cacna1aa cDNA ORF clone, Danio rerio(zebrafish)

The following cacna1aa gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cacna1aa cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa08534 XM_005164090.4
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
ODa141783 XM_021474372.1
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa141784 XM_021474375.1
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa141785 XM_021474379.1
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa141786 XM_021474381.1
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa141787 XM_021474386.1
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa141788 XM_021474390.1
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa141789 XM_021474393.1
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa141790 XM_021474394.1
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa141791 XM_021474398.1
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa141792 XM_021474403.1
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa141793 XM_021474404.1
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa141794 XM_021474408.1
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa141795 XM_021474412.1
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa141796 XM_021474420.1
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa141797 XM_021474422.1
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa141798 XM_021474423.1
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa141799 XM_021474428.1
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa141800 XM_021474432.1
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa141801 XM_021474433.1
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa141802 XM_021474440.1
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa141803 XM_021474441.1
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X27, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa141804 XM_021474442.1
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X28, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa43660 XM_009299585.3
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa43661 XM_009299586.2
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa43662 XM_009299587.2
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa43663 XM_009299588.2
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa43664 XM_009299589.2
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa43665 XM_009299590.3
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa43666 XM_009299591.3
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa43667 XM_009299592.2
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa43668 XM_009299593.3
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X26, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa58247 XM_017354791.1
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa58248 XM_017354792.1
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa58249 XM_017354793.1
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa58250 XM_017354794.2
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa58251 XM_017354795.1
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa58252 XM_017354796.1
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa58253 XM_017354797.1
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa58254 XM_017354798.1
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa58255 XM_017354799.1
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa58256 XM_017354800.1
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa58257 XM_017354801.1
Latest version!
Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
View Old Accession Versions >>
ODa43665 XM_009299590.2 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa58250 XM_017354794.1 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa43666 XM_009299591.2 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
ODa43660 XM_009299585.2 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1371.30
$1959.00
ODa08534 XM_005164090.3 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
ODa43668 XM_009299593.2 Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1161.30
$1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa08534
    Clone ID Related Accession (Same CDS sequence) XM_005164090.4 , XM_005164090.3
    Accession Version XM_005164090.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5223bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X24
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005164090.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005164147.1
    CDS1542..6764
    Translation

    Target ORF information:

    RefSeq Version XM_005164090.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005164090.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa08534
    Clone ID Related Accession (Same CDS sequence) XM_005164090.4 , XM_005164090.3
    Accession Version XM_005164090.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5223bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X19
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005164090.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005164147.1
    CDS1507..6729
    Translation

    Target ORF information:

    RefSeq Version XM_005164090.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005164090.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141783
    Clone ID Related Accession (Same CDS sequence) XM_021474372.1
    Accession Version XM_021474372.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7116bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG
    GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT
    GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC
    CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC
    ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT
    GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT
    GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT
    GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT
    CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA
    AGGCAGTCTC AGACAGTAGA GATGAGAGAG ATGGGGCGGG ACGGGTACTC GGACACAGAG
    CAATATCTGC CGATGGAAGG CCATGGCAGG GCTGCTTCCA TGCCTCGACT CCCAGCAGAC
    AACCAACAGC CACGGAGGAA AGGGCGTCAC CGGGGAGAAA ATCTCACTCA GCCTATCACA
    GATAACAGTC CTATGAAGCG CTCGGCTTCA TCTTTGGGAC ACGGGCGTGC AGGACGCTCC
    ATGCGAGGTG AGGATTATGC CATGGAGAGG GTTATCCCAG AAGAGGGTCA CAGGCATGGG
    CATCGCCACC GAGACCGTAG TCACCGGGCG TCAGAACGCT CCCTGAGCCG ATACACTGAT
    GCTGACACAG GTCTTGGAAC TGACCTCAGC ACAACCACAC AATCAGGAGA CCTGCCATCC
    AAGGAGAGAG ACCGGGGCCG AGCCAAAGAC CGCAAGCACC ATCATCATCA TCATCACCAC
    CACGGCTCTT TAGATAAGGA GCACTACGGT CATGAGAGAG ACAGAGGAGA GTACGGCCAC
    AGACAGTCCA GAGAGAGAGA CCGCCGCTGG TCCCGCTCAC CCAGTGAGGG CAGAGAGTGT
    CTGACTCACA GACAGGGCAG TAGTTCAGTA AGCGGCAGTC CAGTCCCGTC CACCTCCGGG
    ACCAGCACCC CCCGCCGTGG CCGCCGTCAG CTCCCCCAAA CCCCTGCTAC ACCCCGGCCA
    CATGTCACCT ACTCACCTGT GGTCCGTAAG CCCATCAGCA GTACCCCCCC TCCTGGCCAG
    CAGAGCCGAC TCCCTACCCC GACCTCCCGG CGCTTCAGTC CGACTGGCCC CGAGCCCCCG
    CTTCCCCCTC ATCACCACCC GTCACCACCC CACCACGGCT CTCCCCGCTC CGGCCGACAT
    GCACACTGGG GCCCCGAGCC TGCAGAGAGC GTGGAGGGTG ACGGCTTCTA TGACGACCAA
    GACTATGAGT TCAATCACCA CGAGCCTCCA TCCTACGAGC AAAGCCCTGT GCAGGGCAAC
    CCTCACCCCC ATTCCCCTAG GACTTCCCGC CACAACACCC CTCCTCAGGG CCAAGCGCAC
    CCACGGCGCA TGCCAAATGG CTACCGTTCG TCTTCGCCCT CTCCACACCA CCACGCGCCC
    GCTCACCCTG GAACTCATAA ACCTCCTCAT CCCAGAGGGC CCAGAAAGGG CCTCCATGAA
    CCCTACAGTG AGACTGACGA GGATGACTGG TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330047.1
    CDS1507..8622
    Translation

    Target ORF information:

    RefSeq Version XM_021474372.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474372.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG
    GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT
    GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC
    CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC
    ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT
    GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT
    GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT
    GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT
    CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA
    AGGCAGTCTC AGACAGTAGA GATGAGAGAG ATGGGGCGGG ACGGGTACTC GGACACAGAG
    CAATATCTGC CGATGGAAGG CCATGGCAGG GCTGCTTCCA TGCCTCGACT CCCAGCAGAC
    AACCAACAGC CACGGAGGAA AGGGCGTCAC CGGGGAGAAA ATCTCACTCA GCCTATCACA
    GATAACAGTC CTATGAAGCG CTCGGCTTCA TCTTTGGGAC ACGGGCGTGC AGGACGCTCC
    ATGCGAGGTG AGGATTATGC CATGGAGAGG GTTATCCCAG AAGAGGGTCA CAGGCATGGG
    CATCGCCACC GAGACCGTAG TCACCGGGCG TCAGAACGCT CCCTGAGCCG ATACACTGAT
    GCTGACACAG GTCTTGGAAC TGACCTCAGC ACAACCACAC AATCAGGAGA CCTGCCATCC
    AAGGAGAGAG ACCGGGGCCG AGCCAAAGAC CGCAAGCACC ATCATCATCA TCATCACCAC
    CACGGCTCTT TAGATAAGGA GCACTACGGT CATGAGAGAG ACAGAGGAGA GTACGGCCAC
    AGACAGTCCA GAGAGAGAGA CCGCCGCTGG TCCCGCTCAC CCAGTGAGGG CAGAGAGTGT
    CTGACTCACA GACAGGGCAG TAGTTCAGTA AGCGGCAGTC CAGTCCCGTC CACCTCCGGG
    ACCAGCACCC CCCGCCGTGG CCGCCGTCAG CTCCCCCAAA CCCCTGCTAC ACCCCGGCCA
    CATGTCACCT ACTCACCTGT GGTCCGTAAG CCCATCAGCA GTACCCCCCC TCCTGGCCAG
    CAGAGCCGAC TCCCTACCCC GACCTCCCGG CGCTTCAGTC CGACTGGCCC CGAGCCCCCG
    CTTCCCCCTC ATCACCACCC GTCACCACCC CACCACGGCT CTCCCCGCTC CGGCCGACAT
    GCACACTGGG GCCCCGAGCC TGCAGAGAGC GTGGAGGGTG ACGGCTTCTA TGACGACCAA
    GACTATGAGT TCAATCACCA CGAGCCTCCA TCCTACGAGC AAAGCCCTGT GCAGGGCAAC
    CCTCACCCCC ATTCCCCTAG GACTTCCCGC CACAACACCC CTCCTCAGGG CCAAGCGCAC
    CCACGGCGCA TGCCAAATGG CTACCGTTCG TCTTCGCCCT CTCCACACCA CCACGCGCCC
    GCTCACCCTG GAACTCATAA ACCTCCTCAT CCCAGAGGGC CCAGAAAGGG CCTCCATGAA
    CCCTACAGTG AGACTGACGA GGATGACTGG TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141784
    Clone ID Related Accession (Same CDS sequence) XM_021474375.1
    Accession Version XM_021474375.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7116bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGTGGCAGAA TCAGTTACAG GGATATGTAT GAGATGCTCA GGCACATGTC TCCTCCGTTA
    GGCCTGGGGA AGAAGTGCCC TGCACGGGTT GCCTACAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT
    GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC
    CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC
    ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT
    GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT
    GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT
    GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT
    CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA
    AGGCAGTCTC AGACAGTAGA GATGAGAGAG ATGGGGCGGG ACGGGTACTC GGACACAGAG
    CAATATCTGC CGATGGAAGG CCATGGCAGG GCTGCTTCCA TGCCTCGACT CCCAGCAGAC
    AACCAACAGC CACGGAGGAA AGGGCGTCAC CGGGGAGAAA ATCTCACTCA GCCTATCACA
    GATAACAGTC CTATGAAGCG CTCGGCTTCA TCTTTGGGAC ACGGGCGTGC AGGACGCTCC
    ATGCGAGGTG AGGATTATGC CATGGAGAGG GTTATCCCAG AAGAGGGTCA CAGGCATGGG
    CATCGCCACC GAGACCGTAG TCACCGGGCG TCAGAACGCT CCCTGAGCCG ATACACTGAT
    GCTGACACAG GTCTTGGAAC TGACCTCAGC ACAACCACAC AATCAGGAGA CCTGCCATCC
    AAGGAGAGAG ACCGGGGCCG AGCCAAAGAC CGCAAGCACC ATCATCATCA TCATCACCAC
    CACGGCTCTT TAGATAAGGA GCACTACGGT CATGAGAGAG ACAGAGGAGA GTACGGCCAC
    AGACAGTCCA GAGAGAGAGA CCGCCGCTGG TCCCGCTCAC CCAGTGAGGG CAGAGAGTGT
    CTGACTCACA GACAGGGCAG TAGTTCAGTA AGCGGCAGTC CAGTCCCGTC CACCTCCGGG
    ACCAGCACCC CCCGCCGTGG CCGCCGTCAG CTCCCCCAAA CCCCTGCTAC ACCCCGGCCA
    CATGTCACCT ACTCACCTGT GGTCCGTAAG CCCATCAGCA GTACCCCCCC TCCTGGCCAG
    CAGAGCCGAC TCCCTACCCC GACCTCCCGG CGCTTCAGTC CGACTGGCCC CGAGCCCCCG
    CTTCCCCCTC ATCACCACCC GTCACCACCC CACCACGGCT CTCCCCGCTC CGGCCGACAT
    GCACACTGGG GCCCCGAGCC TGCAGAGAGC GTGGAGGGTG ACGGCTTCTA TGACGACCAA
    GACTATGAGT TCAATCACCA CGAGCCTCCA TCCTACGAGC AAAGCCCTGT GCAGGGCAAC
    CCTCACCCCC ATTCCCCTAG GACTTCCCGC CACAACACCC CTCCTCAGGG CCAAGCGCAC
    CCACGGCGCA TGCCAAATGG CTACCGTTCG TCTTCGCCCT CTCCACACCA CCACGCGCCC
    GCTCACCCTG GAACTCATAA ACCTCCTCAT CCCAGAGGGC CCAGAAAGGG CCTCCATGAA
    CCCTACAGTG AGACTGACGA GGATGACTGG TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330050.1
    CDS1508..8623
    Translation

    Target ORF information:

    RefSeq Version XM_021474375.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474375.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGTGGCAGAA TCAGTTACAG GGATATGTAT GAGATGCTCA GGCACATGTC TCCTCCGTTA
    GGCCTGGGGA AGAAGTGCCC TGCACGGGTT GCCTACAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT
    GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC
    CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC
    ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT
    GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT
    GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT
    GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT
    CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA
    AGGCAGTCTC AGACAGTAGA GATGAGAGAG ATGGGGCGGG ACGGGTACTC GGACACAGAG
    CAATATCTGC CGATGGAAGG CCATGGCAGG GCTGCTTCCA TGCCTCGACT CCCAGCAGAC
    AACCAACAGC CACGGAGGAA AGGGCGTCAC CGGGGAGAAA ATCTCACTCA GCCTATCACA
    GATAACAGTC CTATGAAGCG CTCGGCTTCA TCTTTGGGAC ACGGGCGTGC AGGACGCTCC
    ATGCGAGGTG AGGATTATGC CATGGAGAGG GTTATCCCAG AAGAGGGTCA CAGGCATGGG
    CATCGCCACC GAGACCGTAG TCACCGGGCG TCAGAACGCT CCCTGAGCCG ATACACTGAT
    GCTGACACAG GTCTTGGAAC TGACCTCAGC ACAACCACAC AATCAGGAGA CCTGCCATCC
    AAGGAGAGAG ACCGGGGCCG AGCCAAAGAC CGCAAGCACC ATCATCATCA TCATCACCAC
    CACGGCTCTT TAGATAAGGA GCACTACGGT CATGAGAGAG ACAGAGGAGA GTACGGCCAC
    AGACAGTCCA GAGAGAGAGA CCGCCGCTGG TCCCGCTCAC CCAGTGAGGG CAGAGAGTGT
    CTGACTCACA GACAGGGCAG TAGTTCAGTA AGCGGCAGTC CAGTCCCGTC CACCTCCGGG
    ACCAGCACCC CCCGCCGTGG CCGCCGTCAG CTCCCCCAAA CCCCTGCTAC ACCCCGGCCA
    CATGTCACCT ACTCACCTGT GGTCCGTAAG CCCATCAGCA GTACCCCCCC TCCTGGCCAG
    CAGAGCCGAC TCCCTACCCC GACCTCCCGG CGCTTCAGTC CGACTGGCCC CGAGCCCCCG
    CTTCCCCCTC ATCACCACCC GTCACCACCC CACCACGGCT CTCCCCGCTC CGGCCGACAT
    GCACACTGGG GCCCCGAGCC TGCAGAGAGC GTGGAGGGTG ACGGCTTCTA TGACGACCAA
    GACTATGAGT TCAATCACCA CGAGCCTCCA TCCTACGAGC AAAGCCCTGT GCAGGGCAAC
    CCTCACCCCC ATTCCCCTAG GACTTCCCGC CACAACACCC CTCCTCAGGG CCAAGCGCAC
    CCACGGCGCA TGCCAAATGG CTACCGTTCG TCTTCGCCCT CTCCACACCA CCACGCGCCC
    GCTCACCCTG GAACTCATAA ACCTCCTCAT CCCAGAGGGC CCAGAAAGGG CCTCCATGAA
    CCCTACAGTG AGACTGACGA GGATGACTGG TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141785
    Clone ID Related Accession (Same CDS sequence) XM_021474379.1
    Accession Version XM_021474379.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7113bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG
    GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT
    GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC
    CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC
    ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT
    GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT
    GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT
    GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT
    CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA
    AGGCAGTCTC AGACAGTAGA GATGAGAGAG ATGGGGCGGG ACGGGTACTC GGACACAGAG
    CAATATCTGC CGATGGAAGG CCATGGCAGG GCTGCTTCCA TGCCTCGACT CCCAGCAGAC
    AACCAACAGC CACGGAGGAA AGGGCGTCAC CGGGGAGAAA ATCTCACTCC TATCACAGAT
    AACAGTCCTA TGAAGCGCTC GGCTTCATCT TTGGGACACG GGCGTGCAGG ACGCTCCATG
    CGAGGTGAGG ATTATGCCAT GGAGAGGGTT ATCCCAGAAG AGGGTCACAG GCATGGGCAT
    CGCCACCGAG ACCGTAGTCA CCGGGCGTCA GAACGCTCCC TGAGCCGATA CACTGATGCT
    GACACAGGTC TTGGAACTGA CCTCAGCACA ACCACACAAT CAGGAGACCT GCCATCCAAG
    GAGAGAGACC GGGGCCGAGC CAAAGACCGC AAGCACCATC ATCATCATCA TCACCACCAC
    GGCTCTTTAG ATAAGGAGCA CTACGGTCAT GAGAGAGACA GAGGAGAGTA CGGCCACAGA
    CAGTCCAGAG AGAGAGACCG CCGCTGGTCC CGCTCACCCA GTGAGGGCAG AGAGTGTCTG
    ACTCACAGAC AGGGCAGTAG TTCAGTAAGC GGCAGTCCAG TCCCGTCCAC CTCCGGGACC
    AGCACCCCCC GCCGTGGCCG CCGTCAGCTC CCCCAAACCC CTGCTACACC CCGGCCACAT
    GTCACCTACT CACCTGTGGT CCGTAAGCCC ATCAGCAGTA CCCCCCCTCC TGGCCAGCAG
    AGCCGACTCC CTACCCCGAC CTCCCGGCGC TTCAGTCCGA CTGGCCCCGA GCCCCCGCTT
    CCCCCTCATC ACCACCCGTC ACCACCCCAC CACGGCTCTC CCCGCTCCGG CCGACATGCA
    CACTGGGGCC CCGAGCCTGC AGAGAGCGTG GAGGGTGACG GCTTCTATGA CGACCAAGAC
    TATGAGTTCA ATCACCACGA GCCTCCATCC TACGAGCAAA GCCCTGTGCA GGGCAACCCT
    CACCCCCATT CCCCTAGGAC TTCCCGCCAC AACACCCCTC CTCAGGGCCA AGCGCACCCA
    CGGCGCATGC CAAATGGCTA CCGTTCGTCT TCGCCCTCTC CACACCACCA CGCGCCCGCT
    CACCCTGGAA CTCATAAACC TCCTCATCCC AGAGGGCCCA GAAAGGGCCT CCATGAACCC
    TACAGTGAGA CTGACGAGGA TGACTGGTGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330054.1
    CDS1507..8619
    Translation

    Target ORF information:

    RefSeq Version XM_021474379.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474379.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG
    GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT
    GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC
    CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC
    ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT
    GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT
    GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT
    GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT
    CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA
    AGGCAGTCTC AGACAGTAGA GATGAGAGAG ATGGGGCGGG ACGGGTACTC GGACACAGAG
    CAATATCTGC CGATGGAAGG CCATGGCAGG GCTGCTTCCA TGCCTCGACT CCCAGCAGAC
    AACCAACAGC CACGGAGGAA AGGGCGTCAC CGGGGAGAAA ATCTCACTCC TATCACAGAT
    AACAGTCCTA TGAAGCGCTC GGCTTCATCT TTGGGACACG GGCGTGCAGG ACGCTCCATG
    CGAGGTGAGG ATTATGCCAT GGAGAGGGTT ATCCCAGAAG AGGGTCACAG GCATGGGCAT
    CGCCACCGAG ACCGTAGTCA CCGGGCGTCA GAACGCTCCC TGAGCCGATA CACTGATGCT
    GACACAGGTC TTGGAACTGA CCTCAGCACA ACCACACAAT CAGGAGACCT GCCATCCAAG
    GAGAGAGACC GGGGCCGAGC CAAAGACCGC AAGCACCATC ATCATCATCA TCACCACCAC
    GGCTCTTTAG ATAAGGAGCA CTACGGTCAT GAGAGAGACA GAGGAGAGTA CGGCCACAGA
    CAGTCCAGAG AGAGAGACCG CCGCTGGTCC CGCTCACCCA GTGAGGGCAG AGAGTGTCTG
    ACTCACAGAC AGGGCAGTAG TTCAGTAAGC GGCAGTCCAG TCCCGTCCAC CTCCGGGACC
    AGCACCCCCC GCCGTGGCCG CCGTCAGCTC CCCCAAACCC CTGCTACACC CCGGCCACAT
    GTCACCTACT CACCTGTGGT CCGTAAGCCC ATCAGCAGTA CCCCCCCTCC TGGCCAGCAG
    AGCCGACTCC CTACCCCGAC CTCCCGGCGC TTCAGTCCGA CTGGCCCCGA GCCCCCGCTT
    CCCCCTCATC ACCACCCGTC ACCACCCCAC CACGGCTCTC CCCGCTCCGG CCGACATGCA
    CACTGGGGCC CCGAGCCTGC AGAGAGCGTG GAGGGTGACG GCTTCTATGA CGACCAAGAC
    TATGAGTTCA ATCACCACGA GCCTCCATCC TACGAGCAAA GCCCTGTGCA GGGCAACCCT
    CACCCCCATT CCCCTAGGAC TTCCCGCCAC AACACCCCTC CTCAGGGCCA AGCGCACCCA
    CGGCGCATGC CAAATGGCTA CCGTTCGTCT TCGCCCTCTC CACACCACCA CGCGCCCGCT
    CACCCTGGAA CTCATAAACC TCCTCATCCC AGAGGGCCCA GAAAGGGCCT CCATGAACCC
    TACAGTGAGA CTGACGAGGA TGACTGGTGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141786
    Clone ID Related Accession (Same CDS sequence) XM_021474381.1
    Accession Version XM_021474381.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7113bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA ACAACTTCAT AAATCTGAGT
    TTTCTAAGGC TCTTCAGAGC TGCTCGTCTC ATTAAGTTGC TCAGGCAGGG TGAAACCATA
    CGCATCCTGC TCTGGACTTT TGTCCAGTCT TTCAAGGCTC TGCCGTATGT GTGCCTACTC
    ATCGCCATGC TGTTCTTCAT CTACGCCATC ATTGGCATGC AGCTGTTTGG GAACATTAAG
    ATCGAGGAGA ACAGTGACAG TGCCATTACC CAGCACAACA ACTTCAGGAC TTTTTTCCAG
    GCCCTAATGC TGCTCTTTAG GAGTGCTACA GGTGAGGCAT GGCATGACAT CATGCTGTCA
    TGTCTGGGAA AGAAGCCGTG TGATATTCTC TCTGATAACC CGAAGCCAGA ATGCGGGAGC
    GAGTTTGCCT ACCTCTACTT TGTCTCCTTC ATCTTCCTCT GTTCATTTCT GATGCTGAAT
    CTGTTCGTGG CTGTGATCAT GGATAATTTT GAATACCTGA CGCGTGACTC CTCTATCCTG
    GGACCCCATC ACCTGGACGA GTATGTGAGG ATCTGGGCAG AATATGATCC TGCTGCTTGC
    GGGCGCATTC ATTATAAGGA TATGTACAGT TTATTGCGAG TTATCGACCC CCCTCTGGGC
    TTAGGCAAGA AATGTCCTCA TAGGGTGGCC TGCAAGAGAC TGTTGCGGAT GGATCTGCCG
    GTTGCCGATG ACAACACAGT GCACTTTAAT TCTACCCTTA TGGCCTTAAT CCGAACCGCT
    CTGGACATCA AGATCGCCAA GGGTGGGGAA GGAGGTGTAG ATAAGCACCA GATGGATGCT
    GAATTAAGGA AGGAGATGAT GGCGATATGG CCTAATCTCT CTCAGAAGAA CCTGGACCTG
    CTGGTGACTC CTCACAAGTC AGCGACAGAC CTGACAGTAG GAAAGATCTA CGCTGCCATG
    ATGATCATGG AGTATTATCG GCAAAGTAAA GCCAAACGGA CACAAGCTCT CCATGATGAG
    CAGAATCGAA CGCCATTAAT GTTTCAGCGT CTGGAGCCGC CATCTCCTAG CCAGGATGTG
    GGACCAGGAC TCACTGGCCT TCCTGACACA CAAATGCACC CTGCAAATCA TCTGCCTGTT
    GATGAAAGGA TCCCAGAGAG CCAATCATGG GTGACAGCGC GAGCACAGGA GATTTCTCAA
    AAAGCAGGCA ACTGGAGTCC AGAGGGCCAA CATCCAGATG ACACCACAGA CAACAGAAGG
    CAGTCTCAGA CAGTAGAGAT GAGAGAGATG GGGCGGGACG GGTACTCGGA CACAGAGCAA
    TATCTGCCGA TGGAAGGCCA TGGCAGGGCT GCTTCCATGC CTCGACTCCC AGCAGACAAC
    CAACAGCCAC GGAGGAAAGG GCGTCACCGG GGAGAAAATC TCACTCAGCC TATCACAGAT
    AACAGTCCTA TGAAGCGCTC GGCTTCATCT TTGGGACACG GGCGTGCAGG ACGCTCCATG
    CGAGGTGAGG ATTATGCCAT GGAGAGGGTT ATCCCAGAAG AGGGTCACAG GCATGGGCAT
    CGCCACCGAG ACCGTAGTCA CCGGGCGTCA GAACGCTCCC TGAGCCGATA CACTGATGCT
    GACACAGGTC TTGGAACTGA CCTCAGCACA ACCACACAAT CAGGAGACCT GCCATCCAAG
    GAGAGAGACC GGGGCCGAGC CAAAGACCGC AAGCACCATC ATCATCATCA TCACCACCAC
    GGCTCTTTAG ATAAGGAGCA CTACGGTCAT GAGAGAGACA GAGGAGAGTA CGGCCACAGA
    CAGTCCAGAG AGAGAGACCG CCGCTGGTCC CGCTCACCCA GTGAGGGCAG AGAGTGTCTG
    ACTCACAGAC AGGGCAGTAG TTCAGTAAGC GGCAGTCCAG TCCCGTCCAC CTCCGGGACC
    AGCACCCCCC GCCGTGGCCG CCGTCAGCTC CCCCAAACCC CTGCTACACC CCGGCCACAT
    GTCACCTACT CACCTGTGGT CCGTAAGCCC ATCAGCAGTA CCCCCCCTCC TGGCCAGCAG
    AGCCGACTCC CTACCCCGAC CTCCCGGCGC TTCAGTCCGA CTGGCCCCGA GCCCCCGCTT
    CCCCCTCATC ACCACCCGTC ACCACCCCAC CACGGCTCTC CCCGCTCCGG CCGACATGCA
    CACTGGGGCC CCGAGCCTGC AGAGAGCGTG GAGGGTGACG GCTTCTATGA CGACCAAGAC
    TATGAGTTCA ATCACCACGA GCCTCCATCC TACGAGCAAA GCCCTGTGCA GGGCAACCCT
    CACCCCCATT CCCCTAGGAC TTCCCGCCAC AACACCCCTC CTCAGGGCCA AGCGCACCCA
    CGGCGCATGC CAAATGGCTA CCGTTCGTCT TCGCCCTCTC CACACCACCA CGCGCCCGCT
    CACCCTGGAA CTCATAAACC TCCTCATCCC AGAGGGCCCA GAAAGGGCCT CCATGAACCC
    TACAGTGAGA CTGACGAGGA TGACTGGTGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330056.1
    CDS1507..8619
    Translation

    Target ORF information:

    RefSeq Version XM_021474381.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474381.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA ACAACTTCAT AAATCTGAGT
    TTTCTAAGGC TCTTCAGAGC TGCTCGTCTC ATTAAGTTGC TCAGGCAGGG TGAAACCATA
    CGCATCCTGC TCTGGACTTT TGTCCAGTCT TTCAAGGCTC TGCCGTATGT GTGCCTACTC
    ATCGCCATGC TGTTCTTCAT CTACGCCATC ATTGGCATGC AGCTGTTTGG GAACATTAAG
    ATCGAGGAGA ACAGTGACAG TGCCATTACC CAGCACAACA ACTTCAGGAC TTTTTTCCAG
    GCCCTAATGC TGCTCTTTAG GAGTGCTACA GGTGAGGCAT GGCATGACAT CATGCTGTCA
    TGTCTGGGAA AGAAGCCGTG TGATATTCTC TCTGATAACC CGAAGCCAGA ATGCGGGAGC
    GAGTTTGCCT ACCTCTACTT TGTCTCCTTC ATCTTCCTCT GTTCATTTCT GATGCTGAAT
    CTGTTCGTGG CTGTGATCAT GGATAATTTT GAATACCTGA CGCGTGACTC CTCTATCCTG
    GGACCCCATC ACCTGGACGA GTATGTGAGG ATCTGGGCAG AATATGATCC TGCTGCTTGC
    GGGCGCATTC ATTATAAGGA TATGTACAGT TTATTGCGAG TTATCGACCC CCCTCTGGGC
    TTAGGCAAGA AATGTCCTCA TAGGGTGGCC TGCAAGAGAC TGTTGCGGAT GGATCTGCCG
    GTTGCCGATG ACAACACAGT GCACTTTAAT TCTACCCTTA TGGCCTTAAT CCGAACCGCT
    CTGGACATCA AGATCGCCAA GGGTGGGGAA GGAGGTGTAG ATAAGCACCA GATGGATGCT
    GAATTAAGGA AGGAGATGAT GGCGATATGG CCTAATCTCT CTCAGAAGAA CCTGGACCTG
    CTGGTGACTC CTCACAAGTC AGCGACAGAC CTGACAGTAG GAAAGATCTA CGCTGCCATG
    ATGATCATGG AGTATTATCG GCAAAGTAAA GCCAAACGGA CACAAGCTCT CCATGATGAG
    CAGAATCGAA CGCCATTAAT GTTTCAGCGT CTGGAGCCGC CATCTCCTAG CCAGGATGTG
    GGACCAGGAC TCACTGGCCT TCCTGACACA CAAATGCACC CTGCAAATCA TCTGCCTGTT
    GATGAAAGGA TCCCAGAGAG CCAATCATGG GTGACAGCGC GAGCACAGGA GATTTCTCAA
    AAAGCAGGCA ACTGGAGTCC AGAGGGCCAA CATCCAGATG ACACCACAGA CAACAGAAGG
    CAGTCTCAGA CAGTAGAGAT GAGAGAGATG GGGCGGGACG GGTACTCGGA CACAGAGCAA
    TATCTGCCGA TGGAAGGCCA TGGCAGGGCT GCTTCCATGC CTCGACTCCC AGCAGACAAC
    CAACAGCCAC GGAGGAAAGG GCGTCACCGG GGAGAAAATC TCACTCAGCC TATCACAGAT
    AACAGTCCTA TGAAGCGCTC GGCTTCATCT TTGGGACACG GGCGTGCAGG ACGCTCCATG
    CGAGGTGAGG ATTATGCCAT GGAGAGGGTT ATCCCAGAAG AGGGTCACAG GCATGGGCAT
    CGCCACCGAG ACCGTAGTCA CCGGGCGTCA GAACGCTCCC TGAGCCGATA CACTGATGCT
    GACACAGGTC TTGGAACTGA CCTCAGCACA ACCACACAAT CAGGAGACCT GCCATCCAAG
    GAGAGAGACC GGGGCCGAGC CAAAGACCGC AAGCACCATC ATCATCATCA TCACCACCAC
    GGCTCTTTAG ATAAGGAGCA CTACGGTCAT GAGAGAGACA GAGGAGAGTA CGGCCACAGA
    CAGTCCAGAG AGAGAGACCG CCGCTGGTCC CGCTCACCCA GTGAGGGCAG AGAGTGTCTG
    ACTCACAGAC AGGGCAGTAG TTCAGTAAGC GGCAGTCCAG TCCCGTCCAC CTCCGGGACC
    AGCACCCCCC GCCGTGGCCG CCGTCAGCTC CCCCAAACCC CTGCTACACC CCGGCCACAT
    GTCACCTACT CACCTGTGGT CCGTAAGCCC ATCAGCAGTA CCCCCCCTCC TGGCCAGCAG
    AGCCGACTCC CTACCCCGAC CTCCCGGCGC TTCAGTCCGA CTGGCCCCGA GCCCCCGCTT
    CCCCCTCATC ACCACCCGTC ACCACCCCAC CACGGCTCTC CCCGCTCCGG CCGACATGCA
    CACTGGGGCC CCGAGCCTGC AGAGAGCGTG GAGGGTGACG GCTTCTATGA CGACCAAGAC
    TATGAGTTCA ATCACCACGA GCCTCCATCC TACGAGCAAA GCCCTGTGCA GGGCAACCCT
    CACCCCCATT CCCCTAGGAC TTCCCGCCAC AACACCCCTC CTCAGGGCCA AGCGCACCCA
    CGGCGCATGC CAAATGGCTA CCGTTCGTCT TCGCCCTCTC CACACCACCA CGCGCCCGCT
    CACCCTGGAA CTCATAAACC TCCTCATCCC AGAGGGCCCA GAAAGGGCCT CCATGAACCC
    TACAGTGAGA CTGACGAGGA TGACTGGTGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141787
    Clone ID Related Accession (Same CDS sequence) XM_021474386.1
    Accession Version XM_021474386.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7113bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG
    GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT
    GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC
    CTGCTGGTGA CTCCTCACAA GTCGACAGAC CTGACAGTAG GAAAGATCTA CGCTGCCATG
    ATGATCATGG AGTATTATCG GCAAAGTAAA GCCAAACGGA CACAAGCTCT CCATGATGAG
    CAGAATCGAA CGCCATTAAT GTTTCAGCGT CTGGAGCCGC CATCTCCTAG CCAGGATGTG
    GGACCAGGAC TCACTGGCCT TCCTGACACA CAAATGCACC CTGCAAATCA TCTGCCTGTT
    GATGAAAGGA TCCCAGAGAG CCAATCATGG GTGACAGCGC GAGCACAGGA GATTTCTCAA
    AAAGCAGGCA ACTGGAGTCC AGAGGGCCAA CATCCAGATG ACACCACAGA CAACAGAAGG
    CAGTCTCAGA CAGTAGAGAT GAGAGAGATG GGGCGGGACG GGTACTCGGA CACAGAGCAA
    TATCTGCCGA TGGAAGGCCA TGGCAGGGCT GCTTCCATGC CTCGACTCCC AGCAGACAAC
    CAACAGCCAC GGAGGAAAGG GCGTCACCGG GGAGAAAATC TCACTCAGCC TATCACAGAT
    AACAGTCCTA TGAAGCGCTC GGCTTCATCT TTGGGACACG GGCGTGCAGG ACGCTCCATG
    CGAGGTGAGG ATTATGCCAT GGAGAGGGTT ATCCCAGAAG AGGGTCACAG GCATGGGCAT
    CGCCACCGAG ACCGTAGTCA CCGGGCGTCA GAACGCTCCC TGAGCCGATA CACTGATGCT
    GACACAGGTC TTGGAACTGA CCTCAGCACA ACCACACAAT CAGGAGACCT GCCATCCAAG
    GAGAGAGACC GGGGCCGAGC CAAAGACCGC AAGCACCATC ATCATCATCA TCACCACCAC
    GGCTCTTTAG ATAAGGAGCA CTACGGTCAT GAGAGAGACA GAGGAGAGTA CGGCCACAGA
    CAGTCCAGAG AGAGAGACCG CCGCTGGTCC CGCTCACCCA GTGAGGGCAG AGAGTGTCTG
    ACTCACAGAC AGGGCAGTAG TTCAGTAAGC GGCAGTCCAG TCCCGTCCAC CTCCGGGACC
    AGCACCCCCC GCCGTGGCCG CCGTCAGCTC CCCCAAACCC CTGCTACACC CCGGCCACAT
    GTCACCTACT CACCTGTGGT CCGTAAGCCC ATCAGCAGTA CCCCCCCTCC TGGCCAGCAG
    AGCCGACTCC CTACCCCGAC CTCCCGGCGC TTCAGTCCGA CTGGCCCCGA GCCCCCGCTT
    CCCCCTCATC ACCACCCGTC ACCACCCCAC CACGGCTCTC CCCGCTCCGG CCGACATGCA
    CACTGGGGCC CCGAGCCTGC AGAGAGCGTG GAGGGTGACG GCTTCTATGA CGACCAAGAC
    TATGAGTTCA ATCACCACGA GCCTCCATCC TACGAGCAAA GCCCTGTGCA GGGCAACCCT
    CACCCCCATT CCCCTAGGAC TTCCCGCCAC AACACCCCTC CTCAGGGCCA AGCGCACCCA
    CGGCGCATGC CAAATGGCTA CCGTTCGTCT TCGCCCTCTC CACACCACCA CGCGCCCGCT
    CACCCTGGAA CTCATAAACC TCCTCATCCC AGAGGGCCCA GAAAGGGCCT CCATGAACCC
    TACAGTGAGA CTGACGAGGA TGACTGGTGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330061.1
    CDS1508..8620
    Translation

    Target ORF information:

    RefSeq Version XM_021474386.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474386.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG
    GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT
    GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC
    CTGCTGGTGA CTCCTCACAA GTCGACAGAC CTGACAGTAG GAAAGATCTA CGCTGCCATG
    ATGATCATGG AGTATTATCG GCAAAGTAAA GCCAAACGGA CACAAGCTCT CCATGATGAG
    CAGAATCGAA CGCCATTAAT GTTTCAGCGT CTGGAGCCGC CATCTCCTAG CCAGGATGTG
    GGACCAGGAC TCACTGGCCT TCCTGACACA CAAATGCACC CTGCAAATCA TCTGCCTGTT
    GATGAAAGGA TCCCAGAGAG CCAATCATGG GTGACAGCGC GAGCACAGGA GATTTCTCAA
    AAAGCAGGCA ACTGGAGTCC AGAGGGCCAA CATCCAGATG ACACCACAGA CAACAGAAGG
    CAGTCTCAGA CAGTAGAGAT GAGAGAGATG GGGCGGGACG GGTACTCGGA CACAGAGCAA
    TATCTGCCGA TGGAAGGCCA TGGCAGGGCT GCTTCCATGC CTCGACTCCC AGCAGACAAC
    CAACAGCCAC GGAGGAAAGG GCGTCACCGG GGAGAAAATC TCACTCAGCC TATCACAGAT
    AACAGTCCTA TGAAGCGCTC GGCTTCATCT TTGGGACACG GGCGTGCAGG ACGCTCCATG
    CGAGGTGAGG ATTATGCCAT GGAGAGGGTT ATCCCAGAAG AGGGTCACAG GCATGGGCAT
    CGCCACCGAG ACCGTAGTCA CCGGGCGTCA GAACGCTCCC TGAGCCGATA CACTGATGCT
    GACACAGGTC TTGGAACTGA CCTCAGCACA ACCACACAAT CAGGAGACCT GCCATCCAAG
    GAGAGAGACC GGGGCCGAGC CAAAGACCGC AAGCACCATC ATCATCATCA TCACCACCAC
    GGCTCTTTAG ATAAGGAGCA CTACGGTCAT GAGAGAGACA GAGGAGAGTA CGGCCACAGA
    CAGTCCAGAG AGAGAGACCG CCGCTGGTCC CGCTCACCCA GTGAGGGCAG AGAGTGTCTG
    ACTCACAGAC AGGGCAGTAG TTCAGTAAGC GGCAGTCCAG TCCCGTCCAC CTCCGGGACC
    AGCACCCCCC GCCGTGGCCG CCGTCAGCTC CCCCAAACCC CTGCTACACC CCGGCCACAT
    GTCACCTACT CACCTGTGGT CCGTAAGCCC ATCAGCAGTA CCCCCCCTCC TGGCCAGCAG
    AGCCGACTCC CTACCCCGAC CTCCCGGCGC TTCAGTCCGA CTGGCCCCGA GCCCCCGCTT
    CCCCCTCATC ACCACCCGTC ACCACCCCAC CACGGCTCTC CCCGCTCCGG CCGACATGCA
    CACTGGGGCC CCGAGCCTGC AGAGAGCGTG GAGGGTGACG GCTTCTATGA CGACCAAGAC
    TATGAGTTCA ATCACCACGA GCCTCCATCC TACGAGCAAA GCCCTGTGCA GGGCAACCCT
    CACCCCCATT CCCCTAGGAC TTCCCGCCAC AACACCCCTC CTCAGGGCCA AGCGCACCCA
    CGGCGCATGC CAAATGGCTA CCGTTCGTCT TCGCCCTCTC CACACCACCA CGCGCCCGCT
    CACCCTGGAA CTCATAAACC TCCTCATCCC AGAGGGCCCA GAAAGGGCCT CCATGAACCC
    TACAGTGAGA CTGACGAGGA TGACTGGTGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141788
    Clone ID Related Accession (Same CDS sequence) XM_021474390.1
    Accession Version XM_021474390.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7107bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG
    GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGAGGT GTAGATAAGC ACCAGATGGA TGCTGAATTA
    AGGAAGGAGA TGATGGCGAT ATGGCCTAAT CTCTCTCAGA AGAACCTGGA CCTGCTGGTG
    ACTCCTCACA AGTCAGCGAC AGACCTGACA GTAGGAAAGA TCTACGCTGC CATGATGATC
    ATGGAGTATT ATCGGCAAAG TAAAGCCAAA CGGACACAAG CTCTCCATGA TGAGCAGAAT
    CGAACGCCAT TAATGTTTCA GCGTCTGGAG CCGCCATCTC CTAGCCAGGA TGTGGGACCA
    GGACTCACTG GCCTTCCTGA CACACAAATG CACCCTGCAA ATCATCTGCC TGTTGATGAA
    AGGATCCCAG AGAGCCAATC ATGGGTGACA GCGCGAGCAC AGGAGATTTC TCAAAAAGCA
    GGCAACTGGA GTCCAGAGGG CCAACATCCA GATGACACCA CAGACAACAG AAGGCAGTCT
    CAGACAGTAG AGATGAGAGA GATGGGGCGG GACGGGTACT CGGACACAGA GCAATATCTG
    CCGATGGAAG GCCATGGCAG GGCTGCTTCC ATGCCTCGAC TCCCAGCAGA CAACCAACAG
    CCACGGAGGA AAGGGCGTCA CCGGGGAGAA AATCTCACTC AGCCTATCAC AGATAACAGT
    CCTATGAAGC GCTCGGCTTC ATCTTTGGGA CACGGGCGTG CAGGACGCTC CATGCGAGGT
    GAGGATTATG CCATGGAGAG GGTTATCCCA GAAGAGGGTC ACAGGCATGG GCATCGCCAC
    CGAGACCGTA GTCACCGGGC GTCAGAACGC TCCCTGAGCC GATACACTGA TGCTGACACA
    GGTCTTGGAA CTGACCTCAG CACAACCACA CAATCAGGAG ACCTGCCATC CAAGGAGAGA
    GACCGGGGCC GAGCCAAAGA CCGCAAGCAC CATCATCATC ATCATCACCA CCACGGCTCT
    TTAGATAAGG AGCACTACGG TCATGAGAGA GACAGAGGAG AGTACGGCCA CAGACAGTCC
    AGAGAGAGAG ACCGCCGCTG GTCCCGCTCA CCCAGTGAGG GCAGAGAGTG TCTGACTCAC
    AGACAGGGCA GTAGTTCAGT AAGCGGCAGT CCAGTCCCGT CCACCTCCGG GACCAGCACC
    CCCCGCCGTG GCCGCCGTCA GCTCCCCCAA ACCCCTGCTA CACCCCGGCC ACATGTCACC
    TACTCACCTG TGGTCCGTAA GCCCATCAGC AGTACCCCCC CTCCTGGCCA GCAGAGCCGA
    CTCCCTACCC CGACCTCCCG GCGCTTCAGT CCGACTGGCC CCGAGCCCCC GCTTCCCCCT
    CATCACCACC CGTCACCACC CCACCACGGC TCTCCCCGCT CCGGCCGACA TGCACACTGG
    GGCCCCGAGC CTGCAGAGAG CGTGGAGGGT GACGGCTTCT ATGACGACCA AGACTATGAG
    TTCAATCACC ACGAGCCTCC ATCCTACGAG CAAAGCCCTG TGCAGGGCAA CCCTCACCCC
    CATTCCCCTA GGACTTCCCG CCACAACACC CCTCCTCAGG GCCAAGCGCA CCCACGGCGC
    ATGCCAAATG GCTACCGTTC GTCTTCGCCC TCTCCACACC ACCACGCGCC CGCTCACCCT
    GGAACTCATA AACCTCCTCA TCCCAGAGGG CCCAGAAAGG GCCTCCATGA ACCCTACAGT
    GAGACTGACG AGGATGACTG GTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330065.1
    CDS1509..8615
    Translation

    Target ORF information:

    RefSeq Version XM_021474390.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474390.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG
    GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGAGGT GTAGATAAGC ACCAGATGGA TGCTGAATTA
    AGGAAGGAGA TGATGGCGAT ATGGCCTAAT CTCTCTCAGA AGAACCTGGA CCTGCTGGTG
    ACTCCTCACA AGTCAGCGAC AGACCTGACA GTAGGAAAGA TCTACGCTGC CATGATGATC
    ATGGAGTATT ATCGGCAAAG TAAAGCCAAA CGGACACAAG CTCTCCATGA TGAGCAGAAT
    CGAACGCCAT TAATGTTTCA GCGTCTGGAG CCGCCATCTC CTAGCCAGGA TGTGGGACCA
    GGACTCACTG GCCTTCCTGA CACACAAATG CACCCTGCAA ATCATCTGCC TGTTGATGAA
    AGGATCCCAG AGAGCCAATC ATGGGTGACA GCGCGAGCAC AGGAGATTTC TCAAAAAGCA
    GGCAACTGGA GTCCAGAGGG CCAACATCCA GATGACACCA CAGACAACAG AAGGCAGTCT
    CAGACAGTAG AGATGAGAGA GATGGGGCGG GACGGGTACT CGGACACAGA GCAATATCTG
    CCGATGGAAG GCCATGGCAG GGCTGCTTCC ATGCCTCGAC TCCCAGCAGA CAACCAACAG
    CCACGGAGGA AAGGGCGTCA CCGGGGAGAA AATCTCACTC AGCCTATCAC AGATAACAGT
    CCTATGAAGC GCTCGGCTTC ATCTTTGGGA CACGGGCGTG CAGGACGCTC CATGCGAGGT
    GAGGATTATG CCATGGAGAG GGTTATCCCA GAAGAGGGTC ACAGGCATGG GCATCGCCAC
    CGAGACCGTA GTCACCGGGC GTCAGAACGC TCCCTGAGCC GATACACTGA TGCTGACACA
    GGTCTTGGAA CTGACCTCAG CACAACCACA CAATCAGGAG ACCTGCCATC CAAGGAGAGA
    GACCGGGGCC GAGCCAAAGA CCGCAAGCAC CATCATCATC ATCATCACCA CCACGGCTCT
    TTAGATAAGG AGCACTACGG TCATGAGAGA GACAGAGGAG AGTACGGCCA CAGACAGTCC
    AGAGAGAGAG ACCGCCGCTG GTCCCGCTCA CCCAGTGAGG GCAGAGAGTG TCTGACTCAC
    AGACAGGGCA GTAGTTCAGT AAGCGGCAGT CCAGTCCCGT CCACCTCCGG GACCAGCACC
    CCCCGCCGTG GCCGCCGTCA GCTCCCCCAA ACCCCTGCTA CACCCCGGCC ACATGTCACC
    TACTCACCTG TGGTCCGTAA GCCCATCAGC AGTACCCCCC CTCCTGGCCA GCAGAGCCGA
    CTCCCTACCC CGACCTCCCG GCGCTTCAGT CCGACTGGCC CCGAGCCCCC GCTTCCCCCT
    CATCACCACC CGTCACCACC CCACCACGGC TCTCCCCGCT CCGGCCGACA TGCACACTGG
    GGCCCCGAGC CTGCAGAGAG CGTGGAGGGT GACGGCTTCT ATGACGACCA AGACTATGAG
    TTCAATCACC ACGAGCCTCC ATCCTACGAG CAAAGCCCTG TGCAGGGCAA CCCTCACCCC
    CATTCCCCTA GGACTTCCCG CCACAACACC CCTCCTCAGG GCCAAGCGCA CCCACGGCGC
    ATGCCAAATG GCTACCGTTC GTCTTCGCCC TCTCCACACC ACCACGCGCC CGCTCACCCT
    GGAACTCATA AACCTCCTCA TCCCAGAGGG CCCAGAAAGG GCCTCCATGA ACCCTACAGT
    GAGACTGACG AGGATGACTG GTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141789
    Clone ID Related Accession (Same CDS sequence) XM_021474393.1
    Accession Version XM_021474393.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7107bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGTGGCAGAA TCAGTTACAG GGATATGTAT GAGATGCTCA GGCACATGTC TCCTCCGTTA
    GGCCTGGGGA AGAAGTGCCC TGCACGGGTT GCCTACAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGAGGT GTAGATAAGC ACCAGATGGA TGCTGAATTA
    AGGAAGGAGA TGATGGCGAT ATGGCCTAAT CTCTCTCAGA AGAACCTGGA CCTGCTGGTG
    ACTCCTCACA AGTCAGCGAC AGACCTGACA GTAGGAAAGA TCTACGCTGC CATGATGATC
    ATGGAGTATT ATCGGCAAAG TAAAGCCAAA CGGACACAAG CTCTCCATGA TGAGCAGAAT
    CGAACGCCAT TAATGTTTCA GCGTCTGGAG CCGCCATCTC CTAGCCAGGA TGTGGGACCA
    GGACTCACTG GCCTTCCTGA CACACAAATG CACCCTGCAA ATCATCTGCC TGTTGATGAA
    AGGATCCCAG AGAGCCAATC ATGGGTGACA GCGCGAGCAC AGGAGATTTC TCAAAAAGCA
    GGCAACTGGA GTCCAGAGGG CCAACATCCA GATGACACCA CAGACAACAG AAGGCAGTCT
    CAGACAGTAG AGATGAGAGA GATGGGGCGG GACGGGTACT CGGACACAGA GCAATATCTG
    CCGATGGAAG GCCATGGCAG GGCTGCTTCC ATGCCTCGAC TCCCAGCAGA CAACCAACAG
    CCACGGAGGA AAGGGCGTCA CCGGGGAGAA AATCTCACTC AGCCTATCAC AGATAACAGT
    CCTATGAAGC GCTCGGCTTC ATCTTTGGGA CACGGGCGTG CAGGACGCTC CATGCGAGGT
    GAGGATTATG CCATGGAGAG GGTTATCCCA GAAGAGGGTC ACAGGCATGG GCATCGCCAC
    CGAGACCGTA GTCACCGGGC GTCAGAACGC TCCCTGAGCC GATACACTGA TGCTGACACA
    GGTCTTGGAA CTGACCTCAG CACAACCACA CAATCAGGAG ACCTGCCATC CAAGGAGAGA
    GACCGGGGCC GAGCCAAAGA CCGCAAGCAC CATCATCATC ATCATCACCA CCACGGCTCT
    TTAGATAAGG AGCACTACGG TCATGAGAGA GACAGAGGAG AGTACGGCCA CAGACAGTCC
    AGAGAGAGAG ACCGCCGCTG GTCCCGCTCA CCCAGTGAGG GCAGAGAGTG TCTGACTCAC
    AGACAGGGCA GTAGTTCAGT AAGCGGCAGT CCAGTCCCGT CCACCTCCGG GACCAGCACC
    CCCCGCCGTG GCCGCCGTCA GCTCCCCCAA ACCCCTGCTA CACCCCGGCC ACATGTCACC
    TACTCACCTG TGGTCCGTAA GCCCATCAGC AGTACCCCCC CTCCTGGCCA GCAGAGCCGA
    CTCCCTACCC CGACCTCCCG GCGCTTCAGT CCGACTGGCC CCGAGCCCCC GCTTCCCCCT
    CATCACCACC CGTCACCACC CCACCACGGC TCTCCCCGCT CCGGCCGACA TGCACACTGG
    GGCCCCGAGC CTGCAGAGAG CGTGGAGGGT GACGGCTTCT ATGACGACCA AGACTATGAG
    TTCAATCACC ACGAGCCTCC ATCCTACGAG CAAAGCCCTG TGCAGGGCAA CCCTCACCCC
    CATTCCCCTA GGACTTCCCG CCACAACACC CCTCCTCAGG GCCAAGCGCA CCCACGGCGC
    ATGCCAAATG GCTACCGTTC GTCTTCGCCC TCTCCACACC ACCACGCGCC CGCTCACCCT
    GGAACTCATA AACCTCCTCA TCCCAGAGGG CCCAGAAAGG GCCTCCATGA ACCCTACAGT
    GAGACTGACG AGGATGACTG GTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330068.1
    CDS1509..8615
    Translation

    Target ORF information:

    RefSeq Version XM_021474393.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474393.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGTGGCAGAA TCAGTTACAG GGATATGTAT GAGATGCTCA GGCACATGTC TCCTCCGTTA
    GGCCTGGGGA AGAAGTGCCC TGCACGGGTT GCCTACAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGAGGT GTAGATAAGC ACCAGATGGA TGCTGAATTA
    AGGAAGGAGA TGATGGCGAT ATGGCCTAAT CTCTCTCAGA AGAACCTGGA CCTGCTGGTG
    ACTCCTCACA AGTCAGCGAC AGACCTGACA GTAGGAAAGA TCTACGCTGC CATGATGATC
    ATGGAGTATT ATCGGCAAAG TAAAGCCAAA CGGACACAAG CTCTCCATGA TGAGCAGAAT
    CGAACGCCAT TAATGTTTCA GCGTCTGGAG CCGCCATCTC CTAGCCAGGA TGTGGGACCA
    GGACTCACTG GCCTTCCTGA CACACAAATG CACCCTGCAA ATCATCTGCC TGTTGATGAA
    AGGATCCCAG AGAGCCAATC ATGGGTGACA GCGCGAGCAC AGGAGATTTC TCAAAAAGCA
    GGCAACTGGA GTCCAGAGGG CCAACATCCA GATGACACCA CAGACAACAG AAGGCAGTCT
    CAGACAGTAG AGATGAGAGA GATGGGGCGG GACGGGTACT CGGACACAGA GCAATATCTG
    CCGATGGAAG GCCATGGCAG GGCTGCTTCC ATGCCTCGAC TCCCAGCAGA CAACCAACAG
    CCACGGAGGA AAGGGCGTCA CCGGGGAGAA AATCTCACTC AGCCTATCAC AGATAACAGT
    CCTATGAAGC GCTCGGCTTC ATCTTTGGGA CACGGGCGTG CAGGACGCTC CATGCGAGGT
    GAGGATTATG CCATGGAGAG GGTTATCCCA GAAGAGGGTC ACAGGCATGG GCATCGCCAC
    CGAGACCGTA GTCACCGGGC GTCAGAACGC TCCCTGAGCC GATACACTGA TGCTGACACA
    GGTCTTGGAA CTGACCTCAG CACAACCACA CAATCAGGAG ACCTGCCATC CAAGGAGAGA
    GACCGGGGCC GAGCCAAAGA CCGCAAGCAC CATCATCATC ATCATCACCA CCACGGCTCT
    TTAGATAAGG AGCACTACGG TCATGAGAGA GACAGAGGAG AGTACGGCCA CAGACAGTCC
    AGAGAGAGAG ACCGCCGCTG GTCCCGCTCA CCCAGTGAGG GCAGAGAGTG TCTGACTCAC
    AGACAGGGCA GTAGTTCAGT AAGCGGCAGT CCAGTCCCGT CCACCTCCGG GACCAGCACC
    CCCCGCCGTG GCCGCCGTCA GCTCCCCCAA ACCCCTGCTA CACCCCGGCC ACATGTCACC
    TACTCACCTG TGGTCCGTAA GCCCATCAGC AGTACCCCCC CTCCTGGCCA GCAGAGCCGA
    CTCCCTACCC CGACCTCCCG GCGCTTCAGT CCGACTGGCC CCGAGCCCCC GCTTCCCCCT
    CATCACCACC CGTCACCACC CCACCACGGC TCTCCCCGCT CCGGCCGACA TGCACACTGG
    GGCCCCGAGC CTGCAGAGAG CGTGGAGGGT GACGGCTTCT ATGACGACCA AGACTATGAG
    TTCAATCACC ACGAGCCTCC ATCCTACGAG CAAAGCCCTG TGCAGGGCAA CCCTCACCCC
    CATTCCCCTA GGACTTCCCG CCACAACACC CCTCCTCAGG GCCAAGCGCA CCCACGGCGC
    ATGCCAAATG GCTACCGTTC GTCTTCGCCC TCTCCACACC ACCACGCGCC CGCTCACCCT
    GGAACTCATA AACCTCCTCA TCCCAGAGGG CCCAGAAAGG GCCTCCATGA ACCCTACAGT
    GAGACTGACG AGGATGACTG GTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141790
    Clone ID Related Accession (Same CDS sequence) XM_021474394.1
    Accession Version XM_021474394.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7104bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    121
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    301
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    481
    541
    601
    661
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    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
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    2641
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    3181
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    4141
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    4441
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    4681
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    5521
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    5881
    5941
    6001
    6061
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    6181
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    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACAGGAAGC AGCAAAGGGA AAGACATCAG TACTATCAAG
    TCTCTACGTG TGCTTCGAGT ACTGCGGCCG CTGAAAACCA TCAAACGTCT GCCAAAACTG
    AAGGCTGTCT TTGACTGTGT GGTGAACTCT CTGAAGAATG TGTTGAACAT CCTCATCGTC
    TACATGCTCT TCATGTTCAT CTTCGCTGTG GTGGCTGTGC AGCTCTTTAA GGGACGTTTC
    TTTTACTGCA CTGATGAATC CAAAGAGTTT GAGCGCGACT GCAGGGGCGA GTATCTGGTC
    TATGAAAGAG ATAACGAAGT GAGGTCACAG AAGCGGGAAT GGAAGAAATA CGATTTCCAC
    TACGACAACG TGCTCTGGGC CCTTCTCACC CTCTTCACCG TTTCTACGGG AGAGGGGTGG
    CCGCAAGTGC TAAAACATTC AGTAGATGCG ACCTACGAGA ATCAGGGCCC GAGCCCAGGT
    TACCGGATGG AAATGTCCAT CTTTTACGTG GTGTACTTCG TGGTCTTCCC CTTCTTCTTC
    GTCAACATCT TCGTGGCTCT TATCATCATT ACTTTCCAGG AGCAAGGAGA CAAAATGATG
    GAGGACTATA GCCTGGAAAA GAATGAGAGA GCCTGCATAG ACTTTGCCAT CAACGCTAAG
    CCGCTCACAC GGCACATGCC CCAGAACAAG CAGACCTTTC AGTACCGCAT GTGGGAGTTT
    GTGGTGTCTC CTCCATTTGA ATACACCATC ATGGCCTTGA TCGCGCTCAA CACTATTGTC
    CTTATGATGA AGTATGATGG AGCATCGCTT ACTTATGAAG ATGTGCTAAA ATACCTGAAC
    ATAGTGTTCA CCTCTCTATT CTCCATGGAG TGCATATTGA AGATCATAGC CTTCGGAGCA
    TTGAACTACT TCAAAGATGC CTGGAACATC TTCGACTGTG TGACTGTACT TGGCAGCATC
    ACTGATATTT TAGTCACAGA GCTGGGGATG AACAACTTCA TAAATCTGAG TTTTCTAAGG
    CTCTTCAGAG CTGCTCGTCT CATTAAGTTG CTCAGGCAGG GTGAAACCAT ACGCATCCTG
    CTCTGGACTT TTGTCCAGTC TTTCAAGGCT CTGCCGTATG TGTGCCTACT CATCGCCATG
    CTGTTCTTCA TCTACGCCAT CATTGGCATG CAGCTGTTTG GGAACATTAA GATCGAGGAG
    AACAGTGACA GTGCCATTAC CCAGCACAAC AACTTCAGGA CTTTTTTCCA GGCCCTAATG
    CTGCTCTTTA GGAGTGCTAC AGGTGAGGCA TGGCATGACA TCATGCTGTC ATGTCTGGGA
    AAGAAGCCGT GTGATATTCT CTCTGATAAC CCGAAGCCAG AATGCGGGAG CGAGTTTGCC
    TACCTCTACT TTGTCTCCTT CATCTTCCTC TGTTCATTTC TGATGCTGAA TCTGTTCGTG
    GCTGTGATCA TGGATAATTT TGAATACCTG ACGCGTGACT CCTCTATCCT GGGACCCCAT
    CACCTGGACG AGTATGTGAG GATCTGGGCA GAATATGATC CTGCTGCTTG CGGGCGCATT
    CATTATAAGG ATATGTACAG TTTATTGCGA GTTATCGACC CCCCTCTGGG CTTAGGCAAG
    AAATGTCCTC ATAGGGTGGC CTGCAAGAGA CTGTTGCGGA TGGATCTGCC GGTTGCCGAT
    GACAACACAG TGCACTTTAA TTCTACCCTT ATGGCCTTAA TCCGAACCGC TCTGGACATC
    AAGATCGCCA AGGGTGGGGA AGGAGGTGTA GATAAGCACC AGATGGATGC TGAATTAAGG
    AAGGAGATGA TGGCGATATG GCCTAATCTC TCTCAGAAGA ACCTGGACCT GCTGGTGACT
    CCTCACAAGT CAGCGACAGA CCTGACAGTA GGAAAGATCT ACGCTGCCAT GATGATCATG
    GAGTATTATC GGCAAAGTAA AGCCAAACGG ACACAAGCTC TCCATGATGA GCAGAATCGA
    ACGCCATTAA TGTTTCAGCG TCTGGAGCCG CCATCTCCTA GCCAGGATGT GGGACCAGGA
    CTCACTGGCC TTCCTGACAC ACAAATGCAC CCTGCAAATC ATCTGCCTGT TGATGAAAGG
    ATCCCAGAGA GCCAATCATG GGTGACAGCG CGAGCACAGG AGATTTCTCA AAAAGCAGGC
    AACTGGAGTC CAGAGGGCCA ACATCCAGAT GACACCACAG ACAACAGAAG GCAGTCTCAG
    ACAGTAGAGA TGAGAGAGAT GGGGCGGGAC GGGTACTCGG ACACAGAGCA ATATCTGCCG
    ATGGAAGGCC ATGGCAGGGC TGCTTCCATG CCTCGACTCC CAGCAGACAA CCAACAGCCA
    CGGAGGAAAG GGCGTCACCG GGGAGAAAAT CTCACTCAGC CTATCACAGA TAACAGTCCT
    ATGAAGCGCT CGGCTTCATC TTTGGGACAC GGGCGTGCAG GACGCTCCAT GCGAGGTGAG
    GATTATGCCA TGGAGAGGGT TATCCCAGAA GAGGGTCACA GGCATGGGCA TCGCCACCGA
    GACCGTAGTC ACCGGGCGTC AGAACGCTCC CTGAGCCGAT ACACTGATGC TGACACAGGT
    CTTGGAACTG ACCTCAGCAC AACCACACAA TCAGGAGACC TGCCATCCAA GGAGAGAGAC
    CGGGGCCGAG CCAAAGACCG CAAGCACCAT CATCATCATC ATCACCACCA CGGCTCTTTA
    GATAAGGAGC ACTACGGTCA TGAGAGAGAC AGAGGAGAGT ACGGCCACAG ACAGTCCAGA
    GAGAGAGACC GCCGCTGGTC CCGCTCACCC AGTGAGGGCA GAGAGTGTCT GACTCACAGA
    CAGGGCAGTA GTTCAGTAAG CGGCAGTCCA GTCCCGTCCA CCTCCGGGAC CAGCACCCCC
    CGCCGTGGCC GCCGTCAGCT CCCCCAAACC CCTGCTACAC CCCGGCCACA TGTCACCTAC
    TCACCTGTGG TCCGTAAGCC CATCAGCAGT ACCCCCCCTC CTGGCCAGCA GAGCCGACTC
    CCTACCCCGA CCTCCCGGCG CTTCAGTCCG ACTGGCCCCG AGCCCCCGCT TCCCCCTCAT
    CACCACCCGT CACCACCCCA CCACGGCTCT CCCCGCTCCG GCCGACATGC ACACTGGGGC
    CCCGAGCCTG CAGAGAGCGT GGAGGGTGAC GGCTTCTATG ACGACCAAGA CTATGAGTTC
    AATCACCACG AGCCTCCATC CTACGAGCAA AGCCCTGTGC AGGGCAACCC TCACCCCCAT
    TCCCCTAGGA CTTCCCGCCA CAACACCCCT CCTCAGGGCC AAGCGCACCC ACGGCGCATG
    CCAAATGGCT ACCGTTCGTC TTCGCCCTCT CCACACCACC ACGCGCCCGC TCACCCTGGA
    ACTCATAAAC CTCCTCATCC CAGAGGGCCC AGAAAGGGCC TCCATGAACC CTACAGTGAG
    ACTGACGAGG ATGACTGGTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330069.1
    CDS1507..8610
    Translation

