LRIG1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	LRIG1
Entrez Gene ID	560325
Full Name	leucine-rich repeats and immunoglobulin-like domains 1
Synonyms	cb994,wu:fc26a06
General protein information	Preferred Names leucine-rich repeats and immunoglobulin-like domains 1 Names leucine-rich repeats and immunoglobulin-like domains protein 1 fc26a06
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_683725.9	XP_688817.4	leucine-rich repeats and immunoglobulin-like domains protein 1 isoform X1
XM_021474233.1	XP_021329908.1	leucine-rich repeats and immunoglobulin-like domains protein 1 isoform X3
XM_009305941.3	XP_009304216.1	leucine-rich repeats and immunoglobulin-like domains protein 1 isoform X2
XM_009305941.2	XP_009304216.1	leucine-rich repeats and immunoglobulin-like domains protein 1 isoform X2
XM_683725.8	XP_688817.4	leucine-rich repeats and immunoglobulin-like domains protein 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	LRIG1	NP_056356.2
Pan troglodytes (chimpanzee)	LRIG1	XP_516568.3
Bos taurus (cattle)	LRIG1	XP_002697008.1
Mus musculus (house mouse)	Lrig1	NP_032403.2
Rattus norvegicus (Norway rat)	Lrig1	XP_232237.6
Canis lupus familiaris (dog)	LRIG1	XP_005632338.1
Gallus gallus (chicken)	LRIG1	XP_425165.4
Danio rerio (zebrafish)	lrig1	XP_688817.4
Xenopus tropicalis (tropical clawed frog)	lrig1	XP_002938412.1
	LRIG1	XP_002802785.1

Gene Ontology

The following LRIG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LRIG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa43265	XM_009305941.3 Latest version!	Danio rerio leucine-rich repeats and immunoglobulin-like domains 1 (lrig1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa146634	XM_021474233.1 Latest version!	Danio rerio leucine-rich repeats and immunoglobulin-like domains 1 (lrig1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
ODa16957	XM_683725.9 Latest version!	Danio rerio leucine-rich repeats and immunoglobulin-like domains 1 (lrig1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa43265	XM_009305941.2	Danio rerio leucine-rich repeats and immunoglobulin-like domains 1 (lrig1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa16957	XM_683725.8	Danio rerio leucine-rich repeats and immunoglobulin-like domains 1 (lrig1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LRIG1 ( XM_009305941.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009304216.1 Danio rerio leucine-rich repeats and immunoglobulin-like domains 1 (lrig1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa43265
Clone ID Related Accession (Same CDS sequence)	XM_009305941.3 , XM_009305941.2
Accession Version	XM_009305941.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	leucine-rich repeats and immunoglobulin-like domains protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009305941.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGT GGCTGGGACA GATGGGATCA CTTTCATATC ATGTGTTTTA TTGGCTTTTG 
ACGCTGGAAC TGTTAATCAA TTATGGCGCA CCGTGTCCTC AAAACTGTAC GTGTACGCTT 
GATACGACCG ACTGCAGTCG TTTGGACATG ACTGATGTCC CTCAAGACTT GCCAAAGAGC 
ACTGTGCATC TAAATCTGAG CCACAACAAG CTGACGGCAG TCGATATGGA CATCCTCTCC 
AATCTGCCCC ACCTGAGAGA AGTGAGGCTT GACCACAATG AGCTGACATC TATCCCTTCA 
TTTGGAGATG CCGCAGCATC AGTTGTTACA CTTCTCCTGC ACCATAATAG AATCCGCAGA 
CTGGAGGGGA GTCTTCTACA GAACTTCTCT GCTCTTGAGA CGCTAGATTT GAGTAATAAT 
GACATCACAG AGTTGAGAGA GCACTGCTTC CCTCTGGGGT TACAAATCAA AGACCTGCAC 
CTGAGCAGTA ATAAGATCGT TCATCTGGAG TTCGGGGCAT TTAAGAATCT TGCCGGGAGT 
CTGCAGATCC TCAGATTGAG CCGGAACCGA TTAACTCATC TTCCTGTTAA AGGCCTGGAG 
CTGCCCAAAC TCACACAACT GGAGTTGAGT AGGAACAGGT TGCGGTTGAT CGAGGGACTC 
ACCTTCCAAG GTTTGAGCAG TTTAGAAGTT CTCAAGCTTC AGAGAAACAA CATCAGCAAA 
CTAACAGATG GGGCATTCTG GGGTCTGGCC CGGATGAGGG TTCTGCACCT GGACTATAAC 
AGTCTACGAG AAGTGAACAG TGGCTCTCTG TATGGTCTGG AGTCTCTGCT GCAGCTCTAC 
CTCGCCAACA ATTCCATCTC AAACTTTAAC CCTGAGGGTT GGGGCTTTTG TGAGAGACTT 
CGAGAACTGA ATTTGTCTTA TAATAACCTG ACGAAGTTGT CTGAGGGTAG TTTTGCCAAG 
CTGGTAAACC TGATTTCCCT GCGTTTGGGA CACAATTCCA TCAGTCACAT CACTGAAGGA 
GCATTCAGAG GCCTTAGCTC CCTGCGCACA CTGGAGTTGG ACCACAATGA CATCTCTGGA 
ACTATTGAGG ACACCAGTGG GGCCTTCACT GGCCTCGAAA ACCTCAACAA GCTAACTCTG 
TTTGGAAACA AGATCAAGTC GGTGGCCAAG AAAGCCTTCT CTGGCCTGGA GGCTCTGGAG 
CACCTGAATT TAGGGGAAAA TGCCATCCGC TCCATCCAAC CCGAGGCATT CAGCAAGATG 
AAGAACCTGC GCTACCTTCA TATTCAGAGC GACAGTTTTC TGTGTGACTG TCAGCTGCAC 
TGGTTTCCAG ATTGGCTCCT CACTCGAGGG CTGAACCCTG GTGTTCAGGC CACATGTGCC 
CATCCAGAGA GCCTAAAGGG CACCAGCATC TACCAAGCCC CACCACAGAG CTTTGTTTGT 
GATGACCTTC CCAAACCCCA GATCACGGTT CACCCAGTCA CCACAGCTGC AGTATTGGGT 
AAAGACGTGC GTCTGACCTG TACTGCTGCC AGCAGCAGCA GTTCTCCCAT GACCTTCACC 
TGGCTAAAGG ACCAGGAGAC ACTCCGCCAG GCTGAGGTGG AGAACTTTGC CCATGTGCGG 
GCCAGCGATG GAGGAGTGAT GGAGTACACT ACGATCCTTC ACCTTCGACA CGTCACCTTC 
AACCATGAGG GACGATACCA GTGCATCATC AGCAATCACT TCGGCTCCTC TTACTCCAAC 
AAGGCCCGCG TCACTGTAAA CGTCCTGCCT TCGTTCGTCA AGACCCCACG TGACATCACC 
ATCCGCACAG GCACCAAAGC GCGGTTGGAG TGTGCGGCCG AAGGGCATCC GACTCCACAG 
GTGGCATGGC AGAAAGATGG CGGAACAGAC TTCCCTGCTG CCCGAGAGCG GCGCATGCGT 
GTGATGCCTG ATGATGATGT GTTCTTTATC ATGGATGTGA AGCCAGAGGA TATGGGTGTT 
TACAGCTGCA CAGCGAAAAA CACAGCAGGC ATGATCTCCG CCAACGTCAC CTTAACAGTG 
CTAGAGACCC CTCATCTGGC ACAGGAAATG GAGGATCGTA ATGTGGTGGT AGGCGAAACT 
GTAGCACTGC AGTGCAAGGC TTTGGGCAGT CCACCACCTC GCATCACCTG GCTGAAGGGT 
GAAGAGCCTC TTCGAGCCAG CGACCGTCAT CACTTCACTC CAGGAAATCA GCTGTTGGTG 
ATCGGCAGCA GCACATTAGA GGATGCTGGA CGCTACACTT GTGTGATGTC CAATACGCTC 
GGCACCGAAC GTGCTCACTC GCAGCTCACT GTCTACCAGA GATTTGAGGA GTGCGCACCT 
TTTACCAGCC CCAGCACTGT TACTGTAGGC ATCATCATCA TCGCTGTGGT CACCAGTATT 
GTGGTGACTT CACTAGTTTG GGTGTGTATT ATCTATCAGA CCAGGAAGAA GAGCGAGGAG 
TGCAGTGTAA ACACAGATGA GACGATTGTC CCTCCTGATG TTCCCAGTTA CCTGTCCTCT 
CAGGGCACTT TATCAGAAAG ACAGGACGTG TGTATACGAG TGGAGTCCAA TGGGGGATCT 
CAGCCCAATG GACGAATAGA AAGCAATGGC TACGATGTGC CGGTCGTGTG CGCAGACTGC 
TTGGAAAACA GCACCAGCTA CTCCAAACAC TGTAACTACC TCCCTCACGA TTTAGCGCCC 
CCTTCAGGCA TGGAGTATCA GCAGACGCAC CAAACACCCA TCTTTCCCAC CAACCACGAC 
AGGGTGACAA TATCACCCTC ATCAAACCAG TACAGTGACC GAAATGGATT GTTGGTGAAC 
AAGTCAGACA CACCACTGTC ACTGGAGGAG TCGTACCATC GGCCAGTAAA ACTGCTGTCT 
GAGGACTGTG ACATTGGGTC AGAGCTCACA CAGACTCTAT TACCCAACGG ACACACACAC 
GCGCCCAGTT CAGCTCTAAA CCAGAGCCAA TCCTTTAGGC GGGACAGTGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304216.1
CDS	928..3921
Translation	MAAWLGQMGSLSYHVFYWLLTLELLINYGAPCPQNCTCTLDTTDCSRLDMTDVPQDLPKSTVHLNLSHNKLTAVDMDILSNLPHLREVRLDHNELTSIPSFGDAAASVVTLLLHHNRIRRLEGSLLQNFSALETLDLSNNDITELREHCFPLGLQIKDLHLSSNKIVHLEFGAFKNLAGSLQILRLSRNRLTHLPVKGLELPKLTQLELSRNRLRLIEGLTFQGLSSLEVLKLQRNNISKLTDGAFWGLARMRVLHLDYNSLREVNSGSLYGLESLLQLYLANNSISNFNPEGWGFCERLRELNLSYNNLTKLSEGSFAKLVNLISLRLGHNSISHITEGAFRGLSSLRTLELDHNDISGTIEDTSGAFTGLENLNKLTLFGNKIKSVAKKAFSGLEALEHLNLGENAIRSIQPEAFSKMKNLRYLHIQSDSFLCDCQLHWFPDWLLTRGLNPGVQATCAHPESLKGTSIYQAPPQSFVCDDLPKPQITVHPVTTAAVLGKDVRLTCTAASSSSSPMTFTWLKDQETLRQAEVENFAHVRASDGGVMEYTTILHLRHVTFNHEGRYQCIISNHFGSSYSNKARVTVNVLPSFVKTPRDITIRTGTKARLECAAEGHPTPQVAWQKDGGTDFPAARERRMRVMPDDDVFFIMDVKPEDMGVYSCTAKNTAGMISANVTLTVLETPHLAQEMEDRNVVVGETVALQCKALGSPPPRITWLKGEEPLRASDRHHFTPGNQLLVIGSSTLEDAGRYTCVMSNTLGTERAHSQLTVYQRFEECAPFTSPSTVTVGIIIIAVVTSIVVTSLVWVCIIYQTRKKSEECSVNTDETIVPPDVPSYLSSQGTLSERQDVCIRVESNGGSQPNGRIESNGYDVPVVCADCLENSTSYSKHCNYLPHDLAPPSGMEYQQTHQTPIFPTNHDRVTISPSSNQYSDRNGLLVNKSDTPLSLEESYHRPVKLLSEDCDIGSELTQTLLPNGHTHAPSSALNQSQSFRRDSD

