nlgn4a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	nlgn4a
Entrez Gene ID	561122
Full Name	neuroligin 4a
Synonyms	nlgn4b
General protein information	Preferred Names neuroligin 4a Names neuroligin-4, X-linked neuroligin 4b
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009292171.2	XP_009290446.1	neuroligin-4, X-linked isoform X1
XM_021475240.1	XP_021330915.1	neuroligin-4, X-linked isoform X1
XM_009292174.2	XP_009290449.1	neuroligin-4, X-linked isoform X2
NM_001166331.1	NP_001159803.1	neuroligin-4, X-linked
XM_009292174.3	XP_009290449.1	neuroligin-4, X-linked isoform X2

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_00499	XP_003718562.1
Neurospora crassa	NCU02679	XP_965452.1
Pan troglodytes (chimpanzee)	NLGN4X	XP_001138543.2
Homo sapiens (human)	NLGN4X	NP_851849.1
Danio rerio (zebrafish)	nlgn4a	NP_001159803.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Characterization of the neuroligin gene family expression and evolution in zebrafish.
Rissone A, Sangiorgio L, Monopoli M, Beltrame M, Zucchi I, Bussolino F, Arese M, Cotelli F
Developmental dynamics : an official publication of the American Association of Anatomists239(2)688-702(2010 Feb)

Differential expression of neuroligin genes in the nervous system of zebrafish.
Davey C, Tallafuss A, Washbourne P
Developmental dynamics : an official publication of the American Association of Anatomists239(2)703-14(2010 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following nlgn4a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nlgn4a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa04902	NM_001166331.1 Latest version!	Danio rerio neuroligin 4 X-linked a (nlgn4xa), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa43431	XM_009292171.2 Latest version!	Danio rerio neuroligin 4a (nlgn4a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa43432	XM_009292174.3 Latest version!	Danio rerio neuroligin 4a (nlgn4a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa43430	XM_021475240.1 Latest version!	Danio rerio neuroligin 4a (nlgn4a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
ODa43432	XM_009292174.2	Danio rerio neuroligin 4a (nlgn4a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nlgn4a ( NM_001166331.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001159803.1 Danio rerio neuroligin 4 X-linked a (nlgn4xa), mRNA.

Price & Availability↑

CloneID	ODa04902
Clone ID Related Accession (Same CDS sequence)	NM_001166331.1
Accession Version	NM_001166331.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2481bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	neuroligin-4, X-linked
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from GQ892839.1. On Oct 21, 2009 this sequence version replaced XM_684526.3. ##Evidence-Data-START## Transcript exon combination :: GQ892839.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505370, SAMEA3505371 [ECO:0000348] ##Evidence-Data-END##

ATGTCACGGC TCGGAGGAAC CCCATGGATA CCACTTACCA TGGTTCATCC GCTGGCTACT 
CTACGCCTTT TTACCTGGAT TGTGCTTGCG GCTGCGTGGC TGGCCATTAC ACGGGCTCAG 
CAGCACCCTA TTGTTACGAC AAACTATGGC AAGCTAAGAG GCTTGAAAAC ACCTCTGCCC 
AATGAGATTC TAGGGCCCGT GGAACAGTAT TTGGGGATCC CCTATGCCCT GCCGCCCACA 
GGAGAAAGAA GGTTTCAGCC CCCAGAGCCT CCAATGTCCT GGCCAGGGAT TCGAAATGCT 
ACACAGTTTG CTCCTGTTTG TCCTCAGTTT CTGGAAGATC GTTTTTTACT CAATGATATG 
TTACCCGTGT GGTTCACCGC CAACTTGGAT ACTGTTGTAA CCTACGTACA AGATCAAAGT 
GAAGACTGCC TGTACTTAAA CATTTATGTT CCTACAGAAG ACGACATCCA TGATGAGAAT 
GGACTGAGAC CTGTTATGGT GTACATCCAT GGTGGATCCT ACATGGAAGG AACAGGGAAT 
ATGATTGACG GCAGTATTCT AGCCAGCTAT GGAAACGTCA TTGTAGTGAC CCTGAACTAT 
AGGCTGGGAG TGCTGGGGTT CCTGAGTACA GGTGACCAGG CAGCCAAAGG GAATTATGGG 
CTGTTGGACC AGATCCAAGC ACTGCGGTGG ATCAAAGAGA ATATCCAGGC TTTCAAAGGC 
GACCCGAAAA GGGTGACTAT ATTTGGATCA GGAGCTGGGG CGTCCTGTGT TAGCTTGCTG 
ACCCTATCAC ACTATTCAGA AGATTTGTTT CAGAAAGCCA TCATCCAGAG TGGGACTGCT 
CTGTCTAGCT GGGCCGTCAA CTACCAGCCT GCCAAATACA CCCGGATACT AGCTGAGAAA 
GTGGGCTGTA ATATGCTAGA TAGCATAGAC CTGGTGGAAT GTCTACAGAA CAAGAACTAC 
AAGGAGCTGA TTGAACAGTA CATAACACAA GCCAAGTACC ACATTGCGTT TGGTCCAGTA 
ATTGACGGTG ACGTCATCCC AGATGACCCC CAAATTCTGA TGGAGCAAGG GGAGTTCCTC 
AACTATGACA TCATGCTGGG TGTTAACCAG GGTGAGGGTT TTAAGTTTGT GGATGGCATC 
GTGGACAGTG AGGATGGTGT GTCTGCAAAT GACTTTGACT TCGCCGTGTC TGATTTTGTG 
GACCATCTTT ATGGTTATCC AGAGGGCAAG GACACGCTTC GAGAAACCAT CAAGTTCATG 
TATACTGACT GGGCTGACAA GGAGAACCCG GAGACCAGGC GAAAGACTCT AGTTGCACTT 
TTCACTGATC ACCAATGGGT GGCGCCAGCA GTAGCTACAG CAGACCTTCA TGCTCAATAT 
GGATCTCCAA CATATTTTTA TGCTTTTTAC CATCATTGTC AGAGTGAAAT GAAACCTAGC 
TGGTCGGATT CAGCACACGG AGACGAGGTG CCTTATGTGT TTGGAATTCC CATGCTTGGC 
CCCACTGATC TCTTTAATTG TAATTTCTCC AAGAATGATG TCATGCTTTC GGCGGTGGTG 
ATGACGTACT GGACAAATTT CGCTAAAACT GGGGATCCGA ACCAGCCAGT TCCACAGGAC 
ACCAAATTCA TACACACAAA GCCCAACCGC TTTGAGGAAG TAGCCTGGTC GAAATACAAC 
CCCAAAGACC AGCTTTACTT ACACATCGGA CTGAAGCCAA GGGTGAGAGA TCACTATCGC 
GCCACCAAGG TTGCTTTCTG GTTAGAACTG GTACCTCATC TCCATAACAT CAACGAACTC 
TTTCAGTATG TCTCGACCAC TACAAAGATA CCTCCTCAGG ATACTACACC TTTCCCATAC 
ACTAAACGAC TAGGTAAAAC ATGGCCATCC ACCACACGAC ACCCTGGTGT TCCTCCTGCC 
AACACCAAAC AAACCACAGA CCAGCGTAAA GGCAATGATT TGAGTGATGA CTCAGCAGTG 
GTTATCGAGA CAAAGCGCGA TTACTCGACC GAACTCAGTG TGACCATAGC AGTAGGTGCT 
TCTCTCCTTT TCCTCAACAT TCTGGCATTC GCAGCGCTCT ACTACAAGAA GGATAAACGT 
CGACATGAAT CCAACCGACG AGGACCAAGC CCGCAGCGGA ACTCTGCGCC AGCTAATGTG 
GTGGCTAACG CTAATGACAT AGCACATTTG CAGAGTGATG AGCTGATGTC ACTTCAGATG 
AAGCAGCAGC AGATGGAACA CGAGCACCAT CATGAGTGCG ATTCTTTACA AGCTCACGAT 
ACGCTTCGTC TTACCTGCCC GGCTGATTAT ACGCTTACCC TGCGACGTTC ACCTGATGAC 
ATCCCACTCA TGACGCCAAG TACCATAACA ATGATTCCTA ACACTCTGGC CGGAATGCAG 
ACCCTGCACA ATTTCAACAC ATTCGGAGGT AGTCAGAACA GTACCAACTT GCCCCATGGG 
CATTCCACCA CCAGGGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159803.1
CDS	1..2481
Translation	MSRLGGTPWIPLTMVHPLATLRLFTWIVLAAAWLAITRAQQHPIVTTNYGKLRGLKTPLPNEILGPVEQYLGIPYALPPTGERRFQPPEPPMSWPGIRNATQFAPVCPQFLEDRFLLNDMLPVWFTANLDTVVTYVQDQSEDCLYLNIYVPTEDDIHDENGLRPVMVYIHGGSYMEGTGNMIDGSILASYGNVIVVTLNYRLGVLGFLSTGDQAAKGNYGLLDQIQALRWIKENIQAFKGDPKRVTIFGSGAGASCVSLLTLSHYSEDLFQKAIIQSGTALSSWAVNYQPAKYTRILAEKVGCNMLDSIDLVECLQNKNYKELIEQYITQAKYHIAFGPVIDGDVIPDDPQILMEQGEFLNYDIMLGVNQGEGFKFVDGIVDSEDGVSANDFDFAVSDFVDHLYGYPEGKDTLRETIKFMYTDWADKENPETRRKTLVALFTDHQWVAPAVATADLHAQYGSPTYFYAFYHHCQSEMKPSWSDSAHGDEVPYVFGIPMLGPTDLFNCNFSKNDVMLSAVVMTYWTNFAKTGDPNQPVPQDTKFIHTKPNRFEEVAWSKYNPKDQLYLHIGLKPRVRDHYRATKVAFWLELVPHLHNINELFQYVSTTTKIPPQDTTPFPYTKRLGKTWPSTTRHPGVPPANTKQTTDQRKGNDLSDDSAVVIETKRDYSTELSVTIAVGASLLFLNILAFAALYYKKDKRRHESNRRGPSPQRNSAPANVVANANDIAHLQSDELMSLQMKQQQMEHEHHHECDSLQAHDTLRLTCPADYTLTLRRSPDDIPLMTPSTITMIPNTLAGMQTLHNFNTFGGSQNSTNLPHGHSTTRV

