGTTA TCCCAGAAGA GGGTCACAGG 
CATGGGCATC GCCACCGAGA CCGTAGTCAC CGGGCGTCAG AACGCTCCCT GAGCCGATAC 
ACTGATGCTG ACACAGGTCT TGGAACTGAC CTCAGCACAA CCACACAATC AGGAGACCTG 
CCATCCAAGG AGAGAGACCG GGGCCGAGCC AAAGACCGCA AGCACCATCA TCATCATCAT 
CACCACCACG GCTCTTTAGA TAAGGAGCAC TACGGTCATG AGAGAGACAG AGGAGAGTAC 
GGCCACAGAC AGTCCAGAGA GAGAGACCGC CGCTGGTCCC GCTCACCCAG TGAGGGCAGA 
GAGTGTCTGA CTCACAGACA GGGCAGTAGT TCAGTAAGCG GCAGTCCAGT CCCGTCCACC 
TCCGGGACCA GCACCCCCCG CCGTGGCCGC CGTCAGCTCC CCCAAACCCC TGCTACACCC 
CGGCCACATG TCACCTACTC ACCTGTGGTC CGTAAGCCCA TCAGCAGTAC CCCCCCTCCT 
GGCCAGCAGA GCCGACTCCC TACCCCGACC TCCCGGCGCT TCAGTCCGAC TGGCCCCGAG 
CCCCCGCTTC CCCCTCATCA CCACCCGTCA CCACCCCACC ACGGCTCTCC CCGCTCCGGC 
CGACATGCAC ACTGGGGCCC CGAGCCTGCA GAGAGCGTGG AGGGTGACGG CTTCTATGAC 
GACCAAGACT ATGAGTTCAA TCACCACGAG CCTCCATCCT ACGAGCAAAG CCCTGTGCAG 
GGCAACCCTC ACCCCCATTC CCCTAGGACT TCCCGCCACA ACACCCCTCC TCAGGGCCAA 
GCGCACCCAC GGCGCATGCC AAATGGCTAC CGTTCGTCTT CGCCCTCTCC ACACCACCAC 
GCGCCCGCTC ACCCTGGAAC TCATAAACCT CCTCATCCCA GAGGGCCCAG AAAGGGCCTC 
CATGAACCCT ACAGTGAGAC TGACGAGGAT GACTGGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297868.1
CDS	1507..8388
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQNRTPLMFQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_009299593.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299593.3

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCTAAGGA GCAGCAGAAG AACCACAAAG GTTGTAAGTC GGTATGGGAG 
CAGCGCACTA GTGAGTTAAG ACGACAGACC CTCGTGAACA GCAGAGAGGC TCTTTATAAT 
GAGTTGGACC CTGAAGACCG TTGGAAGGTC TCATACTCCC GTCACATACG TCCCGACATG 
AAAACTCACC TGGACCGGCC GTTAGTCGTG GACCCTCAGG AGAACCGCAA TAACAACACT 
AACAAAACCC GTCCGGGTGA CGGCCAACCT CAGCATTCCC ATCAGCTCCT ACACAAACAG 
CCCAACTTCA ATGAGGCCGA GACTGGAGGA GGTGGAGGGT CTGAGTCCTG GCCCGCACTG 
GAAAGAGGAG ACTCAACAGG GTCCCGCCGC AGTCTTTTGG GCTCTGAGCA CTATGAGGAG 
GGTAGAGAGT CCCACCGCAG ACACCAGCAC AGCCAGCACT GCAGTCAGGC CCGTCAACAT 
CGCAAAGCTG CAGAGGGCGG TGGAGAGCCT GGAGGGAGGA GACACAGGAG TAAGGCCAAG 
GAAAGAGGAC CTGAGTGTGA GCATTCAGAT GGAGGGGAGC GGAAACACAG ACGGCATCGG 
CATGAGGGTG AGGGGAGGAA AGAGAGAGGG GTACGACACC GAAACAGGAG GGATGGCCAT 
GCAAGTGGCC CGACTCTCTC TACAACCCGT CCCATTCAGA AGACTCTGTC CAGGCAGGAC 
AGTCAGTACA GCGAAGACCT GGACAATGCC ATGAACAACA AACTTGCTAC CCAGCCACAT 
GATAGTCTGC TTAATCTGGC CAACACTGCC CACTCTGCCG GCCTGGCACA TACTGGTACA 
GAGAGCAGCC TCATCCTCAC CAACCCTTCC TCTACCATTG CAAATGTTAC TAGCATCGGT 
CACCTGGGTA TGAAACCTGA GTACACTGCC GTGGACATTC CTCCCATGTT CCCCTCCAGT 
AATGCCATTC TACAGGTCAA TAAGAATGCC AACACGGAGC CTCTGCCAAA GAAGGAAGAC 
ACAAAGGGTG ACGATGATGA TGATGAGAAA GATGACGGAG GGCCTAAACC CATGCCACCC 
TACACCTCAA TGTTCATTCT GACCACCACC AACCCATTTC GGAGATTGTG TCACTATATC 
GTCACACTGA GATATTTTGA GATGTGTATT CTACTGGTTA TTGCTATGAG CAGTATTGCC 
CTGGCTGCTG AAGACCCTGT CTGGCCTGAA TCTCCCCGCA ACAATGTCCT ACGATACTTT 
GACTATGTGT TCACTGGTGT GTTCACCTTT GAGATGCTCA TTAAGATGGT AGATTTAGGT 
CTTGTCCTGC ATCAAGGCTC ATACTTTCGT GATCTGTGGA ACATCCTGGA CTTTATAGTG 
GTTAGTGGTG CCCTGGTGGC GTTCGCCTTC ACAGGAAGCA GCAAAGGGAA AGACATCAGT 
ACTATCAAGT CTCTACGTGT GCTTCGAGTA CTGCGGCCGC TGAAAACCAT CAAACGTCTG 
CCAAAACTGA AGGCTGTCTT TGACTGTGTG GTGAACTCTC TGAAGAATGT GTTGAACATC 
CTCATCGTCT ACATGCTCTT CATGTTCATC TTCGCTGTGG TGGCTGTGCA GCTCTTTAAG 
GGACGTTTCT TTTACTGCAC TGATGAATCC AAAGAGTTTG AGCGCGACTG CAGGGGCGAG 
TATCTGGTCT ATGAAAGAGA TAACGAAGTG AGGTCACAGA AGCGGGAATG GAAGAAATAC 
GATTTCCACT ACGACAACGT GCTCTGGGCC CTTCTCACCC TCTTCACCGT TTCTACGGGA 
GAGGGGTGGC CGCAAGTGCT AAAACATTCA GTAGATGCGA CCTACGAGAA TCAGGGCCCG 
AGCCCAGGTT ACCGGATGGA AATGTCCATC TTTTACGTGG TGTACTTCGT GGTCTTCCCC 
TTCTTCTTCG TCAACATCTT CGTGGCTCTT ATCATCATTA CTTTCCAGGA GCAAGGAGAC 
AAAATGATGG AGGACTATAG CCTGGAAAAG AATGAGAGAG CCTGCATAGA CTTTGCCATC 
AACGCTAAGC CGCTCACACG GCACATGCCC CAGAACAAGC AGACCTTTCA GTACCGCATG 
TGGGAGTTTG TGGTGTCTCC TCCATTTGAA TACACCATCA TGGCCTTGAT CGCGCTCAAC 
ACTATTGTCC TTATGATGAA GTATGATGGA GCATCGCTTA CTTATGAAGA TGTGCTAAAA 
TACCTGAACA TAGTGTTCAC CTCTCTATTC TCCATGGAGT GCATATTGAA GATCATAGCC 
TTCGGAGCAT TGAACTACTT CAAAGATGCC TGGAACATCT TCGACTGTGT GACTGTACTT 
GGCAGCATCA CTGATATTTT AGTCACAGAG CTGGGGAACA ACTTCATAAA TCTGAGTTTT 
CTAAGGCTCT TCAGAGCTGC TCGTCTCATT AAGTTGCTCA GGCAGGGTGA AACCATACGC 
ATCCTGCTCT GGACTTTTGT CCAGTCTTTC AAGGCTCTGC CGTATGTGTG CCTACTCATC 
GCCATGCTGT TCTTCATCTA CGCCATCATT GGCATGCAGC TGTTTGGGAA CATTAAGATC 
GAGGAGAACA GTGACAGTGC CATTACCCAG CACAACAACT TCAGGACTTT TTTCCAGGCC 
CTAATGCTGC TCTTTAGGAG TGCTACAGGT GAGGCATGGC ATGACATCAT GCTGTCATGT 
CTGGGAAAGA AGCCGTGTGA TATTCTCTCT GATAACCCGA AGCCAGAATG CGGGAGCGAG 
TTTGCCTACC TCTACTTTGT CTCCTTCATC TTCCTCTGTT CATTTCTGAT GCTGAATCTG 
TTCGTGGCTG TGATCATGGA TAATTTTGAA TACCTGACGC GTGACTCCTC TATCCTGGGA 
CCCCATCACC TGGACGAGTA TGTGAGGATC TGGGCAGAAT ATGATCCTGC TGCTTGCGGG 
CGCATTCATT ATAAGGATAT GTACAGTTTA TTGCGAGTTA TCGACCCCCC TCTGGGCTTA 
GGCAAGAAAT GTCCTCATAG GGTGGCCTGC AAGAGACTGT TGCGGATGGA TCTGCCGGTT 
GCCGATGACA ACACAGTGCA CTTTAATTCT ACCCTTATGG CCTTAATCCG AACCGCTCTG 
GACATCAAGA TCGCCAAGGG AGGTGTAGAT AAGCACCAGA TGGATGCTGA ATTAAGGAAG 
GAGATGATGG CGATATGGCC TAATCTCTCT CAGAAGAACC TGGACCTGCT GGTGACTCCT 
CACAAGTCAG CGACAGACCT GACAGTAGGA AAGATCTACG CTGCCATGAT GATCATGGAG 
TATTATCGGC AAAGTAAAGC CAAACGGACA CAAGCTCTCC ATGATGAGCA GAATCGAACG 
CCATTAATGT TTCAGCGTCT GGAGCCGCCA TCTCCTAGCC AGGATGTGGG ACCAGGACTC 
ACTGGCCTTC CTGACACACA AATGCACCCT GCAAATCATC TGCCTGTTGA TGAAAGGATC 
CCAGAGAGCC AATCATGGGT GACAGCGCGA GCACAGGAGA TTTCTCAAAA AGCAGGCAAC 
TGGAGTCCAG AGGGCCAACA TCCAGATGAC ACCACAGACA ACAGAAGGCA GTCTCAGCCT 
ATCACAGATA ACAGTCCTAT GAAGCGCTCG GCTTCATCTT TGGGACACGG GCGTGCAGGA 
CGCTCCATGC GAGGTGAGGA TTATGCCATG GAGAGGGTTA TCCCAGAAGA GGGTCACAGG 
CATGGGCATC GCCACCGAGA CCGTAGTCAC CGGGCGTCAG AACGCTCCCT GAGCCGATAC 
ACTGATGCTG ACACAGGTCT TGGAACTGAC CTCAGCACAA CCACACAATC AGGAGACCTG 
CCATCCAAGG AGAGAGACCG GGGCCGAGCC AAAGACCGCA AGCACCATCA TCATCATCAT 
CACCACCACG GCTCTTTAGA TAAGGAGCAC TACGGTCATG AGAGAGACAG AGGAGAGTAC 
GGCCACAGAC AGTCCAGAGA GAGAGACCGC CGCTGGTCCC GCTCACCCAG TGAGGGCAGA 
GAGTGTCTGA CTCACAGACA GGGCAGTAGT TCAGTAAGCG GCAGTCCAGT CCCGTCCACC 
TCCGGGACCA GCACCCCCCG CCGTGGCCGC CGTCAGCTCC CCCAAACCCC TGCTACACCC 
CGGCCACATG TCACCTACTC ACCTGTGGTC CGTAAGCCCA TCAGCAGTAC CCCCCCTCCT 
GGCCAGCAGA GCCGACTCCC TACCCCGACC TCCCGGCGCT TCAGTCCGAC TGGCCCCGAG 
CCCCCGCTTC CCCCTCATCA CCACCCGTCA CCACCCCACC ACGGCTCTCC CCGCTCCGGC 
CGACATGCAC ACTGGGGCCC CGAGCCTGCA GAGAGCGTGG AGGGTGACGG CTTCTATGAC 
GACCAAGACT ATGAGTTCAA TCACCACGAG CCTCCATCCT ACGAGCAAAG CCCTGTGCAG 
GGCAACCCTC ACCCCCATTC CCCTAGGACT TCCCGCCACA ACACCCCTCC TCAGGGCCAA 
GCGCACCCAC GGCGCATGCC AAATGGCTAC CGTTCGTCTT CGCCCTCTCC ACACCACCAC 
GCGCCCGCTC ACCCTGGAAC TCATAAACCT CCTCATCCCA GAGGGCCCAG AAAGGGCCTC 
CATGAACCCT ACAGTGAGAC TGACGAGGAT GACTGGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_017354791.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017210280.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X2, mRNA.