Target ORF information:

RefSeq Version	XM_009305941.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio leucine-rich repeats and immunoglobulin-like domains 1 (lrig1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305941.3

ORF Insert Sequence:

ATGGCGGCGT GGCTGGGACA GATGGGATCA CTTTCATATC ATGTGTTTTA TTGGCTTTTG 
ACGCTGGAAC TGTTAATCAA TTATGGCGCA CCGTGTCCTC AAAACTGTAC GTGTACGCTT 
GATACGACCG ACTGCAGTCG TTTGGACATG ACTGATGTCC CTCAAGACTT GCCAAAGAGC 
ACTGTGCATC TAAATCTGAG CCACAACAAG CTGACGGCAG TCGATATGGA CATCCTCTCC 
AATCTGCCCC ACCTGAGAGA AGTGAGGCTT GACCACAATG AGCTGACATC TATCCCTTCA 
TTTGGAGATG CCGCAGCATC AGTTGTTACA CTTCTCCTGC ACCATAATAG AATCCGCAGA 
CTGGAGGGGA GTCTTCTACA GAACTTCTCT GCTCTTGAGA CGCTAGATTT GAGTAATAAT 
GACATCACAG AGTTGAGAGA GCACTGCTTC CCTCTGGGGT TACAAATCAA AGACCTGCAC 
CTGAGCAGTA ATAAGATCGT TCATCTGGAG TTCGGGGCAT TTAAGAATCT TGCCGGGAGT 
CTGCAGATCC TCAGATTGAG CCGGAACCGA TTAACTCATC TTCCTGTTAA AGGCCTGGAG 
CTGCCCAAAC TCACACAACT GGAGTTGAGT AGGAACAGGT TGCGGTTGAT CGAGGGACTC 
ACCTTCCAAG GTTTGAGCAG TTTAGAAGTT CTCAAGCTTC AGAGAAACAA CATCAGCAAA 
CTAACAGATG GGGCATTCTG GGGTCTGGCC CGGATGAGGG TTCTGCACCT GGACTATAAC 
AGTCTACGAG AAGTGAACAG TGGCTCTCTG TATGGTCTGG AGTCTCTGCT GCAGCTCTAC 
CTCGCCAACA ATTCCATCTC AAACTTTAAC CCTGAGGGTT GGGGCTTTTG TGAGAGACTT 
CGAGAACTGA ATTTGTCTTA TAATAACCTG ACGAAGTTGT CTGAGGGTAG TTTTGCCAAG 
CTGGTAAACC TGATTTCCCT GCGTTTGGGA CACAATTCCA TCAGTCACAT CACTGAAGGA 
GCATTCAGAG GCCTTAGCTC CCTGCGCACA CTGGAGTTGG ACCACAATGA CATCTCTGGA 
ACTATTGAGG ACACCAGTGG GGCCTTCACT GGCCTCGAAA ACCTCAACAA GCTAACTCTG 
TTTGGAAACA AGATCAAGTC GGTGGCCAAG AAAGCCTTCT CTGGCCTGGA GGCTCTGGAG 
CACCTGAATT TAGGGGAAAA TGCCATCCGC TCCATCCAAC CCGAGGCATT CAGCAAGATG 
AAGAACCTGC GCTACCTTCA TATTCAGAGC GACAGTTTTC TGTGTGACTG TCAGCTGCAC 
TGGTTTCCAG ATTGGCTCCT CACTCGAGGG CTGAACCCTG GTGTTCAGGC CACATGTGCC 
CATCCAGAGA GCCTAAAGGG CACCAGCATC TACCAAGCCC CACCACAGAG CTTTGTTTGT 
GATGACCTTC CCAAACCCCA GATCACGGTT CACCCAGTCA CCACAGCTGC AGTATTGGGT 
AAAGACGTGC GTCTGACCTG TACTGCTGCC AGCAGCAGCA GTTCTCCCAT GACCTTCACC 
TGGCTAAAGG ACCAGGAGAC ACTCCGCCAG GCTGAGGTGG AGAACTTTGC CCATGTGCGG 
GCCAGCGATG GAGGAGTGAT GGAGTACACT ACGATCCTTC ACCTTCGACA CGTCACCTTC 
AACCATGAGG GACGATACCA GTGCATCATC AGCAATCACT TCGGCTCCTC TTACTCCAAC 
AAGGCCCGCG TCACTGTAAA CGTCCTGCCT TCGTTCGTCA AGACCCCACG TGACATCACC 
ATCCGCACAG GCACCAAAGC GCGGTTGGAG TGTGCGGCCG AAGGGCATCC GACTCCACAG 
GTGGCATGGC AGAAAGATGG CGGAACAGAC TTCCCTGCTG CCCGAGAGCG GCGCATGCGT 
GTGATGCCTG ATGATGATGT GTTCTTTATC ATGGATGTGA AGCCAGAGGA TATGGGTGTT 
TACAGCTGCA CAGCGAAAAA CACAGCAGGC ATGATCTCCG CCAACGTCAC CTTAACAGTG 
CTAGAGACCC CTCATCTGGC ACAGGAAATG GAGGATCGTA ATGTGGTGGT AGGCGAAACT 
GTAGCACTGC AGTGCAAGGC TTTGGGCAGT CCACCACCTC GCATCACCTG GCTGAAGGGT 
GAAGAGCCTC TTCGAGCCAG CGACCGTCAT CACTTCACTC CAGGAAATCA GCTGTTGGTG 
ATCGGCAGCA GCACATTAGA GGATGCTGGA CGCTACACTT GTGTGATGTC CAATACGCTC 
GGCACCGAAC GTGCTCACTC GCAGCTCACT GTCTACCAGA GATTTGAGGA GTGCGCACCT 
TTTACCAGCC CCAGCACTGT TACTGTAGGC ATCATCATCA TCGCTGTGGT CACCAGTATT 
GTGGTGACTT CACTAGTTTG GGTGTGTATT ATCTATCAGA CCAGGAAGAA GAGCGAGGAG 
TGCAGTGTAA ACACAGATGA GACGATTGTC CCTCCTGATG TTCCCAGTTA CCTGTCCTCT 
CAGGGCACTT TATCAGAAAG ACAGGACGTG TGTATACGAG TGGAGTCCAA TGGGGGATCT 
CAGCCCAATG GACGAATAGA AAGCAATGGC TACGATGTGC CGGTCGTGTG CGCAGACTGC 
TTGGAAAACA GCACCAGCTA CTCCAAACAC TGTAACTACC TCCCTCACGA TTTAGCGCCC 
CCTTCAGGCA TGGAGTATCA GCAGACGCAC CAAACACCCA TCTTTCCCAC CAACCACGAC 
AGGGTGACAA TATCACCCTC ATCAAACCAG TACAGTGACC GAAATGGATT GTTGGTGAAC 
AAGTCAGACA CACCACTGTC ACTGGAGGAG TCGTACCATC GGCCAGTAAA ACTGCTGTCT 
GAGGACTGTG ACATTGGGTC AGAGCTCACA CAGACTCTAT TACCCAACGG ACACACACAC 
GCGCCCAGTT CAGCTCTAAA CCAGAGCCAA TCCTTTAGGC GGGACAGTGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRIG1 ( XM_009305941.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009304216.1 Danio rerio leucine-rich repeats and immunoglobulin-like domains 1 (lrig1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa43265
Clone ID Related Accession (Same CDS sequence)	XM_009305941.3 , XM_009305941.2
Accession Version	XM_009305941.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	leucine-rich repeats and immunoglobulin-like domains protein 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305941.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGT GGCTGGGACA GATGGGATCA CTTTCATATC ATGTGTTTTA TTGGCTTTTG 
ACGCTGGAAC TGTTAATCAA TTATGGCGCA CCGTGTCCTC AAAACTGTAC GTGTACGCTT 
GATACGACCG ACTGCAGTCG TTTGGACATG ACTGATGTCC CTCAAGACTT GCCAAAGAGC 
ACTGTGCATC TAAATCTGAG CCACAACAAG CTGACGGCAG TCGATATGGA CATCCTCTCC 
AATCTGCCCC ACCTGAGAGA AGTGAGGCTT GACCACAATG AGCTGACATC TATCCCTTCA 
TTTGGAGATG CCGCAGCATC AGTTGTTACA CTTCTCCTGC ACCATAATAG AATCCGCAGA 
CTGGAGGGGA GTCTTCTACA GAACTTCTCT GCTCTTGAGA CGCTAGATTT GAGTAATAAT 
GACATCACAG AGTTGAGAGA GCACTGCTTC CCTCTGGGGT TACAAATCAA AGACCTGCAC 
CTGAGCAGTA ATAAGATCGT TCATCTGGAG TTCGGGGCAT TTAAGAATCT TGCCGGGAGT 
CTGCAGATCC TCAGATTGAG CCGGAACCGA TTAACTCATC TTCCTGTTAA AGGCCTGGAG 
CTGCCCAAAC TCACACAACT GGAGTTGAGT AGGAACAGGT TGCGGTTGAT CGAGGGACTC 
ACCTTCCAAG GTTTGAGCAG TTTAGAAGTT CTCAAGCTTC AGAGAAACAA CATCAGCAAA 
CTAACAGATG GGGCATTCTG GGGTCTGGCC CGGATGAGGG TTCTGCACCT GGACTATAAC 
AGTCTACGAG AAGTGAACAG TGGCTCTCTG TATGGTCTGG AGTCTCTGCT GCAGCTCTAC 
CTCGCCAACA ATTCCATCTC AAACTTTAAC CCTGAGGGTT GGGGCTTTTG TGAGAGACTT 
CGAGAACTGA ATTTGTCTTA TAATAACCTG ACGAAGTTGT CTGAGGGTAG TTTTGCCAAG 
CTGGTAAACC TGATTTCCCT GCGTTTGGGA CACAATTCCA TCAGTCACAT CACTGAAGGA 
GCATTCAGAG GCCTTAGCTC CCTGCGCACA CTGGAGTTGG ACCACAATGA CATCTCTGGA 
ACTATTGAGG ACACCAGTGG GGCCTTCACT GGCCTCGAAA ACCTCAACAA GCTAACTCTG 
TTTGGAAACA AGATCAAGTC GGTGGCCAAG AAAGCCTTCT CTGGCCTGGA GGCTCTGGAG 
CACCTGAATT TAGGGGAAAA TGCCATCCGC TCCATCCAAC CCGAGGCATT CAGCAAGATG 
AAGAACCTGC GCTACCTTCA TATTCAGAGC GACAGTTTTC TGTGTGACTG TCAGCTGCAC 
TGGTTTCCAG ATTGGCTCCT CACTCGAGGG CTGAACCCTG GTGTTCAGGC CACATGTGCC 
CATCCAGAGA GCCTAAAGGG CACCAGCATC TACCAAGCCC CACCACAGAG CTTTGTTTGT 
GATGACCTTC CCAAACCCCA GATCACGGTT CACCCAGTCA CCACAGCTGC AGTATTGGGT 
AAAGACGTGC GTCTGACCTG TACTGCTGCC AGCAGCAGCA GTTCTCCCAT GACCTTCACC 
TGGCTAAAGG ACCAGGAGAC ACTCCGCCAG GCTGAGGTGG AGAACTTTGC CCATGTGCGG 
GCCAGCGATG GAGGAGTGAT GGAGTACACT ACGATCCTTC ACCTTCGACA CGTCACCTTC 
AACCATGAGG GACGATACCA GTGCATCATC AGCAATCACT TCGGCTCCTC TTACTCCAAC 
AAGGCCCGCG TCACTGTAAA CGTCCTGCCT TCGTTCGTCA AGACCCCACG TGACATCACC 
ATCCGCACAG GCACCAAAGC GCGGTTGGAG TGTGCGGCCG AAGGGCATCC GACTCCACAG 
GTGGCATGGC AGAAAGATGG CGGAACAGAC TTCCCTGCTG CCCGAGAGCG GCGCATGCGT 
GTGATGCCTG ATGATGATGT GTTCTTTATC ATGGATGTGA AGCCAGAGGA TATGGGTGTT 
TACAGCTGCA CAGCGAAAAA CACAGCAGGC ATGATCTCCG CCAACGTCAC CTTAACAGTG 
CTAGAGACCC CTCATCTGGC ACAGGAAATG GAGGATCGTA ATGTGGTGGT AGGCGAAACT 
GTAGCACTGC AGTGCAAGGC TTTGGGCAGT CCACCACCTC GCATCACCTG GCTGAAGGGT 
GAAGAGCCTC TTCGAGCCAG CGACCGTCAT CACTTCACTC CAGGAAATCA GCTGTTGGTG 
ATCGGCAGCA GCACATTAGA GGATGCTGGA CGCTACACTT GTGTGATGTC CAATACGCTC 
GGCACCGAAC GTGCTCACTC GCAGCTCACT GTCTACCAGA GATTTGAGGA GTGCGCACCT 
TTTACCAGCC CCAGCACTGT TACTGTAGGC ATCATCATCA TCGCTGTGGT CACCAGTATT 
GTGGTGACTT CACTAGTTTG GGTGTGTATT ATCTATCAGA CCAGGAAGAA GAGCGAGGAG 
TGCAGTGTAA ACACAGATGA GACGATTGTC CCTCCTGATG TTCCCAGTTA CCTGTCCTCT 
CAGGGCACTT TATCAGAAAG ACAGGACGTG TGTATACGAG TGGAGTCCAA TGGGGGATCT 
CAGCCCAATG GACGAATAGA AAGCAATGGC TACGATGTGC CGGTCGTGTG CGCAGACTGC 
TTGGAAAACA GCACCAGCTA CTCCAAACAC TGTAACTACC TCCCTCACGA TTTAGCGCCC 
CCTTCAGGCA TGGAGTATCA GCAGACGCAC CAAACACCCA TCTTTCCCAC CAACCACGAC 
AGGGTGACAA TATCACCCTC ATCAAACCAG TACAGTGACC GAAATGGATT GTTGGTGAAC 
AAGTCAGACA CACCACTGTC ACTGGAGGAG TCGTACCATC GGCCAGTAAA ACTGCTGTCT 
GAGGACTGTG ACATTGGGTC AGAGCTCACA CAGACTCTAT TACCCAACGG ACACACACAC 
GCGCCCAGTT CAGCTCTAAA CCAGAGCCAA TCCTTTAGGC GGGACAGTGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304216.1
CDS	922..3915
Translation	MAAWLGQMGSLSYHVFYWLLTLELLINYGAPCPQNCTCTLDTTDCSRLDMTDVPQDLPKSTVHLNLSHNKLTAVDMDILSNLPHLREVRLDHNELTSIPSFGDAAASVVTLLLHHNRIRRLEGSLLQNFSALETLDLSNNDITELREHCFPLGLQIKDLHLSSNKIVHLEFGAFKNLAGSLQILRLSRNRLTHLPVKGLELPKLTQLELSRNRLRLIEGLTFQGLSSLEVLKLQRNNISKLTDGAFWGLARMRVLHLDYNSLREVNSGSLYGLESLLQLYLANNSISNFNPEGWGFCERLRELNLSYNNLTKLSEGSFAKLVNLISLRLGHNSISHITEGAFRGLSSLRTLELDHNDISGTIEDTSGAFTGLENLNKLTLFGNKIKSVAKKAFSGLEALEHLNLGENAIRSIQPEAFSKMKNLRYLHIQSDSFLCDCQLHWFPDWLLTRGLNPGVQATCAHPESLKGTSIYQAPPQSFVCDDLPKPQITVHPVTTAAVLGKDVRLTCTAASSSSSPMTFTWLKDQETLRQAEVENFAHVRASDGGVMEYTTILHLRHVTFNHEGRYQCIISNHFGSSYSNKARVTVNVLPSFVKTPRDITIRTGTKARLECAAEGHPTPQVAWQKDGGTDFPAARERRMRVMPDDDVFFIMDVKPEDMGVYSCTAKNTAGMISANVTLTVLETPHLAQEMEDRNVVVGETVALQCKALGSPPPRITWLKGEEPLRASDRHHFTPGNQLLVIGSSTLEDAGRYTCVMSNTLGTERAHSQLTVYQRFEECAPFTSPSTVTVGIIIIAVVTSIVVTSLVWVCIIYQTRKKSEECSVNTDETIVPPDVPSYLSSQGTLSERQDVCIRVESNGGSQPNGRIESNGYDVPVVCADCLENSTSYSKHCNYLPHDLAPPSGMEYQQTHQTPIFPTNHDRVTISPSSNQYSDRNGLLVNKSDTPLSLEESYHRPVKLLSEDCDIGSELTQTLLPNGHTHAPSSALNQSQSFRRDSD

Target ORF information:

RefSeq Version	XM_009305941.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio leucine-rich repeats and immunoglobulin-like domains 1 (lrig1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305941.2

ORF Insert Sequence:

ATGGCGGCGT GGCTGGGACA GATGGGATCA CTTTCATATC ATGTGTTTTA TTGGCTTTTG 
ACGCTGGAAC TGTTAATCAA TTATGGCGCA CCGTGTCCTC AAAACTGTAC GTGTACGCTT 
GATACGACCG ACTGCAGTCG TTTGGACATG ACTGATGTCC CTCAAGACTT GCCAAAGAGC 
ACTGTGCATC TAAATCTGAG CCACAACAAG CTGACGGCAG TCGATATGGA CATCCTCTCC 
AATCTGCCCC ACCTGAGAGA AGTGAGGCTT GACCACAATG AGCTGACATC TATCCCTTCA 
TTTGGAGATG CCGCAGCATC AGTTGTTACA CTTCTCCTGC ACCATAATAG AATCCGCAGA 
CTGGAGGGGA GTCTTCTACA GAACTTCTCT GCTCTTGAGA CGCTAGATTT GAGTAATAAT 
GACATCACAG AGTTGAGAGA GCACTGCTTC CCTCTGGGGT TACAAATCAA AGACCTGCAC 
CTGAGCAGTA ATAAGATCGT TCATCTGGAG TTCGGGGCAT TTAAGAATCT TGCCGGGAGT 
CTGCAGATCC TCAGATTGAG CCGGAACCGA TTAACTCATC TTCCTGTTAA AGGCCTGGAG 
CTGCCCAAAC TCACACAACT GGAGTTGAGT AGGAACAGGT TGCGGTTGAT CGAGGGACTC 
ACCTTCCAAG GTTTGAGCAG TTTAGAAGTT CTCAAGCTTC AGAGAAACAA CATCAGCAAA 
CTAACAGATG GGGCATTCTG GGGTCTGGCC CGGATGAGGG TTCTGCACCT GGACTATAAC 
AGTCTACGAG AAGTGAACAG TGGCTCTCTG TATGGTCTGG AGTCTCTGCT GCAGCTCTAC 
CTCGCCAACA ATTCCATCTC AAACTTTAAC CCTGAGGGTT GGGGCTTTTG TGAGAGACTT 
CGAGAACTGA ATTTGTCTTA TAATAACCTG ACGAAGTTGT CTGAGGGTAG TTTTGCCAAG 
CTGGTAAACC TGATTTCCCT GCGTTTGGGA CACAATTCCA TCAGTCACAT CACTGAAGGA 
GCATTCAGAG GCCTTAGCTC CCTGCGCACA CTGGAGTTGG ACCACAATGA CATCTCTGGA 
ACTATTGAGG ACACCAGTGG GGCCTTCACT GGCCTCGAAA ACCTCAACAA GCTAACTCTG 
TTTGGAAACA AGATCAAGTC GGTGGCCAAG AAAGCCTTCT CTGGCCTGGA GGCTCTGGAG 
CACCTGAATT TAGGGGAAAA TGCCATCCGC TCCATCCAAC CCGAGGCATT CAGCAAGATG 
AAGAACCTGC GCTACCTTCA TATTCAGAGC GACAGTTTTC TGTGTGACTG TCAGCTGCAC 
TGGTTTCCAG ATTGGCTCCT CACTCGAGGG CTGAACCCTG GTGTTCAGGC CACATGTGCC 
CATCCAGAGA GCCTAAAGGG CACCAGCATC TACCAAGCCC CACCACAGAG CTTTGTTTGT 
GATGACCTTC CCAAACCCCA GATCACGGTT CACCCAGTCA CCACAGCTGC AGTATTGGGT 
AAAGACGTGC GTCTGACCTG TACTGCTGCC AGCAGCAGCA GTTCTCCCAT GACCTTCACC 
TGGCTAAAGG ACCAGGAGAC ACTCCGCCAG GCTGAGGTGG AGAACTTTGC CCATGTGCGG 
GCCAGCGATG GAGGAGTGAT GGAGTACACT ACGATCCTTC ACCTTCGACA CGTCACCTTC 
AACCATGAGG GACGATACCA GTGCATCATC AGCAATCACT TCGGCTCCTC TTACTCCAAC 
AAGGCCCGCG TCACTGTAAA CGTCCTGCCT TCGTTCGTCA AGACCCCACG TGACATCACC 
ATCCGCACAG GCACCAAAGC GCGGTTGGAG TGTGCGGCCG AAGGGCATCC GACTCCACAG 
GTGGCATGGC AGAAAGATGG CGGAACAGAC TTCCCTGCTG CCCGAGAGCG GCGCATGCGT 
GTGATGCCTG ATGATGATGT GTTCTTTATC ATGGATGTGA AGCCAGAGGA TATGGGTGTT 
TACAGCTGCA CAGCGAAAAA CACAGCAGGC ATGATCTCCG CCAACGTCAC CTTAACAGTG 
CTAGAGACCC CTCATCTGGC ACAGGAAATG GAGGATCGTA ATGTGGTGGT AGGCGAAACT 
GTAGCACTGC AGTGCAAGGC TTTGGGCAGT CCACCACCTC GCATCACCTG GCTGAAGGGT 
GAAGAGCCTC TTCGAGCCAG CGACCGTCAT CACTTCACTC CAGGAAATCA GCTGTTGGTG 
ATCGGCAGCA GCACATTAGA GGATGCTGGA CGCTACACTT GTGTGATGTC CAATACGCTC 
GGCACCGAAC GTGCTCACTC GCAGCTCACT GTCTACCAGA GATTTGAGGA GTGCGCACCT 
TTTACCAGCC CCAGCACTGT TACTGTAGGC ATCATCATCA TCGCTGTGGT CACCAGTATT 
GTGGTGACTT CACTAGTTTG GGTGTGTATT ATCTATCAGA CCAGGAAGAA GAGCGAGGAG 
TGCAGTGTAA ACACAGATGA GACGATTGTC CCTCCTGATG TTCCCAGTTA CCTGTCCTCT 
CAGGGCACTT TATCAGAAAG ACAGGACGTG TGTATACGAG TGGAGTCCAA TGGGGGATCT 
CAGCCCAATG GACGAATAGA AAGCAATGGC TACGATGTGC CGGTCGTGTG CGCAGACTGC 
TTGGAAAACA GCACCAGCTA CTCCAAACAC TGTAACTACC TCCCTCACGA TTTAGCGCCC 
CCTTCAGGCA TGGAGTATCA GCAGACGCAC CAAACACCCA TCTTTCCCAC CAACCACGAC 
AGGGTGACAA TATCACCCTC ATCAAACCAG TACAGTGACC GAAATGGATT GTTGGTGAAC 
AAGTCAGACA CACCACTGTC ACTGGAGGAG TCGTACCATC GGCCAGTAAA ACTGCTGTCT 
GAGGACTGTG ACATTGGGTC AGAGCTCACA CAGACTCTAT TACCCAACGG ACACACACAC 
GCGCCCAGTT CAGCTCTAAA CCAGAGCCAA TCCTTTAGGC GGGACAGTGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRIG1 ( XM_021474233.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021329908.1 Danio rerio leucine-rich repeats and immunoglobulin-like domains 1 (lrig1), transcript variant X3, mRNA.

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CloneID	ODa146634
Clone ID Related Accession (Same CDS sequence)	XM_021474233.1
Accession Version	XM_021474233.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	leucine-rich repeats and immunoglobulin-like domains protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395297.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTTCA CCTGGCTAAA GGACCAGGAG ACACTCCGCC AGGCTGAGGT GGAGAACTTT 
GCCCATGTGC GGGCCAGCGA TGGAGGAGTG ATGGAGTACA CTACGATCCT TCACCTTCGA 
CACGTCACCT TCAACCATGA GGGACGATAC CAGTGCATCA TCAGCAATCA CTTCGGCTCC 
TCTTACTCCA ACAAGGCCCG CGTCACTGTA AACGTCCTGC CTTCGTTCGT CAAGACCCCA 
CGTGACATCA CCATCCGCAC AGGCACCAAA GCGCGGTTGG AGTGTGCGGC CGAAGGGCAT 
CCGACTCCAC AGGTGGCATG GCAGAAAGAT GGCGGAACAG ACTTCCCTGC TGCCCGAGAG 
CGGCGCATGC GTGTGATGCC TGATGATGAT GTGTTCTTTA TCATGGATGT GAAGCCAGAG 
GATATGGGTG TTTACAGCTG CACAGCGAAA AACACAGCAG GCATGATCTC CGCCAACGTC 
ACCTTAACAG TGCTAGAGAC CCCTCATCTG GCACAGGAAA TGGAGGATCG TAATGTGGTG 
GTAGGCGAAA CTGTAGCACT GCAGTGCAAG GCTTTGGGCA GTCCACCACC TCGCATCACC 
TGGCTGAAGG GTGAAGAGCC TCTTCGAGCC AGCGACCGTC ATCACTTCAC TCCAGGAAAT 
CAGCTGTTGG TGATCGGCAG CAGCACATTA GAGGATGCTG GACGCTACAC TTGTGTGATG 
TCCAATACGC TCGGCACCGA ACGTGCTCAC TCGCAGCTCA CTGTCTACCA GAGATTTGAG 
GAGTGCGCAC CTTTTACCAG CCCCAGCACT GTTACTGTAG GCATCATCAT CATCGCTGTG 
GTCACCAGTA TTGTGGTGAC TTCACTAGTT TGGGTGTGTA TTATCTATCA GACCAGGAAG 
AAGAGCGAGG AGTGCAGTGT AAACACAGAT GAGACGATTG TCCCTCCTGA TGTTCCCAGT 
TACCTGTCCT CTCAGGGCAC TTTATCAGAA AGACAGGACG TGTGTATACG AGTGGAGTCC 
AATGGGGGAT CTCAGCCCAA TGGACGAATA GAAAGCAATG GCTACGATGT GCCGGTCGTG 
TGCGCAGACT GCTTGGAAAA CAGCACCAGC TACTCCAAAC ACTGTAACTA CCTCCCTCAC 
GATTTAGCGC CCCCTTCAGG CATGGAGTAT CAGCAGACGC ACCAAACATC AACTTTCACC 
AGCCTCAGCA ATGAACCACA CTGCAACGGC ACTCCAAACG GCCTTAGAAA AGACAACCAA 
ACACCCATCT TTCCCACCAA CCACGACAGG GTGACAATAT CACCCTCATC AAACCAGTAC 
AGTGACCGAA ATGGATTGTT GGTGAACAAG TCAGACACAC CACTGTCACT GGAGGAGTCG 
TACCATCGGC CAGTAAAACT GCTGTCTGAG GACTGTGACA TTGGGTCAGA GCTCACACAG 
ACTCTATTAC CCAACGGACA CACACACGCG CCCAGTTCAG CTCTAAACCA GAGCCAATCC 
TTTAGGCGGG ACAGTGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329908.1
CDS	188..1708
Translation	MTFTWLKDQETLRQAEVENFAHVRASDGGVMEYTTILHLRHVTFNHEGRYQCIISNHFGSSYSNKARVTVNVLPSFVKTPRDITIRTGTKARLECAAEGHPTPQVAWQKDGGTDFPAARERRMRVMPDDDVFFIMDVKPEDMGVYSCTAKNTAGMISANVTLTVLETPHLAQEMEDRNVVVGETVALQCKALGSPPPRITWLKGEEPLRASDRHHFTPGNQLLVIGSSTLEDAGRYTCVMSNTLGTERAHSQLTVYQRFEECAPFTSPSTVTVGIIIIAVVTSIVVTSLVWVCIIYQTRKKSEECSVNTDETIVPPDVPSYLSSQGTLSERQDVCIRVESNGGSQPNGRIESNGYDVPVVCADCLENSTSYSKHCNYLPHDLAPPSGMEYQQTHQTSTFTSLSNEPHCNGTPNGLRKDNQTPIFPTNHDRVTISPSSNQYSDRNGLLVNKSDTPLSLEESYHRPVKLLSEDCDIGSELTQTLLPNGHTHAPSSALNQSQSFRRDSD