Target ORF information:

RefSeq Version	NM_001166331.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neuroligin 4 X-linked a (nlgn4xa), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166331.1

ORF Insert Sequence:

ATGTCACGGC TCGGAGGAAC CCCATGGATA CCACTTACCA TGGTTCATCC GCTGGCTACT 
CTACGCCTTT TTACCTGGAT TGTGCTTGCG GCTGCGTGGC TGGCCATTAC ACGGGCTCAG 
CAGCACCCTA TTGTTACGAC AAACTATGGC AAGCTAAGAG GCTTGAAAAC ACCTCTGCCC 
AATGAGATTC TAGGGCCCGT GGAACAGTAT TTGGGGATCC CCTATGCCCT GCCGCCCACA 
GGAGAAAGAA GGTTTCAGCC CCCAGAGCCT CCAATGTCCT GGCCAGGGAT TCGAAATGCT 
ACACAGTTTG CTCCTGTTTG TCCTCAGTTT CTGGAAGATC GTTTTTTACT CAATGATATG 
TTACCCGTGT GGTTCACCGC CAACTTGGAT ACTGTTGTAA CCTACGTACA AGATCAAAGT 
GAAGACTGCC TGTACTTAAA CATTTATGTT CCTACAGAAG ACGACATCCA TGATGAGAAT 
GGACTGAGAC CTGTTATGGT GTACATCCAT GGTGGATCCT ACATGGAAGG AACAGGGAAT 
ATGATTGACG GCAGTATTCT AGCCAGCTAT GGAAACGTCA TTGTAGTGAC CCTGAACTAT 
AGGCTGGGAG TGCTGGGGTT CCTGAGTACA GGTGACCAGG CAGCCAAAGG GAATTATGGG 
CTGTTGGACC AGATCCAAGC ACTGCGGTGG ATCAAAGAGA ATATCCAGGC TTTCAAAGGC 
GACCCGAAAA GGGTGACTAT ATTTGGATCA GGAGCTGGGG CGTCCTGTGT TAGCTTGCTG 
ACCCTATCAC ACTATTCAGA AGATTTGTTT CAGAAAGCCA TCATCCAGAG TGGGACTGCT 
CTGTCTAGCT GGGCCGTCAA CTACCAGCCT GCCAAATACA CCCGGATACT AGCTGAGAAA 
GTGGGCTGTA ATATGCTAGA TAGCATAGAC CTGGTGGAAT GTCTACAGAA CAAGAACTAC 
AAGGAGCTGA TTGAACAGTA CATAACACAA GCCAAGTACC ACATTGCGTT TGGTCCAGTA 
ATTGACGGTG ACGTCATCCC AGATGACCCC CAAATTCTGA TGGAGCAAGG GGAGTTCCTC 
AACTATGACA TCATGCTGGG TGTTAACCAG GGTGAGGGTT TTAAGTTTGT GGATGGCATC 
GTGGACAGTG AGGATGGTGT GTCTGCAAAT GACTTTGACT TCGCCGTGTC TGATTTTGTG 
GACCATCTTT ATGGTTATCC AGAGGGCAAG GACACGCTTC GAGAAACCAT CAAGTTCATG 
TATACTGACT GGGCTGACAA GGAGAACCCG GAGACCAGGC GAAAGACTCT AGTTGCACTT 
TTCACTGATC ACCAATGGGT GGCGCCAGCA GTAGCTACAG CAGACCTTCA TGCTCAATAT 
GGATCTCCAA CATATTTTTA TGCTTTTTAC CATCATTGTC AGAGTGAAAT GAAACCTAGC 
TGGTCGGATT CAGCACACGG AGACGAGGTG CCTTATGTGT TTGGAATTCC CATGCTTGGC 
CCCACTGATC TCTTTAATTG TAATTTCTCC AAGAATGATG TCATGCTTTC GGCGGTGGTG 
ATGACGTACT GGACAAATTT CGCTAAAACT GGGGATCCGA ACCAGCCAGT TCCACAGGAC 
ACCAAATTCA TACACACAAA GCCCAACCGC TTTGAGGAAG TAGCCTGGTC GAAATACAAC 
CCCAAAGACC AGCTTTACTT ACACATCGGA CTGAAGCCAA GGGTGAGAGA TCACTATCGC 
GCCACCAAGG TTGCTTTCTG GTTAGAACTG GTACCTCATC TCCATAACAT CAACGAACTC 
TTTCAGTATG TCTCGACCAC TACAAAGATA CCTCCTCAGG ATACTACACC TTTCCCATAC 
ACTAAACGAC TAGGTAAAAC ATGGCCATCC ACCACACGAC ACCCTGGTGT TCCTCCTGCC 
AACACCAAAC AAACCACAGA CCAGCGTAAA GGCAATGATT TGAGTGATGA CTCAGCAGTG 
GTTATCGAGA CAAAGCGCGA TTACTCGACC GAACTCAGTG TGACCATAGC AGTAGGTGCT 
TCTCTCCTTT TCCTCAACAT TCTGGCATTC GCAGCGCTCT ACTACAAGAA GGATAAACGT 
CGACATGAAT CCAACCGACG AGGACCAAGC CCGCAGCGGA ACTCTGCGCC AGCTAATGTG 
GTGGCTAACG CTAATGACAT AGCACATTTG CAGAGTGATG AGCTGATGTC ACTTCAGATG 
AAGCAGCAGC AGATGGAACA CGAGCACCAT CATGAGTGCG ATTCTTTACA AGCTCACGAT 
ACGCTTCGTC TTACCTGCCC GGCTGATTAT ACGCTTACCC TGCGACGTTC ACCTGATGAC 
ATCCCACTCA TGACGCCAAG TACCATAACA ATGATTCCTA ACACTCTGGC CGGAATGCAG 
ACCCTGCACA ATTTCAACAC ATTCGGAGGT AGTCAGAACA GTACCAACTT GCCCCATGGG 
CATTCCACCA CCAGGGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nlgn4a ( XM_009292171.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009290446.1 Danio rerio neuroligin 4a (nlgn4a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa43431
Clone ID Related Accession (Same CDS sequence)	XM_009292171.2
Accession Version	XM_009292171.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2532bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	neuroligin-4, X-linked isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292171.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACGGC TCAGAGGAAC CCCATGGATA CCACTTACCA TGGTTCATCC GCTGGCTACT 