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CloneID	ODa58247
Clone ID Related Accession (Same CDS sequence)	XM_017354791.1
Accession Version	XM_017354791.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGTGGCAGAA TCAGTTACAG GGATATGTAT GAGATGCTCA GGCACATGTC TCCTCCGTTA 
GGCCTGGGGA AGAAGTGCCC TGCACGGGTT GCCTACAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT 
GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC 
CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC 
ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT 
GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT 
GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT 
GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT 
CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA 
AGGCAGTCTC AGACAGTAGA GATGAGAGAG ATGGGGCGGG ACGGGTACTC GGACACAGAG 
CAATATCTGC CGATGGAAGG CCATGGCAGG GCTGCTTCCA TGCCTCGACT CCCAGCAGAC 
AACCAACAGC CTATCACAGA TAACAGTCCT ATGAAGCGCT CGGCTTCATC TTTGGGACAC 
GGGCGTGCAG GACGCTCCAT GCGAGGTGAG GATTATGCCA TGGAGAGGGT TATCCCAGAA 
GAGGGTCACA GGCATGGGCA TCGCCACCGA GACCGTAGTC ACCGGGCGTC AGAACGCTCC 
CTGAGCCGAT ACACTGATGC TGACACAGGT CTTGGAACTG ACCTCAGCAC AACCACACAA 
TCAGGAGACC TGCCATCCAA GGAGAGAGAC CGGGGCCGAG CCAAAGACCG CAAGCACCAT 
CATCATCATC ATCACCACCA CGGCTCTTTA GATAAGGAGC ACTACGGTCA TGAGAGAGAC 
AGAGGAGAGT ACGGCCACAG ACAGTCCAGA GAGAGAGACC GCCGCTGGTC CCGCTCACCC 
AGTGAGGGCA GAGAGTGTCT GACTCACAGA CAGGGCAGTA GTTCAGTAAG CGGCAGTCCA 
GTCCCGTCCA CCTCCGGGAC CAGCACCCCC CGCCGTGGCC GCCGTCAGCT CCCCCAAACC 
CCTGCTACAC CCCGGCCACA TGTCACCTAC TCACCTGTGG TCCGTAAGCC CATCAGCAGT 
ACCCCCCCTC CTGGCCAGCA GAGCCGACTC CCTACCCCGA CCTCCCGGCG CTTCAGTCCG 
ACTGGCCCCG AGCCCCCGCT TCCCCCTCAT CACCACCCGT CACCACCCCA CCACGGCTCT 
CCCCGCTCCG GCCGACATGC ACACTGGGGC CCCGAGCCTG CAGAGAGCGT GGAGGGTGAC 
GGCTTCTATG ACGACCAAGA CTATGAGTTC AATCACCACG AGCCTCCATC CTACGAGCAA 
AGCCCTGTGC AGGGCAACCC TCACCCCCAT TCCCCTAGGA CTTCCCGCCA CAACACCCCT 
CCTCAGGGCC AAGCGCACCC ACGGCGCATG CCAAATGGCT ACCGTTCGTC TTCGCCCTCT 
CCACACCACC ACGCGCCCGC TCACCCTGGA ACTCATAAAC CTCCTCATCC CAGAGGGCCC 
AGAAAGGGCC TCCATGAACC CTACAGTGAG ACTGACGAGG ATGACTGGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210280.1
CDS	1466..8539
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGGSGGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGMNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRISYRDMYEMLRHMSPPLGLGKKCPARVAYKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGEGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQNRTPLMFQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQTVEMREMGRDGYSDTEQYLPMEGHGRAASMPRLPADNQQPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_017354791.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354791.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGTGGCAGAA TCAGTTACAG GGATATGTAT GAGATGCTCA GGCACATGTC TCCTCCGTTA 
GGCCTGGGGA AGAAGTGCCC TGCACGGGTT GCCTACAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT 
GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC 
CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC 
ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT 
GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT 
GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT 
GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT 
CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA 
AGGCAGTCTC AGACAGTAGA GATGAGAGAG ATGGGGCGGG ACGGGTACTC GGACACAGAG 
CAATATCTGC CGATGGAAGG CCATGGCAGG GCTGCTTCCA TGCCTCGACT CCCAGCAGAC 
AACCAACAGC CTATCACAGA TAACAGTCCT ATGAAGCGCT CGGCTTCATC TTTGGGACAC 
GGGCGTGCAG GACGCTCCAT GCGAGGTGAG GATTATGCCA TGGAGAGGGT TATCCCAGAA 
GAGGGTCACA GGCATGGGCA TCGCCACCGA GACCGTAGTC ACCGGGCGTC AGAACGCTCC 
CTGAGCCGAT ACACTGATGC TGACACAGGT CTTGGAACTG ACCTCAGCAC AACCACACAA 
TCAGGAGACC TGCCATCCAA GGAGAGAGAC CGGGGCCGAG CCAAAGACCG CAAGCACCAT 
CATCATCATC ATCACCACCA CGGCTCTTTA GATAAGGAGC ACTACGGTCA TGAGAGAGAC 
AGAGGAGAGT ACGGCCACAG ACAGTCCAGA GAGAGAGACC GCCGCTGGTC CCGCTCACCC 
AGTGAGGGCA GAGAGTGTCT GACTCACAGA CAGGGCAGTA GTTCAGTAAG CGGCAGTCCA 
GTCCCGTCCA CCTCCGGGAC CAGCACCCCC CGCCGTGGCC GCCGTCAGCT CCCCCAAACC 
CCTGCTACAC CCCGGCCACA TGTCACCTAC TCACCTGTGG TCCGTAAGCC CATCAGCAGT 
ACCCCCCCTC CTGGCCAGCA GAGCCGACTC CCTACCCCGA CCTCCCGGCG CTTCAGTCCG 
ACTGGCCCCG AGCCCCCGCT TCCCCCTCAT CACCACCCGT CACCACCCCA CCACGGCTCT 
CCCCGCTCCG GCCGACATGC ACACTGGGGC CCCGAGCCTG CAGAGAGCGT GGAGGGTGAC 
GGCTTCTATG ACGACCAAGA CTATGAGTTC AATCACCACG AGCCTCCATC CTACGAGCAA 
AGCCCTGTGC AGGGCAACCC TCACCCCCAT TCCCCTAGGA CTTCCCGCCA CAACACCCCT 
CCTCAGGGCC AAGCGCACCC ACGGCGCATG CCAAATGGCT ACCGTTCGTC TTCGCCCTCT 
CCACACCACC ACGCGCCCGC TCACCCTGGA ACTCATAAAC CTCCTCATCC CAGAGGGCCC 
AGAAAGGGCC TCCATGAACC CTACAGTGAG ACTGACGAGG ATGACTGGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_017354792.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017210281.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X3, mRNA.

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CloneID	ODa58248
Clone ID Related Accession (Same CDS sequence)	XM_017354792.1
Accession Version	XM_017354792.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA ACAACTTCAT AAATCTGAGT 
TTTCTAAGGC TCTTCAGAGC TGCTCGTCTC ATTAAGTTGC TCAGGCAGGG TGAAACCATA 
CGCATCCTGC TCTGGACTTT TGTCCAGTCT TTCAAGGCTC TGCCGTATGT GTGCCTACTC 
ATCGCCATGC TGTTCTTCAT CTACGCCATC ATTGGCATGC AGCTGTTTGG GAACATTAAG 
ATCGAGGAGA ACAGTGACAG TGCCATTACC CAGCACAACA ACTTCAGGAC TTTTTTCCAG 
GCCCTAATGC TGCTCTTTAG GAGTGCTACA GGTGAGGCAT GGCATGACAT CATGCTGTCA 
TGTCTGGGAA AGAAGCCGTG TGATATTCTC TCTGATAACC CGAAGCCAGA ATGCGGGAGC 
GAGTTTGCCT ACCTCTACTT TGTCTCCTTC ATCTTCCTCT GTTCATTTCT GATGCTGAAT 
CTGTTCGTGG CTGTGATCAT GGATAATTTT GAATACCTGA CGCGTGACTC CTCTATCCTG 
GGACCCCATC ACCTGGACGA GTATGTGAGG ATCTGGGCAG AATATGATCC TGCTGCTTGC 
GGGCGCATTC ATTATAAGGA TATGTACAGT TTATTGCGAG TTATCGACCC CCCTCTGGGC 
TTAGGCAAGA AATGTCCTCA TAGGGTGGCC TGCAAGAGAC TGTTGCGGAT GGATCTGCCG 
GTTGCCGATG ACAACACAGT GCACTTTAAT TCTACCCTTA TGGCCTTAAT CCGAACCGCT 
CTGGACATCA AGATCGCCAA GGGTGGGGAA GGAGGTGTAG ATAAGCACCA GATGGATGCT 
GAATTAAGGA AGGAGATGAT GGCGATATGG CCTAATCTCT CTCAGAAGAA CCTGGACCTG 
CTGGTGACTC CTCACAAGTC AGCGACAGAC CTGACAGTAG GAAAGATCTA CGCTGCCATG 
ATGATCATGG AGTATTATCG GCAAAGTAAA GCCAAACGGA CACAAGCTCT CCATGATGAG 
CAGAATCGAA CGCCATTAAT GTTTCAGCGT CTGGAGCCGC CATCTCCTAG CCAGGATGTG 
GGACCAGGAC TCACTGGCCT TCCTGACACA CAAATGCACC CTGCAAATCA TCTGCCTGTT 
GATGAAAGGA TCCCAGAGAG CCAATCATGG GTGACAGCGC GAGCACAGGA GATTTCTCAA 
AAAGCAGGCA ACTGGAGTCC AGAGGGCCAA CATCCAGATG ACACCACAGA CAACAGAAGG 
CAGTCTCAGA CAGTAGAGAT GAGAGAGATG GGGCGGGACG GGTACTCGGA CACAGAGCAA 
TATCTGCCGA TGGAAGGCCA TGGCAGGGCT GCTTCCATGC CTCGACTCCC AGCAGACAAC 
CAACAGCCTA TCACAGATAA CAGTCCTATG AAGCGCTCGG CTTCATCTTT GGGACACGGG 
CGTGCAGGAC GCTCCATGCG AGGTGAGGAT TATGCCATGG AGAGGGTTAT CCCAGAAGAG 
GGTCACAGGC ATGGGCATCG CCACCGAGAC CGTAGTCACC GGGCGTCAGA ACGCTCCCTG 
AGCCGATACA CTGATGCTGA CACAGGTCTT GGAACTGACC TCAGCACAAC CACACAATCA 
GGAGACCTGC CATCCAAGGA GAGAGACCGG GGCCGAGCCA AAGACCGCAA GCACCATCAT 
CATCATCATC ACCACCACGG CTCTTTAGAT AAGGAGCACT ACGGTCATGA GAGAGACAGA 
GGAGAGTACG GCCACAGACA GTCCAGAGAG AGAGACCGCC GCTGGTCCCG CTCACCCAGT 
GAGGGCAGAG AGTGTCTGAC TCACAGACAG GGCAGTAGTT CAGTAAGCGG CAGTCCAGTC 
CCGTCCACCT CCGGGACCAG CACCCCCCGC CGTGGCCGCC GTCAGCTCCC CCAAACCCCT 
GCTACACCCC GGCCACATGT CACCTACTCA CCTGTGGTCC GTAAGCCCAT CAGCAGTACC 
CCCCCTCCTG GCCAGCAGAG CCGACTCCCT ACCCCGACCT CCCGGCGCTT CAGTCCGACT 
GGCCCCGAGC CCCCGCTTCC CCCTCATCAC CACCCGTCAC CACCCCACCA CGGCTCTCCC 
CGCTCCGGCC GACATGCACA CTGGGGCCCC GAGCCTGCAG AGAGCGTGGA GGGTGACGGC 
TTCTATGACG ACCAAGACTA TGAGTTCAAT CACCACGAGC CTCCATCCTA CGAGCAAAGC 
CCTGTGCAGG GCAACCCTCA CCCCCATTCC CCTAGGACTT CCCGCCACAA CACCCCTCCT 
CAGGGCCAAG CGCACCCACG GCGCATGCCA AATGGCTACC GTTCGTCTTC GCCCTCTCCA 
CACCACCACG CGCCCGCTCA CCCTGGAACT CATAAACCTC CTCATCCCAG AGGGCCCAGA 
AAGGGCCTCC ATGAACCCTA CAGTGAGACT GACGAGGATG ACTGGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210281.1
CDS	1466..8536
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGGSGGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGEGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQNRTPLMFQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQTVEMREMGRDGYSDTEQYLPMEGHGRAASMPRLPADNQQPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_017354792.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354792.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA ACAACTTCAT AAATCTGAGT 
TTTCTAAGGC TCTTCAGAGC TGCTCGTCTC ATTAAGTTGC TCAGGCAGGG TGAAACCATA 
CGCATCCTGC TCTGGACTTT TGTCCAGTCT TTCAAGGCTC TGCCGTATGT GTGCCTACTC 
ATCGCCATGC TGTTCTTCAT CTACGCCATC ATTGGCATGC AGCTGTTTGG GAACATTAAG 
ATCGAGGAGA ACAGTGACAG TGCCATTACC CAGCACAACA ACTTCAGGAC TTTTTTCCAG 
GCCCTAATGC TGCTCTTTAG GAGTGCTACA GGTGAGGCAT GGCATGACAT CATGCTGTCA 
TGTCTGGGAA AGAAGCCGTG TGATATTCTC TCTGATAACC CGAAGCCAGA ATGCGGGAGC 
GAGTTTGCCT ACCTCTACTT TGTCTCCTTC ATCTTCCTCT GTTCATTTCT GATGCTGAAT 
CTGTTCGTGG CTGTGATCAT GGATAATTTT GAATACCTGA CGCGTGACTC CTCTATCCTG 
GGACCCCATC ACCTGGACGA GTATGTGAGG ATCTGGGCAG AATATGATCC TGCTGCTTGC 
GGGCGCATTC ATTATAAGGA TATGTACAGT TTATTGCGAG TTATCGACCC CCCTCTGGGC 
TTAGGCAAGA AATGTCCTCA TAGGGTGGCC TGCAAGAGAC TGTTGCGGAT GGATCTGCCG 
GTTGCCGATG ACAACACAGT GCACTTTAAT TCTACCCTTA TGGCCTTAAT CCGAACCGCT 
CTGGACATCA AGATCGCCAA GGGTGGGGAA GGAGGTGTAG ATAAGCACCA GATGGATGCT 
GAATTAAGGA AGGAGATGAT GGCGATATGG CCTAATCTCT CTCAGAAGAA CCTGGACCTG 
CTGGTGACTC CTCACAAGTC AGCGACAGAC CTGACAGTAG GAAAGATCTA CGCTGCCATG 
ATGATCATGG AGTATTATCG GCAAAGTAAA GCCAAACGGA CACAAGCTCT CCATGATGAG 
CAGAATCGAA CGCCATTAAT GTTTCAGCGT CTGGAGCCGC CATCTCCTAG CCAGGATGTG 
GGACCAGGAC TCACTGGCCT TCCTGACACA CAAATGCACC CTGCAAATCA TCTGCCTGTT 
GATGAAAGGA TCCCAGAGAG CCAATCATGG GTGACAGCGC GAGCACAGGA GATTTCTCAA 
AAAGCAGGCA ACTGGAGTCC AGAGGGCCAA CATCCAGATG ACACCACAGA CAACAGAAGG 
CAGTCTCAGA CAGTAGAGAT GAGAGAGATG GGGCGGGACG GGTACTCGGA CACAGAGCAA 
TATCTGCCGA TGGAAGGCCA TGGCAGGGCT GCTTCCATGC CTCGACTCCC AGCAGACAAC 
CAACAGCCTA TCACAGATAA CAGTCCTATG AAGCGCTCGG CTTCATCTTT GGGACACGGG 
CGTGCAGGAC GCTCCATGCG AGGTGAGGAT TATGCCATGG AGAGGGTTAT CCCAGAAGAG 
GGTCACAGGC ATGGGCATCG CCACCGAGAC CGTAGTCACC GGGCGTCAGA ACGCTCCCTG 
AGCCGATACA CTGATGCTGA CACAGGTCTT GGAACTGACC TCAGCACAAC CACACAATCA 
GGAGACCTGC CATCCAAGGA GAGAGACCGG GGCCGAGCCA AAGACCGCAA GCACCATCAT 
CATCATCATC ACCACCACGG CTCTTTAGAT AAGGAGCACT ACGGTCATGA GAGAGACAGA 
GGAGAGTACG GCCACAGACA GTCCAGAGAG AGAGACCGCC GCTGGTCCCG CTCACCCAGT 
GAGGGCAGAG AGTGTCTGAC TCACAGACAG GGCAGTAGTT CAGTAAGCGG CAGTCCAGTC 
CCGTCCACCT CCGGGACCAG CACCCCCCGC CGTGGCCGCC GTCAGCTCCC CCAAACCCCT 
GCTACACCCC GGCCACATGT CACCTACTCA CCTGTGGTCC GTAAGCCCAT CAGCAGTACC 
CCCCCTCCTG GCCAGCAGAG CCGACTCCCT ACCCCGACCT CCCGGCGCTT CAGTCCGACT 
GGCCCCGAGC CCCCGCTTCC CCCTCATCAC CACCCGTCAC CACCCCACCA CGGCTCTCCC 
CGCTCCGGCC GACATGCACA CTGGGGCCCC GAGCCTGCAG AGAGCGTGGA GGGTGACGGC 
TTCTATGACG ACCAAGACTA TGAGTTCAAT CACCACGAGC CTCCATCCTA CGAGCAAAGC 
CCTGTGCAGG GCAACCCTCA CCCCCATTCC CCTAGGACTT CCCGCCACAA CACCCCTCCT 
CAGGGCCAAG CGCACCCACG GCGCATGCCA AATGGCTACC GTTCGTCTTC GCCCTCTCCA 
CACCACCACG CGCCCGCTCA CCCTGGAACT CATAAACCTC CTCATCCCAG AGGGCCCAGA 
AAGGGCCTCC ATGAACCCTA CAGTGAGACT GACGAGGATG ACTGGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_017354793.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017210282.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X4, mRNA.