Target ORF information:

RefSeq Version	XM_021474233.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio leucine-rich repeats and immunoglobulin-like domains 1 (lrig1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474233.1

ORF Insert Sequence:

ATGACCTTCA CCTGGCTAAA GGACCAGGAG ACACTCCGCC AGGCTGAGGT GGAGAACTTT 
GCCCATGTGC GGGCCAGCGA TGGAGGAGTG ATGGAGTACA CTACGATCCT TCACCTTCGA 
CACGTCACCT TCAACCATGA GGGACGATAC CAGTGCATCA TCAGCAATCA CTTCGGCTCC 
TCTTACTCCA ACAAGGCCCG CGTCACTGTA AACGTCCTGC CTTCGTTCGT CAAGACCCCA 
CGTGACATCA CCATCCGCAC AGGCACCAAA GCGCGGTTGG AGTGTGCGGC CGAAGGGCAT 
CCGACTCCAC AGGTGGCATG GCAGAAAGAT GGCGGAACAG ACTTCCCTGC TGCCCGAGAG 
CGGCGCATGC GTGTGATGCC TGATGATGAT GTGTTCTTTA TCATGGATGT GAAGCCAGAG 
GATATGGGTG TTTACAGCTG CACAGCGAAA AACACAGCAG GCATGATCTC CGCCAACGTC 
ACCTTAACAG TGCTAGAGAC CCCTCATCTG GCACAGGAAA TGGAGGATCG TAATGTGGTG 
GTAGGCGAAA CTGTAGCACT GCAGTGCAAG GCTTTGGGCA GTCCACCACC TCGCATCACC 
TGGCTGAAGG GTGAAGAGCC TCTTCGAGCC AGCGACCGTC ATCACTTCAC TCCAGGAAAT 
CAGCTGTTGG TGATCGGCAG CAGCACATTA GAGGATGCTG GACGCTACAC TTGTGTGATG 
TCCAATACGC TCGGCACCGA ACGTGCTCAC TCGCAGCTCA CTGTCTACCA GAGATTTGAG 
GAGTGCGCAC CTTTTACCAG CCCCAGCACT GTTACTGTAG GCATCATCAT CATCGCTGTG 
GTCACCAGTA TTGTGGTGAC TTCACTAGTT TGGGTGTGTA TTATCTATCA GACCAGGAAG 
AAGAGCGAGG AGTGCAGTGT AAACACAGAT GAGACGATTG TCCCTCCTGA TGTTCCCAGT 
TACCTGTCCT CTCAGGGCAC TTTATCAGAA AGACAGGACG TGTGTATACG AGTGGAGTCC 
AATGGGGGAT CTCAGCCCAA TGGACGAATA GAAAGCAATG GCTACGATGT GCCGGTCGTG 
TGCGCAGACT GCTTGGAAAA CAGCACCAGC TACTCCAAAC ACTGTAACTA CCTCCCTCAC 
GATTTAGCGC CCCCTTCAGG CATGGAGTAT CAGCAGACGC ACCAAACATC AACTTTCACC 
AGCCTCAGCA ATGAACCACA CTGCAACGGC ACTCCAAACG GCCTTAGAAA AGACAACCAA 
ACACCCATCT TTCCCACCAA CCACGACAGG GTGACAATAT CACCCTCATC AAACCAGTAC 
AGTGACCGAA ATGGATTGTT GGTGAACAAG TCAGACACAC CACTGTCACT GGAGGAGTCG 
TACCATCGGC CAGTAAAACT GCTGTCTGAG GACTGTGACA TTGGGTCAGA GCTCACACAG 
ACTCTATTAC CCAACGGACA CACACACGCG CCCAGTTCAG CTCTAAACCA GAGCCAATCC 
TTTAGGCGGG ACAGTGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRIG1 ( XM_683725.9 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_688817.4 Danio rerio leucine-rich repeats and immunoglobulin-like domains 1 (lrig1), transcript variant X1, mRNA.