CTACGCCTTT TTACCTGGAT TGTGCTTGCG GCTGCGTGGC TGGCCATTAC ATGGGCTCAG 
CAGCACCCTA TTGTTACGAC AAACTATGGC AAGCTAAGAG GCTTGAAAAC ACCTCTGCCC 
AATGAGATTC TAGGGCCCGT GGAACAGTAT TTGGGGATCC CCTATGCCCT GCCGCCCACA 
GGAGAAAGAA GGTTTCAGCC CCCAGAGCCT CCAATGTCCT GGCCAGGGAT TCGAAATGCT 
ACACAGTTTG CTCCTGTTTG TCCTCAGTTT CTGGAAGATC GTTTTTTACT CAATGATATG 
TTACCCGTGT GGTTCACCGC CAACTTGGAT ACTGTTGTAA CCTACGTACA AGATCAAAGT 
GAAGACTGCC TGTACTTAAA CATTTATGTT CCTACAGAAG ACGGAGCCAA CAAAGGCGAT 
GATTTCACCA ATAATGAGGG TGGCGAAAAT AAAGACATCC ATGATGAGAA TGGACTGAGA 
CCTGTTATGG TGTACATCCA TGGTGGATCC TACATGGAAG GAACAGGGAA TATGATTGAC 
GGCAGTATTC TAGCCAGCTA TGGAAACGTC ATTGTAGTGA CCCTGAACTA TAGGCTGGGA 
GTGCTGGGGT TCCTGAGTAC AGGTGACCAG GCAGCCAAAG GGAATTATGG GCTGTTGGAC 
CAGATCCAAG CACTGCGGTG GATCAAAGAG AATATCCAGG CTTTCAAAGG CGACCCGAAA 
AGGGTGACTA TATTTGGATC AGGAGCTGGG GCGTCCTGTG TTAGCTTGCT GACCCTATCA 
CACTATTCAG AAGATTTGTT TCAGAAAGCC ATCATCCAGA GTGGGACTGC TCTGTCTAGC 
TGGGCCGTCA ACTACCAGCC TGCCAAATAC ACCCGGATAC TAGCTGAGAA AGTGGGCTGT 
AATATGCTAG ATAGCATAGA CCTGGTGGAA TGTCTACAGA ACAAGAACTA CAAGGAGCTG 
ATTGAACAGT ACATAACACA AGCCAAGTAC CACATTGCGT TTGGTCCAGT AATTGACGGT 
GACGTCATCC CAGATGACCC CCAAATTCTG ATGGAGCAAG GGGAGTTCCT CAACTATGAC 
ATCATGCTGG GTGTTAACCA GGGTGAGGGT TTTAAGTTTG TGGATGGCAT CGTGGACAGT 
GAGGATGGTG TGTCTGCAAA TGACTTTGAC TTCGCCGTGT CTGATTTTGT GGACCATCTT 
TATGGTTATC CAGAGGGCAA GGACACGCTT CGAGAAACCA TCAAGTTCAT GTATACTGAC 
TGGGCTGACA AGGAGAACCC GGAGACCAGG CGAAAGACTC TAGTTGCACT TTTCACTGAT 
CACCAATGGG TGGCGCCAGC AGTAGCTACA GCAGACCTTC ATGCTCAATA TGGATCTCCA 
ACATATTTTT ATGCTTTTTA CCATCATTGT CAGAGTGAAA TGAAACCTAG CTGGTCGGAT 
TCAGCACACG GAGACGAGGT GCCTTATGTG TTTGGAATTC CCATGCTTGG CCCCACTGAT 
CTCTTTAATT GTAATTTCTC CAAGAATGAT GTCATGCTTT CGGCGGTGGT GATGACGTAC 
TGGACAAATT TCGCTAAAAC TGGGGATCCG AACCAGCCAG TTCCACAGGA CACCAAATTC 
ATACACACAA AGCCCAACCG CTTTGAGGAA GTAGCCTGGT CGAAATACAA CCCCAAAGAC 
CAGCTTTACT TACACATCGG ACTGAAGCCA AGGGTGAGAG ATCACTATCG CGCCACCAAG 
GTTGCTTTCT GGTTAGAACT GGTACCTCAT CTCCATAACA TCAACGAACT CTTTCAGTAT 
GTCTCGACCA CTACAAAGAT ACCTCCTCAG GATACTACAC CTTTCCCATA CACTAAACGA 
CTAGGTAAAA CATGGCCATC CACCACACGA CACCCTGGTG TTCCTCCTGC CAACACCAAA 
CAAACCACAG ACCAGCGTAA AGGCAATGAT TTGAGTGATG ACTCAGCAGT GGTTATCGAG 
ACAAAGCGCG ATTACTCGAC CGAACTCAGT GTGACCATAG CAGTAGGTGC TTCTCTCCTT 
TTCCTCAACA TTCTGGCATT CGCAGCGCTC TACTACAAGA AGGATAAACG TCGACATGAA 
TCCAACCGAC GAGGACCAAG CCCGCAGCGG AACTCTGCGC CAGCTAATGT GGTGGCTAAC 
GCTAATGACA TAGCACATTT GCAGAGTGAT GAGCTGATGT CACTTCAGAT GAAACAGCAG 
CAGATGGAAC ACGAGCACCA TCATGAGTGC GATTCTTTAC AAGCTCACGA TACGCTTCGT 
CTTACCTGCC CGGCTGATTA TACGCTTACC CTGCGACGTT CACCTGATGA CATCCCACTC 
ATGACGCCAA GTACCATAAC AATGATTCCT AACACTCTGG CCGGAATGCA GACCCTGCAC 
AATTTCAACA CATTCGGAGG CAGTCAGAAC AGTACCAACT TGCCCCATGG GCATTCCACC 
ACCAGGGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290446.1
CDS	622..3153
Translation	MSRLRGTPWIPLTMVHPLATLRLFTWIVLAAAWLAITWAQQHPIVTTNYGKLRGLKTPLPNEILGPVEQYLGIPYALPPTGERRFQPPEPPMSWPGIRNATQFAPVCPQFLEDRFLLNDMLPVWFTANLDTVVTYVQDQSEDCLYLNIYVPTEDGANKGDDFTNNEGGENKDIHDENGLRPVMVYIHGGSYMEGTGNMIDGSILASYGNVIVVTLNYRLGVLGFLSTGDQAAKGNYGLLDQIQALRWIKENIQAFKGDPKRVTIFGSGAGASCVSLLTLSHYSEDLFQKAIIQSGTALSSWAVNYQPAKYTRILAEKVGCNMLDSIDLVECLQNKNYKELIEQYITQAKYHIAFGPVIDGDVIPDDPQILMEQGEFLNYDIMLGVNQGEGFKFVDGIVDSEDGVSANDFDFAVSDFVDHLYGYPEGKDTLRETIKFMYTDWADKENPETRRKTLVALFTDHQWVAPAVATADLHAQYGSPTYFYAFYHHCQSEMKPSWSDSAHGDEVPYVFGIPMLGPTDLFNCNFSKNDVMLSAVVMTYWTNFAKTGDPNQPVPQDTKFIHTKPNRFEEVAWSKYNPKDQLYLHIGLKPRVRDHYRATKVAFWLELVPHLHNINELFQYVSTTTKIPPQDTTPFPYTKRLGKTWPSTTRHPGVPPANTKQTTDQRKGNDLSDDSAVVIETKRDYSTELSVTIAVGASLLFLNILAFAALYYKKDKRRHESNRRGPSPQRNSAPANVVANANDIAHLQSDELMSLQMKQQQMEHEHHHECDSLQAHDTLRLTCPADYTLTLRRSPDDIPLMTPSTITMIPNTLAGMQTLHNFNTFGGSQNSTNLPHGHSTTRV