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CloneID	ODa58249
Clone ID Related Accession (Same CDS sequence)	XM_017354793.1
Accession Version	XM_017354793.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG 
GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT 
GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC 
CTGCTGGTGA CTCCTCACAA GTCGACAGAC CTGACAGTAG GAAAGATCTA CGCTGCCATG 
ATGATCATGG AGTATTATCG GCAAAGTAAA GCCAAACGGA CACAAGCTCT CCATGATGAG 
CAGAATCGAA CGCCATTAAT GTTTCAGCGT CTGGAGCCGC CATCTCCTAG CCAGGATGTG 
GGACCAGGAC TCACTGGCCT TCCTGACACA CAAATGCACC CTGCAAATCA TCTGCCTGTT 
GATGAAAGGA TCCCAGAGAG CCAATCATGG GTGACAGCGC GAGCACAGGA GATTTCTCAA 
AAAGCAGGCA ACTGGAGTCC AGAGGGCCAA CATCCAGATG ACACCACAGA CAACAGAAGG 
CAGTCTCAGA CAGTAGAGAT GAGAGAGATG GGGCGGGACG GGTACTCGGA CACAGAGCAA 
TATCTGCCGA TGGAAGGCCA TGGCAGGGCT GCTTCCATGC CTCGACTCCC AGCAGACAAC 
CAACAGCCTA TCACAGATAA CAGTCCTATG AAGCGCTCGG CTTCATCTTT GGGACACGGG 
CGTGCAGGAC GCTCCATGCG AGGTGAGGAT TATGCCATGG AGAGGGTTAT CCCAGAAGAG 
GGTCACAGGC ATGGGCATCG CCACCGAGAC CGTAGTCACC GGGCGTCAGA ACGCTCCCTG 
AGCCGATACA CTGATGCTGA CACAGGTCTT GGAACTGACC TCAGCACAAC CACACAATCA 
GGAGACCTGC CATCCAAGGA GAGAGACCGG GGCCGAGCCA AAGACCGCAA GCACCATCAT 
CATCATCATC ACCACCACGG CTCTTTAGAT AAGGAGCACT ACGGTCATGA GAGAGACAGA 
GGAGAGTACG GCCACAGACA GTCCAGAGAG AGAGACCGCC GCTGGTCCCG CTCACCCAGT 
GAGGGCAGAG AGTGTCTGAC TCACAGACAG GGCAGTAGTT CAGTAAGCGG CAGTCCAGTC 
CCGTCCACCT CCGGGACCAG CACCCCCCGC CGTGGCCGCC GTCAGCTCCC CCAAACCCCT 
GCTACACCCC GGCCACATGT CACCTACTCA CCTGTGGTCC GTAAGCCCAT CAGCAGTACC 
CCCCCTCCTG GCCAGCAGAG CCGACTCCCT ACCCCGACCT CCCGGCGCTT CAGTCCGACT 
GGCCCCGAGC CCCCGCTTCC CCCTCATCAC CACCCGTCAC CACCCCACCA CGGCTCTCCC 
CGCTCCGGCC GACATGCACA CTGGGGCCCC GAGCCTGCAG AGAGCGTGGA GGGTGACGGC 
TTCTATGACG ACCAAGACTA TGAGTTCAAT CACCACGAGC CTCCATCCTA CGAGCAAAGC 
CCTGTGCAGG GCAACCCTCA CCCCCATTCC CCTAGGACTT CCCGCCACAA CACCCCTCCT 
CAGGGCCAAG CGCACCCACG GCGCATGCCA AATGGCTACC GTTCGTCTTC GCCCTCTCCA 
CACCACCACG CGCCCGCTCA CCCTGGAACT CATAAACCTC CTCATCCCAG AGGGCCCAGA 
AAGGGCCTCC ATGAACCCTA CAGTGAGACT GACGAGGATG ACTGGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210282.1
CDS	1467..8537
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGGSGGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGMNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGEGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQNRTPLMFQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQTVEMREMGRDGYSDTEQYLPMEGHGRAASMPRLPADNQQPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_017354793.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354793.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG 
GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT 
GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC 
CTGCTGGTGA CTCCTCACAA GTCGACAGAC CTGACAGTAG GAAAGATCTA CGCTGCCATG 
ATGATCATGG AGTATTATCG GCAAAGTAAA GCCAAACGGA CACAAGCTCT CCATGATGAG 
CAGAATCGAA CGCCATTAAT GTTTCAGCGT CTGGAGCCGC CATCTCCTAG CCAGGATGTG 
GGACCAGGAC TCACTGGCCT TCCTGACACA CAAATGCACC CTGCAAATCA TCTGCCTGTT 
GATGAAAGGA TCCCAGAGAG CCAATCATGG GTGACAGCGC GAGCACAGGA GATTTCTCAA 
AAAGCAGGCA ACTGGAGTCC AGAGGGCCAA CATCCAGATG ACACCACAGA CAACAGAAGG 
CAGTCTCAGA CAGTAGAGAT GAGAGAGATG GGGCGGGACG GGTACTCGGA CACAGAGCAA 
TATCTGCCGA TGGAAGGCCA TGGCAGGGCT GCTTCCATGC CTCGACTCCC AGCAGACAAC 
CAACAGCCTA TCACAGATAA CAGTCCTATG AAGCGCTCGG CTTCATCTTT GGGACACGGG 
CGTGCAGGAC GCTCCATGCG AGGTGAGGAT TATGCCATGG AGAGGGTTAT CCCAGAAGAG 
GGTCACAGGC ATGGGCATCG CCACCGAGAC CGTAGTCACC GGGCGTCAGA ACGCTCCCTG 
AGCCGATACA CTGATGCTGA CACAGGTCTT GGAACTGACC TCAGCACAAC CACACAATCA 
GGAGACCTGC CATCCAAGGA GAGAGACCGG GGCCGAGCCA AAGACCGCAA GCACCATCAT 
CATCATCATC ACCACCACGG CTCTTTAGAT AAGGAGCACT ACGGTCATGA GAGAGACAGA 
GGAGAGTACG GCCACAGACA GTCCAGAGAG AGAGACCGCC GCTGGTCCCG CTCACCCAGT 
GAGGGCAGAG AGTGTCTGAC TCACAGACAG GGCAGTAGTT CAGTAAGCGG CAGTCCAGTC 
CCGTCCACCT CCGGGACCAG CACCCCCCGC CGTGGCCGCC GTCAGCTCCC CCAAACCCCT 
GCTACACCCC GGCCACATGT CACCTACTCA CCTGTGGTCC GTAAGCCCAT CAGCAGTACC 
CCCCCTCCTG GCCAGCAGAG CCGACTCCCT ACCCCGACCT CCCGGCGCTT CAGTCCGACT 
GGCCCCGAGC CCCCGCTTCC CCCTCATCAC CACCCGTCAC CACCCCACCA CGGCTCTCCC 
CGCTCCGGCC GACATGCACA CTGGGGCCCC GAGCCTGCAG AGAGCGTGGA GGGTGACGGC 
TTCTATGACG ACCAAGACTA TGAGTTCAAT CACCACGAGC CTCCATCCTA CGAGCAAAGC 
CCTGTGCAGG GCAACCCTCA CCCCCATTCC CCTAGGACTT CCCGCCACAA CACCCCTCCT 
CAGGGCCAAG CGCACCCACG GCGCATGCCA AATGGCTACC GTTCGTCTTC GCCCTCTCCA 
CACCACCACG CGCCCGCTCA CCCTGGAACT CATAAACCTC CTCATCCCAG AGGGCCCAGA 
AAGGGCCTCC ATGAACCCTA CAGTGAGACT GACGAGGATG ACTGGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_017354794.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017210283.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X15, mRNA.

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CloneID	ODa58250
Clone ID Related Accession (Same CDS sequence)	XM_017354794.2 , XM_017354794.1
Accession Version	XM_017354794.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017354794.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG 
GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT 
GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC 
CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC 
ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT 
GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT 
GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT 
GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT 
CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA 
AGGCAGTCTC AGACAGTAGA GATGAGAGAG ATGGGGCGGG ACGGGTACTC GGACACAGAG 
CAATATCTGC CGATGGAAGG CCATGGCAGG GCTGCTTCCA TGCCTCGACT CCCAGCAGAC 
AACCAACCTA TCACAGATAA CAGTCCTATG AAGCGCTCGG CTTCATCTTT GGGACACGGG 
CGTGCAGGAC GCTCCATGCG AGGTGAGGAT TATGCCATGG AGAGGGTTAT CCCAGAAGAG 
GGTCACAGGC ATGGGCATCG CCACCGAGAC CGTAGTCACC GGGCGTCAGA ACGCTCCCTG 
AGCCGATACA CTGATGCTGA CACAGGTCTT GGAACTGACC TCAGCACAAC CACACAATCA 
GGAGACCTGC CATCCAAGGA GAGAGACCGG GGCCGAGCCA AAGACCGCAA GCACCATCAT 
CATCATCATC ACCACCACGG CTCTTTAGAT AAGGAGCACT ACGGTCATGA GAGAGACAGA 
GGAGAGTACG GCCACAGACA GTCCAGAGAG AGAGACCGCC GCTGGTCCCG CTCACCCAGT 
GAGGGCAGAG AGTGTCTGAC TCACAGACAG GGCAGTAGTT CAGTAAGCGG CAGTCCAGTC 
CCGTCCACCT CCGGGACCAG CACCCCCCGC CGTGGCCGCC GTCAGCTCCC CCAAACCCCT 
GCTACACCCC GGCCACATGT CACCTACTCA CCTGTGGTCC GTAAGCCCAT CAGCAGTACC 
CCCCCTCCTG GCCAGCAGAG CCGACTCCCT ACCCCGACCT CCCGGCGCTT CAGTCCGACT 
GGCCCCGAGC CCCCGCTTCC CCCTCATCAC CACCCGTCAC CACCCCACCA CGGCTCTCCC 
CGCTCCGGCC GACATGCACA CTGGGGCCCC GAGCCTGCAG AGAGCGTGGA GGGTGACGGC 
TTCTATGACG ACCAAGACTA TGAGTTCAAT CACCACGAGC CTCCATCCTA CGAGCAAAGC 
CCTGTGCAGG GCAACCCTCA CCCCCATTCC CCTAGGACTT CCCGCCACAA CACCCCTCCT 
CAGGGCCAAG CGCACCCACG GCGCATGCCA AATGGCTACC GTTCGTCTTC GCCCTCTCCA 
CACCACCACG CGCCCGCTCA CCCTGGAACT CATAAACCTC CTCATCCCAG AGGGCCCAGA 
AAGGGCCTCC ATGAACCCTA CAGTGAGACT GACGAGGATG ACTGGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210283.1
CDS	1506..8576
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGGSGGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGMNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGEGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQNRTPLMFQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQTVEMREMGRDGYSDTEQYLPMEGHGRAASMPRLPADNQPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_017354794.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354794.2

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG 
GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT 
GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC 
CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC 
ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT 
GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT 
GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT 
GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT 
CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA 
AGGCAGTCTC AGACAGTAGA GATGAGAGAG ATGGGGCGGG ACGGGTACTC GGACACAGAG 
CAATATCTGC CGATGGAAGG CCATGGCAGG GCTGCTTCCA TGCCTCGACT CCCAGCAGAC 
AACCAACCTA TCACAGATAA CAGTCCTATG AAGCGCTCGG CTTCATCTTT GGGACACGGG 
CGTGCAGGAC GCTCCATGCG AGGTGAGGAT TATGCCATGG AGAGGGTTAT CCCAGAAGAG 
GGTCACAGGC ATGGGCATCG CCACCGAGAC CGTAGTCACC GGGCGTCAGA ACGCTCCCTG 
AGCCGATACA CTGATGCTGA CACAGGTCTT GGAACTGACC TCAGCACAAC CACACAATCA 
GGAGACCTGC CATCCAAGGA GAGAGACCGG GGCCGAGCCA AAGACCGCAA GCACCATCAT 
CATCATCATC ACCACCACGG CTCTTTAGAT AAGGAGCACT ACGGTCATGA GAGAGACAGA 
GGAGAGTACG GCCACAGACA GTCCAGAGAG AGAGACCGCC GCTGGTCCCG CTCACCCAGT 
GAGGGCAGAG AGTGTCTGAC TCACAGACAG GGCAGTAGTT CAGTAAGCGG CAGTCCAGTC 
CCGTCCACCT CCGGGACCAG CACCCCCCGC CGTGGCCGCC GTCAGCTCCC CCAAACCCCT 
GCTACACCCC GGCCACATGT CACCTACTCA CCTGTGGTCC GTAAGCCCAT CAGCAGTACC 
CCCCCTCCTG GCCAGCAGAG CCGACTCCCT ACCCCGACCT CCCGGCGCTT CAGTCCGACT 
GGCCCCGAGC CCCCGCTTCC CCCTCATCAC CACCCGTCAC CACCCCACCA CGGCTCTCCC 
CGCTCCGGCC GACATGCACA CTGGGGCCCC GAGCCTGCAG AGAGCGTGGA GGGTGACGGC 
TTCTATGACG ACCAAGACTA TGAGTTCAAT CACCACGAGC CTCCATCCTA CGAGCAAAGC 
CCTGTGCAGG GCAACCCTCA CCCCCATTCC CCTAGGACTT CCCGCCACAA CACCCCTCCT 
CAGGGCCAAG CGCACCCACG GCGCATGCCA AATGGCTACC GTTCGTCTTC GCCCTCTCCA 
CACCACCACG CGCCCGCTCA CCCTGGAACT CATAAACCTC CTCATCCCAG AGGGCCCAGA 
AAGGGCCTCC ATGAACCCTA CAGTGAGACT GACGAGGATG ACTGGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_017354794.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017210283.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X5, mRNA.

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CloneID	ODa58250
Clone ID Related Accession (Same CDS sequence)	XM_017354794.2 , XM_017354794.1
Accession Version	XM_017354794.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG 
GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT 
GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC 
CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC 
ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT 
GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT 
GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT 
GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT 
CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA 
AGGCAGTCTC AGACAGTAGA GATGAGAGAG ATGGGGCGGG ACGGGTACTC GGACACAGAG 
CAATATCTGC CGATGGAAGG CCATGGCAGG GCTGCTTCCA TGCCTCGACT CCCAGCAGAC 
AACCAACCTA TCACAGATAA CAGTCCTATG AAGCGCTCGG CTTCATCTTT GGGACACGGG 
CGTGCAGGAC GCTCCATGCG AGGTGAGGAT TATGCCATGG AGAGGGTTAT CCCAGAAGAG 
GGTCACAGGC ATGGGCATCG CCACCGAGAC CGTAGTCACC GGGCGTCAGA ACGCTCCCTG 
AGCCGATACA CTGATGCTGA CACAGGTCTT GGAACTGACC TCAGCACAAC CACACAATCA 
GGAGACCTGC CATCCAAGGA GAGAGACCGG GGCCGAGCCA AAGACCGCAA GCACCATCAT 
CATCATCATC ACCACCACGG CTCTTTAGAT AAGGAGCACT ACGGTCATGA GAGAGACAGA 
GGAGAGTACG GCCACAGACA GTCCAGAGAG AGAGACCGCC GCTGGTCCCG CTCACCCAGT 
GAGGGCAGAG AGTGTCTGAC TCACAGACAG GGCAGTAGTT CAGTAAGCGG CAGTCCAGTC 
CCGTCCACCT CCGGGACCAG CACCCCCCGC CGTGGCCGCC GTCAGCTCCC CCAAACCCCT 
GCTACACCCC GGCCACATGT CACCTACTCA CCTGTGGTCC GTAAGCCCAT CAGCAGTACC 
CCCCCTCCTG GCCAGCAGAG CCGACTCCCT ACCCCGACCT CCCGGCGCTT CAGTCCGACT 
GGCCCCGAGC CCCCGCTTCC CCCTCATCAC CACCCGTCAC CACCCCACCA CGGCTCTCCC 
CGCTCCGGCC GACATGCACA CTGGGGCCCC GAGCCTGCAG AGAGCGTGGA GGGTGACGGC 
TTCTATGACG ACCAAGACTA TGAGTTCAAT CACCACGAGC CTCCATCCTA CGAGCAAAGC 
CCTGTGCAGG GCAACCCTCA CCCCCATTCC CCTAGGACTT CCCGCCACAA CACCCCTCCT 
CAGGGCCAAG CGCACCCACG GCGCATGCCA AATGGCTACC GTTCGTCTTC GCCCTCTCCA 
CACCACCACG CGCCCGCTCA CCCTGGAACT CATAAACCTC CTCATCCCAG AGGGCCCAGA 
AAGGGCCTCC ATGAACCCTA CAGTGAGACT GACGAGGATG ACTGGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210283.1
CDS	1467..8537
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGGSGGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGMNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGEGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQNRTPLMFQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQTVEMREMGRDGYSDTEQYLPMEGHGRAASMPRLPADNQPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_017354794.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354794.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG 
GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT 
GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC 
CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC 
ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT 
GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT 
GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT 
GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT 
CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA 
AGGCAGTCTC AGACAGTAGA GATGAGAGAG ATGGGGCGGG ACGGGTACTC GGACACAGAG 
CAATATCTGC CGATGGAAGG CCATGGCAGG GCTGCTTCCA TGCCTCGACT CCCAGCAGAC 
AACCAACCTA TCACAGATAA CAGTCCTATG AAGCGCTCGG CTTCATCTTT GGGACACGGG 
CGTGCAGGAC GCTCCATGCG AGGTGAGGAT TATGCCATGG AGAGGGTTAT CCCAGAAGAG 
GGTCACAGGC ATGGGCATCG CCACCGAGAC CGTAGTCACC GGGCGTCAGA ACGCTCCCTG 
AGCCGATACA CTGATGCTGA CACAGGTCTT GGAACTGACC TCAGCACAAC CACACAATCA 
GGAGACCTGC CATCCAAGGA GAGAGACCGG GGCCGAGCCA AAGACCGCAA GCACCATCAT 
CATCATCATC ACCACCACGG CTCTTTAGAT AAGGAGCACT ACGGTCATGA GAGAGACAGA 
GGAGAGTACG GCCACAGACA GTCCAGAGAG AGAGACCGCC GCTGGTCCCG CTCACCCAGT 
GAGGGCAGAG AGTGTCTGAC TCACAGACAG GGCAGTAGTT CAGTAAGCGG CAGTCCAGTC 
CCGTCCACCT CCGGGACCAG CACCCCCCGC CGTGGCCGCC GTCAGCTCCC CCAAACCCCT 
GCTACACCCC GGCCACATGT CACCTACTCA CCTGTGGTCC GTAAGCCCAT CAGCAGTACC 
CCCCCTCCTG GCCAGCAGAG CCGACTCCCT ACCCCGACCT CCCGGCGCTT CAGTCCGACT 
GGCCCCGAGC CCCCGCTTCC CCCTCATCAC CACCCGTCAC CACCCCACCA CGGCTCTCCC 
CGCTCCGGCC GACATGCACA CTGGGGCCCC GAGCCTGCAG AGAGCGTGGA GGGTGACGGC 
TTCTATGACG ACCAAGACTA TGAGTTCAAT CACCACGAGC CTCCATCCTA CGAGCAAAGC 
CCTGTGCAGG GCAACCCTCA CCCCCATTCC CCTAGGACTT CCCGCCACAA CACCCCTCCT 
CAGGGCCAAG CGCACCCACG GCGCATGCCA AATGGCTACC GTTCGTCTTC GCCCTCTCCA 
CACCACCACG CGCCCGCTCA CCCTGGAACT CATAAACCTC CTCATCCCAG AGGGCCCAGA 
AAGGGCCTCC ATGAACCCTA CAGTGAGACT GACGAGGATG ACTGGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_017354795.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017210284.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X6, mRNA.