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CloneID	ODa16957
Clone ID Related Accession (Same CDS sequence)	XM_683725.9 , XM_683725.8
Accession Version	XM_683725.9 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3069bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	leucine-rich repeats and immunoglobulin-like domains protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_683725.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGT GGCTGGGACA GATGGGATCA CTTTCATATC ATGTGTTTTA TTGGCTTTTG 
ACGCTGGAAC TGTTAATCAA TTATGGCGCA CCGTGTCCTC AAAACTGTAC GTGTACGCTT 
GATACGACCG ACTGCAGTCG TTTGGACATG ACTGATGTCC CTCAAGACTT GCCAAAGAGC 
ACTGTGCATC TAAATCTGAG CCACAACAAG CTGACGGCAG TCGATATGGA CATCCTCTCC 
AATCTGCCCC ACCTGAGAGA AGTGAGGCTT GACCACAATG AGCTGACATC TATCCCTTCA 
TTTGGAGATG CCGCAGCATC AGTTGTTACA CTTCTCCTGC ACCATAATAG AATCCGCAGA 
CTGGAGGGGA GTCTTCTACA GAACTTCTCT GCTCTTGAGA CGCTAGATTT GAGTAATAAT 
GACATCACAG AGTTGAGAGA GCACTGCTTC CCTCTGGGGT TACAAATCAA AGACCTGCAC 
CTGAGCAGTA ATAAGATCGT TCATCTGGAG TTCGGGGCAT TTAAGAATCT TGCCGGGAGT 
CTGCAGATCC TCAGATTGAG CCGGAACCGA TTAACTCATC TTCCTGTTAA AGGCCTGGAG 
CTGCCCAAAC TCACACAACT GGAGTTGAGT AGGAACAGGT TGCGGTTGAT CGAGGGACTC 
ACCTTCCAAG GTTTGAGCAG TTTAGAAGTT CTCAAGCTTC AGAGAAACAA CATCAGCAAA 
CTAACAGATG GGGCATTCTG GGGTCTGGCC CGGATGAGGG TTCTGCACCT GGACTATAAC 
AGTCTACGAG AAGTGAACAG TGGCTCTCTG TATGGTCTGG AGTCTCTGCT GCAGCTCTAC 
CTCGCCAACA ATTCCATCTC AAACTTTAAC CCTGAGGGTT GGGGCTTTTG TGAGAGACTT 
CGAGAACTGA ATTTGTCTTA TAATAACCTG ACGAAGTTGT CTGAGGGTAG TTTTGCCAAG 
CTGGTAAACC TGATTTCCCT GCGTTTGGGA CACAATTCCA TCAGTCACAT CACTGAAGGA 
GCATTCAGAG GCCTTAGCTC CCTGCGCACA CTGGAGTTGG ACCACAATGA CATCTCTGGA 
ACTATTGAGG ACACCAGTGG GGCCTTCACT GGCCTCGAAA ACCTCAACAA GCTAACTCTG 
TTTGGAAACA AGATCAAGTC GGTGGCCAAG AAAGCCTTCT CTGGCCTGGA GGCTCTGGAG 
CACCTGAATT TAGGGGAAAA TGCCATCCGC TCCATCCAAC CCGAGGCATT CAGCAAGATG 
AAGAACCTGC GCTACCTTCA TATTCAGAGC GACAGTTTTC TGTGTGACTG TCAGCTGCAC 
TGGTTTCCAG ATTGGCTCCT CACTCGAGGG CTGAACCCTG GTGTTCAGGC CACATGTGCC 
CATCCAGAGA GCCTAAAGGG CACCAGCATC TACCAAGCCC CACCACAGAG CTTTGTTTGT 
GATGACCTTC CCAAACCCCA GATCACGGTT CACCCAGTCA CCACAGCTGC AGTATTGGGT 
AAAGACGTGC GTCTGACCTG TACTGCTGCC AGCAGCAGCA GTTCTCCCAT GACCTTCACC 
TGGCTAAAGG ACCAGGAGAC ACTCCGCCAG GCTGAGGTGG AGAACTTTGC CCATGTGCGG 
GCCAGCGATG GAGGAGTGAT GGAGTACACT ACGATCCTTC ACCTTCGACA CGTCACCTTC 
AACCATGAGG GACGATACCA GTGCATCATC AGCAATCACT TCGGCTCCTC TTACTCCAAC 
AAGGCCCGCG TCACTGTAAA CGTCCTGCCT TCGTTCGTCA AGACCCCACG TGACATCACC 
ATCCGCACAG GCACCAAAGC GCGGTTGGAG TGTGCGGCCG AAGGGCATCC GACTCCACAG 
GTGGCATGGC AGAAAGATGG CGGAACAGAC TTCCCTGCTG CCCGAGAGCG GCGCATGCGT 
GTGATGCCTG ATGATGATGT GTTCTTTATC ATGGATGTGA AGCCAGAGGA TATGGGTGTT 
TACAGCTGCA CAGCGAAAAA CACAGCAGGC ATGATCTCCG CCAACGTCAC CTTAACAGTG 
CTAGAGACCC CTCATCTGGC ACAGGAAATG GAGGATCGTA ATGTGGTGGT AGGCGAAACT 
GTAGCACTGC AGTGCAAGGC TTTGGGCAGT CCACCACCTC GCATCACCTG GCTGAAGGGT 
GAAGAGCCTC TTCGAGCCAG CGACCGTCAT CACTTCACTC CAGGAAATCA GCTGTTGGTG 
ATCGGCAGCA GCACATTAGA GGATGCTGGA CGCTACACTT GTGTGATGTC CAATACGCTC 
GGCACCGAAC GTGCTCACTC GCAGCTCACT GTCTACCAGA GATTTGAGGA GTGCGCACCT 
TTTACCAGCC CCAGCACTGT TACTGTAGGC ATCATCATCA TCGCTGTGGT CACCAGTATT 
GTGGTGACTT CACTAGTTTG GGTGTGTATT ATCTATCAGA CCAGGAAGAA GAGCGAGGAG 
TGCAGTGTAA ACACAGATGA GACGATTGTC CCTCCTGATG TTCCCAGTTA CCTGTCCTCT 
CAGGGCACTT TATCAGAAAG ACAGGACGTG TGTATACGAG TGGAGTCCAA TGGGGGATCT 
CAGCCCAATG GACGAATAGA AAGCAATGGC TACGATGTGC CGGTCGTGTG CGCAGACTGC 
TTGGAAAACA GCACCAGCTA CTCCAAACAC TGTAACTACC TCCCTCACGA TTTAGCGCCC 
CCTTCAGGCA TGGAGTATCA GCAGACGCAC CAAACATCAA CTTTCACCAG CCTCAGCAAT 
GAACCACACT GCAACGGCAC TCCAAACGGC CTTAGAAAAG ACAACCAAAC ACCCATCTTT 
CCCACCAACC ACGACAGGGT GACAATATCA CCCTCATCAA ACCAGTACAG TGACCGAAAT 
GGATTGTTGG TGAACAAGTC AGACACACCA CTGTCACTGG AGGAGTCGTA CCATCGGCCA 
GTAAAACTGC TGTCTGAGGA CTGTGACATT GGGTCAGAGC TCACACAGAC TCTATTACCC 
AACGGACACA CACACGCGCC CAGTTCAGCT CTAAACCAGA GCCAATCCTT TAGGCGGGAC 
AGTGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_688817.4
CDS	928..3996