    Target ORF information:

    RefSeq Version XM_021474394.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474394.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACAGGAAGC AGCAAAGGGA AAGACATCAG TACTATCAAG
    TCTCTACGTG TGCTTCGAGT ACTGCGGCCG CTGAAAACCA TCAAACGTCT GCCAAAACTG
    AAGGCTGTCT TTGACTGTGT GGTGAACTCT CTGAAGAATG TGTTGAACAT CCTCATCGTC
    TACATGCTCT TCATGTTCAT CTTCGCTGTG GTGGCTGTGC AGCTCTTTAA GGGACGTTTC
    TTTTACTGCA CTGATGAATC CAAAGAGTTT GAGCGCGACT GCAGGGGCGA GTATCTGGTC
    TATGAAAGAG ATAACGAAGT GAGGTCACAG AAGCGGGAAT GGAAGAAATA CGATTTCCAC
    TACGACAACG TGCTCTGGGC CCTTCTCACC CTCTTCACCG TTTCTACGGG AGAGGGGTGG
    CCGCAAGTGC TAAAACATTC AGTAGATGCG ACCTACGAGA ATCAGGGCCC GAGCCCAGGT
    TACCGGATGG AAATGTCCAT CTTTTACGTG GTGTACTTCG TGGTCTTCCC CTTCTTCTTC
    GTCAACATCT TCGTGGCTCT TATCATCATT ACTTTCCAGG AGCAAGGAGA CAAAATGATG
    GAGGACTATA GCCTGGAAAA GAATGAGAGA GCCTGCATAG ACTTTGCCAT CAACGCTAAG
    CCGCTCACAC GGCACATGCC CCAGAACAAG CAGACCTTTC AGTACCGCAT GTGGGAGTTT
    GTGGTGTCTC CTCCATTTGA ATACACCATC ATGGCCTTGA TCGCGCTCAA CACTATTGTC
    CTTATGATGA AGTATGATGG AGCATCGCTT ACTTATGAAG ATGTGCTAAA ATACCTGAAC
    ATAGTGTTCA CCTCTCTATT CTCCATGGAG TGCATATTGA AGATCATAGC CTTCGGAGCA
    TTGAACTACT TCAAAGATGC CTGGAACATC TTCGACTGTG TGACTGTACT TGGCAGCATC
    ACTGATATTT TAGTCACAGA GCTGGGGATG AACAACTTCA TAAATCTGAG TTTTCTAAGG
    CTCTTCAGAG CTGCTCGTCT CATTAAGTTG CTCAGGCAGG GTGAAACCAT ACGCATCCTG
    CTCTGGACTT TTGTCCAGTC TTTCAAGGCT CTGCCGTATG TGTGCCTACT CATCGCCATG
    CTGTTCTTCA TCTACGCCAT CATTGGCATG CAGCTGTTTG GGAACATTAA GATCGAGGAG
    AACAGTGACA GTGCCATTAC CCAGCACAAC AACTTCAGGA CTTTTTTCCA GGCCCTAATG
    CTGCTCTTTA GGAGTGCTAC AGGTGAGGCA TGGCATGACA TCATGCTGTC ATGTCTGGGA
    AAGAAGCCGT GTGATATTCT CTCTGATAAC CCGAAGCCAG AATGCGGGAG CGAGTTTGCC
    TACCTCTACT TTGTCTCCTT CATCTTCCTC TGTTCATTTC TGATGCTGAA TCTGTTCGTG
    GCTGTGATCA TGGATAATTT TGAATACCTG ACGCGTGACT CCTCTATCCT GGGACCCCAT
    CACCTGGACG AGTATGTGAG GATCTGGGCA GAATATGATC CTGCTGCTTG CGGGCGCATT
    CATTATAAGG ATATGTACAG TTTATTGCGA GTTATCGACC CCCCTCTGGG CTTAGGCAAG
    AAATGTCCTC ATAGGGTGGC CTGCAAGAGA CTGTTGCGGA TGGATCTGCC GGTTGCCGAT
    GACAACACAG TGCACTTTAA TTCTACCCTT ATGGCCTTAA TCCGAACCGC TCTGGACATC
    AAGATCGCCA AGGGTGGGGA AGGAGGTGTA GATAAGCACC AGATGGATGC TGAATTAAGG
    AAGGAGATGA TGGCGATATG GCCTAATCTC TCTCAGAAGA ACCTGGACCT GCTGGTGACT
    CCTCACAAGT CAGCGACAGA CCTGACAGTA GGAAAGATCT ACGCTGCCAT GATGATCATG
    GAGTATTATC GGCAAAGTAA AGCCAAACGG ACACAAGCTC TCCATGATGA GCAGAATCGA
    ACGCCATTAA TGTTTCAGCG TCTGGAGCCG CCATCTCCTA GCCAGGATGT GGGACCAGGA
    CTCACTGGCC TTCCTGACAC ACAAATGCAC CCTGCAAATC ATCTGCCTGT TGATGAAAGG
    ATCCCAGAGA GCCAATCATG GGTGACAGCG CGAGCACAGG AGATTTCTCA AAAAGCAGGC
    AACTGGAGTC CAGAGGGCCA ACATCCAGAT GACACCACAG ACAACAGAAG GCAGTCTCAG
    ACAGTAGAGA TGAGAGAGAT GGGGCGGGAC GGGTACTCGG ACACAGAGCA ATATCTGCCG
    ATGGAAGGCC ATGGCAGGGC TGCTTCCATG CCTCGACTCC CAGCAGACAA CCAACAGCCA
    CGGAGGAAAG GGCGTCACCG GGGAGAAAAT CTCACTCAGC CTATCACAGA TAACAGTCCT
    ATGAAGCGCT CGGCTTCATC TTTGGGACAC GGGCGTGCAG GACGCTCCAT GCGAGGTGAG
    GATTATGCCA TGGAGAGGGT TATCCCAGAA GAGGGTCACA GGCATGGGCA TCGCCACCGA
    GACCGTAGTC ACCGGGCGTC AGAACGCTCC CTGAGCCGAT ACACTGATGC TGACACAGGT
    CTTGGAACTG ACCTCAGCAC AACCACACAA TCAGGAGACC TGCCATCCAA GGAGAGAGAC
    CGGGGCCGAG CCAAAGACCG CAAGCACCAT CATCATCATC ATCACCACCA CGGCTCTTTA
    GATAAGGAGC ACTACGGTCA TGAGAGAGAC AGAGGAGAGT ACGGCCACAG ACAGTCCAGA
    GAGAGAGACC GCCGCTGGTC CCGCTCACCC AGTGAGGGCA GAGAGTGTCT GACTCACAGA
    CAGGGCAGTA GTTCAGTAAG CGGCAGTCCA GTCCCGTCCA CCTCCGGGAC CAGCACCCCC
    CGCCGTGGCC GCCGTCAGCT CCCCCAAACC CCTGCTACAC CCCGGCCACA TGTCACCTAC
    TCACCTGTGG TCCGTAAGCC CATCAGCAGT ACCCCCCCTC CTGGCCAGCA GAGCCGACTC
    CCTACCCCGA CCTCCCGGCG CTTCAGTCCG ACTGGCCCCG AGCCCCCGCT TCCCCCTCAT
    CACCACCCGT CACCACCCCA CCACGGCTCT CCCCGCTCCG GCCGACATGC ACACTGGGGC
    CCCGAGCCTG CAGAGAGCGT GGAGGGTGAC GGCTTCTATG ACGACCAAGA CTATGAGTTC
    AATCACCACG AGCCTCCATC CTACGAGCAA AGCCCTGTGC AGGGCAACCC TCACCCCCAT
    TCCCCTAGGA CTTCCCGCCA CAACACCCCT CCTCAGGGCC AAGCGCACCC ACGGCGCATG
    CCAAATGGCT ACCGTTCGTC TTCGCCCTCT CCACACCACC ACGCGCCCGC TCACCCTGGA
    ACTCATAAAC CTCCTCATCC CAGAGGGCCC AGAAAGGGCC TCCATGAACC CTACAGTGAG
    ACTGACGAGG ATGACTGGTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141791
    Clone ID Related Accession (Same CDS sequence) XM_021474398.1
    Accession Version XM_021474398.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7104bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGTGGCAGAA TCAGTTACAG GGATATGTAT GAGATGCTCA GGCACATGTC TCCTCCGTTA
    GGCCTGGGGA AGAAGTGCCC TGCACGGGTT GCCTACAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGAGGT GTAGATAAGC ACCAGATGGA TGCTGAATTA
    AGGAAGGAGA TGATGGCGAT ATGGCCTAAT CTCTCTCAGA AGAACCTGGA CCTGCTGGTG
    ACTCCTCACA AGTCGACAGA CCTGACAGTA GGAAAGATCT ACGCTGCCAT GATGATCATG
    GAGTATTATC GGCAAAGTAA AGCCAAACGG ACACAAGCTC TCCATGATGA GCAGAATCGA
    ACGCCATTAA TGTTTCAGCG TCTGGAGCCG CCATCTCCTA GCCAGGATGT GGGACCAGGA
    CTCACTGGCC TTCCTGACAC ACAAATGCAC CCTGCAAATC ATCTGCCTGT TGATGAAAGG
    ATCCCAGAGA GCCAATCATG GGTGACAGCG CGAGCACAGG AGATTTCTCA AAAAGCAGGC
    AACTGGAGTC CAGAGGGCCA ACATCCAGAT GACACCACAG ACAACAGAAG GCAGTCTCAG
    ACAGTAGAGA TGAGAGAGAT GGGGCGGGAC GGGTACTCGG ACACAGAGCA ATATCTGCCG
    ATGGAAGGCC ATGGCAGGGC TGCTTCCATG CCTCGACTCC CAGCAGACAA CCAACAGCCA
    CGGAGGAAAG GGCGTCACCG GGGAGAAAAT CTCACTCAGC CTATCACAGA TAACAGTCCT
    ATGAAGCGCT CGGCTTCATC TTTGGGACAC GGGCGTGCAG GACGCTCCAT GCGAGGTGAG
    GATTATGCCA TGGAGAGGGT TATCCCAGAA GAGGGTCACA GGCATGGGCA TCGCCACCGA
    GACCGTAGTC ACCGGGCGTC AGAACGCTCC CTGAGCCGAT ACACTGATGC TGACACAGGT
    CTTGGAACTG ACCTCAGCAC AACCACACAA TCAGGAGACC TGCCATCCAA GGAGAGAGAC
    CGGGGCCGAG CCAAAGACCG CAAGCACCAT CATCATCATC ATCACCACCA CGGCTCTTTA
    GATAAGGAGC ACTACGGTCA TGAGAGAGAC AGAGGAGAGT ACGGCCACAG ACAGTCCAGA
    GAGAGAGACC GCCGCTGGTC CCGCTCACCC AGTGAGGGCA GAGAGTGTCT GACTCACAGA
    CAGGGCAGTA GTTCAGTAAG CGGCAGTCCA GTCCCGTCCA CCTCCGGGAC CAGCACCCCC
    CGCCGTGGCC GCCGTCAGCT CCCCCAAACC CCTGCTACAC CCCGGCCACA TGTCACCTAC
    TCACCTGTGG TCCGTAAGCC CATCAGCAGT ACCCCCCCTC CTGGCCAGCA GAGCCGACTC
    CCTACCCCGA CCTCCCGGCG CTTCAGTCCG ACTGGCCCCG AGCCCCCGCT TCCCCCTCAT
    CACCACCCGT CACCACCCCA CCACGGCTCT CCCCGCTCCG GCCGACATGC ACACTGGGGC
    CCCGAGCCTG CAGAGAGCGT GGAGGGTGAC GGCTTCTATG ACGACCAAGA CTATGAGTTC
    AATCACCACG AGCCTCCATC CTACGAGCAA AGCCCTGTGC AGGGCAACCC TCACCCCCAT
    TCCCCTAGGA CTTCCCGCCA CAACACCCCT CCTCAGGGCC AAGCGCACCC ACGGCGCATG
    CCAAATGGCT ACCGTTCGTC TTCGCCCTCT CCACACCACC ACGCGCCCGC TCACCCTGGA
    ACTCATAAAC CTCCTCATCC CAGAGGGCCC AGAAAGGGCC TCCATGAACC CTACAGTGAG
    ACTGACGAGG ATGACTGGTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330073.1
    CDS1510..8613
    Translation

    Target ORF information:

    RefSeq Version XM_021474398.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474398.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGTGGCAGAA TCAGTTACAG GGATATGTAT GAGATGCTCA GGCACATGTC TCCTCCGTTA
    GGCCTGGGGA AGAAGTGCCC TGCACGGGTT GCCTACAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGAGGT GTAGATAAGC ACCAGATGGA TGCTGAATTA
    AGGAAGGAGA TGATGGCGAT ATGGCCTAAT CTCTCTCAGA AGAACCTGGA CCTGCTGGTG
    ACTCCTCACA AGTCGACAGA CCTGACAGTA GGAAAGATCT ACGCTGCCAT GATGATCATG
    GAGTATTATC GGCAAAGTAA AGCCAAACGG ACACAAGCTC TCCATGATGA GCAGAATCGA
    ACGCCATTAA TGTTTCAGCG TCTGGAGCCG CCATCTCCTA GCCAGGATGT GGGACCAGGA
    CTCACTGGCC TTCCTGACAC ACAAATGCAC CCTGCAAATC ATCTGCCTGT TGATGAAAGG
    ATCCCAGAGA GCCAATCATG GGTGACAGCG CGAGCACAGG AGATTTCTCA AAAAGCAGGC
    AACTGGAGTC CAGAGGGCCA ACATCCAGAT GACACCACAG ACAACAGAAG GCAGTCTCAG
    ACAGTAGAGA TGAGAGAGAT GGGGCGGGAC GGGTACTCGG ACACAGAGCA ATATCTGCCG
    ATGGAAGGCC ATGGCAGGGC TGCTTCCATG CCTCGACTCC CAGCAGACAA CCAACAGCCA
    CGGAGGAAAG GGCGTCACCG GGGAGAAAAT CTCACTCAGC CTATCACAGA TAACAGTCCT
    ATGAAGCGCT CGGCTTCATC TTTGGGACAC GGGCGTGCAG GACGCTCCAT GCGAGGTGAG
    GATTATGCCA TGGAGAGGGT TATCCCAGAA GAGGGTCACA GGCATGGGCA TCGCCACCGA
    GACCGTAGTC ACCGGGCGTC AGAACGCTCC CTGAGCCGAT ACACTGATGC TGACACAGGT
    CTTGGAACTG ACCTCAGCAC AACCACACAA TCAGGAGACC TGCCATCCAA GGAGAGAGAC
    CGGGGCCGAG CCAAAGACCG CAAGCACCAT CATCATCATC ATCACCACCA CGGCTCTTTA
    GATAAGGAGC ACTACGGTCA TGAGAGAGAC AGAGGAGAGT ACGGCCACAG ACAGTCCAGA
    GAGAGAGACC GCCGCTGGTC CCGCTCACCC AGTGAGGGCA GAGAGTGTCT GACTCACAGA
    CAGGGCAGTA GTTCAGTAAG CGGCAGTCCA GTCCCGTCCA CCTCCGGGAC CAGCACCCCC
    CGCCGTGGCC GCCGTCAGCT CCCCCAAACC CCTGCTACAC CCCGGCCACA TGTCACCTAC
    TCACCTGTGG TCCGTAAGCC CATCAGCAGT ACCCCCCCTC CTGGCCAGCA GAGCCGACTC
    CCTACCCCGA CCTCCCGGCG CTTCAGTCCG ACTGGCCCCG AGCCCCCGCT TCCCCCTCAT
    CACCACCCGT CACCACCCCA CCACGGCTCT CCCCGCTCCG GCCGACATGC ACACTGGGGC
    CCCGAGCCTG CAGAGAGCGT GGAGGGTGAC GGCTTCTATG ACGACCAAGA CTATGAGTTC
    AATCACCACG AGCCTCCATC CTACGAGCAA AGCCCTGTGC AGGGCAACCC TCACCCCCAT
    TCCCCTAGGA CTTCCCGCCA CAACACCCCT CCTCAGGGCC AAGCGCACCC ACGGCGCATG
    CCAAATGGCT ACCGTTCGTC TTCGCCCTCT CCACACCACC ACGCGCCCGC TCACCCTGGA
    ACTCATAAAC CTCCTCATCC CAGAGGGCCC AGAAAGGGCC TCCATGAACC CTACAGTGAG
    ACTGACGAGG ATGACTGGTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141792
    Clone ID Related Accession (Same CDS sequence) XM_021474403.1
    Accession Version XM_021474403.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7101bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA ACAACTTCAT AAATCTGAGT
    TTTCTAAGGC TCTTCAGAGC TGCTCGTCTC ATTAAGTTGC TCAGGCAGGG TGAAACCATA
    CGCATCCTGC TCTGGACTTT TGTCCAGTCT TTCAAGGCTC TGCCGTATGT GTGCCTACTC
    ATCGCCATGC TGTTCTTCAT CTACGCCATC ATTGGCATGC AGCTGTTTGG GAACATTAAG
    ATCGAGGAGA ACAGTGACAG TGCCATTACC CAGCACAACA ACTTCAGGAC TTTTTTCCAG
    GCCCTAATGC TGCTCTTTAG GAGTGCTACA GGTGAGGCAT GGCATGACAT CATGCTGTCA
    TGTCTGGGAA AGAAGCCGTG TGATATTCTC TCTGATAACC CGAAGCCAGA ATGCGGGAGC
    GAGTTTGCCT ACCTCTACTT TGTCTCCTTC ATCTTCCTCT GTTCATTTCT GATGCTGAAT
    CTGTTCGTGG CTGTGATCAT GGATAATTTT GAATACCTGA CGCGTGACTC CTCTATCCTG
    GGACCCCATC ACCTGGACGA GTATGTGAGG ATCTGGGCAG AATATGATCC TGCTGCTTGT
    GGCAGAATCA GTTACAGGGA TATGTATGAG ATGCTCAGGC ACATGTCTCC TCCGTTAGGC
    CTGGGGAAGA AGTGCCCTGC ACGGGTTGCC TACAAGAGAC TGTTGCGGAT GGATCTGCCG
    GTTGCCGATG ACAACACAGT GCACTTTAAT TCTACCCTTA TGGCCTTAAT CCGAACCGCT
    CTGGACATCA AGATCGCCAA GGGAGGTGTA GATAAGCACC AGATGGATGC TGAATTAAGG
    AAGGAGATGA TGGCGATATG GCCTAATCTC TCTCAGAAGA ACCTGGACCT GCTGGTGACT
    CCTCACAAGT CGACAGACCT GACAGTAGGA AAGATCTACG CTGCCATGAT GATCATGGAG
    TATTATCGGC AAAGTAAAGC CAAACGGACA CAAGCTCTCC ATGATGAGCA GAATCGAACG
    CCATTAATGT TTCAGCGTCT GGAGCCGCCA TCTCCTAGCC AGGATGTGGG ACCAGGACTC
    ACTGGCCTTC CTGACACACA AATGCACCCT GCAAATCATC TGCCTGTTGA TGAAAGGATC
    CCAGAGAGCC AATCATGGGT GACAGCGCGA GCACAGGAGA TTTCTCAAAA AGCAGGCAAC
    TGGAGTCCAG AGGGCCAACA TCCAGATGAC ACCACAGACA ACAGAAGGCA GTCTCAGACA
    GTAGAGATGA GAGAGATGGG GCGGGACGGG TACTCGGACA CAGAGCAATA TCTGCCGATG
    GAAGGCCATG GCAGGGCTGC TTCCATGCCT CGACTCCCAG CAGACAACCA ACAGCCACGG
    AGGAAAGGGC GTCACCGGGG AGAAAATCTC ACTCAGCCTA TCACAGATAA CAGTCCTATG
    AAGCGCTCGG CTTCATCTTT GGGACACGGG CGTGCAGGAC GCTCCATGCG AGGTGAGGAT
    TATGCCATGG AGAGGGTTAT CCCAGAAGAG GGTCACAGGC ATGGGCATCG CCACCGAGAC
    CGTAGTCACC GGGCGTCAGA ACGCTCCCTG AGCCGATACA CTGATGCTGA CACAGGTCTT
    GGAACTGACC TCAGCACAAC CACACAATCA GGAGACCTGC CATCCAAGGA GAGAGACCGG
    GGCCGAGCCA AAGACCGCAA GCACCATCAT CATCATCATC ACCACCACGG CTCTTTAGAT
    AAGGAGCACT ACGGTCATGA GAGAGACAGA GGAGAGTACG GCCACAGACA GTCCAGAGAG
    AGAGACCGCC GCTGGTCCCG CTCACCCAGT GAGGGCAGAG AGTGTCTGAC TCACAGACAG
    GGCAGTAGTT CAGTAAGCGG CAGTCCAGTC CCGTCCACCT CCGGGACCAG CACCCCCCGC
    CGTGGCCGCC GTCAGCTCCC CCAAACCCCT GCTACACCCC GGCCACATGT CACCTACTCA
    CCTGTGGTCC GTAAGCCCAT CAGCAGTACC CCCCCTCCTG GCCAGCAGAG CCGACTCCCT
    ACCCCGACCT CCCGGCGCTT CAGTCCGACT GGCCCCGAGC CCCCGCTTCC CCCTCATCAC
    CACCCGTCAC CACCCCACCA CGGCTCTCCC CGCTCCGGCC GACATGCACA CTGGGGCCCC
    GAGCCTGCAG AGAGCGTGGA GGGTGACGGC TTCTATGACG ACCAAGACTA TGAGTTCAAT
    CACCACGAGC CTCCATCCTA CGAGCAAAGC CCTGTGCAGG GCAACCCTCA CCCCCATTCC
    CCTAGGACTT CCCGCCACAA CACCCCTCCT CAGGGCCAAG CGCACCCACG GCGCATGCCA
    AATGGCTACC GTTCGTCTTC GCCCTCTCCA CACCACCACG CGCCCGCTCA CCCTGGAACT
    CATAAACCTC CTCATCCCAG AGGGCCCAGA AAGGGCCTCC ATGAACCCTA CAGTGAGACT
    GACGAGGATG ACTGGTGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330078.1
    CDS1510..8610
    Translation

    Target ORF information:

    RefSeq Version XM_021474403.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474403.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA ACAACTTCAT AAATCTGAGT
    TTTCTAAGGC TCTTCAGAGC TGCTCGTCTC ATTAAGTTGC TCAGGCAGGG TGAAACCATA
    CGCATCCTGC TCTGGACTTT TGTCCAGTCT TTCAAGGCTC TGCCGTATGT GTGCCTACTC
    ATCGCCATGC TGTTCTTCAT CTACGCCATC ATTGGCATGC AGCTGTTTGG GAACATTAAG
    ATCGAGGAGA ACAGTGACAG TGCCATTACC CAGCACAACA ACTTCAGGAC TTTTTTCCAG
    GCCCTAATGC TGCTCTTTAG GAGTGCTACA GGTGAGGCAT GGCATGACAT CATGCTGTCA
    TGTCTGGGAA AGAAGCCGTG TGATATTCTC TCTGATAACC CGAAGCCAGA ATGCGGGAGC
    GAGTTTGCCT ACCTCTACTT TGTCTCCTTC ATCTTCCTCT GTTCATTTCT GATGCTGAAT
    CTGTTCGTGG CTGTGATCAT GGATAATTTT GAATACCTGA CGCGTGACTC CTCTATCCTG
    GGACCCCATC ACCTGGACGA GTATGTGAGG ATCTGGGCAG AATATGATCC TGCTGCTTGT
    GGCAGAATCA GTTACAGGGA TATGTATGAG ATGCTCAGGC ACATGTCTCC TCCGTTAGGC
    CTGGGGAAGA AGTGCCCTGC ACGGGTTGCC TACAAGAGAC TGTTGCGGAT GGATCTGCCG
    GTTGCCGATG ACAACACAGT GCACTTTAAT TCTACCCTTA TGGCCTTAAT CCGAACCGCT
    CTGGACATCA AGATCGCCAA GGGAGGTGTA GATAAGCACC AGATGGATGC TGAATTAAGG
    AAGGAGATGA TGGCGATATG GCCTAATCTC TCTCAGAAGA ACCTGGACCT GCTGGTGACT
    CCTCACAAGT CGACAGACCT GACAGTAGGA AAGATCTACG CTGCCATGAT GATCATGGAG
    TATTATCGGC AAAGTAAAGC CAAACGGACA CAAGCTCTCC ATGATGAGCA GAATCGAACG
    CCATTAATGT TTCAGCGTCT GGAGCCGCCA TCTCCTAGCC AGGATGTGGG ACCAGGACTC
    ACTGGCCTTC CTGACACACA AATGCACCCT GCAAATCATC TGCCTGTTGA TGAAAGGATC
    CCAGAGAGCC AATCATGGGT GACAGCGCGA GCACAGGAGA TTTCTCAAAA AGCAGGCAAC
    TGGAGTCCAG AGGGCCAACA TCCAGATGAC ACCACAGACA ACAGAAGGCA GTCTCAGACA
    GTAGAGATGA GAGAGATGGG GCGGGACGGG TACTCGGACA CAGAGCAATA TCTGCCGATG
    GAAGGCCATG GCAGGGCTGC TTCCATGCCT CGACTCCCAG CAGACAACCA ACAGCCACGG
    AGGAAAGGGC GTCACCGGGG AGAAAATCTC ACTCAGCCTA TCACAGATAA CAGTCCTATG
    AAGCGCTCGG CTTCATCTTT GGGACACGGG CGTGCAGGAC GCTCCATGCG AGGTGAGGAT
    TATGCCATGG AGAGGGTTAT CCCAGAAGAG GGTCACAGGC ATGGGCATCG CCACCGAGAC
    CGTAGTCACC GGGCGTCAGA ACGCTCCCTG AGCCGATACA CTGATGCTGA CACAGGTCTT
    GGAACTGACC TCAGCACAAC CACACAATCA GGAGACCTGC CATCCAAGGA GAGAGACCGG
    GGCCGAGCCA AAGACCGCAA GCACCATCAT CATCATCATC ACCACCACGG CTCTTTAGAT
    AAGGAGCACT ACGGTCATGA GAGAGACAGA GGAGAGTACG GCCACAGACA GTCCAGAGAG
    AGAGACCGCC GCTGGTCCCG CTCACCCAGT GAGGGCAGAG AGTGTCTGAC TCACAGACAG
    GGCAGTAGTT CAGTAAGCGG CAGTCCAGTC CCGTCCACCT CCGGGACCAG CACCCCCCGC
    CGTGGCCGCC GTCAGCTCCC CCAAACCCCT GCTACACCCC GGCCACATGT CACCTACTCA
    CCTGTGGTCC GTAAGCCCAT CAGCAGTACC CCCCCTCCTG GCCAGCAGAG CCGACTCCCT
    ACCCCGACCT CCCGGCGCTT CAGTCCGACT GGCCCCGAGC CCCCGCTTCC CCCTCATCAC
    CACCCGTCAC CACCCCACCA CGGCTCTCCC CGCTCCGGCC GACATGCACA CTGGGGCCCC
    GAGCCTGCAG AGAGCGTGGA GGGTGACGGC TTCTATGACG ACCAAGACTA TGAGTTCAAT
    CACCACGAGC CTCCATCCTA CGAGCAAAGC CCTGTGCAGG GCAACCCTCA CCCCCATTCC
    CCTAGGACTT CCCGCCACAA CACCCCTCCT CAGGGCCAAG CGCACCCACG GCGCATGCCA
    AATGGCTACC GTTCGTCTTC GCCCTCTCCA CACCACCACG CGCCCGCTCA CCCTGGAACT
    CATAAACCTC CTCATCCCAG AGGGCCCAGA AAGGGCCTCC ATGAACCCTA CAGTGAGACT
    GACGAGGATG ACTGGTGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141793
    Clone ID Related Accession (Same CDS sequence) XM_021474404.1
    Accession Version XM_021474404.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7092bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG
    GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT
    GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC
    CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC
    ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT
    GAGCAGCGTC TGGAGCCGCC ATCTCCTAGC CAGGATGTGG GACCAGGACT CACTGGCCTT
    CCTGACACAC AAATGCACCC TGCAAATCAT CTGCCTGTTG ATGAAAGGAT CCCAGAGAGC
    CAATCATGGG TGACAGCGCG AGCACAGGAG ATTTCTCAAA AAGCAGGCAA CTGGAGTCCA
    GAGGGCCAAC ATCCAGATGA CACCACAGAC AACAGAAGGC AGTCTCAGAC AGTAGAGATG
    AGAGAGATGG GGCGGGACGG GTACTCGGAC ACAGAGCAAT ATCTGCCGAT GGAAGGCCAT
    GGCAGGGCTG CTTCCATGCC TCGACTCCCA GCAGACAACC AACAGCCACG GAGGAAAGGG
    CGTCACCGGG GAGAAAATCT CACTCAGCCT ATCACAGATA ACAGTCCTAT GAAGCGCTCG
    GCTTCATCTT TGGGACACGG GCGTGCAGGA CGCTCCATGC GAGGTGAGGA TTATGCCATG
    GAGAGGGTTA TCCCAGAAGA GGGTCACAGG CATGGGCATC GCCACCGAGA CCGTAGTCAC
    CGGGCGTCAG AACGCTCCCT GAGCCGATAC ACTGATGCTG ACACAGGTCT TGGAACTGAC
    CTCAGCACAA CCACACAATC AGGAGACCTG CCATCCAAGG AGAGAGACCG GGGCCGAGCC
    AAAGACCGCA AGCACCATCA TCATCATCAT CACCACCACG GCTCTTTAGA TAAGGAGCAC
    TACGGTCATG AGAGAGACAG AGGAGAGTAC GGCCACAGAC AGTCCAGAGA GAGAGACCGC
    CGCTGGTCCC GCTCACCCAG TGAGGGCAGA GAGTGTCTGA CTCACAGACA GGGCAGTAGT
    TCAGTAAGCG GCAGTCCAGT CCCGTCCACC TCCGGGACCA GCACCCCCCG CCGTGGCCGC
    CGTCAGCTCC CCCAAACCCC TGCTACACCC CGGCCACATG TCACCTACTC ACCTGTGGTC
    CGTAAGCCCA TCAGCAGTAC CCCCCCTCCT GGCCAGCAGA GCCGACTCCC TACCCCGACC
    TCCCGGCGCT TCAGTCCGAC TGGCCCCGAG CCCCCGCTTC CCCCTCATCA CCACCCGTCA
    CCACCCCACC ACGGCTCTCC CCGCTCCGGC CGACATGCAC ACTGGGGCCC CGAGCCTGCA
    GAGAGCGTGG AGGGTGACGG CTTCTATGAC GACCAAGACT ATGAGTTCAA TCACCACGAG
    CCTCCATCCT ACGAGCAAAG CCCTGTGCAG GGCAACCCTC ACCCCCATTC CCCTAGGACT
    TCCCGCCACA ACACCCCTCC TCAGGGCCAA GCGCACCCAC GGCGCATGCC AAATGGCTAC
    CGTTCGTCTT CGCCCTCTCC ACACCACCAC GCGCCCGCTC ACCCTGGAAC TCATAAACCT
    CCTCATCCCA GAGGGCCCAG AAAGGGCCTC CATGAACCCT ACAGTGAGAC TGACGAGGAT
    GACTGGTGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330079.1
    CDS1508..8599
    Translation

    Target ORF information:

    RefSeq Version XM_021474404.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474404.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG
    GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT
    GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC
    CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC
    ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT
    GAGCAGCGTC TGGAGCCGCC ATCTCCTAGC CAGGATGTGG GACCAGGACT CACTGGCCTT
    CCTGACACAC AAATGCACCC TGCAAATCAT CTGCCTGTTG ATGAAAGGAT CCCAGAGAGC
    CAATCATGGG TGACAGCGCG AGCACAGGAG ATTTCTCAAA AAGCAGGCAA CTGGAGTCCA
    GAGGGCCAAC ATCCAGATGA CACCACAGAC AACAGAAGGC AGTCTCAGAC AGTAGAGATG
    AGAGAGATGG GGCGGGACGG GTACTCGGAC ACAGAGCAAT ATCTGCCGAT GGAAGGCCAT
    GGCAGGGCTG CTTCCATGCC TCGACTCCCA GCAGACAACC AACAGCCACG GAGGAAAGGG
    CGTCACCGGG GAGAAAATCT CACTCAGCCT ATCACAGATA ACAGTCCTAT GAAGCGCTCG
    GCTTCATCTT TGGGACACGG GCGTGCAGGA CGCTCCATGC GAGGTGAGGA TTATGCCATG
    GAGAGGGTTA TCCCAGAAGA GGGTCACAGG CATGGGCATC GCCACCGAGA CCGTAGTCAC
    CGGGCGTCAG AACGCTCCCT GAGCCGATAC ACTGATGCTG ACACAGGTCT TGGAACTGAC
    CTCAGCACAA CCACACAATC AGGAGACCTG CCATCCAAGG AGAGAGACCG GGGCCGAGCC
    AAAGACCGCA AGCACCATCA TCATCATCAT CACCACCACG GCTCTTTAGA TAAGGAGCAC
    TACGGTCATG AGAGAGACAG AGGAGAGTAC GGCCACAGAC AGTCCAGAGA GAGAGACCGC
    CGCTGGTCCC GCTCACCCAG TGAGGGCAGA GAGTGTCTGA CTCACAGACA GGGCAGTAGT
    TCAGTAAGCG GCAGTCCAGT CCCGTCCACC TCCGGGACCA GCACCCCCCG CCGTGGCCGC
    CGTCAGCTCC CCCAAACCCC TGCTACACCC CGGCCACATG TCACCTACTC ACCTGTGGTC
    CGTAAGCCCA TCAGCAGTAC CCCCCCTCCT GGCCAGCAGA GCCGACTCCC TACCCCGACC
    TCCCGGCGCT TCAGTCCGAC TGGCCCCGAG CCCCCGCTTC CCCCTCATCA CCACCCGTCA
    CCACCCCACC ACGGCTCTCC CCGCTCCGGC CGACATGCAC ACTGGGGCCC CGAGCCTGCA
    GAGAGCGTGG AGGGTGACGG CTTCTATGAC GACCAAGACT ATGAGTTCAA TCACCACGAG
    CCTCCATCCT ACGAGCAAAG CCCTGTGCAG GGCAACCCTC ACCCCCATTC CCCTAGGACT
    TCCCGCCACA ACACCCCTCC TCAGGGCCAA GCGCACCCAC GGCGCATGCC AAATGGCTAC
    CGTTCGTCTT CGCCCTCTCC ACACCACCAC GCGCCCGCTC ACCCTGGAAC TCATAAACCT
    CCTCATCCCA GAGGGCCCAG AAAGGGCCTC CATGAACCCT ACAGTGAGAC TGACGAGGAT
    GACTGGTGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141794
    Clone ID Related Accession (Same CDS sequence) XM_021474408.1
    Accession Version XM_021474408.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7089bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACAGGAAGC AGCAAAGGGA AAGACATCAG TACTATCAAG
    TCTCTACGTG TGCTTCGAGT ACTGCGGCCG CTGAAAACCA TCAAACGTCT GCCAAAACTG
    AAGGCTGTCT TTGACTGTGT GGTGAACTCT CTGAAGAATG TGTTGAACAT CCTCATCGTC
    TACATGCTCT TCATGTTCAT CTTCGCTGTG GTGGCTGTGC AGCTCTTTAA GGGACGTTTC
    TTTTACTGCA CTGATGAATC CAAAGAGTTT GAGCGCGACT GCAGGGGCGA GTATCTGGTC
    TATGAAAGAG ATAACGAAGT GAGGTCACAG AAGCGGGAAT GGAAGAAATA CGATTTCCAC
    TACGACAACG TGCTCTGGGC CCTTCTCACC CTCTTCACCG TTTCTACGGG AGAGGGGTGG
    CCGCAAGTGC TAAAACATTC AGTAGATGCG ACCTACGAGA ATCAGGGCCC GAGCCCAGGT
    TACCGGATGG AAATGTCCAT CTTTTACGTG GTGTACTTCG TGGTCTTCCC CTTCTTCTTC
    GTCAACATCT TCGTGGCTCT TATCATCATT ACTTTCCAGG AGCAAGGAGA CAAAATGATG
    GAGGACTATA GCCTGGAAAA GAATGAGAGA GCCTGCATAG ACTTTGCCAT CAACGCTAAG
    CCGCTCACAC GGCACATGCC CCAGAACAAG CAGACCTTTC AGTACCGCAT GTGGGAGTTT
    GTGGTGTCTC CTCCATTTGA ATACACCATC ATGGCCTTGA TCGCGCTCAA CACTATTGTC
    CTTATGATGA AGTATGATGG AGCATCGCTT ACTTATGAAG ATGTGCTAAA ATACCTGAAC
    ATAGTGTTCA CCTCTCTATT CTCCATGGAG TGCATATTGA AGATCATAGC CTTCGGAGCA
    TTGAACTACT TCAAAGATGC CTGGAACATC TTCGACTGTG TGACTGTACT TGGCAGCATC
    ACTGATATTT TAGTCACAGA GCTGGGGAAC AACTTCATAA ATCTGAGTTT TCTAAGGCTC
    TTCAGAGCTG CTCGTCTCAT TAAGTTGCTC AGGCAGGGTG AAACCATACG CATCCTGCTC
    TGGACTTTTG TCCAGTCTTT CAAGGCTCTG CCGTATGTGT GCCTACTCAT CGCCATGCTG
    TTCTTCATCT ACGCCATCAT TGGCATGCAG CTGTTTGGGA ACATTAAGAT CGAGGAGAAC
    AGTGACAGTG CCATTACCCA GCACAACAAC TTCAGGACTT TTTTCCAGGC CCTAATGCTG
    CTCTTTAGGA GTGCTACAGG TGAGGCATGG CATGACATCA TGCTGTCATG TCTGGGAAAG
    AAGCCGTGTG ATATTCTCTC TGATAACCCG AAGCCAGAAT GCGGGAGCGA GTTTGCCTAC
    CTCTACTTTG TCTCCTTCAT CTTCCTCTGT TCATTTCTGA TGCTGAATCT GTTCGTGGCT
    GTGATCATGG ATAATTTTGA ATACCTGACG CGTGACTCCT CTATCCTGGG ACCCCATCAC
    CTGGACGAGT ATGTGAGGAT CTGGGCAGAA TATGATCCTG CTGCTTGCGG GCGCATTCAT
    TATAAGGATA TGTACAGTTT ATTGCGAGTT ATCGACCCCC CTCTGGGCTT AGGCAAGAAA
    TGTCCTCATA GGGTGGCCTG CAAGAGACTG TTGCGGATGG ATCTGCCGGT TGCCGATGAC
    AACACAGTGC ACTTTAATTC TACCCTTATG GCCTTAATCC GAACCGCTCT GGACATCAAG
    ATCGCCAAGG GAGGTGTAGA TAAGCACCAG ATGGATGCTG AATTAAGGAA GGAGATGATG
    GCGATATGGC CTAATCTCTC TCAGAAGAAC CTGGACCTGC TGGTGACTCC TCACAAGTCG
    ACAGACCTGA CAGTAGGAAA GATCTACGCT GCCATGATGA TCATGGAGTA TTATCGGCAA
    AGTAAAGCCA AACGGACACA AGCTCTCCAT GATGAGCAGA ATCGAACGCC ATTAATGTTT
    CAGCGTCTGG AGCCGCCATC TCCTAGCCAG GATGTGGGAC CAGGACTCAC TGGCCTTCCT
    GACACACAAA TGCACCCTGC AAATCATCTG CCTGTTGATG AAAGGATCCC AGAGAGCCAA
    TCATGGGTGA CAGCGCGAGC ACAGGAGATT TCTCAAAAAG CAGGCAACTG GAGTCCAGAG
    GGCCAACATC CAGATGACAC CACAGACAAC AGAAGGCAGT CTCAGACAGT AGAGATGAGA
    GAGATGGGGC GGGACGGGTA CTCGGACACA GAGCAATATC TGCCGATGGA AGGCCATGGC
    AGGGCTGCTT CCATGCCTCG ACTCCCAGCA GACAACCAAC AGCCACGGAG GAAAGGGCGT
    CACCGGGGAG AAAATCTCAC TCAGCCTATC ACAGATAACA GTCCTATGAA GCGCTCGGCT
    TCATCTTTGG GACACGGGCG TGCAGGACGC TCCATGCGAG GTGAGGATTA TGCCATGGAG
    AGGGTTATCC CAGAAGAGGG TCACAGGCAT GGGCATCGCC ACCGAGACCG TAGTCACCGG
    GCGTCAGAAC GCTCCCTGAG CCGATACACT GATGCTGACA CAGGTCTTGG AACTGACCTC
    AGCACAACCA CACAATCAGG AGACCTGCCA TCCAAGGAGA GAGACCGGGG CCGAGCCAAA
    GACCGCAAGC ACCATCATCA TCATCATCAC CACCACGGCT CTTTAGATAA GGAGCACTAC
    GGTCATGAGA GAGACAGAGG AGAGTACGGC CACAGACAGT CCAGAGAGAG AGACCGCCGC
    TGGTCCCGCT CACCCAGTGA GGGCAGAGAG TGTCTGACTC ACAGACAGGG CAGTAGTTCA
    GTAAGCGGCA GTCCAGTCCC GTCCACCTCC GGGACCAGCA CCCCCCGCCG TGGCCGCCGT
    CAGCTCCCCC AAACCCCTGC TACACCCCGG CCACATGTCA CCTACTCACC TGTGGTCCGT
    AAGCCCATCA GCAGTACCCC CCCTCCTGGC CAGCAGAGCC GACTCCCTAC CCCGACCTCC
    CGGCGCTTCA GTCCGACTGG CCCCGAGCCC CCGCTTCCCC CTCATCACCA CCCGTCACCA
    CCCCACCACG GCTCTCCCCG CTCCGGCCGA CATGCACACT GGGGCCCCGA GCCTGCAGAG
    AGCGTGGAGG GTGACGGCTT CTATGACGAC CAAGACTATG AGTTCAATCA CCACGAGCCT
    CCATCCTACG AGCAAAGCCC TGTGCAGGGC AACCCTCACC CCCATTCCCC TAGGACTTCC
    CGCCACAACA CCCCTCCTCA GGGCCAAGCG CACCCACGGC GCATGCCAAA TGGCTACCGT
    TCGTCTTCGC CCTCTCCACA CCACCACGCG CCCGCTCACC CTGGAACTCA TAAACCTCCT
    CATCCCAGAG GGCCCAGAAA GGGCCTCCAT GAACCCTACA GTGAGACTGA CGAGGATGAC
    TGGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330083.1
    CDS1509..8597
    Translation

    Target ORF information:

    RefSeq Version XM_021474408.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474408.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACAGGAAGC AGCAAAGGGA AAGACATCAG TACTATCAAG
    TCTCTACGTG TGCTTCGAGT ACTGCGGCCG CTGAAAACCA TCAAACGTCT GCCAAAACTG
    AAGGCTGTCT TTGACTGTGT GGTGAACTCT CTGAAGAATG TGTTGAACAT CCTCATCGTC
    TACATGCTCT TCATGTTCAT CTTCGCTGTG GTGGCTGTGC AGCTCTTTAA GGGACGTTTC
    TTTTACTGCA CTGATGAATC CAAAGAGTTT GAGCGCGACT GCAGGGGCGA GTATCTGGTC
    TATGAAAGAG ATAACGAAGT GAGGTCACAG AAGCGGGAAT GGAAGAAATA CGATTTCCAC
    TACGACAACG TGCTCTGGGC CCTTCTCACC CTCTTCACCG TTTCTACGGG AGAGGGGTGG
    CCGCAAGTGC TAAAACATTC AGTAGATGCG ACCTACGAGA ATCAGGGCCC GAGCCCAGGT
    TACCGGATGG AAATGTCCAT CTTTTACGTG GTGTACTTCG TGGTCTTCCC CTTCTTCTTC
    GTCAACATCT TCGTGGCTCT TATCATCATT ACTTTCCAGG AGCAAGGAGA CAAAATGATG
    GAGGACTATA GCCTGGAAAA GAATGAGAGA GCCTGCATAG ACTTTGCCAT CAACGCTAAG
    CCGCTCACAC GGCACATGCC CCAGAACAAG CAGACCTTTC AGTACCGCAT GTGGGAGTTT
    GTGGTGTCTC CTCCATTTGA ATACACCATC ATGGCCTTGA TCGCGCTCAA CACTATTGTC
    CTTATGATGA AGTATGATGG AGCATCGCTT ACTTATGAAG ATGTGCTAAA ATACCTGAAC
    ATAGTGTTCA CCTCTCTATT CTCCATGGAG TGCATATTGA AGATCATAGC CTTCGGAGCA
    TTGAACTACT TCAAAGATGC CTGGAACATC TTCGACTGTG TGACTGTACT TGGCAGCATC
    ACTGATATTT TAGTCACAGA GCTGGGGAAC AACTTCATAA ATCTGAGTTT TCTAAGGCTC
    TTCAGAGCTG CTCGTCTCAT TAAGTTGCTC AGGCAGGGTG AAACCATACG CATCCTGCTC
    TGGACTTTTG TCCAGTCTTT CAAGGCTCTG CCGTATGTGT GCCTACTCAT CGCCATGCTG
    TTCTTCATCT ACGCCATCAT TGGCATGCAG CTGTTTGGGA ACATTAAGAT CGAGGAGAAC
    AGTGACAGTG CCATTACCCA GCACAACAAC TTCAGGACTT TTTTCCAGGC CCTAATGCTG
    CTCTTTAGGA GTGCTACAGG TGAGGCATGG CATGACATCA TGCTGTCATG TCTGGGAAAG
    AAGCCGTGTG ATATTCTCTC TGATAACCCG AAGCCAGAAT GCGGGAGCGA GTTTGCCTAC
    CTCTACTTTG TCTCCTTCAT CTTCCTCTGT TCATTTCTGA TGCTGAATCT GTTCGTGGCT
    GTGATCATGG ATAATTTTGA ATACCTGACG CGTGACTCCT CTATCCTGGG ACCCCATCAC
    CTGGACGAGT ATGTGAGGAT CTGGGCAGAA TATGATCCTG CTGCTTGCGG GCGCATTCAT
    TATAAGGATA TGTACAGTTT ATTGCGAGTT ATCGACCCCC CTCTGGGCTT AGGCAAGAAA
    TGTCCTCATA GGGTGGCCTG CAAGAGACTG TTGCGGATGG ATCTGCCGGT TGCCGATGAC
    AACACAGTGC ACTTTAATTC TACCCTTATG GCCTTAATCC GAACCGCTCT GGACATCAAG
    ATCGCCAAGG GAGGTGTAGA TAAGCACCAG ATGGATGCTG AATTAAGGAA GGAGATGATG
    GCGATATGGC CTAATCTCTC TCAGAAGAAC CTGGACCTGC TGGTGACTCC TCACAAGTCG
    ACAGACCTGA CAGTAGGAAA GATCTACGCT GCCATGATGA TCATGGAGTA TTATCGGCAA
    AGTAAAGCCA AACGGACACA AGCTCTCCAT GATGAGCAGA ATCGAACGCC ATTAATGTTT
    CAGCGTCTGG AGCCGCCATC TCCTAGCCAG GATGTGGGAC CAGGACTCAC TGGCCTTCCT
    GACACACAAA TGCACCCTGC AAATCATCTG CCTGTTGATG AAAGGATCCC AGAGAGCCAA
    TCATGGGTGA CAGCGCGAGC ACAGGAGATT TCTCAAAAAG CAGGCAACTG GAGTCCAGAG
    GGCCAACATC CAGATGACAC CACAGACAAC AGAAGGCAGT CTCAGACAGT AGAGATGAGA
    GAGATGGGGC GGGACGGGTA CTCGGACACA GAGCAATATC TGCCGATGGA AGGCCATGGC
    AGGGCTGCTT CCATGCCTCG ACTCCCAGCA GACAACCAAC AGCCACGGAG GAAAGGGCGT
    CACCGGGGAG AAAATCTCAC TCAGCCTATC ACAGATAACA GTCCTATGAA GCGCTCGGCT
    TCATCTTTGG GACACGGGCG TGCAGGACGC TCCATGCGAG GTGAGGATTA TGCCATGGAG
    AGGGTTATCC CAGAAGAGGG TCACAGGCAT GGGCATCGCC ACCGAGACCG TAGTCACCGG
    GCGTCAGAAC GCTCCCTGAG CCGATACACT GATGCTGACA CAGGTCTTGG AACTGACCTC
    AGCACAACCA CACAATCAGG AGACCTGCCA TCCAAGGAGA GAGACCGGGG CCGAGCCAAA
    GACCGCAAGC ACCATCATCA TCATCATCAC CACCACGGCT CTTTAGATAA GGAGCACTAC
    GGTCATGAGA GAGACAGAGG AGAGTACGGC CACAGACAGT CCAGAGAGAG AGACCGCCGC
    TGGTCCCGCT CACCCAGTGA GGGCAGAGAG TGTCTGACTC ACAGACAGGG CAGTAGTTCA
    GTAAGCGGCA GTCCAGTCCC GTCCACCTCC GGGACCAGCA CCCCCCGCCG TGGCCGCCGT
    CAGCTCCCCC AAACCCCTGC TACACCCCGG CCACATGTCA CCTACTCACC TGTGGTCCGT
    AAGCCCATCA GCAGTACCCC CCCTCCTGGC CAGCAGAGCC GACTCCCTAC CCCGACCTCC
    CGGCGCTTCA GTCCGACTGG CCCCGAGCCC CCGCTTCCCC CTCATCACCA CCCGTCACCA
    CCCCACCACG GCTCTCCCCG CTCCGGCCGA CATGCACACT GGGGCCCCGA GCCTGCAGAG
    AGCGTGGAGG GTGACGGCTT CTATGACGAC CAAGACTATG AGTTCAATCA CCACGAGCCT
    CCATCCTACG AGCAAAGCCC TGTGCAGGGC AACCCTCACC CCCATTCCCC TAGGACTTCC
    CGCCACAACA CCCCTCCTCA GGGCCAAGCG CACCCACGGC GCATGCCAAA TGGCTACCGT
    TCGTCTTCGC CCTCTCCACA CCACCACGCG CCCGCTCACC CTGGAACTCA TAAACCTCCT
    CATCCCAGAG GGCCCAGAAA GGGCCTCCAT GAACCCTACA GTGAGACTGA CGAGGATGAC
    TGGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141795
    Clone ID Related Accession (Same CDS sequence) XM_021474412.1
    Accession Version XM_021474412.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7089bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACAGGAAGC AGCAAAGGGA AAGACATCAG TACTATCAAG
    TCTCTACGTG TGCTTCGAGT ACTGCGGCCG CTGAAAACCA TCAAACGTCT GCCAAAACTG
    AAGGCTGTCT TTGACTGTGT GGTGAACTCT CTGAAGAATG TGTTGAACAT CCTCATCGTC
    TACATGCTCT TCATGTTCAT CTTCGCTGTG GTGGCTGTGC AGCTCTTTAA GGGACGTTTC
    TTTTACTGCA CTGATGAATC CAAAGAGTTT GAGCGCGACT GCAGGGGCGA GTATCTGGTC
    TATGAAAGAG ATAACGAAGT GAGGTCACAG AAGCGGGAAT GGAAGAAATA CGATTTCCAC
    TACGACAACG TGCTCTGGGC CCTTCTCACC CTCTTCACCG TTTCTACGGG AGAGGGGTGG
    CCGCAAGTGC TAAAACATTC AGTAGATGCG ACCTACGAGA ATCAGGGCCC GAGCCCAGGT
    TACCGGATGG AAATGTCCAT CTTTTACGTG GTGTACTTCG TGGTCTTCCC CTTCTTCTTC
    GTCAACATCT TCGTGGCTCT TATCATCATT ACTTTCCAGG AGCAAGGAGA CAAAATGATG
    GAGGACTATA GCCTGGAAAA GAATGAGAGA GCCTGCATAG ACTTTGCCAT CAACGCTAAG
    CCGCTCACAC GGCACATGCC CCAGAACAAG CAGACCTTTC AGTACCGCAT GTGGGAGTTT
    GTGGTGTCTC CTCCATTTGA ATACACCATC ATGGCCTTGA TCGCGCTCAA CACTATTGTC
    CTTATGATGA AGTATGATGG AGCATCGCTT ACTTATGAAG ATGTGCTAAA ATACCTGAAC
    ATAGTGTTCA CCTCTCTATT CTCCATGGAG TGCATATTGA AGATCATAGC CTTCGGAGCA
    TTGAACTACT TCAAAGATGC CTGGAACATC TTCGACTGTG TGACTGTACT TGGCAGCATC
    ACTGATATTT TAGTCACAGA GCTGGGGAAC AACTTCATAA ATCTGAGTTT TCTAAGGCTC
    TTCAGAGCTG CTCGTCTCAT TAAGTTGCTC AGGCAGGGTG AAACCATACG CATCCTGCTC
    TGGACTTTTG TCCAGTCTTT CAAGGCTCTG CCGTATGTGT GCCTACTCAT CGCCATGCTG
    TTCTTCATCT ACGCCATCAT TGGCATGCAG CTGTTTGGGA ACATTAAGAT CGAGGAGAAC
    AGTGACAGTG CCATTACCCA GCACAACAAC TTCAGGACTT TTTTCCAGGC CCTAATGCTG
    CTCTTTAGGA GTGCTACAGG TGAGGCATGG CATGACATCA TGCTGTCATG TCTGGGAAAG
    AAGCCGTGTG ATATTCTCTC TGATAACCCG AAGCCAGAAT GCGGGAGCGA GTTTGCCTAC
    CTCTACTTTG TCTCCTTCAT CTTCCTCTGT TCATTTCTGA TGCTGAATCT GTTCGTGGCT
    GTGATCATGG ATAATTTTGA ATACCTGACG CGTGACTCCT CTATCCTGGG ACCCCATCAC
    CTGGACGAGT ATGTGAGGAT CTGGGCAGAA TATGATCCTG CTGCTTGTGG CAGAATCAGT
    TACAGGGATA TGTATGAGAT GCTCAGGCAC ATGTCTCCTC CGTTAGGCCT GGGGAAGAAG
    TGCCCTGCAC GGGTTGCCTA CAAGAGACTG TTGCGGATGG ATCTGCCGGT TGCCGATGAC
    AACACAGTGC ACTTTAATTC TACCCTTATG GCCTTAATCC GAACCGCTCT GGACATCAAG
    ATCGCCAAGG GAGGTGTAGA TAAGCACCAG ATGGATGCTG AATTAAGGAA GGAGATGATG
    GCGATATGGC CTAATCTCTC TCAGAAGAAC CTGGACCTGC TGGTGACTCC TCACAAGTCG
    ACAGACCTGA CAGTAGGAAA GATCTACGCT GCCATGATGA TCATGGAGTA TTATCGGCAA
    AGTAAAGCCA AACGGACACA AGCTCTCCAT GATGAGCAGA ATCGAACGCC ATTAATGTTT
    CAGCGTCTGG AGCCGCCATC TCCTAGCCAG GATGTGGGAC CAGGACTCAC TGGCCTTCCT
    GACACACAAA TGCACCCTGC AAATCATCTG CCTGTTGATG AAAGGATCCC AGAGAGCCAA
    TCATGGGTGA CAGCGCGAGC ACAGGAGATT TCTCAAAAAG CAGGCAACTG GAGTCCAGAG
    GGCCAACATC CAGATGACAC CACAGACAAC AGAAGGCAGT CTCAGACAGT AGAGATGAGA
    GAGATGGGGC GGGACGGGTA CTCGGACACA GAGCAATATC TGCCGATGGA AGGCCATGGC
    AGGGCTGCTT CCATGCCTCG ACTCCCAGCA GACAACCAAC AGCCACGGAG GAAAGGGCGT
    CACCGGGGAG AAAATCTCAC TCAGCCTATC ACAGATAACA GTCCTATGAA GCGCTCGGCT
    TCATCTTTGG GACACGGGCG TGCAGGACGC TCCATGCGAG GTGAGGATTA TGCCATGGAG
    AGGGTTATCC CAGAAGAGGG TCACAGGCAT GGGCATCGCC ACCGAGACCG TAGTCACCGG
    GCGTCAGAAC GCTCCCTGAG CCGATACACT GATGCTGACA CAGGTCTTGG AACTGACCTC
    AGCACAACCA CACAATCAGG AGACCTGCCA TCCAAGGAGA GAGACCGGGG CCGAGCCAAA
    GACCGCAAGC ACCATCATCA TCATCATCAC CACCACGGCT CTTTAGATAA GGAGCACTAC
    GGTCATGAGA GAGACAGAGG AGAGTACGGC CACAGACAGT CCAGAGAGAG AGACCGCCGC
    TGGTCCCGCT CACCCAGTGA GGGCAGAGAG TGTCTGACTC ACAGACAGGG CAGTAGTTCA
    GTAAGCGGCA GTCCAGTCCC GTCCACCTCC GGGACCAGCA CCCCCCGCCG TGGCCGCCGT
    CAGCTCCCCC AAACCCCTGC TACACCCCGG CCACATGTCA CCTACTCACC TGTGGTCCGT
    AAGCCCATCA GCAGTACCCC CCCTCCTGGC CAGCAGAGCC GACTCCCTAC CCCGACCTCC
    CGGCGCTTCA GTCCGACTGG CCCCGAGCCC CCGCTTCCCC CTCATCACCA CCCGTCACCA
    CCCCACCACG GCTCTCCCCG CTCCGGCCGA CATGCACACT GGGGCCCCGA GCCTGCAGAG
    AGCGTGGAGG GTGACGGCTT CTATGACGAC CAAGACTATG AGTTCAATCA CCACGAGCCT
    CCATCCTACG AGCAAAGCCC TGTGCAGGGC AACCCTCACC CCCATTCCCC TAGGACTTCC
    CGCCACAACA CCCCTCCTCA GGGCCAAGCG CACCCACGGC GCATGCCAAA TGGCTACCGT
    TCGTCTTCGC CCTCTCCACA CCACCACGCG CCCGCTCACC CTGGAACTCA TAAACCTCCT
    CATCCCAGAG GGCCCAGAAA GGGCCTCCAT GAACCCTACA GTGAGACTGA CGAGGATGAC
    TGGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330087.1
    CDS1509..8597
    Translation

    Target ORF information:

    RefSeq Version XM_021474412.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474412.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACAGGAAGC AGCAAAGGGA AAGACATCAG TACTATCAAG
    TCTCTACGTG TGCTTCGAGT ACTGCGGCCG CTGAAAACCA TCAAACGTCT GCCAAAACTG
    AAGGCTGTCT TTGACTGTGT GGTGAACTCT CTGAAGAATG TGTTGAACAT CCTCATCGTC
    TACATGCTCT TCATGTTCAT CTTCGCTGTG GTGGCTGTGC AGCTCTTTAA GGGACGTTTC
    TTTTACTGCA CTGATGAATC CAAAGAGTTT GAGCGCGACT GCAGGGGCGA GTATCTGGTC
    TATGAAAGAG ATAACGAAGT GAGGTCACAG AAGCGGGAAT GGAAGAAATA CGATTTCCAC
    TACGACAACG TGCTCTGGGC CCTTCTCACC CTCTTCACCG TTTCTACGGG AGAGGGGTGG
    CCGCAAGTGC TAAAACATTC AGTAGATGCG ACCTACGAGA ATCAGGGCCC GAGCCCAGGT
    TACCGGATGG AAATGTCCAT CTTTTACGTG GTGTACTTCG TGGTCTTCCC CTTCTTCTTC
    GTCAACATCT TCGTGGCTCT TATCATCATT ACTTTCCAGG AGCAAGGAGA CAAAATGATG
    GAGGACTATA GCCTGGAAAA GAATGAGAGA GCCTGCATAG ACTTTGCCAT CAACGCTAAG
    CCGCTCACAC GGCACATGCC CCAGAACAAG CAGACCTTTC AGTACCGCAT GTGGGAGTTT
    GTGGTGTCTC CTCCATTTGA ATACACCATC ATGGCCTTGA TCGCGCTCAA CACTATTGTC
    CTTATGATGA AGTATGATGG AGCATCGCTT ACTTATGAAG ATGTGCTAAA ATACCTGAAC
    ATAGTGTTCA CCTCTCTATT CTCCATGGAG TGCATATTGA AGATCATAGC CTTCGGAGCA
    TTGAACTACT TCAAAGATGC CTGGAACATC TTCGACTGTG TGACTGTACT TGGCAGCATC
    ACTGATATTT TAGTCACAGA GCTGGGGAAC AACTTCATAA ATCTGAGTTT TCTAAGGCTC
    TTCAGAGCTG CTCGTCTCAT TAAGTTGCTC AGGCAGGGTG AAACCATACG CATCCTGCTC
    TGGACTTTTG TCCAGTCTTT CAAGGCTCTG CCGTATGTGT GCCTACTCAT CGCCATGCTG
    TTCTTCATCT ACGCCATCAT TGGCATGCAG CTGTTTGGGA ACATTAAGAT CGAGGAGAAC
    AGTGACAGTG CCATTACCCA GCACAACAAC TTCAGGACTT TTTTCCAGGC CCTAATGCTG
    CTCTTTAGGA GTGCTACAGG TGAGGCATGG CATGACATCA TGCTGTCATG TCTGGGAAAG
    AAGCCGTGTG ATATTCTCTC TGATAACCCG AAGCCAGAAT GCGGGAGCGA GTTTGCCTAC
    CTCTACTTTG TCTCCTTCAT CTTCCTCTGT TCATTTCTGA TGCTGAATCT GTTCGTGGCT
    GTGATCATGG ATAATTTTGA ATACCTGACG CGTGACTCCT CTATCCTGGG ACCCCATCAC
    CTGGACGAGT ATGTGAGGAT CTGGGCAGAA TATGATCCTG CTGCTTGTGG CAGAATCAGT
    TACAGGGATA TGTATGAGAT GCTCAGGCAC ATGTCTCCTC CGTTAGGCCT GGGGAAGAAG
    TGCCCTGCAC GGGTTGCCTA CAAGAGACTG TTGCGGATGG ATCTGCCGGT TGCCGATGAC
    AACACAGTGC ACTTTAATTC TACCCTTATG GCCTTAATCC GAACCGCTCT GGACATCAAG
    ATCGCCAAGG GAGGTGTAGA TAAGCACCAG ATGGATGCTG AATTAAGGAA GGAGATGATG
    GCGATATGGC CTAATCTCTC TCAGAAGAAC CTGGACCTGC TGGTGACTCC TCACAAGTCG
    ACAGACCTGA CAGTAGGAAA GATCTACGCT GCCATGATGA TCATGGAGTA TTATCGGCAA
    AGTAAAGCCA AACGGACACA AGCTCTCCAT GATGAGCAGA ATCGAACGCC ATTAATGTTT
    CAGCGTCTGG AGCCGCCATC TCCTAGCCAG GATGTGGGAC CAGGACTCAC TGGCCTTCCT
    GACACACAAA TGCACCCTGC AAATCATCTG CCTGTTGATG AAAGGATCCC AGAGAGCCAA
    TCATGGGTGA CAGCGCGAGC ACAGGAGATT TCTCAAAAAG CAGGCAACTG GAGTCCAGAG
    GGCCAACATC CAGATGACAC CACAGACAAC AGAAGGCAGT CTCAGACAGT AGAGATGAGA
    GAGATGGGGC GGGACGGGTA CTCGGACACA GAGCAATATC TGCCGATGGA AGGCCATGGC
    AGGGCTGCTT CCATGCCTCG ACTCCCAGCA GACAACCAAC AGCCACGGAG GAAAGGGCGT
    CACCGGGGAG AAAATCTCAC TCAGCCTATC ACAGATAACA GTCCTATGAA GCGCTCGGCT
    TCATCTTTGG GACACGGGCG TGCAGGACGC TCCATGCGAG GTGAGGATTA TGCCATGGAG
    AGGGTTATCC CAGAAGAGGG TCACAGGCAT GGGCATCGCC ACCGAGACCG TAGTCACCGG
    GCGTCAGAAC GCTCCCTGAG CCGATACACT GATGCTGACA CAGGTCTTGG AACTGACCTC
    AGCACAACCA CACAATCAGG AGACCTGCCA TCCAAGGAGA GAGACCGGGG CCGAGCCAAA
    GACCGCAAGC ACCATCATCA TCATCATCAC CACCACGGCT CTTTAGATAA GGAGCACTAC
    GGTCATGAGA GAGACAGAGG AGAGTACGGC CACAGACAGT CCAGAGAGAG AGACCGCCGC
    TGGTCCCGCT CACCCAGTGA GGGCAGAGAG TGTCTGACTC ACAGACAGGG CAGTAGTTCA
    GTAAGCGGCA GTCCAGTCCC GTCCACCTCC GGGACCAGCA CCCCCCGCCG TGGCCGCCGT
    CAGCTCCCCC AAACCCCTGC TACACCCCGG CCACATGTCA CCTACTCACC TGTGGTCCGT
    AAGCCCATCA GCAGTACCCC CCCTCCTGGC CAGCAGAGCC GACTCCCTAC CCCGACCTCC
    CGGCGCTTCA GTCCGACTGG CCCCGAGCCC CCGCTTCCCC CTCATCACCA CCCGTCACCA
    CCCCACCACG GCTCTCCCCG CTCCGGCCGA CATGCACACT GGGGCCCCGA GCCTGCAGAG
    AGCGTGGAGG GTGACGGCTT CTATGACGAC CAAGACTATG AGTTCAATCA CCACGAGCCT
    CCATCCTACG AGCAAAGCCC TGTGCAGGGC AACCCTCACC CCCATTCCCC TAGGACTTCC
    CGCCACAACA CCCCTCCTCA GGGCCAAGCG CACCCACGGC GCATGCCAAA TGGCTACCGT
    TCGTCTTCGC CCTCTCCACA CCACCACGCG CCCGCTCACC CTGGAACTCA TAAACCTCCT
    CATCCCAGAG GGCCCAGAAA GGGCCTCCAT GAACCCTACA GTGAGACTGA CGAGGATGAC
    TGGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141796
    Clone ID Related Accession (Same CDS sequence) XM_021474420.1
    Accession Version XM_021474420.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7065bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCTAAGGA GCAGCAGAAG AACCACAAAG GTTGTAAGTC GGTATGGGAG
    CAGCGCACTA GTGAGTTAAG ACGACAGACC CTCGTGAACA GCAGAGAGGC TCTTTATAAT
    GAGTTGGACC CTGAAGACCG TTGGAAGGTC TCATACTCCC GTCACATACG TCCCGACATG
    AAAACTCACC TGGACCGGCC GTTAGTCGTG GACCCTCAGG AGAACCGCAA TAACAACACT
    AACAAAACCC GTCCGGGTGA CGGCCAACCT CAGCATTCCC ATCAGCTCCT ACACAAACAG
    CCCAACTTCA ATGAGGCCGA GACTGGAGGA GGTGGAGGGT CTGAGTCCTG GCCCGCACTG
    GAAAGAGGAG ACTCAACAGG GTCCCGCCGC AGTCTTTTGG GCTCTGAGCA CTATGAGGAG
    GGTAGAGAGT CCCACCGCAG ACACCAGCAC AGCCAGCACT GCAGTCAGGC CCGTCAACAT
    CGCAAAGCTG CAGAGGGCGG TGGAGAGCCT GGAGGGAGGA GACACAGGAG TAAGGCCAAG
    GAAAGAGGAC CTGAGTGTGA GCATTCAGAT GGAGGGGAGC GGAAACACAG ACGGCATCGG
    CATGAGGGTG AGGGGAGGAA AGAGAGAGGG GTACGACACC GAAACAGGAG GGATGGCCAT
    GCAAGTGGCC CGACTCTCTC TACAACCCGT CCCATTCAGA AGACTCTGTC CAGGCAGGAC
    AGTCAGTACA GCGAAGACCT GGACAATGCC ATGAACAACA AACTTGCTAC CCAGCCACAT
    GATAGTCTGC TTAATCTGGC CAACACTGCC CACTCTGCCG GCCTGGCACA TACTGGTACA
    GAGAGCAGCC TCATCCTCAC CAACCCTTCC TCTACCATTG CAAATGTTAC TAGCATCGGT
    CACCTGGGTA TGAAACCTGA GTACACTGCC GTGGACATTC CTCCCATGTT CCCCTCCAGT
    AATGCCATTC TACAGGTCAA TAAGAATGCC AACACGGAGC CTCTGCCAAA GAAGGAAGAC
    ACAAAGGGTG ACGATGATGA TGATGAGAAA GATGACGGAG GGCCTAAACC CATGCCACCC
    TACACCTCAA TGTTCATTCT GACCACCACC AACCCATTTC GGAGATTGTG TCACTATATC
    GTCACACTGA GATATTTTGA GATGTGTATT CTACTGGTTA TTGCTATGAG CAGTATTGCC
    CTGGCTGCTG AAGACCCTGT CTGGCCTGAA TCTCCCCGCA ACAATGTCCT ACGATACTTT
    GACTATGTGT TCACTGGTGT GTTCACCTTT GAGATGCTCA TTAAGATGGT AGATTTAGGT
    CTTGTCCTGC ATCAAGGCTC ATACTTTCGT GATCTGTGGA ACATCCTGGA CTTTATAGTG
    GTTAGTGGTG CCCTGGTGGC GTTCGCCTTC ACCGGAGGCA GCGGAGGAAG CAGCAAAGGG
    AAAGACATCA GTACTATCAA GTCTCTACGT GTGCTTCGAG TACTGCGGCC GCTGAAAACC
    ATCAAACGTC TGCCAAAACT GAAGGCTGTC TTTGACTGTG TGGTGAACTC TCTGAAGAAT
    GTGTTGAACA TCCTCATCGT CTACATGCTC TTCATGTTCA TCTTCGCTGT GGTGGCTGTG
    CAGCTCTTTA AGGGACGTTT CTTTTACTGC ACTGATGAAT CCAAAGAGTT TGAGCGCGAC
    TGCAGGGGCG AGTATCTGGT CTATGAAAGA GATAACGAAG TGAGGTCACA GAAGCGGGAA
    TGGAAGAAAT ACGATTTCCA CTACGACAAC GTGCTCTGGG CCCTTCTCAC CCTCTTCACC
    GTTTCTACGG GAGAGGGGTG GCCGCAAGTG CTAAAACATT CAGTAGATGC GACCTACGAG
    AATCAGGGCC CGAGCCCAGG TTACCGGATG GAAATGTCCA TCTTTTACGT GGTGTACTTC
    GTGGTCTTCC CCTTCTTCTT CGTCAACATC TTCGTGGCTC TTATCATCAT TACTTTCCAG
    GAGCAAGGAG ACAAAATGAT GGAGGACTAT AGCCTGGAAA AGAATGAGAG AGCCTGCATA
    GACTTTGCCA TCAACGCTAA GCCGCTCACA CGGCACATGC CCCAGAACAA GCAGACCTTT
    CAGTACCGCA TGTGGGAGTT TGTGGTGTCT CCTCCATTTG AATACACCAT CATGGCCTTG
    ATCGCGCTCA ACACTATTGT CCTTATGATG AAGTATGATG GAGCATCGCT TACTTATGAA
    GATGTGCTAA AATACCTGAA CATAGTGTTC ACCTCTCTAT TCTCCATGGA GTGCATATTG
    AAGATCATAG CCTTCGGAGC ATTGAACTAC TTCAAAGATG CCTGGAACAT CTTCGACTGT
    GTGACTGTAC TTGGCAGCAT CACTGATATT TTAGTCACAG AGCTGGGGAT GAACAACTTC
    ATAAATCTGA GTTTTCTAAG GCTCTTCAGA GCTGCTCGTC TCATTAAGTT GCTCAGGCAG
    GGTGAAACCA TACGCATCCT GCTCTGGACT TTTGTCCAGT CTTTCAAGGC TCTGCCGTAT
    GTGTGCCTAC TCATCGCCAT GCTGTTCTTC ATCTACGCCA TCATTGGCAT GCAGCTGTTT
    GGGAACATTA AGATCGAGGA GAACAGTGAC AGTGCCATTA CCCAGCACAA CAACTTCAGG
    ACTTTTTTCC AGGCCCTAAT GCTGCTCTTT AGGAGTGCTA CAGGTGAGGC ATGGCATGAC
    ATCATGCTGT CATGTCTGGG AAAGAAGCCG TGTGATATTC TCTCTGATAA CCCGAAGCCA
    GAATGCGGGA GCGAGTTTGC CTACCTCTAC TTTGTCTCCT TCATCTTCCT CTGTTCATTT
    CTGATGCTGA ATCTGTTCGT GGCTGTGATC ATGGATAATT TTGAATACCT GACGCGTGAC
    TCCTCTATCC TGGGACCCCA TCACCTGGAC GAGTATGTGA GGATCTGGGC AGAATATGAT
    CCTGCTGCTT GCGGGCGCAT TCATTATAAG GATATGTACA GTTTATTGCG AGTTATCGAC
    CCCCCTCTGG GCTTAGGCAA GAAATGTCCT CATAGGGTGG CCTGCAAGAG ACTGTTGCGG
    ATGGATCTGC CGGTTGCCGA TGACAACACA GTGCACTTTA ATTCTACCCT TATGGCCTTA
    ATCCGAACCG CTCTGGACAT CAAGATCGCC AAGGGTGGGG AAGGAGGTGT AGATAAGCAC
    CAGATGGATG CTGAATTAAG GAAGGAGATG ATGGCGATAT GGCCTAATCT CTCTCAGAAG
    AACCTGGACC TGCTGGTGAC TCCTCACAAG TCAGCGACAG ACCTGACAGT AGGAAAGATC
    TACGCTGCCA TGATGATCAT GGAGTATTAT CGGCAAAGTA AAGCCAAACG GACACAAGCT
    CTCCATGATG AGCAGAATCG AACGCCATTA ATGTTTCAGC GTCTGGAGCC GCCATCTCCT
    AGCCAGGATG TGGGACCAGG ACTCACTGGC CTTCCTGACA CACAAATGCA CCCTGCAAAT
    CATCTGCCTG TTGATGAAAG GATCCCAGAG AGCCAATCAT GGGTGACAGC GCGAGCACAG
    GAGATTTCTC AAAAAGCAGG CAACTGGAGT CCAGAGGGCC AACATCCAGA TGACACCACA
    GACAACAGAA GGCAGTCTCA GACAGTAGAG ATGAGAGAGA TGGGGCGGGA CGGGTACTCG
    GACACAGAGC AATATCTGCC GATGGAAGGC CATGGCAGGG CTGCTTCCAT GCCTCGACTC
    CCAGCAGACA ACCAACAGCC ACGGAGGAAA GGGCGTCACC GGGGAGAAAA TCTCACTCAG
    CCTATCACAG ATAACAGTCC TATGAAGCGC TCGGCTTCAT CTTTGGGACA CGGGCGTGCA
    GGACGCTCCA TGCGAGGTGA GGATTATGCC ATGGAGAGGG TTATCCCAGA AGAGGGTCAC
    AGGCATGGGC ATCGCCACCG AGACCGTAGT CACCGGGCGT CAGAACGCTC CCTGAGCCGA
    TACACTGATG CTGACACAGG TCTTGGAACT GACCTCAGCA CAACCACACA ATCAGGAGAC
    CTGCCATCCA AGGAGAGAGA CCGGGGCCGA GCCAAAGACC GCAAGCACCA TCATCATCAT
    CATCACCACC ACGGCTCTTT AGATAAGGAG CACTACGGTC ATGAGAGAGA CAGAGGAGAG
    TACGGCCACA GACAGTCCAG AGAGAGAGAC CGCCGCTGGT CCCGCTCACC CAGTGAGGGC
    AGAGAGTGTC TGACTCACAG ACAGGGCAGT AGTTCAGTAA GCGGCAGTCC AGTCCCGTCC
    ACCTCCGGGA CCAGCACCCC CCGCCGTGGC CGCCGTCAGC TCCCCCAAAC CCCTGCTACA
    CCCCGGCCAC ATGTCACCTA CTCACCTGTG GTCCGTAAGC CCATCAGCAG TACCCCCCCT
    CCTGGCCAGC AGAGCCGACT CCCTACCCCG ACCTCCCGGC GCTTCAGTCC GACTGGCCCC
    GAGCCCCCGC TTCCCCCTCA TCACCACCCG TCACCACCCC ACCACGGCTC TCCCCGCTCC
    GGCCGACATG CACACTGGGG CCCCGAGCCT GCAGAGAGCG TGGAGGGTGA CGGCTTCTAT
    GACGACCAAG ACTATGAGTT CAATCACCAC GAGCCTCCAT CCTACGAGCA AAGCCCTGTG
    CAGGGCAACC CTCACCCCCA TTCCCCTAGG ACTTCCCGCC ACAACACCCC TCCTCAGGGC
    CAAGCGCACC CACGGCGCAT GCCAAATGGC TACCGTTCGT CTTCGCCCTC TCCACACCAC
    CACGCGCCCG CTCACCCTGG AACTCATAAA CCTCCTCATC CCAGAGGGCC CAGAAAGGGC
    CTCCATGAAC CCTACAGTGA GACTGACGAG GATGACTGGT GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330095.1
    CDS1506..8570
    Translation

    Target ORF information:

    RefSeq Version XM_021474420.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474420.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCTAAGGA GCAGCAGAAG AACCACAAAG GTTGTAAGTC GGTATGGGAG
    CAGCGCACTA GTGAGTTAAG ACGACAGACC CTCGTGAACA GCAGAGAGGC TCTTTATAAT
    GAGTTGGACC CTGAAGACCG TTGGAAGGTC TCATACTCCC GTCACATACG TCCCGACATG
    AAAACTCACC TGGACCGGCC GTTAGTCGTG GACCCTCAGG AGAACCGCAA TAACAACACT
    AACAAAACCC GTCCGGGTGA CGGCCAACCT CAGCATTCCC ATCAGCTCCT ACACAAACAG
    CCCAACTTCA ATGAGGCCGA GACTGGAGGA GGTGGAGGGT CTGAGTCCTG GCCCGCACTG
    GAAAGAGGAG ACTCAACAGG GTCCCGCCGC AGTCTTTTGG GCTCTGAGCA CTATGAGGAG
    GGTAGAGAGT CCCACCGCAG ACACCAGCAC AGCCAGCACT GCAGTCAGGC CCGTCAACAT
    CGCAAAGCTG CAGAGGGCGG TGGAGAGCCT GGAGGGAGGA GACACAGGAG TAAGGCCAAG
    GAAAGAGGAC CTGAGTGTGA GCATTCAGAT GGAGGGGAGC GGAAACACAG ACGGCATCGG
    CATGAGGGTG AGGGGAGGAA AGAGAGAGGG GTACGACACC GAAACAGGAG GGATGGCCAT
    GCAAGTGGCC CGACTCTCTC TACAACCCGT CCCATTCAGA AGACTCTGTC CAGGCAGGAC
    AGTCAGTACA GCGAAGACCT GGACAATGCC ATGAACAACA AACTTGCTAC CCAGCCACAT
    GATAGTCTGC TTAATCTGGC CAACACTGCC CACTCTGCCG GCCTGGCACA TACTGGTACA
    GAGAGCAGCC TCATCCTCAC CAACCCTTCC TCTACCATTG CAAATGTTAC TAGCATCGGT
    CACCTGGGTA TGAAACCTGA GTACACTGCC GTGGACATTC CTCCCATGTT CCCCTCCAGT
    AATGCCATTC TACAGGTCAA TAAGAATGCC AACACGGAGC CTCTGCCAAA GAAGGAAGAC
    ACAAAGGGTG ACGATGATGA TGATGAGAAA GATGACGGAG GGCCTAAACC CATGCCACCC
    TACACCTCAA TGTTCATTCT GACCACCACC AACCCATTTC GGAGATTGTG TCACTATATC
    GTCACACTGA GATATTTTGA GATGTGTATT CTACTGGTTA TTGCTATGAG CAGTATTGCC
    CTGGCTGCTG AAGACCCTGT CTGGCCTGAA TCTCCCCGCA ACAATGTCCT ACGATACTTT
    GACTATGTGT TCACTGGTGT GTTCACCTTT GAGATGCTCA TTAAGATGGT AGATTTAGGT
    CTTGTCCTGC ATCAAGGCTC ATACTTTCGT GATCTGTGGA ACATCCTGGA CTTTATAGTG
    GTTAGTGGTG CCCTGGTGGC GTTCGCCTTC ACCGGAGGCA GCGGAGGAAG CAGCAAAGGG
    AAAGACATCA GTACTATCAA GTCTCTACGT GTGCTTCGAG TACTGCGGCC GCTGAAAACC
    ATCAAACGTC TGCCAAAACT GAAGGCTGTC TTTGACTGTG TGGTGAACTC TCTGAAGAAT
    GTGTTGAACA TCCTCATCGT CTACATGCTC TTCATGTTCA TCTTCGCTGT GGTGGCTGTG
    CAGCTCTTTA AGGGACGTTT CTTTTACTGC ACTGATGAAT CCAAAGAGTT TGAGCGCGAC
    TGCAGGGGCG AGTATCTGGT CTATGAAAGA GATAACGAAG TGAGGTCACA GAAGCGGGAA
    TGGAAGAAAT ACGATTTCCA CTACGACAAC GTGCTCTGGG CCCTTCTCAC CCTCTTCACC
    GTTTCTACGG GAGAGGGGTG GCCGCAAGTG CTAAAACATT CAGTAGATGC GACCTACGAG
    AATCAGGGCC CGAGCCCAGG TTACCGGATG GAAATGTCCA TCTTTTACGT GGTGTACTTC
    GTGGTCTTCC CCTTCTTCTT CGTCAACATC TTCGTGGCTC TTATCATCAT TACTTTCCAG
    GAGCAAGGAG ACAAAATGAT GGAGGACTAT AGCCTGGAAA AGAATGAGAG AGCCTGCATA
    GACTTTGCCA TCAACGCTAA GCCGCTCACA CGGCACATGC CCCAGAACAA GCAGACCTTT
    CAGTACCGCA TGTGGGAGTT TGTGGTGTCT CCTCCATTTG AATACACCAT CATGGCCTTG
    ATCGCGCTCA ACACTATTGT CCTTATGATG AAGTATGATG GAGCATCGCT TACTTATGAA
    GATGTGCTAA AATACCTGAA CATAGTGTTC ACCTCTCTAT TCTCCATGGA GTGCATATTG
    AAGATCATAG CCTTCGGAGC ATTGAACTAC TTCAAAGATG CCTGGAACAT CTTCGACTGT
    GTGACTGTAC TTGGCAGCAT CACTGATATT TTAGTCACAG AGCTGGGGAT GAACAACTTC
    ATAAATCTGA GTTTTCTAAG GCTCTTCAGA GCTGCTCGTC TCATTAAGTT GCTCAGGCAG
    GGTGAAACCA TACGCATCCT GCTCTGGACT TTTGTCCAGT CTTTCAAGGC TCTGCCGTAT
    GTGTGCCTAC TCATCGCCAT GCTGTTCTTC ATCTACGCCA TCATTGGCAT GCAGCTGTTT
    GGGAACATTA AGATCGAGGA GAACAGTGAC AGTGCCATTA CCCAGCACAA CAACTTCAGG
    ACTTTTTTCC AGGCCCTAAT GCTGCTCTTT AGGAGTGCTA CAGGTGAGGC ATGGCATGAC
    ATCATGCTGT CATGTCTGGG AAAGAAGCCG TGTGATATTC TCTCTGATAA CCCGAAGCCA
    GAATGCGGGA GCGAGTTTGC CTACCTCTAC TTTGTCTCCT TCATCTTCCT CTGTTCATTT
    CTGATGCTGA ATCTGTTCGT GGCTGTGATC ATGGATAATT TTGAATACCT GACGCGTGAC
    TCCTCTATCC TGGGACCCCA TCACCTGGAC GAGTATGTGA GGATCTGGGC AGAATATGAT
    CCTGCTGCTT GCGGGCGCAT TCATTATAAG GATATGTACA GTTTATTGCG AGTTATCGAC
    CCCCCTCTGG GCTTAGGCAA GAAATGTCCT CATAGGGTGG CCTGCAAGAG ACTGTTGCGG
    ATGGATCTGC CGGTTGCCGA TGACAACACA GTGCACTTTA ATTCTACCCT TATGGCCTTA
    ATCCGAACCG CTCTGGACAT CAAGATCGCC AAGGGTGGGG AAGGAGGTGT AGATAAGCAC
    CAGATGGATG CTGAATTAAG GAAGGAGATG ATGGCGATAT GGCCTAATCT CTCTCAGAAG
    AACCTGGACC TGCTGGTGAC TCCTCACAAG TCAGCGACAG ACCTGACAGT AGGAAAGATC
    TACGCTGCCA TGATGATCAT GGAGTATTAT CGGCAAAGTA AAGCCAAACG GACACAAGCT
    CTCCATGATG AGCAGAATCG AACGCCATTA ATGTTTCAGC GTCTGGAGCC GCCATCTCCT
    AGCCAGGATG TGGGACCAGG ACTCACTGGC CTTCCTGACA CACAAATGCA CCCTGCAAAT
    CATCTGCCTG TTGATGAAAG GATCCCAGAG AGCCAATCAT GGGTGACAGC GCGAGCACAG
    GAGATTTCTC AAAAAGCAGG CAACTGGAGT CCAGAGGGCC AACATCCAGA TGACACCACA
    GACAACAGAA GGCAGTCTCA GACAGTAGAG ATGAGAGAGA TGGGGCGGGA CGGGTACTCG
    GACACAGAGC AATATCTGCC GATGGAAGGC CATGGCAGGG CTGCTTCCAT GCCTCGACTC
    CCAGCAGACA ACCAACAGCC ACGGAGGAAA GGGCGTCACC GGGGAGAAAA TCTCACTCAG
    CCTATCACAG ATAACAGTCC TATGAAGCGC TCGGCTTCAT CTTTGGGACA CGGGCGTGCA
    GGACGCTCCA TGCGAGGTGA GGATTATGCC ATGGAGAGGG TTATCCCAGA AGAGGGTCAC
    AGGCATGGGC ATCGCCACCG AGACCGTAGT CACCGGGCGT CAGAACGCTC CCTGAGCCGA
    TACACTGATG CTGACACAGG TCTTGGAACT GACCTCAGCA CAACCACACA ATCAGGAGAC
    CTGCCATCCA AGGAGAGAGA CCGGGGCCGA GCCAAAGACC GCAAGCACCA TCATCATCAT
    CATCACCACC ACGGCTCTTT AGATAAGGAG CACTACGGTC ATGAGAGAGA CAGAGGAGAG
    TACGGCCACA GACAGTCCAG AGAGAGAGAC CGCCGCTGGT CCCGCTCACC CAGTGAGGGC
    AGAGAGTGTC TGACTCACAG ACAGGGCAGT AGTTCAGTAA GCGGCAGTCC AGTCCCGTCC
    ACCTCCGGGA CCAGCACCCC CCGCCGTGGC CGCCGTCAGC TCCCCCAAAC CCCTGCTACA
    CCCCGGCCAC ATGTCACCTA CTCACCTGTG GTCCGTAAGC CCATCAGCAG TACCCCCCCT
    CCTGGCCAGC AGAGCCGACT CCCTACCCCG ACCTCCCGGC GCTTCAGTCC GACTGGCCCC
    GAGCCCCCGC TTCCCCCTCA TCACCACCCG TCACCACCCC ACCACGGCTC TCCCCGCTCC
    GGCCGACATG CACACTGGGG CCCCGAGCCT GCAGAGAGCG TGGAGGGTGA CGGCTTCTAT
    GACGACCAAG ACTATGAGTT CAATCACCAC GAGCCTCCAT CCTACGAGCA AAGCCCTGTG
    CAGGGCAACC CTCACCCCCA TTCCCCTAGG ACTTCCCGCC ACAACACCCC TCCTCAGGGC
    CAAGCGCACC CACGGCGCAT GCCAAATGGC TACCGTTCGT CTTCGCCCTC TCCACACCAC
    CACGCGCCCG CTCACCCTGG AACTCATAAA CCTCCTCATC CCAGAGGGCC CAGAAAGGGC
    CTCCATGAAC CCTACAGTGA GACTGACGAG GATGACTGGT GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141797
    Clone ID Related Accession (Same CDS sequence) XM_021474422.1
    Accession Version XM_021474422.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7002bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG
    GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT
    GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC
    CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC
    ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT
    GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT
    GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT
    GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT
    CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA
    AGGCAGTCTC AGCAGCCACG GAGGAAAGGG CGTCACCGGG GAGAAAATCT CACTCAGCCT
    ATCACAGATA ACAGTCCTAT GAAGCGCTCG GCTTCATCTT TGGGACACGG GCGTGCAGGA
    CGCTCCATGC GAGGTGAGGA TTATGCCATG GAGAGGGTTA TCCCAGAAGA GGGTCACAGG
    CATGGGCATC GCCACCGAGA CCGTAGTCAC CGGGCGTCAG AACGCTCCCT GAGCCGATAC
    ACTGATGCTG ACACAGGTCT TGGAACTGAC CTCAGCACAA CCACACAATC AGGAGACCTG
    CCATCCAAGG AGAGAGACCG GGGCCGAGCC AAAGACCGCA AGCACCATCA TCATCATCAT
    CACCACCACG GCTCTTTAGA TAAGGAGCAC TACGGTCATG AGAGAGACAG AGGAGAGTAC
    GGCCACAGAC AGTCCAGAGA GAGAGACCGC CGCTGGTCCC GCTCACCCAG TGAGGGCAGA
    GAGTGTCTGA CTCACAGACA GGGCAGTAGT TCAGTAAGCG GCAGTCCAGT CCCGTCCACC
    TCCGGGACCA GCACCCCCCG CCGTGGCCGC CGTCAGCTCC CCCAAACCCC TGCTACACCC
    CGGCCACATG TCACCTACTC ACCTGTGGTC CGTAAGCCCA TCAGCAGTAC CCCCCCTCCT
    GGCCAGCAGA GCCGACTCCC TACCCCGACC TCCCGGCGCT TCAGTCCGAC TGGCCCCGAG
    CCCCCGCTTC CCCCTCATCA CCACCCGTCA CCACCCCACC ACGGCTCTCC CCGCTCCGGC
    CGACATGCAC ACTGGGGCCC CGAGCCTGCA GAGAGCGTGG AGGGTGACGG CTTCTATGAC
    GACCAAGACT ATGAGTTCAA TCACCACGAG CCTCCATCCT ACGAGCAAAG CCCTGTGCAG
    GGCAACCCTC ACCCCCATTC CCCTAGGACT TCCCGCCACA ACACCCCTCC TCAGGGCCAA
    GCGCACCCAC GGCGCATGCC AAATGGCTAC CGTTCGTCTT CGCCCTCTCC ACACCACCAC
    GCGCCCGCTC ACCCTGGAAC TCATAAACCT CCTCATCCCA GAGGGCCCAG AAAGGGCCTC
    CATGAACCCT ACAGTGAGAC TGACGAGGAT GACTGGTGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330097.1
    CDS1509..8510
    Translation

    Target ORF information:

    RefSeq Version XM_021474422.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474422.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG
    GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT
    GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC
    CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC
    ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT
    GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT
    GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT
    GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT
    CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA
    AGGCAGTCTC AGCAGCCACG GAGGAAAGGG CGTCACCGGG GAGAAAATCT CACTCAGCCT
    ATCACAGATA ACAGTCCTAT GAAGCGCTCG GCTTCATCTT TGGGACACGG GCGTGCAGGA
    CGCTCCATGC GAGGTGAGGA TTATGCCATG GAGAGGGTTA TCCCAGAAGA GGGTCACAGG
    CATGGGCATC GCCACCGAGA CCGTAGTCAC CGGGCGTCAG AACGCTCCCT GAGCCGATAC
    ACTGATGCTG ACACAGGTCT TGGAACTGAC CTCAGCACAA CCACACAATC AGGAGACCTG
    CCATCCAAGG AGAGAGACCG GGGCCGAGCC AAAGACCGCA AGCACCATCA TCATCATCAT
    CACCACCACG GCTCTTTAGA TAAGGAGCAC TACGGTCATG AGAGAGACAG AGGAGAGTAC
    GGCCACAGAC AGTCCAGAGA GAGAGACCGC CGCTGGTCCC GCTCACCCAG TGAGGGCAGA
    GAGTGTCTGA CTCACAGACA GGGCAGTAGT TCAGTAAGCG GCAGTCCAGT CCCGTCCACC
    TCCGGGACCA GCACCCCCCG CCGTGGCCGC CGTCAGCTCC CCCAAACCCC TGCTACACCC
    CGGCCACATG TCACCTACTC ACCTGTGGTC CGTAAGCCCA TCAGCAGTAC CCCCCCTCCT
    GGCCAGCAGA GCCGACTCCC TACCCCGACC TCCCGGCGCT TCAGTCCGAC TGGCCCCGAG
    CCCCCGCTTC CCCCTCATCA CCACCCGTCA CCACCCCACC ACGGCTCTCC CCGCTCCGGC
    CGACATGCAC ACTGGGGCCC CGAGCCTGCA GAGAGCGTGG AGGGTGACGG CTTCTATGAC
    GACCAAGACT ATGAGTTCAA TCACCACGAG CCTCCATCCT ACGAGCAAAG CCCTGTGCAG
    GGCAACCCTC ACCCCCATTC CCCTAGGACT TCCCGCCACA ACACCCCTCC TCAGGGCCAA
    GCGCACCCAC GGCGCATGCC AAATGGCTAC CGTTCGTCTT CGCCCTCTCC ACACCACCAC
    GCGCCCGCTC ACCCTGGAAC TCATAAACCT CCTCATCCCA GAGGGCCCAG AAAGGGCCTC
    CATGAACCCT ACAGTGAGAC TGACGAGGAT GACTGGTGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141798
    Clone ID Related Accession (Same CDS sequence) XM_021474423.1
    Accession Version XM_021474423.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6999bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG
    GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT
    GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC
    CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC
    ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT
    GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT
    GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT
    GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT
    CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA
    AGGCAGTCTC AGCAGCCACG GAGGAAAGGG CGTCACCGGG GAGAAAATCT CACTCCTATC
    ACAGATAACA GTCCTATGAA GCGCTCGGCT TCATCTTTGG GACACGGGCG TGCAGGACGC
    TCCATGCGAG GTGAGGATTA TGCCATGGAG AGGGTTATCC CAGAAGAGGG TCACAGGCAT
    GGGCATCGCC ACCGAGACCG TAGTCACCGG GCGTCAGAAC GCTCCCTGAG CCGATACACT
    GATGCTGACA CAGGTCTTGG AACTGACCTC AGCACAACCA CACAATCAGG AGACCTGCCA
    TCCAAGGAGA GAGACCGGGG CCGAGCCAAA GACCGCAAGC ACCATCATCA TCATCATCAC
    CACCACGGCT CTTTAGATAA GGAGCACTAC GGTCATGAGA GAGACAGAGG AGAGTACGGC
    CACAGACAGT CCAGAGAGAG AGACCGCCGC TGGTCCCGCT CACCCAGTGA GGGCAGAGAG
    TGTCTGACTC ACAGACAGGG CAGTAGTTCA GTAAGCGGCA GTCCAGTCCC GTCCACCTCC
    GGGACCAGCA CCCCCCGCCG TGGCCGCCGT CAGCTCCCCC AAACCCCTGC TACACCCCGG
    CCACATGTCA CCTACTCACC TGTGGTCCGT AAGCCCATCA GCAGTACCCC CCCTCCTGGC
    CAGCAGAGCC GACTCCCTAC CCCGACCTCC CGGCGCTTCA GTCCGACTGG CCCCGAGCCC
    CCGCTTCCCC CTCATCACCA CCCGTCACCA CCCCACCACG GCTCTCCCCG CTCCGGCCGA
    CATGCACACT GGGGCCCCGA GCCTGCAGAG AGCGTGGAGG GTGACGGCTT CTATGACGAC
    CAAGACTATG AGTTCAATCA CCACGAGCCT CCATCCTACG AGCAAAGCCC TGTGCAGGGC
    AACCCTCACC CCCATTCCCC TAGGACTTCC CGCCACAACA CCCCTCCTCA GGGCCAAGCG
    CACCCACGGC GCATGCCAAA TGGCTACCGT TCGTCTTCGC CCTCTCCACA CCACCACGCG
    CCCGCTCACC CTGGAACTCA TAAACCTCCT CATCCCAGAG GGCCCAGAAA GGGCCTCCAT
    GAACCCTACA GTGAGACTGA CGAGGATGAC TGGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330098.1
    CDS1509..8507
    Translation

    Target ORF information:

    RefSeq Version XM_021474423.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474423.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG
    GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT
    GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC
    CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC
    ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT
    GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT
    GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT
    GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT
    CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA
    AGGCAGTCTC AGCAGCCACG GAGGAAAGGG CGTCACCGGG GAGAAAATCT CACTCCTATC
    ACAGATAACA GTCCTATGAA GCGCTCGGCT TCATCTTTGG GACACGGGCG TGCAGGACGC
    TCCATGCGAG GTGAGGATTA TGCCATGGAG AGGGTTATCC CAGAAGAGGG TCACAGGCAT
    GGGCATCGCC ACCGAGACCG TAGTCACCGG GCGTCAGAAC GCTCCCTGAG CCGATACACT
    GATGCTGACA CAGGTCTTGG AACTGACCTC AGCACAACCA CACAATCAGG AGACCTGCCA
    TCCAAGGAGA GAGACCGGGG CCGAGCCAAA GACCGCAAGC ACCATCATCA TCATCATCAC
    CACCACGGCT CTTTAGATAA GGAGCACTAC GGTCATGAGA GAGACAGAGG AGAGTACGGC
    CACAGACAGT CCAGAGAGAG AGACCGCCGC TGGTCCCGCT CACCCAGTGA GGGCAGAGAG
    TGTCTGACTC ACAGACAGGG CAGTAGTTCA GTAAGCGGCA GTCCAGTCCC GTCCACCTCC
    GGGACCAGCA CCCCCCGCCG TGGCCGCCGT CAGCTCCCCC AAACCCCTGC TACACCCCGG
    CCACATGTCA CCTACTCACC TGTGGTCCGT AAGCCCATCA GCAGTACCCC CCCTCCTGGC
    CAGCAGAGCC GACTCCCTAC CCCGACCTCC CGGCGCTTCA GTCCGACTGG CCCCGAGCCC
    CCGCTTCCCC CTCATCACCA CCCGTCACCA CCCCACCACG GCTCTCCCCG CTCCGGCCGA
    CATGCACACT GGGGCCCCGA GCCTGCAGAG AGCGTGGAGG GTGACGGCTT CTATGACGAC
    CAAGACTATG AGTTCAATCA CCACGAGCCT CCATCCTACG AGCAAAGCCC TGTGCAGGGC
    AACCCTCACC CCCATTCCCC TAGGACTTCC CGCCACAACA CCCCTCCTCA GGGCCAAGCG
    CACCCACGGC GCATGCCAAA TGGCTACCGT TCGTCTTCGC CCTCTCCACA CCACCACGCG
    CCCGCTCACC CTGGAACTCA TAAACCTCCT CATCCCAGAG GGCCCAGAAA GGGCCTCCAT
    GAACCCTACA GTGAGACTGA CGAGGATGAC TGGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141799
    Clone ID Related Accession (Same CDS sequence) XM_021474428.1
    Accession Version XM_021474428.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6960bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG
    GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT
    GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC
    CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC
    ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT
    GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT
    GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT
    GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT
    CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA
    AGGCAGTCTC AGCAGCCTAT CACAGATAAC AGTCCTATGA AGCGCTCGGC TTCATCTTTG
    GGACACGGGC GTGCAGGACG CTCCATGCGA GGTGAGGATT ATGCCATGGA GAGGGTTATC
    CCAGAAGAGG GTCACAGGCA TGGGCATCGC CACCGAGACC GTAGTCACCG GGCGTCAGAA
    CGCTCCCTGA GCCGATACAC TGATGCTGAC ACAGGTCTTG GAACTGACCT CAGCACAACC
    ACACAATCAG GAGACCTGCC ATCCAAGGAG AGAGACCGGG GCCGAGCCAA AGACCGCAAG
    CACCATCATC ATCATCATCA CCACCACGGC TCTTTAGATA AGGAGCACTA CGGTCATGAG
    AGAGACAGAG GAGAGTACGG CCACAGACAG TCCAGAGAGA GAGACCGCCG CTGGTCCCGC
    TCACCCAGTG AGGGCAGAGA GTGTCTGACT CACAGACAGG GCAGTAGTTC AGTAAGCGGC
    AGTCCAGTCC CGTCCACCTC CGGGACCAGC ACCCCCCGCC GTGGCCGCCG TCAGCTCCCC
    CAAACCCCTG CTACACCCCG GCCACATGTC ACCTACTCAC CTGTGGTCCG TAAGCCCATC
    AGCAGTACCC CCCCTCCTGG CCAGCAGAGC CGACTCCCTA CCCCGACCTC CCGGCGCTTC
    AGTCCGACTG GCCCCGAGCC CCCGCTTCCC CCTCATCACC ACCCGTCACC ACCCCACCAC
    GGCTCTCCCC GCTCCGGCCG ACATGCACAC TGGGGCCCCG AGCCTGCAGA GAGCGTGGAG
    GGTGACGGCT TCTATGACGA CCAAGACTAT GAGTTCAATC ACCACGAGCC TCCATCCTAC
    GAGCAAAGCC CTGTGCAGGG CAACCCTCAC CCCCATTCCC CTAGGACTTC CCGCCACAAC
    ACCCCTCCTC AGGGCCAAGC GCACCCACGG CGCATGCCAA ATGGCTACCG TTCGTCTTCG
    CCCTCTCCAC ACCACCACGC GCCCGCTCAC CCTGGAACTC ATAAACCTCC TCATCCCAGA
    GGGCCCAGAA AGGGCCTCCA TGAACCCTAC AGTGAGACTG ACGAGGATGA CTGGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330103.1
    CDS1509..8468
    Translation

    Target ORF information:

    RefSeq Version XM_021474428.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474428.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG
    GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT
    GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC
    CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC
    ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT
    GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT
    GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT
    GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT
    CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA
    AGGCAGTCTC AGCAGCCTAT CACAGATAAC AGTCCTATGA AGCGCTCGGC TTCATCTTTG
    GGACACGGGC GTGCAGGACG CTCCATGCGA GGTGAGGATT ATGCCATGGA GAGGGTTATC
    CCAGAAGAGG GTCACAGGCA TGGGCATCGC CACCGAGACC GTAGTCACCG GGCGTCAGAA
    CGCTCCCTGA GCCGATACAC TGATGCTGAC ACAGGTCTTG GAACTGACCT CAGCACAACC
    ACACAATCAG GAGACCTGCC ATCCAAGGAG AGAGACCGGG GCCGAGCCAA AGACCGCAAG
    CACCATCATC ATCATCATCA CCACCACGGC TCTTTAGATA AGGAGCACTA CGGTCATGAG
    AGAGACAGAG GAGAGTACGG CCACAGACAG TCCAGAGAGA GAGACCGCCG CTGGTCCCGC
    TCACCCAGTG AGGGCAGAGA GTGTCTGACT CACAGACAGG GCAGTAGTTC AGTAAGCGGC
    AGTCCAGTCC CGTCCACCTC CGGGACCAGC ACCCCCCGCC GTGGCCGCCG TCAGCTCCCC
    CAAACCCCTG CTACACCCCG GCCACATGTC ACCTACTCAC CTGTGGTCCG TAAGCCCATC
    AGCAGTACCC CCCCTCCTGG CCAGCAGAGC CGACTCCCTA CCCCGACCTC CCGGCGCTTC
    AGTCCGACTG GCCCCGAGCC CCCGCTTCCC CCTCATCACC ACCCGTCACC ACCCCACCAC
    GGCTCTCCCC GCTCCGGCCG ACATGCACAC TGGGGCCCCG AGCCTGCAGA GAGCGTGGAG
    GGTGACGGCT TCTATGACGA CCAAGACTAT GAGTTCAATC ACCACGAGCC TCCATCCTAC
    GAGCAAAGCC CTGTGCAGGG CAACCCTCAC CCCCATTCCC CTAGGACTTC CCGCCACAAC
    ACCCCTCCTC AGGGCCAAGC GCACCCACGG CGCATGCCAA ATGGCTACCG TTCGTCTTCG
    CCCTCTCCAC ACCACCACGC GCCCGCTCAC CCTGGAACTC ATAAACCTCC TCATCCCAGA
    GGGCCCAGAA AGGGCCTCCA TGAACCCTAC AGTGAGACTG ACGAGGATGA CTGGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141800
    Clone ID Related Accession (Same CDS sequence) XM_021474432.1
    Accession Version XM_021474432.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6924bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG
    GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGAGGT GTAGATAAGC ACCAGATGGA TGCTGAATTA
    AGGAAGGAGA TGATGGCGAT ATGGCCTAAT CTCTCTCAGA AGAACCTGGA CCTGCTGGTG
    ACTCCTCACA AGTCGACAGA CCTGACAGTA GGAAAGATCT ACGCTGCCAT GATGATCATG
    GAGTATTATC GGCAAAGTAA AGCCAAACGG ACACAAGCTC TCCATGATGA GCAGCGTCTG
    GAGCCGCCAT CTCCTAGCCA GGATGTGGGA CCAGGACTCA CTGGCCTTCC TGACACACAA
    ATGCACCCTG CAAATCATCT GCCTGTTGAT GAAAGGATCC CAGAGAGCCA ATCATGGGTG
    ACAGCGCGAG CACAGGAGAT TTCTCAAAAA GCAGGCAACT GGAGTCCAGA GGGCCAACAT
    CCAGATGACA CCACAGACAA CAGAAGGCAG TCTCAGCAGC CTATCACAGA TAACAGTCCT
    ATGAAGCGCT CGGCTTCATC TTTGGGACAC GGGCGTGCAG GACGCTCCAT GCGAGGTGAG
    GATTATGCCA TGGAGAGGGT TATCCCAGAA GAGGGTCACA GGCATGGGCA TCGCCACCGA
    GACCGTAGTC ACCGGGCGTC AGAACGCTCC CTGAGCCGAT ACACTGATGC TGACACAGGT
    CTTGGAACTG ACCTCAGCAC AACCACACAA TCAGGAGACC TGCCATCCAA GGAGAGAGAC
    CGGGGCCGAG CCAAAGACCG CAAGCACCAT CATCATCATC ATCACCACCA CGGCTCTTTA
    GATAAGGAGC ACTACGGTCA TGAGAGAGAC AGAGGAGAGT ACGGCCACAG ACAGTCCAGA
    GAGAGAGACC GCCGCTGGTC CCGCTCACCC AGTGAGGGCA GAGAGTGTCT GACTCACAGA
    CAGGGCAGTA GTTCAGTAAG CGGCAGTCCA GTCCCGTCCA CCTCCGGGAC CAGCACCCCC
    CGCCGTGGCC GCCGTCAGCT CCCCCAAACC CCTGCTACAC CCCGGCCACA TGTCACCTAC
    TCACCTGTGG TCCGTAAGCC CATCAGCAGT ACCCCCCCTC CTGGCCAGCA GAGCCGACTC
    CCTACCCCGA CCTCCCGGCG CTTCAGTCCG ACTGGCCCCG AGCCCCCGCT TCCCCCTCAT
    CACCACCCGT CACCACCCCA CCACGGCTCT CCCCGCTCCG GCCGACATGC ACACTGGGGC
    CCCGAGCCTG CAGAGAGCGT GGAGGGTGAC GGCTTCTATG ACGACCAAGA CTATGAGTTC
    AATCACCACG AGCCTCCATC CTACGAGCAA AGCCCTGTGC AGGGCAACCC TCACCCCCAT
    TCCCCTAGGA CTTCCCGCCA CAACACCCCT CCTCAGGGCC AAGCGCACCC ACGGCGCATG
    CCAAATGGCT ACCGTTCGTC TTCGCCCTCT CCACACCACC ACGCGCCCGC TCACCCTGGA
    ACTCATAAAC CTCCTCATCC CAGAGGGCCC AGAAAGGGCC TCCATGAACC CTACAGTGAG
    ACTGACGAGG ATGACTGGTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330107.1
    CDS1512..8435
    Translation

    Target ORF information:

    RefSeq Version XM_021474432.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474432.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG
    GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGAGGT GTAGATAAGC ACCAGATGGA TGCTGAATTA
    AGGAAGGAGA TGATGGCGAT ATGGCCTAAT CTCTCTCAGA AGAACCTGGA CCTGCTGGTG
    ACTCCTCACA AGTCGACAGA CCTGACAGTA GGAAAGATCT ACGCTGCCAT GATGATCATG
    GAGTATTATC GGCAAAGTAA AGCCAAACGG ACACAAGCTC TCCATGATGA GCAGCGTCTG
    GAGCCGCCAT CTCCTAGCCA GGATGTGGGA CCAGGACTCA CTGGCCTTCC TGACACACAA
    ATGCACCCTG CAAATCATCT GCCTGTTGAT GAAAGGATCC CAGAGAGCCA ATCATGGGTG
    ACAGCGCGAG CACAGGAGAT TTCTCAAAAA GCAGGCAACT GGAGTCCAGA GGGCCAACAT
    CCAGATGACA CCACAGACAA CAGAAGGCAG TCTCAGCAGC CTATCACAGA TAACAGTCCT
    ATGAAGCGCT CGGCTTCATC TTTGGGACAC GGGCGTGCAG GACGCTCCAT GCGAGGTGAG
    GATTATGCCA TGGAGAGGGT TATCCCAGAA GAGGGTCACA GGCATGGGCA TCGCCACCGA
    GACCGTAGTC ACCGGGCGTC AGAACGCTCC CTGAGCCGAT ACACTGATGC TGACACAGGT
    CTTGGAACTG ACCTCAGCAC AACCACACAA TCAGGAGACC TGCCATCCAA GGAGAGAGAC
    CGGGGCCGAG CCAAAGACCG CAAGCACCAT CATCATCATC ATCACCACCA CGGCTCTTTA
    GATAAGGAGC ACTACGGTCA TGAGAGAGAC AGAGGAGAGT ACGGCCACAG ACAGTCCAGA
    GAGAGAGACC GCCGCTGGTC CCGCTCACCC AGTGAGGGCA GAGAGTGTCT GACTCACAGA
    CAGGGCAGTA GTTCAGTAAG CGGCAGTCCA GTCCCGTCCA CCTCCGGGAC CAGCACCCCC
    CGCCGTGGCC GCCGTCAGCT CCCCCAAACC CCTGCTACAC CCCGGCCACA TGTCACCTAC
    TCACCTGTGG TCCGTAAGCC CATCAGCAGT ACCCCCCCTC CTGGCCAGCA GAGCCGACTC
    CCTACCCCGA CCTCCCGGCG CTTCAGTCCG ACTGGCCCCG AGCCCCCGCT TCCCCCTCAT
    CACCACCCGT CACCACCCCA CCACGGCTCT CCCCGCTCCG GCCGACATGC ACACTGGGGC
    CCCGAGCCTG CAGAGAGCGT GGAGGGTGAC GGCTTCTATG ACGACCAAGA CTATGAGTTC
    AATCACCACG AGCCTCCATC CTACGAGCAA AGCCCTGTGC AGGGCAACCC TCACCCCCAT
    TCCCCTAGGA CTTCCCGCCA CAACACCCCT CCTCAGGGCC AAGCGCACCC ACGGCGCATG
    CCAAATGGCT ACCGTTCGTC TTCGCCCTCT CCACACCACC ACGCGCCCGC TCACCCTGGA
    ACTCATAAAC CTCCTCATCC CAGAGGGCCC AGAAAGGGCC TCCATGAACC CTACAGTGAG
    ACTGACGAGG ATGACTGGTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141801
    Clone ID Related Accession (Same CDS sequence) XM_021474433.1
    Accession Version XM_021474433.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6498bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X23
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG
    GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT
    GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC
    CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC
    ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT
    GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT
    GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT
    GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT
    CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA
    AGGCAGTCTC AGACAGTAGA GATGAGAGAG ATGGGGCGGG ACGGGTACTC GGACACAGAG
    CAATATCTGC CGATGGAAGG CCATGGCAGG GCTGCTTCCA TGCCTCGACT CCCAGCAGAC
    AACCAACAGC CACGGAGGAA AGGGCGTCAC CGGGGAGAAA ATCTCACTCA GCCTATCACA
    GATAACAGTC CTATGAAGCG CTCGGCTTCA TCTTTGGGAC ACGGGCGTGC AGGACGCTCC
    ATGCGAGGTG AGGATTATGC CATGGAGAGG GTTATCCCAG AAGAGGGTCA CAGGCATGGG
    CATCGCCACC GAGACCGTAG TCACCGGGCG TCAGAACGCT CCCTGAGCCG ATACACTGAT
    GCTGACACAG GTCTTGGAAC TGACCTCAGC ACAACCACAC AATCAGGAGA CCTGCCATCC
    AAGGAGAGAG ACCGGGGCCG AGCCAAAGAC CGCAAGCACC ATCATCATCA TCATCACCAC
    CACGGCTCTT TAGATAAGGA GCACTACGGT CATGAGAGAG ACAGAGGAGA GTACGGCCAC
    AGACAGTCCA GAGAGAGAGA CCGCCGCTGG TCCCGCTCAC CCAGTGAGGG CAGAGAGTGT
    CTGACTCACA GACAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330108.1
    CDS1519..8016
    Translation

    Target ORF information:

    RefSeq Version XM_021474433.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474433.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG
    GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT
    GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC
    CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC
    ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT
    GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT
    GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT
    GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT
    CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA
    AGGCAGTCTC AGACAGTAGA GATGAGAGAG ATGGGGCGGG ACGGGTACTC GGACACAGAG
    CAATATCTGC CGATGGAAGG CCATGGCAGG GCTGCTTCCA TGCCTCGACT CCCAGCAGAC
    AACCAACAGC CACGGAGGAA AGGGCGTCAC CGGGGAGAAA ATCTCACTCA GCCTATCACA
    GATAACAGTC CTATGAAGCG CTCGGCTTCA TCTTTGGGAC ACGGGCGTGC AGGACGCTCC
    ATGCGAGGTG AGGATTATGC CATGGAGAGG GTTATCCCAG AAGAGGGTCA CAGGCATGGG
    CATCGCCACC GAGACCGTAG TCACCGGGCG TCAGAACGCT CCCTGAGCCG ATACACTGAT
    GCTGACACAG GTCTTGGAAC TGACCTCAGC ACAACCACAC AATCAGGAGA CCTGCCATCC
    AAGGAGAGAG ACCGGGGCCG AGCCAAAGAC CGCAAGCACC ATCATCATCA TCATCACCAC
    CACGGCTCTT TAGATAAGGA GCACTACGGT CATGAGAGAG ACAGAGGAGA GTACGGCCAC
    AGACAGTCCA GAGAGAGAGA CCGCCGCTGG TCCCGCTCAC CCAGTGAGGG CAGAGAGTGT
    CTGACTCACA GACAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141802
    Clone ID Related Accession (Same CDS sequence) XM_021474440.1
    Accession Version XM_021474440.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4848bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X25
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGT TTTTTTGTTA TAATCAACCT
    TTATTTGGAT CCAGAGTTTG TTGTAAGATA TGCATAATGA TAATTCATTT CTTGTTTCAT
    CTTTTTTTAC ATGCTTTCTT TAAACTCTTC TTATTGGCGG CTTCGCCCTC TGATGACTCC
    ATCCAATCAA AATGCACTAT GAAACTCATG TGTATATCCA TGACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330115.1
    CDS1556..6403
    Translation

    Target ORF information:

    RefSeq Version XM_021474440.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474440.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGT TTTTTTGTTA TAATCAACCT
    TTATTTGGAT CCAGAGTTTG TTGTAAGATA TGCATAATGA TAATTCATTT CTTGTTTCAT
    CTTTTTTTAC ATGCTTTCTT TAAACTCTTC TTATTGGCGG CTTCGCCCTC TGATGACTCC
    ATCCAATCAA AATGCACTAT GAAACTCATG TGTATATCCA TGACCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141803
    Clone ID Related Accession (Same CDS sequence) XM_021474441.1
    Accession Version XM_021474441.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7035bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X27
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCTAAGGA GCAGCAGAAG AACCACAAAG GTTGTAAGTC GGTATGGGAG
    CAGCGCACTA GTGAGTTAAG ACGACAGACC CTCGTGAACA GCAGAGAGGC TCTTTATAAT
    GAGTTGGACC CTGAAGACCG TTGGAAGGTC TCATACTCCC GTCACATACG TCCCGACATG
    AAAACTCACC TGGACCGGCC GTTAGTCGTG GACCCTCAGG AGAACCGCAA TAACAACACT
    AACAAAACCC GTCCGGGTGA CGGCCAACCT CAGCATTCCC ATCAGCTCCT ACACAAACAG
    CCCAACTTCA ATGAGGCCGA GACTGGAGGA GGTGGAGGGT CTGAGTCCTG GCCCGCACTG
    GAAAGAGGAG ACTCAACAGG GTCCCGCCGC AGTCTTTTGG GCTCTGAGCA CTATGAGGAG
    GGTAGAGAGT CCCACCGCAG ACACCAGCAC AGCCAGCACT GCAGTCAGGC CCGTCAACAT
    CGCAAAGCTG CAGAGGGCGG TGGAGAGCCT GGAGGGAGGA GACACAGGAG TAAGGCCAAG
    GAAAGAGGAC CTGAGTGTGA GCATTCAGAT GGAGGGGAGC GGAAACACAG ACGGCATCGG
    CATGAGGGTG AGGGGAGGAA AGAGAGAGGG GTACGACACC GAAACAGGAG GGATGGCCAT
    GCAAGTGGCC CGACTCTCTC TACAACCCGT CCCATTCAGA AGACTCTGTC CAGGCAGGAC
    AGTCAGTACA GCGAAGACCT GGACAATGCC ATGAACAACA AACTTGCTAC CCAGCCACAT
    GATAGTCTGC TTAATCTGGC CAACACTGCC CACTCTGCCG GCCTGGCACA TACTGGTACA
    GAGAGCAGCC TCATCCTCAC CAACCCTTCC TCTACCATTG CAAATGTTAC TAGCATCGGT
    CACCTGGGTA TGAAACCTGA GTACACTGCC GTGGACATTC CTCCCATGTT CCCCTCCAGT
    AATGCCATTC TACAGGTCAA TAAGAATGCC AACACGGAGC CTCTGCCAAA GAAGGAAGAC
    ACAAAGGGTG ACGATGATGA TGATGAGAAA GATGACGGAG GGCCTAAACC CATGCCACCC
    TACACCTCAA TGTTCATTCT GACCACCACC AACCCATTTC GGAGATTGTG TCACTATATC
    GTCACACTGA GATATTTTGA GATGTGTATT CTACTGGTTA TTGCTATGAG CAGTATTGCC
    CTGGCTGCTG AAGACCCTGT CTGGCCTGAA TCTCCCCGCA ACAATGTCCT ACGATACTTT
    GACTATGTGT TCACTGGTGT GTTCACCTTT GAGATGCTCA TTAAGATGGT AGATTTAGGT
    CTTGTCCTGC ATCAAGGCTC ATACTTTCGT GATCTGTGGA ACATCCTGGA CTTTATAGTG
    GTTAGTGGTG CCCTGGTGGC GTTCGCCTTC ACAGGAAGCA GCAAAGGGAA AGACATCAGT
    ACTATCAAGT CTCTACGTGT GCTTCGAGTA CTGCGGCCGC TGAAAACCAT CAAACGTCTG
    CCAAAACTGA AGGCTGTCTT TGACTGTGTG GTGAACTCTC TGAAGAATGT GTTGAACATC
    CTCATCGTCT ACATGCTCTT CATGTTCATC TTCGCTGTGG TGGCTGTGCA GCTCTTTAAG
    GGACGTTTCT TTTACTGCAC TGATGAATCC AAAGAGTTTG AGCGCGACTG CAGGGGCGAG
    TATCTGGTCT ATGAAAGAGA TAACGAAGTG AGGTCACAGA AGCGGGAATG GAAGAAATAC
    GATTTCCACT ACGACAACGT GCTCTGGGCC CTTCTCACCC TCTTCACCGT TTCTACGGGA
    GAGGGGTGGC CGCAAGTGCT AAAACATTCA GTAGATGCGA CCTACGAGAA TCAGGGCCCG
    AGCCCAGGTT ACCGGATGGA AATGTCCATC TTTTACGTGG TGTACTTCGT GGTCTTCCCC
    TTCTTCTTCG TCAACATCTT CGTGGCTCTT ATCATCATTA CTTTCCAGGA GCAAGGAGAC
    AAAATGATGG AGGACTATAG CCTGGAAAAG AATGAGAGAG CCTGCATAGA CTTTGCCATC
    AACGCTAAGC CGCTCACACG GCACATGCCC CAGAACAAGC AGACCTTTCA GTACCGCATG
    TGGGAGTTTG TGGTGTCTCC TCCATTTGAA TACACCATCA TGGCCTTGAT CGCGCTCAAC
    ACTATTGTCC TTATGATGAA GTATGATGGA GCATCGCTTA CTTATGAAGA TGTGCTAAAA
    TACCTGAACA TAGTGTTCAC CTCTCTATTC TCCATGGAGT GCATATTGAA GATCATAGCC
    TTCGGAGCAT TGAACTACTT CAAAGATGCC TGGAACATCT TCGACTGTGT GACTGTACTT
    GGCAGCATCA CTGATATTTT AGTCACAGAG CTGGGGAACA ACTTCATAAA TCTGAGTTTT
    CTAAGGCTCT TCAGAGCTGC TCGTCTCATT AAGTTGCTCA GGCAGGGTGA AACCATACGC
    ATCCTGCTCT GGACTTTTGT CCAGTCTTTC AAGGCTCTGC CGTATGTGTG CCTACTCATC
    GCCATGCTGT TCTTCATCTA CGCCATCATT GGCATGCAGC TGTTTGGGAA CATTAAGATC
    GAGGAGAACA GTGACAGTGC CATTACCCAG CACAACAACT TCAGGACTTT TTTCCAGGCC
    CTAATGCTGC TCTTTAGGAG TGCTACAGGT GAGGCATGGC ATGACATCAT GCTGTCATGT
    CTGGGAAAGA AGCCGTGTGA TATTCTCTCT GATAACCCGA AGCCAGAATG CGGGAGCGAG
    TTTGCCTACC TCTACTTTGT CTCCTTCATC TTCCTCTGTT CATTTCTGAT GCTGAATCTG
    TTCGTGGCTG TGATCATGGA TAATTTTGAA TACCTGACGC GTGACTCCTC TATCCTGGGA
    CCCCATCACC TGGACGAGTA TGTGAGGATC TGGGCAGAAT ATGATCCTGC TGCTTGTGGC
    AGAATCAGTT ACAGGGATAT GTATGAGATG CTCAGGCACA TGTCTCCTCC GTTAGGCCTG
    GGGAAGAAGT GCCCTGCACG GGTTGCCTAC AAGAGACTGT TGCGGATGGA TCTGCCGGTT
    GCCGATGACA ACACAGTGCA CTTTAATTCT ACCCTTATGG CCTTAATCCG AACCGCTCTG
    GACATCAAGA TCGCCAAGGG AGGTGTAGAT AAGCACCAGA TGGATGCTGA ATTAAGGAAG
    GAGATGATGG CGATATGGCC TAATCTCTCT CAGAAGAACC TGGACCTGCT GGTGACTCCT
    CACAAGTCGA CAGACCTGAC AGTAGGAAAG ATCTACGCTG CCATGATGAT CATGGAGTAT
    TATCGGCAAA GTAAAGCCAA ACGGACACAA GCTCTCCATG ATGAGCAGAA TCGAACGCCA
    TTAATGTTTC AGCGTCTGGA GCCGCCATCT CCTAGCCAGG ATGTGGGACC AGGACTCACT
    GGCCTTCCTG ACACACAAAT GCACCCTGCA AATCATCTGC CTGTTGATGA AAGGATCCCA
    GAGAGCCAAT CATGGGTGAC AGCGCGAGCA CAGGAGATTT CTCAAAAAGC AGGCAACTGG
    AGTCCAGAGG GCCAACATCC AGATGACACC ACAGACAACA GAAGGCAGTC TCAGACAGTA
    GAGATGAGAG AGATGGGGCG GGACGGGTAC TCGGACACAG AGCAATATCT GCCGATGGAA
    GGCCATGGCA GGGCTGCTTC CATGCCTCGA CTCCCAGCAG ACAACCAACA GCCACGGAGG
    AAAGGGCGTC ACCGGGGAGA AAATCTCACT CCTATCACAG ATAACAGTCC TATGAAGCGC
    TCGGCTTCAT CTTTGGGACA CGGGCGTGCA GGACGCTCCA TGCGAGGTGA GGATTATGCC
    ATGGAGAGGG TTATCCCAGA AGAGGGTCAC AGGCATGGGC ATCGCCACCG AGACCGTAGT
    CACCGGGCGT CAGAACGCTC CCTGAGCCGA TACACTGATG CTGACACAGG TCTTGGAACT
    GACCTCAGCA CAACCACACA ATCAGGAGAC CTGCCATCCA AGGAGAGAGA CCGGGGCCGA
    GCCAAAGACC GCAAGCACCA TCATCATCAT CATCACCACC ACGGCTCTTT AGATAAGGAG
    CACTACGGTC ATGAGAGAGA CAGAGGAGAG TACGGCCACA GACAGTCCAG AGAGAGAGAC
    CGCCGCTGGT CCCGCTCACC CAGTGAGGGC AGAGAGTGTC TGACTCACAG ACAGGGCAGT
    AGTTCAGTAA GCGGCAGTCC AGTCCCGTCC ACCTCCGGGA CCAGCACCCC CCGCCGTGGC
    CGCCGTCAGC TCCCCCAAAC CCCTGCTACA CCCCGGCCAC ATGTCACCTA CTCACCTGTG
    GTCCGTAAGC CCATCAGCAG TACCCCCCCT CCTGGCCAGC AGAGCCGACT CCCTACCCCG
    ACCTCCCGGC GCTTCAGTCC GACTGGCCCC GAGCCCCCGC TTCCCCCTCA TCACCACCCG
    TCACCACCCC ACCACGGCTC TCCCCGCTCC GGCCGACATG CACACTGGGG CCCCGAGCCT
    GCAGAGAGCG TGGAGGGTGA CGGCTTCTAT GACGACCAAG ACTATGAGTT CAATCACCAC
    GAGCCTCCAT CCTACGAGCA AAGCCCTGTG CAGGGCAACC CTCACCCCCA TTCCCCTAGG
    ACTTCCCGCC ACAACACCCC TCCTCAGGGC CAAGCGCACC CACGGCGCAT GCCAAATGGC
    TACCGTTCGT CTTCGCCCTC TCCACACCAC CACGCGCCCG CTCACCCTGG AACTCATAAA
    CCTCCTCATC CCAGAGGGCC CAGAAAGGGC CTCCATGAAC CCTACAGTGA GACTGACGAG
    GATGACTGGT GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330116.1
    CDS1508..8542
    Translation

    Target ORF information:

    RefSeq Version XM_021474441.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X27, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474441.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCTAAGGA GCAGCAGAAG AACCACAAAG GTTGTAAGTC GGTATGGGAG
    CAGCGCACTA GTGAGTTAAG ACGACAGACC CTCGTGAACA GCAGAGAGGC TCTTTATAAT
    GAGTTGGACC CTGAAGACCG TTGGAAGGTC TCATACTCCC GTCACATACG TCCCGACATG
    AAAACTCACC TGGACCGGCC GTTAGTCGTG GACCCTCAGG AGAACCGCAA TAACAACACT
    AACAAAACCC GTCCGGGTGA CGGCCAACCT CAGCATTCCC ATCAGCTCCT ACACAAACAG
    CCCAACTTCA ATGAGGCCGA GACTGGAGGA GGTGGAGGGT CTGAGTCCTG GCCCGCACTG
    GAAAGAGGAG ACTCAACAGG GTCCCGCCGC AGTCTTTTGG GCTCTGAGCA CTATGAGGAG
    GGTAGAGAGT CCCACCGCAG ACACCAGCAC AGCCAGCACT GCAGTCAGGC CCGTCAACAT
    CGCAAAGCTG CAGAGGGCGG TGGAGAGCCT GGAGGGAGGA GACACAGGAG TAAGGCCAAG
    GAAAGAGGAC CTGAGTGTGA GCATTCAGAT GGAGGGGAGC GGAAACACAG ACGGCATCGG
    CATGAGGGTG AGGGGAGGAA AGAGAGAGGG GTACGACACC GAAACAGGAG GGATGGCCAT
    GCAAGTGGCC CGACTCTCTC TACAACCCGT CCCATTCAGA AGACTCTGTC CAGGCAGGAC
    AGTCAGTACA GCGAAGACCT GGACAATGCC ATGAACAACA AACTTGCTAC CCAGCCACAT
    GATAGTCTGC TTAATCTGGC CAACACTGCC CACTCTGCCG GCCTGGCACA TACTGGTACA
    GAGAGCAGCC TCATCCTCAC CAACCCTTCC TCTACCATTG CAAATGTTAC TAGCATCGGT
    CACCTGGGTA TGAAACCTGA GTACACTGCC GTGGACATTC CTCCCATGTT CCCCTCCAGT
    AATGCCATTC TACAGGTCAA TAAGAATGCC AACACGGAGC CTCTGCCAAA GAAGGAAGAC
    ACAAAGGGTG ACGATGATGA TGATGAGAAA GATGACGGAG GGCCTAAACC CATGCCACCC
    TACACCTCAA TGTTCATTCT GACCACCACC AACCCATTTC GGAGATTGTG TCACTATATC
    GTCACACTGA GATATTTTGA GATGTGTATT CTACTGGTTA TTGCTATGAG CAGTATTGCC
    CTGGCTGCTG AAGACCCTGT CTGGCCTGAA TCTCCCCGCA ACAATGTCCT ACGATACTTT
    GACTATGTGT TCACTGGTGT GTTCACCTTT GAGATGCTCA TTAAGATGGT AGATTTAGGT
    CTTGTCCTGC ATCAAGGCTC ATACTTTCGT GATCTGTGGA ACATCCTGGA CTTTATAGTG
    GTTAGTGGTG CCCTGGTGGC GTTCGCCTTC ACAGGAAGCA GCAAAGGGAA AGACATCAGT
    ACTATCAAGT CTCTACGTGT GCTTCGAGTA CTGCGGCCGC TGAAAACCAT CAAACGTCTG
    CCAAAACTGA AGGCTGTCTT TGACTGTGTG GTGAACTCTC TGAAGAATGT GTTGAACATC
    CTCATCGTCT ACATGCTCTT CATGTTCATC TTCGCTGTGG TGGCTGTGCA GCTCTTTAAG
    GGACGTTTCT TTTACTGCAC TGATGAATCC AAAGAGTTTG AGCGCGACTG CAGGGGCGAG
    TATCTGGTCT ATGAAAGAGA TAACGAAGTG AGGTCACAGA AGCGGGAATG GAAGAAATAC
    GATTTCCACT ACGACAACGT GCTCTGGGCC CTTCTCACCC TCTTCACCGT TTCTACGGGA
    GAGGGGTGGC CGCAAGTGCT AAAACATTCA GTAGATGCGA CCTACGAGAA TCAGGGCCCG
    AGCCCAGGTT ACCGGATGGA AATGTCCATC TTTTACGTGG TGTACTTCGT GGTCTTCCCC
    TTCTTCTTCG TCAACATCTT CGTGGCTCTT ATCATCATTA CTTTCCAGGA GCAAGGAGAC
    AAAATGATGG AGGACTATAG CCTGGAAAAG AATGAGAGAG CCTGCATAGA CTTTGCCATC
    AACGCTAAGC CGCTCACACG GCACATGCCC CAGAACAAGC AGACCTTTCA GTACCGCATG
    TGGGAGTTTG TGGTGTCTCC TCCATTTGAA TACACCATCA TGGCCTTGAT CGCGCTCAAC
    ACTATTGTCC TTATGATGAA GTATGATGGA GCATCGCTTA CTTATGAAGA TGTGCTAAAA
    TACCTGAACA TAGTGTTCAC CTCTCTATTC TCCATGGAGT GCATATTGAA GATCATAGCC
    TTCGGAGCAT TGAACTACTT CAAAGATGCC TGGAACATCT TCGACTGTGT GACTGTACTT
    GGCAGCATCA CTGATATTTT AGTCACAGAG CTGGGGAACA ACTTCATAAA TCTGAGTTTT
    CTAAGGCTCT TCAGAGCTGC TCGTCTCATT AAGTTGCTCA GGCAGGGTGA AACCATACGC
    ATCCTGCTCT GGACTTTTGT CCAGTCTTTC AAGGCTCTGC CGTATGTGTG CCTACTCATC
    GCCATGCTGT TCTTCATCTA CGCCATCATT GGCATGCAGC TGTTTGGGAA CATTAAGATC
    GAGGAGAACA GTGACAGTGC CATTACCCAG CACAACAACT TCAGGACTTT TTTCCAGGCC
    CTAATGCTGC TCTTTAGGAG TGCTACAGGT GAGGCATGGC ATGACATCAT GCTGTCATGT
    CTGGGAAAGA AGCCGTGTGA TATTCTCTCT GATAACCCGA AGCCAGAATG CGGGAGCGAG
    TTTGCCTACC TCTACTTTGT CTCCTTCATC TTCCTCTGTT CATTTCTGAT GCTGAATCTG
    TTCGTGGCTG TGATCATGGA TAATTTTGAA TACCTGACGC GTGACTCCTC TATCCTGGGA
    CCCCATCACC TGGACGAGTA TGTGAGGATC TGGGCAGAAT ATGATCCTGC TGCTTGTGGC
    AGAATCAGTT ACAGGGATAT GTATGAGATG CTCAGGCACA TGTCTCCTCC GTTAGGCCTG
    GGGAAGAAGT GCCCTGCACG GGTTGCCTAC AAGAGACTGT TGCGGATGGA TCTGCCGGTT
    GCCGATGACA ACACAGTGCA CTTTAATTCT ACCCTTATGG CCTTAATCCG AACCGCTCTG
    GACATCAAGA TCGCCAAGGG AGGTGTAGAT AAGCACCAGA TGGATGCTGA ATTAAGGAAG
    GAGATGATGG CGATATGGCC TAATCTCTCT CAGAAGAACC TGGACCTGCT GGTGACTCCT
    CACAAGTCGA CAGACCTGAC AGTAGGAAAG ATCTACGCTG CCATGATGAT CATGGAGTAT
    TATCGGCAAA GTAAAGCCAA ACGGACACAA GCTCTCCATG ATGAGCAGAA TCGAACGCCA
    TTAATGTTTC AGCGTCTGGA GCCGCCATCT CCTAGCCAGG ATGTGGGACC AGGACTCACT
    GGCCTTCCTG ACACACAAAT GCACCCTGCA AATCATCTGC CTGTTGATGA AAGGATCCCA
    GAGAGCCAAT CATGGGTGAC AGCGCGAGCA CAGGAGATTT CTCAAAAAGC AGGCAACTGG
    AGTCCAGAGG GCCAACATCC AGATGACACC ACAGACAACA GAAGGCAGTC TCAGACAGTA
    GAGATGAGAG AGATGGGGCG GGACGGGTAC TCGGACACAG AGCAATATCT GCCGATGGAA
    GGCCATGGCA GGGCTGCTTC CATGCCTCGA CTCCCAGCAG ACAACCAACA GCCACGGAGG
    AAAGGGCGTC ACCGGGGAGA AAATCTCACT CCTATCACAG ATAACAGTCC TATGAAGCGC
    TCGGCTTCAT CTTTGGGACA CGGGCGTGCA GGACGCTCCA TGCGAGGTGA GGATTATGCC
    ATGGAGAGGG TTATCCCAGA AGAGGGTCAC AGGCATGGGC ATCGCCACCG AGACCGTAGT
    CACCGGGCGT CAGAACGCTC CCTGAGCCGA TACACTGATG CTGACACAGG TCTTGGAACT
    GACCTCAGCA CAACCACACA ATCAGGAGAC CTGCCATCCA AGGAGAGAGA CCGGGGCCGA
    GCCAAAGACC GCAAGCACCA TCATCATCAT CATCACCACC ACGGCTCTTT AGATAAGGAG
    CACTACGGTC ATGAGAGAGA CAGAGGAGAG TACGGCCACA GACAGTCCAG AGAGAGAGAC
    CGCCGCTGGT CCCGCTCACC CAGTGAGGGC AGAGAGTGTC TGACTCACAG ACAGGGCAGT
    AGTTCAGTAA GCGGCAGTCC AGTCCCGTCC ACCTCCGGGA CCAGCACCCC CCGCCGTGGC
    CGCCGTCAGC TCCCCCAAAC CCCTGCTACA CCCCGGCCAC ATGTCACCTA CTCACCTGTG
    GTCCGTAAGC CCATCAGCAG TACCCCCCCT CCTGGCCAGC AGAGCCGACT CCCTACCCCG
    ACCTCCCGGC GCTTCAGTCC GACTGGCCCC GAGCCCCCGC TTCCCCCTCA TCACCACCCG
    TCACCACCCC ACCACGGCTC TCCCCGCTCC GGCCGACATG CACACTGGGG CCCCGAGCCT
    GCAGAGAGCG TGGAGGGTGA CGGCTTCTAT GACGACCAAG ACTATGAGTT CAATCACCAC
    GAGCCTCCAT CCTACGAGCA AAGCCCTGTG CAGGGCAACC CTCACCCCCA TTCCCCTAGG
    ACTTCCCGCC ACAACACCCC TCCTCAGGGC CAAGCGCACC CACGGCGCAT GCCAAATGGC
    TACCGTTCGT CTTCGCCCTC TCCACACCAC CACGCGCCCG CTCACCCTGG AACTCATAAA
    CCTCCTCATC CCAGAGGGCC CAGAAAGGGC CTCCATGAAC CCTACAGTGA GACTGACGAG
    GATGACTGGT GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141804
    Clone ID Related Accession (Same CDS sequence) XM_021474442.1
    Accession Version XM_021474442.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7035bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X28
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCTAAGGA GCAGCAGAAG AACCACAAAG GTTGTAAGTC GGTATGGGAG
    CAGCGCACTA GTGAGTTAAG ACGACAGACC CTCGTGAACA GCAGAGAGGC TCTTTATAAT
    GAGTTGGACC CTGAAGACCG TTGGAAGGTC TCATACTCCC GTCACATACG TCCCGACATG
    AAAACTCACC TGGACCGGCC GTTAGTCGTG GACCCTCAGG AGAACCGCAA TAACAACACT
    AACAAAACCC GTCCGGGTGA CGGCCAACCT CAGCATTCCC ATCAGCTCCT ACACAAACAG
    CCCAACTTCA ATGAGGCCGA GACTGGAGGA GGTGGAGGGT CTGAGTCCTG GCCCGCACTG
    GAAAGAGGAG ACTCAACAGG GTCCCGCCGC AGTCTTTTGG GCTCTGAGCA CTATGAGGAG
    GGTAGAGAGT CCCACCGCAG ACACCAGCAC AGCCAGCACT GCAGTCAGGC CCGTCAACAT
    CGCAAAGCTG CAGAGGGCGG TGGAGAGCCT GGAGGGAGGA GACACAGGAG TAAGGCCAAG
    GAAAGAGGAC CTGAGTGTGA GCATTCAGAT GGAGGGGAGC GGAAACACAG ACGGCATCGG
    CATGAGGGTG AGGGGAGGAA AGAGAGAGGG GTACGACACC GAAACAGGAG GGATGGCCAT
    GCAAGTGGCC CGACTCTCTC TACAACCCGT CCCATTCAGA AGACTCTGTC CAGGCAGGAC
    AGTCAGTACA GCGAAGACCT GGACAATGCC ATGAACAACA AACTTGCTAC CCAGCCACAT
    GATAGTCTGC TTAATCTGGC CAACACTGCC CACTCTGCCG GCCTGGCACA TACTGGTACA
    GAGAGCAGCC TCATCCTCAC CAACCCTTCC TCTACCATTG CAAATGTTAC TAGCATCGGT
    CACCTGGGTA TGAAACCTGA GTACACTGCC GTGGACATTC CTCCCATGTT CCCCTCCAGT
    AATGCCATTC TACAGGTCAA TAAGAATGCC AACACGGAGC CTCTGCCAAA GAAGGAAGAC
    ACAAAGGGTG ACGATGATGA TGATGAGAAA GATGACGGAG GGCCTAAACC CATGCCACCC
    TACACCTCAA TGTTCATTCT GACCACCACC AACCCATTTC GGAGATTGTG TCACTATATC
    GTCACACTGA GATATTTTGA GATGTGTATT CTACTGGTTA TTGCTATGAG CAGTATTGCC
    CTGGCTGCTG AAGACCCTGT CTGGCCTGAA TCTCCCCGCA ACAATGTCCT ACGATACTTT
    GACTATGTGT TCACTGGTGT GTTCACCTTT GAGATGCTCA TTAAGATGGT AGATTTAGGT
    CTTGTCCTGC ATCAAGGCTC ATACTTTCGT GATCTGTGGA ACATCCTGGA CTTTATAGTG
    GTTAGTGGTG CCCTGGTGGC GTTCGCCTTC ACAGGAAGCA GCAAAGGGAA AGACATCAGT
    ACTATCAAGT CTCTACGTGT GCTTCGAGTA CTGCGGCCGC TGAAAACCAT CAAACGTCTG
    CCAAAACTGA AGGCTGTCTT TGACTGTGTG GTGAACTCTC TGAAGAATGT GTTGAACATC
    CTCATCGTCT ACATGCTCTT CATGTTCATC TTCGCTGTGG TGGCTGTGCA GCTCTTTAAG
    GGACGTTTCT TTTACTGCAC TGATGAATCC AAAGAGTTTG AGCGCGACTG CAGGGGCGAG
    TATCTGGTCT ATGAAAGAGA TAACGAAGTG AGGTCACAGA AGCGGGAATG GAAGAAATAC
    GATTTCCACT ACGACAACGT GCTCTGGGCC CTTCTCACCC TCTTCACCGT TTCTACGGGA
    GAGGGGTGGC CGCAAGTGCT AAAACATTCA GTAGATGCGA CCTACGAGAA TCAGGGCCCG
    AGCCCAGGTT ACCGGATGGA AATGTCCATC TTTTACGTGG TGTACTTCGT GGTCTTCCCC
    TTCTTCTTCG TCAACATCTT CGTGGCTCTT ATCATCATTA CTTTCCAGGA GCAAGGAGAC
    AAAATGATGG AGGACTATAG CCTGGAAAAG AATGAGAGAG CCTGCATAGA CTTTGCCATC
    AACGCTAAGC CGCTCACACG GCACATGCCC CAGAACAAGC AGACCTTTCA GTACCGCATG
    TGGGAGTTTG TGGTGTCTCC TCCATTTGAA TACACCATCA TGGCCTTGAT CGCGCTCAAC
    ACTATTGTCC TTATGATGAA GTATGATGGA GCATCGCTTA CTTATGAAGA TGTGCTAAAA
    TACCTGAACA TAGTGTTCAC CTCTCTATTC TCCATGGAGT GCATATTGAA GATCATAGCC
    TTCGGAGCAT TGAACTACTT CAAAGATGCC TGGAACATCT TCGACTGTGT GACTGTACTT
    GGCAGCATCA CTGATATTTT AGTCACAGAG CTGGGGAACA ACTTCATAAA TCTGAGTTTT
    CTAAGGCTCT TCAGAGCTGC TCGTCTCATT AAGTTGCTCA GGCAGGGTGA AACCATACGC
    ATCCTGCTCT GGACTTTTGT CCAGTCTTTC AAGGCTCTGC CGTATGTGTG CCTACTCATC
    GCCATGCTGT TCTTCATCTA CGCCATCATT GGCATGCAGC TGTTTGGGAA CATTAAGATC
    GAGGAGAACA GTGACAGTGC CATTACCCAG CACAACAACT TCAGGACTTT TTTCCAGGCC
    CTAATGCTGC TCTTTAGGAG TGCTACAGGT GAGGCATGGC ATGACATCAT GCTGTCATGT
    CTGGGAAAGA AGCCGTGTGA TATTCTCTCT GATAACCCGA AGCCAGAATG CGGGAGCGAG
    TTTGCCTACC TCTACTTTGT CTCCTTCATC TTCCTCTGTT CATTTCTGAT GCTGAATCTG
    TTCGTGGCTG TGATCATGGA TAATTTTGAA TACCTGACGC GTGACTCCTC TATCCTGGGA
    CCCCATCACC TGGACGAGTA TGTGAGGATC TGGGCAGAAT ATGATCCTGC TGCTTGCGGG
    CGCATTCATT ATAAGGATAT GTACAGTTTA TTGCGAGTTA TCGACCCCCC TCTGGGCTTA
    GGCAAGAAAT GTCCTCATAG GGTGGCCTGC AAGAGACTGT TGCGGATGGA TCTGCCGGTT
    GCCGATGACA ACACAGTGCA CTTTAATTCT ACCCTTATGG CCTTAATCCG AACCGCTCTG
    GACATCAAGA TCGCCAAGGG AGGTGTAGAT AAGCACCAGA TGGATGCTGA ATTAAGGAAG
    GAGATGATGG CGATATGGCC TAATCTCTCT CAGAAGAACC TGGACCTGCT GGTGACTCCT
    CACAAGTCGA CAGACCTGAC AGTAGGAAAG ATCTACGCTG CCATGATGAT CATGGAGTAT
    TATCGGCAAA GTAAAGCCAA ACGGACACAA GCTCTCCATG ATGAGCAGAA TCGAACGCCA
    TTAATGTTTC AGCGTCTGGA GCCGCCATCT CCTAGCCAGG ATGTGGGACC AGGACTCACT
    GGCCTTCCTG ACACACAAAT GCACCCTGCA AATCATCTGC CTGTTGATGA AAGGATCCCA
    GAGAGCCAAT CATGGGTGAC AGCGCGAGCA CAGGAGATTT CTCAAAAAGC AGGCAACTGG
    AGTCCAGAGG GCCAACATCC AGATGACACC ACAGACAACA GAAGGCAGTC TCAGACAGTA
    GAGATGAGAG AGATGGGGCG GGACGGGTAC TCGGACACAG AGCAATATCT GCCGATGGAA
    GGCCATGGCA GGGCTGCTTC CATGCCTCGA CTCCCAGCAG ACAACCAACA GCCACGGAGG
    AAAGGGCGTC ACCGGGGAGA AAATCTCACT CCTATCACAG ATAACAGTCC TATGAAGCGC
    TCGGCTTCAT CTTTGGGACA CGGGCGTGCA GGACGCTCCA TGCGAGGTGA GGATTATGCC
    ATGGAGAGGG TTATCCCAGA AGAGGGTCAC AGGCATGGGC ATCGCCACCG AGACCGTAGT
    CACCGGGCGT CAGAACGCTC CCTGAGCCGA TACACTGATG CTGACACAGG TCTTGGAACT
    GACCTCAGCA CAACCACACA ATCAGGAGAC CTGCCATCCA AGGAGAGAGA CCGGGGCCGA
    GCCAAAGACC GCAAGCACCA TCATCATCAT CATCACCACC ACGGCTCTTT AGATAAGGAG
    CACTACGGTC ATGAGAGAGA CAGAGGAGAG TACGGCCACA GACAGTCCAG AGAGAGAGAC
    CGCCGCTGGT CCCGCTCACC CAGTGAGGGC AGAGAGTGTC TGACTCACAG ACAGGGCAGT
    AGTTCAGTAA GCGGCAGTCC AGTCCCGTCC ACCTCCGGGA CCAGCACCCC CCGCCGTGGC
    CGCCGTCAGC TCCCCCAAAC CCCTGCTACA CCCCGGCCAC ATGTCACCTA CTCACCTGTG
    GTCCGTAAGC CCATCAGCAG TACCCCCCCT CCTGGCCAGC AGAGCCGACT CCCTACCCCG
    ACCTCCCGGC GCTTCAGTCC GACTGGCCCC GAGCCCCCGC TTCCCCCTCA TCACCACCCG
    TCACCACCCC ACCACGGCTC TCCCCGCTCC GGCCGACATG CACACTGGGG CCCCGAGCCT
    GCAGAGAGCG TGGAGGGTGA CGGCTTCTAT GACGACCAAG ACTATGAGTT CAATCACCAC
    GAGCCTCCAT CCTACGAGCA AAGCCCTGTG CAGGGCAACC CTCACCCCCA TTCCCCTAGG
    ACTTCCCGCC ACAACACCCC TCCTCAGGGC CAAGCGCACC CACGGCGCAT GCCAAATGGC
    TACCGTTCGT CTTCGCCCTC TCCACACCAC CACGCGCCCG CTCACCCTGG AACTCATAAA
    CCTCCTCATC CCAGAGGGCC CAGAAAGGGC CTCCATGAAC CCTACAGTGA GACTGACGAG
    GATGACTGGT GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330117.1
    CDS1507..8541
    Translation

    Target ORF information:

    RefSeq Version XM_021474442.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X28, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474442.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCTAAGGA GCAGCAGAAG AACCACAAAG GTTGTAAGTC GGTATGGGAG
    CAGCGCACTA GTGAGTTAAG ACGACAGACC CTCGTGAACA GCAGAGAGGC TCTTTATAAT
    GAGTTGGACC CTGAAGACCG TTGGAAGGTC TCATACTCCC GTCACATACG TCCCGACATG
    AAAACTCACC TGGACCGGCC GTTAGTCGTG GACCCTCAGG AGAACCGCAA TAACAACACT
    AACAAAACCC GTCCGGGTGA CGGCCAACCT CAGCATTCCC ATCAGCTCCT ACACAAACAG
    CCCAACTTCA ATGAGGCCGA GACTGGAGGA GGTGGAGGGT CTGAGTCCTG GCCCGCACTG
    GAAAGAGGAG ACTCAACAGG GTCCCGCCGC AGTCTTTTGG GCTCTGAGCA CTATGAGGAG
    GGTAGAGAGT CCCACCGCAG ACACCAGCAC AGCCAGCACT GCAGTCAGGC CCGTCAACAT
    CGCAAAGCTG CAGAGGGCGG TGGAGAGCCT GGAGGGAGGA GACACAGGAG TAAGGCCAAG
    GAAAGAGGAC CTGAGTGTGA GCATTCAGAT GGAGGGGAGC GGAAACACAG ACGGCATCGG
    CATGAGGGTG AGGGGAGGAA AGAGAGAGGG GTACGACACC GAAACAGGAG GGATGGCCAT
    GCAAGTGGCC CGACTCTCTC TACAACCCGT CCCATTCAGA AGACTCTGTC CAGGCAGGAC
    AGTCAGTACA GCGAAGACCT GGACAATGCC ATGAACAACA AACTTGCTAC CCAGCCACAT
    GATAGTCTGC TTAATCTGGC CAACACTGCC CACTCTGCCG GCCTGGCACA TACTGGTACA
    GAGAGCAGCC TCATCCTCAC CAACCCTTCC TCTACCATTG CAAATGTTAC TAGCATCGGT
    CACCTGGGTA TGAAACCTGA GTACACTGCC GTGGACATTC CTCCCATGTT CCCCTCCAGT
    AATGCCATTC TACAGGTCAA TAAGAATGCC AACACGGAGC CTCTGCCAAA GAAGGAAGAC
    ACAAAGGGTG ACGATGATGA TGATGAGAAA GATGACGGAG GGCCTAAACC CATGCCACCC
    TACACCTCAA TGTTCATTCT GACCACCACC AACCCATTTC GGAGATTGTG TCACTATATC
    GTCACACTGA GATATTTTGA GATGTGTATT CTACTGGTTA TTGCTATGAG CAGTATTGCC
    CTGGCTGCTG AAGACCCTGT CTGGCCTGAA TCTCCCCGCA ACAATGTCCT ACGATACTTT
    GACTATGTGT TCACTGGTGT GTTCACCTTT GAGATGCTCA TTAAGATGGT AGATTTAGGT
    CTTGTCCTGC ATCAAGGCTC ATACTTTCGT GATCTGTGGA ACATCCTGGA CTTTATAGTG
    GTTAGTGGTG CCCTGGTGGC GTTCGCCTTC ACAGGAAGCA GCAAAGGGAA AGACATCAGT
    ACTATCAAGT CTCTACGTGT GCTTCGAGTA CTGCGGCCGC TGAAAACCAT CAAACGTCTG
    CCAAAACTGA AGGCTGTCTT TGACTGTGTG GTGAACTCTC TGAAGAATGT GTTGAACATC
    CTCATCGTCT ACATGCTCTT CATGTTCATC TTCGCTGTGG TGGCTGTGCA GCTCTTTAAG
    GGACGTTTCT TTTACTGCAC TGATGAATCC AAAGAGTTTG AGCGCGACTG CAGGGGCGAG
    TATCTGGTCT ATGAAAGAGA TAACGAAGTG AGGTCACAGA AGCGGGAATG GAAGAAATAC
    GATTTCCACT ACGACAACGT GCTCTGGGCC CTTCTCACCC TCTTCACCGT TTCTACGGGA
    GAGGGGTGGC CGCAAGTGCT AAAACATTCA GTAGATGCGA CCTACGAGAA TCAGGGCCCG
    AGCCCAGGTT ACCGGATGGA AATGTCCATC TTTTACGTGG TGTACTTCGT GGTCTTCCCC
    TTCTTCTTCG TCAACATCTT CGTGGCTCTT ATCATCATTA CTTTCCAGGA GCAAGGAGAC
    AAAATGATGG AGGACTATAG CCTGGAAAAG AATGAGAGAG CCTGCATAGA CTTTGCCATC
    AACGCTAAGC CGCTCACACG GCACATGCCC CAGAACAAGC AGACCTTTCA GTACCGCATG
    TGGGAGTTTG TGGTGTCTCC TCCATTTGAA TACACCATCA TGGCCTTGAT CGCGCTCAAC
    ACTATTGTCC TTATGATGAA GTATGATGGA GCATCGCTTA CTTATGAAGA TGTGCTAAAA
    TACCTGAACA TAGTGTTCAC CTCTCTATTC TCCATGGAGT GCATATTGAA GATCATAGCC
    TTCGGAGCAT TGAACTACTT CAAAGATGCC TGGAACATCT TCGACTGTGT GACTGTACTT
    GGCAGCATCA CTGATATTTT AGTCACAGAG CTGGGGAACA ACTTCATAAA TCTGAGTTTT
    CTAAGGCTCT TCAGAGCTGC TCGTCTCATT AAGTTGCTCA GGCAGGGTGA AACCATACGC
    ATCCTGCTCT GGACTTTTGT CCAGTCTTTC AAGGCTCTGC CGTATGTGTG CCTACTCATC
    GCCATGCTGT TCTTCATCTA CGCCATCATT GGCATGCAGC TGTTTGGGAA CATTAAGATC
    GAGGAGAACA GTGACAGTGC CATTACCCAG CACAACAACT TCAGGACTTT TTTCCAGGCC
    CTAATGCTGC TCTTTAGGAG TGCTACAGGT GAGGCATGGC ATGACATCAT GCTGTCATGT
    CTGGGAAAGA AGCCGTGTGA TATTCTCTCT GATAACCCGA AGCCAGAATG CGGGAGCGAG
    TTTGCCTACC TCTACTTTGT CTCCTTCATC TTCCTCTGTT CATTTCTGAT GCTGAATCTG
    TTCGTGGCTG TGATCATGGA TAATTTTGAA TACCTGACGC GTGACTCCTC TATCCTGGGA
    CCCCATCACC TGGACGAGTA TGTGAGGATC TGGGCAGAAT ATGATCCTGC TGCTTGCGGG
    CGCATTCATT ATAAGGATAT GTACAGTTTA TTGCGAGTTA TCGACCCCCC TCTGGGCTTA
    GGCAAGAAAT GTCCTCATAG GGTGGCCTGC AAGAGACTGT TGCGGATGGA TCTGCCGGTT
    GCCGATGACA ACACAGTGCA CTTTAATTCT ACCCTTATGG CCTTAATCCG AACCGCTCTG
    GACATCAAGA TCGCCAAGGG AGGTGTAGAT AAGCACCAGA TGGATGCTGA ATTAAGGAAG
    GAGATGATGG CGATATGGCC TAATCTCTCT CAGAAGAACC TGGACCTGCT GGTGACTCCT
    CACAAGTCGA CAGACCTGAC AGTAGGAAAG ATCTACGCTG CCATGATGAT CATGGAGTAT
    TATCGGCAAA GTAAAGCCAA ACGGACACAA GCTCTCCATG ATGAGCAGAA TCGAACGCCA
    TTAATGTTTC AGCGTCTGGA GCCGCCATCT CCTAGCCAGG ATGTGGGACC AGGACTCACT
    GGCCTTCCTG ACACACAAAT GCACCCTGCA AATCATCTGC CTGTTGATGA AAGGATCCCA
    GAGAGCCAAT CATGGGTGAC AGCGCGAGCA CAGGAGATTT CTCAAAAAGC AGGCAACTGG
    AGTCCAGAGG GCCAACATCC AGATGACACC ACAGACAACA GAAGGCAGTC TCAGACAGTA
    GAGATGAGAG AGATGGGGCG GGACGGGTAC TCGGACACAG AGCAATATCT GCCGATGGAA
    GGCCATGGCA GGGCTGCTTC CATGCCTCGA CTCCCAGCAG ACAACCAACA GCCACGGAGG
    AAAGGGCGTC ACCGGGGAGA AAATCTCACT CCTATCACAG ATAACAGTCC TATGAAGCGC
    TCGGCTTCAT CTTTGGGACA CGGGCGTGCA GGACGCTCCA TGCGAGGTGA GGATTATGCC
    ATGGAGAGGG TTATCCCAGA AGAGGGTCAC AGGCATGGGC ATCGCCACCG AGACCGTAGT
    CACCGGGCGT CAGAACGCTC CCTGAGCCGA TACACTGATG CTGACACAGG TCTTGGAACT
    GACCTCAGCA CAACCACACA ATCAGGAGAC CTGCCATCCA AGGAGAGAGA CCGGGGCCGA
    GCCAAAGACC GCAAGCACCA TCATCATCAT CATCACCACC ACGGCTCTTT AGATAAGGAG
    CACTACGGTC ATGAGAGAGA CAGAGGAGAG TACGGCCACA GACAGTCCAG AGAGAGAGAC
    CGCCGCTGGT CCCGCTCACC CAGTGAGGGC AGAGAGTGTC TGACTCACAG ACAGGGCAGT
    AGTTCAGTAA GCGGCAGTCC AGTCCCGTCC ACCTCCGGGA CCAGCACCCC CCGCCGTGGC
    CGCCGTCAGC TCCCCCAAAC CCCTGCTACA CCCCGGCCAC ATGTCACCTA CTCACCTGTG
    GTCCGTAAGC CCATCAGCAG TACCCCCCCT CCTGGCCAGC AGAGCCGACT CCCTACCCCG
    ACCTCCCGGC GCTTCAGTCC GACTGGCCCC GAGCCCCCGC TTCCCCCTCA TCACCACCCG
    TCACCACCCC ACCACGGCTC TCCCCGCTCC GGCCGACATG CACACTGGGG CCCCGAGCCT
    GCAGAGAGCG TGGAGGGTGA CGGCTTCTAT GACGACCAAG ACTATGAGTT CAATCACCAC
    GAGCCTCCAT CCTACGAGCA AAGCCCTGTG CAGGGCAACC CTCACCCCCA TTCCCCTAGG
    ACTTCCCGCC ACAACACCCC TCCTCAGGGC CAAGCGCACC CACGGCGCAT GCCAAATGGC
    TACCGTTCGT CTTCGCCCTC TCCACACCAC CACGCGCCCG CTCACCCTGG AACTCATAAA
    CCTCCTCATC CCAGAGGGCC CAGAAAGGGC CTCCATGAAC CCTACAGTGA GACTGACGAG
    GATGACTGGT GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43660
    Clone ID Related Accession (Same CDS sequence) XM_009299585.3 , XM_009299585.2
    Accession Version XM_009299585.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7074bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009299585.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG
    GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT
    GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC
    CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC
    ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT
    GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT
    GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT
    GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT
    CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA
    AGGCAGTCTC AGACAGTAGA GATGAGAGAG ATGGGGCGGG ACGGGTACTC GGACACAGAG
    CAATATCTGC CGATGGAAGG CCATGGCAGG GCTGCTTCCA TGCCTCGACT CCCAGCAGAC
    AACCAACAGC CTATCACAGA TAACAGTCCT ATGAAGCGCT CGGCTTCATC TTTGGGACAC
    GGGCGTGCAG GACGCTCCAT GCGAGGTGAG GATTATGCCA TGGAGAGGGT TATCCCAGAA
    GAGGGTCACA GGCATGGGCA TCGCCACCGA GACCGTAGTC ACCGGGCGTC AGAACGCTCC
    CTGAGCCGAT ACACTGATGC TGACACAGGT CTTGGAACTG ACCTCAGCAC AACCACACAA
    TCAGGAGACC TGCCATCCAA GGAGAGAGAC CGGGGCCGAG CCAAAGACCG CAAGCACCAT
    CATCATCATC ATCACCACCA CGGCTCTTTA GATAAGGAGC ACTACGGTCA TGAGAGAGAC
    AGAGGAGAGT ACGGCCACAG ACAGTCCAGA GAGAGAGACC GCCGCTGGTC CCGCTCACCC
    AGTGAGGGCA GAGAGTGTCT GACTCACAGA CAGGGCAGTA GTTCAGTAAG CGGCAGTCCA
    GTCCCGTCCA CCTCCGGGAC CAGCACCCCC CGCCGTGGCC GCCGTCAGCT CCCCCAAACC
    CCTGCTACAC CCCGGCCACA TGTCACCTAC TCACCTGTGG TCCGTAAGCC CATCAGCAGT
    ACCCCCCCTC CTGGCCAGCA GAGCCGACTC CCTACCCCGA CCTCCCGGCG CTTCAGTCCG
    ACTGGCCCCG AGCCCCCGCT TCCCCCTCAT CACCACCCGT CACCACCCCA CCACGGCTCT
    CCCCGCTCCG GCCGACATGC ACACTGGGGC CCCGAGCCTG CAGAGAGCGT GGAGGGTGAC
    GGCTTCTATG ACGACCAAGA CTATGAGTTC AATCACCACG AGCCTCCATC CTACGAGCAA
    AGCCCTGTGC AGGGCAACCC TCACCCCCAT TCCCCTAGGA CTTCCCGCCA CAACACCCCT
    CCTCAGGGCC AAGCGCACCC ACGGCGCATG CCAAATGGCT ACCGTTCGTC TTCGCCCTCT
    CCACACCACC ACGCGCCCGC TCACCCTGGA ACTCATAAAC CTCCTCATCC CAGAGGGCCC
    AGAAAGGGCC TCCATGAACC CTACAGTGAG ACTGACGAGG ATGACTGGTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009297860.1
    CDS1505..8578
    Translation

    Target ORF information:

    RefSeq Version XM_009299585.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009299585.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG
    GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT
    GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC
    CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC
    ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT
    GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT
    GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT
    GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT
    CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA
    AGGCAGTCTC AGACAGTAGA GATGAGAGAG ATGGGGCGGG ACGGGTACTC GGACACAGAG
    CAATATCTGC CGATGGAAGG CCATGGCAGG GCTGCTTCCA TGCCTCGACT CCCAGCAGAC
    AACCAACAGC CTATCACAGA TAACAGTCCT ATGAAGCGCT CGGCTTCATC TTTGGGACAC
    GGGCGTGCAG GACGCTCCAT GCGAGGTGAG GATTATGCCA TGGAGAGGGT TATCCCAGAA
    GAGGGTCACA GGCATGGGCA TCGCCACCGA GACCGTAGTC ACCGGGCGTC AGAACGCTCC
    CTGAGCCGAT ACACTGATGC TGACACAGGT CTTGGAACTG ACCTCAGCAC AACCACACAA
    TCAGGAGACC TGCCATCCAA GGAGAGAGAC CGGGGCCGAG CCAAAGACCG CAAGCACCAT
    CATCATCATC ATCACCACCA CGGCTCTTTA GATAAGGAGC ACTACGGTCA TGAGAGAGAC
    AGAGGAGAGT ACGGCCACAG ACAGTCCAGA GAGAGAGACC GCCGCTGGTC CCGCTCACCC
    AGTGAGGGCA GAGAGTGTCT GACTCACAGA CAGGGCAGTA GTTCAGTAAG CGGCAGTCCA
    GTCCCGTCCA CCTCCGGGAC CAGCACCCCC CGCCGTGGCC GCCGTCAGCT CCCCCAAACC
    CCTGCTACAC CCCGGCCACA TGTCACCTAC TCACCTGTGG TCCGTAAGCC CATCAGCAGT
    ACCCCCCCTC CTGGCCAGCA GAGCCGACTC CCTACCCCGA CCTCCCGGCG CTTCAGTCCG
    ACTGGCCCCG AGCCCCCGCT TCCCCCTCAT CACCACCCGT CACCACCCCA CCACGGCTCT
    CCCCGCTCCG GCCGACATGC ACACTGGGGC CCCGAGCCTG CAGAGAGCGT GGAGGGTGAC
    GGCTTCTATG ACGACCAAGA CTATGAGTTC AATCACCACG AGCCTCCATC CTACGAGCAA
    AGCCCTGTGC AGGGCAACCC TCACCCCCAT TCCCCTAGGA CTTCCCGCCA CAACACCCCT
    CCTCAGGGCC AAGCGCACCC ACGGCGCATG CCAAATGGCT ACCGTTCGTC TTCGCCCTCT
    CCACACCACC ACGCGCCCGC TCACCCTGGA ACTCATAAAC CTCCTCATCC CAGAGGGCCC
    AGAAAGGGCC TCCATGAACC CTACAGTGAG ACTGACGAGG ATGACTGGTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43660
    Clone ID Related Accession (Same CDS sequence) XM_009299585.3 , XM_009299585.2
    Accession Version XM_009299585.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7074bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299585.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG
    GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT
    GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC
    CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC
    ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT
    GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT
    GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT
    GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT
    CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA
    AGGCAGTCTC AGACAGTAGA GATGAGAGAG ATGGGGCGGG ACGGGTACTC GGACACAGAG
    CAATATCTGC CGATGGAAGG CCATGGCAGG GCTGCTTCCA TGCCTCGACT CCCAGCAGAC
    AACCAACAGC CTATCACAGA TAACAGTCCT ATGAAGCGCT CGGCTTCATC TTTGGGACAC
    GGGCGTGCAG GACGCTCCAT GCGAGGTGAG GATTATGCCA TGGAGAGGGT TATCCCAGAA
    GAGGGTCACA GGCATGGGCA TCGCCACCGA GACCGTAGTC ACCGGGCGTC AGAACGCTCC
    CTGAGCCGAT ACACTGATGC TGACACAGGT CTTGGAACTG ACCTCAGCAC AACCACACAA
    TCAGGAGACC TGCCATCCAA GGAGAGAGAC CGGGGCCGAG CCAAAGACCG CAAGCACCAT
    CATCATCATC ATCACCACCA CGGCTCTTTA GATAAGGAGC ACTACGGTCA TGAGAGAGAC
    AGAGGAGAGT ACGGCCACAG ACAGTCCAGA GAGAGAGACC GCCGCTGGTC CCGCTCACCC
    AGTGAGGGCA GAGAGTGTCT GACTCACAGA CAGGGCAGTA GTTCAGTAAG CGGCAGTCCA
    GTCCCGTCCA CCTCCGGGAC CAGCACCCCC CGCCGTGGCC GCCGTCAGCT CCCCCAAACC
    CCTGCTACAC CCCGGCCACA TGTCACCTAC TCACCTGTGG TCCGTAAGCC CATCAGCAGT
    ACCCCCCCTC CTGGCCAGCA GAGCCGACTC CCTACCCCGA CCTCCCGGCG CTTCAGTCCG
    ACTGGCCCCG AGCCCCCGCT TCCCCCTCAT CACCACCCGT CACCACCCCA CCACGGCTCT
    CCCCGCTCCG GCCGACATGC ACACTGGGGC CCCGAGCCTG CAGAGAGCGT GGAGGGTGAC
    GGCTTCTATG ACGACCAAGA CTATGAGTTC AATCACCACG AGCCTCCATC CTACGAGCAA
    AGCCCTGTGC AGGGCAACCC TCACCCCCAT TCCCCTAGGA CTTCCCGCCA CAACACCCCT
    CCTCAGGGCC AAGCGCACCC ACGGCGCATG CCAAATGGCT ACCGTTCGTC TTCGCCCTCT
    CCACACCACC ACGCGCCCGC TCACCCTGGA ACTCATAAAC CTCCTCATCC CAGAGGGCCC
    AGAAAGGGCC TCCATGAACC CTACAGTGAG ACTGACGAGG ATGACTGGTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009297860.1
    CDS1466..8539
    Translation

    Target ORF information:

    RefSeq Version XM_009299585.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009299585.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
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    3421
    3481
    3541
    3601
    3661
    3721
    3781
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    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG
    GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT
    GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC
    CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC
    ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT
    GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT
    GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT
    GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT
    CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA
    AGGCAGTCTC AGACAGTAGA GATGAGAGAG ATGGGGCGGG ACGGGTACTC GGACACAGAG
    CAATATCTGC CGATGGAAGG CCATGGCAGG GCTGCTTCCA TGCCTCGACT CCCAGCAGAC
    AACCAACAGC CTATCACAGA TAACAGTCCT ATGAAGCGCT CGGCTTCATC TTTGGGACAC
    GGGCGTGCAG GACGCTCCAT GCGAGGTGAG GATTATGCCA TGGAGAGGGT TATCCCAGAA
    GAGGGTCACA GGCATGGGCA TCGCCACCGA GACCGTAGTC ACCGGGCGTC AGAACGCTCC
    CTGAGCCGAT ACACTGATGC TGACACAGGT CTTGGAACTG ACCTCAGCAC AACCACACAA
    TCAGGAGACC TGCCATCCAA GGAGAGAGAC CGGGGCCGAG CCAAAGACCG CAAGCACCAT
    CATCATCATC ATCACCACCA CGGCTCTTTA GATAAGGAGC ACTACGGTCA TGAGAGAGAC
    AGAGGAGAGT ACGGCCACAG ACAGTCCAGA GAGAGAGACC GCCGCTGGTC CCGCTCACCC
    AGTGAGGGCA GAGAGTGTCT GACTCACAGA CAGGGCAGTA GTTCAGTAAG CGGCAGTCCA
    GTCCCGTCCA CCTCCGGGAC CAGCACCCCC CGCCGTGGCC GCCGTCAGCT CCCCCAAACC
    CCTGCTACAC CCCGGCCACA TGTCACCTAC TCACCTGTGG TCCGTAAGCC CATCAGCAGT
    ACCCCCCCTC CTGGCCAGCA GAGCCGACTC CCTACCCCGA CCTCCCGGCG CTTCAGTCCG
    ACTGGCCCCG AGCCCCCGCT TCCCCCTCAT CACCACCCGT CACCACCCCA CCACGGCTCT
    CCCCGCTCCG GCCGACATGC ACACTGGGGC CCCGAGCCTG CAGAGAGCGT GGAGGGTGAC
    GGCTTCTATG ACGACCAAGA CTATGAGTTC AATCACCACG AGCCTCCATC CTACGAGCAA
    AGCCCTGTGC AGGGCAACCC TCACCCCCAT TCCCCTAGGA CTTCCCGCCA CAACACCCCT
    CCTCAGGGCC AAGCGCACCC ACGGCGCATG CCAAATGGCT ACCGTTCGTC TTCGCCCTCT
    CCACACCACC ACGCGCCCGC TCACCCTGGA ACTCATAAAC CTCCTCATCC CAGAGGGCCC
    AGAAAGGGCC TCCATGAACC CTACAGTGAG ACTGACGAGG ATGACTGGTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43661
    Clone ID Related Accession (Same CDS sequence) XM_009299586.2
    Accession Version XM_009299586.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7059bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X10
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299586.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA ACAACTTCAT AAATCTGAGT
    TTTCTAAGGC TCTTCAGAGC TGCTCGTCTC ATTAAGTTGC TCAGGCAGGG TGAAACCATA
    CGCATCCTGC TCTGGACTTT TGTCCAGTCT TTCAAGGCTC TGCCGTATGT GTGCCTACTC
    ATCGCCATGC TGTTCTTCAT CTACGCCATC ATTGGCATGC AGCTGTTTGG GAACATTAAG
    ATCGAGGAGA ACAGTGACAG TGCCATTACC CAGCACAACA ACTTCAGGAC TTTTTTCCAG
    GCCCTAATGC TGCTCTTTAG GAGTGCTACA GGTGAGGCAT GGCATGACAT CATGCTGTCA
    TGTCTGGGAA AGAAGCCGTG TGATATTCTC TCTGATAACC CGAAGCCAGA ATGCGGGAGC
    GAGTTTGCCT ACCTCTACTT TGTCTCCTTC ATCTTCCTCT GTTCATTTCT GATGCTGAAT
    CTGTTCGTGG CTGTGATCAT GGATAATTTT GAATACCTGA CGCGTGACTC CTCTATCCTG
    GGACCCCATC ACCTGGACGA GTATGTGAGG ATCTGGGCAG AATATGATCC TGCTGCTTGT
    GGCAGAATCA GTTACAGGGA TATGTATGAG ATGCTCAGGC ACATGTCTCC TCCGTTAGGC
    CTGGGGAAGA AGTGCCCTGC ACGGGTTGCC TACAAGAGAC TGTTGCGGAT GGATCTGCCG
    GTTGCCGATG ACAACACAGT GCACTTTAAT TCTACCCTTA TGGCCTTAAT CCGAACCGCT
    CTGGACATCA AGATCGCCAA GGGAGGTGTA GATAAGCACC AGATGGATGC TGAATTAAGG
    AAGGAGATGA TGGCGATATG GCCTAATCTC TCTCAGAAGA ACCTGGACCT GCTGGTGACT
    CCTCACAAGT CGACAGACCT GACAGTAGGA AAGATCTACG CTGCCATGAT GATCATGGAG
    TATTATCGGC AAAGTAAAGC CAAACGGACA CAAGCTCTCC ATGATGAGCA GAATCGAACG
    CCATTAATGT TTCAGCGTCT GGAGCCGCCA TCTCCTAGCC AGGATGTGGG ACCAGGACTC
    ACTGGCCTTC CTGACACACA AATGCACCCT GCAAATCATC TGCCTGTTGA TGAAAGGATC
    CCAGAGAGCC AATCATGGGT GACAGCGCGA GCACAGGAGA TTTCTCAAAA AGCAGGCAAC
    TGGAGTCCAG AGGGCCAACA TCCAGATGAC ACCACAGACA ACAGAAGGCA GTCTCAGACA
    GTAGAGATGA GAGAGATGGG GCGGGACGGG TACTCGGACA CAGAGCAATA TCTGCCGATG
    GAAGGCCATG GCAGGGCTGC TTCCATGCCT CGACTCCCAG CAGACAACCA ACAGCCTATC
    ACAGATAACA GTCCTATGAA GCGCTCGGCT TCATCTTTGG GACACGGGCG TGCAGGACGC
    TCCATGCGAG GTGAGGATTA TGCCATGGAG AGGGTTATCC CAGAAGAGGG TCACAGGCAT
    GGGCATCGCC ACCGAGACCG TAGTCACCGG GCGTCAGAAC GCTCCCTGAG CCGATACACT
    GATGCTGACA CAGGTCTTGG AACTGACCTC AGCACAACCA CACAATCAGG AGACCTGCCA
    TCCAAGGAGA GAGACCGGGG CCGAGCCAAA GACCGCAAGC ACCATCATCA TCATCATCAC
    CACCACGGCT CTTTAGATAA GGAGCACTAC GGTCATGAGA GAGACAGAGG AGAGTACGGC
    CACAGACAGT CCAGAGAGAG AGACCGCCGC TGGTCCCGCT CACCCAGTGA GGGCAGAGAG
    TGTCTGACTC ACAGACAGGG CAGTAGTTCA GTAAGCGGCA GTCCAGTCCC GTCCACCTCC
    GGGACCAGCA CCCCCCGCCG TGGCCGCCGT CAGCTCCCCC AAACCCCTGC TACACCCCGG
    CCACATGTCA CCTACTCACC TGTGGTCCGT AAGCCCATCA GCAGTACCCC CCCTCCTGGC
    CAGCAGAGCC GACTCCCTAC CCCGACCTCC CGGCGCTTCA GTCCGACTGG CCCCGAGCCC
    CCGCTTCCCC CTCATCACCA CCCGTCACCA CCCCACCACG GCTCTCCCCG CTCCGGCCGA
    CATGCACACT GGGGCCCCGA GCCTGCAGAG AGCGTGGAGG GTGACGGCTT CTATGACGAC
    CAAGACTATG AGTTCAATCA CCACGAGCCT CCATCCTACG AGCAAAGCCC TGTGCAGGGC
    AACCCTCACC CCCATTCCCC TAGGACTTCC CGCCACAACA CCCCTCCTCA GGGCCAAGCG
    CACCCACGGC GCATGCCAAA TGGCTACCGT TCGTCTTCGC CCTCTCCACA CCACCACGCG
    CCCGCTCACC CTGGAACTCA TAAACCTCCT CATCCCAGAG GGCCCAGAAA GGGCCTCCAT
    GAACCCTACA GTGAGACTGA CGAGGATGAC TGGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009297861.1
    CDS1468..8526
    Translation

    Target ORF information:

    RefSeq Version XM_009299586.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009299586.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA ACAACTTCAT AAATCTGAGT
    TTTCTAAGGC TCTTCAGAGC TGCTCGTCTC ATTAAGTTGC TCAGGCAGGG TGAAACCATA
    CGCATCCTGC TCTGGACTTT TGTCCAGTCT TTCAAGGCTC TGCCGTATGT GTGCCTACTC
    ATCGCCATGC TGTTCTTCAT CTACGCCATC ATTGGCATGC AGCTGTTTGG GAACATTAAG
    ATCGAGGAGA ACAGTGACAG TGCCATTACC CAGCACAACA ACTTCAGGAC TTTTTTCCAG
    GCCCTAATGC TGCTCTTTAG GAGTGCTACA GGTGAGGCAT GGCATGACAT CATGCTGTCA
    TGTCTGGGAA AGAAGCCGTG TGATATTCTC TCTGATAACC CGAAGCCAGA ATGCGGGAGC
    GAGTTTGCCT ACCTCTACTT TGTCTCCTTC ATCTTCCTCT GTTCATTTCT GATGCTGAAT
    CTGTTCGTGG CTGTGATCAT GGATAATTTT GAATACCTGA CGCGTGACTC CTCTATCCTG
    GGACCCCATC ACCTGGACGA GTATGTGAGG ATCTGGGCAG AATATGATCC TGCTGCTTGT
    GGCAGAATCA GTTACAGGGA TATGTATGAG ATGCTCAGGC ACATGTCTCC TCCGTTAGGC
    CTGGGGAAGA AGTGCCCTGC ACGGGTTGCC TACAAGAGAC TGTTGCGGAT GGATCTGCCG
    GTTGCCGATG ACAACACAGT GCACTTTAAT TCTACCCTTA TGGCCTTAAT CCGAACCGCT
    CTGGACATCA AGATCGCCAA GGGAGGTGTA GATAAGCACC AGATGGATGC TGAATTAAGG
    AAGGAGATGA TGGCGATATG GCCTAATCTC TCTCAGAAGA ACCTGGACCT GCTGGTGACT
    CCTCACAAGT CGACAGACCT GACAGTAGGA AAGATCTACG CTGCCATGAT GATCATGGAG
    TATTATCGGC AAAGTAAAGC CAAACGGACA CAAGCTCTCC ATGATGAGCA GAATCGAACG
    CCATTAATGT TTCAGCGTCT GGAGCCGCCA TCTCCTAGCC AGGATGTGGG ACCAGGACTC
    ACTGGCCTTC CTGACACACA AATGCACCCT GCAAATCATC TGCCTGTTGA TGAAAGGATC
    CCAGAGAGCC AATCATGGGT GACAGCGCGA GCACAGGAGA TTTCTCAAAA AGCAGGCAAC
    TGGAGTCCAG AGGGCCAACA TCCAGATGAC ACCACAGACA ACAGAAGGCA GTCTCAGACA
    GTAGAGATGA GAGAGATGGG GCGGGACGGG TACTCGGACA CAGAGCAATA TCTGCCGATG
    GAAGGCCATG GCAGGGCTGC TTCCATGCCT CGACTCCCAG CAGACAACCA ACAGCCTATC
    ACAGATAACA GTCCTATGAA GCGCTCGGCT TCATCTTTGG GACACGGGCG TGCAGGACGC
    TCCATGCGAG GTGAGGATTA TGCCATGGAG AGGGTTATCC CAGAAGAGGG TCACAGGCAT
    GGGCATCGCC ACCGAGACCG TAGTCACCGG GCGTCAGAAC GCTCCCTGAG CCGATACACT
    GATGCTGACA CAGGTCTTGG AACTGACCTC AGCACAACCA CACAATCAGG AGACCTGCCA
    TCCAAGGAGA GAGACCGGGG CCGAGCCAAA GACCGCAAGC ACCATCATCA TCATCATCAC
    CACCACGGCT CTTTAGATAA GGAGCACTAC GGTCATGAGA GAGACAGAGG AGAGTACGGC
    CACAGACAGT CCAGAGAGAG AGACCGCCGC TGGTCCCGCT CACCCAGTGA GGGCAGAGAG
    TGTCTGACTC ACAGACAGGG CAGTAGTTCA GTAAGCGGCA GTCCAGTCCC GTCCACCTCC
    GGGACCAGCA CCCCCCGCCG TGGCCGCCGT CAGCTCCCCC AAACCCCTGC TACACCCCGG
    CCACATGTCA CCTACTCACC TGTGGTCCGT AAGCCCATCA GCAGTACCCC CCCTCCTGGC
    CAGCAGAGCC GACTCCCTAC CCCGACCTCC CGGCGCTTCA GTCCGACTGG CCCCGAGCCC
    CCGCTTCCCC CTCATCACCA CCCGTCACCA CCCCACCACG GCTCTCCCCG CTCCGGCCGA
    CATGCACACT GGGGCCCCGA GCCTGCAGAG AGCGTGGAGG GTGACGGCTT CTATGACGAC
    CAAGACTATG AGTTCAATCA CCACGAGCCT CCATCCTACG AGCAAAGCCC TGTGCAGGGC
    AACCCTCACC CCCATTCCCC TAGGACTTCC CGCCACAACA CCCCTCCTCA GGGCCAAGCG
    CACCCACGGC GCATGCCAAA TGGCTACCGT TCGTCTTCGC CCTCTCCACA CCACCACGCG
    CCCGCTCACC CTGGAACTCA TAAACCTCCT CATCCCAGAG GGCCCAGAAA GGGCCTCCAT
    GAACCCTACA GTGAGACTGA CGAGGATGAC TGGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43662
    Clone ID Related Accession (Same CDS sequence) XM_009299587.2
    Accession Version XM_009299587.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7047bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X12
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299587.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACAGGAAGC AGCAAAGGGA AAGACATCAG TACTATCAAG
    TCTCTACGTG TGCTTCGAGT ACTGCGGCCG CTGAAAACCA TCAAACGTCT GCCAAAACTG
    AAGGCTGTCT TTGACTGTGT GGTGAACTCT CTGAAGAATG TGTTGAACAT CCTCATCGTC
    TACATGCTCT TCATGTTCAT CTTCGCTGTG GTGGCTGTGC AGCTCTTTAA GGGACGTTTC
    TTTTACTGCA CTGATGAATC CAAAGAGTTT GAGCGCGACT GCAGGGGCGA GTATCTGGTC
    TATGAAAGAG ATAACGAAGT GAGGTCACAG AAGCGGGAAT GGAAGAAATA CGATTTCCAC
    TACGACAACG TGCTCTGGGC CCTTCTCACC CTCTTCACCG TTTCTACGGG AGAGGGGTGG
    CCGCAAGTGC TAAAACATTC AGTAGATGCG ACCTACGAGA ATCAGGGCCC GAGCCCAGGT
    TACCGGATGG AAATGTCCAT CTTTTACGTG GTGTACTTCG TGGTCTTCCC CTTCTTCTTC
    GTCAACATCT TCGTGGCTCT TATCATCATT ACTTTCCAGG AGCAAGGAGA CAAAATGATG
    GAGGACTATA GCCTGGAAAA GAATGAGAGA GCCTGCATAG ACTTTGCCAT CAACGCTAAG
    CCGCTCACAC GGCACATGCC CCAGAACAAG CAGACCTTTC AGTACCGCAT GTGGGAGTTT
    GTGGTGTCTC CTCCATTTGA ATACACCATC ATGGCCTTGA TCGCGCTCAA CACTATTGTC
    CTTATGATGA AGTATGATGG AGCATCGCTT ACTTATGAAG ATGTGCTAAA ATACCTGAAC
    ATAGTGTTCA CCTCTCTATT CTCCATGGAG TGCATATTGA AGATCATAGC CTTCGGAGCA
    TTGAACTACT TCAAAGATGC CTGGAACATC TTCGACTGTG TGACTGTACT TGGCAGCATC
    ACTGATATTT TAGTCACAGA GCTGGGGAAC AACTTCATAA ATCTGAGTTT TCTAAGGCTC
    TTCAGAGCTG CTCGTCTCAT TAAGTTGCTC AGGCAGGGTG AAACCATACG CATCCTGCTC
    TGGACTTTTG TCCAGTCTTT CAAGGCTCTG CCGTATGTGT GCCTACTCAT CGCCATGCTG
    TTCTTCATCT ACGCCATCAT TGGCATGCAG CTGTTTGGGA ACATTAAGAT CGAGGAGAAC
    AGTGACAGTG CCATTACCCA GCACAACAAC TTCAGGACTT TTTTCCAGGC CCTAATGCTG
    CTCTTTAGGA GTGCTACAGG TGAGGCATGG CATGACATCA TGCTGTCATG TCTGGGAAAG
    AAGCCGTGTG ATATTCTCTC TGATAACCCG AAGCCAGAAT GCGGGAGCGA GTTTGCCTAC
    CTCTACTTTG TCTCCTTCAT CTTCCTCTGT TCATTTCTGA TGCTGAATCT GTTCGTGGCT
    GTGATCATGG ATAATTTTGA ATACCTGACG CGTGACTCCT CTATCCTGGG ACCCCATCAC
    CTGGACGAGT ATGTGAGGAT CTGGGCAGAA TATGATCCTG CTGCTTGCGG GCGCATTCAT
    TATAAGGATA TGTACAGTTT ATTGCGAGTT ATCGACCCCC CTCTGGGCTT AGGCAAGAAA
    TGTCCTCATA GGGTGGCCTG CAAGAGACTG TTGCGGATGG ATCTGCCGGT TGCCGATGAC
    AACACAGTGC ACTTTAATTC TACCCTTATG GCCTTAATCC GAACCGCTCT GGACATCAAG
    ATCGCCAAGG GAGGTGTAGA TAAGCACCAG ATGGATGCTG AATTAAGGAA GGAGATGATG
    GCGATATGGC CTAATCTCTC TCAGAAGAAC CTGGACCTGC TGGTGACTCC TCACAAGTCG
    ACAGACCTGA CAGTAGGAAA GATCTACGCT GCCATGATGA TCATGGAGTA TTATCGGCAA
    AGTAAAGCCA AACGGACACA AGCTCTCCAT GATGAGCAGA ATCGAACGCC ATTAATGTTT
    CAGCGTCTGG AGCCGCCATC TCCTAGCCAG GATGTGGGAC CAGGACTCAC TGGCCTTCCT
    GACACACAAA TGCACCCTGC AAATCATCTG CCTGTTGATG AAAGGATCCC AGAGAGCCAA
    TCATGGGTGA CAGCGCGAGC ACAGGAGATT TCTCAAAAAG CAGGCAACTG GAGTCCAGAG
    GGCCAACATC CAGATGACAC CACAGACAAC AGAAGGCAGT CTCAGACAGT AGAGATGAGA
    GAGATGGGGC GGGACGGGTA CTCGGACACA GAGCAATATC TGCCGATGGA AGGCCATGGC
    AGGGCTGCTT CCATGCCTCG ACTCCCAGCA GACAACCAAC AGCCTATCAC AGATAACAGT
    CCTATGAAGC GCTCGGCTTC ATCTTTGGGA CACGGGCGTG CAGGACGCTC CATGCGAGGT
    GAGGATTATG CCATGGAGAG GGTTATCCCA GAAGAGGGTC ACAGGCATGG GCATCGCCAC
    CGAGACCGTA GTCACCGGGC GTCAGAACGC TCCCTGAGCC GATACACTGA TGCTGACACA
    GGTCTTGGAA CTGACCTCAG CACAACCACA CAATCAGGAG ACCTGCCATC CAAGGAGAGA
    GACCGGGGCC GAGCCAAAGA CCGCAAGCAC CATCATCATC ATCATCACCA CCACGGCTCT
    TTAGATAAGG AGCACTACGG TCATGAGAGA GACAGAGGAG AGTACGGCCA CAGACAGTCC
    AGAGAGAGAG ACCGCCGCTG GTCCCGCTCA CCCAGTGAGG GCAGAGAGTG TCTGACTCAC
    AGACAGGGCA GTAGTTCAGT AAGCGGCAGT CCAGTCCCGT CCACCTCCGG GACCAGCACC
    CCCCGCCGTG GCCGCCGTCA GCTCCCCCAA ACCCCTGCTA CACCCCGGCC ACATGTCACC
    TACTCACCTG TGGTCCGTAA GCCCATCAGC AGTACCCCCC CTCCTGGCCA GCAGAGCCGA
    CTCCCTACCC CGACCTCCCG GCGCTTCAGT CCGACTGGCC CCGAGCCCCC GCTTCCCCCT
    CATCACCACC CGTCACCACC CCACCACGGC TCTCCCCGCT CCGGCCGACA TGCACACTGG
    GGCCCCGAGC CTGCAGAGAG CGTGGAGGGT GACGGCTTCT ATGACGACCA AGACTATGAG
    TTCAATCACC ACGAGCCTCC ATCCTACGAG CAAAGCCCTG TGCAGGGCAA CCCTCACCCC
    CATTCCCCTA GGACTTCCCG CCACAACACC CCTCCTCAGG GCCAAGCGCA CCCACGGCGC
    ATGCCAAATG GCTACCGTTC GTCTTCGCCC TCTCCACACC ACCACGCGCC CGCTCACCCT
    GGAACTCATA AACCTCCTCA TCCCAGAGGG CCCAGAAAGG GCCTCCATGA ACCCTACAGT
    GAGACTGACG AGGATGACTG GTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009297862.1
    CDS1467..8513
    Translation

    Target ORF information:

    RefSeq Version XM_009299587.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009299587.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACAGGAAGC AGCAAAGGGA AAGACATCAG TACTATCAAG
    TCTCTACGTG TGCTTCGAGT ACTGCGGCCG CTGAAAACCA TCAAACGTCT GCCAAAACTG
    AAGGCTGTCT TTGACTGTGT GGTGAACTCT CTGAAGAATG TGTTGAACAT CCTCATCGTC
    TACATGCTCT TCATGTTCAT CTTCGCTGTG GTGGCTGTGC AGCTCTTTAA GGGACGTTTC
    TTTTACTGCA CTGATGAATC CAAAGAGTTT GAGCGCGACT GCAGGGGCGA GTATCTGGTC
    TATGAAAGAG ATAACGAAGT GAGGTCACAG AAGCGGGAAT GGAAGAAATA CGATTTCCAC
    TACGACAACG TGCTCTGGGC CCTTCTCACC CTCTTCACCG TTTCTACGGG AGAGGGGTGG
    CCGCAAGTGC TAAAACATTC AGTAGATGCG ACCTACGAGA ATCAGGGCCC GAGCCCAGGT
    TACCGGATGG AAATGTCCAT CTTTTACGTG GTGTACTTCG TGGTCTTCCC CTTCTTCTTC
    GTCAACATCT TCGTGGCTCT TATCATCATT ACTTTCCAGG AGCAAGGAGA CAAAATGATG
    GAGGACTATA GCCTGGAAAA GAATGAGAGA GCCTGCATAG ACTTTGCCAT CAACGCTAAG
    CCGCTCACAC GGCACATGCC CCAGAACAAG CAGACCTTTC AGTACCGCAT GTGGGAGTTT
    GTGGTGTCTC CTCCATTTGA ATACACCATC ATGGCCTTGA TCGCGCTCAA CACTATTGTC
    CTTATGATGA AGTATGATGG AGCATCGCTT ACTTATGAAG ATGTGCTAAA ATACCTGAAC
    ATAGTGTTCA CCTCTCTATT CTCCATGGAG TGCATATTGA AGATCATAGC CTTCGGAGCA
    TTGAACTACT TCAAAGATGC CTGGAACATC TTCGACTGTG TGACTGTACT TGGCAGCATC
    ACTGATATTT TAGTCACAGA GCTGGGGAAC AACTTCATAA ATCTGAGTTT TCTAAGGCTC
    TTCAGAGCTG CTCGTCTCAT TAAGTTGCTC AGGCAGGGTG AAACCATACG CATCCTGCTC
    TGGACTTTTG TCCAGTCTTT CAAGGCTCTG CCGTATGTGT GCCTACTCAT CGCCATGCTG
    TTCTTCATCT ACGCCATCAT TGGCATGCAG CTGTTTGGGA ACATTAAGAT CGAGGAGAAC
    AGTGACAGTG CCATTACCCA GCACAACAAC TTCAGGACTT TTTTCCAGGC CCTAATGCTG
    CTCTTTAGGA GTGCTACAGG TGAGGCATGG CATGACATCA TGCTGTCATG TCTGGGAAAG
    AAGCCGTGTG ATATTCTCTC TGATAACCCG AAGCCAGAAT GCGGGAGCGA GTTTGCCTAC
    CTCTACTTTG TCTCCTTCAT CTTCCTCTGT TCATTTCTGA TGCTGAATCT GTTCGTGGCT
    GTGATCATGG ATAATTTTGA ATACCTGACG CGTGACTCCT CTATCCTGGG ACCCCATCAC
    CTGGACGAGT ATGTGAGGAT CTGGGCAGAA TATGATCCTG CTGCTTGCGG GCGCATTCAT
    TATAAGGATA TGTACAGTTT ATTGCGAGTT ATCGACCCCC CTCTGGGCTT AGGCAAGAAA
    TGTCCTCATA GGGTGGCCTG CAAGAGACTG TTGCGGATGG ATCTGCCGGT TGCCGATGAC
    AACACAGTGC ACTTTAATTC TACCCTTATG GCCTTAATCC GAACCGCTCT GGACATCAAG
    ATCGCCAAGG GAGGTGTAGA TAAGCACCAG ATGGATGCTG AATTAAGGAA GGAGATGATG
    GCGATATGGC CTAATCTCTC TCAGAAGAAC CTGGACCTGC TGGTGACTCC TCACAAGTCG
    ACAGACCTGA CAGTAGGAAA GATCTACGCT GCCATGATGA TCATGGAGTA TTATCGGCAA
    AGTAAAGCCA AACGGACACA AGCTCTCCAT GATGAGCAGA ATCGAACGCC ATTAATGTTT
    CAGCGTCTGG AGCCGCCATC TCCTAGCCAG GATGTGGGAC CAGGACTCAC TGGCCTTCCT
    GACACACAAA TGCACCCTGC AAATCATCTG CCTGTTGATG AAAGGATCCC AGAGAGCCAA
    TCATGGGTGA CAGCGCGAGC ACAGGAGATT TCTCAAAAAG CAGGCAACTG GAGTCCAGAG
    GGCCAACATC CAGATGACAC CACAGACAAC AGAAGGCAGT CTCAGACAGT AGAGATGAGA
    GAGATGGGGC GGGACGGGTA CTCGGACACA GAGCAATATC TGCCGATGGA AGGCCATGGC
    AGGGCTGCTT CCATGCCTCG ACTCCCAGCA GACAACCAAC AGCCTATCAC AGATAACAGT
    CCTATGAAGC GCTCGGCTTC ATCTTTGGGA CACGGGCGTG CAGGACGCTC CATGCGAGGT
    GAGGATTATG CCATGGAGAG GGTTATCCCA GAAGAGGGTC ACAGGCATGG GCATCGCCAC
    CGAGACCGTA GTCACCGGGC GTCAGAACGC TCCCTGAGCC GATACACTGA TGCTGACACA
    GGTCTTGGAA CTGACCTCAG CACAACCACA CAATCAGGAG ACCTGCCATC CAAGGAGAGA
    GACCGGGGCC GAGCCAAAGA CCGCAAGCAC CATCATCATC ATCATCACCA CCACGGCTCT
    TTAGATAAGG AGCACTACGG TCATGAGAGA GACAGAGGAG AGTACGGCCA CAGACAGTCC
    AGAGAGAGAG ACCGCCGCTG GTCCCGCTCA CCCAGTGAGG GCAGAGAGTG TCTGACTCAC
    AGACAGGGCA GTAGTTCAGT AAGCGGCAGT CCAGTCCCGT CCACCTCCGG GACCAGCACC
    CCCCGCCGTG GCCGCCGTCA GCTCCCCCAA ACCCCTGCTA CACCCCGGCC ACATGTCACC
    TACTCACCTG TGGTCCGTAA GCCCATCAGC AGTACCCCCC CTCCTGGCCA GCAGAGCCGA
    CTCCCTACCC CGACCTCCCG GCGCTTCAGT CCGACTGGCC CCGAGCCCCC GCTTCCCCCT
    CATCACCACC CGTCACCACC CCACCACGGC TCTCCCCGCT CCGGCCGACA TGCACACTGG
    GGCCCCGAGC CTGCAGAGAG CGTGGAGGGT GACGGCTTCT ATGACGACCA AGACTATGAG
    TTCAATCACC ACGAGCCTCC ATCCTACGAG CAAAGCCCTG TGCAGGGCAA CCCTCACCCC
    CATTCCCCTA GGACTTCCCG CCACAACACC CCTCCTCAGG GCCAAGCGCA CCCACGGCGC
    ATGCCAAATG GCTACCGTTC GTCTTCGCCC TCTCCACACC ACCACGCGCC CGCTCACCCT
    GGAACTCATA AACCTCCTCA TCCCAGAGGG CCCAGAAAGG GCCTCCATGA ACCCTACAGT
    GAGACTGACG AGGATGACTG GTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43663
    Clone ID Related Accession (Same CDS sequence) XM_009299588.2
    Accession Version XM_009299588.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7047bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X13
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299588.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACAGGAAGC AGCAAAGGGA AAGACATCAG TACTATCAAG
    TCTCTACGTG TGCTTCGAGT ACTGCGGCCG CTGAAAACCA TCAAACGTCT GCCAAAACTG
    AAGGCTGTCT TTGACTGTGT GGTGAACTCT CTGAAGAATG TGTTGAACAT CCTCATCGTC
    TACATGCTCT TCATGTTCAT CTTCGCTGTG GTGGCTGTGC AGCTCTTTAA GGGACGTTTC
    TTTTACTGCA CTGATGAATC CAAAGAGTTT GAGCGCGACT GCAGGGGCGA GTATCTGGTC
    TATGAAAGAG ATAACGAAGT GAGGTCACAG AAGCGGGAAT GGAAGAAATA CGATTTCCAC
    TACGACAACG TGCTCTGGGC CCTTCTCACC CTCTTCACCG TTTCTACGGG AGAGGGGTGG
    CCGCAAGTGC TAAAACATTC AGTAGATGCG ACCTACGAGA ATCAGGGCCC GAGCCCAGGT
    TACCGGATGG AAATGTCCAT CTTTTACGTG GTGTACTTCG TGGTCTTCCC CTTCTTCTTC
    GTCAACATCT TCGTGGCTCT TATCATCATT ACTTTCCAGG AGCAAGGAGA CAAAATGATG
    GAGGACTATA GCCTGGAAAA GAATGAGAGA GCCTGCATAG ACTTTGCCAT CAACGCTAAG
    CCGCTCACAC GGCACATGCC CCAGAACAAG CAGACCTTTC AGTACCGCAT GTGGGAGTTT
    GTGGTGTCTC CTCCATTTGA ATACACCATC ATGGCCTTGA TCGCGCTCAA CACTATTGTC
    CTTATGATGA AGTATGATGG AGCATCGCTT ACTTATGAAG ATGTGCTAAA ATACCTGAAC
    ATAGTGTTCA CCTCTCTATT CTCCATGGAG TGCATATTGA AGATCATAGC CTTCGGAGCA
    TTGAACTACT TCAAAGATGC CTGGAACATC TTCGACTGTG TGACTGTACT TGGCAGCATC
    ACTGATATTT TAGTCACAGA GCTGGGGAAC AACTTCATAA ATCTGAGTTT TCTAAGGCTC
    TTCAGAGCTG CTCGTCTCAT TAAGTTGCTC AGGCAGGGTG AAACCATACG CATCCTGCTC
    TGGACTTTTG TCCAGTCTTT CAAGGCTCTG CCGTATGTGT GCCTACTCAT CGCCATGCTG
    TTCTTCATCT ACGCCATCAT TGGCATGCAG CTGTTTGGGA ACATTAAGAT CGAGGAGAAC
    AGTGACAGTG CCATTACCCA GCACAACAAC TTCAGGACTT TTTTCCAGGC CCTAATGCTG
    CTCTTTAGGA GTGCTACAGG TGAGGCATGG CATGACATCA TGCTGTCATG TCTGGGAAAG
    AAGCCGTGTG ATATTCTCTC TGATAACCCG AAGCCAGAAT GCGGGAGCGA GTTTGCCTAC
    CTCTACTTTG TCTCCTTCAT CTTCCTCTGT TCATTTCTGA TGCTGAATCT GTTCGTGGCT
    GTGATCATGG ATAATTTTGA ATACCTGACG CGTGACTCCT CTATCCTGGG ACCCCATCAC
    CTGGACGAGT ATGTGAGGAT CTGGGCAGAA TATGATCCTG CTGCTTGTGG CAGAATCAGT
    TACAGGGATA TGTATGAGAT GCTCAGGCAC ATGTCTCCTC CGTTAGGCCT GGGGAAGAAG
    TGCCCTGCAC GGGTTGCCTA CAAGAGACTG TTGCGGATGG ATCTGCCGGT TGCCGATGAC
    AACACAGTGC ACTTTAATTC TACCCTTATG GCCTTAATCC GAACCGCTCT GGACATCAAG
    ATCGCCAAGG GAGGTGTAGA TAAGCACCAG ATGGATGCTG AATTAAGGAA GGAGATGATG
    GCGATATGGC CTAATCTCTC TCAGAAGAAC CTGGACCTGC TGGTGACTCC TCACAAGTCG
    ACAGACCTGA CAGTAGGAAA GATCTACGCT GCCATGATGA TCATGGAGTA TTATCGGCAA
    AGTAAAGCCA AACGGACACA AGCTCTCCAT GATGAGCAGA ATCGAACGCC ATTAATGTTT
    CAGCGTCTGG AGCCGCCATC TCCTAGCCAG GATGTGGGAC CAGGACTCAC TGGCCTTCCT
    GACACACAAA TGCACCCTGC AAATCATCTG CCTGTTGATG AAAGGATCCC AGAGAGCCAA
    TCATGGGTGA CAGCGCGAGC ACAGGAGATT TCTCAAAAAG CAGGCAACTG GAGTCCAGAG
    GGCCAACATC CAGATGACAC CACAGACAAC AGAAGGCAGT CTCAGACAGT AGAGATGAGA
    GAGATGGGGC GGGACGGGTA CTCGGACACA GAGCAATATC TGCCGATGGA AGGCCATGGC
    AGGGCTGCTT CCATGCCTCG ACTCCCAGCA GACAACCAAC AGCCTATCAC AGATAACAGT
    CCTATGAAGC GCTCGGCTTC ATCTTTGGGA CACGGGCGTG CAGGACGCTC CATGCGAGGT
    GAGGATTATG CCATGGAGAG GGTTATCCCA GAAGAGGGTC ACAGGCATGG GCATCGCCAC
    CGAGACCGTA GTCACCGGGC GTCAGAACGC TCCCTGAGCC GATACACTGA TGCTGACACA
    GGTCTTGGAA CTGACCTCAG CACAACCACA CAATCAGGAG ACCTGCCATC CAAGGAGAGA
    GACCGGGGCC GAGCCAAAGA CCGCAAGCAC CATCATCATC ATCATCACCA CCACGGCTCT
    TTAGATAAGG AGCACTACGG TCATGAGAGA GACAGAGGAG AGTACGGCCA CAGACAGTCC
    AGAGAGAGAG ACCGCCGCTG GTCCCGCTCA CCCAGTGAGG GCAGAGAGTG TCTGACTCAC
    AGACAGGGCA GTAGTTCAGT AAGCGGCAGT CCAGTCCCGT CCACCTCCGG GACCAGCACC
    CCCCGCCGTG GCCGCCGTCA GCTCCCCCAA ACCCCTGCTA CACCCCGGCC ACATGTCACC
    TACTCACCTG TGGTCCGTAA GCCCATCAGC AGTACCCCCC CTCCTGGCCA GCAGAGCCGA
    CTCCCTACCC CGACCTCCCG GCGCTTCAGT CCGACTGGCC CCGAGCCCCC GCTTCCCCCT
    CATCACCACC CGTCACCACC CCACCACGGC TCTCCCCGCT CCGGCCGACA TGCACACTGG
    GGCCCCGAGC CTGCAGAGAG CGTGGAGGGT GACGGCTTCT ATGACGACCA AGACTATGAG
    TTCAATCACC ACGAGCCTCC ATCCTACGAG CAAAGCCCTG TGCAGGGCAA CCCTCACCCC
    CATTCCCCTA GGACTTCCCG CCACAACACC CCTCCTCAGG GCCAAGCGCA CCCACGGCGC
    ATGCCAAATG GCTACCGTTC GTCTTCGCCC TCTCCACACC ACCACGCGCC CGCTCACCCT
    GGAACTCATA AACCTCCTCA TCCCAGAGGG CCCAGAAAGG GCCTCCATGA ACCCTACAGT
    GAGACTGACG AGGATGACTG GTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009297863.1
    CDS1467..8513
    Translation

    Target ORF information:

    RefSeq Version XM_009299588.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009299588.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACAGGAAGC AGCAAAGGGA AAGACATCAG TACTATCAAG
    TCTCTACGTG TGCTTCGAGT ACTGCGGCCG CTGAAAACCA TCAAACGTCT GCCAAAACTG
    AAGGCTGTCT TTGACTGTGT GGTGAACTCT CTGAAGAATG TGTTGAACAT CCTCATCGTC
    TACATGCTCT TCATGTTCAT CTTCGCTGTG GTGGCTGTGC AGCTCTTTAA GGGACGTTTC
    TTTTACTGCA CTGATGAATC CAAAGAGTTT GAGCGCGACT GCAGGGGCGA GTATCTGGTC
    TATGAAAGAG ATAACGAAGT GAGGTCACAG AAGCGGGAAT GGAAGAAATA CGATTTCCAC
    TACGACAACG TGCTCTGGGC CCTTCTCACC CTCTTCACCG TTTCTACGGG AGAGGGGTGG
    CCGCAAGTGC TAAAACATTC AGTAGATGCG ACCTACGAGA ATCAGGGCCC GAGCCCAGGT
    TACCGGATGG AAATGTCCAT CTTTTACGTG GTGTACTTCG TGGTCTTCCC CTTCTTCTTC
    GTCAACATCT TCGTGGCTCT TATCATCATT ACTTTCCAGG AGCAAGGAGA CAAAATGATG
    GAGGACTATA GCCTGGAAAA GAATGAGAGA GCCTGCATAG ACTTTGCCAT CAACGCTAAG
    CCGCTCACAC GGCACATGCC CCAGAACAAG CAGACCTTTC AGTACCGCAT GTGGGAGTTT
    GTGGTGTCTC CTCCATTTGA ATACACCATC ATGGCCTTGA TCGCGCTCAA CACTATTGTC
    CTTATGATGA AGTATGATGG AGCATCGCTT ACTTATGAAG ATGTGCTAAA ATACCTGAAC
    ATAGTGTTCA CCTCTCTATT CTCCATGGAG TGCATATTGA AGATCATAGC CTTCGGAGCA
    TTGAACTACT TCAAAGATGC CTGGAACATC TTCGACTGTG TGACTGTACT TGGCAGCATC
    ACTGATATTT TAGTCACAGA GCTGGGGAAC AACTTCATAA ATCTGAGTTT TCTAAGGCTC
    TTCAGAGCTG CTCGTCTCAT TAAGTTGCTC AGGCAGGGTG AAACCATACG CATCCTGCTC
    TGGACTTTTG TCCAGTCTTT CAAGGCTCTG CCGTATGTGT GCCTACTCAT CGCCATGCTG
    TTCTTCATCT ACGCCATCAT TGGCATGCAG CTGTTTGGGA ACATTAAGAT CGAGGAGAAC
    AGTGACAGTG CCATTACCCA GCACAACAAC TTCAGGACTT TTTTCCAGGC CCTAATGCTG
    CTCTTTAGGA GTGCTACAGG TGAGGCATGG CATGACATCA TGCTGTCATG TCTGGGAAAG
    AAGCCGTGTG ATATTCTCTC TGATAACCCG AAGCCAGAAT GCGGGAGCGA GTTTGCCTAC
    CTCTACTTTG TCTCCTTCAT CTTCCTCTGT TCATTTCTGA TGCTGAATCT GTTCGTGGCT
    GTGATCATGG ATAATTTTGA ATACCTGACG CGTGACTCCT CTATCCTGGG ACCCCATCAC
    CTGGACGAGT ATGTGAGGAT CTGGGCAGAA TATGATCCTG CTGCTTGTGG CAGAATCAGT
    TACAGGGATA TGTATGAGAT GCTCAGGCAC ATGTCTCCTC CGTTAGGCCT GGGGAAGAAG
    TGCCCTGCAC GGGTTGCCTA CAAGAGACTG TTGCGGATGG ATCTGCCGGT TGCCGATGAC
    AACACAGTGC ACTTTAATTC TACCCTTATG GCCTTAATCC GAACCGCTCT GGACATCAAG
    ATCGCCAAGG GAGGTGTAGA TAAGCACCAG ATGGATGCTG AATTAAGGAA GGAGATGATG
    GCGATATGGC CTAATCTCTC TCAGAAGAAC CTGGACCTGC TGGTGACTCC TCACAAGTCG
    ACAGACCTGA CAGTAGGAAA GATCTACGCT GCCATGATGA TCATGGAGTA TTATCGGCAA
    AGTAAAGCCA AACGGACACA AGCTCTCCAT GATGAGCAGA ATCGAACGCC ATTAATGTTT
    CAGCGTCTGG AGCCGCCATC TCCTAGCCAG GATGTGGGAC CAGGACTCAC TGGCCTTCCT
    GACACACAAA TGCACCCTGC AAATCATCTG CCTGTTGATG AAAGGATCCC AGAGAGCCAA
    TCATGGGTGA CAGCGCGAGC ACAGGAGATT TCTCAAAAAG CAGGCAACTG GAGTCCAGAG
    GGCCAACATC CAGATGACAC CACAGACAAC AGAAGGCAGT CTCAGACAGT AGAGATGAGA
    GAGATGGGGC GGGACGGGTA CTCGGACACA GAGCAATATC TGCCGATGGA AGGCCATGGC
    AGGGCTGCTT CCATGCCTCG ACTCCCAGCA GACAACCAAC AGCCTATCAC AGATAACAGT
    CCTATGAAGC GCTCGGCTTC ATCTTTGGGA CACGGGCGTG CAGGACGCTC CATGCGAGGT
    GAGGATTATG CCATGGAGAG GGTTATCCCA GAAGAGGGTC ACAGGCATGG GCATCGCCAC
    CGAGACCGTA GTCACCGGGC GTCAGAACGC TCCCTGAGCC GATACACTGA TGCTGACACA
    GGTCTTGGAA CTGACCTCAG CACAACCACA CAATCAGGAG ACCTGCCATC CAAGGAGAGA
    GACCGGGGCC GAGCCAAAGA CCGCAAGCAC CATCATCATC ATCATCACCA CCACGGCTCT
    TTAGATAAGG AGCACTACGG TCATGAGAGA GACAGAGGAG AGTACGGCCA CAGACAGTCC
    AGAGAGAGAG ACCGCCGCTG GTCCCGCTCA CCCAGTGAGG GCAGAGAGTG TCTGACTCAC
    AGACAGGGCA GTAGTTCAGT AAGCGGCAGT CCAGTCCCGT CCACCTCCGG GACCAGCACC
    CCCCGCCGTG GCCGCCGTCA GCTCCCCCAA ACCCCTGCTA CACCCCGGCC ACATGTCACC
    TACTCACCTG TGGTCCGTAA GCCCATCAGC AGTACCCCCC CTCCTGGCCA GCAGAGCCGA
    CTCCCTACCC CGACCTCCCG GCGCTTCAGT CCGACTGGCC CCGAGCCCCC GCTTCCCCCT
    CATCACCACC CGTCACCACC CCACCACGGC TCTCCCCGCT CCGGCCGACA TGCACACTGG
    GGCCCCGAGC CTGCAGAGAG CGTGGAGGGT GACGGCTTCT ATGACGACCA AGACTATGAG
    TTCAATCACC ACGAGCCTCC ATCCTACGAG CAAAGCCCTG TGCAGGGCAA CCCTCACCCC
    CATTCCCCTA GGACTTCCCG CCACAACACC CCTCCTCAGG GCCAAGCGCA CCCACGGCGC
    ATGCCAAATG GCTACCGTTC GTCTTCGCCC TCTCCACACC ACCACGCGCC CGCTCACCCT
    GGAACTCATA AACCTCCTCA TCCCAGAGGG CCCAGAAAGG GCCTCCATGA ACCCTACAGT
    GAGACTGACG AGGATGACTG GTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43664
    Clone ID Related Accession (Same CDS sequence) XM_009299589.2
    Accession Version XM_009299589.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7038bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X14
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299589.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG
    GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGAGGT GTAGATAAGC ACCAGATGGA TGCTGAATTA
    AGGAAGGAGA TGATGGCGAT ATGGCCTAAT CTCTCTCAGA AGAACCTGGA CCTGCTGGTG
    ACTCCTCACA AGTCGACAGA CCTGACAGTA GGAAAGATCT ACGCTGCCAT GATGATCATG
    GAGTATTATC GGCAAAGTAA AGCCAAACGG ACACAAGCTC TCCATGATGA GCAGCGTCTG
    GAGCCGCCAT CTCCTAGCCA GGATGTGGGA CCAGGACTCA CTGGCCTTCC TGACACACAA
    ATGCACCCTG CAAATCATCT GCCTGTTGAT GAAAGGATCC CAGAGAGCCA ATCATGGGTG
    ACAGCGCGAG CACAGGAGAT TTCTCAAAAA GCAGGCAACT GGAGTCCAGA GGGCCAACAT
    CCAGATGACA CCACAGACAA CAGAAGGCAG TCTCAGACAG TAGAGATGAG AGAGATGGGG
    CGGGACGGGT ACTCGGACAC AGAGCAATAT CTGCCGATGG AAGGCCATGG CAGGGCTGCT
    TCCATGCCTC GACTCCCAGC AGACAACCAA CAGCCTATCA CAGATAACAG TCCTATGAAG
    CGCTCGGCTT CATCTTTGGG ACACGGGCGT GCAGGACGCT CCATGCGAGG TGAGGATTAT
    GCCATGGAGA GGGTTATCCC AGAAGAGGGT CACAGGCATG GGCATCGCCA CCGAGACCGT
    AGTCACCGGG CGTCAGAACG CTCCCTGAGC CGATACACTG ATGCTGACAC AGGTCTTGGA
    ACTGACCTCA GCACAACCAC ACAATCAGGA GACCTGCCAT CCAAGGAGAG AGACCGGGGC
    CGAGCCAAAG ACCGCAAGCA CCATCATCAT CATCATCACC ACCACGGCTC TTTAGATAAG
    GAGCACTACG GTCATGAGAG AGACAGAGGA GAGTACGGCC ACAGACAGTC CAGAGAGAGA
    GACCGCCGCT GGTCCCGCTC ACCCAGTGAG GGCAGAGAGT GTCTGACTCA CAGACAGGGC
    AGTAGTTCAG TAAGCGGCAG TCCAGTCCCG TCCACCTCCG GGACCAGCAC CCCCCGCCGT
    GGCCGCCGTC AGCTCCCCCA AACCCCTGCT ACACCCCGGC CACATGTCAC CTACTCACCT
    GTGGTCCGTA AGCCCATCAG CAGTACCCCC CCTCCTGGCC AGCAGAGCCG ACTCCCTACC
    CCGACCTCCC GGCGCTTCAG TCCGACTGGC CCCGAGCCCC CGCTTCCCCC TCATCACCAC
    CCGTCACCAC CCCACCACGG CTCTCCCCGC TCCGGCCGAC ATGCACACTG GGGCCCCGAG
    CCTGCAGAGA GCGTGGAGGG TGACGGCTTC TATGACGACC AAGACTATGA GTTCAATCAC
    CACGAGCCTC CATCCTACGA GCAAAGCCCT GTGCAGGGCA ACCCTCACCC CCATTCCCCT
    AGGACTTCCC GCCACAACAC CCCTCCTCAG GGCCAAGCGC ACCCACGGCG CATGCCAAAT
    GGCTACCGTT CGTCTTCGCC CTCTCCACAC CACCACGCGC CCGCTCACCC TGGAACTCAT
    AAACCTCCTC ATCCCAGAGG GCCCAGAAAG GGCCTCCATG AACCCTACAG TGAGACTGAC
    GAGGATGACT GGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009297864.1
    CDS1469..8506
    Translation

    Target ORF information:

    RefSeq Version XM_009299589.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009299589.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG
    GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGAGGT GTAGATAAGC ACCAGATGGA TGCTGAATTA
    AGGAAGGAGA TGATGGCGAT ATGGCCTAAT CTCTCTCAGA AGAACCTGGA CCTGCTGGTG
    ACTCCTCACA AGTCGACAGA CCTGACAGTA GGAAAGATCT ACGCTGCCAT GATGATCATG
    GAGTATTATC GGCAAAGTAA AGCCAAACGG ACACAAGCTC TCCATGATGA GCAGCGTCTG
    GAGCCGCCAT CTCCTAGCCA GGATGTGGGA CCAGGACTCA CTGGCCTTCC TGACACACAA
    ATGCACCCTG CAAATCATCT GCCTGTTGAT GAAAGGATCC CAGAGAGCCA ATCATGGGTG
    ACAGCGCGAG CACAGGAGAT TTCTCAAAAA GCAGGCAACT GGAGTCCAGA GGGCCAACAT
    CCAGATGACA CCACAGACAA CAGAAGGCAG TCTCAGACAG TAGAGATGAG AGAGATGGGG
    CGGGACGGGT ACTCGGACAC AGAGCAATAT CTGCCGATGG AAGGCCATGG CAGGGCTGCT
    TCCATGCCTC GACTCCCAGC AGACAACCAA CAGCCTATCA CAGATAACAG TCCTATGAAG
    CGCTCGGCTT CATCTTTGGG ACACGGGCGT GCAGGACGCT CCATGCGAGG TGAGGATTAT
    GCCATGGAGA GGGTTATCCC AGAAGAGGGT CACAGGCATG GGCATCGCCA CCGAGACCGT
    AGTCACCGGG CGTCAGAACG CTCCCTGAGC CGATACACTG ATGCTGACAC AGGTCTTGGA
    ACTGACCTCA GCACAACCAC ACAATCAGGA GACCTGCCAT CCAAGGAGAG AGACCGGGGC
    CGAGCCAAAG ACCGCAAGCA CCATCATCAT CATCATCACC ACCACGGCTC TTTAGATAAG
    GAGCACTACG GTCATGAGAG AGACAGAGGA GAGTACGGCC ACAGACAGTC CAGAGAGAGA
    GACCGCCGCT GGTCCCGCTC ACCCAGTGAG GGCAGAGAGT GTCTGACTCA CAGACAGGGC
    AGTAGTTCAG TAAGCGGCAG TCCAGTCCCG TCCACCTCCG GGACCAGCAC CCCCCGCCGT
    GGCCGCCGTC AGCTCCCCCA AACCCCTGCT ACACCCCGGC CACATGTCAC CTACTCACCT
    GTGGTCCGTA AGCCCATCAG CAGTACCCCC CCTCCTGGCC AGCAGAGCCG ACTCCCTACC
    CCGACCTCCC GGCGCTTCAG TCCGACTGGC CCCGAGCCCC CGCTTCCCCC TCATCACCAC
    CCGTCACCAC CCCACCACGG CTCTCCCCGC TCCGGCCGAC ATGCACACTG GGGCCCCGAG
    CCTGCAGAGA GCGTGGAGGG TGACGGCTTC TATGACGACC AAGACTATGA GTTCAATCAC
    CACGAGCCTC CATCCTACGA GCAAAGCCCT GTGCAGGGCA ACCCTCACCC CCATTCCCCT
    AGGACTTCCC GCCACAACAC CCCTCCTCAG GGCCAAGCGC ACCCACGGCG CATGCCAAAT
    GGCTACCGTT CGTCTTCGCC CTCTCCACAC CACCACGCGC CCGCTCACCC TGGAACTCAT
    AAACCTCCTC ATCCCAGAGG GCCCAGAAAG GGCCTCCATG AACCCTACAG TGAGACTGAC
    GAGGATGACT GGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43665
    Clone ID Related Accession (Same CDS sequence) XM_009299590.3 , XM_009299590.2
    Accession Version XM_009299590.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6957bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009299590.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG
    GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT
    GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC
    CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC
    ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT
    GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT
    GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT
    GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT
    CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA
    AGGCAGTCTC AGCCTATCAC AGATAACAGT CCTATGAAGC GCTCGGCTTC ATCTTTGGGA
    CACGGGCGTG CAGGACGCTC CATGCGAGGT GAGGATTATG CCATGGAGAG GGTTATCCCA
    GAAGAGGGTC ACAGGCATGG GCATCGCCAC CGAGACCGTA GTCACCGGGC GTCAGAACGC
    TCCCTGAGCC GATACACTGA TGCTGACACA GGTCTTGGAA CTGACCTCAG CACAACCACA
    CAATCAGGAG ACCTGCCATC CAAGGAGAGA GACCGGGGCC GAGCCAAAGA CCGCAAGCAC
    CATCATCATC ATCATCACCA CCACGGCTCT TTAGATAAGG AGCACTACGG TCATGAGAGA
    GACAGAGGAG AGTACGGCCA CAGACAGTCC AGAGAGAGAG ACCGCCGCTG GTCCCGCTCA
    CCCAGTGAGG GCAGAGAGTG TCTGACTCAC AGACAGGGCA GTAGTTCAGT AAGCGGCAGT
    CCAGTCCCGT CCACCTCCGG GACCAGCACC CCCCGCCGTG GCCGCCGTCA GCTCCCCCAA
    ACCCCTGCTA CACCCCGGCC ACATGTCACC TACTCACCTG TGGTCCGTAA GCCCATCAGC
    AGTACCCCCC CTCCTGGCCA GCAGAGCCGA CTCCCTACCC CGACCTCCCG GCGCTTCAGT
    CCGACTGGCC CCGAGCCCCC GCTTCCCCCT CATCACCACC CGTCACCACC CCACCACGGC
    TCTCCCCGCT CCGGCCGACA TGCACACTGG GGCCCCGAGC CTGCAGAGAG CGTGGAGGGT
    GACGGCTTCT ATGACGACCA AGACTATGAG TTCAATCACC ACGAGCCTCC ATCCTACGAG
    CAAAGCCCTG TGCAGGGCAA CCCTCACCCC CATTCCCCTA GGACTTCCCG CCACAACACC
    CCTCCTCAGG GCCAAGCGCA CCCACGGCGC ATGCCAAATG GCTACCGTTC GTCTTCGCCC
    TCTCCACACC ACCACGCGCC CGCTCACCCT GGAACTCATA AACCTCCTCA TCCCAGAGGG
    CCCAGAAAGG GCCTCCATGA ACCCTACAGT GAGACTGACG AGGATGACTG GTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009297865.1
    CDS1508..8464
    Translation

    Target ORF information:

    RefSeq Version XM_009299590.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009299590.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG
    GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT
    GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC
    CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC
    ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT
    GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT
    GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT
    GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT
    CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA
    AGGCAGTCTC AGCCTATCAC AGATAACAGT CCTATGAAGC GCTCGGCTTC ATCTTTGGGA
    CACGGGCGTG CAGGACGCTC CATGCGAGGT GAGGATTATG CCATGGAGAG GGTTATCCCA
    GAAGAGGGTC ACAGGCATGG GCATCGCCAC CGAGACCGTA GTCACCGGGC GTCAGAACGC
    TCCCTGAGCC GATACACTGA TGCTGACACA GGTCTTGGAA CTGACCTCAG CACAACCACA
    CAATCAGGAG ACCTGCCATC CAAGGAGAGA GACCGGGGCC GAGCCAAAGA CCGCAAGCAC
    CATCATCATC ATCATCACCA CCACGGCTCT TTAGATAAGG AGCACTACGG TCATGAGAGA
    GACAGAGGAG AGTACGGCCA CAGACAGTCC AGAGAGAGAG ACCGCCGCTG GTCCCGCTCA
    CCCAGTGAGG GCAGAGAGTG TCTGACTCAC AGACAGGGCA GTAGTTCAGT AAGCGGCAGT
    CCAGTCCCGT CCACCTCCGG GACCAGCACC CCCCGCCGTG GCCGCCGTCA GCTCCCCCAA
    ACCCCTGCTA CACCCCGGCC ACATGTCACC TACTCACCTG TGGTCCGTAA GCCCATCAGC
    AGTACCCCCC CTCCTGGCCA GCAGAGCCGA CTCCCTACCC CGACCTCCCG GCGCTTCAGT
    CCGACTGGCC CCGAGCCCCC GCTTCCCCCT CATCACCACC CGTCACCACC CCACCACGGC
    TCTCCCCGCT CCGGCCGACA TGCACACTGG GGCCCCGAGC CTGCAGAGAG CGTGGAGGGT
    GACGGCTTCT ATGACGACCA AGACTATGAG TTCAATCACC ACGAGCCTCC ATCCTACGAG
    CAAAGCCCTG TGCAGGGCAA CCCTCACCCC CATTCCCCTA GGACTTCCCG CCACAACACC
    CCTCCTCAGG GCCAAGCGCA CCCACGGCGC ATGCCAAATG GCTACCGTTC GTCTTCGCCC
    TCTCCACACC ACCACGCGCC CGCTCACCCT GGAACTCATA AACCTCCTCA TCCCAGAGGG
    CCCAGAAAGG GCCTCCATGA ACCCTACAGT GAGACTGACG AGGATGACTG GTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43665
    Clone ID Related Accession (Same CDS sequence) XM_009299590.3 , XM_009299590.2
    Accession Version XM_009299590.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6957bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X16
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299590.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG
    GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT
    GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC
    CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC
    ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT
    GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT
    GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT
    GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT
    CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA
    AGGCAGTCTC AGCCTATCAC AGATAACAGT CCTATGAAGC GCTCGGCTTC ATCTTTGGGA
    CACGGGCGTG CAGGACGCTC CATGCGAGGT GAGGATTATG CCATGGAGAG GGTTATCCCA
    GAAGAGGGTC ACAGGCATGG GCATCGCCAC CGAGACCGTA GTCACCGGGC GTCAGAACGC
    TCCCTGAGCC GATACACTGA TGCTGACACA GGTCTTGGAA CTGACCTCAG CACAACCACA
    CAATCAGGAG ACCTGCCATC CAAGGAGAGA GACCGGGGCC GAGCCAAAGA CCGCAAGCAC
    CATCATCATC ATCATCACCA CCACGGCTCT TTAGATAAGG AGCACTACGG TCATGAGAGA
    GACAGAGGAG AGTACGGCCA CAGACAGTCC AGAGAGAGAG ACCGCCGCTG GTCCCGCTCA
    CCCAGTGAGG GCAGAGAGTG TCTGACTCAC AGACAGGGCA GTAGTTCAGT AAGCGGCAGT
    CCAGTCCCGT CCACCTCCGG GACCAGCACC CCCCGCCGTG GCCGCCGTCA GCTCCCCCAA
    ACCCCTGCTA CACCCCGGCC ACATGTCACC TACTCACCTG TGGTCCGTAA GCCCATCAGC
    AGTACCCCCC CTCCTGGCCA GCAGAGCCGA CTCCCTACCC CGACCTCCCG GCGCTTCAGT
    CCGACTGGCC CCGAGCCCCC GCTTCCCCCT CATCACCACC CGTCACCACC CCACCACGGC
    TCTCCCCGCT CCGGCCGACA TGCACACTGG GGCCCCGAGC CTGCAGAGAG CGTGGAGGGT
    GACGGCTTCT ATGACGACCA AGACTATGAG TTCAATCACC ACGAGCCTCC ATCCTACGAG
    CAAAGCCCTG TGCAGGGCAA CCCTCACCCC CATTCCCCTA GGACTTCCCG CCACAACACC
    CCTCCTCAGG GCCAAGCGCA CCCACGGCGC ATGCCAAATG GCTACCGTTC GTCTTCGCCC
    TCTCCACACC ACCACGCGCC CGCTCACCCT GGAACTCATA AACCTCCTCA TCCCAGAGGG
    CCCAGAAAGG GCCTCCATGA ACCCTACAGT GAGACTGACG AGGATGACTG GTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009297865.1
    CDS1468..8424
    Translation

    Target ORF information:

    RefSeq Version XM_009299590.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009299590.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG
    GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT
    GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC
    CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC
    ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT
    GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT
    GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT
    GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT
    CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA
    AGGCAGTCTC AGCCTATCAC AGATAACAGT CCTATGAAGC GCTCGGCTTC ATCTTTGGGA
    CACGGGCGTG CAGGACGCTC CATGCGAGGT GAGGATTATG CCATGGAGAG GGTTATCCCA
    GAAGAGGGTC ACAGGCATGG GCATCGCCAC CGAGACCGTA GTCACCGGGC GTCAGAACGC
    TCCCTGAGCC GATACACTGA TGCTGACACA GGTCTTGGAA CTGACCTCAG CACAACCACA
    CAATCAGGAG ACCTGCCATC CAAGGAGAGA GACCGGGGCC GAGCCAAAGA CCGCAAGCAC
    CATCATCATC ATCATCACCA CCACGGCTCT TTAGATAAGG AGCACTACGG TCATGAGAGA
    GACAGAGGAG AGTACGGCCA CAGACAGTCC AGAGAGAGAG ACCGCCGCTG GTCCCGCTCA
    CCCAGTGAGG GCAGAGAGTG TCTGACTCAC AGACAGGGCA GTAGTTCAGT AAGCGGCAGT
    CCAGTCCCGT CCACCTCCGG GACCAGCACC CCCCGCCGTG GCCGCCGTCA GCTCCCCCAA
    ACCCCTGCTA CACCCCGGCC ACATGTCACC TACTCACCTG TGGTCCGTAA GCCCATCAGC
    AGTACCCCCC CTCCTGGCCA GCAGAGCCGA CTCCCTACCC CGACCTCCCG GCGCTTCAGT
    CCGACTGGCC CCGAGCCCCC GCTTCCCCCT CATCACCACC CGTCACCACC CCACCACGGC
    TCTCCCCGCT CCGGCCGACA TGCACACTGG GGCCCCGAGC CTGCAGAGAG CGTGGAGGGT
    GACGGCTTCT ATGACGACCA AGACTATGAG TTCAATCACC ACGAGCCTCC ATCCTACGAG
    CAAAGCCCTG TGCAGGGCAA CCCTCACCCC CATTCCCCTA GGACTTCCCG CCACAACACC
    CCTCCTCAGG GCCAAGCGCA CCCACGGCGC ATGCCAAATG GCTACCGTTC GTCTTCGCCC
    TCTCCACACC ACCACGCGCC CGCTCACCCT GGAACTCATA AACCTCCTCA TCCCAGAGGG
    CCCAGAAAGG GCCTCCATGA ACCCTACAGT GAGACTGACG AGGATGACTG GTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43666
    Clone ID Related Accession (Same CDS sequence) XM_009299591.2 , XM_009299591.3
    Accession Version XM_009299591.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6933bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X17
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299591.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACAGGAAGC AGCAAAGGGA AAGACATCAG TACTATCAAG
    TCTCTACGTG TGCTTCGAGT ACTGCGGCCG CTGAAAACCA TCAAACGTCT GCCAAAACTG
    AAGGCTGTCT TTGACTGTGT GGTGAACTCT CTGAAGAATG TGTTGAACAT CCTCATCGTC
    TACATGCTCT TCATGTTCAT CTTCGCTGTG GTGGCTGTGC AGCTCTTTAA GGGACGTTTC
    TTTTACTGCA CTGATGAATC CAAAGAGTTT GAGCGCGACT GCAGGGGCGA GTATCTGGTC
    TATGAAAGAG ATAACGAAGT GAGGTCACAG AAGCGGGAAT GGAAGAAATA CGATTTCCAC
    TACGACAACG TGCTCTGGGC CCTTCTCACC CTCTTCACCG TTTCTACGGG AGAGGGGTGG
    CCGCAAGTGC TAAAACATTC AGTAGATGCG ACCTACGAGA ATCAGGGCCC GAGCCCAGGT
    TACCGGATGG AAATGTCCAT CTTTTACGTG GTGTACTTCG TGGTCTTCCC CTTCTTCTTC
    GTCAACATCT TCGTGGCTCT TATCATCATT ACTTTCCAGG AGCAAGGAGA CAAAATGATG
    GAGGACTATA GCCTGGAAAA GAATGAGAGA GCCTGCATAG ACTTTGCCAT CAACGCTAAG
    CCGCTCACAC GGCACATGCC CCAGAACAAG CAGACCTTTC AGTACCGCAT GTGGGAGTTT
    GTGGTGTCTC CTCCATTTGA ATACACCATC ATGGCCTTGA TCGCGCTCAA CACTATTGTC
    CTTATGATGA AGTATGATGG AGCATCGCTT ACTTATGAAG ATGTGCTAAA ATACCTGAAC
    ATAGTGTTCA CCTCTCTATT CTCCATGGAG TGCATATTGA AGATCATAGC CTTCGGAGCA
    TTGAACTACT TCAAAGATGC CTGGAACATC TTCGACTGTG TGACTGTACT TGGCAGCATC
    ACTGATATTT TAGTCACAGA GCTGGGGAAC AACTTCATAA ATCTGAGTTT TCTAAGGCTC
    TTCAGAGCTG CTCGTCTCAT TAAGTTGCTC AGGCAGGGTG AAACCATACG CATCCTGCTC
    TGGACTTTTG TCCAGTCTTT CAAGGCTCTG CCGTATGTGT GCCTACTCAT CGCCATGCTG
    TTCTTCATCT ACGCCATCAT TGGCATGCAG CTGTTTGGGA ACATTAAGAT CGAGGAGAAC
    AGTGACAGTG CCATTACCCA GCACAACAAC TTCAGGACTT TTTTCCAGGC CCTAATGCTG
    CTCTTTAGGA GTGCTACAGG TGAGGCATGG CATGACATCA TGCTGTCATG TCTGGGAAAG
    AAGCCGTGTG ATATTCTCTC TGATAACCCG AAGCCAGAAT GCGGGAGCGA GTTTGCCTAC
    CTCTACTTTG TCTCCTTCAT CTTCCTCTGT TCATTTCTGA TGCTGAATCT GTTCGTGGCT
    GTGATCATGG ATAATTTTGA ATACCTGACG CGTGACTCCT CTATCCTGGG ACCCCATCAC
    CTGGACGAGT ATGTGAGGAT CTGGGCAGAA TATGATCCTG CTGCTTGCGG GCGCATTCAT
    TATAAGGATA TGTACAGTTT ATTGCGAGTT ATCGACCCCC CTCTGGGCTT AGGCAAGAAA
    TGTCCTCATA GGGTGGCCTG CAAGAGACTG TTGCGGATGG ATCTGCCGGT TGCCGATGAC
    AACACAGTGC ACTTTAATTC TACCCTTATG GCCTTAATCC GAACCGCTCT GGACATCAAG
    ATCGCCAAGG GAGGTGTAGA TAAGCACCAG ATGGATGCTG AATTAAGGAA GGAGATGATG
    GCGATATGGC CTAATCTCTC TCAGAAGAAC CTGGACCTGC TGGTGACTCC TCACAAGTCA
    GCGACAGACC TGACAGTAGG AAAGATCTAC GCTGCCATGA TGATCATGGA GTATTATCGG
    CAAAGTAAAG CCAAACGGAC ACAAGCTCTC CATGATGAGC AGAATCGAAC GCCATTAATG
    TTTCAGCGTC TGGAGCCGCC ATCTCCTAGC CAGGATGTGG GACCAGGACT CACTGGCCTT
    CCTGACACAC AAATGCACCC TGCAAATCAT CTGCCTGTTG ATGAAAGGAT CCCAGAGAGC
    CAATCATGGG TGACAGCGCG AGCACAGGAG ATTTCTCAAA AAGCAGGCAA CTGGAGTCCA
    GAGGGCCAAC ATCCAGATGA CACCACAGAC AACAGAAGGC AGTCTCAGCC TATCACAGAT
    AACAGTCCTA TGAAGCGCTC GGCTTCATCT TTGGGACACG GGCGTGCAGG ACGCTCCATG
    CGAGGTGAGG ATTATGCCAT GGAGAGGGTT ATCCCAGAAG AGGGTCACAG GCATGGGCAT
    CGCCACCGAG ACCGTAGTCA CCGGGCGTCA GAACGCTCCC TGAGCCGATA CACTGATGCT
    GACACAGGTC TTGGAACTGA CCTCAGCACA ACCACACAAT CAGGAGACCT GCCATCCAAG
    GAGAGAGACC GGGGCCGAGC CAAAGACCGC AAGCACCATC ATCATCATCA TCACCACCAC
    GGCTCTTTAG ATAAGGAGCA CTACGGTCAT GAGAGAGACA GAGGAGAGTA CGGCCACAGA
    CAGTCCAGAG AGAGAGACCG CCGCTGGTCC CGCTCACCCA GTGAGGGCAG AGAGTGTCTG
    ACTCACAGAC AGGGCAGTAG TTCAGTAAGC GGCAGTCCAG TCCCGTCCAC CTCCGGGACC
    AGCACCCCCC GCCGTGGCCG CCGTCAGCTC CCCCAAACCC CTGCTACACC CCGGCCACAT
    GTCACCTACT CACCTGTGGT CCGTAAGCCC ATCAGCAGTA CCCCCCCTCC TGGCCAGCAG
    AGCCGACTCC CTACCCCGAC CTCCCGGCGC TTCAGTCCGA CTGGCCCCGA GCCCCCGCTT
    CCCCCTCATC ACCACCCGTC ACCACCCCAC CACGGCTCTC CCCGCTCCGG CCGACATGCA
    CACTGGGGCC CCGAGCCTGC AGAGAGCGTG GAGGGTGACG GCTTCTATGA CGACCAAGAC
    TATGAGTTCA ATCACCACGA GCCTCCATCC TACGAGCAAA GCCCTGTGCA GGGCAACCCT
    CACCCCCATT CCCCTAGGAC TTCCCGCCAC AACACCCCTC CTCAGGGCCA AGCGCACCCA
    CGGCGCATGC CAAATGGCTA CCGTTCGTCT TCGCCCTCTC CACACCACCA CGCGCCCGCT
    CACCCTGGAA CTCATAAACC TCCTCATCCC AGAGGGCCCA GAAAGGGCCT CCATGAACCC
    TACAGTGAGA CTGACGAGGA TGACTGGTGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009297866.1
    CDS1467..8399
    Translation

    Target ORF information:

    RefSeq Version XM_009299591.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009299591.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACAGGAAGC AGCAAAGGGA AAGACATCAG TACTATCAAG
    TCTCTACGTG TGCTTCGAGT ACTGCGGCCG CTGAAAACCA TCAAACGTCT GCCAAAACTG
    AAGGCTGTCT TTGACTGTGT GGTGAACTCT CTGAAGAATG TGTTGAACAT CCTCATCGTC
    TACATGCTCT TCATGTTCAT CTTCGCTGTG GTGGCTGTGC AGCTCTTTAA GGGACGTTTC
    TTTTACTGCA CTGATGAATC CAAAGAGTTT GAGCGCGACT GCAGGGGCGA GTATCTGGTC
    TATGAAAGAG ATAACGAAGT GAGGTCACAG AAGCGGGAAT GGAAGAAATA CGATTTCCAC
    TACGACAACG TGCTCTGGGC CCTTCTCACC CTCTTCACCG TTTCTACGGG AGAGGGGTGG
    CCGCAAGTGC TAAAACATTC AGTAGATGCG ACCTACGAGA ATCAGGGCCC GAGCCCAGGT
    TACCGGATGG AAATGTCCAT CTTTTACGTG GTGTACTTCG TGGTCTTCCC CTTCTTCTTC
    GTCAACATCT TCGTGGCTCT TATCATCATT ACTTTCCAGG AGCAAGGAGA CAAAATGATG
    GAGGACTATA GCCTGGAAAA GAATGAGAGA GCCTGCATAG ACTTTGCCAT CAACGCTAAG
    CCGCTCACAC GGCACATGCC CCAGAACAAG CAGACCTTTC AGTACCGCAT GTGGGAGTTT
    GTGGTGTCTC CTCCATTTGA ATACACCATC ATGGCCTTGA TCGCGCTCAA CACTATTGTC
    CTTATGATGA AGTATGATGG AGCATCGCTT ACTTATGAAG ATGTGCTAAA ATACCTGAAC
    ATAGTGTTCA CCTCTCTATT CTCCATGGAG TGCATATTGA AGATCATAGC CTTCGGAGCA
    TTGAACTACT TCAAAGATGC CTGGAACATC TTCGACTGTG TGACTGTACT TGGCAGCATC
    ACTGATATTT TAGTCACAGA GCTGGGGAAC AACTTCATAA ATCTGAGTTT TCTAAGGCTC
    TTCAGAGCTG CTCGTCTCAT TAAGTTGCTC AGGCAGGGTG AAACCATACG CATCCTGCTC
    TGGACTTTTG TCCAGTCTTT CAAGGCTCTG CCGTATGTGT GCCTACTCAT CGCCATGCTG
    TTCTTCATCT ACGCCATCAT TGGCATGCAG CTGTTTGGGA ACATTAAGAT CGAGGAGAAC
    AGTGACAGTG CCATTACCCA GCACAACAAC TTCAGGACTT TTTTCCAGGC CCTAATGCTG
    CTCTTTAGGA GTGCTACAGG TGAGGCATGG CATGACATCA TGCTGTCATG TCTGGGAAAG
    AAGCCGTGTG ATATTCTCTC TGATAACCCG AAGCCAGAAT GCGGGAGCGA GTTTGCCTAC
    CTCTACTTTG TCTCCTTCAT CTTCCTCTGT TCATTTCTGA TGCTGAATCT GTTCGTGGCT
    GTGATCATGG ATAATTTTGA ATACCTGACG CGTGACTCCT CTATCCTGGG ACCCCATCAC
    CTGGACGAGT ATGTGAGGAT CTGGGCAGAA TATGATCCTG CTGCTTGCGG GCGCATTCAT
    TATAAGGATA TGTACAGTTT ATTGCGAGTT ATCGACCCCC CTCTGGGCTT AGGCAAGAAA
    TGTCCTCATA GGGTGGCCTG CAAGAGACTG TTGCGGATGG ATCTGCCGGT TGCCGATGAC
    AACACAGTGC ACTTTAATTC TACCCTTATG GCCTTAATCC GAACCGCTCT GGACATCAAG
    ATCGCCAAGG GAGGTGTAGA TAAGCACCAG ATGGATGCTG AATTAAGGAA GGAGATGATG
    GCGATATGGC CTAATCTCTC TCAGAAGAAC CTGGACCTGC TGGTGACTCC TCACAAGTCA
    GCGACAGACC TGACAGTAGG AAAGATCTAC GCTGCCATGA TGATCATGGA GTATTATCGG
    CAAAGTAAAG CCAAACGGAC ACAAGCTCTC CATGATGAGC AGAATCGAAC GCCATTAATG
    TTTCAGCGTC TGGAGCCGCC ATCTCCTAGC CAGGATGTGG GACCAGGACT CACTGGCCTT
    CCTGACACAC AAATGCACCC TGCAAATCAT CTGCCTGTTG ATGAAAGGAT CCCAGAGAGC
    CAATCATGGG TGACAGCGCG AGCACAGGAG ATTTCTCAAA AAGCAGGCAA CTGGAGTCCA
    GAGGGCCAAC ATCCAGATGA CACCACAGAC AACAGAAGGC AGTCTCAGCC TATCACAGAT
    AACAGTCCTA TGAAGCGCTC GGCTTCATCT TTGGGACACG GGCGTGCAGG ACGCTCCATG
    CGAGGTGAGG ATTATGCCAT GGAGAGGGTT ATCCCAGAAG AGGGTCACAG GCATGGGCAT
    CGCCACCGAG ACCGTAGTCA CCGGGCGTCA GAACGCTCCC TGAGCCGATA CACTGATGCT
    GACACAGGTC TTGGAACTGA CCTCAGCACA ACCACACAAT CAGGAGACCT GCCATCCAAG
    GAGAGAGACC GGGGCCGAGC CAAAGACCGC AAGCACCATC ATCATCATCA TCACCACCAC
    GGCTCTTTAG ATAAGGAGCA CTACGGTCAT GAGAGAGACA GAGGAGAGTA CGGCCACAGA
    CAGTCCAGAG AGAGAGACCG CCGCTGGTCC CGCTCACCCA GTGAGGGCAG AGAGTGTCTG
    ACTCACAGAC AGGGCAGTAG TTCAGTAAGC GGCAGTCCAG TCCCGTCCAC CTCCGGGACC
    AGCACCCCCC GCCGTGGCCG CCGTCAGCTC CCCCAAACCC CTGCTACACC CCGGCCACAT
    GTCACCTACT CACCTGTGGT CCGTAAGCCC ATCAGCAGTA CCCCCCCTCC TGGCCAGCAG
    AGCCGACTCC CTACCCCGAC CTCCCGGCGC TTCAGTCCGA CTGGCCCCGA GCCCCCGCTT
    CCCCCTCATC ACCACCCGTC ACCACCCCAC CACGGCTCTC CCCGCTCCGG CCGACATGCA
    CACTGGGGCC CCGAGCCTGC AGAGAGCGTG GAGGGTGACG GCTTCTATGA CGACCAAGAC
    TATGAGTTCA ATCACCACGA GCCTCCATCC TACGAGCAAA GCCCTGTGCA GGGCAACCCT
    CACCCCCATT CCCCTAGGAC TTCCCGCCAC AACACCCCTC CTCAGGGCCA AGCGCACCCA
    CGGCGCATGC CAAATGGCTA CCGTTCGTCT TCGCCCTCTC CACACCACCA CGCGCCCGCT
    CACCCTGGAA CTCATAAACC TCCTCATCCC AGAGGGCCCA GAAAGGGCCT CCATGAACCC
    TACAGTGAGA CTGACGAGGA TGACTGGTGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43666
    Clone ID Related Accession (Same CDS sequence) XM_009299591.2 , XM_009299591.3
    Accession Version XM_009299591.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6933bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009299591.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACAGGAAGC AGCAAAGGGA AAGACATCAG TACTATCAAG
    TCTCTACGTG TGCTTCGAGT ACTGCGGCCG CTGAAAACCA TCAAACGTCT GCCAAAACTG
    AAGGCTGTCT TTGACTGTGT GGTGAACTCT CTGAAGAATG TGTTGAACAT CCTCATCGTC
    TACATGCTCT TCATGTTCAT CTTCGCTGTG GTGGCTGTGC AGCTCTTTAA GGGACGTTTC
    TTTTACTGCA CTGATGAATC CAAAGAGTTT GAGCGCGACT GCAGGGGCGA GTATCTGGTC
    TATGAAAGAG ATAACGAAGT GAGGTCACAG AAGCGGGAAT GGAAGAAATA CGATTTCCAC
    TACGACAACG TGCTCTGGGC CCTTCTCACC CTCTTCACCG TTTCTACGGG AGAGGGGTGG
    CCGCAAGTGC TAAAACATTC AGTAGATGCG ACCTACGAGA ATCAGGGCCC GAGCCCAGGT
    TACCGGATGG AAATGTCCAT CTTTTACGTG GTGTACTTCG TGGTCTTCCC CTTCTTCTTC
    GTCAACATCT TCGTGGCTCT TATCATCATT ACTTTCCAGG AGCAAGGAGA CAAAATGATG
    GAGGACTATA GCCTGGAAAA GAATGAGAGA GCCTGCATAG ACTTTGCCAT CAACGCTAAG
    CCGCTCACAC GGCACATGCC CCAGAACAAG CAGACCTTTC AGTACCGCAT GTGGGAGTTT
    GTGGTGTCTC CTCCATTTGA ATACACCATC ATGGCCTTGA TCGCGCTCAA CACTATTGTC
    CTTATGATGA AGTATGATGG AGCATCGCTT ACTTATGAAG ATGTGCTAAA ATACCTGAAC
    ATAGTGTTCA CCTCTCTATT CTCCATGGAG TGCATATTGA AGATCATAGC CTTCGGAGCA
    TTGAACTACT TCAAAGATGC CTGGAACATC TTCGACTGTG TGACTGTACT TGGCAGCATC
    ACTGATATTT TAGTCACAGA GCTGGGGAAC AACTTCATAA ATCTGAGTTT TCTAAGGCTC
    TTCAGAGCTG CTCGTCTCAT TAAGTTGCTC AGGCAGGGTG AAACCATACG CATCCTGCTC
    TGGACTTTTG TCCAGTCTTT CAAGGCTCTG CCGTATGTGT GCCTACTCAT CGCCATGCTG
    TTCTTCATCT ACGCCATCAT TGGCATGCAG CTGTTTGGGA ACATTAAGAT CGAGGAGAAC
    AGTGACAGTG CCATTACCCA GCACAACAAC TTCAGGACTT TTTTCCAGGC CCTAATGCTG
    CTCTTTAGGA GTGCTACAGG TGAGGCATGG CATGACATCA TGCTGTCATG TCTGGGAAAG
    AAGCCGTGTG ATATTCTCTC TGATAACCCG AAGCCAGAAT GCGGGAGCGA GTTTGCCTAC
    CTCTACTTTG TCTCCTTCAT CTTCCTCTGT TCATTTCTGA TGCTGAATCT GTTCGTGGCT
    GTGATCATGG ATAATTTTGA ATACCTGACG CGTGACTCCT CTATCCTGGG ACCCCATCAC
    CTGGACGAGT ATGTGAGGAT CTGGGCAGAA TATGATCCTG CTGCTTGCGG GCGCATTCAT
    TATAAGGATA TGTACAGTTT ATTGCGAGTT ATCGACCCCC CTCTGGGCTT AGGCAAGAAA
    TGTCCTCATA GGGTGGCCTG CAAGAGACTG TTGCGGATGG ATCTGCCGGT TGCCGATGAC
    AACACAGTGC ACTTTAATTC TACCCTTATG GCCTTAATCC GAACCGCTCT GGACATCAAG
    ATCGCCAAGG GAGGTGTAGA TAAGCACCAG ATGGATGCTG AATTAAGGAA GGAGATGATG
    GCGATATGGC CTAATCTCTC TCAGAAGAAC CTGGACCTGC TGGTGACTCC TCACAAGTCA
    GCGACAGACC TGACAGTAGG AAAGATCTAC GCTGCCATGA TGATCATGGA GTATTATCGG
    CAAAGTAAAG CCAAACGGAC ACAAGCTCTC CATGATGAGC AGAATCGAAC GCCATTAATG
    TTTCAGCGTC TGGAGCCGCC ATCTCCTAGC CAGGATGTGG GACCAGGACT CACTGGCCTT
    CCTGACACAC AAATGCACCC TGCAAATCAT CTGCCTGTTG ATGAAAGGAT CCCAGAGAGC
    CAATCATGGG TGACAGCGCG AGCACAGGAG ATTTCTCAAA AAGCAGGCAA CTGGAGTCCA
    GAGGGCCAAC ATCCAGATGA CACCACAGAC AACAGAAGGC AGTCTCAGCC TATCACAGAT
    AACAGTCCTA TGAAGCGCTC GGCTTCATCT TTGGGACACG GGCGTGCAGG ACGCTCCATG
    CGAGGTGAGG ATTATGCCAT GGAGAGGGTT ATCCCAGAAG AGGGTCACAG GCATGGGCAT
    CGCCACCGAG ACCGTAGTCA CCGGGCGTCA GAACGCTCCC TGAGCCGATA CACTGATGCT
    GACACAGGTC TTGGAACTGA CCTCAGCACA ACCACACAAT CAGGAGACCT GCCATCCAAG
    GAGAGAGACC GGGGCCGAGC CAAAGACCGC AAGCACCATC ATCATCATCA TCACCACCAC
    GGCTCTTTAG ATAAGGAGCA CTACGGTCAT GAGAGAGACA GAGGAGAGTA CGGCCACAGA
    CAGTCCAGAG AGAGAGACCG CCGCTGGTCC CGCTCACCCA GTGAGGGCAG AGAGTGTCTG
    ACTCACAGAC AGGGCAGTAG TTCAGTAAGC GGCAGTCCAG TCCCGTCCAC CTCCGGGACC
    AGCACCCCCC GCCGTGGCCG CCGTCAGCTC CCCCAAACCC CTGCTACACC CCGGCCACAT
    GTCACCTACT CACCTGTGGT CCGTAAGCCC ATCAGCAGTA CCCCCCCTCC TGGCCAGCAG
    AGCCGACTCC CTACCCCGAC CTCCCGGCGC TTCAGTCCGA CTGGCCCCGA GCCCCCGCTT
    CCCCCTCATC ACCACCCGTC ACCACCCCAC CACGGCTCTC CCCGCTCCGG CCGACATGCA
    CACTGGGGCC CCGAGCCTGC AGAGAGCGTG GAGGGTGACG GCTTCTATGA CGACCAAGAC
    TATGAGTTCA ATCACCACGA GCCTCCATCC TACGAGCAAA GCCCTGTGCA GGGCAACCCT
    CACCCCCATT CCCCTAGGAC TTCCCGCCAC AACACCCCTC CTCAGGGCCA AGCGCACCCA
    CGGCGCATGC CAAATGGCTA CCGTTCGTCT TCGCCCTCTC CACACCACCA CGCGCCCGCT
    CACCCTGGAA CTCATAAACC TCCTCATCCC AGAGGGCCCA GAAAGGGCCT CCATGAACCC
    TACAGTGAGA CTGACGAGGA TGACTGGTGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009297866.1
    CDS1510..8442
    Translation

    Target ORF information:

    RefSeq Version XM_009299591.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009299591.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACAGGAAGC AGCAAAGGGA AAGACATCAG TACTATCAAG
    TCTCTACGTG TGCTTCGAGT ACTGCGGCCG CTGAAAACCA TCAAACGTCT GCCAAAACTG
    AAGGCTGTCT TTGACTGTGT GGTGAACTCT CTGAAGAATG TGTTGAACAT CCTCATCGTC
    TACATGCTCT TCATGTTCAT CTTCGCTGTG GTGGCTGTGC AGCTCTTTAA GGGACGTTTC
    TTTTACTGCA CTGATGAATC CAAAGAGTTT GAGCGCGACT GCAGGGGCGA GTATCTGGTC
    TATGAAAGAG ATAACGAAGT GAGGTCACAG AAGCGGGAAT GGAAGAAATA CGATTTCCAC
    TACGACAACG TGCTCTGGGC CCTTCTCACC CTCTTCACCG TTTCTACGGG AGAGGGGTGG
    CCGCAAGTGC TAAAACATTC AGTAGATGCG ACCTACGAGA ATCAGGGCCC GAGCCCAGGT
    TACCGGATGG AAATGTCCAT CTTTTACGTG GTGTACTTCG TGGTCTTCCC CTTCTTCTTC
    GTCAACATCT TCGTGGCTCT TATCATCATT ACTTTCCAGG AGCAAGGAGA CAAAATGATG
    GAGGACTATA GCCTGGAAAA GAATGAGAGA GCCTGCATAG ACTTTGCCAT CAACGCTAAG
    CCGCTCACAC GGCACATGCC CCAGAACAAG CAGACCTTTC AGTACCGCAT GTGGGAGTTT
    GTGGTGTCTC CTCCATTTGA ATACACCATC ATGGCCTTGA TCGCGCTCAA CACTATTGTC
    CTTATGATGA AGTATGATGG AGCATCGCTT ACTTATGAAG ATGTGCTAAA ATACCTGAAC
    ATAGTGTTCA CCTCTCTATT CTCCATGGAG TGCATATTGA AGATCATAGC CTTCGGAGCA
    TTGAACTACT TCAAAGATGC CTGGAACATC TTCGACTGTG TGACTGTACT TGGCAGCATC
    ACTGATATTT TAGTCACAGA GCTGGGGAAC AACTTCATAA ATCTGAGTTT TCTAAGGCTC
    TTCAGAGCTG CTCGTCTCAT TAAGTTGCTC AGGCAGGGTG AAACCATACG CATCCTGCTC
    TGGACTTTTG TCCAGTCTTT CAAGGCTCTG CCGTATGTGT GCCTACTCAT CGCCATGCTG
    TTCTTCATCT ACGCCATCAT TGGCATGCAG CTGTTTGGGA ACATTAAGAT CGAGGAGAAC
    AGTGACAGTG CCATTACCCA GCACAACAAC TTCAGGACTT TTTTCCAGGC CCTAATGCTG
    CTCTTTAGGA GTGCTACAGG TGAGGCATGG CATGACATCA TGCTGTCATG TCTGGGAAAG
    AAGCCGTGTG ATATTCTCTC TGATAACCCG AAGCCAGAAT GCGGGAGCGA GTTTGCCTAC
    CTCTACTTTG TCTCCTTCAT CTTCCTCTGT TCATTTCTGA TGCTGAATCT GTTCGTGGCT
    GTGATCATGG ATAATTTTGA ATACCTGACG CGTGACTCCT CTATCCTGGG ACCCCATCAC
    CTGGACGAGT ATGTGAGGAT CTGGGCAGAA TATGATCCTG CTGCTTGCGG GCGCATTCAT
    TATAAGGATA TGTACAGTTT ATTGCGAGTT ATCGACCCCC CTCTGGGCTT AGGCAAGAAA
    TGTCCTCATA GGGTGGCCTG CAAGAGACTG TTGCGGATGG ATCTGCCGGT TGCCGATGAC
    AACACAGTGC ACTTTAATTC TACCCTTATG GCCTTAATCC GAACCGCTCT GGACATCAAG
    ATCGCCAAGG GAGGTGTAGA TAAGCACCAG ATGGATGCTG AATTAAGGAA GGAGATGATG
    GCGATATGGC CTAATCTCTC TCAGAAGAAC CTGGACCTGC TGGTGACTCC TCACAAGTCA
    GCGACAGACC TGACAGTAGG AAAGATCTAC GCTGCCATGA TGATCATGGA GTATTATCGG
    CAAAGTAAAG CCAAACGGAC ACAAGCTCTC CATGATGAGC AGAATCGAAC GCCATTAATG
    TTTCAGCGTC TGGAGCCGCC ATCTCCTAGC CAGGATGTGG GACCAGGACT CACTGGCCTT
    CCTGACACAC AAATGCACCC TGCAAATCAT CTGCCTGTTG ATGAAAGGAT CCCAGAGAGC
    CAATCATGGG TGACAGCGCG AGCACAGGAG ATTTCTCAAA AAGCAGGCAA CTGGAGTCCA
    GAGGGCCAAC ATCCAGATGA CACCACAGAC AACAGAAGGC AGTCTCAGCC TATCACAGAT
    AACAGTCCTA TGAAGCGCTC GGCTTCATCT TTGGGACACG GGCGTGCAGG ACGCTCCATG
    CGAGGTGAGG ATTATGCCAT GGAGAGGGTT ATCCCAGAAG AGGGTCACAG GCATGGGCAT
    CGCCACCGAG ACCGTAGTCA CCGGGCGTCA GAACGCTCCC TGAGCCGATA CACTGATGCT
    GACACAGGTC TTGGAACTGA CCTCAGCACA ACCACACAAT CAGGAGACCT GCCATCCAAG
    GAGAGAGACC GGGGCCGAGC CAAAGACCGC AAGCACCATC ATCATCATCA TCACCACCAC
    GGCTCTTTAG ATAAGGAGCA CTACGGTCAT GAGAGAGACA GAGGAGAGTA CGGCCACAGA
    CAGTCCAGAG AGAGAGACCG CCGCTGGTCC CGCTCACCCA GTGAGGGCAG AGAGTGTCTG
    ACTCACAGAC AGGGCAGTAG TTCAGTAAGC GGCAGTCCAG TCCCGTCCAC CTCCGGGACC
    AGCACCCCCC GCCGTGGCCG CCGTCAGCTC CCCCAAACCC CTGCTACACC CCGGCCACAT
    GTCACCTACT CACCTGTGGT CCGTAAGCCC ATCAGCAGTA CCCCCCCTCC TGGCCAGCAG
    AGCCGACTCC CTACCCCGAC CTCCCGGCGC TTCAGTCCGA CTGGCCCCGA GCCCCCGCTT
    CCCCCTCATC ACCACCCGTC ACCACCCCAC CACGGCTCTC CCCGCTCCGG CCGACATGCA
    CACTGGGGCC CCGAGCCTGC AGAGAGCGTG GAGGGTGACG GCTTCTATGA CGACCAAGAC
    TATGAGTTCA ATCACCACGA GCCTCCATCC TACGAGCAAA GCCCTGTGCA GGGCAACCCT
    CACCCCCATT CCCCTAGGAC TTCCCGCCAC AACACCCCTC CTCAGGGCCA AGCGCACCCA
    CGGCGCATGC CAAATGGCTA CCGTTCGTCT TCGCCCTCTC CACACCACCA CGCGCCCGCT
    CACCCTGGAA CTCATAAACC TCCTCATCCC AGAGGGCCCA GAAAGGGCCT CCATGAACCC
    TACAGTGAGA CTGACGAGGA TGACTGGTGC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43667
    Clone ID Related Accession (Same CDS sequence) XM_009299592.2
    Accession Version XM_009299592.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4704bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X20
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299592.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGT TTTTTTGTTA TAATCAACCT
    TTATTTGGAT CCAGAGTTTG TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009297867.1
    CDS1539..6242
    Translation

    Target ORF information:

    RefSeq Version XM_009299592.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009299592.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGT TTTTTTGTTA TAATCAACCT
    TTATTTGGAT CCAGAGTTTG TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43668
    Clone ID Related Accession (Same CDS sequence) XM_009299593.2 , XM_009299593.3
    Accession Version XM_009299593.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6882bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X21
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299593.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCTAAGGA GCAGCAGAAG AACCACAAAG GTTGTAAGTC GGTATGGGAG
    CAGCGCACTA GTGAGTTAAG ACGACAGACC CTCGTGAACA GCAGAGAGGC TCTTTATAAT
    GAGTTGGACC CTGAAGACCG TTGGAAGGTC TCATACTCCC GTCACATACG TCCCGACATG
    AAAACTCACC TGGACCGGCC GTTAGTCGTG GACCCTCAGG AGAACCGCAA TAACAACACT
    AACAAAACCC GTCCGGGTGA CGGCCAACCT CAGCATTCCC ATCAGCTCCT ACACAAACAG
    CCCAACTTCA ATGAGGCCGA GACTGGAGGA GGTGGAGGGT CTGAGTCCTG GCCCGCACTG
    GAAAGAGGAG ACTCAACAGG GTCCCGCCGC AGTCTTTTGG GCTCTGAGCA CTATGAGGAG
    GGTAGAGAGT CCCACCGCAG ACACCAGCAC AGCCAGCACT GCAGTCAGGC CCGTCAACAT
    CGCAAAGCTG CAGAGGGCGG TGGAGAGCCT GGAGGGAGGA GACACAGGAG TAAGGCCAAG
    GAAAGAGGAC CTGAGTGTGA GCATTCAGAT GGAGGGGAGC GGAAACACAG ACGGCATCGG
    CATGAGGGTG AGGGGAGGAA AGAGAGAGGG GTACGACACC GAAACAGGAG GGATGGCCAT
    GCAAGTGGCC CGACTCTCTC TACAACCCGT CCCATTCAGA AGACTCTGTC CAGGCAGGAC
    AGTCAGTACA GCGAAGACCT GGACAATGCC ATGAACAACA AACTTGCTAC CCAGCCACAT
    GATAGTCTGC TTAATCTGGC CAACACTGCC CACTCTGCCG GCCTGGCACA TACTGGTACA
    GAGAGCAGCC TCATCCTCAC CAACCCTTCC TCTACCATTG CAAATGTTAC TAGCATCGGT
    CACCTGGGTA TGAAACCTGA GTACACTGCC GTGGACATTC CTCCCATGTT CCCCTCCAGT
    AATGCCATTC TACAGGTCAA TAAGAATGCC AACACGGAGC CTCTGCCAAA GAAGGAAGAC
    ACAAAGGGTG ACGATGATGA TGATGAGAAA GATGACGGAG GGCCTAAACC CATGCCACCC
    TACACCTCAA TGTTCATTCT GACCACCACC AACCCATTTC GGAGATTGTG TCACTATATC
    GTCACACTGA GATATTTTGA GATGTGTATT CTACTGGTTA TTGCTATGAG CAGTATTGCC
    CTGGCTGCTG AAGACCCTGT CTGGCCTGAA TCTCCCCGCA ACAATGTCCT ACGATACTTT
    GACTATGTGT TCACTGGTGT GTTCACCTTT GAGATGCTCA TTAAGATGGT AGATTTAGGT
    CTTGTCCTGC ATCAAGGCTC ATACTTTCGT GATCTGTGGA ACATCCTGGA CTTTATAGTG
    GTTAGTGGTG CCCTGGTGGC GTTCGCCTTC ACAGGAAGCA GCAAAGGGAA AGACATCAGT
    ACTATCAAGT CTCTACGTGT GCTTCGAGTA CTGCGGCCGC TGAAAACCAT CAAACGTCTG
    CCAAAACTGA AGGCTGTCTT TGACTGTGTG GTGAACTCTC TGAAGAATGT GTTGAACATC
    CTCATCGTCT ACATGCTCTT CATGTTCATC TTCGCTGTGG TGGCTGTGCA GCTCTTTAAG
    GGACGTTTCT TTTACTGCAC TGATGAATCC AAAGAGTTTG AGCGCGACTG CAGGGGCGAG
    TATCTGGTCT ATGAAAGAGA TAACGAAGTG AGGTCACAGA AGCGGGAATG GAAGAAATAC
    GATTTCCACT ACGACAACGT GCTCTGGGCC CTTCTCACCC TCTTCACCGT TTCTACGGGA
    GAGGGGTGGC CGCAAGTGCT AAAACATTCA GTAGATGCGA CCTACGAGAA TCAGGGCCCG
    AGCCCAGGTT ACCGGATGGA AATGTCCATC TTTTACGTGG TGTACTTCGT GGTCTTCCCC
    TTCTTCTTCG TCAACATCTT CGTGGCTCTT ATCATCATTA CTTTCCAGGA GCAAGGAGAC
    AAAATGATGG AGGACTATAG CCTGGAAAAG AATGAGAGAG CCTGCATAGA CTTTGCCATC
    AACGCTAAGC CGCTCACACG GCACATGCCC CAGAACAAGC AGACCTTTCA GTACCGCATG
    TGGGAGTTTG TGGTGTCTCC TCCATTTGAA TACACCATCA TGGCCTTGAT CGCGCTCAAC
    ACTATTGTCC TTATGATGAA GTATGATGGA GCATCGCTTA CTTATGAAGA TGTGCTAAAA
    TACCTGAACA TAGTGTTCAC CTCTCTATTC TCCATGGAGT GCATATTGAA GATCATAGCC
    TTCGGAGCAT TGAACTACTT CAAAGATGCC TGGAACATCT TCGACTGTGT GACTGTACTT
    GGCAGCATCA CTGATATTTT AGTCACAGAG CTGGGGAACA ACTTCATAAA TCTGAGTTTT
    CTAAGGCTCT TCAGAGCTGC TCGTCTCATT AAGTTGCTCA GGCAGGGTGA AACCATACGC
    ATCCTGCTCT GGACTTTTGT CCAGTCTTTC AAGGCTCTGC CGTATGTGTG CCTACTCATC
    GCCATGCTGT TCTTCATCTA CGCCATCATT GGCATGCAGC TGTTTGGGAA CATTAAGATC
    GAGGAGAACA GTGACAGTGC CATTACCCAG CACAACAACT TCAGGACTTT TTTCCAGGCC
    CTAATGCTGC TCTTTAGGAG TGCTACAGGT GAGGCATGGC ATGACATCAT GCTGTCATGT
    CTGGGAAAGA AGCCGTGTGA TATTCTCTCT GATAACCCGA AGCCAGAATG CGGGAGCGAG
    TTTGCCTACC TCTACTTTGT CTCCTTCATC TTCCTCTGTT CATTTCTGAT GCTGAATCTG
    TTCGTGGCTG TGATCATGGA TAATTTTGAA TACCTGACGC GTGACTCCTC TATCCTGGGA
    CCCCATCACC TGGACGAGTA TGTGAGGATC TGGGCAGAAT ATGATCCTGC TGCTTGCGGG
    CGCATTCATT ATAAGGATAT GTACAGTTTA TTGCGAGTTA TCGACCCCCC TCTGGGCTTA
    GGCAAGAAAT GTCCTCATAG GGTGGCCTGC AAGAGACTGT TGCGGATGGA TCTGCCGGTT
    GCCGATGACA ACACAGTGCA CTTTAATTCT ACCCTTATGG CCTTAATCCG AACCGCTCTG
    GACATCAAGA TCGCCAAGGG AGGTGTAGAT AAGCACCAGA TGGATGCTGA ATTAAGGAAG
    GAGATGATGG CGATATGGCC TAATCTCTCT CAGAAGAACC TGGACCTGCT GGTGACTCCT
    CACAAGTCAG CGACAGACCT GACAGTAGGA AAGATCTACG CTGCCATGAT GATCATGGAG
    TATTATCGGC AAAGTAAAGC CAAACGGACA CAAGCTCTCC ATGATGAGCA GAATCGAACG
    CCATTAATGT TTCAGCGTCT GGAGCCGCCA TCTCCTAGCC AGGATGTGGG ACCAGGACTC
    ACTGGCCTTC CTGACACACA AATGCACCCT GCAAATCATC TGCCTGTTGA TGAAAGGATC
    CCAGAGAGCC AATCATGGGT GACAGCGCGA GCACAGGAGA TTTCTCAAAA AGCAGGCAAC
    TGGAGTCCAG AGGGCCAACA TCCAGATGAC ACCACAGACA ACAGAAGGCA GTCTCAGCCT
    ATCACAGATA ACAGTCCTAT GAAGCGCTCG GCTTCATCTT TGGGACACGG GCGTGCAGGA
    CGCTCCATGC GAGGTGAGGA TTATGCCATG GAGAGGGTTA TCCCAGAAGA GGGTCACAGG
    CATGGGCATC GCCACCGAGA CCGTAGTCAC CGGGCGTCAG AACGCTCCCT GAGCCGATAC
    ACTGATGCTG ACACAGGTCT TGGAACTGAC CTCAGCACAA CCACACAATC AGGAGACCTG
    CCATCCAAGG AGAGAGACCG GGGCCGAGCC AAAGACCGCA AGCACCATCA TCATCATCAT
    CACCACCACG GCTCTTTAGA TAAGGAGCAC TACGGTCATG AGAGAGACAG AGGAGAGTAC
    GGCCACAGAC AGTCCAGAGA GAGAGACCGC CGCTGGTCCC GCTCACCCAG TGAGGGCAGA
    GAGTGTCTGA CTCACAGACA GGGCAGTAGT TCAGTAAGCG GCAGTCCAGT CCCGTCCACC
    TCCGGGACCA GCACCCCCCG CCGTGGCCGC CGTCAGCTCC CCCAAACCCC TGCTACACCC
    CGGCCACATG TCACCTACTC ACCTGTGGTC CGTAAGCCCA TCAGCAGTAC CCCCCCTCCT
    GGCCAGCAGA GCCGACTCCC TACCCCGACC TCCCGGCGCT TCAGTCCGAC TGGCCCCGAG
    CCCCCGCTTC CCCCTCATCA CCACCCGTCA CCACCCCACC ACGGCTCTCC CCGCTCCGGC
    CGACATGCAC ACTGGGGCCC CGAGCCTGCA GAGAGCGTGG AGGGTGACGG CTTCTATGAC
    GACCAAGACT ATGAGTTCAA TCACCACGAG CCTCCATCCT ACGAGCAAAG CCCTGTGCAG
    GGCAACCCTC ACCCCCATTC CCCTAGGACT TCCCGCCACA ACACCCCTCC TCAGGGCCAA
    GCGCACCCAC GGCGCATGCC AAATGGCTAC CGTTCGTCTT CGCCCTCTCC ACACCACCAC
    GCGCCCGCTC ACCCTGGAAC TCATAAACCT CCTCATCCCA GAGGGCCCAG AAAGGGCCTC
    CATGAACCCT ACAGTGAGAC TGACGAGGAT GACTGGTGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009297868.1
    CDS1467..8348
    Translation

    Target ORF information:

    RefSeq Version XM_009299593.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009299593.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCTAAGGA GCAGCAGAAG AACCACAAAG GTTGTAAGTC GGTATGGGAG
    CAGCGCACTA GTGAGTTAAG ACGACAGACC CTCGTGAACA GCAGAGAGGC TCTTTATAAT
    GAGTTGGACC CTGAAGACCG TTGGAAGGTC TCATACTCCC GTCACATACG TCCCGACATG
    AAAACTCACC TGGACCGGCC GTTAGTCGTG GACCCTCAGG AGAACCGCAA TAACAACACT
    AACAAAACCC GTCCGGGTGA CGGCCAACCT CAGCATTCCC ATCAGCTCCT ACACAAACAG
    CCCAACTTCA ATGAGGCCGA GACTGGAGGA GGTGGAGGGT CTGAGTCCTG GCCCGCACTG
    GAAAGAGGAG ACTCAACAGG GTCCCGCCGC AGTCTTTTGG GCTCTGAGCA CTATGAGGAG
    GGTAGAGAGT CCCACCGCAG ACACCAGCAC AGCCAGCACT GCAGTCAGGC CCGTCAACAT
    CGCAAAGCTG CAGAGGGCGG TGGAGAGCCT GGAGGGAGGA GACACAGGAG TAAGGCCAAG
    GAAAGAGGAC CTGAGTGTGA GCATTCAGAT GGAGGGGAGC GGAAACACAG ACGGCATCGG
    CATGAGGGTG AGGGGAGGAA AGAGAGAGGG GTACGACACC GAAACAGGAG GGATGGCCAT
    GCAAGTGGCC CGACTCTCTC TACAACCCGT CCCATTCAGA AGACTCTGTC CAGGCAGGAC
    AGTCAGTACA GCGAAGACCT GGACAATGCC ATGAACAACA AACTTGCTAC CCAGCCACAT
    GATAGTCTGC TTAATCTGGC CAACACTGCC CACTCTGCCG GCCTGGCACA TACTGGTACA
    GAGAGCAGCC TCATCCTCAC CAACCCTTCC TCTACCATTG CAAATGTTAC TAGCATCGGT
    CACCTGGGTA TGAAACCTGA GTACACTGCC GTGGACATTC CTCCCATGTT CCCCTCCAGT
    AATGCCATTC TACAGGTCAA TAAGAATGCC AACACGGAGC CTCTGCCAAA GAAGGAAGAC
    ACAAAGGGTG ACGATGATGA TGATGAGAAA GATGACGGAG GGCCTAAACC CATGCCACCC
    TACACCTCAA TGTTCATTCT GACCACCACC AACCCATTTC GGAGATTGTG TCACTATATC
    GTCACACTGA GATATTTTGA GATGTGTATT CTACTGGTTA TTGCTATGAG CAGTATTGCC
    CTGGCTGCTG AAGACCCTGT CTGGCCTGAA TCTCCCCGCA ACAATGTCCT ACGATACTTT
    GACTATGTGT TCACTGGTGT GTTCACCTTT GAGATGCTCA TTAAGATGGT AGATTTAGGT
    CTTGTCCTGC ATCAAGGCTC ATACTTTCGT GATCTGTGGA ACATCCTGGA CTTTATAGTG
    GTTAGTGGTG CCCTGGTGGC GTTCGCCTTC ACAGGAAGCA GCAAAGGGAA AGACATCAGT
    ACTATCAAGT CTCTACGTGT GCTTCGAGTA CTGCGGCCGC TGAAAACCAT CAAACGTCTG
    CCAAAACTGA AGGCTGTCTT TGACTGTGTG GTGAACTCTC TGAAGAATGT GTTGAACATC
    CTCATCGTCT ACATGCTCTT CATGTTCATC TTCGCTGTGG TGGCTGTGCA GCTCTTTAAG
    GGACGTTTCT TTTACTGCAC TGATGAATCC AAAGAGTTTG AGCGCGACTG CAGGGGCGAG
    TATCTGGTCT ATGAAAGAGA TAACGAAGTG AGGTCACAGA AGCGGGAATG GAAGAAATAC
    GATTTCCACT ACGACAACGT GCTCTGGGCC CTTCTCACCC TCTTCACCGT TTCTACGGGA
    GAGGGGTGGC CGCAAGTGCT AAAACATTCA GTAGATGCGA CCTACGAGAA TCAGGGCCCG
    AGCCCAGGTT ACCGGATGGA AATGTCCATC TTTTACGTGG TGTACTTCGT GGTCTTCCCC
    TTCTTCTTCG TCAACATCTT CGTGGCTCTT ATCATCATTA CTTTCCAGGA GCAAGGAGAC
    AAAATGATGG AGGACTATAG CCTGGAAAAG AATGAGAGAG CCTGCATAGA CTTTGCCATC
    AACGCTAAGC CGCTCACACG GCACATGCCC CAGAACAAGC AGACCTTTCA GTACCGCATG
    TGGGAGTTTG TGGTGTCTCC TCCATTTGAA TACACCATCA TGGCCTTGAT CGCGCTCAAC
    ACTATTGTCC TTATGATGAA GTATGATGGA GCATCGCTTA CTTATGAAGA TGTGCTAAAA
    TACCTGAACA TAGTGTTCAC CTCTCTATTC TCCATGGAGT GCATATTGAA GATCATAGCC
    TTCGGAGCAT TGAACTACTT CAAAGATGCC TGGAACATCT TCGACTGTGT GACTGTACTT
    GGCAGCATCA CTGATATTTT AGTCACAGAG CTGGGGAACA ACTTCATAAA TCTGAGTTTT
    CTAAGGCTCT TCAGAGCTGC TCGTCTCATT AAGTTGCTCA GGCAGGGTGA AACCATACGC
    ATCCTGCTCT GGACTTTTGT CCAGTCTTTC AAGGCTCTGC CGTATGTGTG CCTACTCATC
    GCCATGCTGT TCTTCATCTA CGCCATCATT GGCATGCAGC TGTTTGGGAA CATTAAGATC
    GAGGAGAACA GTGACAGTGC CATTACCCAG CACAACAACT TCAGGACTTT TTTCCAGGCC
    CTAATGCTGC TCTTTAGGAG TGCTACAGGT GAGGCATGGC ATGACATCAT GCTGTCATGT
    CTGGGAAAGA AGCCGTGTGA TATTCTCTCT GATAACCCGA AGCCAGAATG CGGGAGCGAG
    TTTGCCTACC TCTACTTTGT CTCCTTCATC TTCCTCTGTT CATTTCTGAT GCTGAATCTG
    TTCGTGGCTG TGATCATGGA TAATTTTGAA TACCTGACGC GTGACTCCTC TATCCTGGGA
    CCCCATCACC TGGACGAGTA TGTGAGGATC TGGGCAGAAT ATGATCCTGC TGCTTGCGGG
    CGCATTCATT ATAAGGATAT GTACAGTTTA TTGCGAGTTA TCGACCCCCC TCTGGGCTTA
    GGCAAGAAAT GTCCTCATAG GGTGGCCTGC AAGAGACTGT TGCGGATGGA TCTGCCGGTT
    GCCGATGACA ACACAGTGCA CTTTAATTCT ACCCTTATGG CCTTAATCCG AACCGCTCTG
    GACATCAAGA TCGCCAAGGG AGGTGTAGAT AAGCACCAGA TGGATGCTGA ATTAAGGAAG
    GAGATGATGG CGATATGGCC TAATCTCTCT CAGAAGAACC TGGACCTGCT GGTGACTCCT
    CACAAGTCAG CGACAGACCT GACAGTAGGA AAGATCTACG CTGCCATGAT GATCATGGAG
    TATTATCGGC AAAGTAAAGC CAAACGGACA CAAGCTCTCC ATGATGAGCA GAATCGAACG
    CCATTAATGT TTCAGCGTCT GGAGCCGCCA TCTCCTAGCC AGGATGTGGG ACCAGGACTC
    ACTGGCCTTC CTGACACACA AATGCACCCT GCAAATCATC TGCCTGTTGA TGAAAGGATC
    CCAGAGAGCC AATCATGGGT GACAGCGCGA GCACAGGAGA TTTCTCAAAA AGCAGGCAAC
    TGGAGTCCAG AGGGCCAACA TCCAGATGAC ACCACAGACA ACAGAAGGCA GTCTCAGCCT
    ATCACAGATA ACAGTCCTAT GAAGCGCTCG GCTTCATCTT TGGGACACGG GCGTGCAGGA
    CGCTCCATGC GAGGTGAGGA TTATGCCATG GAGAGGGTTA TCCCAGAAGA GGGTCACAGG
    CATGGGCATC GCCACCGAGA CCGTAGTCAC CGGGCGTCAG AACGCTCCCT GAGCCGATAC
    ACTGATGCTG ACACAGGTCT TGGAACTGAC CTCAGCACAA CCACACAATC AGGAGACCTG
    CCATCCAAGG AGAGAGACCG GGGCCGAGCC AAAGACCGCA AGCACCATCA TCATCATCAT
    CACCACCACG GCTCTTTAGA TAAGGAGCAC TACGGTCATG AGAGAGACAG AGGAGAGTAC
    GGCCACAGAC AGTCCAGAGA GAGAGACCGC CGCTGGTCCC GCTCACCCAG TGAGGGCAGA
    GAGTGTCTGA CTCACAGACA GGGCAGTAGT TCAGTAAGCG GCAGTCCAGT CCCGTCCACC
    TCCGGGACCA GCACCCCCCG CCGTGGCCGC CGTCAGCTCC CCCAAACCCC TGCTACACCC
    CGGCCACATG TCACCTACTC ACCTGTGGTC CGTAAGCCCA TCAGCAGTAC CCCCCCTCCT
    GGCCAGCAGA GCCGACTCCC TACCCCGACC TCCCGGCGCT TCAGTCCGAC TGGCCCCGAG
    CCCCCGCTTC CCCCTCATCA CCACCCGTCA CCACCCCACC ACGGCTCTCC CCGCTCCGGC
    CGACATGCAC ACTGGGGCCC CGAGCCTGCA GAGAGCGTGG AGGGTGACGG CTTCTATGAC
    GACCAAGACT ATGAGTTCAA TCACCACGAG CCTCCATCCT ACGAGCAAAG CCCTGTGCAG
    GGCAACCCTC ACCCCCATTC CCCTAGGACT TCCCGCCACA ACACCCCTCC TCAGGGCCAA
    GCGCACCCAC GGCGCATGCC AAATGGCTAC CGTTCGTCTT CGCCCTCTCC ACACCACCAC
    GCGCCCGCTC ACCCTGGAAC TCATAAACCT CCTCATCCCA GAGGGCCCAG AAAGGGCCTC
    CATGAACCCT ACAGTGAGAC TGACGAGGAT GACTGGTGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43668
    Clone ID Related Accession (Same CDS sequence) XM_009299593.2 , XM_009299593.3
    Accession Version XM_009299593.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6882bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X26
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009299593.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCTAAGGA GCAGCAGAAG AACCACAAAG GTTGTAAGTC GGTATGGGAG
    CAGCGCACTA GTGAGTTAAG ACGACAGACC CTCGTGAACA GCAGAGAGGC TCTTTATAAT
    GAGTTGGACC CTGAAGACCG TTGGAAGGTC TCATACTCCC GTCACATACG TCCCGACATG
    AAAACTCACC TGGACCGGCC GTTAGTCGTG GACCCTCAGG AGAACCGCAA TAACAACACT
    AACAAAACCC GTCCGGGTGA CGGCCAACCT CAGCATTCCC ATCAGCTCCT ACACAAACAG
    CCCAACTTCA ATGAGGCCGA GACTGGAGGA GGTGGAGGGT CTGAGTCCTG GCCCGCACTG
    GAAAGAGGAG ACTCAACAGG GTCCCGCCGC AGTCTTTTGG GCTCTGAGCA CTATGAGGAG
    GGTAGAGAGT CCCACCGCAG ACACCAGCAC AGCCAGCACT GCAGTCAGGC CCGTCAACAT
    CGCAAAGCTG CAGAGGGCGG TGGAGAGCCT GGAGGGAGGA GACACAGGAG TAAGGCCAAG
    GAAAGAGGAC CTGAGTGTGA GCATTCAGAT GGAGGGGAGC GGAAACACAG ACGGCATCGG
    CATGAGGGTG AGGGGAGGAA AGAGAGAGGG GTACGACACC GAAACAGGAG GGATGGCCAT
    GCAAGTGGCC CGACTCTCTC TACAACCCGT CCCATTCAGA AGACTCTGTC CAGGCAGGAC
    AGTCAGTACA GCGAAGACCT GGACAATGCC ATGAACAACA AACTTGCTAC CCAGCCACAT
    GATAGTCTGC TTAATCTGGC CAACACTGCC CACTCTGCCG GCCTGGCACA TACTGGTACA
    GAGAGCAGCC TCATCCTCAC CAACCCTTCC TCTACCATTG CAAATGTTAC TAGCATCGGT
    CACCTGGGTA TGAAACCTGA GTACACTGCC GTGGACATTC CTCCCATGTT CCCCTCCAGT
    AATGCCATTC TACAGGTCAA TAAGAATGCC AACACGGAGC CTCTGCCAAA GAAGGAAGAC
    ACAAAGGGTG ACGATGATGA TGATGAGAAA GATGACGGAG GGCCTAAACC CATGCCACCC
    TACACCTCAA TGTTCATTCT GACCACCACC AACCCATTTC GGAGATTGTG TCACTATATC
    GTCACACTGA GATATTTTGA GATGTGTATT CTACTGGTTA TTGCTATGAG CAGTATTGCC
    CTGGCTGCTG AAGACCCTGT CTGGCCTGAA TCTCCCCGCA ACAATGTCCT ACGATACTTT
    GACTATGTGT TCACTGGTGT GTTCACCTTT GAGATGCTCA TTAAGATGGT AGATTTAGGT
    CTTGTCCTGC ATCAAGGCTC ATACTTTCGT GATCTGTGGA ACATCCTGGA CTTTATAGTG
    GTTAGTGGTG CCCTGGTGGC GTTCGCCTTC ACAGGAAGCA GCAAAGGGAA AGACATCAGT
    ACTATCAAGT CTCTACGTGT GCTTCGAGTA CTGCGGCCGC TGAAAACCAT CAAACGTCTG
    CCAAAACTGA AGGCTGTCTT TGACTGTGTG GTGAACTCTC TGAAGAATGT GTTGAACATC
    CTCATCGTCT ACATGCTCTT CATGTTCATC TTCGCTGTGG TGGCTGTGCA GCTCTTTAAG
    GGACGTTTCT TTTACTGCAC TGATGAATCC AAAGAGTTTG AGCGCGACTG CAGGGGCGAG
    TATCTGGTCT ATGAAAGAGA TAACGAAGTG AGGTCACAGA AGCGGGAATG GAAGAAATAC
    GATTTCCACT ACGACAACGT GCTCTGGGCC CTTCTCACCC TCTTCACCGT TTCTACGGGA
    GAGGGGTGGC CGCAAGTGCT AAAACATTCA GTAGATGCGA CCTACGAGAA TCAGGGCCCG
    AGCCCAGGTT ACCGGATGGA AATGTCCATC TTTTACGTGG TGTACTTCGT GGTCTTCCCC
    TTCTTCTTCG TCAACATCTT CGTGGCTCTT ATCATCATTA CTTTCCAGGA GCAAGGAGAC
    AAAATGATGG AGGACTATAG CCTGGAAAAG AATGAGAGAG CCTGCATAGA CTTTGCCATC
    AACGCTAAGC CGCTCACACG GCACATGCCC CAGAACAAGC AGACCTTTCA GTACCGCATG
    TGGGAGTTTG TGGTGTCTCC TCCATTTGAA TACACCATCA TGGCCTTGAT CGCGCTCAAC
    ACTATTGTCC TTATGATGAA GTATGATGGA GCATCGCTTA CTTATGAAGA TGTGCTAAAA
    TACCTGAACA TAGTGTTCAC CTCTCTATTC TCCATGGAGT GCATATTGAA GATCATAGCC
    TTCGGAGCAT TGAACTACTT CAAAGATGCC TGGAACATCT TCGACTGTGT GACTGTACTT
    GGCAGCATCA CTGATATTTT AGTCACAGAG CTGGGGAACA ACTTCATAAA TCTGAGTTTT
    CTAAGGCTCT TCAGAGCTGC TCGTCTCATT AAGTTGCTCA GGCAGGGTGA AACCATACGC
    ATCCTGCTCT GGACTTTTGT CCAGTCTTTC AAGGCTCTGC CGTATGTGTG CCTACTCATC
    GCCATGCTGT TCTTCATCTA CGCCATCATT GGCATGCAGC TGTTTGGGAA CATTAAGATC
    GAGGAGAACA GTGACAGTGC CATTACCCAG CACAACAACT TCAGGACTTT TTTCCAGGCC
    CTAATGCTGC TCTTTAGGAG TGCTACAGGT GAGGCATGGC ATGACATCAT GCTGTCATGT
    CTGGGAAAGA AGCCGTGTGA TATTCTCTCT GATAACCCGA AGCCAGAATG CGGGAGCGAG
    TTTGCCTACC TCTACTTTGT CTCCTTCATC TTCCTCTGTT CATTTCTGAT GCTGAATCTG
    TTCGTGGCTG TGATCATGGA TAATTTTGAA TACCTGACGC GTGACTCCTC TATCCTGGGA
    CCCCATCACC TGGACGAGTA TGTGAGGATC TGGGCAGAAT ATGATCCTGC TGCTTGCGGG
    CGCATTCATT ATAAGGATAT GTACAGTTTA TTGCGAGTTA TCGACCCCCC TCTGGGCTTA
    GGCAAGAAAT GTCCTCATAG GGTGGCCTGC AAGAGACTGT TGCGGATGGA TCTGCCGGTT
    GCCGATGACA ACACAGTGCA CTTTAATTCT ACCCTTATGG CCTTAATCCG AACCGCTCTG
    GACATCAAGA TCGCCAAGGG AGGTGTAGAT AAGCACCAGA TGGATGCTGA ATTAAGGAAG
    GAGATGATGG CGATATGGCC TAATCTCTCT CAGAAGAACC TGGACCTGCT GGTGACTCCT
    CACAAGTCAG CGACAGACCT GACAGTAGGA AAGATCTACG CTGCCATGAT GATCATGGAG
    TATTATCGGC AAAGTAAAGC CAAACGGACA CAAGCTCTCC ATGATGAGCA GAATCGAACG
    CCATTAATGT TTCAGCGTCT GGAGCCGCCA TCTCCTAGCC AGGATGTGGG ACCAGGACTC
    ACTGGCCTTC CTGACACACA AATGCACCCT GCAAATCATC TGCCTGTTGA TGAAAGGATC
    CCAGAGAGCC AATCATGGGT GACAGCGCGA GCACAGGAGA TTTCTCAAAA AGCAGGCAAC
    TGGAGTCCAG AGGGCCAACA TCCAGATGAC ACCACAGACA ACAGAAGGCA GTCTCAGCCT
    ATCACAGATA ACAGTCCTAT GAAGCGCTCG GCTTCATCTT TGGGACACGG GCGTGCAGGA
    CGCTCCATGC GAGGTGAGGA TTATGCCATG GAGAGGGTTA TCCCAGAAGA GGGTCACAGG
    CATGGGCATC GCCACCGAGA CCGTAGTCAC CGGGCGTCAG AACGCTCCCT GAGCCGATAC
    ACTGATGCTG ACACAGGTCT TGGAACTGAC CTCAGCACAA CCACACAATC AGGAGACCTG
    CCATCCAAGG AGAGAGACCG GGGCCGAGCC AAAGACCGCA AGCACCATCA TCATCATCAT
    CACCACCACG GCTCTTTAGA TAAGGAGCAC TACGGTCATG AGAGAGACAG AGGAGAGTAC
    GGCCACAGAC AGTCCAGAGA GAGAGACCGC CGCTGGTCCC GCTCACCCAG TGAGGGCAGA
    GAGTGTCTGA CTCACAGACA GGGCAGTAGT TCAGTAAGCG GCAGTCCAGT CCCGTCCACC
    TCCGGGACCA GCACCCCCCG CCGTGGCCGC CGTCAGCTCC CCCAAACCCC TGCTACACCC
    CGGCCACATG TCACCTACTC ACCTGTGGTC CGTAAGCCCA TCAGCAGTAC CCCCCCTCCT
    GGCCAGCAGA GCCGACTCCC TACCCCGACC TCCCGGCGCT TCAGTCCGAC TGGCCCCGAG
    CCCCCGCTTC CCCCTCATCA CCACCCGTCA CCACCCCACC ACGGCTCTCC CCGCTCCGGC
    CGACATGCAC ACTGGGGCCC CGAGCCTGCA GAGAGCGTGG AGGGTGACGG CTTCTATGAC
    GACCAAGACT ATGAGTTCAA TCACCACGAG CCTCCATCCT ACGAGCAAAG CCCTGTGCAG
    GGCAACCCTC ACCCCCATTC CCCTAGGACT TCCCGCCACA ACACCCCTCC TCAGGGCCAA
    GCGCACCCAC GGCGCATGCC AAATGGCTAC CGTTCGTCTT CGCCCTCTCC ACACCACCAC
    GCGCCCGCTC ACCCTGGAAC TCATAAACCT CCTCATCCCA GAGGGCCCAG AAAGGGCCTC
    CATGAACCCT ACAGTGAGAC TGACGAGGAT GACTGGTGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009297868.1
    CDS1507..8388
    Translation

    Target ORF information:

    RefSeq Version XM_009299593.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X26, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009299593.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCTAAGGA GCAGCAGAAG AACCACAAAG GTTGTAAGTC GGTATGGGAG
    CAGCGCACTA GTGAGTTAAG ACGACAGACC CTCGTGAACA GCAGAGAGGC TCTTTATAAT
    GAGTTGGACC CTGAAGACCG TTGGAAGGTC TCATACTCCC GTCACATACG TCCCGACATG
    AAAACTCACC TGGACCGGCC GTTAGTCGTG GACCCTCAGG AGAACCGCAA TAACAACACT
    AACAAAACCC GTCCGGGTGA CGGCCAACCT CAGCATTCCC ATCAGCTCCT ACACAAACAG
    CCCAACTTCA ATGAGGCCGA GACTGGAGGA GGTGGAGGGT CTGAGTCCTG GCCCGCACTG
    GAAAGAGGAG ACTCAACAGG GTCCCGCCGC AGTCTTTTGG GCTCTGAGCA CTATGAGGAG
    GGTAGAGAGT CCCACCGCAG ACACCAGCAC AGCCAGCACT GCAGTCAGGC CCGTCAACAT
    CGCAAAGCTG CAGAGGGCGG TGGAGAGCCT GGAGGGAGGA GACACAGGAG TAAGGCCAAG
    GAAAGAGGAC CTGAGTGTGA GCATTCAGAT GGAGGGGAGC GGAAACACAG ACGGCATCGG
    CATGAGGGTG AGGGGAGGAA AGAGAGAGGG GTACGACACC GAAACAGGAG GGATGGCCAT
    GCAAGTGGCC CGACTCTCTC TACAACCCGT CCCATTCAGA AGACTCTGTC CAGGCAGGAC
    AGTCAGTACA GCGAAGACCT GGACAATGCC ATGAACAACA AACTTGCTAC CCAGCCACAT
    GATAGTCTGC TTAATCTGGC CAACACTGCC CACTCTGCCG GCCTGGCACA TACTGGTACA
    GAGAGCAGCC TCATCCTCAC CAACCCTTCC TCTACCATTG CAAATGTTAC TAGCATCGGT
    CACCTGGGTA TGAAACCTGA GTACACTGCC GTGGACATTC CTCCCATGTT CCCCTCCAGT
    AATGCCATTC TACAGGTCAA TAAGAATGCC AACACGGAGC CTCTGCCAAA GAAGGAAGAC
    ACAAAGGGTG ACGATGATGA TGATGAGAAA GATGACGGAG GGCCTAAACC CATGCCACCC
    TACACCTCAA TGTTCATTCT GACCACCACC AACCCATTTC GGAGATTGTG TCACTATATC
    GTCACACTGA GATATTTTGA GATGTGTATT CTACTGGTTA TTGCTATGAG CAGTATTGCC
    CTGGCTGCTG AAGACCCTGT CTGGCCTGAA TCTCCCCGCA ACAATGTCCT ACGATACTTT
    GACTATGTGT TCACTGGTGT GTTCACCTTT GAGATGCTCA TTAAGATGGT AGATTTAGGT
    CTTGTCCTGC ATCAAGGCTC ATACTTTCGT GATCTGTGGA ACATCCTGGA CTTTATAGTG
    GTTAGTGGTG CCCTGGTGGC GTTCGCCTTC ACAGGAAGCA GCAAAGGGAA AGACATCAGT
    ACTATCAAGT CTCTACGTGT GCTTCGAGTA CTGCGGCCGC TGAAAACCAT CAAACGTCTG
    CCAAAACTGA AGGCTGTCTT TGACTGTGTG GTGAACTCTC TGAAGAATGT GTTGAACATC
    CTCATCGTCT ACATGCTCTT CATGTTCATC TTCGCTGTGG TGGCTGTGCA GCTCTTTAAG
    GGACGTTTCT TTTACTGCAC TGATGAATCC AAAGAGTTTG AGCGCGACTG CAGGGGCGAG
    TATCTGGTCT ATGAAAGAGA TAACGAAGTG AGGTCACAGA AGCGGGAATG GAAGAAATAC
    GATTTCCACT ACGACAACGT GCTCTGGGCC CTTCTCACCC TCTTCACCGT TTCTACGGGA
    GAGGGGTGGC CGCAAGTGCT AAAACATTCA GTAGATGCGA CCTACGAGAA TCAGGGCCCG
    AGCCCAGGTT ACCGGATGGA AATGTCCATC TTTTACGTGG TGTACTTCGT GGTCTTCCCC
    TTCTTCTTCG TCAACATCTT CGTGGCTCTT ATCATCATTA CTTTCCAGGA GCAAGGAGAC
    AAAATGATGG AGGACTATAG CCTGGAAAAG AATGAGAGAG CCTGCATAGA CTTTGCCATC
    AACGCTAAGC CGCTCACACG GCACATGCCC CAGAACAAGC AGACCTTTCA GTACCGCATG
    TGGGAGTTTG TGGTGTCTCC TCCATTTGAA TACACCATCA TGGCCTTGAT CGCGCTCAAC
    ACTATTGTCC TTATGATGAA GTATGATGGA GCATCGCTTA CTTATGAAGA TGTGCTAAAA
    TACCTGAACA TAGTGTTCAC CTCTCTATTC TCCATGGAGT GCATATTGAA GATCATAGCC
    TTCGGAGCAT TGAACTACTT CAAAGATGCC TGGAACATCT TCGACTGTGT GACTGTACTT
    GGCAGCATCA CTGATATTTT AGTCACAGAG CTGGGGAACA ACTTCATAAA TCTGAGTTTT
    CTAAGGCTCT TCAGAGCTGC TCGTCTCATT AAGTTGCTCA GGCAGGGTGA AACCATACGC
    ATCCTGCTCT GGACTTTTGT CCAGTCTTTC AAGGCTCTGC CGTATGTGTG CCTACTCATC
    GCCATGCTGT TCTTCATCTA CGCCATCATT GGCATGCAGC TGTTTGGGAA CATTAAGATC
    GAGGAGAACA GTGACAGTGC CATTACCCAG CACAACAACT TCAGGACTTT TTTCCAGGCC
    CTAATGCTGC TCTTTAGGAG TGCTACAGGT GAGGCATGGC ATGACATCAT GCTGTCATGT
    CTGGGAAAGA AGCCGTGTGA TATTCTCTCT GATAACCCGA AGCCAGAATG CGGGAGCGAG
    TTTGCCTACC TCTACTTTGT CTCCTTCATC TTCCTCTGTT CATTTCTGAT GCTGAATCTG
    TTCGTGGCTG TGATCATGGA TAATTTTGAA TACCTGACGC GTGACTCCTC TATCCTGGGA
    CCCCATCACC TGGACGAGTA TGTGAGGATC TGGGCAGAAT ATGATCCTGC TGCTTGCGGG
    CGCATTCATT ATAAGGATAT GTACAGTTTA TTGCGAGTTA TCGACCCCCC TCTGGGCTTA
    GGCAAGAAAT GTCCTCATAG GGTGGCCTGC AAGAGACTGT TGCGGATGGA TCTGCCGGTT
    GCCGATGACA ACACAGTGCA CTTTAATTCT ACCCTTATGG CCTTAATCCG AACCGCTCTG
    GACATCAAGA TCGCCAAGGG AGGTGTAGAT AAGCACCAGA TGGATGCTGA ATTAAGGAAG
    GAGATGATGG CGATATGGCC TAATCTCTCT CAGAAGAACC TGGACCTGCT GGTGACTCCT
    CACAAGTCAG CGACAGACCT GACAGTAGGA AAGATCTACG CTGCCATGAT GATCATGGAG
    TATTATCGGC AAAGTAAAGC CAAACGGACA CAAGCTCTCC ATGATGAGCA GAATCGAACG
    CCATTAATGT TTCAGCGTCT GGAGCCGCCA TCTCCTAGCC AGGATGTGGG ACCAGGACTC
    ACTGGCCTTC CTGACACACA AATGCACCCT GCAAATCATC TGCCTGTTGA TGAAAGGATC
    CCAGAGAGCC AATCATGGGT GACAGCGCGA GCACAGGAGA TTTCTCAAAA AGCAGGCAAC
    TGGAGTCCAG AGGGCCAACA TCCAGATGAC ACCACAGACA ACAGAAGGCA GTCTCAGCCT
    ATCACAGATA ACAGTCCTAT GAAGCGCTCG GCTTCATCTT TGGGACACGG GCGTGCAGGA
    CGCTCCATGC GAGGTGAGGA TTATGCCATG GAGAGGGTTA TCCCAGAAGA GGGTCACAGG
    CATGGGCATC GCCACCGAGA CCGTAGTCAC CGGGCGTCAG AACGCTCCCT GAGCCGATAC
    ACTGATGCTG ACACAGGTCT TGGAACTGAC CTCAGCACAA CCACACAATC AGGAGACCTG
    CCATCCAAGG AGAGAGACCG GGGCCGAGCC AAAGACCGCA AGCACCATCA TCATCATCAT
    CACCACCACG GCTCTTTAGA TAAGGAGCAC TACGGTCATG AGAGAGACAG AGGAGAGTAC
    GGCCACAGAC AGTCCAGAGA GAGAGACCGC CGCTGGTCCC GCTCACCCAG TGAGGGCAGA
    GAGTGTCTGA CTCACAGACA GGGCAGTAGT TCAGTAAGCG GCAGTCCAGT CCCGTCCACC
    TCCGGGACCA GCACCCCCCG CCGTGGCCGC CGTCAGCTCC CCCAAACCCC TGCTACACCC
    CGGCCACATG TCACCTACTC ACCTGTGGTC CGTAAGCCCA TCAGCAGTAC CCCCCCTCCT
    GGCCAGCAGA GCCGACTCCC TACCCCGACC TCCCGGCGCT TCAGTCCGAC TGGCCCCGAG
    CCCCCGCTTC CCCCTCATCA CCACCCGTCA CCACCCCACC ACGGCTCTCC CCGCTCCGGC
    CGACATGCAC ACTGGGGCCC CGAGCCTGCA GAGAGCGTGG AGGGTGACGG CTTCTATGAC
    GACCAAGACT ATGAGTTCAA TCACCACGAG CCTCCATCCT ACGAGCAAAG CCCTGTGCAG
    GGCAACCCTC ACCCCCATTC CCCTAGGACT TCCCGCCACA ACACCCCTCC TCAGGGCCAA
    GCGCACCCAC GGCGCATGCC AAATGGCTAC CGTTCGTCTT CGCCCTCTCC ACACCACCAC
    GCGCCCGCTC ACCCTGGAAC TCATAAACCT CCTCATCCCA GAGGGCCCAG AAAGGGCCTC
    CATGAACCCT ACAGTGAGAC TGACGAGGAT GACTGGTGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa58247
    Clone ID Related Accession (Same CDS sequence) XM_017354791.1
    Accession Version XM_017354791.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7074bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGTGGCAGAA TCAGTTACAG GGATATGTAT GAGATGCTCA GGCACATGTC TCCTCCGTTA
    GGCCTGGGGA AGAAGTGCCC TGCACGGGTT GCCTACAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT
    GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC
    CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC
    ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT
    GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT
    GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT
    GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT
    CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA
    AGGCAGTCTC AGACAGTAGA GATGAGAGAG ATGGGGCGGG ACGGGTACTC GGACACAGAG
    CAATATCTGC CGATGGAAGG CCATGGCAGG GCTGCTTCCA TGCCTCGACT CCCAGCAGAC
    AACCAACAGC CTATCACAGA TAACAGTCCT ATGAAGCGCT CGGCTTCATC TTTGGGACAC
    GGGCGTGCAG GACGCTCCAT GCGAGGTGAG GATTATGCCA TGGAGAGGGT TATCCCAGAA
    GAGGGTCACA GGCATGGGCA TCGCCACCGA GACCGTAGTC ACCGGGCGTC AGAACGCTCC
    CTGAGCCGAT ACACTGATGC TGACACAGGT CTTGGAACTG ACCTCAGCAC AACCACACAA
    TCAGGAGACC TGCCATCCAA GGAGAGAGAC CGGGGCCGAG CCAAAGACCG CAAGCACCAT
    CATCATCATC ATCACCACCA CGGCTCTTTA GATAAGGAGC ACTACGGTCA TGAGAGAGAC
    AGAGGAGAGT ACGGCCACAG ACAGTCCAGA GAGAGAGACC GCCGCTGGTC CCGCTCACCC
    AGTGAGGGCA GAGAGTGTCT GACTCACAGA CAGGGCAGTA GTTCAGTAAG CGGCAGTCCA
    GTCCCGTCCA CCTCCGGGAC CAGCACCCCC CGCCGTGGCC GCCGTCAGCT CCCCCAAACC
    CCTGCTACAC CCCGGCCACA TGTCACCTAC TCACCTGTGG TCCGTAAGCC CATCAGCAGT
    ACCCCCCCTC CTGGCCAGCA GAGCCGACTC CCTACCCCGA CCTCCCGGCG CTTCAGTCCG
    ACTGGCCCCG AGCCCCCGCT TCCCCCTCAT CACCACCCGT CACCACCCCA CCACGGCTCT
    CCCCGCTCCG GCCGACATGC ACACTGGGGC CCCGAGCCTG CAGAGAGCGT GGAGGGTGAC
    GGCTTCTATG ACGACCAAGA CTATGAGTTC AATCACCACG AGCCTCCATC CTACGAGCAA
    AGCCCTGTGC AGGGCAACCC TCACCCCCAT TCCCCTAGGA CTTCCCGCCA CAACACCCCT
    CCTCAGGGCC AAGCGCACCC ACGGCGCATG CCAAATGGCT ACCGTTCGTC TTCGCCCTCT
    CCACACCACC ACGCGCCCGC TCACCCTGGA ACTCATAAAC CTCCTCATCC CAGAGGGCCC
    AGAAAGGGCC TCCATGAACC CTACAGTGAG ACTGACGAGG ATGACTGGTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017210280.1
    CDS1466..8539
    Translation