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CloneID	ODa58251
Clone ID Related Accession (Same CDS sequence)	XM_017354795.1
Accession Version	XM_017354795.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG 
GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGAGGT GTAGATAAGC ACCAGATGGA TGCTGAATTA 
AGGAAGGAGA TGATGGCGAT ATGGCCTAAT CTCTCTCAGA AGAACCTGGA CCTGCTGGTG 
ACTCCTCACA AGTCAGCGAC AGACCTGACA GTAGGAAAGA TCTACGCTGC CATGATGATC 
ATGGAGTATT ATCGGCAAAG TAAAGCCAAA CGGACACAAG CTCTCCATGA TGAGCAGAAT 
CGAACGCCAT TAATGTTTCA GCGTCTGGAG CCGCCATCTC CTAGCCAGGA TGTGGGACCA 
GGACTCACTG GCCTTCCTGA CACACAAATG CACCCTGCAA ATCATCTGCC TGTTGATGAA 
AGGATCCCAG AGAGCCAATC ATGGGTGACA GCGCGAGCAC AGGAGATTTC TCAAAAAGCA 
GGCAACTGGA GTCCAGAGGG CCAACATCCA GATGACACCA CAGACAACAG AAGGCAGTCT 
CAGACAGTAG AGATGAGAGA GATGGGGCGG GACGGGTACT CGGACACAGA GCAATATCTG 
CCGATGGAAG GCCATGGCAG GGCTGCTTCC ATGCCTCGAC TCCCAGCAGA CAACCAACAG 
CCTATCACAG ATAACAGTCC TATGAAGCGC TCGGCTTCAT CTTTGGGACA CGGGCGTGCA 
GGACGCTCCA TGCGAGGTGA GGATTATGCC ATGGAGAGGG TTATCCCAGA AGAGGGTCAC 
AGGCATGGGC ATCGCCACCG AGACCGTAGT CACCGGGCGT CAGAACGCTC CCTGAGCCGA 
TACACTGATG CTGACACAGG TCTTGGAACT GACCTCAGCA CAACCACACA ATCAGGAGAC 
CTGCCATCCA AGGAGAGAGA CCGGGGCCGA GCCAAAGACC GCAAGCACCA TCATCATCAT 
CATCACCACC ACGGCTCTTT AGATAAGGAG CACTACGGTC ATGAGAGAGA CAGAGGAGAG 
TACGGCCACA GACAGTCCAG AGAGAGAGAC CGCCGCTGGT CCCGCTCACC CAGTGAGGGC 
AGAGAGTGTC TGACTCACAG ACAGGGCAGT AGTTCAGTAA GCGGCAGTCC AGTCCCGTCC 
ACCTCCGGGA CCAGCACCCC CCGCCGTGGC CGCCGTCAGC TCCCCCAAAC CCCTGCTACA 
CCCCGGCCAC ATGTCACCTA CTCACCTGTG GTCCGTAAGC CCATCAGCAG TACCCCCCCT 
CCTGGCCAGC AGAGCCGACT CCCTACCCCG ACCTCCCGGC GCTTCAGTCC GACTGGCCCC 
GAGCCCCCGC TTCCCCCTCA TCACCACCCG TCACCACCCC ACCACGGCTC TCCCCGCTCC 
GGCCGACATG CACACTGGGG CCCCGAGCCT GCAGAGAGCG TGGAGGGTGA CGGCTTCTAT 
GACGACCAAG ACTATGAGTT CAATCACCAC GAGCCTCCAT CCTACGAGCA AAGCCCTGTG 
CAGGGCAACC CTCACCCCCA TTCCCCTAGG ACTTCCCGCC ACAACACCCC TCCTCAGGGC 
CAAGCGCACC CACGGCGCAT GCCAAATGGC TACCGTTCGT CTTCGCCCTC TCCACACCAC 
CACGCGCCCG CTCACCCTGG AACTCATAAA CCTCCTCATC CCAGAGGGCC CAGAAAGGGC 
CTCCATGAAC CCTACAGTGA GACTGACGAG GATGACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210284.1
CDS	1467..8531
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGGSGGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGMNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQNRTPLMFQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQTVEMREMGRDGYSDTEQYLPMEGHGRAASMPRLPADNQQPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_017354795.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354795.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG 
GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGAGGT GTAGATAAGC ACCAGATGGA TGCTGAATTA 
AGGAAGGAGA TGATGGCGAT ATGGCCTAAT CTCTCTCAGA AGAACCTGGA CCTGCTGGTG 
ACTCCTCACA AGTCAGCGAC AGACCTGACA GTAGGAAAGA TCTACGCTGC CATGATGATC 
ATGGAGTATT ATCGGCAAAG TAAAGCCAAA CGGACACAAG CTCTCCATGA TGAGCAGAAT 
CGAACGCCAT TAATGTTTCA GCGTCTGGAG CCGCCATCTC CTAGCCAGGA TGTGGGACCA 
GGACTCACTG GCCTTCCTGA CACACAAATG CACCCTGCAA ATCATCTGCC TGTTGATGAA 
AGGATCCCAG AGAGCCAATC ATGGGTGACA GCGCGAGCAC AGGAGATTTC TCAAAAAGCA 
GGCAACTGGA GTCCAGAGGG CCAACATCCA GATGACACCA CAGACAACAG AAGGCAGTCT 
CAGACAGTAG AGATGAGAGA GATGGGGCGG GACGGGTACT CGGACACAGA GCAATATCTG 
CCGATGGAAG GCCATGGCAG GGCTGCTTCC ATGCCTCGAC TCCCAGCAGA CAACCAACAG 
CCTATCACAG ATAACAGTCC TATGAAGCGC TCGGCTTCAT CTTTGGGACA CGGGCGTGCA 
GGACGCTCCA TGCGAGGTGA GGATTATGCC ATGGAGAGGG TTATCCCAGA AGAGGGTCAC 
AGGCATGGGC ATCGCCACCG AGACCGTAGT CACCGGGCGT CAGAACGCTC CCTGAGCCGA 
TACACTGATG CTGACACAGG TCTTGGAACT GACCTCAGCA CAACCACACA ATCAGGAGAC 
CTGCCATCCA AGGAGAGAGA CCGGGGCCGA GCCAAAGACC GCAAGCACCA TCATCATCAT 
CATCACCACC ACGGCTCTTT AGATAAGGAG CACTACGGTC ATGAGAGAGA CAGAGGAGAG 
TACGGCCACA GACAGTCCAG AGAGAGAGAC CGCCGCTGGT CCCGCTCACC CAGTGAGGGC 
AGAGAGTGTC TGACTCACAG ACAGGGCAGT AGTTCAGTAA GCGGCAGTCC AGTCCCGTCC 
ACCTCCGGGA CCAGCACCCC CCGCCGTGGC CGCCGTCAGC TCCCCCAAAC CCCTGCTACA 
CCCCGGCCAC ATGTCACCTA CTCACCTGTG GTCCGTAAGC CCATCAGCAG TACCCCCCCT 
CCTGGCCAGC AGAGCCGACT CCCTACCCCG ACCTCCCGGC GCTTCAGTCC GACTGGCCCC 
GAGCCCCCGC TTCCCCCTCA TCACCACCCG TCACCACCCC ACCACGGCTC TCCCCGCTCC 
GGCCGACATG CACACTGGGG CCCCGAGCCT GCAGAGAGCG TGGAGGGTGA CGGCTTCTAT 
GACGACCAAG ACTATGAGTT CAATCACCAC GAGCCTCCAT CCTACGAGCA AAGCCCTGTG 
CAGGGCAACC CTCACCCCCA TTCCCCTAGG ACTTCCCGCC ACAACACCCC TCCTCAGGGC 
CAAGCGCACC CACGGCGCAT GCCAAATGGC TACCGTTCGT CTTCGCCCTC TCCACACCAC 
CACGCGCCCG CTCACCCTGG AACTCATAAA CCTCCTCATC CCAGAGGGCC CAGAAAGGGC 
CTCCATGAAC CCTACAGTGA GACTGACGAG GATGACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_017354796.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017210285.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X7, mRNA.

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CloneID	ODa58252
Clone ID Related Accession (Same CDS sequence)	XM_017354796.1
Accession Version	XM_017354796.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGTGGCAGAA TCAGTTACAG GGATATGTAT GAGATGCTCA GGCACATGTC TCCTCCGTTA 
GGCCTGGGGA AGAAGTGCCC TGCACGGGTT GCCTACAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGAGGT GTAGATAAGC ACCAGATGGA TGCTGAATTA 
AGGAAGGAGA TGATGGCGAT ATGGCCTAAT CTCTCTCAGA AGAACCTGGA CCTGCTGGTG 
ACTCCTCACA AGTCAGCGAC AGACCTGACA GTAGGAAAGA TCTACGCTGC CATGATGATC 
ATGGAGTATT ATCGGCAAAG TAAAGCCAAA CGGACACAAG CTCTCCATGA TGAGCAGAAT 
CGAACGCCAT TAATGTTTCA GCGTCTGGAG CCGCCATCTC CTAGCCAGGA TGTGGGACCA 
GGACTCACTG GCCTTCCTGA CACACAAATG CACCCTGCAA ATCATCTGCC TGTTGATGAA 
AGGATCCCAG AGAGCCAATC ATGGGTGACA GCGCGAGCAC AGGAGATTTC TCAAAAAGCA 
GGCAACTGGA GTCCAGAGGG CCAACATCCA GATGACACCA CAGACAACAG AAGGCAGTCT 
CAGACAGTAG AGATGAGAGA GATGGGGCGG GACGGGTACT CGGACACAGA GCAATATCTG 
CCGATGGAAG GCCATGGCAG GGCTGCTTCC ATGCCTCGAC TCCCAGCAGA CAACCAACAG 
CCTATCACAG ATAACAGTCC TATGAAGCGC TCGGCTTCAT CTTTGGGACA CGGGCGTGCA 
GGACGCTCCA TGCGAGGTGA GGATTATGCC ATGGAGAGGG TTATCCCAGA AGAGGGTCAC 
AGGCATGGGC ATCGCCACCG AGACCGTAGT CACCGGGCGT CAGAACGCTC CCTGAGCCGA 
TACACTGATG CTGACACAGG TCTTGGAACT GACCTCAGCA CAACCACACA ATCAGGAGAC 
CTGCCATCCA AGGAGAGAGA CCGGGGCCGA GCCAAAGACC GCAAGCACCA TCATCATCAT 
CATCACCACC ACGGCTCTTT AGATAAGGAG CACTACGGTC ATGAGAGAGA CAGAGGAGAG 
TACGGCCACA GACAGTCCAG AGAGAGAGAC CGCCGCTGGT CCCGCTCACC CAGTGAGGGC 
AGAGAGTGTC TGACTCACAG ACAGGGCAGT AGTTCAGTAA GCGGCAGTCC AGTCCCGTCC 
ACCTCCGGGA CCAGCACCCC CCGCCGTGGC CGCCGTCAGC TCCCCCAAAC CCCTGCTACA 
CCCCGGCCAC ATGTCACCTA CTCACCTGTG GTCCGTAAGC CCATCAGCAG TACCCCCCCT 
CCTGGCCAGC AGAGCCGACT CCCTACCCCG ACCTCCCGGC GCTTCAGTCC GACTGGCCCC 
GAGCCCCCGC TTCCCCCTCA TCACCACCCG TCACCACCCC ACCACGGCTC TCCCCGCTCC 
GGCCGACATG CACACTGGGG CCCCGAGCCT GCAGAGAGCG TGGAGGGTGA CGGCTTCTAT 
GACGACCAAG ACTATGAGTT CAATCACCAC GAGCCTCCAT CCTACGAGCA AAGCCCTGTG 
CAGGGCAACC CTCACCCCCA TTCCCCTAGG ACTTCCCGCC ACAACACCCC TCCTCAGGGC 
CAAGCGCACC CACGGCGCAT GCCAAATGGC TACCGTTCGT CTTCGCCCTC TCCACACCAC 
CACGCGCCCG CTCACCCTGG AACTCATAAA CCTCCTCATC CCAGAGGGCC CAGAAAGGGC 
CTCCATGAAC CCTACAGTGA GACTGACGAG GATGACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210285.1
CDS	1467..8531
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGGSGGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGMNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRISYRDMYEMLRHMSPPLGLGKKCPARVAYKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQNRTPLMFQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQTVEMREMGRDGYSDTEQYLPMEGHGRAASMPRLPADNQQPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_017354796.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354796.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGTGGCAGAA TCAGTTACAG GGATATGTAT GAGATGCTCA GGCACATGTC TCCTCCGTTA 
GGCCTGGGGA AGAAGTGCCC TGCACGGGTT GCCTACAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGAGGT GTAGATAAGC ACCAGATGGA TGCTGAATTA 
AGGAAGGAGA TGATGGCGAT ATGGCCTAAT CTCTCTCAGA AGAACCTGGA CCTGCTGGTG 
ACTCCTCACA AGTCAGCGAC AGACCTGACA GTAGGAAAGA TCTACGCTGC CATGATGATC 
ATGGAGTATT ATCGGCAAAG TAAAGCCAAA CGGACACAAG CTCTCCATGA TGAGCAGAAT 
CGAACGCCAT TAATGTTTCA GCGTCTGGAG CCGCCATCTC CTAGCCAGGA TGTGGGACCA 
GGACTCACTG GCCTTCCTGA CACACAAATG CACCCTGCAA ATCATCTGCC TGTTGATGAA 
AGGATCCCAG AGAGCCAATC ATGGGTGACA GCGCGAGCAC AGGAGATTTC TCAAAAAGCA 
GGCAACTGGA GTCCAGAGGG CCAACATCCA GATGACACCA CAGACAACAG AAGGCAGTCT 
CAGACAGTAG AGATGAGAGA GATGGGGCGG GACGGGTACT CGGACACAGA GCAATATCTG 
CCGATGGAAG GCCATGGCAG GGCTGCTTCC ATGCCTCGAC TCCCAGCAGA CAACCAACAG 
CCTATCACAG ATAACAGTCC TATGAAGCGC TCGGCTTCAT CTTTGGGACA CGGGCGTGCA 
GGACGCTCCA TGCGAGGTGA GGATTATGCC ATGGAGAGGG TTATCCCAGA AGAGGGTCAC 
AGGCATGGGC ATCGCCACCG AGACCGTAGT CACCGGGCGT CAGAACGCTC CCTGAGCCGA 
TACACTGATG CTGACACAGG TCTTGGAACT GACCTCAGCA CAACCACACA ATCAGGAGAC 
CTGCCATCCA AGGAGAGAGA CCGGGGCCGA GCCAAAGACC GCAAGCACCA TCATCATCAT 
CATCACCACC ACGGCTCTTT AGATAAGGAG CACTACGGTC ATGAGAGAGA CAGAGGAGAG 
TACGGCCACA GACAGTCCAG AGAGAGAGAC CGCCGCTGGT CCCGCTCACC CAGTGAGGGC 
AGAGAGTGTC TGACTCACAG ACAGGGCAGT AGTTCAGTAA GCGGCAGTCC AGTCCCGTCC 
ACCTCCGGGA CCAGCACCCC CCGCCGTGGC CGCCGTCAGC TCCCCCAAAC CCCTGCTACA 
CCCCGGCCAC ATGTCACCTA CTCACCTGTG GTCCGTAAGC CCATCAGCAG TACCCCCCCT 
CCTGGCCAGC AGAGCCGACT CCCTACCCCG ACCTCCCGGC GCTTCAGTCC GACTGGCCCC 
GAGCCCCCGC TTCCCCCTCA TCACCACCCG TCACCACCCC ACCACGGCTC TCCCCGCTCC 
GGCCGACATG CACACTGGGG CCCCGAGCCT GCAGAGAGCG TGGAGGGTGA CGGCTTCTAT 
GACGACCAAG ACTATGAGTT CAATCACCAC GAGCCTCCAT CCTACGAGCA AAGCCCTGTG 
CAGGGCAACC CTCACCCCCA TTCCCCTAGG ACTTCCCGCC ACAACACCCC TCCTCAGGGC 
CAAGCGCACC CACGGCGCAT GCCAAATGGC TACCGTTCGT CTTCGCCCTC TCCACACCAC 
CACGCGCCCG CTCACCCTGG AACTCATAAA CCTCCTCATC CCAGAGGGCC CAGAAAGGGC 
CTCCATGAAC CCTACAGTGA GACTGACGAG GATGACTGGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_017354797.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017210286.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X8, mRNA.