Target ORF information:

RefSeq Version	XM_009292171.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neuroligin 4a (nlgn4a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009292171.2

ORF Insert Sequence:

ATGTCACGGC TCAGAGGAAC CCCATGGATA CCACTTACCA TGGTTCATCC GCTGGCTACT 
CTACGCCTTT TTACCTGGAT TGTGCTTGCG GCTGCGTGGC TGGCCATTAC ATGGGCTCAG 
CAGCACCCTA TTGTTACGAC AAACTATGGC AAGCTAAGAG GCTTGAAAAC ACCTCTGCCC 
AATGAGATTC TAGGGCCCGT GGAACAGTAT TTGGGGATCC CCTATGCCCT GCCGCCCACA 
GGAGAAAGAA GGTTTCAGCC CCCAGAGCCT CCAATGTCCT GGCCAGGGAT TCGAAATGCT 
ACACAGTTTG CTCCTGTTTG TCCTCAGTTT CTGGAAGATC GTTTTTTACT CAATGATATG 
TTACCCGTGT GGTTCACCGC CAACTTGGAT ACTGTTGTAA CCTACGTACA AGATCAAAGT 
GAAGACTGCC TGTACTTAAA CATTTATGTT CCTACAGAAG ACGGAGCCAA CAAAGGCGAT 
GATTTCACCA ATAATGAGGG TGGCGAAAAT AAAGACATCC ATGATGAGAA TGGACTGAGA 
CCTGTTATGG TGTACATCCA TGGTGGATCC TACATGGAAG GAACAGGGAA TATGATTGAC 
GGCAGTATTC TAGCCAGCTA TGGAAACGTC ATTGTAGTGA CCCTGAACTA TAGGCTGGGA 
GTGCTGGGGT TCCTGAGTAC AGGTGACCAG GCAGCCAAAG GGAATTATGG GCTGTTGGAC 
CAGATCCAAG CACTGCGGTG GATCAAAGAG AATATCCAGG CTTTCAAAGG CGACCCGAAA 
AGGGTGACTA TATTTGGATC AGGAGCTGGG GCGTCCTGTG TTAGCTTGCT GACCCTATCA 
CACTATTCAG AAGATTTGTT TCAGAAAGCC ATCATCCAGA GTGGGACTGC TCTGTCTAGC 
TGGGCCGTCA ACTACCAGCC TGCCAAATAC ACCCGGATAC TAGCTGAGAA AGTGGGCTGT 
AATATGCTAG ATAGCATAGA CCTGGTGGAA TGTCTACAGA ACAAGAACTA CAAGGAGCTG 
ATTGAACAGT ACATAACACA AGCCAAGTAC CACATTGCGT TTGGTCCAGT AATTGACGGT 
GACGTCATCC CAGATGACCC CCAAATTCTG ATGGAGCAAG GGGAGTTCCT CAACTATGAC 
ATCATGCTGG GTGTTAACCA GGGTGAGGGT TTTAAGTTTG TGGATGGCAT CGTGGACAGT 
GAGGATGGTG TGTCTGCAAA TGACTTTGAC TTCGCCGTGT CTGATTTTGT GGACCATCTT 
TATGGTTATC CAGAGGGCAA GGACACGCTT CGAGAAACCA TCAAGTTCAT GTATACTGAC 
TGGGCTGACA AGGAGAACCC GGAGACCAGG CGAAAGACTC TAGTTGCACT TTTCACTGAT 
CACCAATGGG TGGCGCCAGC AGTAGCTACA GCAGACCTTC ATGCTCAATA TGGATCTCCA 
ACATATTTTT ATGCTTTTTA CCATCATTGT CAGAGTGAAA TGAAACCTAG CTGGTCGGAT 
TCAGCACACG GAGACGAGGT GCCTTATGTG TTTGGAATTC CCATGCTTGG CCCCACTGAT 
CTCTTTAATT GTAATTTCTC CAAGAATGAT GTCATGCTTT CGGCGGTGGT GATGACGTAC 
TGGACAAATT TCGCTAAAAC TGGGGATCCG AACCAGCCAG TTCCACAGGA CACCAAATTC 
ATACACACAA AGCCCAACCG CTTTGAGGAA GTAGCCTGGT CGAAATACAA CCCCAAAGAC 
CAGCTTTACT TACACATCGG ACTGAAGCCA AGGGTGAGAG ATCACTATCG CGCCACCAAG 
GTTGCTTTCT GGTTAGAACT GGTACCTCAT CTCCATAACA TCAACGAACT CTTTCAGTAT 
GTCTCGACCA CTACAAAGAT ACCTCCTCAG GATACTACAC CTTTCCCATA CACTAAACGA 
CTAGGTAAAA CATGGCCATC CACCACACGA CACCCTGGTG TTCCTCCTGC CAACACCAAA 
CAAACCACAG ACCAGCGTAA AGGCAATGAT TTGAGTGATG ACTCAGCAGT GGTTATCGAG 
ACAAAGCGCG ATTACTCGAC CGAACTCAGT GTGACCATAG CAGTAGGTGC TTCTCTCCTT 
TTCCTCAACA TTCTGGCATT CGCAGCGCTC TACTACAAGA AGGATAAACG TCGACATGAA 
TCCAACCGAC GAGGACCAAG CCCGCAGCGG AACTCTGCGC CAGCTAATGT GGTGGCTAAC 
GCTAATGACA TAGCACATTT GCAGAGTGAT GAGCTGATGT CACTTCAGAT GAAACAGCAG 
CAGATGGAAC ACGAGCACCA TCATGAGTGC GATTCTTTAC AAGCTCACGA TACGCTTCGT 
CTTACCTGCC CGGCTGATTA TACGCTTACC CTGCGACGTT CACCTGATGA CATCCCACTC 
ATGACGCCAA GTACCATAAC AATGATTCCT AACACTCTGG CCGGAATGCA GACCCTGCAC 
AATTTCAACA CATTCGGAGG CAGTCAGAAC AGTACCAACT TGCCCCATGG GCATTCCACC 
ACCAGGGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nlgn4a ( XM_009292174.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009290449.1 Danio rerio neuroligin 4a (nlgn4a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa43432
Clone ID Related Accession (Same CDS sequence)	XM_009292174.2 , XM_009292174.3
Accession Version	XM_009292174.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2481bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	neuroligin-4, X-linked isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292174.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACGGC TCAGAGGAAC CCCATGGATA CCACTTACCA TGGTTCATCC GCTGGCTACT 
CTACGCCTTT TTACCTGGAT TGTGCTTGCG GCTGCGTGGC TGGCCATTAC ATGGGCTCAG 
CAGCACCCTA TTGTTACGAC AAACTATGGC AAGCTAAGAG GCTTGAAAAC ACCTCTGCCC 
AATGAGATTC TAGGGCCCGT GGAACAGTAT TTGGGGATCC CCTATGCCCT GCCGCCCACA 
GGAGAAAGAA GGTTTCAGCC CCCAGAGCCT CCAATGTCCT GGCCAGGGAT TCGAAATGCT 
ACACAGTTTG CTCCTGTTTG TCCTCAGTTT CTGGAAGATC GTTTTTTACT CAATGATATG 
TTACCCGTGT GGTTCACCGC CAACTTGGAT ACTGTTGTAA CCTACGTACA AGATCAAAGT 
GAAGACTGCC TGTACTTAAA CATTTATGTT CCTACAGAAG ACGACATCCA TGATGAGAAT 
GGACTGAGAC CTGTTATGGT GTACATCCAT GGTGGATCCT ACATGGAAGG AACAGGGAAT 
ATGATTGACG GCAGTATTCT AGCCAGCTAT GGAAACGTCA TTGTAGTGAC CCTGAACTAT 
AGGCTGGGAG TGCTGGGGTT CCTGAGTACA GGTGACCAGG CAGCCAAAGG GAATTATGGG 
CTGTTGGACC AGATCCAAGC ACTGCGGTGG ATCAAAGAGA ATATCCAGGC TTTCAAAGGC 
GACCCGAAAA GGGTGACTAT ATTTGGATCA GGAGCTGGGG CGTCCTGTGT TAGCTTGCTG 
ACCCTATCAC ACTATTCAGA AGATTTGTTT CAGAAAGCCA TCATCCAGAG TGGGACTGCT 
CTGTCTAGCT GGGCCGTCAA CTACCAGCCT GCCAAATACA CCCGGATACT AGCTGAGAAA 
GTGGGCTGTA ATATGCTAGA TAGCATAGAC CTGGTGGAAT GTCTACAGAA CAAGAACTAC 
AAGGAGCTGA TTGAACAGTA CATAACACAA GCCAAGTACC ACATTGCGTT TGGTCCAGTA 
ATTGACGGTG ACGTCATCCC AGATGACCCC CAAATTCTGA TGGAGCAAGG GGAGTTCCTC 
AACTATGACA TCATGCTGGG TGTTAACCAG GGTGAGGGTT TTAAGTTTGT GGATGGCATC 
GTGGACAGTG AGGATGGTGT GTCTGCAAAT GACTTTGACT TCGCCGTGTC TGATTTTGTG 
GACCATCTTT ATGGTTATCC AGAGGGCAAG GACACGCTTC GAGAAACCAT CAAGTTCATG 
TATACTGACT GGGCTGACAA GGAGAACCCG GAGACCAGGC GAAAGACTCT AGTTGCACTT 
TTCACTGATC ACCAATGGGT GGCGCCAGCA GTAGCTACAG CAGACCTTCA TGCTCAATAT 
GGATCTCCAA CATATTTTTA TGCTTTTTAC CATCATTGTC AGAGTGAAAT GAAACCTAGC 
TGGTCGGATT CAGCACACGG AGACGAGGTG CCTTATGTGT TTGGAATTCC CATGCTTGGC 
CCCACTGATC TCTTTAATTG TAATTTCTCC AAGAATGATG TCATGCTTTC GGCGGTGGTG 
ATGACGTACT GGACAAATTT CGCTAAAACT GGGGATCCGA ACCAGCCAGT TCCACAGGAC 
ACCAAATTCA TACACACAAA GCCCAACCGC TTTGAGGAAG TAGCCTGGTC GAAATACAAC 
CCCAAAGACC AGCTTTACTT ACACATCGGA CTGAAGCCAA GGGTGAGAGA TCACTATCGC 
GCCACCAAGG TTGCTTTCTG GTTAGAACTG GTACCTCATC TCCATAACAT CAACGAACTC 
TTTCAGTATG TCTCGACCAC TACAAAGATA CCTCCTCAGG ATACTACACC TTTCCCATAC 
ACTAAACGAC TAGGTAAAAC ATGGCCATCC ACCACACGAC ACCCTGGTGT TCCTCCTGCC 
AACACCAAAC AAACCACAGA CCAGCGTAAA GGCAATGATT TGAGTGATGA CTCAGCAGTG 
GTTATCGAGA CAAAGCGCGA TTACTCGACC GAACTCAGTG TGACCATAGC AGTAGGTGCT 
TCTCTCCTTT TCCTCAACAT TCTGGCATTC GCAGCGCTCT ACTACAAGAA GGATAAACGT 
CGACATGAAT CCAACCGACG AGGACCAAGC CCGCAGCGGA ACTCTGCGCC AGCTAATGTG 
GTGGCTAACG CTAATGACAT AGCACATTTG CAGAGTGATG AGCTGATGTC ACTTCAGATG 
AAACAGCAGC AGATGGAACA CGAGCACCAT CATGAGTGCG ATTCTTTACA AGCTCACGAT 
ACGCTTCGTC TTACCTGCCC GGCTGATTAT ACGCTTACCC TGCGACGTTC ACCTGATGAC 
ATCCCACTCA TGACGCCAAG TACCATAACA ATGATTCCTA ACACTCTGGC CGGAATGCAG 
ACCCTGCACA ATTTCAACAC ATTCGGAGGC AGTCAGAACA GTACCAACTT GCCCCATGGG 
CATTCCACCA CCAGGGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290449.1
CDS	622..3102
Translation	MSRLRGTPWIPLTMVHPLATLRLFTWIVLAAAWLAITWAQQHPIVTTNYGKLRGLKTPLPNEILGPVEQYLGIPYALPPTGERRFQPPEPPMSWPGIRNATQFAPVCPQFLEDRFLLNDMLPVWFTANLDTVVTYVQDQSEDCLYLNIYVPTEDDIHDENGLRPVMVYIHGGSYMEGTGNMIDGSILASYGNVIVVTLNYRLGVLGFLSTGDQAAKGNYGLLDQIQALRWIKENIQAFKGDPKRVTIFGSGAGASCVSLLTLSHYSEDLFQKAIIQSGTALSSWAVNYQPAKYTRILAEKVGCNMLDSIDLVECLQNKNYKELIEQYITQAKYHIAFGPVIDGDVIPDDPQILMEQGEFLNYDIMLGVNQGEGFKFVDGIVDSEDGVSANDFDFAVSDFVDHLYGYPEGKDTLRETIKFMYTDWADKENPETRRKTLVALFTDHQWVAPAVATADLHAQYGSPTYFYAFYHHCQSEMKPSWSDSAHGDEVPYVFGIPMLGPTDLFNCNFSKNDVMLSAVVMTYWTNFAKTGDPNQPVPQDTKFIHTKPNRFEEVAWSKYNPKDQLYLHIGLKPRVRDHYRATKVAFWLELVPHLHNINELFQYVSTTTKIPPQDTTPFPYTKRLGKTWPSTTRHPGVPPANTKQTTDQRKGNDLSDDSAVVIETKRDYSTELSVTIAVGASLLFLNILAFAALYYKKDKRRHESNRRGPSPQRNSAPANVVANANDIAHLQSDELMSLQMKQQQMEHEHHHECDSLQAHDTLRLTCPADYTLTLRRSPDDIPLMTPSTITMIPNTLAGMQTLHNFNTFGGSQNSTNLPHGHSTTRV