Translation	MAAWLGQMGSLSYHVFYWLLTLELLINYGAPCPQNCTCTLDTTDCSRLDMTDVPQDLPKSTVHLNLSHNKLTAVDMDILSNLPHLREVRLDHNELTSIPSFGDAAASVVTLLLHHNRIRRLEGSLLQNFSALETLDLSNNDITELREHCFPLGLQIKDLHLSSNKIVHLEFGAFKNLAGSLQILRLSRNRLTHLPVKGLELPKLTQLELSRNRLRLIEGLTFQGLSSLEVLKLQRNNISKLTDGAFWGLARMRVLHLDYNSLREVNSGSLYGLESLLQLYLANNSISNFNPEGWGFCERLRELNLSYNNLTKLSEGSFAKLVNLISLRLGHNSISHITEGAFRGLSSLRTLELDHNDISGTIEDTSGAFTGLENLNKLTLFGNKIKSVAKKAFSGLEALEHLNLGENAIRSIQPEAFSKMKNLRYLHIQSDSFLCDCQLHWFPDWLLTRGLNPGVQATCAHPESLKGTSIYQAPPQSFVCDDLPKPQITVHPVTTAAVLGKDVRLTCTAASSSSSPMTFTWLKDQETLRQAEVENFAHVRASDGGVMEYTTILHLRHVTFNHEGRYQCIISNHFGSSYSNKARVTVNVLPSFVKTPRDITIRTGTKARLECAAEGHPTPQVAWQKDGGTDFPAARERRMRVMPDDDVFFIMDVKPEDMGVYSCTAKNTAGMISANVTLTVLETPHLAQEMEDRNVVVGETVALQCKALGSPPPRITWLKGEEPLRASDRHHFTPGNQLLVIGSSTLEDAGRYTCVMSNTLGTERAHSQLTVYQRFEECAPFTSPSTVTVGIIIIAVVTSIVVTSLVWVCIIYQTRKKSEECSVNTDETIVPPDVPSYLSSQGTLSERQDVCIRVESNGGSQPNGRIESNGYDVPVVCADCLENSTSYSKHCNYLPHDLAPPSGMEYQQTHQTSTFTSLSNEPHCNGTPNGLRKDNQTPIFPTNHDRVTISPSSNQYSDRNGLLVNKSDTPLSLEESYHRPVKLLSEDCDIGSELTQTLLPNGHTHAPSSALNQSQSFRRDSD

Target ORF information:

RefSeq Version	XM_683725.9
Organism	Danio rerio(zebrafish)
Definition	Danio rerio leucine-rich repeats and immunoglobulin-like domains 1 (lrig1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_683725.9

ORF Insert Sequence:

ATGGCGGCGT GGCTGGGACA GATGGGATCA CTTTCATATC ATGTGTTTTA TTGGCTTTTG 
ACGCTGGAAC TGTTAATCAA TTATGGCGCA CCGTGTCCTC AAAACTGTAC GTGTACGCTT 
GATACGACCG ACTGCAGTCG TTTGGACATG ACTGATGTCC CTCAAGACTT GCCAAAGAGC 
ACTGTGCATC TAAATCTGAG CCACAACAAG CTGACGGCAG TCGATATGGA CATCCTCTCC 
AATCTGCCCC ACCTGAGAGA AGTGAGGCTT GACCACAATG AGCTGACATC TATCCCTTCA 
TTTGGAGATG CCGCAGCATC AGTTGTTACA CTTCTCCTGC ACCATAATAG AATCCGCAGA 
CTGGAGGGGA GTCTTCTACA GAACTTCTCT GCTCTTGAGA CGCTAGATTT GAGTAATAAT 
GACATCACAG AGTTGAGAGA GCACTGCTTC CCTCTGGGGT TACAAATCAA AGACCTGCAC 
CTGAGCAGTA ATAAGATCGT TCATCTGGAG TTCGGGGCAT TTAAGAATCT TGCCGGGAGT 
CTGCAGATCC TCAGATTGAG CCGGAACCGA TTAACTCATC TTCCTGTTAA AGGCCTGGAG 
CTGCCCAAAC TCACACAACT GGAGTTGAGT AGGAACAGGT TGCGGTTGAT CGAGGGACTC 
ACCTTCCAAG GTTTGAGCAG TTTAGAAGTT CTCAAGCTTC AGAGAAACAA CATCAGCAAA 
CTAACAGATG GGGCATTCTG GGGTCTGGCC CGGATGAGGG TTCTGCACCT GGACTATAAC 
AGTCTACGAG AAGTGAACAG TGGCTCTCTG TATGGTCTGG AGTCTCTGCT GCAGCTCTAC 
CTCGCCAACA ATTCCATCTC AAACTTTAAC CCTGAGGGTT GGGGCTTTTG TGAGAGACTT 
CGAGAACTGA ATTTGTCTTA TAATAACCTG ACGAAGTTGT CTGAGGGTAG TTTTGCCAAG 
CTGGTAAACC TGATTTCCCT GCGTTTGGGA CACAATTCCA TCAGTCACAT CACTGAAGGA 
GCATTCAGAG GCCTTAGCTC CCTGCGCACA CTGGAGTTGG ACCACAATGA CATCTCTGGA 
ACTATTGAGG ACACCAGTGG GGCCTTCACT GGCCTCGAAA ACCTCAACAA GCTAACTCTG 
TTTGGAAACA AGATCAAGTC GGTGGCCAAG AAAGCCTTCT CTGGCCTGGA GGCTCTGGAG 
CACCTGAATT TAGGGGAAAA TGCCATCCGC TCCATCCAAC CCGAGGCATT CAGCAAGATG 
AAGAACCTGC GCTACCTTCA TATTCAGAGC GACAGTTTTC TGTGTGACTG TCAGCTGCAC 
TGGTTTCCAG ATTGGCTCCT CACTCGAGGG CTGAACCCTG GTGTTCAGGC CACATGTGCC 
CATCCAGAGA GCCTAAAGGG CACCAGCATC TACCAAGCCC CACCACAGAG CTTTGTTTGT 
GATGACCTTC CCAAACCCCA GATCACGGTT CACCCAGTCA CCACAGCTGC AGTATTGGGT 
AAAGACGTGC GTCTGACCTG TACTGCTGCC AGCAGCAGCA GTTCTCCCAT GACCTTCACC 
TGGCTAAAGG ACCAGGAGAC ACTCCGCCAG GCTGAGGTGG AGAACTTTGC CCATGTGCGG 
GCCAGCGATG GAGGAGTGAT GGAGTACACT ACGATCCTTC ACCTTCGACA CGTCACCTTC 
AACCATGAGG GACGATACCA GTGCATCATC AGCAATCACT TCGGCTCCTC TTACTCCAAC 
AAGGCCCGCG TCACTGTAAA CGTCCTGCCT TCGTTCGTCA AGACCCCACG TGACATCACC 
ATCCGCACAG GCACCAAAGC GCGGTTGGAG TGTGCGGCCG AAGGGCATCC GACTCCACAG 
GTGGCATGGC AGAAAGATGG CGGAACAGAC TTCCCTGCTG CCCGAGAGCG GCGCATGCGT 
GTGATGCCTG ATGATGATGT GTTCTTTATC ATGGATGTGA AGCCAGAGGA TATGGGTGTT 
TACAGCTGCA CAGCGAAAAA CACAGCAGGC ATGATCTCCG CCAACGTCAC CTTAACAGTG 
CTAGAGACCC CTCATCTGGC ACAGGAAATG GAGGATCGTA ATGTGGTGGT AGGCGAAACT 
GTAGCACTGC AGTGCAAGGC TTTGGGCAGT CCACCACCTC GCATCACCTG GCTGAAGGGT 
GAAGAGCCTC TTCGAGCCAG CGACCGTCAT CACTTCACTC CAGGAAATCA GCTGTTGGTG 
ATCGGCAGCA GCACATTAGA GGATGCTGGA CGCTACACTT GTGTGATGTC CAATACGCTC 
GGCACCGAAC GTGCTCACTC GCAGCTCACT GTCTACCAGA GATTTGAGGA GTGCGCACCT 
TTTACCAGCC CCAGCACTGT TACTGTAGGC ATCATCATCA TCGCTGTGGT CACCAGTATT 
GTGGTGACTT CACTAGTTTG GGTGTGTATT ATCTATCAGA CCAGGAAGAA GAGCGAGGAG 
TGCAGTGTAA ACACAGATGA GACGATTGTC CCTCCTGATG TTCCCAGTTA CCTGTCCTCT 
CAGGGCACTT TATCAGAAAG ACAGGACGTG TGTATACGAG TGGAGTCCAA TGGGGGATCT 
CAGCCCAATG GACGAATAGA AAGCAATGGC TACGATGTGC CGGTCGTGTG CGCAGACTGC 
TTGGAAAACA GCACCAGCTA CTCCAAACAC TGTAACTACC TCCCTCACGA TTTAGCGCCC 
CCTTCAGGCA TGGAGTATCA GCAGACGCAC CAAACATCAA CTTTCACCAG CCTCAGCAAT 
GAACCACACT GCAACGGCAC TCCAAACGGC CTTAGAAAAG ACAACCAAAC ACCCATCTTT 
CCCACCAACC ACGACAGGGT GACAATATCA CCCTCATCAA ACCAGTACAG TGACCGAAAT 
GGATTGTTGG TGAACAAGTC AGACACACCA CTGTCACTGG AGGAGTCGTA CCATCGGCCA 
GTAAAACTGC TGTCTGAGGA CTGTGACATT GGGTCAGAGC TCACACAGAC TCTATTACCC 
AACGGACACA CACACGCGCC CAGTTCAGCT CTAAACCAGA GCCAATCCTT TAGGCGGGAC 
AGTGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRIG1 ( XM_683725.8 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_688817.4 Danio rerio leucine-rich repeats and immunoglobulin-like domains 1 (lrig1), transcript variant X1, mRNA.