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CloneID	ODa58253
Clone ID Related Accession (Same CDS sequence)	XM_017354797.1
Accession Version	XM_017354797.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACAGGAAGC AGCAAAGGGA AAGACATCAG TACTATCAAG 
TCTCTACGTG TGCTTCGAGT ACTGCGGCCG CTGAAAACCA TCAAACGTCT GCCAAAACTG 
AAGGCTGTCT TTGACTGTGT GGTGAACTCT CTGAAGAATG TGTTGAACAT CCTCATCGTC 
TACATGCTCT TCATGTTCAT CTTCGCTGTG GTGGCTGTGC AGCTCTTTAA GGGACGTTTC 
TTTTACTGCA CTGATGAATC CAAAGAGTTT GAGCGCGACT GCAGGGGCGA GTATCTGGTC 
TATGAAAGAG ATAACGAAGT GAGGTCACAG AAGCGGGAAT GGAAGAAATA CGATTTCCAC 
TACGACAACG TGCTCTGGGC CCTTCTCACC CTCTTCACCG TTTCTACGGG AGAGGGGTGG 
CCGCAAGTGC TAAAACATTC AGTAGATGCG ACCTACGAGA ATCAGGGCCC GAGCCCAGGT 
TACCGGATGG AAATGTCCAT CTTTTACGTG GTGTACTTCG TGGTCTTCCC CTTCTTCTTC 
GTCAACATCT TCGTGGCTCT TATCATCATT ACTTTCCAGG AGCAAGGAGA CAAAATGATG 
GAGGACTATA GCCTGGAAAA GAATGAGAGA GCCTGCATAG ACTTTGCCAT CAACGCTAAG 
CCGCTCACAC GGCACATGCC CCAGAACAAG CAGACCTTTC AGTACCGCAT GTGGGAGTTT 
GTGGTGTCTC CTCCATTTGA ATACACCATC ATGGCCTTGA TCGCGCTCAA CACTATTGTC 
CTTATGATGA AGTATGATGG AGCATCGCTT ACTTATGAAG ATGTGCTAAA ATACCTGAAC 
ATAGTGTTCA CCTCTCTATT CTCCATGGAG TGCATATTGA AGATCATAGC CTTCGGAGCA 
TTGAACTACT TCAAAGATGC CTGGAACATC TTCGACTGTG TGACTGTACT TGGCAGCATC 
ACTGATATTT TAGTCACAGA GCTGGGGATG AACAACTTCA TAAATCTGAG TTTTCTAAGG 
CTCTTCAGAG CTGCTCGTCT CATTAAGTTG CTCAGGCAGG GTGAAACCAT ACGCATCCTG 
CTCTGGACTT TTGTCCAGTC TTTCAAGGCT CTGCCGTATG TGTGCCTACT CATCGCCATG 
CTGTTCTTCA TCTACGCCAT CATTGGCATG CAGCTGTTTG GGAACATTAA GATCGAGGAG 
AACAGTGACA GTGCCATTAC CCAGCACAAC AACTTCAGGA CTTTTTTCCA GGCCCTAATG 
CTGCTCTTTA GGAGTGCTAC AGGTGAGGCA TGGCATGACA TCATGCTGTC ATGTCTGGGA 
AAGAAGCCGT GTGATATTCT CTCTGATAAC CCGAAGCCAG AATGCGGGAG CGAGTTTGCC 
TACCTCTACT TTGTCTCCTT CATCTTCCTC TGTTCATTTC TGATGCTGAA TCTGTTCGTG 
GCTGTGATCA TGGATAATTT TGAATACCTG ACGCGTGACT CCTCTATCCT GGGACCCCAT 
CACCTGGACG AGTATGTGAG GATCTGGGCA GAATATGATC CTGCTGCTTG CGGGCGCATT 
CATTATAAGG ATATGTACAG TTTATTGCGA GTTATCGACC CCCCTCTGGG CTTAGGCAAG 
AAATGTCCTC ATAGGGTGGC CTGCAAGAGA CTGTTGCGGA TGGATCTGCC GGTTGCCGAT 
GACAACACAG TGCACTTTAA TTCTACCCTT ATGGCCTTAA TCCGAACCGC TCTGGACATC 
AAGATCGCCA AGGGTGGGGA AGGAGGTGTA GATAAGCACC AGATGGATGC TGAATTAAGG 
AAGGAGATGA TGGCGATATG GCCTAATCTC TCTCAGAAGA ACCTGGACCT GCTGGTGACT 
CCTCACAAGT CAGCGACAGA CCTGACAGTA GGAAAGATCT ACGCTGCCAT GATGATCATG 
GAGTATTATC GGCAAAGTAA AGCCAAACGG ACACAAGCTC TCCATGATGA GCAGAATCGA 
ACGCCATTAA TGTTTCAGCG TCTGGAGCCG CCATCTCCTA GCCAGGATGT GGGACCAGGA 
CTCACTGGCC TTCCTGACAC ACAAATGCAC CCTGCAAATC ATCTGCCTGT TGATGAAAGG 
ATCCCAGAGA GCCAATCATG GGTGACAGCG CGAGCACAGG AGATTTCTCA AAAAGCAGGC 
AACTGGAGTC CAGAGGGCCA ACATCCAGAT GACACCACAG ACAACAGAAG GCAGTCTCAG 
ACAGTAGAGA TGAGAGAGAT GGGGCGGGAC GGGTACTCGG ACACAGAGCA ATATCTGCCG 
ATGGAAGGCC ATGGCAGGGC TGCTTCCATG CCTCGACTCC CAGCAGACAA CCAACAGCCT 
ATCACAGATA ACAGTCCTAT GAAGCGCTCG GCTTCATCTT TGGGACACGG GCGTGCAGGA 
CGCTCCATGC GAGGTGAGGA TTATGCCATG GAGAGGGTTA TCCCAGAAGA GGGTCACAGG 
CATGGGCATC GCCACCGAGA CCGTAGTCAC CGGGCGTCAG AACGCTCCCT GAGCCGATAC 
ACTGATGCTG ACACAGGTCT TGGAACTGAC CTCAGCACAA CCACACAATC AGGAGACCTG 
CCATCCAAGG AGAGAGACCG GGGCCGAGCC AAAGACCGCA AGCACCATCA TCATCATCAT 
CACCACCACG GCTCTTTAGA TAAGGAGCAC TACGGTCATG AGAGAGACAG AGGAGAGTAC 
GGCCACAGAC AGTCCAGAGA GAGAGACCGC CGCTGGTCCC GCTCACCCAG TGAGGGCAGA 
GAGTGTCTGA CTCACAGACA GGGCAGTAGT TCAGTAAGCG GCAGTCCAGT CCCGTCCACC 
TCCGGGACCA GCACCCCCCG CCGTGGCCGC CGTCAGCTCC CCCAAACCCC TGCTACACCC 
CGGCCACATG TCACCTACTC ACCTGTGGTC CGTAAGCCCA TCAGCAGTAC CCCCCCTCCT 
GGCCAGCAGA GCCGACTCCC TACCCCGACC TCCCGGCGCT TCAGTCCGAC TGGCCCCGAG 
CCCCCGCTTC CCCCTCATCA CCACCCGTCA CCACCCCACC ACGGCTCTCC CCGCTCCGGC 
CGACATGCAC ACTGGGGCCC CGAGCCTGCA GAGAGCGTGG AGGGTGACGG CTTCTATGAC 
GACCAAGACT ATGAGTTCAA TCACCACGAG CCTCCATCCT ACGAGCAAAG CCCTGTGCAG 
GGCAACCCTC ACCCCCATTC CCCTAGGACT TCCCGCCACA ACACCCCTCC TCAGGGCCAA 
GCGCACCCAC GGCGCATGCC AAATGGCTAC CGTTCGTCTT CGCCCTCTCC ACACCACCAC 
GCGCCCGCTC ACCCTGGAAC TCATAAACCT CCTCATCCCA GAGGGCCCAG AAAGGGCCTC 
CATGAACCCT ACAGTGAGAC TGACGAGGAT GACTGGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210286.1
CDS	1465..8526
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGMNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGEGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQNRTPLMFQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQTVEMREMGRDGYSDTEQYLPMEGHGRAASMPRLPADNQQPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_017354797.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354797.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACAGGAAGC AGCAAAGGGA AAGACATCAG TACTATCAAG 
TCTCTACGTG TGCTTCGAGT ACTGCGGCCG CTGAAAACCA TCAAACGTCT GCCAAAACTG 
AAGGCTGTCT TTGACTGTGT GGTGAACTCT CTGAAGAATG TGTTGAACAT CCTCATCGTC 
TACATGCTCT TCATGTTCAT CTTCGCTGTG GTGGCTGTGC AGCTCTTTAA GGGACGTTTC 
TTTTACTGCA CTGATGAATC CAAAGAGTTT GAGCGCGACT GCAGGGGCGA GTATCTGGTC 
TATGAAAGAG ATAACGAAGT GAGGTCACAG AAGCGGGAAT GGAAGAAATA CGATTTCCAC 
TACGACAACG TGCTCTGGGC CCTTCTCACC CTCTTCACCG TTTCTACGGG AGAGGGGTGG 
CCGCAAGTGC TAAAACATTC AGTAGATGCG ACCTACGAGA ATCAGGGCCC GAGCCCAGGT 
TACCGGATGG AAATGTCCAT CTTTTACGTG GTGTACTTCG TGGTCTTCCC CTTCTTCTTC 
GTCAACATCT TCGTGGCTCT TATCATCATT ACTTTCCAGG AGCAAGGAGA CAAAATGATG 
GAGGACTATA GCCTGGAAAA GAATGAGAGA GCCTGCATAG ACTTTGCCAT CAACGCTAAG 
CCGCTCACAC GGCACATGCC CCAGAACAAG CAGACCTTTC AGTACCGCAT GTGGGAGTTT 
GTGGTGTCTC CTCCATTTGA ATACACCATC ATGGCCTTGA TCGCGCTCAA CACTATTGTC 
CTTATGATGA AGTATGATGG AGCATCGCTT ACTTATGAAG ATGTGCTAAA ATACCTGAAC 
ATAGTGTTCA CCTCTCTATT CTCCATGGAG TGCATATTGA AGATCATAGC CTTCGGAGCA 
TTGAACTACT TCAAAGATGC CTGGAACATC TTCGACTGTG TGACTGTACT TGGCAGCATC 
ACTGATATTT TAGTCACAGA GCTGGGGATG AACAACTTCA TAAATCTGAG TTTTCTAAGG 
CTCTTCAGAG CTGCTCGTCT CATTAAGTTG CTCAGGCAGG GTGAAACCAT ACGCATCCTG 
CTCTGGACTT TTGTCCAGTC TTTCAAGGCT CTGCCGTATG TGTGCCTACT CATCGCCATG 
CTGTTCTTCA TCTACGCCAT CATTGGCATG CAGCTGTTTG GGAACATTAA GATCGAGGAG 
AACAGTGACA GTGCCATTAC CCAGCACAAC AACTTCAGGA CTTTTTTCCA GGCCCTAATG 
CTGCTCTTTA GGAGTGCTAC AGGTGAGGCA TGGCATGACA TCATGCTGTC ATGTCTGGGA 
AAGAAGCCGT GTGATATTCT CTCTGATAAC CCGAAGCCAG AATGCGGGAG CGAGTTTGCC 
TACCTCTACT TTGTCTCCTT CATCTTCCTC TGTTCATTTC TGATGCTGAA TCTGTTCGTG 
GCTGTGATCA TGGATAATTT TGAATACCTG ACGCGTGACT CCTCTATCCT GGGACCCCAT 
CACCTGGACG AGTATGTGAG GATCTGGGCA GAATATGATC CTGCTGCTTG CGGGCGCATT 
CATTATAAGG ATATGTACAG TTTATTGCGA GTTATCGACC CCCCTCTGGG CTTAGGCAAG 
AAATGTCCTC ATAGGGTGGC CTGCAAGAGA CTGTTGCGGA TGGATCTGCC GGTTGCCGAT 
GACAACACAG TGCACTTTAA TTCTACCCTT ATGGCCTTAA TCCGAACCGC TCTGGACATC 
AAGATCGCCA AGGGTGGGGA AGGAGGTGTA GATAAGCACC AGATGGATGC TGAATTAAGG 
AAGGAGATGA TGGCGATATG GCCTAATCTC TCTCAGAAGA ACCTGGACCT GCTGGTGACT 
CCTCACAAGT CAGCGACAGA CCTGACAGTA GGAAAGATCT ACGCTGCCAT GATGATCATG 
GAGTATTATC GGCAAAGTAA AGCCAAACGG ACACAAGCTC TCCATGATGA GCAGAATCGA 
ACGCCATTAA TGTTTCAGCG TCTGGAGCCG CCATCTCCTA GCCAGGATGT GGGACCAGGA 
CTCACTGGCC TTCCTGACAC ACAAATGCAC CCTGCAAATC ATCTGCCTGT TGATGAAAGG 
ATCCCAGAGA GCCAATCATG GGTGACAGCG CGAGCACAGG AGATTTCTCA AAAAGCAGGC 
AACTGGAGTC CAGAGGGCCA ACATCCAGAT GACACCACAG ACAACAGAAG GCAGTCTCAG 
ACAGTAGAGA TGAGAGAGAT GGGGCGGGAC GGGTACTCGG ACACAGAGCA ATATCTGCCG 
ATGGAAGGCC ATGGCAGGGC TGCTTCCATG CCTCGACTCC CAGCAGACAA CCAACAGCCT 
ATCACAGATA ACAGTCCTAT GAAGCGCTCG GCTTCATCTT TGGGACACGG GCGTGCAGGA 
CGCTCCATGC GAGGTGAGGA TTATGCCATG GAGAGGGTTA TCCCAGAAGA GGGTCACAGG 
CATGGGCATC GCCACCGAGA CCGTAGTCAC CGGGCGTCAG AACGCTCCCT GAGCCGATAC 
ACTGATGCTG ACACAGGTCT TGGAACTGAC CTCAGCACAA CCACACAATC AGGAGACCTG 
CCATCCAAGG AGAGAGACCG GGGCCGAGCC AAAGACCGCA AGCACCATCA TCATCATCAT 
CACCACCACG GCTCTTTAGA TAAGGAGCAC TACGGTCATG AGAGAGACAG AGGAGAGTAC 
GGCCACAGAC AGTCCAGAGA GAGAGACCGC CGCTGGTCCC GCTCACCCAG TGAGGGCAGA 
GAGTGTCTGA CTCACAGACA GGGCAGTAGT TCAGTAAGCG GCAGTCCAGT CCCGTCCACC 
TCCGGGACCA GCACCCCCCG CCGTGGCCGC CGTCAGCTCC CCCAAACCCC TGCTACACCC 
CGGCCACATG TCACCTACTC ACCTGTGGTC CGTAAGCCCA TCAGCAGTAC CCCCCCTCCT 
GGCCAGCAGA GCCGACTCCC TACCCCGACC TCCCGGCGCT TCAGTCCGAC TGGCCCCGAG 
CCCCCGCTTC CCCCTCATCA CCACCCGTCA CCACCCCACC ACGGCTCTCC CCGCTCCGGC 
CGACATGCAC ACTGGGGCCC CGAGCCTGCA GAGAGCGTGG AGGGTGACGG CTTCTATGAC 
GACCAAGACT ATGAGTTCAA TCACCACGAG CCTCCATCCT ACGAGCAAAG CCCTGTGCAG 
GGCAACCCTC ACCCCCATTC CCCTAGGACT TCCCGCCACA ACACCCCTCC TCAGGGCCAA 
GCGCACCCAC GGCGCATGCC AAATGGCTAC CGTTCGTCTT CGCCCTCTCC ACACCACCAC 
GCGCCCGCTC ACCCTGGAAC TCATAAACCT CCTCATCCCA GAGGGCCCAG AAAGGGCCTC 
CATGAACCCT ACAGTGAGAC TGACGAGGAT GACTGGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_017354798.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017210287.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X9, mRNA.