Target ORF information:

RefSeq Version	XM_009292174.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neuroligin 4a (nlgn4a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009292174.2

ORF Insert Sequence:

ATGTCACGGC TCAGAGGAAC CCCATGGATA CCACTTACCA TGGTTCATCC GCTGGCTACT 
CTACGCCTTT TTACCTGGAT TGTGCTTGCG GCTGCGTGGC TGGCCATTAC ATGGGCTCAG 
CAGCACCCTA TTGTTACGAC AAACTATGGC AAGCTAAGAG GCTTGAAAAC ACCTCTGCCC 
AATGAGATTC TAGGGCCCGT GGAACAGTAT TTGGGGATCC CCTATGCCCT GCCGCCCACA 
GGAGAAAGAA GGTTTCAGCC CCCAGAGCCT CCAATGTCCT GGCCAGGGAT TCGAAATGCT 
ACACAGTTTG CTCCTGTTTG TCCTCAGTTT CTGGAAGATC GTTTTTTACT CAATGATATG 
TTACCCGTGT GGTTCACCGC CAACTTGGAT ACTGTTGTAA CCTACGTACA AGATCAAAGT 
GAAGACTGCC TGTACTTAAA CATTTATGTT CCTACAGAAG ACGACATCCA TGATGAGAAT 
GGACTGAGAC CTGTTATGGT GTACATCCAT GGTGGATCCT ACATGGAAGG AACAGGGAAT 
ATGATTGACG GCAGTATTCT AGCCAGCTAT GGAAACGTCA TTGTAGTGAC CCTGAACTAT 
AGGCTGGGAG TGCTGGGGTT CCTGAGTACA GGTGACCAGG CAGCCAAAGG GAATTATGGG 
CTGTTGGACC AGATCCAAGC ACTGCGGTGG ATCAAAGAGA ATATCCAGGC TTTCAAAGGC 
GACCCGAAAA GGGTGACTAT ATTTGGATCA GGAGCTGGGG CGTCCTGTGT TAGCTTGCTG 
ACCCTATCAC ACTATTCAGA AGATTTGTTT CAGAAAGCCA TCATCCAGAG TGGGACTGCT 
CTGTCTAGCT GGGCCGTCAA CTACCAGCCT GCCAAATACA CCCGGATACT AGCTGAGAAA 
GTGGGCTGTA ATATGCTAGA TAGCATAGAC CTGGTGGAAT GTCTACAGAA CAAGAACTAC 
AAGGAGCTGA TTGAACAGTA CATAACACAA GCCAAGTACC ACATTGCGTT TGGTCCAGTA 
ATTGACGGTG ACGTCATCCC AGATGACCCC CAAATTCTGA TGGAGCAAGG GGAGTTCCTC 
AACTATGACA TCATGCTGGG TGTTAACCAG GGTGAGGGTT TTAAGTTTGT GGATGGCATC 
GTGGACAGTG AGGATGGTGT GTCTGCAAAT GACTTTGACT TCGCCGTGTC TGATTTTGTG 
GACCATCTTT ATGGTTATCC AGAGGGCAAG GACACGCTTC GAGAAACCAT CAAGTTCATG 
TATACTGACT GGGCTGACAA GGAGAACCCG GAGACCAGGC GAAAGACTCT AGTTGCACTT 
TTCACTGATC ACCAATGGGT GGCGCCAGCA GTAGCTACAG CAGACCTTCA TGCTCAATAT 
GGATCTCCAA CATATTTTTA TGCTTTTTAC CATCATTGTC AGAGTGAAAT GAAACCTAGC 
TGGTCGGATT CAGCACACGG AGACGAGGTG CCTTATGTGT TTGGAATTCC CATGCTTGGC 
CCCACTGATC TCTTTAATTG TAATTTCTCC AAGAATGATG TCATGCTTTC GGCGGTGGTG 
ATGACGTACT GGACAAATTT CGCTAAAACT GGGGATCCGA ACCAGCCAGT TCCACAGGAC 
ACCAAATTCA TACACACAAA GCCCAACCGC TTTGAGGAAG TAGCCTGGTC GAAATACAAC 
CCCAAAGACC AGCTTTACTT ACACATCGGA CTGAAGCCAA GGGTGAGAGA TCACTATCGC 
GCCACCAAGG TTGCTTTCTG GTTAGAACTG GTACCTCATC TCCATAACAT CAACGAACTC 
TTTCAGTATG TCTCGACCAC TACAAAGATA CCTCCTCAGG ATACTACACC TTTCCCATAC 
ACTAAACGAC TAGGTAAAAC ATGGCCATCC ACCACACGAC ACCCTGGTGT TCCTCCTGCC 
AACACCAAAC AAACCACAGA CCAGCGTAAA GGCAATGATT TGAGTGATGA CTCAGCAGTG 
GTTATCGAGA CAAAGCGCGA TTACTCGACC GAACTCAGTG TGACCATAGC AGTAGGTGCT 
TCTCTCCTTT TCCTCAACAT TCTGGCATTC GCAGCGCTCT ACTACAAGAA GGATAAACGT 
CGACATGAAT CCAACCGACG AGGACCAAGC CCGCAGCGGA ACTCTGCGCC AGCTAATGTG 
GTGGCTAACG CTAATGACAT AGCACATTTG CAGAGTGATG AGCTGATGTC ACTTCAGATG 
AAACAGCAGC AGATGGAACA CGAGCACCAT CATGAGTGCG ATTCTTTACA AGCTCACGAT 
ACGCTTCGTC TTACCTGCCC GGCTGATTAT ACGCTTACCC TGCGACGTTC ACCTGATGAC 
ATCCCACTCA TGACGCCAAG TACCATAACA ATGATTCCTA ACACTCTGGC CGGAATGCAG 
ACCCTGCACA ATTTCAACAC ATTCGGAGGC AGTCAGAACA GTACCAACTT GCCCCATGGG 
CATTCCACCA CCAGGGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nlgn4a ( XM_009292174.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009290449.1 Danio rerio neuroligin 4a (nlgn4a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa43432
Clone ID Related Accession (Same CDS sequence)	XM_009292174.2 , XM_009292174.3
Accession Version	XM_009292174.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2481bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neuroligin-4, X-linked isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009292174.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACGGC TCAGAGGAAC CCCATGGATA CCACTTACCA TGGTTCATCC GCTGGCTACT 
CTACGCCTTT TTACCTGGAT TGTGCTTGCG GCTGCGTGGC TGGCCATTAC ATGGGCTCAG 
CAGCACCCTA TTGTTACGAC AAACTATGGC AAGCTAAGAG GCTTGAAAAC ACCTCTGCCC 
AATGAGATTC TAGGGCCCGT GGAACAGTAT TTGGGGATCC CCTATGCCCT GCCGCCCACA 
GGAGAAAGAA GGTTTCAGCC CCCAGAGCCT CCAATGTCCT GGCCAGGGAT TCGAAATGCT 
ACACAGTTTG CTCCTGTTTG TCCTCAGTTT CTGGAAGATC GTTTTTTACT CAATGATATG 
TTACCCGTGT GGTTCACCGC CAACTTGGAT ACTGTTGTAA CCTACGTACA AGATCAAAGT 
GAAGACTGCC TGTACTTAAA CATTTATGTT CCTACAGAAG ACGACATCCA TGATGAGAAT 
GGACTGAGAC CTGTTATGGT GTACATCCAT GGTGGATCCT ACATGGAAGG AACAGGGAAT 
ATGATTGACG GCAGTATTCT AGCCAGCTAT GGAAACGTCA TTGTAGTGAC CCTGAACTAT 
AGGCTGGGAG TGCTGGGGTT CCTGAGTACA GGTGACCAGG CAGCCAAAGG GAATTATGGG 
CTGTTGGACC AGATCCAAGC ACTGCGGTGG ATCAAAGAGA ATATCCAGGC TTTCAAAGGC 
GACCCGAAAA GGGTGACTAT ATTTGGATCA GGAGCTGGGG CGTCCTGTGT TAGCTTGCTG 
ACCCTATCAC ACTATTCAGA AGATTTGTTT CAGAAAGCCA TCATCCAGAG TGGGACTGCT 
CTGTCTAGCT GGGCCGTCAA CTACCAGCCT GCCAAATACA CCCGGATACT AGCTGAGAAA 
GTGGGCTGTA ATATGCTAGA TAGCATAGAC CTGGTGGAAT GTCTACAGAA CAAGAACTAC 
AAGGAGCTGA TTGAACAGTA CATAACACAA GCCAAGTACC ACATTGCGTT TGGTCCAGTA 
ATTGACGGTG ACGTCATCCC AGATGACCCC CAAATTCTGA TGGAGCAAGG GGAGTTCCTC 
AACTATGACA TCATGCTGGG TGTTAACCAG GGTGAGGGTT TTAAGTTTGT GGATGGCATC 
GTGGACAGTG AGGATGGTGT GTCTGCAAAT GACTTTGACT TCGCCGTGTC TGATTTTGTG 
GACCATCTTT ATGGTTATCC AGAGGGCAAG GACACGCTTC GAGAAACCAT CAAGTTCATG 
TATACTGACT GGGCTGACAA GGAGAACCCG GAGACCAGGC GAAAGACTCT AGTTGCACTT 
TTCACTGATC ACCAATGGGT GGCGCCAGCA GTAGCTACAG CAGACCTTCA TGCTCAATAT 
GGATCTCCAA CATATTTTTA TGCTTTTTAC CATCATTGTC AGAGTGAAAT GAAACCTAGC 
TGGTCGGATT CAGCACACGG AGACGAGGTG CCTTATGTGT TTGGAATTCC CATGCTTGGC 
CCCACTGATC TCTTTAATTG TAATTTCTCC AAGAATGATG TCATGCTTTC GGCGGTGGTG 
ATGACGTACT GGACAAATTT CGCTAAAACT GGGGATCCGA ACCAGCCAGT TCCACAGGAC 
ACCAAATTCA TACACACAAA GCCCAACCGC TTTGAGGAAG TAGCCTGGTC GAAATACAAC 
CCCAAAGACC AGCTTTACTT ACACATCGGA CTGAAGCCAA GGGTGAGAGA TCACTATCGC 
GCCACCAAGG TTGCTTTCTG GTTAGAACTG GTACCTCATC TCCATAACAT CAACGAACTC 
TTTCAGTATG TCTCGACCAC TACAAAGATA CCTCCTCAGG ATACTACACC TTTCCCATAC 
ACTAAACGAC TAGGTAAAAC ATGGCCATCC ACCACACGAC ACCCTGGTGT TCCTCCTGCC 
AACACCAAAC AAACCACAGA CCAGCGTAAA GGCAATGATT TGAGTGATGA CTCAGCAGTG 
GTTATCGAGA CAAAGCGCGA TTACTCGACC GAACTCAGTG TGACCATAGC AGTAGGTGCT 
TCTCTCCTTT TCCTCAACAT TCTGGCATTC GCAGCGCTCT ACTACAAGAA GGATAAACGT 
CGACATGAAT CCAACCGACG AGGACCAAGC CCGCAGCGGA ACTCTGCGCC AGCTAATGTG 
GTGGCTAACG CTAATGACAT AGCACATTTG CAGAGTGATG AGCTGATGTC ACTTCAGATG 
AAACAGCAGC AGATGGAACA CGAGCACCAT CATGAGTGCG ATTCTTTACA AGCTCACGAT 
ACGCTTCGTC TTACCTGCCC GGCTGATTAT ACGCTTACCC TGCGACGTTC ACCTGATGAC 
ATCCCACTCA TGACGCCAAG TACCATAACA ATGATTCCTA ACACTCTGGC CGGAATGCAG 
ACCCTGCACA ATTTCAACAC ATTCGGAGGC AGTCAGAACA GTACCAACTT GCCCCATGGG 
CATTCCACCA CCAGGGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290449.1
CDS	627..3107
Translation	MSRLRGTPWIPLTMVHPLATLRLFTWIVLAAAWLAITWAQQHPIVTTNYGKLRGLKTPLPNEILGPVEQYLGIPYALPPTGERRFQPPEPPMSWPGIRNATQFAPVCPQFLEDRFLLNDMLPVWFTANLDTVVTYVQDQSEDCLYLNIYVPTEDDIHDENGLRPVMVYIHGGSYMEGTGNMIDGSILASYGNVIVVTLNYRLGVLGFLSTGDQAAKGNYGLLDQIQALRWIKENIQAFKGDPKRVTIFGSGAGASCVSLLTLSHYSEDLFQKAIIQSGTALSSWAVNYQPAKYTRILAEKVGCNMLDSIDLVECLQNKNYKELIEQYITQAKYHIAFGPVIDGDVIPDDPQILMEQGEFLNYDIMLGVNQGEGFKFVDGIVDSEDGVSANDFDFAVSDFVDHLYGYPEGKDTLRETIKFMYTDWADKENPETRRKTLVALFTDHQWVAPAVATADLHAQYGSPTYFYAFYHHCQSEMKPSWSDSAHGDEVPYVFGIPMLGPTDLFNCNFSKNDVMLSAVVMTYWTNFAKTGDPNQPVPQDTKFIHTKPNRFEEVAWSKYNPKDQLYLHIGLKPRVRDHYRATKVAFWLELVPHLHNINELFQYVSTTTKIPPQDTTPFPYTKRLGKTWPSTTRHPGVPPANTKQTTDQRKGNDLSDDSAVVIETKRDYSTELSVTIAVGASLLFLNILAFAALYYKKDKRRHESNRRGPSPQRNSAPANVVANANDIAHLQSDELMSLQMKQQQMEHEHHHECDSLQAHDTLRLTCPADYTLTLRRSPDDIPLMTPSTITMIPNTLAGMQTLHNFNTFGGSQNSTNLPHGHSTTRV