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CloneID	ODa16957
Clone ID Related Accession (Same CDS sequence)	XM_683725.9 , XM_683725.8
Accession Version	XM_683725.8	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3069bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	leucine-rich repeats and immunoglobulin-like domains protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_683725.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGT GGCTGGGACA GATGGGATCA CTTTCATATC ATGTGTTTTA TTGGCTTTTG 
ACGCTGGAAC TGTTAATCAA TTATGGCGCA CCGTGTCCTC AAAACTGTAC GTGTACGCTT 
GATACGACCG ACTGCAGTCG TTTGGACATG ACTGATGTCC CTCAAGACTT GCCAAAGAGC 
ACTGTGCATC TAAATCTGAG CCACAACAAG CTGACGGCAG TCGATATGGA CATCCTCTCC 
AATCTGCCCC ACCTGAGAGA AGTGAGGCTT GACCACAATG AGCTGACATC TATCCCTTCA 
TTTGGAGATG CCGCAGCATC AGTTGTTACA CTTCTCCTGC ACCATAATAG AATCCGCAGA 
CTGGAGGGGA GTCTTCTACA GAACTTCTCT GCTCTTGAGA CGCTAGATTT GAGTAATAAT 
GACATCACAG AGTTGAGAGA GCACTGCTTC CCTCTGGGGT TACAAATCAA AGACCTGCAC 
CTGAGCAGTA ATAAGATCGT TCATCTGGAG TTCGGGGCAT TTAAGAATCT TGCCGGGAGT 
CTGCAGATCC TCAGATTGAG CCGGAACCGA TTAACTCATC TTCCTGTTAA AGGCCTGGAG 
CTGCCCAAAC TCACACAACT GGAGTTGAGT AGGAACAGGT TGCGGTTGAT CGAGGGACTC 
ACCTTCCAAG GTTTGAGCAG TTTAGAAGTT CTCAAGCTTC AGAGAAACAA CATCAGCAAA 
CTAACAGATG GGGCATTCTG GGGTCTGGCC CGGATGAGGG TTCTGCACCT GGACTATAAC 
AGTCTACGAG AAGTGAACAG TGGCTCTCTG TATGGTCTGG AGTCTCTGCT GCAGCTCTAC 
CTCGCCAACA ATTCCATCTC AAACTTTAAC CCTGAGGGTT GGGGCTTTTG TGAGAGACTT 
CGAGAACTGA ATTTGTCTTA TAATAACCTG ACGAAGTTGT CTGAGGGTAG TTTTGCCAAG 
CTGGTAAACC TGATTTCCCT GCGTTTGGGA CACAATTCCA TCAGTCACAT CACTGAAGGA 
GCATTCAGAG GCCTTAGCTC CCTGCGCACA CTGGAGTTGG ACCACAATGA CATCTCTGGA 
ACTATTGAGG ACACCAGTGG GGCCTTCACT GGCCTCGAAA ACCTCAACAA GCTAACTCTG 
TTTGGAAACA AGATCAAGTC GGTGGCCAAG AAAGCCTTCT CTGGCCTGGA GGCTCTGGAG 
CACCTGAATT TAGGGGAAAA TGCCATCCGC TCCATCCAAC CCGAGGCATT CAGCAAGATG 
AAGAACCTGC GCTACCTTCA TATTCAGAGC GACAGTTTTC TGTGTGACTG TCAGCTGCAC 
TGGTTTCCAG ATTGGCTCCT CACTCGAGGG CTGAACCCTG GTGTTCAGGC CACATGTGCC 
CATCCAGAGA GCCTAAAGGG CACCAGCATC TACCAAGCCC CACCACAGAG CTTTGTTTGT 
GATGACCTTC CCAAACCCCA GATCACGGTT CACCCAGTCA CCACAGCTGC AGTATTGGGT 
AAAGACGTGC GTCTGACCTG TACTGCTGCC AGCAGCAGCA GTTCTCCCAT GACCTTCACC 
TGGCTAAAGG ACCAGGAGAC ACTCCGCCAG GCTGAGGTGG AGAACTTTGC CCATGTGCGG 
GCCAGCGATG GAGGAGTGAT GGAGTACACT ACGATCCTTC ACCTTCGACA CGTCACCTTC 
AACCATGAGG GACGATACCA GTGCATCATC AGCAATCACT TCGGCTCCTC TTACTCCAAC 
AAGGCCCGCG TCACTGTAAA CGTCCTGCCT TCGTTCGTCA AGACCCCACG TGACATCACC 
ATCCGCACAG GCACCAAAGC GCGGTTGGAG TGTGCGGCCG AAGGGCATCC GACTCCACAG 
GTGGCATGGC AGAAAGATGG CGGAACAGAC TTCCCTGCTG CCCGAGAGCG GCGCATGCGT 
GTGATGCCTG ATGATGATGT GTTCTTTATC ATGGATGTGA AGCCAGAGGA TATGGGTGTT 
TACAGCTGCA CAGCGAAAAA CACAGCAGGC ATGATCTCCG CCAACGTCAC CTTAACAGTG 
CTAGAGACCC CTCATCTGGC ACAGGAAATG GAGGATCGTA ATGTGGTGGT AGGCGAAACT 
GTAGCACTGC AGTGCAAGGC TTTGGGCAGT CCACCACCTC GCATCACCTG GCTGAAGGGT 
GAAGAGCCTC TTCGAGCCAG CGACCGTCAT CACTTCACTC CAGGAAATCA GCTGTTGGTG 
ATCGGCAGCA GCACATTAGA GGATGCTGGA CGCTACACTT GTGTGATGTC CAATACGCTC 
GGCACCGAAC GTGCTCACTC GCAGCTCACT GTCTACCAGA GATTTGAGGA GTGCGCACCT 
TTTACCAGCC CCAGCACTGT TACTGTAGGC ATCATCATCA TCGCTGTGGT CACCAGTATT 
GTGGTGACTT CACTAGTTTG GGTGTGTATT ATCTATCAGA CCAGGAAGAA GAGCGAGGAG 
TGCAGTGTAA ACACAGATGA GACGATTGTC CCTCCTGATG TTCCCAGTTA CCTGTCCTCT 
CAGGGCACTT TATCAGAAAG ACAGGACGTG TGTATACGAG TGGAGTCCAA TGGGGGATCT 
CAGCCCAATG GACGAATAGA AAGCAATGGC TACGATGTGC CGGTCGTGTG CGCAGACTGC 
TTGGAAAACA GCACCAGCTA CTCCAAACAC TGTAACTACC TCCCTCACGA TTTAGCGCCC 
CCTTCAGGCA TGGAGTATCA GCAGACGCAC CAAACATCAA CTTTCACCAG CCTCAGCAAT 
GAACCACACT GCAACGGCAC TCCAAACGGC CTTAGAAAAG ACAACCAAAC ACCCATCTTT 
CCCACCAACC ACGACAGGGT GACAATATCA CCCTCATCAA ACCAGTACAG TGACCGAAAT 
GGATTGTTGG TGAACAAGTC AGACACACCA CTGTCACTGG AGGAGTCGTA CCATCGGCCA 
GTAAAACTGC TGTCTGAGGA CTGTGACATT GGGTCAGAGC TCACACAGAC TCTATTACCC 
AACGGACACA CACACGCGCC CAGTTCAGCT CTAAACCAGA GCCAATCCTT TAGGCGGGAC 
AGTGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_688817.4
CDS	922..3990
Translation	MAAWLGQMGSLSYHVFYWLLTLELLINYGAPCPQNCTCTLDTTDCSRLDMTDVPQDLPKSTVHLNLSHNKLTAVDMDILSNLPHLREVRLDHNELTSIPSFGDAAASVVTLLLHHNRIRRLEGSLLQNFSALETLDLSNNDITELREHCFPLGLQIKDLHLSSNKIVHLEFGAFKNLAGSLQILRLSRNRLTHLPVKGLELPKLTQLELSRNRLRLIEGLTFQGLSSLEVLKLQRNNISKLTDGAFWGLARMRVLHLDYNSLREVNSGSLYGLESLLQLYLANNSISNFNPEGWGFCERLRELNLSYNNLTKLSEGSFAKLVNLISLRLGHNSISHITEGAFRGLSSLRTLELDHNDISGTIEDTSGAFTGLENLNKLTLFGNKIKSVAKKAFSGLEALEHLNLGENAIRSIQPEAFSKMKNLRYLHIQSDSFLCDCQLHWFPDWLLTRGLNPGVQATCAHPESLKGTSIYQAPPQSFVCDDLPKPQITVHPVTTAAVLGKDVRLTCTAASSSSSPMTFTWLKDQETLRQAEVENFAHVRASDGGVMEYTTILHLRHVTFNHEGRYQCIISNHFGSSYSNKARVTVNVLPSFVKTPRDITIRTGTKARLECAAEGHPTPQVAWQKDGGTDFPAARERRMRVMPDDDVFFIMDVKPEDMGVYSCTAKNTAGMISANVTLTVLETPHLAQEMEDRNVVVGETVALQCKALGSPPPRITWLKGEEPLRASDRHHFTPGNQLLVIGSSTLEDAGRYTCVMSNTLGTERAHSQLTVYQRFEECAPFTSPSTVTVGIIIIAVVTSIVVTSLVWVCIIYQTRKKSEECSVNTDETIVPPDVPSYLSSQGTLSERQDVCIRVESNGGSQPNGRIESNGYDVPVVCADCLENSTSYSKHCNYLPHDLAPPSGMEYQQTHQTSTFTSLSNEPHCNGTPNGLRKDNQTPIFPTNHDRVTISPSSNQYSDRNGLLVNKSDTPLSLEESYHRPVKLLSEDCDIGSELTQTLLPNGHTHAPSSALNQSQSFRRDSD