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CloneID	ODa58254
Clone ID Related Accession (Same CDS sequence)	XM_017354798.1
Accession Version	XM_017354798.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGTGGCAGAA TCAGTTACAG GGATATGTAT GAGATGCTCA GGCACATGTC TCCTCCGTTA 
GGCCTGGGGA AGAAGTGCCC TGCACGGGTT GCCTACAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGAGGT GTAGATAAGC ACCAGATGGA TGCTGAATTA 
AGGAAGGAGA TGATGGCGAT ATGGCCTAAT CTCTCTCAGA AGAACCTGGA CCTGCTGGTG 
ACTCCTCACA AGTCGACAGA CCTGACAGTA GGAAAGATCT ACGCTGCCAT GATGATCATG 
GAGTATTATC GGCAAAGTAA AGCCAAACGG ACACAAGCTC TCCATGATGA GCAGAATCGA 
ACGCCATTAA TGTTTCAGCG TCTGGAGCCG CCATCTCCTA GCCAGGATGT GGGACCAGGA 
CTCACTGGCC TTCCTGACAC ACAAATGCAC CCTGCAAATC ATCTGCCTGT TGATGAAAGG 
ATCCCAGAGA GCCAATCATG GGTGACAGCG CGAGCACAGG AGATTTCTCA AAAAGCAGGC 
AACTGGAGTC CAGAGGGCCA ACATCCAGAT GACACCACAG ACAACAGAAG GCAGTCTCAG 
ACAGTAGAGA TGAGAGAGAT GGGGCGGGAC GGGTACTCGG ACACAGAGCA ATATCTGCCG 
ATGGAAGGCC ATGGCAGGGC TGCTTCCATG CCTCGACTCC CAGCAGACAA CCAACAGCCT 
ATCACAGATA ACAGTCCTAT GAAGCGCTCG GCTTCATCTT TGGGACACGG GCGTGCAGGA 
CGCTCCATGC GAGGTGAGGA TTATGCCATG GAGAGGGTTA TCCCAGAAGA GGGTCACAGG 
CATGGGCATC GCCACCGAGA CCGTAGTCAC CGGGCGTCAG AACGCTCCCT GAGCCGATAC 
ACTGATGCTG ACACAGGTCT TGGAACTGAC CTCAGCACAA CCACACAATC AGGAGACCTG 
CCATCCAAGG AGAGAGACCG GGGCCGAGCC AAAGACCGCA AGCACCATCA TCATCATCAT 
CACCACCACG GCTCTTTAGA TAAGGAGCAC TACGGTCATG AGAGAGACAG AGGAGAGTAC 
GGCCACAGAC AGTCCAGAGA GAGAGACCGC CGCTGGTCCC GCTCACCCAG TGAGGGCAGA 
GAGTGTCTGA CTCACAGACA GGGCAGTAGT TCAGTAAGCG GCAGTCCAGT CCCGTCCACC 
TCCGGGACCA GCACCCCCCG CCGTGGCCGC CGTCAGCTCC CCCAAACCCC TGCTACACCC 
CGGCCACATG TCACCTACTC ACCTGTGGTC CGTAAGCCCA TCAGCAGTAC CCCCCCTCCT 
GGCCAGCAGA GCCGACTCCC TACCCCGACC TCCCGGCGCT TCAGTCCGAC TGGCCCCGAG 
CCCCCGCTTC CCCCTCATCA CCACCCGTCA CCACCCCACC ACGGCTCTCC CCGCTCCGGC 
CGACATGCAC ACTGGGGCCC CGAGCCTGCA GAGAGCGTGG AGGGTGACGG CTTCTATGAC 
GACCAAGACT ATGAGTTCAA TCACCACGAG CCTCCATCCT ACGAGCAAAG CCCTGTGCAG 
GGCAACCCTC ACCCCCATTC CCCTAGGACT TCCCGCCACA ACACCCCTCC TCAGGGCCAA 
GCGCACCCAC GGCGCATGCC AAATGGCTAC CGTTCGTCTT CGCCCTCTCC ACACCACCAC 
GCGCCCGCTC ACCCTGGAAC TCATAAACCT CCTCATCCCA GAGGGCCCAG AAAGGGCCTC 
CATGAACCCT ACAGTGAGAC TGACGAGGAT GACTGGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210287.1
CDS	1468..8529
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGGSGGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGMNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRISYRDMYEMLRHMSPPLGLGKKCPARVAYKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQNRTPLMFQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQTVEMREMGRDGYSDTEQYLPMEGHGRAASMPRLPADNQQPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_017354798.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354798.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGTGGCAGAA TCAGTTACAG GGATATGTAT GAGATGCTCA GGCACATGTC TCCTCCGTTA 
GGCCTGGGGA AGAAGTGCCC TGCACGGGTT GCCTACAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGAGGT GTAGATAAGC ACCAGATGGA TGCTGAATTA 
AGGAAGGAGA TGATGGCGAT ATGGCCTAAT CTCTCTCAGA AGAACCTGGA CCTGCTGGTG 
ACTCCTCACA AGTCGACAGA CCTGACAGTA GGAAAGATCT ACGCTGCCAT GATGATCATG 
GAGTATTATC GGCAAAGTAA AGCCAAACGG ACACAAGCTC TCCATGATGA GCAGAATCGA 
ACGCCATTAA TGTTTCAGCG TCTGGAGCCG CCATCTCCTA GCCAGGATGT GGGACCAGGA 
CTCACTGGCC TTCCTGACAC ACAAATGCAC CCTGCAAATC ATCTGCCTGT TGATGAAAGG 
ATCCCAGAGA GCCAATCATG GGTGACAGCG CGAGCACAGG AGATTTCTCA AAAAGCAGGC 
AACTGGAGTC CAGAGGGCCA ACATCCAGAT GACACCACAG ACAACAGAAG GCAGTCTCAG 
ACAGTAGAGA TGAGAGAGAT GGGGCGGGAC GGGTACTCGG ACACAGAGCA ATATCTGCCG 
ATGGAAGGCC ATGGCAGGGC TGCTTCCATG CCTCGACTCC CAGCAGACAA CCAACAGCCT 
ATCACAGATA ACAGTCCTAT GAAGCGCTCG GCTTCATCTT TGGGACACGG GCGTGCAGGA 
CGCTCCATGC GAGGTGAGGA TTATGCCATG GAGAGGGTTA TCCCAGAAGA GGGTCACAGG 
CATGGGCATC GCCACCGAGA CCGTAGTCAC CGGGCGTCAG AACGCTCCCT GAGCCGATAC 
ACTGATGCTG ACACAGGTCT TGGAACTGAC CTCAGCACAA CCACACAATC AGGAGACCTG 
CCATCCAAGG AGAGAGACCG GGGCCGAGCC AAAGACCGCA AGCACCATCA TCATCATCAT 
CACCACCACG GCTCTTTAGA TAAGGAGCAC TACGGTCATG AGAGAGACAG AGGAGAGTAC 
GGCCACAGAC AGTCCAGAGA GAGAGACCGC CGCTGGTCCC GCTCACCCAG TGAGGGCAGA 
GAGTGTCTGA CTCACAGACA GGGCAGTAGT TCAGTAAGCG GCAGTCCAGT CCCGTCCACC 
TCCGGGACCA GCACCCCCCG CCGTGGCCGC CGTCAGCTCC CCCAAACCCC TGCTACACCC 
CGGCCACATG TCACCTACTC ACCTGTGGTC CGTAAGCCCA TCAGCAGTAC CCCCCCTCCT 
GGCCAGCAGA GCCGACTCCC TACCCCGACC TCCCGGCGCT TCAGTCCGAC TGGCCCCGAG 
CCCCCGCTTC CCCCTCATCA CCACCCGTCA CCACCCCACC ACGGCTCTCC CCGCTCCGGC 
CGACATGCAC ACTGGGGCCC CGAGCCTGCA GAGAGCGTGG AGGGTGACGG CTTCTATGAC 
GACCAAGACT ATGAGTTCAA TCACCACGAG CCTCCATCCT ACGAGCAAAG CCCTGTGCAG 
GGCAACCCTC ACCCCCATTC CCCTAGGACT TCCCGCCACA ACACCCCTCC TCAGGGCCAA 
GCGCACCCAC GGCGCATGCC AAATGGCTAC CGTTCGTCTT CGCCCTCTCC ACACCACCAC 
GCGCCCGCTC ACCCTGGAAC TCATAAACCT CCTCATCCCA GAGGGCCCAG AAAGGGCCTC 
CATGAACCCT ACAGTGAGAC TGACGAGGAT GACTGGTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_017354799.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017210288.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X11, mRNA.

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CloneID	ODa58255
Clone ID Related Accession (Same CDS sequence)	XM_017354799.1
Accession Version	XM_017354799.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG 
GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT 
GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC 
CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC 
ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT 
GAGCAGCGTC TGGAGCCGCC ATCTCCTAGC CAGGATGTGG GACCAGGACT CACTGGCCTT 
CCTGACACAC AAATGCACCC TGCAAATCAT CTGCCTGTTG ATGAAAGGAT CCCAGAGAGC 
CAATCATGGG TGACAGCGCG AGCACAGGAG ATTTCTCAAA AAGCAGGCAA CTGGAGTCCA 
GAGGGCCAAC ATCCAGATGA CACCACAGAC AACAGAAGGC AGTCTCAGAC AGTAGAGATG 
AGAGAGATGG GGCGGGACGG GTACTCGGAC ACAGAGCAAT ATCTGCCGAT GGAAGGCCAT 
GGCAGGGCTG CTTCCATGCC TCGACTCCCA GCAGACAACC AACAGCCTAT CACAGATAAC 
AGTCCTATGA AGCGCTCGGC TTCATCTTTG GGACACGGGC GTGCAGGACG CTCCATGCGA 
GGTGAGGATT ATGCCATGGA GAGGGTTATC CCAGAAGAGG GTCACAGGCA TGGGCATCGC 
CACCGAGACC GTAGTCACCG GGCGTCAGAA CGCTCCCTGA GCCGATACAC TGATGCTGAC 
ACAGGTCTTG GAACTGACCT CAGCACAACC ACACAATCAG GAGACCTGCC ATCCAAGGAG 
AGAGACCGGG GCCGAGCCAA AGACCGCAAG CACCATCATC ATCATCATCA CCACCACGGC 
TCTTTAGATA AGGAGCACTA CGGTCATGAG AGAGACAGAG GAGAGTACGG CCACAGACAG 
TCCAGAGAGA GAGACCGCCG CTGGTCCCGC TCACCCAGTG AGGGCAGAGA GTGTCTGACT 
CACAGACAGG GCAGTAGTTC AGTAAGCGGC AGTCCAGTCC CGTCCACCTC CGGGACCAGC 
ACCCCCCGCC GTGGCCGCCG TCAGCTCCCC CAAACCCCTG CTACACCCCG GCCACATGTC 
ACCTACTCAC CTGTGGTCCG TAAGCCCATC AGCAGTACCC CCCCTCCTGG CCAGCAGAGC 
CGACTCCCTA CCCCGACCTC CCGGCGCTTC AGTCCGACTG GCCCCGAGCC CCCGCTTCCC 
CCTCATCACC ACCCGTCACC ACCCCACCAC GGCTCTCCCC GCTCCGGCCG ACATGCACAC 
TGGGGCCCCG AGCCTGCAGA GAGCGTGGAG GGTGACGGCT TCTATGACGA CCAAGACTAT 
GAGTTCAATC ACCACGAGCC TCCATCCTAC GAGCAAAGCC CTGTGCAGGG CAACCCTCAC 
CCCCATTCCC CTAGGACTTC CCGCCACAAC ACCCCTCCTC AGGGCCAAGC GCACCCACGG 
CGCATGCCAA ATGGCTACCG TTCGTCTTCG CCCTCTCCAC ACCACCACGC GCCCGCTCAC 
CCTGGAACTC ATAAACCTCC TCATCCCAGA GGGCCCAGAA AGGGCCTCCA TGAACCCTAC 
AGTGAGACTG ACGAGGATGA CTGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210288.1
CDS	1468..8517
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGGSGGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGMNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGEGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQTVEMREMGRDGYSDTEQYLPMEGHGRAASMPRLPADNQQPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_017354799.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354799.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG 
GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT 
GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC 
CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC 
ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT 
GAGCAGCGTC TGGAGCCGCC ATCTCCTAGC CAGGATGTGG GACCAGGACT CACTGGCCTT 
CCTGACACAC AAATGCACCC TGCAAATCAT CTGCCTGTTG ATGAAAGGAT CCCAGAGAGC 
CAATCATGGG TGACAGCGCG AGCACAGGAG ATTTCTCAAA AAGCAGGCAA CTGGAGTCCA 
GAGGGCCAAC ATCCAGATGA CACCACAGAC AACAGAAGGC AGTCTCAGAC AGTAGAGATG 
AGAGAGATGG GGCGGGACGG GTACTCGGAC ACAGAGCAAT ATCTGCCGAT GGAAGGCCAT 
GGCAGGGCTG CTTCCATGCC TCGACTCCCA GCAGACAACC AACAGCCTAT CACAGATAAC 
AGTCCTATGA AGCGCTCGGC TTCATCTTTG GGACACGGGC GTGCAGGACG CTCCATGCGA 
GGTGAGGATT ATGCCATGGA GAGGGTTATC CCAGAAGAGG GTCACAGGCA TGGGCATCGC 
CACCGAGACC GTAGTCACCG GGCGTCAGAA CGCTCCCTGA GCCGATACAC TGATGCTGAC 
ACAGGTCTTG GAACTGACCT CAGCACAACC ACACAATCAG GAGACCTGCC ATCCAAGGAG 
AGAGACCGGG GCCGAGCCAA AGACCGCAAG CACCATCATC ATCATCATCA CCACCACGGC 
TCTTTAGATA AGGAGCACTA CGGTCATGAG AGAGACAGAG GAGAGTACGG CCACAGACAG 
TCCAGAGAGA GAGACCGCCG CTGGTCCCGC TCACCCAGTG AGGGCAGAGA GTGTCTGACT 
CACAGACAGG GCAGTAGTTC AGTAAGCGGC AGTCCAGTCC CGTCCACCTC CGGGACCAGC 
ACCCCCCGCC GTGGCCGCCG TCAGCTCCCC CAAACCCCTG CTACACCCCG GCCACATGTC 
ACCTACTCAC CTGTGGTCCG TAAGCCCATC AGCAGTACCC CCCCTCCTGG CCAGCAGAGC 
CGACTCCCTA CCCCGACCTC CCGGCGCTTC AGTCCGACTG GCCCCGAGCC CCCGCTTCCC 
CCTCATCACC ACCCGTCACC ACCCCACCAC GGCTCTCCCC GCTCCGGCCG ACATGCACAC 
TGGGGCCCCG AGCCTGCAGA GAGCGTGGAG GGTGACGGCT TCTATGACGA CCAAGACTAT 
GAGTTCAATC ACCACGAGCC TCCATCCTAC GAGCAAAGCC CTGTGCAGGG CAACCCTCAC 
CCCCATTCCC CTAGGACTTC CCGCCACAAC ACCCCTCCTC AGGGCCAAGC GCACCCACGG 
CGCATGCCAA ATGGCTACCG TTCGTCTTCG CCCTCTCCAC ACCACCACGC GCCCGCTCAC 
CCTGGAACTC ATAAACCTCC TCATCCCAGA GGGCCCAGAA AGGGCCTCCA TGAACCCTAC 
AGTGAGACTG ACGAGGATGA CTGGTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_017354800.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017210289.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X15, mRNA.