Target ORF information:

RefSeq Version	XM_009292174.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neuroligin 4a (nlgn4a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009292174.3

ORF Insert Sequence:

ATGTCACGGC TCAGAGGAAC CCCATGGATA CCACTTACCA TGGTTCATCC GCTGGCTACT 
CTACGCCTTT TTACCTGGAT TGTGCTTGCG GCTGCGTGGC TGGCCATTAC ATGGGCTCAG 
CAGCACCCTA TTGTTACGAC AAACTATGGC AAGCTAAGAG GCTTGAAAAC ACCTCTGCCC 
AATGAGATTC TAGGGCCCGT GGAACAGTAT TTGGGGATCC CCTATGCCCT GCCGCCCACA 
GGAGAAAGAA GGTTTCAGCC CCCAGAGCCT CCAATGTCCT GGCCAGGGAT TCGAAATGCT 
ACACAGTTTG CTCCTGTTTG TCCTCAGTTT CTGGAAGATC GTTTTTTACT CAATGATATG 
TTACCCGTGT GGTTCACCGC CAACTTGGAT ACTGTTGTAA CCTACGTACA AGATCAAAGT 
GAAGACTGCC TGTACTTAAA CATTTATGTT CCTACAGAAG ACGACATCCA TGATGAGAAT 
GGACTGAGAC CTGTTATGGT GTACATCCAT GGTGGATCCT ACATGGAAGG AACAGGGAAT 
ATGATTGACG GCAGTATTCT AGCCAGCTAT GGAAACGTCA TTGTAGTGAC CCTGAACTAT 
AGGCTGGGAG TGCTGGGGTT CCTGAGTACA GGTGACCAGG CAGCCAAAGG GAATTATGGG 
CTGTTGGACC AGATCCAAGC ACTGCGGTGG ATCAAAGAGA ATATCCAGGC TTTCAAAGGC 
GACCCGAAAA GGGTGACTAT ATTTGGATCA GGAGCTGGGG CGTCCTGTGT TAGCTTGCTG 
ACCCTATCAC ACTATTCAGA AGATTTGTTT CAGAAAGCCA TCATCCAGAG TGGGACTGCT 
CTGTCTAGCT GGGCCGTCAA CTACCAGCCT GCCAAATACA CCCGGATACT AGCTGAGAAA 
GTGGGCTGTA ATATGCTAGA TAGCATAGAC CTGGTGGAAT GTCTACAGAA CAAGAACTAC 
AAGGAGCTGA TTGAACAGTA CATAACACAA GCCAAGTACC ACATTGCGTT TGGTCCAGTA 
ATTGACGGTG ACGTCATCCC AGATGACCCC CAAATTCTGA TGGAGCAAGG GGAGTTCCTC 
AACTATGACA TCATGCTGGG TGTTAACCAG GGTGAGGGTT TTAAGTTTGT GGATGGCATC 
GTGGACAGTG AGGATGGTGT GTCTGCAAAT GACTTTGACT TCGCCGTGTC TGATTTTGTG 
GACCATCTTT ATGGTTATCC AGAGGGCAAG GACACGCTTC GAGAAACCAT CAAGTTCATG 
TATACTGACT GGGCTGACAA GGAGAACCCG GAGACCAGGC GAAAGACTCT AGTTGCACTT 
TTCACTGATC ACCAATGGGT GGCGCCAGCA GTAGCTACAG CAGACCTTCA TGCTCAATAT 
GGATCTCCAA CATATTTTTA TGCTTTTTAC CATCATTGTC AGAGTGAAAT GAAACCTAGC 
TGGTCGGATT CAGCACACGG AGACGAGGTG CCTTATGTGT TTGGAATTCC CATGCTTGGC 
CCCACTGATC TCTTTAATTG TAATTTCTCC AAGAATGATG TCATGCTTTC GGCGGTGGTG 
ATGACGTACT GGACAAATTT CGCTAAAACT GGGGATCCGA ACCAGCCAGT TCCACAGGAC 
ACCAAATTCA TACACACAAA GCCCAACCGC TTTGAGGAAG TAGCCTGGTC GAAATACAAC 
CCCAAAGACC AGCTTTACTT ACACATCGGA CTGAAGCCAA GGGTGAGAGA TCACTATCGC 
GCCACCAAGG TTGCTTTCTG GTTAGAACTG GTACCTCATC TCCATAACAT CAACGAACTC 
TTTCAGTATG TCTCGACCAC TACAAAGATA CCTCCTCAGG ATACTACACC TTTCCCATAC 
ACTAAACGAC TAGGTAAAAC ATGGCCATCC ACCACACGAC ACCCTGGTGT TCCTCCTGCC 
AACACCAAAC AAACCACAGA CCAGCGTAAA GGCAATGATT TGAGTGATGA CTCAGCAGTG 
GTTATCGAGA CAAAGCGCGA TTACTCGACC GAACTCAGTG TGACCATAGC AGTAGGTGCT 
TCTCTCCTTT TCCTCAACAT TCTGGCATTC GCAGCGCTCT ACTACAAGAA GGATAAACGT 
CGACATGAAT CCAACCGACG AGGACCAAGC CCGCAGCGGA ACTCTGCGCC AGCTAATGTG 
GTGGCTAACG CTAATGACAT AGCACATTTG CAGAGTGATG AGCTGATGTC ACTTCAGATG 
AAACAGCAGC AGATGGAACA CGAGCACCAT CATGAGTGCG ATTCTTTACA AGCTCACGAT 
ACGCTTCGTC TTACCTGCCC GGCTGATTAT ACGCTTACCC TGCGACGTTC ACCTGATGAC 
ATCCCACTCA TGACGCCAAG TACCATAACA ATGATTCCTA ACACTCTGGC CGGAATGCAG 
ACCCTGCACA ATTTCAACAC ATTCGGAGGC AGTCAGAACA GTACCAACTT GCCCCATGGG 
CATTCCACCA CCAGGGTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nlgn4a ( XM_021475240.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330915.1 Danio rerio neuroligin 4a (nlgn4a), transcript variant X1, mRNA.

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CloneID	ODa43430
Clone ID Related Accession (Same CDS sequence)	XM_021475240.1
Accession Version	XM_021475240.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	neuroligin-4, X-linked isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACGGC TCAGAGGAAC CCCATGGATA CCACTTACCA TGGTTCATCC GCTGGCTACT 
CTACGCCTTT TTACCTGGAT TGTGCTTGCG GCTGCGTGGC TGGCCATTAC ATGGGCTCAG 