Target ORF information:

RefSeq Version	XM_683725.8
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio leucine-rich repeats and immunoglobulin-like domains 1 (lrig1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_683725.8

ORF Insert Sequence:

ATGGCGGCGT GGCTGGGACA GATGGGATCA CTTTCATATC ATGTGTTTTA TTGGCTTTTG 
ACGCTGGAAC TGTTAATCAA TTATGGCGCA CCGTGTCCTC AAAACTGTAC GTGTACGCTT 
GATACGACCG ACTGCAGTCG TTTGGACATG ACTGATGTCC CTCAAGACTT GCCAAAGAGC 
ACTGTGCATC TAAATCTGAG CCACAACAAG CTGACGGCAG TCGATATGGA CATCCTCTCC 
AATCTGCCCC ACCTGAGAGA AGTGAGGCTT GACCACAATG AGCTGACATC TATCCCTTCA 
TTTGGAGATG CCGCAGCATC AGTTGTTACA CTTCTCCTGC ACCATAATAG AATCCGCAGA 
CTGGAGGGGA GTCTTCTACA GAACTTCTCT GCTCTTGAGA CGCTAGATTT GAGTAATAAT 
GACATCACAG AGTTGAGAGA GCACTGCTTC CCTCTGGGGT TACAAATCAA AGACCTGCAC 
CTGAGCAGTA ATAAGATCGT TCATCTGGAG TTCGGGGCAT TTAAGAATCT TGCCGGGAGT 
CTGCAGATCC TCAGATTGAG CCGGAACCGA TTAACTCATC TTCCTGTTAA AGGCCTGGAG 
CTGCCCAAAC TCACACAACT GGAGTTGAGT AGGAACAGGT TGCGGTTGAT CGAGGGACTC 
ACCTTCCAAG GTTTGAGCAG TTTAGAAGTT CTCAAGCTTC AGAGAAACAA CATCAGCAAA 
CTAACAGATG GGGCATTCTG GGGTCTGGCC CGGATGAGGG TTCTGCACCT GGACTATAAC 
AGTCTACGAG AAGTGAACAG TGGCTCTCTG TATGGTCTGG AGTCTCTGCT GCAGCTCTAC 
CTCGCCAACA ATTCCATCTC AAACTTTAAC CCTGAGGGTT GGGGCTTTTG TGAGAGACTT 
CGAGAACTGA ATTTGTCTTA TAATAACCTG ACGAAGTTGT CTGAGGGTAG TTTTGCCAAG 
CTGGTAAACC TGATTTCCCT GCGTTTGGGA CACAATTCCA TCAGTCACAT CACTGAAGGA 
GCATTCAGAG GCCTTAGCTC CCTGCGCACA CTGGAGTTGG ACCACAATGA CATCTCTGGA 
ACTATTGAGG ACACCAGTGG GGCCTTCACT GGCCTCGAAA ACCTCAACAA GCTAACTCTG 
TTTGGAAACA AGATCAAGTC GGTGGCCAAG AAAGCCTTCT CTGGCCTGGA GGCTCTGGAG 
CACCTGAATT TAGGGGAAAA TGCCATCCGC TCCATCCAAC CCGAGGCATT CAGCAAGATG 
AAGAACCTGC GCTACCTTCA TATTCAGAGC GACAGTTTTC TGTGTGACTG TCAGCTGCAC 
TGGTTTCCAG ATTGGCTCCT CACTCGAGGG CTGAACCCTG GTGTTCAGGC CACATGTGCC 
CATCCAGAGA GCCTAAAGGG CACCAGCATC TACCAAGCCC CACCACAGAG CTTTGTTTGT 
GATGACCTTC CCAAACCCCA GATCACGGTT CACCCAGTCA CCACAGCTGC AGTATTGGGT 
AAAGACGTGC GTCTGACCTG TACTGCTGCC AGCAGCAGCA GTTCTCCCAT GACCTTCACC 
TGGCTAAAGG ACCAGGAGAC ACTCCGCCAG GCTGAGGTGG AGAACTTTGC CCATGTGCGG 
GCCAGCGATG GAGGAGTGAT GGAGTACACT ACGATCCTTC ACCTTCGACA CGTCACCTTC 
AACCATGAGG GACGATACCA GTGCATCATC AGCAATCACT TCGGCTCCTC TTACTCCAAC 
AAGGCCCGCG TCACTGTAAA CGTCCTGCCT TCGTTCGTCA AGACCCCACG TGACATCACC 
ATCCGCACAG GCACCAAAGC GCGGTTGGAG TGTGCGGCCG AAGGGCATCC GACTCCACAG 
GTGGCATGGC AGAAAGATGG CGGAACAGAC TTCCCTGCTG CCCGAGAGCG GCGCATGCGT 
GTGATGCCTG ATGATGATGT GTTCTTTATC ATGGATGTGA AGCCAGAGGA TATGGGTGTT 
TACAGCTGCA CAGCGAAAAA CACAGCAGGC ATGATCTCCG CCAACGTCAC CTTAACAGTG 
CTAGAGACCC CTCATCTGGC ACAGGAAATG GAGGATCGTA ATGTGGTGGT AGGCGAAACT 
GTAGCACTGC AGTGCAAGGC TTTGGGCAGT CCACCACCTC GCATCACCTG GCTGAAGGGT 
GAAGAGCCTC TTCGAGCCAG CGACCGTCAT CACTTCACTC CAGGAAATCA GCTGTTGGTG 
ATCGGCAGCA GCACATTAGA GGATGCTGGA CGCTACACTT GTGTGATGTC CAATACGCTC 
GGCACCGAAC GTGCTCACTC GCAGCTCACT GTCTACCAGA GATTTGAGGA GTGCGCACCT 
TTTACCAGCC CCAGCACTGT TACTGTAGGC ATCATCATCA TCGCTGTGGT CACCAGTATT 
GTGGTGACTT CACTAGTTTG GGTGTGTATT ATCTATCAGA CCAGGAAGAA GAGCGAGGAG 
TGCAGTGTAA ACACAGATGA GACGATTGTC CCTCCTGATG TTCCCAGTTA CCTGTCCTCT 
CAGGGCACTT TATCAGAAAG ACAGGACGTG TGTATACGAG TGGAGTCCAA TGGGGGATCT 
CAGCCCAATG GACGAATAGA AAGCAATGGC TACGATGTGC CGGTCGTGTG CGCAGACTGC 
TTGGAAAACA GCACCAGCTA CTCCAAACAC TGTAACTACC TCCCTCACGA TTTAGCGCCC 
CCTTCAGGCA TGGAGTATCA GCAGACGCAC CAAACATCAA CTTTCACCAG CCTCAGCAAT 
GAACCACACT GCAACGGCAC TCCAAACGGC CTTAGAAAAG ACAACCAAAC ACCCATCTTT 
CCCACCAACC ACGACAGGGT GACAATATCA CCCTCATCAA ACCAGTACAG TGACCGAAAT 
GGATTGTTGG TGAACAAGTC AGACACACCA CTGTCACTGG AGGAGTCGTA CCATCGGCCA 
GTAAAACTGC TGTCTGAGGA CTGTGACATT GGGTCAGAGC TCACACAGAC TCTATTACCC 
AACGGACACA CACACGCGCC CAGTTCAGCT CTAAACCAGA GCCAATCCTT TAGGCGGGAC 
AGTGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LRIG1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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