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CloneID	ODa58256
Clone ID Related Accession (Same CDS sequence)	XM_017354800.1
Accession Version	XM_017354800.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X15
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCTAAGGA GCAGCAGAAG AACCACAAAG GTTGTAAGTC GGTATGGGAG 
CAGCGCACTA GTGAGTTAAG ACGACAGACC CTCGTGAACA GCAGAGAGGC TCTTTATAAT 
GAGTTGGACC CTGAAGACCG TTGGAAGGTC TCATACTCCC GTCACATACG TCCCGACATG 
AAAACTCACC TGGACCGGCC GTTAGTCGTG GACCCTCAGG AGAACCGCAA TAACAACACT 
AACAAAACCC GTCCGGGTGA CGGCCAACCT CAGCATTCCC ATCAGCTCCT ACACAAACAG 
CCCAACTTCA ATGAGGCCGA GACTGGAGGA GGTGGAGGGT CTGAGTCCTG GCCCGCACTG 
GAAAGAGGAG ACTCAACAGG GTCCCGCCGC AGTCTTTTGG GCTCTGAGCA CTATGAGGAG 
GGTAGAGAGT CCCACCGCAG ACACCAGCAC AGCCAGCACT GCAGTCAGGC CCGTCAACAT 
CGCAAAGCTG CAGAGGGCGG TGGAGAGCCT GGAGGGAGGA GACACAGGAG TAAGGCCAAG 
GAAAGAGGAC CTGAGTGTGA GCATTCAGAT GGAGGGGAGC GGAAACACAG ACGGCATCGG 
CATGAGGGTG AGGGGAGGAA AGAGAGAGGG GTACGACACC GAAACAGGAG GGATGGCCAT 
GCAAGTGGCC CGACTCTCTC TACAACCCGT CCCATTCAGA AGACTCTGTC CAGGCAGGAC 
AGTCAGTACA GCGAAGACCT GGACAATGCC ATGAACAACA AACTTGCTAC CCAGCCACAT 
GATAGTCTGC TTAATCTGGC CAACACTGCC CACTCTGCCG GCCTGGCACA TACTGGTACA 
GAGAGCAGCC TCATCCTCAC CAACCCTTCC TCTACCATTG CAAATGTTAC TAGCATCGGT 
CACCTGGGTA TGAAACCTGA GTACACTGCC GTGGACATTC CTCCCATGTT CCCCTCCAGT 
AATGCCATTC TACAGGTCAA TAAGAATGCC AACACGGAGC CTCTGCCAAA GAAGGAAGAC 
ACAAAGGGTG ACGATGATGA TGATGAGAAA GATGACGGAG GGCCTAAACC CATGCCACCC 
TACACCTCAA TGTTCATTCT GACCACCACC AACCCATTTC GGAGATTGTG TCACTATATC 
GTCACACTGA GATATTTTGA GATGTGTATT CTACTGGTTA TTGCTATGAG CAGTATTGCC 
CTGGCTGCTG AAGACCCTGT CTGGCCTGAA TCTCCCCGCA ACAATGTCCT ACGATACTTT 
GACTATGTGT TCACTGGTGT GTTCACCTTT GAGATGCTCA TTAAGATGGT AGATTTAGGT 
CTTGTCCTGC ATCAAGGCTC ATACTTTCGT GATCTGTGGA ACATCCTGGA CTTTATAGTG 
GTTAGTGGTG CCCTGGTGGC GTTCGCCTTC ACCGGAGGCA GCGGAGGAAG CAGCAAAGGG 
AAAGACATCA GTACTATCAA GTCTCTACGT GTGCTTCGAG TACTGCGGCC GCTGAAAACC 
ATCAAACGTC TGCCAAAACT GAAGGCTGTC TTTGACTGTG TGGTGAACTC TCTGAAGAAT 
GTGTTGAACA TCCTCATCGT CTACATGCTC TTCATGTTCA TCTTCGCTGT GGTGGCTGTG 
CAGCTCTTTA AGGGACGTTT CTTTTACTGC ACTGATGAAT CCAAAGAGTT TGAGCGCGAC 
TGCAGGGGCG AGTATCTGGT CTATGAAAGA GATAACGAAG TGAGGTCACA GAAGCGGGAA 
TGGAAGAAAT ACGATTTCCA CTACGACAAC GTGCTCTGGG CCCTTCTCAC CCTCTTCACC 
GTTTCTACGG GAGAGGGGTG GCCGCAAGTG CTAAAACATT CAGTAGATGC GACCTACGAG 
AATCAGGGCC CGAGCCCAGG TTACCGGATG GAAATGTCCA TCTTTTACGT GGTGTACTTC 
GTGGTCTTCC CCTTCTTCTT CGTCAACATC TTCGTGGCTC TTATCATCAT TACTTTCCAG 
GAGCAAGGAG ACAAAATGAT GGAGGACTAT AGCCTGGAAA AGAATGAGAG AGCCTGCATA 
GACTTTGCCA TCAACGCTAA GCCGCTCACA CGGCACATGC CCCAGAACAA GCAGACCTTT 
CAGTACCGCA TGTGGGAGTT TGTGGTGTCT CCTCCATTTG AATACACCAT CATGGCCTTG 
ATCGCGCTCA ACACTATTGT CCTTATGATG AAGTATGATG GAGCATCGCT TACTTATGAA 
GATGTGCTAA AATACCTGAA CATAGTGTTC ACCTCTCTAT TCTCCATGGA GTGCATATTG 
AAGATCATAG CCTTCGGAGC ATTGAACTAC TTCAAAGATG CCTGGAACAT CTTCGACTGT 
GTGACTGTAC TTGGCAGCAT CACTGATATT TTAGTCACAG AGCTGGGGAT GAACAACTTC 
ATAAATCTGA GTTTTCTAAG GCTCTTCAGA GCTGCTCGTC TCATTAAGTT GCTCAGGCAG 
GGTGAAACCA TACGCATCCT GCTCTGGACT TTTGTCCAGT CTTTCAAGGC TCTGCCGTAT 
GTGTGCCTAC TCATCGCCAT GCTGTTCTTC ATCTACGCCA TCATTGGCAT GCAGCTGTTT 
GGGAACATTA AGATCGAGGA GAACAGTGAC AGTGCCATTA CCCAGCACAA CAACTTCAGG 
ACTTTTTTCC AGGCCCTAAT GCTGCTCTTT AGGAGTGCTA CAGGTGAGGC ATGGCATGAC 
ATCATGCTGT CATGTCTGGG AAAGAAGCCG TGTGATATTC TCTCTGATAA CCCGAAGCCA 
GAATGCGGGA GCGAGTTTGC CTACCTCTAC TTTGTCTCCT TCATCTTCCT CTGTTCATTT 
CTGATGCTGA ATCTGTTCGT GGCTGTGATC ATGGATAATT TTGAATACCT GACGCGTGAC 
TCCTCTATCC TGGGACCCCA TCACCTGGAC GAGTATGTGA GGATCTGGGC AGAATATGAT 
CCTGCTGCTT GCGGGCGCAT TCATTATAAG GATATGTACA GTTTATTGCG AGTTATCGAC 
CCCCCTCTGG GCTTAGGCAA GAAATGTCCT CATAGGGTGG CCTGCAAGAG ACTGTTGCGG 
ATGGATCTGC CGGTTGCCGA TGACAACACA GTGCACTTTA ATTCTACCCT TATGGCCTTA 
ATCCGAACCG CTCTGGACAT CAAGATCGCC AAGGGTGGGG AAGGAGGTGT AGATAAGCAC 
CAGATGGATG CTGAATTAAG GAAGGAGATG ATGGCGATAT GGCCTAATCT CTCTCAGAAG 
AACCTGGACC TGCTGGTGAC TCCTCACAAG TCAGCGACAG ACCTGACAGT AGGAAAGATC 
TACGCTGCCA TGATGATCAT GGAGTATTAT CGGCAAAGTA AAGCCAAACG GACACAAGCT 
CTCCATGATG AGCAGAATCG AACGCCATTA ATGTTTCAGC GTCTGGAGCC GCCATCTCCT 
AGCCAGGATG TGGGACCAGG ACTCACTGGC CTTCCTGACA CACAAATGCA CCCTGCAAAT 
CATCTGCCTG TTGATGAAAG GATCCCAGAG AGCCAATCAT GGGTGACAGC GCGAGCACAG 
GAGATTTCTC AAAAAGCAGG CAACTGGAGT CCAGAGGGCC AACATCCAGA TGACACCACA 
GACAACAGAA GGCAGTCTCA GACAGTAGAG ATGAGAGAGA TGGGGCGGGA CGGGTACTCG 
GACACAGAGC AATATCTGCC GATGGAAGGC CATGGCAGGG CTGCTTCCAT GCCTCGACTC 
CCAGCAGACA ACCAACAGCC TATCACAGAT AACAGTCCTA TGAAGCGCTC GGCTTCATCT 
TTGGGACACG GGCGTGCAGG ACGCTCCATG CGAGGTGAGG ATTATGCCAT GGAGAGGGTT 
ATCCCAGAAG AGGGTCACAG GCATGGGCAT CGCCACCGAG ACCGTAGTCA CCGGGCGTCA 
GAACGCTCCC TGAGCCGATA CACTGATGCT GACACAGGTC TTGGAACTGA CCTCAGCACA 
ACCACACAAT CAGGAGACCT GCCATCCAAG GAGAGAGACC GGGGCCGAGC CAAAGACCGC 
AAGCACCATC ATCATCATCA TCACCACCAC GGCTCTTTAG ATAAGGAGCA CTACGGTCAT 
GAGAGAGACA GAGGAGAGTA CGGCCACAGA CAGTCCAGAG AGAGAGACCG CCGCTGGTCC 
CGCTCACCCA GTGAGGGCAG AGAGTGTCTG ACTCACAGAC AGGGCAGTAG TTCAGTAAGC 
GGCAGTCCAG TCCCGTCCAC CTCCGGGACC AGCACCCCCC GCCGTGGCCG CCGTCAGCTC 
CCCCAAACCC CTGCTACACC CCGGCCACAT GTCACCTACT CACCTGTGGT CCGTAAGCCC 
ATCAGCAGTA CCCCCCCTCC TGGCCAGCAG AGCCGACTCC CTACCCCGAC CTCCCGGCGC 
TTCAGTCCGA CTGGCCCCGA GCCCCCGCTT CCCCCTCATC ACCACCCGTC ACCACCCCAC 
CACGGCTCTC CCCGCTCCGG CCGACATGCA CACTGGGGCC CCGAGCCTGC AGAGAGCGTG 
GAGGGTGACG GCTTCTATGA CGACCAAGAC TATGAGTTCA ATCACCACGA GCCTCCATCC 
TACGAGCAAA GCCCTGTGCA GGGCAACCCT CACCCCCATT CCCCTAGGAC TTCCCGCCAC 
AACACCCCTC CTCAGGGCCA AGCGCACCCA CGGCGCATGC CAAATGGCTA CCGTTCGTCT 
TCGCCCTCTC CACACCACCA CGCGCCCGCT CACCCTGGAA CTCATAAACC TCCTCATCCC 
AGAGGGCCCA GAAAGGGCCT CCATGAACCC TACAGTGAGA CTGACGAGGA TGACTGGTGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210289.1
CDS	1466..8488
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGGSGGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGMNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGEGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQNRTPLMFQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQTVEMREMGRDGYSDTEQYLPMEGHGRAASMPRLPADNQQPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQGSSSVSGSPVPSTSGTSTPRRGRRQLPQTPATPRPHVTYSPVVRKPISSTPPPGQQSRLPTPTSRRFSPTGPEPPLPPHHHPSPPHHGSPRSGRHAHWGPEPAESVEGDGFYDDQDYEFNHHEPPSYEQSPVQGNPHPHSPRTSRHNTPPQGQAHPRRMPNGYRSSSPSPHHHAPAHPGTHKPPHPRGPRKGLHEPYSETDEDDWC

Target ORF information:

RefSeq Version	XM_017354800.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354800.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCTAAGGA GCAGCAGAAG AACCACAAAG GTTGTAAGTC GGTATGGGAG 
CAGCGCACTA GTGAGTTAAG ACGACAGACC CTCGTGAACA GCAGAGAGGC TCTTTATAAT 
GAGTTGGACC CTGAAGACCG TTGGAAGGTC TCATACTCCC GTCACATACG TCCCGACATG 
AAAACTCACC TGGACCGGCC GTTAGTCGTG GACCCTCAGG AGAACCGCAA TAACAACACT 
AACAAAACCC GTCCGGGTGA CGGCCAACCT CAGCATTCCC ATCAGCTCCT ACACAAACAG 
CCCAACTTCA ATGAGGCCGA GACTGGAGGA GGTGGAGGGT CTGAGTCCTG GCCCGCACTG 
GAAAGAGGAG ACTCAACAGG GTCCCGCCGC AGTCTTTTGG GCTCTGAGCA CTATGAGGAG 
GGTAGAGAGT CCCACCGCAG ACACCAGCAC AGCCAGCACT GCAGTCAGGC CCGTCAACAT 
CGCAAAGCTG CAGAGGGCGG TGGAGAGCCT GGAGGGAGGA GACACAGGAG TAAGGCCAAG 
GAAAGAGGAC CTGAGTGTGA GCATTCAGAT GGAGGGGAGC GGAAACACAG ACGGCATCGG 
CATGAGGGTG AGGGGAGGAA AGAGAGAGGG GTACGACACC GAAACAGGAG GGATGGCCAT 
GCAAGTGGCC CGACTCTCTC TACAACCCGT CCCATTCAGA AGACTCTGTC CAGGCAGGAC 
AGTCAGTACA GCGAAGACCT GGACAATGCC ATGAACAACA AACTTGCTAC CCAGCCACAT 
GATAGTCTGC TTAATCTGGC CAACACTGCC CACTCTGCCG GCCTGGCACA TACTGGTACA 
GAGAGCAGCC TCATCCTCAC CAACCCTTCC TCTACCATTG CAAATGTTAC TAGCATCGGT 
CACCTGGGTA TGAAACCTGA GTACACTGCC GTGGACATTC CTCCCATGTT CCCCTCCAGT 
AATGCCATTC TACAGGTCAA TAAGAATGCC AACACGGAGC CTCTGCCAAA GAAGGAAGAC 
ACAAAGGGTG ACGATGATGA TGATGAGAAA GATGACGGAG GGCCTAAACC CATGCCACCC 
TACACCTCAA TGTTCATTCT GACCACCACC AACCCATTTC GGAGATTGTG TCACTATATC 
GTCACACTGA GATATTTTGA GATGTGTATT CTACTGGTTA TTGCTATGAG CAGTATTGCC 
CTGGCTGCTG AAGACCCTGT CTGGCCTGAA TCTCCCCGCA ACAATGTCCT ACGATACTTT 
GACTATGTGT TCACTGGTGT GTTCACCTTT GAGATGCTCA TTAAGATGGT AGATTTAGGT 
CTTGTCCTGC ATCAAGGCTC ATACTTTCGT GATCTGTGGA ACATCCTGGA CTTTATAGTG 
GTTAGTGGTG CCCTGGTGGC GTTCGCCTTC ACCGGAGGCA GCGGAGGAAG CAGCAAAGGG 
AAAGACATCA GTACTATCAA GTCTCTACGT GTGCTTCGAG TACTGCGGCC GCTGAAAACC 
ATCAAACGTC TGCCAAAACT GAAGGCTGTC TTTGACTGTG TGGTGAACTC TCTGAAGAAT 
GTGTTGAACA TCCTCATCGT CTACATGCTC TTCATGTTCA TCTTCGCTGT GGTGGCTGTG 
CAGCTCTTTA AGGGACGTTT CTTTTACTGC ACTGATGAAT CCAAAGAGTT TGAGCGCGAC 
TGCAGGGGCG AGTATCTGGT CTATGAAAGA GATAACGAAG TGAGGTCACA GAAGCGGGAA 
TGGAAGAAAT ACGATTTCCA CTACGACAAC GTGCTCTGGG CCCTTCTCAC CCTCTTCACC 
GTTTCTACGG GAGAGGGGTG GCCGCAAGTG CTAAAACATT CAGTAGATGC GACCTACGAG 
AATCAGGGCC CGAGCCCAGG TTACCGGATG GAAATGTCCA TCTTTTACGT GGTGTACTTC 
GTGGTCTTCC CCTTCTTCTT CGTCAACATC TTCGTGGCTC TTATCATCAT TACTTTCCAG 
GAGCAAGGAG ACAAAATGAT GGAGGACTAT AGCCTGGAAA AGAATGAGAG AGCCTGCATA 
GACTTTGCCA TCAACGCTAA GCCGCTCACA CGGCACATGC CCCAGAACAA GCAGACCTTT 
CAGTACCGCA TGTGGGAGTT TGTGGTGTCT CCTCCATTTG AATACACCAT CATGGCCTTG 
ATCGCGCTCA ACACTATTGT CCTTATGATG AAGTATGATG GAGCATCGCT TACTTATGAA 
GATGTGCTAA AATACCTGAA CATAGTGTTC ACCTCTCTAT TCTCCATGGA GTGCATATTG 
AAGATCATAG CCTTCGGAGC ATTGAACTAC TTCAAAGATG CCTGGAACAT CTTCGACTGT 
GTGACTGTAC TTGGCAGCAT CACTGATATT TTAGTCACAG AGCTGGGGAT GAACAACTTC 
ATAAATCTGA GTTTTCTAAG GCTCTTCAGA GCTGCTCGTC TCATTAAGTT GCTCAGGCAG 
GGTGAAACCA TACGCATCCT GCTCTGGACT TTTGTCCAGT CTTTCAAGGC TCTGCCGTAT 
GTGTGCCTAC TCATCGCCAT GCTGTTCTTC ATCTACGCCA TCATTGGCAT GCAGCTGTTT 
GGGAACATTA AGATCGAGGA GAACAGTGAC AGTGCCATTA CCCAGCACAA CAACTTCAGG 
ACTTTTTTCC AGGCCCTAAT GCTGCTCTTT AGGAGTGCTA CAGGTGAGGC ATGGCATGAC 
ATCATGCTGT CATGTCTGGG AAAGAAGCCG TGTGATATTC TCTCTGATAA CCCGAAGCCA 
GAATGCGGGA GCGAGTTTGC CTACCTCTAC TTTGTCTCCT TCATCTTCCT CTGTTCATTT 
CTGATGCTGA ATCTGTTCGT GGCTGTGATC ATGGATAATT TTGAATACCT GACGCGTGAC 
TCCTCTATCC TGGGACCCCA TCACCTGGAC GAGTATGTGA GGATCTGGGC AGAATATGAT 
CCTGCTGCTT GCGGGCGCAT TCATTATAAG GATATGTACA GTTTATTGCG AGTTATCGAC 
CCCCCTCTGG GCTTAGGCAA GAAATGTCCT CATAGGGTGG CCTGCAAGAG ACTGTTGCGG 
ATGGATCTGC CGGTTGCCGA TGACAACACA GTGCACTTTA ATTCTACCCT TATGGCCTTA 
ATCCGAACCG CTCTGGACAT CAAGATCGCC AAGGGTGGGG AAGGAGGTGT AGATAAGCAC 
CAGATGGATG CTGAATTAAG GAAGGAGATG ATGGCGATAT GGCCTAATCT CTCTCAGAAG 
AACCTGGACC TGCTGGTGAC TCCTCACAAG TCAGCGACAG ACCTGACAGT AGGAAAGATC 
TACGCTGCCA TGATGATCAT GGAGTATTAT CGGCAAAGTA AAGCCAAACG GACACAAGCT 
CTCCATGATG AGCAGAATCG AACGCCATTA ATGTTTCAGC GTCTGGAGCC GCCATCTCCT 
AGCCAGGATG TGGGACCAGG ACTCACTGGC CTTCCTGACA CACAAATGCA CCCTGCAAAT 
CATCTGCCTG TTGATGAAAG GATCCCAGAG AGCCAATCAT GGGTGACAGC GCGAGCACAG 
GAGATTTCTC AAAAAGCAGG CAACTGGAGT CCAGAGGGCC AACATCCAGA TGACACCACA 
GACAACAGAA GGCAGTCTCA GACAGTAGAG ATGAGAGAGA TGGGGCGGGA CGGGTACTCG 
GACACAGAGC AATATCTGCC GATGGAAGGC CATGGCAGGG CTGCTTCCAT GCCTCGACTC 
CCAGCAGACA ACCAACAGCC TATCACAGAT AACAGTCCTA TGAAGCGCTC GGCTTCATCT 
TTGGGACACG GGCGTGCAGG ACGCTCCATG CGAGGTGAGG ATTATGCCAT GGAGAGGGTT 
ATCCCAGAAG AGGGTCACAG GCATGGGCAT CGCCACCGAG ACCGTAGTCA CCGGGCGTCA 
GAACGCTCCC TGAGCCGATA CACTGATGCT GACACAGGTC TTGGAACTGA CCTCAGCACA 
ACCACACAAT CAGGAGACCT GCCATCCAAG GAGAGAGACC GGGGCCGAGC CAAAGACCGC 
AAGCACCATC ATCATCATCA TCACCACCAC GGCTCTTTAG ATAAGGAGCA CTACGGTCAT 
GAGAGAGACA GAGGAGAGTA CGGCCACAGA CAGTCCAGAG AGAGAGACCG CCGCTGGTCC 
CGCTCACCCA GTGAGGGCAG AGAGTGTCTG ACTCACAGAC AGGGCAGTAG TTCAGTAAGC 
GGCAGTCCAG TCCCGTCCAC CTCCGGGACC AGCACCCCCC GCCGTGGCCG CCGTCAGCTC 
CCCCAAACCC CTGCTACACC CCGGCCACAT GTCACCTACT CACCTGTGGT CCGTAAGCCC 
ATCAGCAGTA CCCCCCCTCC TGGCCAGCAG AGCCGACTCC CTACCCCGAC CTCCCGGCGC 
TTCAGTCCGA CTGGCCCCGA GCCCCCGCTT CCCCCTCATC ACCACCCGTC ACCACCCCAC 
CACGGCTCTC CCCGCTCCGG CCGACATGCA CACTGGGGCC CCGAGCCTGC AGAGAGCGTG 
GAGGGTGACG GCTTCTATGA CGACCAAGAC TATGAGTTCA ATCACCACGA GCCTCCATCC 
TACGAGCAAA GCCCTGTGCA GGGCAACCCT CACCCCCATT CCCCTAGGAC TTCCCGCCAC 
AACACCCCTC CTCAGGGCCA AGCGCACCCA CGGCGCATGC CAAATGGCTA CCGTTCGTCT 
TCGCCCTCTC CACACCACCA CGCGCCCGCT CACCCTGGAA CTCATAAACC TCCTCATCCC 
AGAGGGCCCA GAAAGGGCCT CCATGAACCC TACAGTGAGA CTGACGAGGA TGACTGGTGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1aa ( XM_017354801.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017210290.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X18, mRNA.

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CloneID	ODa58257
Clone ID Related Accession (Same CDS sequence)	XM_017354801.1
Accession Version	XM_017354801.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X18
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG 
GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT 
GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC 
CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC 
ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT 
GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT 
GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT 
GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT 
CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA 
AGGCAGTCTC AGACAGTAGA GATGAGAGAG ATGGGGCGGG ACGGGTACTC GGACACAGAG 
CAATATCTGC CGATGGAAGG CCATGGCAGG GCTGCTTCCA TGCCTCGACT CCCAGCAGAC 
AACCAACAGC CTATCACAGA TAACAGTCCT ATGAAGCGCT CGGCTTCATC TTTGGGACAC 
GGGCGTGCAG GACGCTCCAT GCGAGGTGAG GATTATGCCA TGGAGAGGGT TATCCCAGAA 
GAGGGTCACA GGCATGGGCA TCGCCACCGA GACCGTAGTC ACCGGGCGTC AGAACGCTCC 
CTGAGCCGAT ACACTGATGC TGACACAGGT CTTGGAACTG ACCTCAGCAC AACCACACAA 
TCAGGAGACC TGCCATCCAA GGAGAGAGAC CGGGGCCGAG CCAAAGACCG CAAGCACCAT 
CATCATCATC ATCACCACCA CGGCTCTTTA GATAAGGAGC ACTACGGTCA TGAGAGAGAC 
AGAGGAGAGT ACGGCCACAG ACAGTCCAGA GAGAGAGACC GCCGCTGGTC CCGCTCACCC 
AGTGAGGGCA GAGAGTGTCT GACTCACAGA CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210290.1
CDS	1474..7929
Translation	MARFGDEVPSRYGGGPGHAQGGPGRGGSRQGGPPGAQQRMYKQSMAQRARTMALYNPIPVRQNCFTVNRSLFIFSEDNFVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLEDTEPYFIGIFCFESGIKILALGFAFHKGSYLRNGWNVMDFVVVLTGILSTVGSDFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILMFAIIGLEFYMGKFHTTCFDKITDEIREEFPCGEEVPARICPNGTVCKKYWLGPNYGITQFDNILFAVLTVFQCITMEGWTDLLYYSNDAAGSAWNWMYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRSEFLKLKRQQQIERELNGYLEWICKAEEVILADEDNDPDDRMPFDGSRRRPTIKKSKTDLLDAEDGDGDIGSPFARGSLKSSKLEGSSFHKKERRLRFFIRRIVKTQAFYWTVLCLVGLNTLCVAVVHYDQPETLSDFLYFAEFIFLGIFMSEMCIKMYGLGTRPYFHSSFNCFDCIVICGSIFEVLWAMIQPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEAGTPPTNFDTFPAAIMTVFQILTGEDWNMVMYDGIESQGGVKKGMVFSVFFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEQAANKKMALQTAKEVAEVSPLSAANLSIAAVTVNSERLDATEHFLDCKEQQKNHKGCKSVWEQRTSELRRQTLVNSREALYNELDPEDRWKVSYSRHIRPDMKTHLDRPLVVDPQENRNNNTNKTRPGDGQPQHSHQLLHKQPNFNEAETGGGGGSESWPALERGDSTGSRRSLLGSEHYEEGRESHRRHQHSQHCSQARQHRKAAEGGGEPGGRRHRSKAKERGPECEHSDGGERKHRRHRHEGEGRKERGVRHRNRRDGHASGPTLSTTRPIQKTLSRQDSQYSEDLDNAMNNKLATQPHDSLLNLANTAHSAGLAHTGTESSLILTNPSSTIANVTSIGHLGMKPEYTAVDIPPMFPSSNAILQVNKNANTEPLPKKEDTKGDDDDDEKDDGGPKPMPPYTSMFILTTTNPFRRLCHYIVTLRYFEMCILLVIAMSSIALAAEDPVWPESPRNNVLRYFDYVFTGVFTFEMLIKMVDLGLVLHQGSYFRDLWNILDFIVVSGALVAFAFTGGSGGSSKGKDISTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVVAVQLFKGRFFYCTDESKEFERDCRGEYLVYERDNEVRSQKREWKKYDFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATYENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEDYSLEKNERACIDFAINAKPLTRHMPQNKQTFQYRMWEFVVSPPFEYTIMALIALNTIVLMMKYDGASLTYEDVLKYLNIVFTSLFSMECILKIIAFGALNYFKDAWNIFDCVTVLGSITDILVTELGMNNFINLSFLRLFRAARLIKLLRQGETIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQLFGNIKIEENSDSAITQHNNFRTFFQALMLLFRSATGEAWHDIMLSCLGKKPCDILSDNPKPECGSEFAYLYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRIWAEYDPAACGRIHYKDMYSLLRVIDPPLGLGKKCPHRVACKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGEGGVDKHQMDAELRKEMMAIWPNLSQKNLDLLVTPHKSATDLTVGKIYAAMMIMEYYRQSKAKRTQALHDEQNRTPLMFQRLEPPSPSQDVGPGLTGLPDTQMHPANHLPVDERIPESQSWVTARAQEISQKAGNWSPEGQHPDDTTDNRRQSQTVEMREMGRDGYSDTEQYLPMEGHGRAASMPRLPADNQQPITDNSPMKRSASSLGHGRAGRSMRGEDYAMERVIPEEGHRHGHRHRDRSHRASERSLSRYTDADTGLGTDLSTTTQSGDLPSKERDRGRAKDRKHHHHHHHHHGSLDKEHYGHERDRGEYGHRQSRERDRRWSRSPSEGRECLTHRQ

Target ORF information:

RefSeq Version	XM_017354801.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354801.1

ORF Insert Sequence:

ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG 
TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG 
CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG 
AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC 
ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA 
CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG 
TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT 
GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA 
GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT 
GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT 
CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC 
TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG 
TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG 
TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC 
ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT 
GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC 
TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT 
GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG 
AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT 
GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT 
AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG 
GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA 
AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG 
GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT 
GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT 
AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC 
ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA 
TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT 
TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT 
TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT 
GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA 
GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT 
GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT 
CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC 
CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG 
AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC 
CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA 
GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT 
AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC 
CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC 
CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC 
CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC 
AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA 
GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG 
TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT 
GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA 
CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT 
GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC 
CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC 
AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG 
CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC 
CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT 
ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT 
CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT 
GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA 
ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG 
AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT 
GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG 
TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG 
CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT 
GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC 
AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT 
CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC 
ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT 
AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC 
GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA 
TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG 
GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC 
CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC 
CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA 
GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC 
ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC 
ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC 
AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA 
AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA 
GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA 
CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG 
AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC 
ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA 
CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT 
AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC 
CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG 
TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG 
AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG 
AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC 
CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT 
TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG 
GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG 
CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC 
GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT 
GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC 
CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC 
ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT 
GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT 
GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT 
GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT 
CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA 
AGGCAGTCTC AGACAGTAGA GATGAGAGAG ATGGGGCGGG ACGGGTACTC GGACACAGAG 
CAATATCTGC CGATGGAAGG CCATGGCAGG GCTGCTTCCA TGCCTCGACT CCCAGCAGAC 
AACCAACAGC CTATCACAGA TAACAGTCCT ATGAAGCGCT CGGCTTCATC TTTGGGACAC 
GGGCGTGCAG GACGCTCCAT GCGAGGTGAG GATTATGCCA TGGAGAGGGT TATCCCAGAA 
GAGGGTCACA GGCATGGGCA TCGCCACCGA GACCGTAGTC ACCGGGCGTC AGAACGCTCC 
CTGAGCCGAT ACACTGATGC TGACACAGGT CTTGGAACTG ACCTCAGCAC AACCACACAA 
TCAGGAGACC TGCCATCCAA GGAGAGAGAC CGGGGCCGAG CCAAAGACCG CAAGCACCAT 
CATCATCATC ATCACCACCA CGGCTCTTTA GATAAGGAGC ACTACGGTCA TGAGAGAGAC 
AGAGGAGAGT ACGGCCACAG ACAGTCCAGA GAGAGAGACC GCCGCTGGTC CCGCTCACCC 
AGTGAGGGCA GAGAGTGTCT GACTCACAGA CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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見積依頼

Eメール

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03-6811-6572

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03-6811-6573
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オンライン注文

cacna1aa cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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