CAGCACCCTA TTGTTACGAC AAACTATGGC AAGCTAAGAG GCTTGAAAAC ACCTCTGCCC 
AATGAGATTC TAGGGCCCGT GGAACAGTAT TTGGGGATCC CCTATGCCCT GCCGCCCACA 
GGAGAAAGAA GGTTTCAGCC CCCAGAGCCT CCAATGTCCT GGCCAGGGAT TCGAAATGCT 
ACACAGTTTG CTCCTGTTTG TCCTCAGTTT CTGGAAGATC GTTTTTTACT CAATGATATG 
TTACCCGTGT GGTTCACCGC CAACTTGGAT ACTGTTGTAA CCTACGTACA AGATCAAAGT 
GAAGACTGCC TGTACTTAAA CATTTATGTT CCTACAGAAG ACGTAGGAGC CAACAAAGGC 
GATGATTTCA CCAATAATGA GGGTGGCGAA AATAAAGACA TCCATGATGA GAATGGACTG 
AGACCTGTTA TGGTGTACAT CCATGGTGGA TCCTACATGG AAGGAACAGG GAATATGATT 
GACGGCAGTA TTCTAGCCAG CTATGGAAAC GTCATTGTAG TGACCCTGAA CTATAGGCTG 
GGAGTGCTGG GGTTCCTGAG TACAGGTGAC CAGGCAGCCA AAGGGAATTA TGGGCTGTTG 
GACCAGATCC AAGCACTGCG GTGGATCAAA GAGAATATCC AGGCTTTCAA AGGCGACCCG 
AAAAGGGTGA CTATATTTGG ATCAGGAGCT GGGGCGTCCT GTGTTAGCTT GCTGACCCTA 
TCACACTATT CAGAAGATTT GTTTCAGAAA GCCATCATCC AGAGTGGGAC TGCTCTGTCT 
AGCTGGGCCG TCAACTACCA GCCTGCCAAA TACACCCGGA TACTAGCTGA GAAAGTGGGC 
TGTAATATGC TAGATAGCAT AGACCTGGTG GAATGTCTAC AGAACAAGAA CTACAAGGAG 
CTGATTGAAC AGTACATAAC ACAAGCCAAG TACCACATTG CGTTTGGTCC AGTAATTGAC 
GGTGACGTCA TCCCAGATGA CCCCCAAATT CTGATGGAGC AAGGGGAGTT CCTCAACTAT 
GACATCATGC TGGGTGTTAA CCAGGGTGAG GGTTTTAAGT TTGTGGATGG CATCGTGGAC 
AGTGAGGATG GTGTGTCTGC AAATGACTTT GACTTCGCCG TGTCTGATTT TGTGGACCAT 
CTTTATGGTT ATCCAGAGGG CAAGGACACG CTTCGAGAAA CCATCAAGTT CATGTATACT 
GACTGGGCTG ACAAGGAGAA CCCGGAGACC AGGCGAAAGA CTCTAGTTGC ACTTTTCACT 
GATCACCAAT GGGTGGCGCC AGCAGTAGCT ACAGCAGACC TTCATGCTCA ATATGGATCT 
CCAACATATT TTTATGCTTT TTACCATCAT TGTCAGAGTG AAATGAAACC TAGCTGGTCG 
GATTCAGCAC ACGGAGACGA GGTGCCTTAT GTGTTTGGAA TTCCCATGCT TGGCCCCACT 
GATCTCTTTA ATTGTAATTT CTCCAAGAAT GATGTCATGC TTTCGGCGGT GGTGATGACG 
TACTGGACAA ATTTCGCTAA AACTGGGGAT CCGAACCAGC CAGTTCCACA GGACACCAAA 
TTCATACACA CAAAGCCCAA CCGCTTTGAG GAAGTAGCCT GGTCGAAATA CAACCCCAAA 
GACCAGCTTT ACTTACACAT CGGACTGAAG CCAAGGGTGA GAGATCACTA TCGCGCCACC 
AAGGTTGCTT TCTGGTTAGA ACTGGTACCT CATCTCCATA ACATCAACGA ACTCTTTCAG 
TATGTCTCGA CCACTACAAA GATACCTCCT CAGGATACTA CACCTTTCCC ATACACTAAA 
CGACTAGGTA AAACATGGCC ATCCACCACA CGACACCCTG GTGTTCCTCC TGCCAACACC 
AAACAAACCA CAGACCAGCG TAAAGGCAAT GATTTGAGTG ATGACTCAGC AGTGGTTATC 
GAGACAAAGC GCGATTACTC GACCGAACTC AGTGTGACCA TAGCAGTAGG TGCTTCTCTC 
CTTTTCCTCA ACATTCTGGC ATTCGCAGCG CTCTACTACA AGAAGGATAA ACGTCGACAT 
GAATCCAACC GACGAGGACC AAGCCCGCAG CGGAACTCTG CGCCAGCTAA TGTGGTGGCT 
AACGCTAATG ACATAGCACA TTTGCAGAGT GATGAGCTGA TGTCACTTCA GATGAAACAG 
CAGCAGATGG AACACGAGCA CCATCATGAG TGCGATTCTT TACAAGCTCA CGATACGCTT 
CGTCTTACCT GCCCGGCTGA TTATACGCTT ACCCTGCGAC GTTCACCTGA TGACATCCCA 
CTCATGACGC CAAGTACCAT AACAATGATT CCTAACACTC TGGCCGGAAT GCAGACCCTG 
CACAATTTCA ACACATTCGG AGGCAGTCAG AACAGTACCA ACTTGCCCCA TGGGCATTCC 
ACCACCAGGG TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330915.1
CDS	627..3161
Translation	MSRLRGTPWIPLTMVHPLATLRLFTWIVLAAAWLAITWAQQHPIVTTNYGKLRGLKTPLPNEILGPVEQYLGIPYALPPTGERRFQPPEPPMSWPGIRNATQFAPVCPQFLEDRFLLNDMLPVWFTANLDTVVTYVQDQSEDCLYLNIYVPTEDVGANKGDDFTNNEGGENKDIHDENGLRPVMVYIHGGSYMEGTGNMIDGSILASYGNVIVVTLNYRLGVLGFLSTGDQAAKGNYGLLDQIQALRWIKENIQAFKGDPKRVTIFGSGAGASCVSLLTLSHYSEDLFQKAIIQSGTALSSWAVNYQPAKYTRILAEKVGCNMLDSIDLVECLQNKNYKELIEQYITQAKYHIAFGPVIDGDVIPDDPQILMEQGEFLNYDIMLGVNQGEGFKFVDGIVDSEDGVSANDFDFAVSDFVDHLYGYPEGKDTLRETIKFMYTDWADKENPETRRKTLVALFTDHQWVAPAVATADLHAQYGSPTYFYAFYHHCQSEMKPSWSDSAHGDEVPYVFGIPMLGPTDLFNCNFSKNDVMLSAVVMTYWTNFAKTGDPNQPVPQDTKFIHTKPNRFEEVAWSKYNPKDQLYLHIGLKPRVRDHYRATKVAFWLELVPHLHNINELFQYVSTTTKIPPQDTTPFPYTKRLGKTWPSTTRHPGVPPANTKQTTDQRKGNDLSDDSAVVIETKRDYSTELSVTIAVGASLLFLNILAFAALYYKKDKRRHESNRRGPSPQRNSAPANVVANANDIAHLQSDELMSLQMKQQQMEHEHHHECDSLQAHDTLRLTCPADYTLTLRRSPDDIPLMTPSTITMIPNTLAGMQTLHNFNTFGGSQNSTNLPHGHSTTRV