    Target ORF information:

    RefSeq Version XM_017354791.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017354791.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGTGGCAGAA TCAGTTACAG GGATATGTAT GAGATGCTCA GGCACATGTC TCCTCCGTTA
    GGCCTGGGGA AGAAGTGCCC TGCACGGGTT GCCTACAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT
    GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC
    CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC
    ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT
    GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT
    GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT
    GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT
    CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA
    AGGCAGTCTC AGACAGTAGA GATGAGAGAG ATGGGGCGGG ACGGGTACTC GGACACAGAG
    CAATATCTGC CGATGGAAGG CCATGGCAGG GCTGCTTCCA TGCCTCGACT CCCAGCAGAC
    AACCAACAGC CTATCACAGA TAACAGTCCT ATGAAGCGCT CGGCTTCATC TTTGGGACAC
    GGGCGTGCAG GACGCTCCAT GCGAGGTGAG GATTATGCCA TGGAGAGGGT TATCCCAGAA
    GAGGGTCACA GGCATGGGCA TCGCCACCGA GACCGTAGTC ACCGGGCGTC AGAACGCTCC
    CTGAGCCGAT ACACTGATGC TGACACAGGT CTTGGAACTG ACCTCAGCAC AACCACACAA
    TCAGGAGACC TGCCATCCAA GGAGAGAGAC CGGGGCCGAG CCAAAGACCG CAAGCACCAT
    CATCATCATC ATCACCACCA CGGCTCTTTA GATAAGGAGC ACTACGGTCA TGAGAGAGAC
    AGAGGAGAGT ACGGCCACAG ACAGTCCAGA GAGAGAGACC GCCGCTGGTC CCGCTCACCC
    AGTGAGGGCA GAGAGTGTCT GACTCACAGA CAGGGCAGTA GTTCAGTAAG CGGCAGTCCA
    GTCCCGTCCA CCTCCGGGAC CAGCACCCCC CGCCGTGGCC GCCGTCAGCT CCCCCAAACC
    CCTGCTACAC CCCGGCCACA TGTCACCTAC TCACCTGTGG TCCGTAAGCC CATCAGCAGT
    ACCCCCCCTC CTGGCCAGCA GAGCCGACTC CCTACCCCGA CCTCCCGGCG CTTCAGTCCG
    ACTGGCCCCG AGCCCCCGCT TCCCCCTCAT CACCACCCGT CACCACCCCA CCACGGCTCT
    CCCCGCTCCG GCCGACATGC ACACTGGGGC CCCGAGCCTG CAGAGAGCGT GGAGGGTGAC
    GGCTTCTATG ACGACCAAGA CTATGAGTTC AATCACCACG AGCCTCCATC CTACGAGCAA
    AGCCCTGTGC AGGGCAACCC TCACCCCCAT TCCCCTAGGA CTTCCCGCCA CAACACCCCT
    CCTCAGGGCC AAGCGCACCC ACGGCGCATG CCAAATGGCT ACCGTTCGTC TTCGCCCTCT
    CCACACCACC ACGCGCCCGC TCACCCTGGA ACTCATAAAC CTCCTCATCC CAGAGGGCCC
    AGAAAGGGCC TCCATGAACC CTACAGTGAG ACTGACGAGG ATGACTGGTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa58248
    Clone ID Related Accession (Same CDS sequence) XM_017354792.1
    Accession Version XM_017354792.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7071bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA ACAACTTCAT AAATCTGAGT
    TTTCTAAGGC TCTTCAGAGC TGCTCGTCTC ATTAAGTTGC TCAGGCAGGG TGAAACCATA
    CGCATCCTGC TCTGGACTTT TGTCCAGTCT TTCAAGGCTC TGCCGTATGT GTGCCTACTC
    ATCGCCATGC TGTTCTTCAT CTACGCCATC ATTGGCATGC AGCTGTTTGG GAACATTAAG
    ATCGAGGAGA ACAGTGACAG TGCCATTACC CAGCACAACA ACTTCAGGAC TTTTTTCCAG
    GCCCTAATGC TGCTCTTTAG GAGTGCTACA GGTGAGGCAT GGCATGACAT CATGCTGTCA
    TGTCTGGGAA AGAAGCCGTG TGATATTCTC TCTGATAACC CGAAGCCAGA ATGCGGGAGC
    GAGTTTGCCT ACCTCTACTT TGTCTCCTTC ATCTTCCTCT GTTCATTTCT GATGCTGAAT
    CTGTTCGTGG CTGTGATCAT GGATAATTTT GAATACCTGA CGCGTGACTC CTCTATCCTG
    GGACCCCATC ACCTGGACGA GTATGTGAGG ATCTGGGCAG AATATGATCC TGCTGCTTGC
    GGGCGCATTC ATTATAAGGA TATGTACAGT TTATTGCGAG TTATCGACCC CCCTCTGGGC
    TTAGGCAAGA AATGTCCTCA TAGGGTGGCC TGCAAGAGAC TGTTGCGGAT GGATCTGCCG
    GTTGCCGATG ACAACACAGT GCACTTTAAT TCTACCCTTA TGGCCTTAAT CCGAACCGCT
    CTGGACATCA AGATCGCCAA GGGTGGGGAA GGAGGTGTAG ATAAGCACCA GATGGATGCT
    GAATTAAGGA AGGAGATGAT GGCGATATGG CCTAATCTCT CTCAGAAGAA CCTGGACCTG
    CTGGTGACTC CTCACAAGTC AGCGACAGAC CTGACAGTAG GAAAGATCTA CGCTGCCATG
    ATGATCATGG AGTATTATCG GCAAAGTAAA GCCAAACGGA CACAAGCTCT CCATGATGAG
    CAGAATCGAA CGCCATTAAT GTTTCAGCGT CTGGAGCCGC CATCTCCTAG CCAGGATGTG
    GGACCAGGAC TCACTGGCCT TCCTGACACA CAAATGCACC CTGCAAATCA TCTGCCTGTT
    GATGAAAGGA TCCCAGAGAG CCAATCATGG GTGACAGCGC GAGCACAGGA GATTTCTCAA
    AAAGCAGGCA ACTGGAGTCC AGAGGGCCAA CATCCAGATG ACACCACAGA CAACAGAAGG
    CAGTCTCAGA CAGTAGAGAT GAGAGAGATG GGGCGGGACG GGTACTCGGA CACAGAGCAA
    TATCTGCCGA TGGAAGGCCA TGGCAGGGCT GCTTCCATGC CTCGACTCCC AGCAGACAAC
    CAACAGCCTA TCACAGATAA CAGTCCTATG AAGCGCTCGG CTTCATCTTT GGGACACGGG
    CGTGCAGGAC GCTCCATGCG AGGTGAGGAT TATGCCATGG AGAGGGTTAT CCCAGAAGAG
    GGTCACAGGC ATGGGCATCG CCACCGAGAC CGTAGTCACC GGGCGTCAGA ACGCTCCCTG
    AGCCGATACA CTGATGCTGA CACAGGTCTT GGAACTGACC TCAGCACAAC CACACAATCA
    GGAGACCTGC CATCCAAGGA GAGAGACCGG GGCCGAGCCA AAGACCGCAA GCACCATCAT
    CATCATCATC ACCACCACGG CTCTTTAGAT AAGGAGCACT ACGGTCATGA GAGAGACAGA
    GGAGAGTACG GCCACAGACA GTCCAGAGAG AGAGACCGCC GCTGGTCCCG CTCACCCAGT
    GAGGGCAGAG AGTGTCTGAC TCACAGACAG GGCAGTAGTT CAGTAAGCGG CAGTCCAGTC
    CCGTCCACCT CCGGGACCAG CACCCCCCGC CGTGGCCGCC GTCAGCTCCC CCAAACCCCT
    GCTACACCCC GGCCACATGT CACCTACTCA CCTGTGGTCC GTAAGCCCAT CAGCAGTACC
    CCCCCTCCTG GCCAGCAGAG CCGACTCCCT ACCCCGACCT CCCGGCGCTT CAGTCCGACT
    GGCCCCGAGC CCCCGCTTCC CCCTCATCAC CACCCGTCAC CACCCCACCA CGGCTCTCCC
    CGCTCCGGCC GACATGCACA CTGGGGCCCC GAGCCTGCAG AGAGCGTGGA GGGTGACGGC
    TTCTATGACG ACCAAGACTA TGAGTTCAAT CACCACGAGC CTCCATCCTA CGAGCAAAGC
    CCTGTGCAGG GCAACCCTCA CCCCCATTCC CCTAGGACTT CCCGCCACAA CACCCCTCCT
    CAGGGCCAAG CGCACCCACG GCGCATGCCA AATGGCTACC GTTCGTCTTC GCCCTCTCCA
    CACCACCACG CGCCCGCTCA CCCTGGAACT CATAAACCTC CTCATCCCAG AGGGCCCAGA
    AAGGGCCTCC ATGAACCCTA CAGTGAGACT GACGAGGATG ACTGGTGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017210281.1
    CDS1466..8536
    Translation

    Target ORF information:

    RefSeq Version XM_017354792.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017354792.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA ACAACTTCAT AAATCTGAGT
    TTTCTAAGGC TCTTCAGAGC TGCTCGTCTC ATTAAGTTGC TCAGGCAGGG TGAAACCATA
    CGCATCCTGC TCTGGACTTT TGTCCAGTCT TTCAAGGCTC TGCCGTATGT GTGCCTACTC
    ATCGCCATGC TGTTCTTCAT CTACGCCATC ATTGGCATGC AGCTGTTTGG GAACATTAAG
    ATCGAGGAGA ACAGTGACAG TGCCATTACC CAGCACAACA ACTTCAGGAC TTTTTTCCAG
    GCCCTAATGC TGCTCTTTAG GAGTGCTACA GGTGAGGCAT GGCATGACAT CATGCTGTCA
    TGTCTGGGAA AGAAGCCGTG TGATATTCTC TCTGATAACC CGAAGCCAGA ATGCGGGAGC
    GAGTTTGCCT ACCTCTACTT TGTCTCCTTC ATCTTCCTCT GTTCATTTCT GATGCTGAAT
    CTGTTCGTGG CTGTGATCAT GGATAATTTT GAATACCTGA CGCGTGACTC CTCTATCCTG
    GGACCCCATC ACCTGGACGA GTATGTGAGG ATCTGGGCAG AATATGATCC TGCTGCTTGC
    GGGCGCATTC ATTATAAGGA TATGTACAGT TTATTGCGAG TTATCGACCC CCCTCTGGGC
    TTAGGCAAGA AATGTCCTCA TAGGGTGGCC TGCAAGAGAC TGTTGCGGAT GGATCTGCCG
    GTTGCCGATG ACAACACAGT GCACTTTAAT TCTACCCTTA TGGCCTTAAT CCGAACCGCT
    CTGGACATCA AGATCGCCAA GGGTGGGGAA GGAGGTGTAG ATAAGCACCA GATGGATGCT
    GAATTAAGGA AGGAGATGAT GGCGATATGG CCTAATCTCT CTCAGAAGAA CCTGGACCTG
    CTGGTGACTC CTCACAAGTC AGCGACAGAC CTGACAGTAG GAAAGATCTA CGCTGCCATG
    ATGATCATGG AGTATTATCG GCAAAGTAAA GCCAAACGGA CACAAGCTCT CCATGATGAG
    CAGAATCGAA CGCCATTAAT GTTTCAGCGT CTGGAGCCGC CATCTCCTAG CCAGGATGTG
    GGACCAGGAC TCACTGGCCT TCCTGACACA CAAATGCACC CTGCAAATCA TCTGCCTGTT
    GATGAAAGGA TCCCAGAGAG CCAATCATGG GTGACAGCGC GAGCACAGGA GATTTCTCAA
    AAAGCAGGCA ACTGGAGTCC AGAGGGCCAA CATCCAGATG ACACCACAGA CAACAGAAGG
    CAGTCTCAGA CAGTAGAGAT GAGAGAGATG GGGCGGGACG GGTACTCGGA CACAGAGCAA
    TATCTGCCGA TGGAAGGCCA TGGCAGGGCT GCTTCCATGC CTCGACTCCC AGCAGACAAC
    CAACAGCCTA TCACAGATAA CAGTCCTATG AAGCGCTCGG CTTCATCTTT GGGACACGGG
    CGTGCAGGAC GCTCCATGCG AGGTGAGGAT TATGCCATGG AGAGGGTTAT CCCAGAAGAG
    GGTCACAGGC ATGGGCATCG CCACCGAGAC CGTAGTCACC GGGCGTCAGA ACGCTCCCTG
    AGCCGATACA CTGATGCTGA CACAGGTCTT GGAACTGACC TCAGCACAAC CACACAATCA
    GGAGACCTGC CATCCAAGGA GAGAGACCGG GGCCGAGCCA AAGACCGCAA GCACCATCAT
    CATCATCATC ACCACCACGG CTCTTTAGAT AAGGAGCACT ACGGTCATGA GAGAGACAGA
    GGAGAGTACG GCCACAGACA GTCCAGAGAG AGAGACCGCC GCTGGTCCCG CTCACCCAGT
    GAGGGCAGAG AGTGTCTGAC TCACAGACAG GGCAGTAGTT CAGTAAGCGG CAGTCCAGTC
    CCGTCCACCT CCGGGACCAG CACCCCCCGC CGTGGCCGCC GTCAGCTCCC CCAAACCCCT
    GCTACACCCC GGCCACATGT CACCTACTCA CCTGTGGTCC GTAAGCCCAT CAGCAGTACC
    CCCCCTCCTG GCCAGCAGAG CCGACTCCCT ACCCCGACCT CCCGGCGCTT CAGTCCGACT
    GGCCCCGAGC CCCCGCTTCC CCCTCATCAC CACCCGTCAC CACCCCACCA CGGCTCTCCC
    CGCTCCGGCC GACATGCACA CTGGGGCCCC GAGCCTGCAG AGAGCGTGGA GGGTGACGGC
    TTCTATGACG ACCAAGACTA TGAGTTCAAT CACCACGAGC CTCCATCCTA CGAGCAAAGC
    CCTGTGCAGG GCAACCCTCA CCCCCATTCC CCTAGGACTT CCCGCCACAA CACCCCTCCT
    CAGGGCCAAG CGCACCCACG GCGCATGCCA AATGGCTACC GTTCGTCTTC GCCCTCTCCA
    CACCACCACG CGCCCGCTCA CCCTGGAACT CATAAACCTC CTCATCCCAG AGGGCCCAGA
    AAGGGCCTCC ATGAACCCTA CAGTGAGACT GACGAGGATG ACTGGTGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa58249
    Clone ID Related Accession (Same CDS sequence) XM_017354793.1
    Accession Version XM_017354793.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7071bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG
    GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT
    GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC
    CTGCTGGTGA CTCCTCACAA GTCGACAGAC CTGACAGTAG GAAAGATCTA CGCTGCCATG
    ATGATCATGG AGTATTATCG GCAAAGTAAA GCCAAACGGA CACAAGCTCT CCATGATGAG
    CAGAATCGAA CGCCATTAAT GTTTCAGCGT CTGGAGCCGC CATCTCCTAG CCAGGATGTG
    GGACCAGGAC TCACTGGCCT TCCTGACACA CAAATGCACC CTGCAAATCA TCTGCCTGTT
    GATGAAAGGA TCCCAGAGAG CCAATCATGG GTGACAGCGC GAGCACAGGA GATTTCTCAA
    AAAGCAGGCA ACTGGAGTCC AGAGGGCCAA CATCCAGATG ACACCACAGA CAACAGAAGG
    CAGTCTCAGA CAGTAGAGAT GAGAGAGATG GGGCGGGACG GGTACTCGGA CACAGAGCAA
    TATCTGCCGA TGGAAGGCCA TGGCAGGGCT GCTTCCATGC CTCGACTCCC AGCAGACAAC
    CAACAGCCTA TCACAGATAA CAGTCCTATG AAGCGCTCGG CTTCATCTTT GGGACACGGG
    CGTGCAGGAC GCTCCATGCG AGGTGAGGAT TATGCCATGG AGAGGGTTAT CCCAGAAGAG
    GGTCACAGGC ATGGGCATCG CCACCGAGAC CGTAGTCACC GGGCGTCAGA ACGCTCCCTG
    AGCCGATACA CTGATGCTGA CACAGGTCTT GGAACTGACC TCAGCACAAC CACACAATCA
    GGAGACCTGC CATCCAAGGA GAGAGACCGG GGCCGAGCCA AAGACCGCAA GCACCATCAT
    CATCATCATC ACCACCACGG CTCTTTAGAT AAGGAGCACT ACGGTCATGA GAGAGACAGA
    GGAGAGTACG GCCACAGACA GTCCAGAGAG AGAGACCGCC GCTGGTCCCG CTCACCCAGT
    GAGGGCAGAG AGTGTCTGAC TCACAGACAG GGCAGTAGTT CAGTAAGCGG CAGTCCAGTC
    CCGTCCACCT CCGGGACCAG CACCCCCCGC CGTGGCCGCC GTCAGCTCCC CCAAACCCCT
    GCTACACCCC GGCCACATGT CACCTACTCA CCTGTGGTCC GTAAGCCCAT CAGCAGTACC
    CCCCCTCCTG GCCAGCAGAG CCGACTCCCT ACCCCGACCT CCCGGCGCTT CAGTCCGACT
    GGCCCCGAGC CCCCGCTTCC CCCTCATCAC CACCCGTCAC CACCCCACCA CGGCTCTCCC
    CGCTCCGGCC GACATGCACA CTGGGGCCCC GAGCCTGCAG AGAGCGTGGA GGGTGACGGC
    TTCTATGACG ACCAAGACTA TGAGTTCAAT CACCACGAGC CTCCATCCTA CGAGCAAAGC
    CCTGTGCAGG GCAACCCTCA CCCCCATTCC CCTAGGACTT CCCGCCACAA CACCCCTCCT
    CAGGGCCAAG CGCACCCACG GCGCATGCCA AATGGCTACC GTTCGTCTTC GCCCTCTCCA
    CACCACCACG CGCCCGCTCA CCCTGGAACT CATAAACCTC CTCATCCCAG AGGGCCCAGA
    AAGGGCCTCC ATGAACCCTA CAGTGAGACT GACGAGGATG ACTGGTGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017210282.1
    CDS1467..8537
    Translation

    Target ORF information:

    RefSeq Version XM_017354793.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017354793.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG
    GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT
    GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC
    CTGCTGGTGA CTCCTCACAA GTCGACAGAC CTGACAGTAG GAAAGATCTA CGCTGCCATG
    ATGATCATGG AGTATTATCG GCAAAGTAAA GCCAAACGGA CACAAGCTCT CCATGATGAG
    CAGAATCGAA CGCCATTAAT GTTTCAGCGT CTGGAGCCGC CATCTCCTAG CCAGGATGTG
    GGACCAGGAC TCACTGGCCT TCCTGACACA CAAATGCACC CTGCAAATCA TCTGCCTGTT
    GATGAAAGGA TCCCAGAGAG CCAATCATGG GTGACAGCGC GAGCACAGGA GATTTCTCAA
    AAAGCAGGCA ACTGGAGTCC AGAGGGCCAA CATCCAGATG ACACCACAGA CAACAGAAGG
    CAGTCTCAGA CAGTAGAGAT GAGAGAGATG GGGCGGGACG GGTACTCGGA CACAGAGCAA
    TATCTGCCGA TGGAAGGCCA TGGCAGGGCT GCTTCCATGC CTCGACTCCC AGCAGACAAC
    CAACAGCCTA TCACAGATAA CAGTCCTATG AAGCGCTCGG CTTCATCTTT GGGACACGGG
    CGTGCAGGAC GCTCCATGCG AGGTGAGGAT TATGCCATGG AGAGGGTTAT CCCAGAAGAG
    GGTCACAGGC ATGGGCATCG CCACCGAGAC CGTAGTCACC GGGCGTCAGA ACGCTCCCTG
    AGCCGATACA CTGATGCTGA CACAGGTCTT GGAACTGACC TCAGCACAAC CACACAATCA
    GGAGACCTGC CATCCAAGGA GAGAGACCGG GGCCGAGCCA AAGACCGCAA GCACCATCAT
    CATCATCATC ACCACCACGG CTCTTTAGAT AAGGAGCACT ACGGTCATGA GAGAGACAGA
    GGAGAGTACG GCCACAGACA GTCCAGAGAG AGAGACCGCC GCTGGTCCCG CTCACCCAGT
    GAGGGCAGAG AGTGTCTGAC TCACAGACAG GGCAGTAGTT CAGTAAGCGG CAGTCCAGTC
    CCGTCCACCT CCGGGACCAG CACCCCCCGC CGTGGCCGCC GTCAGCTCCC CCAAACCCCT
    GCTACACCCC GGCCACATGT CACCTACTCA CCTGTGGTCC GTAAGCCCAT CAGCAGTACC
    CCCCCTCCTG GCCAGCAGAG CCGACTCCCT ACCCCGACCT CCCGGCGCTT CAGTCCGACT
    GGCCCCGAGC CCCCGCTTCC CCCTCATCAC CACCCGTCAC CACCCCACCA CGGCTCTCCC
    CGCTCCGGCC GACATGCACA CTGGGGCCCC GAGCCTGCAG AGAGCGTGGA GGGTGACGGC
    TTCTATGACG ACCAAGACTA TGAGTTCAAT CACCACGAGC CTCCATCCTA CGAGCAAAGC
    CCTGTGCAGG GCAACCCTCA CCCCCATTCC CCTAGGACTT CCCGCCACAA CACCCCTCCT
    CAGGGCCAAG CGCACCCACG GCGCATGCCA AATGGCTACC GTTCGTCTTC GCCCTCTCCA
    CACCACCACG CGCCCGCTCA CCCTGGAACT CATAAACCTC CTCATCCCAG AGGGCCCAGA
    AAGGGCCTCC ATGAACCCTA CAGTGAGACT GACGAGGATG ACTGGTGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa58250
    Clone ID Related Accession (Same CDS sequence) XM_017354794.2 , XM_017354794.1
    Accession Version XM_017354794.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7071bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017354794.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG
    GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT
    GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC
    CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC
    ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT
    GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT
    GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT
    GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT
    CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA
    AGGCAGTCTC AGACAGTAGA GATGAGAGAG ATGGGGCGGG ACGGGTACTC GGACACAGAG
    CAATATCTGC CGATGGAAGG CCATGGCAGG GCTGCTTCCA TGCCTCGACT CCCAGCAGAC
    AACCAACCTA TCACAGATAA CAGTCCTATG AAGCGCTCGG CTTCATCTTT GGGACACGGG
    CGTGCAGGAC GCTCCATGCG AGGTGAGGAT TATGCCATGG AGAGGGTTAT CCCAGAAGAG
    GGTCACAGGC ATGGGCATCG CCACCGAGAC CGTAGTCACC GGGCGTCAGA ACGCTCCCTG
    AGCCGATACA CTGATGCTGA CACAGGTCTT GGAACTGACC TCAGCACAAC CACACAATCA
    GGAGACCTGC CATCCAAGGA GAGAGACCGG GGCCGAGCCA AAGACCGCAA GCACCATCAT
    CATCATCATC ACCACCACGG CTCTTTAGAT AAGGAGCACT ACGGTCATGA GAGAGACAGA
    GGAGAGTACG GCCACAGACA GTCCAGAGAG AGAGACCGCC GCTGGTCCCG CTCACCCAGT
    GAGGGCAGAG AGTGTCTGAC TCACAGACAG GGCAGTAGTT CAGTAAGCGG CAGTCCAGTC
    CCGTCCACCT CCGGGACCAG CACCCCCCGC CGTGGCCGCC GTCAGCTCCC CCAAACCCCT
    GCTACACCCC GGCCACATGT CACCTACTCA CCTGTGGTCC GTAAGCCCAT CAGCAGTACC
    CCCCCTCCTG GCCAGCAGAG CCGACTCCCT ACCCCGACCT CCCGGCGCTT CAGTCCGACT
    GGCCCCGAGC CCCCGCTTCC CCCTCATCAC CACCCGTCAC CACCCCACCA CGGCTCTCCC
    CGCTCCGGCC GACATGCACA CTGGGGCCCC GAGCCTGCAG AGAGCGTGGA GGGTGACGGC
    TTCTATGACG ACCAAGACTA TGAGTTCAAT CACCACGAGC CTCCATCCTA CGAGCAAAGC
    CCTGTGCAGG GCAACCCTCA CCCCCATTCC CCTAGGACTT CCCGCCACAA CACCCCTCCT
    CAGGGCCAAG CGCACCCACG GCGCATGCCA AATGGCTACC GTTCGTCTTC GCCCTCTCCA
    CACCACCACG CGCCCGCTCA CCCTGGAACT CATAAACCTC CTCATCCCAG AGGGCCCAGA
    AAGGGCCTCC ATGAACCCTA CAGTGAGACT GACGAGGATG ACTGGTGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017210283.1
    CDS1506..8576
    Translation

    Target ORF information:

    RefSeq Version XM_017354794.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017354794.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG
    GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT
    GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC
    CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC
    ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT
    GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT
    GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT
    GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT
    CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA
    AGGCAGTCTC AGACAGTAGA GATGAGAGAG ATGGGGCGGG ACGGGTACTC GGACACAGAG
    CAATATCTGC CGATGGAAGG CCATGGCAGG GCTGCTTCCA TGCCTCGACT CCCAGCAGAC
    AACCAACCTA TCACAGATAA CAGTCCTATG AAGCGCTCGG CTTCATCTTT GGGACACGGG
    CGTGCAGGAC GCTCCATGCG AGGTGAGGAT TATGCCATGG AGAGGGTTAT CCCAGAAGAG
    GGTCACAGGC ATGGGCATCG CCACCGAGAC CGTAGTCACC GGGCGTCAGA ACGCTCCCTG
    AGCCGATACA CTGATGCTGA CACAGGTCTT GGAACTGACC TCAGCACAAC CACACAATCA
    GGAGACCTGC CATCCAAGGA GAGAGACCGG GGCCGAGCCA AAGACCGCAA GCACCATCAT
    CATCATCATC ACCACCACGG CTCTTTAGAT AAGGAGCACT ACGGTCATGA GAGAGACAGA
    GGAGAGTACG GCCACAGACA GTCCAGAGAG AGAGACCGCC GCTGGTCCCG CTCACCCAGT
    GAGGGCAGAG AGTGTCTGAC TCACAGACAG GGCAGTAGTT CAGTAAGCGG CAGTCCAGTC
    CCGTCCACCT CCGGGACCAG CACCCCCCGC CGTGGCCGCC GTCAGCTCCC CCAAACCCCT
    GCTACACCCC GGCCACATGT CACCTACTCA CCTGTGGTCC GTAAGCCCAT CAGCAGTACC
    CCCCCTCCTG GCCAGCAGAG CCGACTCCCT ACCCCGACCT CCCGGCGCTT CAGTCCGACT
    GGCCCCGAGC CCCCGCTTCC CCCTCATCAC CACCCGTCAC CACCCCACCA CGGCTCTCCC
    CGCTCCGGCC GACATGCACA CTGGGGCCCC GAGCCTGCAG AGAGCGTGGA GGGTGACGGC
    TTCTATGACG ACCAAGACTA TGAGTTCAAT CACCACGAGC CTCCATCCTA CGAGCAAAGC
    CCTGTGCAGG GCAACCCTCA CCCCCATTCC CCTAGGACTT CCCGCCACAA CACCCCTCCT
    CAGGGCCAAG CGCACCCACG GCGCATGCCA AATGGCTACC GTTCGTCTTC GCCCTCTCCA
    CACCACCACG CGCCCGCTCA CCCTGGAACT CATAAACCTC CTCATCCCAG AGGGCCCAGA
    AAGGGCCTCC ATGAACCCTA CAGTGAGACT GACGAGGATG ACTGGTGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa58250
    Clone ID Related Accession (Same CDS sequence) XM_017354794.2 , XM_017354794.1
    Accession Version XM_017354794.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7071bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG
    GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT
    GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC
    CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC
    ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT
    GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT
    GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT
    GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT
    CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA
    AGGCAGTCTC AGACAGTAGA GATGAGAGAG ATGGGGCGGG ACGGGTACTC GGACACAGAG
    CAATATCTGC CGATGGAAGG CCATGGCAGG GCTGCTTCCA TGCCTCGACT CCCAGCAGAC
    AACCAACCTA TCACAGATAA CAGTCCTATG AAGCGCTCGG CTTCATCTTT GGGACACGGG
    CGTGCAGGAC GCTCCATGCG AGGTGAGGAT TATGCCATGG AGAGGGTTAT CCCAGAAGAG
    GGTCACAGGC ATGGGCATCG CCACCGAGAC CGTAGTCACC GGGCGTCAGA ACGCTCCCTG
    AGCCGATACA CTGATGCTGA CACAGGTCTT GGAACTGACC TCAGCACAAC CACACAATCA
    GGAGACCTGC CATCCAAGGA GAGAGACCGG GGCCGAGCCA AAGACCGCAA GCACCATCAT
    CATCATCATC ACCACCACGG CTCTTTAGAT AAGGAGCACT ACGGTCATGA GAGAGACAGA
    GGAGAGTACG GCCACAGACA GTCCAGAGAG AGAGACCGCC GCTGGTCCCG CTCACCCAGT
    GAGGGCAGAG AGTGTCTGAC TCACAGACAG GGCAGTAGTT CAGTAAGCGG CAGTCCAGTC
    CCGTCCACCT CCGGGACCAG CACCCCCCGC CGTGGCCGCC GTCAGCTCCC CCAAACCCCT
    GCTACACCCC GGCCACATGT CACCTACTCA CCTGTGGTCC GTAAGCCCAT CAGCAGTACC
    CCCCCTCCTG GCCAGCAGAG CCGACTCCCT ACCCCGACCT CCCGGCGCTT CAGTCCGACT
    GGCCCCGAGC CCCCGCTTCC CCCTCATCAC CACCCGTCAC CACCCCACCA CGGCTCTCCC
    CGCTCCGGCC GACATGCACA CTGGGGCCCC GAGCCTGCAG AGAGCGTGGA GGGTGACGGC
    TTCTATGACG ACCAAGACTA TGAGTTCAAT CACCACGAGC CTCCATCCTA CGAGCAAAGC
    CCTGTGCAGG GCAACCCTCA CCCCCATTCC CCTAGGACTT CCCGCCACAA CACCCCTCCT
    CAGGGCCAAG CGCACCCACG GCGCATGCCA AATGGCTACC GTTCGTCTTC GCCCTCTCCA
    CACCACCACG CGCCCGCTCA CCCTGGAACT CATAAACCTC CTCATCCCAG AGGGCCCAGA
    AAGGGCCTCC ATGAACCCTA CAGTGAGACT GACGAGGATG ACTGGTGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017210283.1
    CDS1467..8537
    Translation

    Target ORF information:

    RefSeq Version XM_017354794.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017354794.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG
    GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT
    GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC
    CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC
    ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT
    GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT
    GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT
    GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT
    CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA
    AGGCAGTCTC AGACAGTAGA GATGAGAGAG ATGGGGCGGG ACGGGTACTC GGACACAGAG
    CAATATCTGC CGATGGAAGG CCATGGCAGG GCTGCTTCCA TGCCTCGACT CCCAGCAGAC
    AACCAACCTA TCACAGATAA CAGTCCTATG AAGCGCTCGG CTTCATCTTT GGGACACGGG
    CGTGCAGGAC GCTCCATGCG AGGTGAGGAT TATGCCATGG AGAGGGTTAT CCCAGAAGAG
    GGTCACAGGC ATGGGCATCG CCACCGAGAC CGTAGTCACC GGGCGTCAGA ACGCTCCCTG
    AGCCGATACA CTGATGCTGA CACAGGTCTT GGAACTGACC TCAGCACAAC CACACAATCA
    GGAGACCTGC CATCCAAGGA GAGAGACCGG GGCCGAGCCA AAGACCGCAA GCACCATCAT
    CATCATCATC ACCACCACGG CTCTTTAGAT AAGGAGCACT ACGGTCATGA GAGAGACAGA
    GGAGAGTACG GCCACAGACA GTCCAGAGAG AGAGACCGCC GCTGGTCCCG CTCACCCAGT
    GAGGGCAGAG AGTGTCTGAC TCACAGACAG GGCAGTAGTT CAGTAAGCGG CAGTCCAGTC
    CCGTCCACCT CCGGGACCAG CACCCCCCGC CGTGGCCGCC GTCAGCTCCC CCAAACCCCT
    GCTACACCCC GGCCACATGT CACCTACTCA CCTGTGGTCC GTAAGCCCAT CAGCAGTACC
    CCCCCTCCTG GCCAGCAGAG CCGACTCCCT ACCCCGACCT CCCGGCGCTT CAGTCCGACT
    GGCCCCGAGC CCCCGCTTCC CCCTCATCAC CACCCGTCAC CACCCCACCA CGGCTCTCCC
    CGCTCCGGCC GACATGCACA CTGGGGCCCC GAGCCTGCAG AGAGCGTGGA GGGTGACGGC
    TTCTATGACG ACCAAGACTA TGAGTTCAAT CACCACGAGC CTCCATCCTA CGAGCAAAGC
    CCTGTGCAGG GCAACCCTCA CCCCCATTCC CCTAGGACTT CCCGCCACAA CACCCCTCCT
    CAGGGCCAAG CGCACCCACG GCGCATGCCA AATGGCTACC GTTCGTCTTC GCCCTCTCCA
    CACCACCACG CGCCCGCTCA CCCTGGAACT CATAAACCTC CTCATCCCAG AGGGCCCAGA
    AAGGGCCTCC ATGAACCCTA CAGTGAGACT GACGAGGATG ACTGGTGCTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa58251
    Clone ID Related Accession (Same CDS sequence) XM_017354795.1
    Accession Version XM_017354795.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7065bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG
    GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGAGGT GTAGATAAGC ACCAGATGGA TGCTGAATTA
    AGGAAGGAGA TGATGGCGAT ATGGCCTAAT CTCTCTCAGA AGAACCTGGA CCTGCTGGTG
    ACTCCTCACA AGTCAGCGAC AGACCTGACA GTAGGAAAGA TCTACGCTGC CATGATGATC
    ATGGAGTATT ATCGGCAAAG TAAAGCCAAA CGGACACAAG CTCTCCATGA TGAGCAGAAT
    CGAACGCCAT TAATGTTTCA GCGTCTGGAG CCGCCATCTC CTAGCCAGGA TGTGGGACCA
    GGACTCACTG GCCTTCCTGA CACACAAATG CACCCTGCAA ATCATCTGCC TGTTGATGAA
    AGGATCCCAG AGAGCCAATC ATGGGTGACA GCGCGAGCAC AGGAGATTTC TCAAAAAGCA
    GGCAACTGGA GTCCAGAGGG CCAACATCCA GATGACACCA CAGACAACAG AAGGCAGTCT
    CAGACAGTAG AGATGAGAGA GATGGGGCGG GACGGGTACT CGGACACAGA GCAATATCTG
    CCGATGGAAG GCCATGGCAG GGCTGCTTCC ATGCCTCGAC TCCCAGCAGA CAACCAACAG
    CCTATCACAG ATAACAGTCC TATGAAGCGC TCGGCTTCAT CTTTGGGACA CGGGCGTGCA
    GGACGCTCCA TGCGAGGTGA GGATTATGCC ATGGAGAGGG TTATCCCAGA AGAGGGTCAC
    AGGCATGGGC ATCGCCACCG AGACCGTAGT CACCGGGCGT CAGAACGCTC CCTGAGCCGA
    TACACTGATG CTGACACAGG TCTTGGAACT GACCTCAGCA CAACCACACA ATCAGGAGAC
    CTGCCATCCA AGGAGAGAGA CCGGGGCCGA GCCAAAGACC GCAAGCACCA TCATCATCAT
    CATCACCACC ACGGCTCTTT AGATAAGGAG CACTACGGTC ATGAGAGAGA CAGAGGAGAG
    TACGGCCACA GACAGTCCAG AGAGAGAGAC CGCCGCTGGT CCCGCTCACC CAGTGAGGGC
    AGAGAGTGTC TGACTCACAG ACAGGGCAGT AGTTCAGTAA GCGGCAGTCC AGTCCCGTCC
    ACCTCCGGGA CCAGCACCCC CCGCCGTGGC CGCCGTCAGC TCCCCCAAAC CCCTGCTACA
    CCCCGGCCAC ATGTCACCTA CTCACCTGTG GTCCGTAAGC CCATCAGCAG TACCCCCCCT
    CCTGGCCAGC AGAGCCGACT CCCTACCCCG ACCTCCCGGC GCTTCAGTCC GACTGGCCCC
    GAGCCCCCGC TTCCCCCTCA TCACCACCCG TCACCACCCC ACCACGGCTC TCCCCGCTCC
    GGCCGACATG CACACTGGGG CCCCGAGCCT GCAGAGAGCG TGGAGGGTGA CGGCTTCTAT
    GACGACCAAG ACTATGAGTT CAATCACCAC GAGCCTCCAT CCTACGAGCA AAGCCCTGTG
    CAGGGCAACC CTCACCCCCA TTCCCCTAGG ACTTCCCGCC ACAACACCCC TCCTCAGGGC
    CAAGCGCACC CACGGCGCAT GCCAAATGGC TACCGTTCGT CTTCGCCCTC TCCACACCAC
    CACGCGCCCG CTCACCCTGG AACTCATAAA CCTCCTCATC CCAGAGGGCC CAGAAAGGGC
    CTCCATGAAC CCTACAGTGA GACTGACGAG GATGACTGGT GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017210284.1
    CDS1467..8531
    Translation

    Target ORF information:

    RefSeq Version XM_017354795.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017354795.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG
    GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGAGGT GTAGATAAGC ACCAGATGGA TGCTGAATTA
    AGGAAGGAGA TGATGGCGAT ATGGCCTAAT CTCTCTCAGA AGAACCTGGA CCTGCTGGTG
    ACTCCTCACA AGTCAGCGAC AGACCTGACA GTAGGAAAGA TCTACGCTGC CATGATGATC
    ATGGAGTATT ATCGGCAAAG TAAAGCCAAA CGGACACAAG CTCTCCATGA TGAGCAGAAT
    CGAACGCCAT TAATGTTTCA GCGTCTGGAG CCGCCATCTC CTAGCCAGGA TGTGGGACCA
    GGACTCACTG GCCTTCCTGA CACACAAATG CACCCTGCAA ATCATCTGCC TGTTGATGAA
    AGGATCCCAG AGAGCCAATC ATGGGTGACA GCGCGAGCAC AGGAGATTTC TCAAAAAGCA
    GGCAACTGGA GTCCAGAGGG CCAACATCCA GATGACACCA CAGACAACAG AAGGCAGTCT
    CAGACAGTAG AGATGAGAGA GATGGGGCGG GACGGGTACT CGGACACAGA GCAATATCTG
    CCGATGGAAG GCCATGGCAG GGCTGCTTCC ATGCCTCGAC TCCCAGCAGA CAACCAACAG
    CCTATCACAG ATAACAGTCC TATGAAGCGC TCGGCTTCAT CTTTGGGACA CGGGCGTGCA
    GGACGCTCCA TGCGAGGTGA GGATTATGCC ATGGAGAGGG TTATCCCAGA AGAGGGTCAC
    AGGCATGGGC ATCGCCACCG AGACCGTAGT CACCGGGCGT CAGAACGCTC CCTGAGCCGA
    TACACTGATG CTGACACAGG TCTTGGAACT GACCTCAGCA CAACCACACA ATCAGGAGAC
    CTGCCATCCA AGGAGAGAGA CCGGGGCCGA GCCAAAGACC GCAAGCACCA TCATCATCAT
    CATCACCACC ACGGCTCTTT AGATAAGGAG CACTACGGTC ATGAGAGAGA CAGAGGAGAG
    TACGGCCACA GACAGTCCAG AGAGAGAGAC CGCCGCTGGT CCCGCTCACC CAGTGAGGGC
    AGAGAGTGTC TGACTCACAG ACAGGGCAGT AGTTCAGTAA GCGGCAGTCC AGTCCCGTCC
    ACCTCCGGGA CCAGCACCCC CCGCCGTGGC CGCCGTCAGC TCCCCCAAAC CCCTGCTACA
    CCCCGGCCAC ATGTCACCTA CTCACCTGTG GTCCGTAAGC CCATCAGCAG TACCCCCCCT
    CCTGGCCAGC AGAGCCGACT CCCTACCCCG ACCTCCCGGC GCTTCAGTCC GACTGGCCCC
    GAGCCCCCGC TTCCCCCTCA TCACCACCCG TCACCACCCC ACCACGGCTC TCCCCGCTCC
    GGCCGACATG CACACTGGGG CCCCGAGCCT GCAGAGAGCG TGGAGGGTGA CGGCTTCTAT
    GACGACCAAG ACTATGAGTT CAATCACCAC GAGCCTCCAT CCTACGAGCA AAGCCCTGTG
    CAGGGCAACC CTCACCCCCA TTCCCCTAGG ACTTCCCGCC ACAACACCCC TCCTCAGGGC
    CAAGCGCACC CACGGCGCAT GCCAAATGGC TACCGTTCGT CTTCGCCCTC TCCACACCAC
    CACGCGCCCG CTCACCCTGG AACTCATAAA CCTCCTCATC CCAGAGGGCC CAGAAAGGGC
    CTCCATGAAC CCTACAGTGA GACTGACGAG GATGACTGGT GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa58252
    Clone ID Related Accession (Same CDS sequence) XM_017354796.1
    Accession Version XM_017354796.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7065bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGTGGCAGAA TCAGTTACAG GGATATGTAT GAGATGCTCA GGCACATGTC TCCTCCGTTA
    GGCCTGGGGA AGAAGTGCCC TGCACGGGTT GCCTACAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGAGGT GTAGATAAGC ACCAGATGGA TGCTGAATTA
    AGGAAGGAGA TGATGGCGAT ATGGCCTAAT CTCTCTCAGA AGAACCTGGA CCTGCTGGTG
    ACTCCTCACA AGTCAGCGAC AGACCTGACA GTAGGAAAGA TCTACGCTGC CATGATGATC
    ATGGAGTATT ATCGGCAAAG TAAAGCCAAA CGGACACAAG CTCTCCATGA TGAGCAGAAT
    CGAACGCCAT TAATGTTTCA GCGTCTGGAG CCGCCATCTC CTAGCCAGGA TGTGGGACCA
    GGACTCACTG GCCTTCCTGA CACACAAATG CACCCTGCAA ATCATCTGCC TGTTGATGAA
    AGGATCCCAG AGAGCCAATC ATGGGTGACA GCGCGAGCAC AGGAGATTTC TCAAAAAGCA
    GGCAACTGGA GTCCAGAGGG CCAACATCCA GATGACACCA CAGACAACAG AAGGCAGTCT
    CAGACAGTAG AGATGAGAGA GATGGGGCGG GACGGGTACT CGGACACAGA GCAATATCTG
    CCGATGGAAG GCCATGGCAG GGCTGCTTCC ATGCCTCGAC TCCCAGCAGA CAACCAACAG
    CCTATCACAG ATAACAGTCC TATGAAGCGC TCGGCTTCAT CTTTGGGACA CGGGCGTGCA
    GGACGCTCCA TGCGAGGTGA GGATTATGCC ATGGAGAGGG TTATCCCAGA AGAGGGTCAC
    AGGCATGGGC ATCGCCACCG AGACCGTAGT CACCGGGCGT CAGAACGCTC CCTGAGCCGA
    TACACTGATG CTGACACAGG TCTTGGAACT GACCTCAGCA CAACCACACA ATCAGGAGAC
    CTGCCATCCA AGGAGAGAGA CCGGGGCCGA GCCAAAGACC GCAAGCACCA TCATCATCAT
    CATCACCACC ACGGCTCTTT AGATAAGGAG CACTACGGTC ATGAGAGAGA CAGAGGAGAG
    TACGGCCACA GACAGTCCAG AGAGAGAGAC CGCCGCTGGT CCCGCTCACC CAGTGAGGGC
    AGAGAGTGTC TGACTCACAG ACAGGGCAGT AGTTCAGTAA GCGGCAGTCC AGTCCCGTCC
    ACCTCCGGGA CCAGCACCCC CCGCCGTGGC CGCCGTCAGC TCCCCCAAAC CCCTGCTACA
    CCCCGGCCAC ATGTCACCTA CTCACCTGTG GTCCGTAAGC CCATCAGCAG TACCCCCCCT
    CCTGGCCAGC AGAGCCGACT CCCTACCCCG ACCTCCCGGC GCTTCAGTCC GACTGGCCCC
    GAGCCCCCGC TTCCCCCTCA TCACCACCCG TCACCACCCC ACCACGGCTC TCCCCGCTCC
    GGCCGACATG CACACTGGGG CCCCGAGCCT GCAGAGAGCG TGGAGGGTGA CGGCTTCTAT
    GACGACCAAG ACTATGAGTT CAATCACCAC GAGCCTCCAT CCTACGAGCA AAGCCCTGTG
    CAGGGCAACC CTCACCCCCA TTCCCCTAGG ACTTCCCGCC ACAACACCCC TCCTCAGGGC
    CAAGCGCACC CACGGCGCAT GCCAAATGGC TACCGTTCGT CTTCGCCCTC TCCACACCAC
    CACGCGCCCG CTCACCCTGG AACTCATAAA CCTCCTCATC CCAGAGGGCC CAGAAAGGGC
    CTCCATGAAC CCTACAGTGA GACTGACGAG GATGACTGGT GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017210285.1
    CDS1467..8531
    Translation

    Target ORF information:

    RefSeq Version XM_017354796.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017354796.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGTGGCAGAA TCAGTTACAG GGATATGTAT GAGATGCTCA GGCACATGTC TCCTCCGTTA
    GGCCTGGGGA AGAAGTGCCC TGCACGGGTT GCCTACAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGAGGT GTAGATAAGC ACCAGATGGA TGCTGAATTA
    AGGAAGGAGA TGATGGCGAT ATGGCCTAAT CTCTCTCAGA AGAACCTGGA CCTGCTGGTG
    ACTCCTCACA AGTCAGCGAC AGACCTGACA GTAGGAAAGA TCTACGCTGC CATGATGATC
    ATGGAGTATT ATCGGCAAAG TAAAGCCAAA CGGACACAAG CTCTCCATGA TGAGCAGAAT
    CGAACGCCAT TAATGTTTCA GCGTCTGGAG CCGCCATCTC CTAGCCAGGA TGTGGGACCA
    GGACTCACTG GCCTTCCTGA CACACAAATG CACCCTGCAA ATCATCTGCC TGTTGATGAA
    AGGATCCCAG AGAGCCAATC ATGGGTGACA GCGCGAGCAC AGGAGATTTC TCAAAAAGCA
    GGCAACTGGA GTCCAGAGGG CCAACATCCA GATGACACCA CAGACAACAG AAGGCAGTCT
    CAGACAGTAG AGATGAGAGA GATGGGGCGG GACGGGTACT CGGACACAGA GCAATATCTG
    CCGATGGAAG GCCATGGCAG GGCTGCTTCC ATGCCTCGAC TCCCAGCAGA CAACCAACAG
    CCTATCACAG ATAACAGTCC TATGAAGCGC TCGGCTTCAT CTTTGGGACA CGGGCGTGCA
    GGACGCTCCA TGCGAGGTGA GGATTATGCC ATGGAGAGGG TTATCCCAGA AGAGGGTCAC
    AGGCATGGGC ATCGCCACCG AGACCGTAGT CACCGGGCGT CAGAACGCTC CCTGAGCCGA
    TACACTGATG CTGACACAGG TCTTGGAACT GACCTCAGCA CAACCACACA ATCAGGAGAC
    CTGCCATCCA AGGAGAGAGA CCGGGGCCGA GCCAAAGACC GCAAGCACCA TCATCATCAT
    CATCACCACC ACGGCTCTTT AGATAAGGAG CACTACGGTC ATGAGAGAGA CAGAGGAGAG
    TACGGCCACA GACAGTCCAG AGAGAGAGAC CGCCGCTGGT CCCGCTCACC CAGTGAGGGC
    AGAGAGTGTC TGACTCACAG ACAGGGCAGT AGTTCAGTAA GCGGCAGTCC AGTCCCGTCC
    ACCTCCGGGA CCAGCACCCC CCGCCGTGGC CGCCGTCAGC TCCCCCAAAC CCCTGCTACA
    CCCCGGCCAC ATGTCACCTA CTCACCTGTG GTCCGTAAGC CCATCAGCAG TACCCCCCCT
    CCTGGCCAGC AGAGCCGACT CCCTACCCCG ACCTCCCGGC GCTTCAGTCC GACTGGCCCC
    GAGCCCCCGC TTCCCCCTCA TCACCACCCG TCACCACCCC ACCACGGCTC TCCCCGCTCC
    GGCCGACATG CACACTGGGG CCCCGAGCCT GCAGAGAGCG TGGAGGGTGA CGGCTTCTAT
    GACGACCAAG ACTATGAGTT CAATCACCAC GAGCCTCCAT CCTACGAGCA AAGCCCTGTG
    CAGGGCAACC CTCACCCCCA TTCCCCTAGG ACTTCCCGCC ACAACACCCC TCCTCAGGGC
    CAAGCGCACC CACGGCGCAT GCCAAATGGC TACCGTTCGT CTTCGCCCTC TCCACACCAC
    CACGCGCCCG CTCACCCTGG AACTCATAAA CCTCCTCATC CCAGAGGGCC CAGAAAGGGC
    CTCCATGAAC CCTACAGTGA GACTGACGAG GATGACTGGT GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa58253
    Clone ID Related Accession (Same CDS sequence) XM_017354797.1
    Accession Version XM_017354797.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7062bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACAGGAAGC AGCAAAGGGA AAGACATCAG TACTATCAAG
    TCTCTACGTG TGCTTCGAGT ACTGCGGCCG CTGAAAACCA TCAAACGTCT GCCAAAACTG
    AAGGCTGTCT TTGACTGTGT GGTGAACTCT CTGAAGAATG TGTTGAACAT CCTCATCGTC
    TACATGCTCT TCATGTTCAT CTTCGCTGTG GTGGCTGTGC AGCTCTTTAA GGGACGTTTC
    TTTTACTGCA CTGATGAATC CAAAGAGTTT GAGCGCGACT GCAGGGGCGA GTATCTGGTC
    TATGAAAGAG ATAACGAAGT GAGGTCACAG AAGCGGGAAT GGAAGAAATA CGATTTCCAC
    TACGACAACG TGCTCTGGGC CCTTCTCACC CTCTTCACCG TTTCTACGGG AGAGGGGTGG
    CCGCAAGTGC TAAAACATTC AGTAGATGCG ACCTACGAGA ATCAGGGCCC GAGCCCAGGT
    TACCGGATGG AAATGTCCAT CTTTTACGTG GTGTACTTCG TGGTCTTCCC CTTCTTCTTC
    GTCAACATCT TCGTGGCTCT TATCATCATT ACTTTCCAGG AGCAAGGAGA CAAAATGATG
    GAGGACTATA GCCTGGAAAA GAATGAGAGA GCCTGCATAG ACTTTGCCAT CAACGCTAAG
    CCGCTCACAC GGCACATGCC CCAGAACAAG CAGACCTTTC AGTACCGCAT GTGGGAGTTT
    GTGGTGTCTC CTCCATTTGA ATACACCATC ATGGCCTTGA TCGCGCTCAA CACTATTGTC
    CTTATGATGA AGTATGATGG AGCATCGCTT ACTTATGAAG ATGTGCTAAA ATACCTGAAC
    ATAGTGTTCA CCTCTCTATT CTCCATGGAG TGCATATTGA AGATCATAGC CTTCGGAGCA
    TTGAACTACT TCAAAGATGC CTGGAACATC TTCGACTGTG TGACTGTACT TGGCAGCATC
    ACTGATATTT TAGTCACAGA GCTGGGGATG AACAACTTCA TAAATCTGAG TTTTCTAAGG
    CTCTTCAGAG CTGCTCGTCT CATTAAGTTG CTCAGGCAGG GTGAAACCAT ACGCATCCTG
    CTCTGGACTT TTGTCCAGTC TTTCAAGGCT CTGCCGTATG TGTGCCTACT CATCGCCATG
    CTGTTCTTCA TCTACGCCAT CATTGGCATG CAGCTGTTTG GGAACATTAA GATCGAGGAG
    AACAGTGACA GTGCCATTAC CCAGCACAAC AACTTCAGGA CTTTTTTCCA GGCCCTAATG
    CTGCTCTTTA GGAGTGCTAC AGGTGAGGCA TGGCATGACA TCATGCTGTC ATGTCTGGGA
    AAGAAGCCGT GTGATATTCT CTCTGATAAC CCGAAGCCAG AATGCGGGAG CGAGTTTGCC
    TACCTCTACT TTGTCTCCTT CATCTTCCTC TGTTCATTTC TGATGCTGAA TCTGTTCGTG
    GCTGTGATCA TGGATAATTT TGAATACCTG ACGCGTGACT CCTCTATCCT GGGACCCCAT
    CACCTGGACG AGTATGTGAG GATCTGGGCA GAATATGATC CTGCTGCTTG CGGGCGCATT
    CATTATAAGG ATATGTACAG TTTATTGCGA GTTATCGACC CCCCTCTGGG CTTAGGCAAG
    AAATGTCCTC ATAGGGTGGC CTGCAAGAGA CTGTTGCGGA TGGATCTGCC GGTTGCCGAT
    GACAACACAG TGCACTTTAA TTCTACCCTT ATGGCCTTAA TCCGAACCGC TCTGGACATC
    AAGATCGCCA AGGGTGGGGA AGGAGGTGTA GATAAGCACC AGATGGATGC TGAATTAAGG
    AAGGAGATGA TGGCGATATG GCCTAATCTC TCTCAGAAGA ACCTGGACCT GCTGGTGACT
    CCTCACAAGT CAGCGACAGA CCTGACAGTA GGAAAGATCT ACGCTGCCAT GATGATCATG
    GAGTATTATC GGCAAAGTAA AGCCAAACGG ACACAAGCTC TCCATGATGA GCAGAATCGA
    ACGCCATTAA TGTTTCAGCG TCTGGAGCCG CCATCTCCTA GCCAGGATGT GGGACCAGGA
    CTCACTGGCC TTCCTGACAC ACAAATGCAC CCTGCAAATC ATCTGCCTGT TGATGAAAGG
    ATCCCAGAGA GCCAATCATG GGTGACAGCG CGAGCACAGG AGATTTCTCA AAAAGCAGGC
    AACTGGAGTC CAGAGGGCCA ACATCCAGAT GACACCACAG ACAACAGAAG GCAGTCTCAG
    ACAGTAGAGA TGAGAGAGAT GGGGCGGGAC GGGTACTCGG ACACAGAGCA ATATCTGCCG
    ATGGAAGGCC ATGGCAGGGC TGCTTCCATG CCTCGACTCC CAGCAGACAA CCAACAGCCT
    ATCACAGATA ACAGTCCTAT GAAGCGCTCG GCTTCATCTT TGGGACACGG GCGTGCAGGA
    CGCTCCATGC GAGGTGAGGA TTATGCCATG GAGAGGGTTA TCCCAGAAGA GGGTCACAGG
    CATGGGCATC GCCACCGAGA CCGTAGTCAC CGGGCGTCAG AACGCTCCCT GAGCCGATAC
    ACTGATGCTG ACACAGGTCT TGGAACTGAC CTCAGCACAA CCACACAATC AGGAGACCTG
    CCATCCAAGG AGAGAGACCG GGGCCGAGCC AAAGACCGCA AGCACCATCA TCATCATCAT
    CACCACCACG GCTCTTTAGA TAAGGAGCAC TACGGTCATG AGAGAGACAG AGGAGAGTAC
    GGCCACAGAC AGTCCAGAGA GAGAGACCGC CGCTGGTCCC GCTCACCCAG TGAGGGCAGA
    GAGTGTCTGA CTCACAGACA GGGCAGTAGT TCAGTAAGCG GCAGTCCAGT CCCGTCCACC
    TCCGGGACCA GCACCCCCCG CCGTGGCCGC CGTCAGCTCC CCCAAACCCC TGCTACACCC
    CGGCCACATG TCACCTACTC ACCTGTGGTC CGTAAGCCCA TCAGCAGTAC CCCCCCTCCT
    GGCCAGCAGA GCCGACTCCC TACCCCGACC TCCCGGCGCT TCAGTCCGAC TGGCCCCGAG
    CCCCCGCTTC CCCCTCATCA CCACCCGTCA CCACCCCACC ACGGCTCTCC CCGCTCCGGC
    CGACATGCAC ACTGGGGCCC CGAGCCTGCA GAGAGCGTGG AGGGTGACGG CTTCTATGAC
    GACCAAGACT ATGAGTTCAA TCACCACGAG CCTCCATCCT ACGAGCAAAG CCCTGTGCAG
    GGCAACCCTC ACCCCCATTC CCCTAGGACT TCCCGCCACA ACACCCCTCC TCAGGGCCAA
    GCGCACCCAC GGCGCATGCC AAATGGCTAC CGTTCGTCTT CGCCCTCTCC ACACCACCAC
    GCGCCCGCTC ACCCTGGAAC TCATAAACCT CCTCATCCCA GAGGGCCCAG AAAGGGCCTC
    CATGAACCCT ACAGTGAGAC TGACGAGGAT GACTGGTGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017210286.1
    CDS1465..8526
    Translation