Target ORF information:

RefSeq Version	XM_021475240.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neuroligin 4a (nlgn4a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475240.1

ORF Insert Sequence:

ATGTCACGGC TCAGAGGAAC CCCATGGATA CCACTTACCA TGGTTCATCC GCTGGCTACT 
CTACGCCTTT TTACCTGGAT TGTGCTTGCG GCTGCGTGGC TGGCCATTAC ATGGGCTCAG 
CAGCACCCTA TTGTTACGAC AAACTATGGC AAGCTAAGAG GCTTGAAAAC ACCTCTGCCC 
AATGAGATTC TAGGGCCCGT GGAACAGTAT TTGGGGATCC CCTATGCCCT GCCGCCCACA 
GGAGAAAGAA GGTTTCAGCC CCCAGAGCCT CCAATGTCCT GGCCAGGGAT TCGAAATGCT 
ACACAGTTTG CTCCTGTTTG TCCTCAGTTT CTGGAAGATC GTTTTTTACT CAATGATATG 
TTACCCGTGT GGTTCACCGC CAACTTGGAT ACTGTTGTAA CCTACGTACA AGATCAAAGT 
GAAGACTGCC TGTACTTAAA CATTTATGTT CCTACAGAAG ACGTAGGAGC CAACAAAGGC 
GATGATTTCA CCAATAATGA GGGTGGCGAA AATAAAGACA TCCATGATGA GAATGGACTG 
AGACCTGTTA TGGTGTACAT CCATGGTGGA TCCTACATGG AAGGAACAGG GAATATGATT 
GACGGCAGTA TTCTAGCCAG CTATGGAAAC GTCATTGTAG TGACCCTGAA CTATAGGCTG 
GGAGTGCTGG GGTTCCTGAG TACAGGTGAC CAGGCAGCCA AAGGGAATTA TGGGCTGTTG 
GACCAGATCC AAGCACTGCG GTGGATCAAA GAGAATATCC AGGCTTTCAA AGGCGACCCG 
AAAAGGGTGA CTATATTTGG ATCAGGAGCT GGGGCGTCCT GTGTTAGCTT GCTGACCCTA 
TCACACTATT CAGAAGATTT GTTTCAGAAA GCCATCATCC AGAGTGGGAC TGCTCTGTCT 
AGCTGGGCCG TCAACTACCA GCCTGCCAAA TACACCCGGA TACTAGCTGA GAAAGTGGGC 
TGTAATATGC TAGATAGCAT AGACCTGGTG GAATGTCTAC AGAACAAGAA CTACAAGGAG 
CTGATTGAAC AGTACATAAC ACAAGCCAAG TACCACATTG CGTTTGGTCC AGTAATTGAC 
GGTGACGTCA TCCCAGATGA CCCCCAAATT CTGATGGAGC AAGGGGAGTT CCTCAACTAT 
GACATCATGC TGGGTGTTAA CCAGGGTGAG GGTTTTAAGT TTGTGGATGG CATCGTGGAC 
AGTGAGGATG GTGTGTCTGC AAATGACTTT GACTTCGCCG TGTCTGATTT TGTGGACCAT 
CTTTATGGTT ATCCAGAGGG CAAGGACACG CTTCGAGAAA CCATCAAGTT CATGTATACT 
GACTGGGCTG ACAAGGAGAA CCCGGAGACC AGGCGAAAGA CTCTAGTTGC ACTTTTCACT 
GATCACCAAT GGGTGGCGCC AGCAGTAGCT ACAGCAGACC TTCATGCTCA ATATGGATCT 
CCAACATATT TTTATGCTTT TTACCATCAT TGTCAGAGTG AAATGAAACC TAGCTGGTCG 
GATTCAGCAC ACGGAGACGA GGTGCCTTAT GTGTTTGGAA TTCCCATGCT TGGCCCCACT 
GATCTCTTTA ATTGTAATTT CTCCAAGAAT GATGTCATGC TTTCGGCGGT GGTGATGACG 
TACTGGACAA ATTTCGCTAA AACTGGGGAT CCGAACCAGC CAGTTCCACA GGACACCAAA 
TTCATACACA CAAAGCCCAA CCGCTTTGAG GAAGTAGCCT GGTCGAAATA CAACCCCAAA 
GACCAGCTTT ACTTACACAT CGGACTGAAG CCAAGGGTGA GAGATCACTA TCGCGCCACC 
AAGGTTGCTT TCTGGTTAGA ACTGGTACCT CATCTCCATA ACATCAACGA ACTCTTTCAG 
TATGTCTCGA CCACTACAAA GATACCTCCT CAGGATACTA CACCTTTCCC ATACACTAAA 
CGACTAGGTA AAACATGGCC ATCCACCACA CGACACCCTG GTGTTCCTCC TGCCAACACC 
AAACAAACCA CAGACCAGCG TAAAGGCAAT GATTTGAGTG ATGACTCAGC AGTGGTTATC 
GAGACAAAGC GCGATTACTC GACCGAACTC AGTGTGACCA TAGCAGTAGG TGCTTCTCTC 
CTTTTCCTCA ACATTCTGGC ATTCGCAGCG CTCTACTACA AGAAGGATAA ACGTCGACAT 
GAATCCAACC GACGAGGACC AAGCCCGCAG CGGAACTCTG CGCCAGCTAA TGTGGTGGCT 
AACGCTAATG ACATAGCACA TTTGCAGAGT GATGAGCTGA TGTCACTTCA GATGAAACAG 
CAGCAGATGG AACACGAGCA CCATCATGAG TGCGATTCTT TACAAGCTCA CGATACGCTT 
CGTCTTACCT GCCCGGCTGA TTATACGCTT ACCCTGCGAC GTTCACCTGA TGACATCCCA 
CTCATGACGC CAAGTACCAT AACAATGATT CCTAACACTC TGGCCGGAAT GCAGACCCTG 
CACAATTTCA ACACATTCGG AGGCAGTCAG AACAGTACCA ACTTGCCCCA TGGGCATTCC 
ACCACCAGGG TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Characterization of the neuroligin gene family expression and evolution in zebrafish.
Developmental dynamics : an official publication of the American Association of Anatomists239(2)688-702(2010 Feb)
Rissone A,Sangiorgio L,Monopoli M,Beltrame M,Zucchi I,Bussolino F,Arese M,Cotelli F

Differential expression of neuroligin genes in the nervous system of zebrafish.
Developmental dynamics : an official publication of the American Association of Anatomists239(2)703-14(2010 Feb)
Davey C,Tallafuss A,Washbourne P

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nlgn4a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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