    Target ORF information:

    RefSeq Version XM_017354797.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017354797.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACAGGAAGC AGCAAAGGGA AAGACATCAG TACTATCAAG
    TCTCTACGTG TGCTTCGAGT ACTGCGGCCG CTGAAAACCA TCAAACGTCT GCCAAAACTG
    AAGGCTGTCT TTGACTGTGT GGTGAACTCT CTGAAGAATG TGTTGAACAT CCTCATCGTC
    TACATGCTCT TCATGTTCAT CTTCGCTGTG GTGGCTGTGC AGCTCTTTAA GGGACGTTTC
    TTTTACTGCA CTGATGAATC CAAAGAGTTT GAGCGCGACT GCAGGGGCGA GTATCTGGTC
    TATGAAAGAG ATAACGAAGT GAGGTCACAG AAGCGGGAAT GGAAGAAATA CGATTTCCAC
    TACGACAACG TGCTCTGGGC CCTTCTCACC CTCTTCACCG TTTCTACGGG AGAGGGGTGG
    CCGCAAGTGC TAAAACATTC AGTAGATGCG ACCTACGAGA ATCAGGGCCC GAGCCCAGGT
    TACCGGATGG AAATGTCCAT CTTTTACGTG GTGTACTTCG TGGTCTTCCC CTTCTTCTTC
    GTCAACATCT TCGTGGCTCT TATCATCATT ACTTTCCAGG AGCAAGGAGA CAAAATGATG
    GAGGACTATA GCCTGGAAAA GAATGAGAGA GCCTGCATAG ACTTTGCCAT CAACGCTAAG
    CCGCTCACAC GGCACATGCC CCAGAACAAG CAGACCTTTC AGTACCGCAT GTGGGAGTTT
    GTGGTGTCTC CTCCATTTGA ATACACCATC ATGGCCTTGA TCGCGCTCAA CACTATTGTC
    CTTATGATGA AGTATGATGG AGCATCGCTT ACTTATGAAG ATGTGCTAAA ATACCTGAAC
    ATAGTGTTCA CCTCTCTATT CTCCATGGAG TGCATATTGA AGATCATAGC CTTCGGAGCA
    TTGAACTACT TCAAAGATGC CTGGAACATC TTCGACTGTG TGACTGTACT TGGCAGCATC
    ACTGATATTT TAGTCACAGA GCTGGGGATG AACAACTTCA TAAATCTGAG TTTTCTAAGG
    CTCTTCAGAG CTGCTCGTCT CATTAAGTTG CTCAGGCAGG GTGAAACCAT ACGCATCCTG
    CTCTGGACTT TTGTCCAGTC TTTCAAGGCT CTGCCGTATG TGTGCCTACT CATCGCCATG
    CTGTTCTTCA TCTACGCCAT CATTGGCATG CAGCTGTTTG GGAACATTAA GATCGAGGAG
    AACAGTGACA GTGCCATTAC CCAGCACAAC AACTTCAGGA CTTTTTTCCA GGCCCTAATG
    CTGCTCTTTA GGAGTGCTAC AGGTGAGGCA TGGCATGACA TCATGCTGTC ATGTCTGGGA
    AAGAAGCCGT GTGATATTCT CTCTGATAAC CCGAAGCCAG AATGCGGGAG CGAGTTTGCC
    TACCTCTACT TTGTCTCCTT CATCTTCCTC TGTTCATTTC TGATGCTGAA TCTGTTCGTG
    GCTGTGATCA TGGATAATTT TGAATACCTG ACGCGTGACT CCTCTATCCT GGGACCCCAT
    CACCTGGACG AGTATGTGAG GATCTGGGCA GAATATGATC CTGCTGCTTG CGGGCGCATT
    CATTATAAGG ATATGTACAG TTTATTGCGA GTTATCGACC CCCCTCTGGG CTTAGGCAAG
    AAATGTCCTC ATAGGGTGGC CTGCAAGAGA CTGTTGCGGA TGGATCTGCC GGTTGCCGAT
    GACAACACAG TGCACTTTAA TTCTACCCTT ATGGCCTTAA TCCGAACCGC TCTGGACATC
    AAGATCGCCA AGGGTGGGGA AGGAGGTGTA GATAAGCACC AGATGGATGC TGAATTAAGG
    AAGGAGATGA TGGCGATATG GCCTAATCTC TCTCAGAAGA ACCTGGACCT GCTGGTGACT
    CCTCACAAGT CAGCGACAGA CCTGACAGTA GGAAAGATCT ACGCTGCCAT GATGATCATG
    GAGTATTATC GGCAAAGTAA AGCCAAACGG ACACAAGCTC TCCATGATGA GCAGAATCGA
    ACGCCATTAA TGTTTCAGCG TCTGGAGCCG CCATCTCCTA GCCAGGATGT GGGACCAGGA
    CTCACTGGCC TTCCTGACAC ACAAATGCAC CCTGCAAATC ATCTGCCTGT TGATGAAAGG
    ATCCCAGAGA GCCAATCATG GGTGACAGCG CGAGCACAGG AGATTTCTCA AAAAGCAGGC
    AACTGGAGTC CAGAGGGCCA ACATCCAGAT GACACCACAG ACAACAGAAG GCAGTCTCAG
    ACAGTAGAGA TGAGAGAGAT GGGGCGGGAC GGGTACTCGG ACACAGAGCA ATATCTGCCG
    ATGGAAGGCC ATGGCAGGGC TGCTTCCATG CCTCGACTCC CAGCAGACAA CCAACAGCCT
    ATCACAGATA ACAGTCCTAT GAAGCGCTCG GCTTCATCTT TGGGACACGG GCGTGCAGGA
    CGCTCCATGC GAGGTGAGGA TTATGCCATG GAGAGGGTTA TCCCAGAAGA GGGTCACAGG
    CATGGGCATC GCCACCGAGA CCGTAGTCAC CGGGCGTCAG AACGCTCCCT GAGCCGATAC
    ACTGATGCTG ACACAGGTCT TGGAACTGAC CTCAGCACAA CCACACAATC AGGAGACCTG
    CCATCCAAGG AGAGAGACCG GGGCCGAGCC AAAGACCGCA AGCACCATCA TCATCATCAT
    CACCACCACG GCTCTTTAGA TAAGGAGCAC TACGGTCATG AGAGAGACAG AGGAGAGTAC
    GGCCACAGAC AGTCCAGAGA GAGAGACCGC CGCTGGTCCC GCTCACCCAG TGAGGGCAGA
    GAGTGTCTGA CTCACAGACA GGGCAGTAGT TCAGTAAGCG GCAGTCCAGT CCCGTCCACC
    TCCGGGACCA GCACCCCCCG CCGTGGCCGC CGTCAGCTCC CCCAAACCCC TGCTACACCC
    CGGCCACATG TCACCTACTC ACCTGTGGTC CGTAAGCCCA TCAGCAGTAC CCCCCCTCCT
    GGCCAGCAGA GCCGACTCCC TACCCCGACC TCCCGGCGCT TCAGTCCGAC TGGCCCCGAG
    CCCCCGCTTC CCCCTCATCA CCACCCGTCA CCACCCCACC ACGGCTCTCC CCGCTCCGGC
    CGACATGCAC ACTGGGGCCC CGAGCCTGCA GAGAGCGTGG AGGGTGACGG CTTCTATGAC
    GACCAAGACT ATGAGTTCAA TCACCACGAG CCTCCATCCT ACGAGCAAAG CCCTGTGCAG
    GGCAACCCTC ACCCCCATTC CCCTAGGACT TCCCGCCACA ACACCCCTCC TCAGGGCCAA
    GCGCACCCAC GGCGCATGCC AAATGGCTAC CGTTCGTCTT CGCCCTCTCC ACACCACCAC
    GCGCCCGCTC ACCCTGGAAC TCATAAACCT CCTCATCCCA GAGGGCCCAG AAAGGGCCTC
    CATGAACCCT ACAGTGAGAC TGACGAGGAT GACTGGTGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa58254
    Clone ID Related Accession (Same CDS sequence) XM_017354798.1
    Accession Version XM_017354798.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7062bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGTGGCAGAA TCAGTTACAG GGATATGTAT GAGATGCTCA GGCACATGTC TCCTCCGTTA
    GGCCTGGGGA AGAAGTGCCC TGCACGGGTT GCCTACAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGAGGT GTAGATAAGC ACCAGATGGA TGCTGAATTA
    AGGAAGGAGA TGATGGCGAT ATGGCCTAAT CTCTCTCAGA AGAACCTGGA CCTGCTGGTG
    ACTCCTCACA AGTCGACAGA CCTGACAGTA GGAAAGATCT ACGCTGCCAT GATGATCATG
    GAGTATTATC GGCAAAGTAA AGCCAAACGG ACACAAGCTC TCCATGATGA GCAGAATCGA
    ACGCCATTAA TGTTTCAGCG TCTGGAGCCG CCATCTCCTA GCCAGGATGT GGGACCAGGA
    CTCACTGGCC TTCCTGACAC ACAAATGCAC CCTGCAAATC ATCTGCCTGT TGATGAAAGG
    ATCCCAGAGA GCCAATCATG GGTGACAGCG CGAGCACAGG AGATTTCTCA AAAAGCAGGC
    AACTGGAGTC CAGAGGGCCA ACATCCAGAT GACACCACAG ACAACAGAAG GCAGTCTCAG
    ACAGTAGAGA TGAGAGAGAT GGGGCGGGAC GGGTACTCGG ACACAGAGCA ATATCTGCCG
    ATGGAAGGCC ATGGCAGGGC TGCTTCCATG CCTCGACTCC CAGCAGACAA CCAACAGCCT
    ATCACAGATA ACAGTCCTAT GAAGCGCTCG GCTTCATCTT TGGGACACGG GCGTGCAGGA
    CGCTCCATGC GAGGTGAGGA TTATGCCATG GAGAGGGTTA TCCCAGAAGA GGGTCACAGG
    CATGGGCATC GCCACCGAGA CCGTAGTCAC CGGGCGTCAG AACGCTCCCT GAGCCGATAC
    ACTGATGCTG ACACAGGTCT TGGAACTGAC CTCAGCACAA CCACACAATC AGGAGACCTG
    CCATCCAAGG AGAGAGACCG GGGCCGAGCC AAAGACCGCA AGCACCATCA TCATCATCAT
    CACCACCACG GCTCTTTAGA TAAGGAGCAC TACGGTCATG AGAGAGACAG AGGAGAGTAC
    GGCCACAGAC AGTCCAGAGA GAGAGACCGC CGCTGGTCCC GCTCACCCAG TGAGGGCAGA
    GAGTGTCTGA CTCACAGACA GGGCAGTAGT TCAGTAAGCG GCAGTCCAGT CCCGTCCACC
    TCCGGGACCA GCACCCCCCG CCGTGGCCGC CGTCAGCTCC CCCAAACCCC TGCTACACCC
    CGGCCACATG TCACCTACTC ACCTGTGGTC CGTAAGCCCA TCAGCAGTAC CCCCCCTCCT
    GGCCAGCAGA GCCGACTCCC TACCCCGACC TCCCGGCGCT TCAGTCCGAC TGGCCCCGAG
    CCCCCGCTTC CCCCTCATCA CCACCCGTCA CCACCCCACC ACGGCTCTCC CCGCTCCGGC
    CGACATGCAC ACTGGGGCCC CGAGCCTGCA GAGAGCGTGG AGGGTGACGG CTTCTATGAC
    GACCAAGACT ATGAGTTCAA TCACCACGAG CCTCCATCCT ACGAGCAAAG CCCTGTGCAG
    GGCAACCCTC ACCCCCATTC CCCTAGGACT TCCCGCCACA ACACCCCTCC TCAGGGCCAA
    GCGCACCCAC GGCGCATGCC AAATGGCTAC CGTTCGTCTT CGCCCTCTCC ACACCACCAC
    GCGCCCGCTC ACCCTGGAAC TCATAAACCT CCTCATCCCA GAGGGCCCAG AAAGGGCCTC
    CATGAACCCT ACAGTGAGAC TGACGAGGAT GACTGGTGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017210287.1
    CDS1468..8529
    Translation

    Target ORF information:

    RefSeq Version XM_017354798.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017354798.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGTGGCAGAA TCAGTTACAG GGATATGTAT GAGATGCTCA GGCACATGTC TCCTCCGTTA
    GGCCTGGGGA AGAAGTGCCC TGCACGGGTT GCCTACAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGAGGT GTAGATAAGC ACCAGATGGA TGCTGAATTA
    AGGAAGGAGA TGATGGCGAT ATGGCCTAAT CTCTCTCAGA AGAACCTGGA CCTGCTGGTG
    ACTCCTCACA AGTCGACAGA CCTGACAGTA GGAAAGATCT ACGCTGCCAT GATGATCATG
    GAGTATTATC GGCAAAGTAA AGCCAAACGG ACACAAGCTC TCCATGATGA GCAGAATCGA
    ACGCCATTAA TGTTTCAGCG TCTGGAGCCG CCATCTCCTA GCCAGGATGT GGGACCAGGA
    CTCACTGGCC TTCCTGACAC ACAAATGCAC CCTGCAAATC ATCTGCCTGT TGATGAAAGG
    ATCCCAGAGA GCCAATCATG GGTGACAGCG CGAGCACAGG AGATTTCTCA AAAAGCAGGC
    AACTGGAGTC CAGAGGGCCA ACATCCAGAT GACACCACAG ACAACAGAAG GCAGTCTCAG
    ACAGTAGAGA TGAGAGAGAT GGGGCGGGAC GGGTACTCGG ACACAGAGCA ATATCTGCCG
    ATGGAAGGCC ATGGCAGGGC TGCTTCCATG CCTCGACTCC CAGCAGACAA CCAACAGCCT
    ATCACAGATA ACAGTCCTAT GAAGCGCTCG GCTTCATCTT TGGGACACGG GCGTGCAGGA
    CGCTCCATGC GAGGTGAGGA TTATGCCATG GAGAGGGTTA TCCCAGAAGA GGGTCACAGG
    CATGGGCATC GCCACCGAGA CCGTAGTCAC CGGGCGTCAG AACGCTCCCT GAGCCGATAC
    ACTGATGCTG ACACAGGTCT TGGAACTGAC CTCAGCACAA CCACACAATC AGGAGACCTG
    CCATCCAAGG AGAGAGACCG GGGCCGAGCC AAAGACCGCA AGCACCATCA TCATCATCAT
    CACCACCACG GCTCTTTAGA TAAGGAGCAC TACGGTCATG AGAGAGACAG AGGAGAGTAC
    GGCCACAGAC AGTCCAGAGA GAGAGACCGC CGCTGGTCCC GCTCACCCAG TGAGGGCAGA
    GAGTGTCTGA CTCACAGACA GGGCAGTAGT TCAGTAAGCG GCAGTCCAGT CCCGTCCACC
    TCCGGGACCA GCACCCCCCG CCGTGGCCGC CGTCAGCTCC CCCAAACCCC TGCTACACCC
    CGGCCACATG TCACCTACTC ACCTGTGGTC CGTAAGCCCA TCAGCAGTAC CCCCCCTCCT
    GGCCAGCAGA GCCGACTCCC TACCCCGACC TCCCGGCGCT TCAGTCCGAC TGGCCCCGAG
    CCCCCGCTTC CCCCTCATCA CCACCCGTCA CCACCCCACC ACGGCTCTCC CCGCTCCGGC
    CGACATGCAC ACTGGGGCCC CGAGCCTGCA GAGAGCGTGG AGGGTGACGG CTTCTATGAC
    GACCAAGACT ATGAGTTCAA TCACCACGAG CCTCCATCCT ACGAGCAAAG CCCTGTGCAG
    GGCAACCCTC ACCCCCATTC CCCTAGGACT TCCCGCCACA ACACCCCTCC TCAGGGCCAA
    GCGCACCCAC GGCGCATGCC AAATGGCTAC CGTTCGTCTT CGCCCTCTCC ACACCACCAC
    GCGCCCGCTC ACCCTGGAAC TCATAAACCT CCTCATCCCA GAGGGCCCAG AAAGGGCCTC
    CATGAACCCT ACAGTGAGAC TGACGAGGAT GACTGGTGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa58255
    Clone ID Related Accession (Same CDS sequence) XM_017354799.1
    Accession Version XM_017354799.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7050bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X11
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG
    GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT
    GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC
    CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC
    ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT
    GAGCAGCGTC TGGAGCCGCC ATCTCCTAGC CAGGATGTGG GACCAGGACT CACTGGCCTT
    CCTGACACAC AAATGCACCC TGCAAATCAT CTGCCTGTTG ATGAAAGGAT CCCAGAGAGC
    CAATCATGGG TGACAGCGCG AGCACAGGAG ATTTCTCAAA AAGCAGGCAA CTGGAGTCCA
    GAGGGCCAAC ATCCAGATGA CACCACAGAC AACAGAAGGC AGTCTCAGAC AGTAGAGATG
    AGAGAGATGG GGCGGGACGG GTACTCGGAC ACAGAGCAAT ATCTGCCGAT GGAAGGCCAT
    GGCAGGGCTG CTTCCATGCC TCGACTCCCA GCAGACAACC AACAGCCTAT CACAGATAAC
    AGTCCTATGA AGCGCTCGGC TTCATCTTTG GGACACGGGC GTGCAGGACG CTCCATGCGA
    GGTGAGGATT ATGCCATGGA GAGGGTTATC CCAGAAGAGG GTCACAGGCA TGGGCATCGC
    CACCGAGACC GTAGTCACCG GGCGTCAGAA CGCTCCCTGA GCCGATACAC TGATGCTGAC
    ACAGGTCTTG GAACTGACCT CAGCACAACC ACACAATCAG GAGACCTGCC ATCCAAGGAG
    AGAGACCGGG GCCGAGCCAA AGACCGCAAG CACCATCATC ATCATCATCA CCACCACGGC
    TCTTTAGATA AGGAGCACTA CGGTCATGAG AGAGACAGAG GAGAGTACGG CCACAGACAG
    TCCAGAGAGA GAGACCGCCG CTGGTCCCGC TCACCCAGTG AGGGCAGAGA GTGTCTGACT
    CACAGACAGG GCAGTAGTTC AGTAAGCGGC AGTCCAGTCC CGTCCACCTC CGGGACCAGC
    ACCCCCCGCC GTGGCCGCCG TCAGCTCCCC CAAACCCCTG CTACACCCCG GCCACATGTC
    ACCTACTCAC CTGTGGTCCG TAAGCCCATC AGCAGTACCC CCCCTCCTGG CCAGCAGAGC
    CGACTCCCTA CCCCGACCTC CCGGCGCTTC AGTCCGACTG GCCCCGAGCC CCCGCTTCCC
    CCTCATCACC ACCCGTCACC ACCCCACCAC GGCTCTCCCC GCTCCGGCCG ACATGCACAC
    TGGGGCCCCG AGCCTGCAGA GAGCGTGGAG GGTGACGGCT TCTATGACGA CCAAGACTAT
    GAGTTCAATC ACCACGAGCC TCCATCCTAC GAGCAAAGCC CTGTGCAGGG CAACCCTCAC
    CCCCATTCCC CTAGGACTTC CCGCCACAAC ACCCCTCCTC AGGGCCAAGC GCACCCACGG
    CGCATGCCAA ATGGCTACCG TTCGTCTTCG CCCTCTCCAC ACCACCACGC GCCCGCTCAC
    CCTGGAACTC ATAAACCTCC TCATCCCAGA GGGCCCAGAA AGGGCCTCCA TGAACCCTAC
    AGTGAGACTG ACGAGGATGA CTGGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017210288.1
    CDS1468..8517
    Translation

    Target ORF information:

    RefSeq Version XM_017354799.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017354799.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG
    GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT
    GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC
    CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC
    ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT
    GAGCAGCGTC TGGAGCCGCC ATCTCCTAGC CAGGATGTGG GACCAGGACT CACTGGCCTT
    CCTGACACAC AAATGCACCC TGCAAATCAT CTGCCTGTTG ATGAAAGGAT CCCAGAGAGC
    CAATCATGGG TGACAGCGCG AGCACAGGAG ATTTCTCAAA AAGCAGGCAA CTGGAGTCCA
    GAGGGCCAAC ATCCAGATGA CACCACAGAC AACAGAAGGC AGTCTCAGAC AGTAGAGATG
    AGAGAGATGG GGCGGGACGG GTACTCGGAC ACAGAGCAAT ATCTGCCGAT GGAAGGCCAT
    GGCAGGGCTG CTTCCATGCC TCGACTCCCA GCAGACAACC AACAGCCTAT CACAGATAAC
    AGTCCTATGA AGCGCTCGGC TTCATCTTTG GGACACGGGC GTGCAGGACG CTCCATGCGA
    GGTGAGGATT ATGCCATGGA GAGGGTTATC CCAGAAGAGG GTCACAGGCA TGGGCATCGC
    CACCGAGACC GTAGTCACCG GGCGTCAGAA CGCTCCCTGA GCCGATACAC TGATGCTGAC
    ACAGGTCTTG GAACTGACCT CAGCACAACC ACACAATCAG GAGACCTGCC ATCCAAGGAG
    AGAGACCGGG GCCGAGCCAA AGACCGCAAG CACCATCATC ATCATCATCA CCACCACGGC
    TCTTTAGATA AGGAGCACTA CGGTCATGAG AGAGACAGAG GAGAGTACGG CCACAGACAG
    TCCAGAGAGA GAGACCGCCG CTGGTCCCGC TCACCCAGTG AGGGCAGAGA GTGTCTGACT
    CACAGACAGG GCAGTAGTTC AGTAAGCGGC AGTCCAGTCC CGTCCACCTC CGGGACCAGC
    ACCCCCCGCC GTGGCCGCCG TCAGCTCCCC CAAACCCCTG CTACACCCCG GCCACATGTC
    ACCTACTCAC CTGTGGTCCG TAAGCCCATC AGCAGTACCC CCCCTCCTGG CCAGCAGAGC
    CGACTCCCTA CCCCGACCTC CCGGCGCTTC AGTCCGACTG GCCCCGAGCC CCCGCTTCCC
    CCTCATCACC ACCCGTCACC ACCCCACCAC GGCTCTCCCC GCTCCGGCCG ACATGCACAC
    TGGGGCCCCG AGCCTGCAGA GAGCGTGGAG GGTGACGGCT TCTATGACGA CCAAGACTAT
    GAGTTCAATC ACCACGAGCC TCCATCCTAC GAGCAAAGCC CTGTGCAGGG CAACCCTCAC
    CCCCATTCCC CTAGGACTTC CCGCCACAAC ACCCCTCCTC AGGGCCAAGC GCACCCACGG
    CGCATGCCAA ATGGCTACCG TTCGTCTTCG CCCTCTCCAC ACCACCACGC GCCCGCTCAC
    CCTGGAACTC ATAAACCTCC TCATCCCAGA GGGCCCAGAA AGGGCCTCCA TGAACCCTAC
    AGTGAGACTG ACGAGGATGA CTGGTGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa58256
    Clone ID Related Accession (Same CDS sequence) XM_017354800.1
    Accession Version XM_017354800.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7023bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X15
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCTAAGGA GCAGCAGAAG AACCACAAAG GTTGTAAGTC GGTATGGGAG
    CAGCGCACTA GTGAGTTAAG ACGACAGACC CTCGTGAACA GCAGAGAGGC TCTTTATAAT
    GAGTTGGACC CTGAAGACCG TTGGAAGGTC TCATACTCCC GTCACATACG TCCCGACATG
    AAAACTCACC TGGACCGGCC GTTAGTCGTG GACCCTCAGG AGAACCGCAA TAACAACACT
    AACAAAACCC GTCCGGGTGA CGGCCAACCT CAGCATTCCC ATCAGCTCCT ACACAAACAG
    CCCAACTTCA ATGAGGCCGA GACTGGAGGA GGTGGAGGGT CTGAGTCCTG GCCCGCACTG
    GAAAGAGGAG ACTCAACAGG GTCCCGCCGC AGTCTTTTGG GCTCTGAGCA CTATGAGGAG
    GGTAGAGAGT CCCACCGCAG ACACCAGCAC AGCCAGCACT GCAGTCAGGC CCGTCAACAT
    CGCAAAGCTG CAGAGGGCGG TGGAGAGCCT GGAGGGAGGA GACACAGGAG TAAGGCCAAG
    GAAAGAGGAC CTGAGTGTGA GCATTCAGAT GGAGGGGAGC GGAAACACAG ACGGCATCGG
    CATGAGGGTG AGGGGAGGAA AGAGAGAGGG GTACGACACC GAAACAGGAG GGATGGCCAT
    GCAAGTGGCC CGACTCTCTC TACAACCCGT CCCATTCAGA AGACTCTGTC CAGGCAGGAC
    AGTCAGTACA GCGAAGACCT GGACAATGCC ATGAACAACA AACTTGCTAC CCAGCCACAT
    GATAGTCTGC TTAATCTGGC CAACACTGCC CACTCTGCCG GCCTGGCACA TACTGGTACA
    GAGAGCAGCC TCATCCTCAC CAACCCTTCC TCTACCATTG CAAATGTTAC TAGCATCGGT
    CACCTGGGTA TGAAACCTGA GTACACTGCC GTGGACATTC CTCCCATGTT CCCCTCCAGT
    AATGCCATTC TACAGGTCAA TAAGAATGCC AACACGGAGC CTCTGCCAAA GAAGGAAGAC
    ACAAAGGGTG ACGATGATGA TGATGAGAAA GATGACGGAG GGCCTAAACC CATGCCACCC
    TACACCTCAA TGTTCATTCT GACCACCACC AACCCATTTC GGAGATTGTG TCACTATATC
    GTCACACTGA GATATTTTGA GATGTGTATT CTACTGGTTA TTGCTATGAG CAGTATTGCC
    CTGGCTGCTG AAGACCCTGT CTGGCCTGAA TCTCCCCGCA ACAATGTCCT ACGATACTTT
    GACTATGTGT TCACTGGTGT GTTCACCTTT GAGATGCTCA TTAAGATGGT AGATTTAGGT
    CTTGTCCTGC ATCAAGGCTC ATACTTTCGT GATCTGTGGA ACATCCTGGA CTTTATAGTG
    GTTAGTGGTG CCCTGGTGGC GTTCGCCTTC ACCGGAGGCA GCGGAGGAAG CAGCAAAGGG
    AAAGACATCA GTACTATCAA GTCTCTACGT GTGCTTCGAG TACTGCGGCC GCTGAAAACC
    ATCAAACGTC TGCCAAAACT GAAGGCTGTC TTTGACTGTG TGGTGAACTC TCTGAAGAAT
    GTGTTGAACA TCCTCATCGT CTACATGCTC TTCATGTTCA TCTTCGCTGT GGTGGCTGTG
    CAGCTCTTTA AGGGACGTTT CTTTTACTGC ACTGATGAAT CCAAAGAGTT TGAGCGCGAC
    TGCAGGGGCG AGTATCTGGT CTATGAAAGA GATAACGAAG TGAGGTCACA GAAGCGGGAA
    TGGAAGAAAT ACGATTTCCA CTACGACAAC GTGCTCTGGG CCCTTCTCAC CCTCTTCACC
    GTTTCTACGG GAGAGGGGTG GCCGCAAGTG CTAAAACATT CAGTAGATGC GACCTACGAG
    AATCAGGGCC CGAGCCCAGG TTACCGGATG GAAATGTCCA TCTTTTACGT GGTGTACTTC
    GTGGTCTTCC CCTTCTTCTT CGTCAACATC TTCGTGGCTC TTATCATCAT TACTTTCCAG
    GAGCAAGGAG ACAAAATGAT GGAGGACTAT AGCCTGGAAA AGAATGAGAG AGCCTGCATA
    GACTTTGCCA TCAACGCTAA GCCGCTCACA CGGCACATGC CCCAGAACAA GCAGACCTTT
    CAGTACCGCA TGTGGGAGTT TGTGGTGTCT CCTCCATTTG AATACACCAT CATGGCCTTG
    ATCGCGCTCA ACACTATTGT CCTTATGATG AAGTATGATG GAGCATCGCT TACTTATGAA
    GATGTGCTAA AATACCTGAA CATAGTGTTC ACCTCTCTAT TCTCCATGGA GTGCATATTG
    AAGATCATAG CCTTCGGAGC ATTGAACTAC TTCAAAGATG CCTGGAACAT CTTCGACTGT
    GTGACTGTAC TTGGCAGCAT CACTGATATT TTAGTCACAG AGCTGGGGAT GAACAACTTC
    ATAAATCTGA GTTTTCTAAG GCTCTTCAGA GCTGCTCGTC TCATTAAGTT GCTCAGGCAG
    GGTGAAACCA TACGCATCCT GCTCTGGACT TTTGTCCAGT CTTTCAAGGC TCTGCCGTAT
    GTGTGCCTAC TCATCGCCAT GCTGTTCTTC ATCTACGCCA TCATTGGCAT GCAGCTGTTT
    GGGAACATTA AGATCGAGGA GAACAGTGAC AGTGCCATTA CCCAGCACAA CAACTTCAGG
    ACTTTTTTCC AGGCCCTAAT GCTGCTCTTT AGGAGTGCTA CAGGTGAGGC ATGGCATGAC
    ATCATGCTGT CATGTCTGGG AAAGAAGCCG TGTGATATTC TCTCTGATAA CCCGAAGCCA
    GAATGCGGGA GCGAGTTTGC CTACCTCTAC TTTGTCTCCT TCATCTTCCT CTGTTCATTT
    CTGATGCTGA ATCTGTTCGT GGCTGTGATC ATGGATAATT TTGAATACCT GACGCGTGAC
    TCCTCTATCC TGGGACCCCA TCACCTGGAC GAGTATGTGA GGATCTGGGC AGAATATGAT
    CCTGCTGCTT GCGGGCGCAT TCATTATAAG GATATGTACA GTTTATTGCG AGTTATCGAC
    CCCCCTCTGG GCTTAGGCAA GAAATGTCCT CATAGGGTGG CCTGCAAGAG ACTGTTGCGG
    ATGGATCTGC CGGTTGCCGA TGACAACACA GTGCACTTTA ATTCTACCCT TATGGCCTTA
    ATCCGAACCG CTCTGGACAT CAAGATCGCC AAGGGTGGGG AAGGAGGTGT AGATAAGCAC
    CAGATGGATG CTGAATTAAG GAAGGAGATG ATGGCGATAT GGCCTAATCT CTCTCAGAAG
    AACCTGGACC TGCTGGTGAC TCCTCACAAG TCAGCGACAG ACCTGACAGT AGGAAAGATC
    TACGCTGCCA TGATGATCAT GGAGTATTAT CGGCAAAGTA AAGCCAAACG GACACAAGCT
    CTCCATGATG AGCAGAATCG AACGCCATTA ATGTTTCAGC GTCTGGAGCC GCCATCTCCT
    AGCCAGGATG TGGGACCAGG ACTCACTGGC CTTCCTGACA CACAAATGCA CCCTGCAAAT
    CATCTGCCTG TTGATGAAAG GATCCCAGAG AGCCAATCAT GGGTGACAGC GCGAGCACAG
    GAGATTTCTC AAAAAGCAGG CAACTGGAGT CCAGAGGGCC AACATCCAGA TGACACCACA
    GACAACAGAA GGCAGTCTCA GACAGTAGAG ATGAGAGAGA TGGGGCGGGA CGGGTACTCG
    GACACAGAGC AATATCTGCC GATGGAAGGC CATGGCAGGG CTGCTTCCAT GCCTCGACTC
    CCAGCAGACA ACCAACAGCC TATCACAGAT AACAGTCCTA TGAAGCGCTC GGCTTCATCT
    TTGGGACACG GGCGTGCAGG ACGCTCCATG CGAGGTGAGG ATTATGCCAT GGAGAGGGTT
    ATCCCAGAAG AGGGTCACAG GCATGGGCAT CGCCACCGAG ACCGTAGTCA CCGGGCGTCA
    GAACGCTCCC TGAGCCGATA CACTGATGCT GACACAGGTC TTGGAACTGA CCTCAGCACA
    ACCACACAAT CAGGAGACCT GCCATCCAAG GAGAGAGACC GGGGCCGAGC CAAAGACCGC
    AAGCACCATC ATCATCATCA TCACCACCAC GGCTCTTTAG ATAAGGAGCA CTACGGTCAT
    GAGAGAGACA GAGGAGAGTA CGGCCACAGA CAGTCCAGAG AGAGAGACCG CCGCTGGTCC
    CGCTCACCCA GTGAGGGCAG AGAGTGTCTG ACTCACAGAC AGGGCAGTAG TTCAGTAAGC
    GGCAGTCCAG TCCCGTCCAC CTCCGGGACC AGCACCCCCC GCCGTGGCCG CCGTCAGCTC
    CCCCAAACCC CTGCTACACC CCGGCCACAT GTCACCTACT CACCTGTGGT CCGTAAGCCC
    ATCAGCAGTA CCCCCCCTCC TGGCCAGCAG AGCCGACTCC CTACCCCGAC CTCCCGGCGC
    TTCAGTCCGA CTGGCCCCGA GCCCCCGCTT CCCCCTCATC ACCACCCGTC ACCACCCCAC
    CACGGCTCTC CCCGCTCCGG CCGACATGCA CACTGGGGCC CCGAGCCTGC AGAGAGCGTG
    GAGGGTGACG GCTTCTATGA CGACCAAGAC TATGAGTTCA ATCACCACGA GCCTCCATCC
    TACGAGCAAA GCCCTGTGCA GGGCAACCCT CACCCCCATT CCCCTAGGAC TTCCCGCCAC
    AACACCCCTC CTCAGGGCCA AGCGCACCCA CGGCGCATGC CAAATGGCTA CCGTTCGTCT
    TCGCCCTCTC CACACCACCA CGCGCCCGCT CACCCTGGAA CTCATAAACC TCCTCATCCC
    AGAGGGCCCA GAAAGGGCCT CCATGAACCC TACAGTGAGA CTGACGAGGA TGACTGGTGC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017210289.1
    CDS1466..8488
    Translation

    Target ORF information:

    RefSeq Version XM_017354800.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017354800.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCTAAGGA GCAGCAGAAG AACCACAAAG GTTGTAAGTC GGTATGGGAG
    CAGCGCACTA GTGAGTTAAG ACGACAGACC CTCGTGAACA GCAGAGAGGC TCTTTATAAT
    GAGTTGGACC CTGAAGACCG TTGGAAGGTC TCATACTCCC GTCACATACG TCCCGACATG
    AAAACTCACC TGGACCGGCC GTTAGTCGTG GACCCTCAGG AGAACCGCAA TAACAACACT
    AACAAAACCC GTCCGGGTGA CGGCCAACCT CAGCATTCCC ATCAGCTCCT ACACAAACAG
    CCCAACTTCA ATGAGGCCGA GACTGGAGGA GGTGGAGGGT CTGAGTCCTG GCCCGCACTG
    GAAAGAGGAG ACTCAACAGG GTCCCGCCGC AGTCTTTTGG GCTCTGAGCA CTATGAGGAG
    GGTAGAGAGT CCCACCGCAG ACACCAGCAC AGCCAGCACT GCAGTCAGGC CCGTCAACAT
    CGCAAAGCTG CAGAGGGCGG TGGAGAGCCT GGAGGGAGGA GACACAGGAG TAAGGCCAAG
    GAAAGAGGAC CTGAGTGTGA GCATTCAGAT GGAGGGGAGC GGAAACACAG ACGGCATCGG
    CATGAGGGTG AGGGGAGGAA AGAGAGAGGG GTACGACACC GAAACAGGAG GGATGGCCAT
    GCAAGTGGCC CGACTCTCTC TACAACCCGT CCCATTCAGA AGACTCTGTC CAGGCAGGAC
    AGTCAGTACA GCGAAGACCT GGACAATGCC ATGAACAACA AACTTGCTAC CCAGCCACAT
    GATAGTCTGC TTAATCTGGC CAACACTGCC CACTCTGCCG GCCTGGCACA TACTGGTACA
    GAGAGCAGCC TCATCCTCAC CAACCCTTCC TCTACCATTG CAAATGTTAC TAGCATCGGT
    CACCTGGGTA TGAAACCTGA GTACACTGCC GTGGACATTC CTCCCATGTT CCCCTCCAGT
    AATGCCATTC TACAGGTCAA TAAGAATGCC AACACGGAGC CTCTGCCAAA GAAGGAAGAC
    ACAAAGGGTG ACGATGATGA TGATGAGAAA GATGACGGAG GGCCTAAACC CATGCCACCC
    TACACCTCAA TGTTCATTCT GACCACCACC AACCCATTTC GGAGATTGTG TCACTATATC
    GTCACACTGA GATATTTTGA GATGTGTATT CTACTGGTTA TTGCTATGAG CAGTATTGCC
    CTGGCTGCTG AAGACCCTGT CTGGCCTGAA TCTCCCCGCA ACAATGTCCT ACGATACTTT
    GACTATGTGT TCACTGGTGT GTTCACCTTT GAGATGCTCA TTAAGATGGT AGATTTAGGT
    CTTGTCCTGC ATCAAGGCTC ATACTTTCGT GATCTGTGGA ACATCCTGGA CTTTATAGTG
    GTTAGTGGTG CCCTGGTGGC GTTCGCCTTC ACCGGAGGCA GCGGAGGAAG CAGCAAAGGG
    AAAGACATCA GTACTATCAA GTCTCTACGT GTGCTTCGAG TACTGCGGCC GCTGAAAACC
    ATCAAACGTC TGCCAAAACT GAAGGCTGTC TTTGACTGTG TGGTGAACTC TCTGAAGAAT
    GTGTTGAACA TCCTCATCGT CTACATGCTC TTCATGTTCA TCTTCGCTGT GGTGGCTGTG
    CAGCTCTTTA AGGGACGTTT CTTTTACTGC ACTGATGAAT CCAAAGAGTT TGAGCGCGAC
    TGCAGGGGCG AGTATCTGGT CTATGAAAGA GATAACGAAG TGAGGTCACA GAAGCGGGAA
    TGGAAGAAAT ACGATTTCCA CTACGACAAC GTGCTCTGGG CCCTTCTCAC CCTCTTCACC
    GTTTCTACGG GAGAGGGGTG GCCGCAAGTG CTAAAACATT CAGTAGATGC GACCTACGAG
    AATCAGGGCC CGAGCCCAGG TTACCGGATG GAAATGTCCA TCTTTTACGT GGTGTACTTC
    GTGGTCTTCC CCTTCTTCTT CGTCAACATC TTCGTGGCTC TTATCATCAT TACTTTCCAG
    GAGCAAGGAG ACAAAATGAT GGAGGACTAT AGCCTGGAAA AGAATGAGAG AGCCTGCATA
    GACTTTGCCA TCAACGCTAA GCCGCTCACA CGGCACATGC CCCAGAACAA GCAGACCTTT
    CAGTACCGCA TGTGGGAGTT TGTGGTGTCT CCTCCATTTG AATACACCAT CATGGCCTTG
    ATCGCGCTCA ACACTATTGT CCTTATGATG AAGTATGATG GAGCATCGCT TACTTATGAA
    GATGTGCTAA AATACCTGAA CATAGTGTTC ACCTCTCTAT TCTCCATGGA GTGCATATTG
    AAGATCATAG CCTTCGGAGC ATTGAACTAC TTCAAAGATG CCTGGAACAT CTTCGACTGT
    GTGACTGTAC TTGGCAGCAT CACTGATATT TTAGTCACAG AGCTGGGGAT GAACAACTTC
    ATAAATCTGA GTTTTCTAAG GCTCTTCAGA GCTGCTCGTC TCATTAAGTT GCTCAGGCAG
    GGTGAAACCA TACGCATCCT GCTCTGGACT TTTGTCCAGT CTTTCAAGGC TCTGCCGTAT
    GTGTGCCTAC TCATCGCCAT GCTGTTCTTC ATCTACGCCA TCATTGGCAT GCAGCTGTTT
    GGGAACATTA AGATCGAGGA GAACAGTGAC AGTGCCATTA CCCAGCACAA CAACTTCAGG
    ACTTTTTTCC AGGCCCTAAT GCTGCTCTTT AGGAGTGCTA CAGGTGAGGC ATGGCATGAC
    ATCATGCTGT CATGTCTGGG AAAGAAGCCG TGTGATATTC TCTCTGATAA CCCGAAGCCA
    GAATGCGGGA GCGAGTTTGC CTACCTCTAC TTTGTCTCCT TCATCTTCCT CTGTTCATTT
    CTGATGCTGA ATCTGTTCGT GGCTGTGATC ATGGATAATT TTGAATACCT GACGCGTGAC
    TCCTCTATCC TGGGACCCCA TCACCTGGAC GAGTATGTGA GGATCTGGGC AGAATATGAT
    CCTGCTGCTT GCGGGCGCAT TCATTATAAG GATATGTACA GTTTATTGCG AGTTATCGAC
    CCCCCTCTGG GCTTAGGCAA GAAATGTCCT CATAGGGTGG CCTGCAAGAG ACTGTTGCGG
    ATGGATCTGC CGGTTGCCGA TGACAACACA GTGCACTTTA ATTCTACCCT TATGGCCTTA
    ATCCGAACCG CTCTGGACAT CAAGATCGCC AAGGGTGGGG AAGGAGGTGT AGATAAGCAC
    CAGATGGATG CTGAATTAAG GAAGGAGATG ATGGCGATAT GGCCTAATCT CTCTCAGAAG
    AACCTGGACC TGCTGGTGAC TCCTCACAAG TCAGCGACAG ACCTGACAGT AGGAAAGATC
    TACGCTGCCA TGATGATCAT GGAGTATTAT CGGCAAAGTA AAGCCAAACG GACACAAGCT
    CTCCATGATG AGCAGAATCG AACGCCATTA ATGTTTCAGC GTCTGGAGCC GCCATCTCCT
    AGCCAGGATG TGGGACCAGG ACTCACTGGC CTTCCTGACA CACAAATGCA CCCTGCAAAT
    CATCTGCCTG TTGATGAAAG GATCCCAGAG AGCCAATCAT GGGTGACAGC GCGAGCACAG
    GAGATTTCTC AAAAAGCAGG CAACTGGAGT CCAGAGGGCC AACATCCAGA TGACACCACA
    GACAACAGAA GGCAGTCTCA GACAGTAGAG ATGAGAGAGA TGGGGCGGGA CGGGTACTCG
    GACACAGAGC AATATCTGCC GATGGAAGGC CATGGCAGGG CTGCTTCCAT GCCTCGACTC
    CCAGCAGACA ACCAACAGCC TATCACAGAT AACAGTCCTA TGAAGCGCTC GGCTTCATCT
    TTGGGACACG GGCGTGCAGG ACGCTCCATG CGAGGTGAGG ATTATGCCAT GGAGAGGGTT
    ATCCCAGAAG AGGGTCACAG GCATGGGCAT CGCCACCGAG ACCGTAGTCA CCGGGCGTCA
    GAACGCTCCC TGAGCCGATA CACTGATGCT GACACAGGTC TTGGAACTGA CCTCAGCACA
    ACCACACAAT CAGGAGACCT GCCATCCAAG GAGAGAGACC GGGGCCGAGC CAAAGACCGC
    AAGCACCATC ATCATCATCA TCACCACCAC GGCTCTTTAG ATAAGGAGCA CTACGGTCAT
    GAGAGAGACA GAGGAGAGTA CGGCCACAGA CAGTCCAGAG AGAGAGACCG CCGCTGGTCC
    CGCTCACCCA GTGAGGGCAG AGAGTGTCTG ACTCACAGAC AGGGCAGTAG TTCAGTAAGC
    GGCAGTCCAG TCCCGTCCAC CTCCGGGACC AGCACCCCCC GCCGTGGCCG CCGTCAGCTC
    CCCCAAACCC CTGCTACACC CCGGCCACAT GTCACCTACT CACCTGTGGT CCGTAAGCCC
    ATCAGCAGTA CCCCCCCTCC TGGCCAGCAG AGCCGACTCC CTACCCCGAC CTCCCGGCGC
    TTCAGTCCGA CTGGCCCCGA GCCCCCGCTT CCCCCTCATC ACCACCCGTC ACCACCCCAC
    CACGGCTCTC CCCGCTCCGG CCGACATGCA CACTGGGGCC CCGAGCCTGC AGAGAGCGTG
    GAGGGTGACG GCTTCTATGA CGACCAAGAC TATGAGTTCA ATCACCACGA GCCTCCATCC
    TACGAGCAAA GCCCTGTGCA GGGCAACCCT CACCCCCATT CCCCTAGGAC TTCCCGCCAC
    AACACCCCTC CTCAGGGCCA AGCGCACCCA CGGCGCATGC CAAATGGCTA CCGTTCGTCT
    TCGCCCTCTC CACACCACCA CGCGCCCGCT CACCCTGGAA CTCATAAACC TCCTCATCCC
    AGAGGGCCCA GAAAGGGCCT CCATGAACCC TACAGTGAGA CTGACGAGGA TGACTGGTGC
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa58257
    Clone ID Related Accession (Same CDS sequence) XM_017354801.1
    Accession Version XM_017354801.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6456bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product voltage-dependent P/Q-type calcium channel subunit alpha-1A isoform X18
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG
    GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT
    GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC
    CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC
    ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT
    GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT
    GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT
    GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT
    CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA
    AGGCAGTCTC AGACAGTAGA GATGAGAGAG ATGGGGCGGG ACGGGTACTC GGACACAGAG
    CAATATCTGC CGATGGAAGG CCATGGCAGG GCTGCTTCCA TGCCTCGACT CCCAGCAGAC
    AACCAACAGC CTATCACAGA TAACAGTCCT ATGAAGCGCT CGGCTTCATC TTTGGGACAC
    GGGCGTGCAG GACGCTCCAT GCGAGGTGAG GATTATGCCA TGGAGAGGGT TATCCCAGAA
    GAGGGTCACA GGCATGGGCA TCGCCACCGA GACCGTAGTC ACCGGGCGTC AGAACGCTCC
    CTGAGCCGAT ACACTGATGC TGACACAGGT CTTGGAACTG ACCTCAGCAC AACCACACAA
    TCAGGAGACC TGCCATCCAA GGAGAGAGAC CGGGGCCGAG CCAAAGACCG CAAGCACCAT
    CATCATCATC ATCACCACCA CGGCTCTTTA GATAAGGAGC ACTACGGTCA TGAGAGAGAC
    AGAGGAGAGT ACGGCCACAG ACAGTCCAGA GAGAGAGACC GCCGCTGGTC CCGCTCACCC
    AGTGAGGGCA GAGAGTGTCT GACTCACAGA CAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017210290.1
    CDS1474..7929
    Translation

    Target ORF information:

    RefSeq Version XM_017354801.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, a (cacna1aa), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017354801.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGGCTCGGT TCGGAGATGA GGTGCCGTCC AGGTATGGCG GAGGACCGGG ACACGCTCAA 
    GGGGGCCCCG GCCGCGGCGG CAGCAGGCAG GGCGGCCCGC CGGGGGCTCA GCAGAGAATG
    TACAAGCAGT CAATGGCCCA GCGAGCCAGA ACTATGGCCC TGTACAACCC TATACCGGTG
    CGGCAAAACT GCTTTACTGT CAACCGATCT CTGTTCATCT TCAGTGAGGA CAACTTCGTG
    AGAAAATACG CCAAAAAGAT CACCGAATGG CCTCCATTTG AGTACATGAT CCTGGCAACC
    ATCATCGCTA ACTGCATTGT CCTGGCCCTG GAACAGCACC TTCCAGACGG GGACAAGACA
    CCTATGTCAG AGCGTTTGGA GGACACAGAA CCCTACTTCA TTGGCATTTT CTGTTTCGAG
    TCAGGCATTA AGATATTAGC TCTGGGATTT GCCTTTCACA AGGGCTCCTA CCTACGAAAT
    GGCTGGAATG TGATGGACTT TGTAGTGGTG CTTACTGGAA TCCTGTCCAC CGTGGGCTCA
    GACTTTGACC TGAGAACTCT ACGTGCTGTT CGAGTGTTGA GACCTCTCAA GCTGGTGTCT
    GGGATTCCCA GCTTACAGGT TGTGCTGAAA TCCATCATGA AGGCCATGAT CCCTCTGCTT
    CAGATCGGTT TGCTGCTCTT CTTTGCCATC CTCATGTTCG CAATCATTGG TCTAGAGTTC
    TACATGGGCA AATTTCACAC CACTTGCTTT GACAAGATCA CCGATGAGAT CCGGGAGGAG
    TTTCCATGTG GGGAGGAGGT TCCCGCTCGA ATATGTCCTA ATGGAACCGT ATGCAAAAAG
    TACTGGCTTG GACCGAACTA TGGTATTACT CAGTTTGACA ACATCCTGTT TGCCGTCCTC
    ACAGTCTTTC AGTGCATCAC CATGGAGGGC TGGACTGATC TGCTGTACTA TAGCAATGAT
    GCTGCAGGGA GTGCATGGAA CTGGATGTAC TTCATCCCGC TCATCATCAT TGGCTCTTTC
    TTCATGCTCA ATCTTGTGCT GGGTGTGCTG TCAGGAGAGT TTGCCAAAGA AAGGGAGCGT
    GTGGAGAACA GGAGCGAGTT CCTAAAGCTG AAGCGTCAGC AGCAGATAGA GAGAGAGCTG
    AACGGATATC TGGAGTGGAT CTGCAAAGCA GAAGAAGTGA TTCTGGCTGA CGAGGACAAT
    GACCCAGATG ACCGAATGCC CTTTGATGGC TCGAGAAGGA GGCCCACCAT CAAGAAGAGT
    AAAACAGACC TGTTGGATGC AGAGGATGGA GATGGAGATA TAGGTTCTCC GTTTGCACGG
    GGCAGTCTGA AAAGCTCCAA ATTGGAGGGC TCTTCCTTCC ATAAAAAGGA GCGTCGGCTA
    AGATTCTTCA TTAGGCGTAT TGTGAAGACT CAAGCATTCT ACTGGACTGT TCTGTGTCTG
    GTGGGACTCA ACACACTGTG TGTGGCTGTG GTTCATTACG ACCAGCCCGA AACGCTCTCT
    GACTTCCTTT ACTTTGCTGA GTTCATCTTT CTGGGCATCT TTATGTCAGA GATGTGTATT
    AAGATGTACG GACTGGGCAC CAGACCTTAT TTTCACTCCT CCTTCAACTG TTTTGACTGC
    ATTGTCATTT GTGGCAGCAT TTTCGAGGTG CTCTGGGCTA TGATCCAGCC AGGAACCTCA
    TTTGGGATCA GTGTGTTACG AGCTCTCAGG TTACTGAGGA TCTTCAAAGT GACCAAGTAT
    TGGGCGTCTC TGAGGAACCT GGTGGTGTCT TTGCTAAATT CCATGAAGTC CATTATCAGT
    TTACTCTTCC TCCTTTTCCT CTTCATTGTG GTCTTTGCTT TGCTGGGGAT GCAGCTGTTT
    GGGGGACAGT TTAATTTTGA AGCCGGCACA CCCCCAACAA ACTTTGATAC TTTTCCTGCA
    GCAATAATGA CAGTGTTTCA GATCCTGACC GGTGAAGACT GGAACATGGT GATGTATGAT
    GGGATCGAGT CTCAGGGTGG AGTCAAAAAG GGCATGGTCT TCTCAGTCTT CTTCATCGTT
    CTTACGCTCT TCGGCAACTA CACACTGCTC AATGTGTTTT TGGCTATCGC TGTGGATAAC
    CTGGCCAATG CACAAGAGCT GACCAAGGAT GAACAGGAGG AAGAGCAGGC AGCCAATAAG
    AAAATGGCTC TTCAGACGGC CAAGGAAGTG GCTGAGGTCA GCCCTCTGTC TGCAGCAAAC
    CTCTCTATCG CAGCCGTGAC AGTAAACTCT GAGCGTCTCG ATGCAACAGA ACACTTTCTA
    GACTGTAAGG AGCAGCAGAA GAACCACAAA GGTTGTAAGT CGGTATGGGA GCAGCGCACT
    AGTGAGTTAA GACGACAGAC CCTCGTGAAC AGCAGAGAGG CTCTTTATAA TGAGTTGGAC
    CCTGAAGACC GTTGGAAGGT CTCATACTCC CGTCACATAC GTCCCGACAT GAAAACTCAC
    CTGGACCGGC CGTTAGTCGT GGACCCTCAG GAGAACCGCA ATAACAACAC TAACAAAACC
    CGTCCGGGTG ACGGCCAACC TCAGCATTCC CATCAGCTCC TACACAAACA GCCCAACTTC
    AATGAGGCCG AGACTGGAGG AGGTGGAGGG TCTGAGTCCT GGCCCGCACT GGAAAGAGGA
    GACTCAACAG GGTCCCGCCG CAGTCTTTTG GGCTCTGAGC ACTATGAGGA GGGTAGAGAG
    TCCCACCGCA GACACCAGCA CAGCCAGCAC TGCAGTCAGG CCCGTCAACA TCGCAAAGCT
    GCAGAGGGCG GTGGAGAGCC TGGAGGGAGG AGACACAGGA GTAAGGCCAA GGAAAGAGGA
    CCTGAGTGTG AGCATTCAGA TGGAGGGGAG CGGAAACACA GACGGCATCG GCATGAGGGT
    GAGGGGAGGA AAGAGAGAGG GGTACGACAC CGAAACAGGA GGGATGGCCA TGCAAGTGGC
    CCGACTCTCT CTACAACCCG TCCCATTCAG AAGACTCTGT CCAGGCAGGA CAGTCAGTAC
    AGCGAAGACC TGGACAATGC CATGAACAAC AAACTTGCTA CCCAGCCACA TGATAGTCTG
    CTTAATCTGG CCAACACTGC CCACTCTGCC GGCCTGGCAC ATACTGGTAC AGAGAGCAGC
    CTCATCCTCA CCAACCCTTC CTCTACCATT GCAAATGTTA CTAGCATCGG TCACCTGGGT
    ATGAAACCTG AGTACACTGC CGTGGACATT CCTCCCATGT TCCCCTCCAG TAATGCCATT
    CTACAGGTCA ATAAGAATGC CAACACGGAG CCTCTGCCAA AGAAGGAAGA CACAAAGGGT
    GACGATGATG ATGATGAGAA AGATGACGGA GGGCCTAAAC CCATGCCACC CTACACCTCA
    ATGTTCATTC TGACCACCAC CAACCCATTT CGGAGATTGT GTCACTATAT CGTCACACTG
    AGATATTTTG AGATGTGTAT TCTACTGGTT ATTGCTATGA GCAGTATTGC CCTGGCTGCT
    GAAGACCCTG TCTGGCCTGA ATCTCCCCGC AACAATGTCC TACGATACTT TGACTATGTG
    TTCACTGGTG TGTTCACCTT TGAGATGCTC ATTAAGATGG TAGATTTAGG TCTTGTCCTG
    CATCAAGGCT CATACTTTCG TGATCTGTGG AACATCCTGG ACTTTATAGT GGTTAGTGGT
    GCCCTGGTGG CGTTCGCCTT CACCGGAGGC AGCGGAGGAA GCAGCAAAGG GAAAGACATC
    AGTACTATCA AGTCTCTACG TGTGCTTCGA GTACTGCGGC CGCTGAAAAC CATCAAACGT
    CTGCCAAAAC TGAAGGCTGT CTTTGACTGT GTGGTGAACT CTCTGAAGAA TGTGTTGAAC
    ATCCTCATCG TCTACATGCT CTTCATGTTC ATCTTCGCTG TGGTGGCTGT GCAGCTCTTT
    AAGGGACGTT TCTTTTACTG CACTGATGAA TCCAAAGAGT TTGAGCGCGA CTGCAGGGGC
    GAGTATCTGG TCTATGAAAG AGATAACGAA GTGAGGTCAC AGAAGCGGGA ATGGAAGAAA
    TACGATTTCC ACTACGACAA CGTGCTCTGG GCCCTTCTCA CCCTCTTCAC CGTTTCTACG
    GGAGAGGGGT GGCCGCAAGT GCTAAAACAT TCAGTAGATG CGACCTACGA GAATCAGGGC
    CCGAGCCCAG GTTACCGGAT GGAAATGTCC ATCTTTTACG TGGTGTACTT CGTGGTCTTC
    CCCTTCTTCT TCGTCAACAT CTTCGTGGCT CTTATCATCA TTACTTTCCA GGAGCAAGGA
    GACAAAATGA TGGAGGACTA TAGCCTGGAA AAGAATGAGA GAGCCTGCAT AGACTTTGCC
    ATCAACGCTA AGCCGCTCAC ACGGCACATG CCCCAGAACA AGCAGACCTT TCAGTACCGC
    ATGTGGGAGT TTGTGGTGTC TCCTCCATTT GAATACACCA TCATGGCCTT GATCGCGCTC
    AACACTATTG TCCTTATGAT GAAGTATGAT GGAGCATCGC TTACTTATGA AGATGTGCTA
    AAATACCTGA ACATAGTGTT CACCTCTCTA TTCTCCATGG AGTGCATATT GAAGATCATA
    GCCTTCGGAG CATTGAACTA CTTCAAAGAT GCCTGGAACA TCTTCGACTG TGTGACTGTA
    CTTGGCAGCA TCACTGATAT TTTAGTCACA GAGCTGGGGA TGAACAACTT CATAAATCTG
    AGTTTTCTAA GGCTCTTCAG AGCTGCTCGT CTCATTAAGT TGCTCAGGCA GGGTGAAACC
    ATACGCATCC TGCTCTGGAC TTTTGTCCAG TCTTTCAAGG CTCTGCCGTA TGTGTGCCTA
    CTCATCGCCA TGCTGTTCTT CATCTACGCC ATCATTGGCA TGCAGCTGTT TGGGAACATT
    AAGATCGAGG AGAACAGTGA CAGTGCCATT ACCCAGCACA ACAACTTCAG GACTTTTTTC
    CAGGCCCTAA TGCTGCTCTT TAGGAGTGCT ACAGGTGAGG CATGGCATGA CATCATGCTG
    TCATGTCTGG GAAAGAAGCC GTGTGATATT CTCTCTGATA ACCCGAAGCC AGAATGCGGG
    AGCGAGTTTG CCTACCTCTA CTTTGTCTCC TTCATCTTCC TCTGTTCATT TCTGATGCTG
    AATCTGTTCG TGGCTGTGAT CATGGATAAT TTTGAATACC TGACGCGTGA CTCCTCTATC
    CTGGGACCCC ATCACCTGGA CGAGTATGTG AGGATCTGGG CAGAATATGA TCCTGCTGCT
    TGCGGGCGCA TTCATTATAA GGATATGTAC AGTTTATTGC GAGTTATCGA CCCCCCTCTG
    GGCTTAGGCA AGAAATGTCC TCATAGGGTG GCCTGCAAGA GACTGTTGCG GATGGATCTG
    CCGGTTGCCG ATGACAACAC AGTGCACTTT AATTCTACCC TTATGGCCTT AATCCGAACC
    GCTCTGGACA TCAAGATCGC CAAGGGTGGG GAAGGAGGTG TAGATAAGCA CCAGATGGAT
    GCTGAATTAA GGAAGGAGAT GATGGCGATA TGGCCTAATC TCTCTCAGAA GAACCTGGAC
    CTGCTGGTGA CTCCTCACAA GTCAGCGACA GACCTGACAG TAGGAAAGAT CTACGCTGCC
    ATGATGATCA TGGAGTATTA TCGGCAAAGT AAAGCCAAAC GGACACAAGC TCTCCATGAT
    GAGCAGAATC GAACGCCATT AATGTTTCAG CGTCTGGAGC CGCCATCTCC TAGCCAGGAT
    GTGGGACCAG GACTCACTGG CCTTCCTGAC ACACAAATGC ACCCTGCAAA TCATCTGCCT
    GTTGATGAAA GGATCCCAGA GAGCCAATCA TGGGTGACAG CGCGAGCACA GGAGATTTCT
    CAAAAAGCAG GCAACTGGAG TCCAGAGGGC CAACATCCAG ATGACACCAC AGACAACAGA
    AGGCAGTCTC AGACAGTAGA GATGAGAGAG ATGGGGCGGG ACGGGTACTC GGACACAGAG
    CAATATCTGC CGATGGAAGG CCATGGCAGG GCTGCTTCCA TGCCTCGACT CCCAGCAGAC
    AACCAACAGC CTATCACAGA TAACAGTCCT ATGAAGCGCT CGGCTTCATC TTTGGGACAC
    GGGCGTGCAG GACGCTCCAT GCGAGGTGAG GATTATGCCA TGGAGAGGGT TATCCCAGAA
    GAGGGTCACA GGCATGGGCA TCGCCACCGA GACCGTAGTC ACCGGGCGTC AGAACGCTCC
    CTGAGCCGAT ACACTGATGC TGACACAGGT CTTGGAACTG ACCTCAGCAC AACCACACAA
    TCAGGAGACC TGCCATCCAA GGAGAGAGAC CGGGGCCGAG CCAAAGACCG CAAGCACCAT
    CATCATCATC ATCACCACCA CGGCTCTTTA GATAAGGAGC ACTACGGTCA TGAGAGAGAC
    AGAGGAGAGT ACGGCCACAG ACAGTCCAGA GAGAGAGACC GCCGCTGGTC CCGCTCACCC
    AGTGAGGGCA GAGAGTGTCT GACTCACAGA CAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.